LOCUS       NZ_CP098209            14844 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain Z0117EC0054 plasmid pZ0117ECO054-3, complete
            sequence.
ACCESSION   NZ_CP098209
VERSION     NZ_CP098209.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28703748
            Assembly: GCF_023658305.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14844)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Klebsiella pneumoniae, a One Health interface shuttle organism for
            the genes of CTX-M ESBL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14844)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2022) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v10.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 126x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023658305.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 04:27:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,312
            CDSs (total)                      :: 5,193
            Genes (coding)                    :: 4,946
            CDSs (with protein)               :: 4,946
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 104 of 247
            Pseudo Genes (incomplete)         :: 151 of 247
            Pseudo Genes (internal stop)      :: 54 of 247
            Pseudo Genes (multiple problems)  :: 54 of 247
            Pseudo Genes (short protein)      :: 1 of 247
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP098209.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..14844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z0117EC0054"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:562"
                     /plasmid="pZ0117ECO054-3"
                     /geo_loc_name="South Korea"
                     /collection_date="2017-08"
     gene            join(<14831..14844,1..200)
                     /locus_tag="NBY18_RS26395"
                     /old_locus_tag="NBY18_26385"
                     /pseudo=""
     CDS             join(<14831..14844,1..200)
                     /locus_tag="NBY18_RS26395"
                     /old_locus_tag="NBY18_26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /name="DUF1398 family protein"
     gene            complement(464..>631)
                     /locus_tag="NBY18_RS26400"
                     /old_locus_tag="NBY18_26390"
                     /pseudo=""
     CDS             complement(464..>631)
                     /locus_tag="NBY18_RS26400"
                     /old_locus_tag="NBY18_26390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309635.1"
                     /note="Converts isocitrate to alpha ketoglutarate; internal
                     stop; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /name="NADP-dependent isocitrate dehydrogenase"
     gene            2542..3455
                     /gene="sitA"
                     /locus_tag="NBY18_RS26405"
                     /old_locus_tag="NBY18_26395"
                     /pseudo=""
     CDS             2542..3455
                     /gene="sitA"
                     /locus_tag="NBY18_RS26405"
                     /old_locus_tag="NBY18_26395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /name="sitA"
     gene            3455..4282
                     /gene="sitB"
                     /locus_tag="NBY18_RS26410"
                     /old_locus_tag="NBY18_26400"
     CDS             3455..4282
                     /gene="sitB"
                     /locus_tag="NBY18_RS26410"
                     /old_locus_tag="NBY18_26400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="WP_000983710.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPGGSIAALVGVNGSG
                     KSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVMMG
                     RYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQGDV
                     ILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGTVLAS
                     GPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
                     /name="sitB"
     gene            4342..4799
                     /locus_tag="NBY18_RS26415"
                     /pseudo=""
     CDS             4342..4799
                     /locus_tag="NBY18_RS26415"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]; GO:0005886 - plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /name="metal ABC transporter permease"
     gene            4781..5131
                     /locus_tag="NBY18_RS26420"
     CDS             4781..5131
                     /locus_tag="NBY18_RS26420"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]; GO:0005886 - plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="WP_250789186.1"
                     /translation="MHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLT
                     DRFPRLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLAN
                     RRRAHKALEDRS"
                     /name="metal ABC transporter permease"
     gene            5128..5985
                     /gene="sitD"
                     /locus_tag="NBY18_RS26425"
                     /old_locus_tag="NBY18_26410"
     CDS             5128..5985
                     /gene="sitD"
                     /locus_tag="NBY18_RS26425"
                     /old_locus_tag="NBY18_26410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="WP_000968139.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKSWALMGD
                     AMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGMFA
                     AGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFSFDY
                     QQAQASGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRFHIAL
                     LLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVMFIMTFTVTSINARTKENAEPRDL
                     LSSD"
                     /name="sitD"
     gene            complement(6219..>6476)
                     /locus_tag="NBY18_RS26430"
                     /old_locus_tag="NBY18_26415"
                     /pseudo=""
     CDS             complement(6219..>6476)
                     /locus_tag="NBY18_RS26430"
                     /old_locus_tag="NBY18_26415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            <6474..6887
                     /locus_tag="NBY18_RS26435"
                     /old_locus_tag="NBY18_26420"
                     /pseudo=""
     CDS             <6474..6887
                     /locus_tag="NBY18_RS26435"
                     /old_locus_tag="NBY18_26420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /name="phosphopyruvate hydratase"
     gene            7228..7608
                     /gene="crcB"
                     /locus_tag="NBY18_RS26440"
                     /old_locus_tag="NBY18_26425"
     CDS             7228..7608
                     /gene="crcB"
                     /locus_tag="NBY18_RS26440"
                     /old_locus_tag="NBY18_26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="WP_001318207.1"
                     /translation="MIKSLFAVIIGGSVGCTLRWLLSTRFNSLFPNLPPGTLVVNLLAG
                     LIIGTALAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQAGNYIWALTSVL
                     VHVIGSLIMTALGFFIITILFA"
                     /name="crcB"
     gene            complement(7987..9180)
                     /locus_tag="NBY18_RS26445"
                     /old_locus_tag="NBY18_26430"
     CDS             complement(7987..9180)
                     /locus_tag="NBY18_RS26445"
                     /old_locus_tag="NBY18_26430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="WP_000095526.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTNL
                     SLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLMAT
                     GAVTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSCGRL
                     FGPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDCLPYL
                     LCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVVLRPQR
                     LTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLNDKLADGA
                     GAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWHYRTIK"
                     /name="MFS transporter"
     gene            9316..11036
                     /gene="iucA"
                     /locus_tag="NBY18_RS26450"
                     /old_locus_tag="NBY18_26435"
                     /pseudo=""
     ARG             9317..10989
                     /name="iucA"
                     /gene="iucA"
                     /gene_name="aerobactin synthase IucA"
                     /tool="amrfinderplus"
                     /drug_class="nan"
                     /identity="95.18"
                     /coverage="97.22"
     gene            11037..11983
                     /gene="iucB"
                     /locus_tag="NBY18_RS26455"
                     /old_locus_tag="NBY18_26440"
                     /pseudo=""
     CDS             11037..11983
                     /gene="iucB"
                     /locus_tag="NBY18_RS26455"
                     /old_locus_tag="NBY18_26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /name="iucB"
     gene            11983..13726
                     /gene="iucC"
                     /locus_tag="NBY18_RS26460"
                     /old_locus_tag="NBY18_26445"
                     /pseudo=""
     ARG             11984..13587
                     /name="iucC"
                     /gene="iucC"
                     /gene_name="NIS family aerobactin synthetase IucC"
                     /tool="amrfinderplus"
                     /drug_class="nan"
                     /identity="95.51"
                     /coverage="92.24"
     gene            13723..>14841
                     /gene="iucD"
                     /locus_tag="NBY18_RS26465"
                     /old_locus_tag="NBY18_26450"
                     /pseudo=""
     CDS             13723..>14841
                     /gene="iucD"
                     /locus_tag="NBY18_RS26465"
                     /old_locus_tag="NBY18_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase IucD"
                     /name="iucD"
     BGC             1..14844
                     /name="NI-siderophore"
                     /gene="NI-siderophore"
                     /tool="antismash"
ORIGIN
        1 caagaaccca gctaaaataa aagaggccgc attgcttcat attcgcggaa aatctacatt
       61 tcgggaatac tgttcaacac tggcaggcgc tgcgttttcc ggtgggttac tgatgttaat
      121 cataacaaaa cgcagttact acgccatgat aatacgcttt atatattgaa gatgtagaaa
      181 ataataaacc attaatctag ctaaagttgg gagacttaaa aaatgcttca taattcagta
      241 aggcattagc ataatggaaa taaaagtgca gagactatcc tatggatgat taatactgtc
      301 ttttattgct ccccataaat aatcaccaga ccaatacgat caaaattgat attttgaaaa
      361 tgtgttcact tgacttttga tacgttattt tataacggtt aacatattta taaaaacaac
      421 gggcgtgtta tacgcccgtt tcaatattta acacatgtag agattacatg ttcttgatga
      481 tcgcgtcacc aaactctgaa catttcagca gtttagcgcc ttccatcaga cgttcgaagt
      541 cataggttac ggtcttcgcg ttgattgcac cttcccatac ctttaacaat cagtctgcgg
      601 cttcagtcca gcccatatgg cgcagcagta tataaaaatt atgtattcaa ctcgctgata
      661 tttaatcata aatattattt cttgtctttg tattagccaa aatcaacttt attcaaccgt
      721 ttgatttcat tgacgttcgt ttttattttg gggaaagatt ttctctacca tttatatcaa
      781 aattacaagt ctacatttta ttaacatctc aatacatgtg tggccttgca agccattgcg
      841 aggccttatg tgtctcagtt ttgtccgcct tgtattacga cttgcatagc caatgaagat
      901 aaacgtgacg acaaacaaag cagctgtctt cttttctttc acactttccc cactcagcag
      961 catgcatacc ttctactata actgtagtga atgtcttaat gagcgaactg cggacatcgt
     1021 ggagacaaca tgctgctggg tgcggatatc ccatggcacg tcatatcgct cgccgtatta
     1081 cttgttattt ttttgaaacg agaagttgat acaattcctt cagggataag gctaataact
     1141 cattcttcct gatgagatct ttatcagcgt ctataccctc tgcgcatgat tcatttaaat
     1201 agttcagtgt ttcctgatca tcatccaagc attcatagct gcctgataaa attcatcgca
     1261 acttgctttg ttatttttgt caatgtaacc aatgcattga atcacgcctt taacaacttt
     1321 agcagcagaa aaaacaaagt aactgcagtc atttatatag cgttcaatat caaaaatcat
     1381 ataaaaaata gtgcatagag tgtttttcaa tttcaaactc acttgaaatc atagctattt
     1441 cacattcaag gtctttcaga ccatctaaca ttatggaaag tttacattgt ttttcttgca
     1501 cagtttcata cttaaatgac tctctcccct ttgccctgac atttattctt tcttcaattg
     1561 cataaatata agcaggatat acacgcgacc acacttggtt ttatttttag gattaaccgg
     1621 cctctttcag cctttctttt gtagctataa attctgcata tagcgcagca tcctcttcgc
     1681 accatacatc aaacttttcg tttataaacc caactttaat tctttcccta cgcattcagg
     1741 aaacaaagga tcaaagtcct ttaagtattc aaactcactt agatagaatc ctgtctcata
     1801 acgattaaat aatttcctaa actctttccc acttaaggtt ttcattgtat caatcactct
     1861 gttttcataa aagcaaagtc aaacaagata aaacaataaa aactcttaca caaccagctg
     1921 atgtttttaa aatagaggat aatataagac aagcataacc tcgttcactt caatgctact
     1981 cctatcgttc ttttgcgtca cagagcgcct gccggacaac tgcatcaaga ccaacggaac
     2041 tgcttccgtc gattaatcag atggcagcgc ataaacacgc tcaacaaccc gtttgactgc
     2101 ttcaatttta agctcttcgg aataacgcta ccgtgtaagt ctccctgcaa aacgagctat
     2161 caacttttaa ggatctctgg cccccctgct tccgctcctg gcacacagca gccctagaga
     2221 cagtggagta aagtcatgga ggctcggtgg ggagaggtgc aaatcctccc atacaaaaaa
     2281 tacgaaaatc agtaacggct agaaatcatt caatacatgc actctcgaaa ttgcatcagc
     2341 caaccgcaaa acgttatttc aaagcctact agctgcctta cttttttcct caataacgcg
     2401 tcgccagccg gaaacaacct aatgccctgt tttggcctca aaccgctaat gataatcatt
     2461 ttcattttgt ataggttgtg ctatattaaa catagtagtt gctatgttta ttctaatttt
     2521 gctcactata ggtactaaat catgctctca ataaaaaaag taaccatgct cttggggtgc
     2581 ctagtcctca cctgctcgat agcatttcag gcgagtgcag ctgaaaaatt caaggtcatt
     2641 acaactttta ccatcatcgc agatatggcc aaaaacgtgg ctggagatgc tgcagaagtc
     2701 tcatccataa ccaagcctgg tgcagaaatt catgagtatc agcctacccc tggcgatatt
     2761 aaacgtgcgc agggggcaca actgattctc gccaatggta tgaatctgga attgtggttc
     2821 caacgctttt accagcatct caatggggtt ccagaagtaa ttgtctcttc gggtgtgacg
     2881 ccagtaggga tcaccgaagg gccctatgag ggcaaaccta acccccatgc ctggatgtcg
     2941 ccagataatg ctctgattta cgtcgataat attcgtgatg cgtttataaa atacgatccg
     3001 gcaaatgcac aaacctacca acgtaatgcc gatacttata aagccaagat tacccaaacc
     3061 cttgcccccc tgcgtaagca gattgcggaa ctccctgaga atcagcgatg gatggtcacc
     3121 agtgaagggg ctttttctta tctcgcacgc gatttggggc taaaagagct ttatctgtgg
     3181 ccgattaatg ccgatcaaca aggaacaccg cagcaggtac gtaaggttgt tgatatagtt
     3241 aagaaaaatc atatcccggc agtctttagc gagagtacga tttccgataa accagcgcgt
     3301 caggttgcgc gtgaaaccgg cgcgcactac gtggtgtgct ctatgtcgat tccctgagca
     3361 cagaaaacgg cccggtacct acgtatatcg accttctgaa ggttactacc agtacgctgg
     3421 tacagggaat taaagccgga aaaagggaga aataatgatg caatctgcag gcattgtcgt
     3481 taatgatgtt actgtcacct ggcgtaacgg gcacacagca ctgcgcgatg catccttcac
     3541 agtaccaggc ggatctattg ccgctctggt tggggtaaac ggttccggaa aatcgacgtt
     3601 gtttaaagcg attatgggat ttgtgcgtct gaccagcggg aagatatctg ttctgggtat
     3661 tcccacacga caggcgctac agaaaaacct ggttgcctac gtccctcagt cagaagaggt
     3721 tgactggtca tttcctgtac tggtggaaga tgtggtgatg atgggccgct atgggcatat
     3781 gggtatgttg cgtatcgcca aaaagaggga tcgtcagatt gtcaccgatg cgctggaacg
     3841 tgtcgatatg gtggattttc gccatcgaca aatcggcgag ctttccggtg gacaaaagaa
     3901 acgcgtcttt ctggcgagag cgattgcaca gcagggtgat gtgatcctgc tggatgaacc
     3961 ttttaccggc gttgatgtga aaaccgaagc gaaaatcatc agcctgttga gggagttacg
     4021 cgcggaaggc aaaacgatgc ttgtctcaac gcacaatctc ggatccgtca cgacattttg
     4081 cgattatacg gtcatggtca aaggcaccgt cctggcaagc gggccgacag acaccacttt
     4141 tacagccgag aacctggagc tggcttttag cggcgtactg cgccatgtca cacttaacgg
     4201 ctcggaagaa agcattatta ctgacgatga acgccctttt gtggcacatc gaccgtcagc
     4261 ggtgcagagg gaagaaagat gaacgcgcta ctggaaccct tcagctatga atatatgctc
     4321 aatgcgatgt gggtctcggc gatggtcggc ggtctctgcg ctttcctgtc atgctatctg
     4381 atgctaaaag gctggtcgct gattggcgat gcgctgtcgc actctattgt cccgggtgtt
     4441 gcgggggcat atatgctggg gcttccgttt tctctgggag ccttcttttc ggcggactgg
     4501 cgcgggcagc atgctgtttc aaccaactca ctcgcttaaa agaagatgcc atcatcggcc
     4561 tgatcttctc gtcctttttt ggcctggggc tgtttatggt gtcattgaac ccgacatccg
     4621 tgaacattca gaccatcgtc ctgggcaata ttctggcaat cgatccggcg gatatcctgc
     4681 aactcactat cattggcatt ctctcgatca tcgtgctttt tttcaaatgg aaagatctta
     4741 tggtgacctt tttcgacgag aatcacgccc gtgctattgg atgcatccgg gcagattaaa
     4801 aatcctcttc ttcaccctgc tgtcggtttc gaccgttgcg gcgctgcaaa cggttggtgc
     4861 ctttctggtg atctgtctgg tggtgactcc cggtgcaaca gcatggttgt taaccgatcg
     4921 ttttccccgc ctgctcatga ttgctgtaac tattggcagt gtgaccagtt tcctcggcgc
     4981 gtgggtcagt tactttctgg acggcgcgac aggtggaatc atcgtggtgg cgcagacact
     5041 gctgtttctt ttagcatttg tctttgcgcc cacccatggg cttttggcaa atcgtcggcg
     5101 tgcgcataaa gcgctggagg atcgctcatg atggcattat tactggagcc actgcagttc
     5161 acctttatga gtcacgcact gctgatttca ctggtagttt ccataccctg tgcgctgctg
     5221 tcggtctttc tggtcctgaa aagttgggca ctgatgggtg acgcgatgag tcatgcggtt
     5281 tttcctggca tcgtgcttgc ctggattctg gggttacctc tggctacagg ggcattcgtc
     5341 gccggagtat tctgtgcggt ggcgacggga tacctgaaag acaacagccg aatcaagcaa
     5401 gatacagtga tggggattgt tttttctggc atgtttgccg caggtctgat cttgtatatt
     5461 gcagtcaaac cagacgtaca tcttgatcat attcttttcg gcgacatgct cgggataacc
     5521 atcggcgata taatccagac ggtgattatt gccggactgg ttacacttgt tattagcgta
     5581 aaatggcggg attttttgct gttcagcttt gattatcaac aggcgcaggc aagcggtttg
     5641 catacgagat ggctgcatta tgggctgctg tgcatggtct ccctgaccat tgtggcgacg
     5701 ctgaaagcgg taggtatcat tctctctatt tctcttctca ttgcgcctgg tgccattgcg
     5761 gtactcctga cacaacgctt tcatattgcg ctcctgctgg cgactggcat atcgatattg
     5821 gtctcaatga ctggtgtctg gctgtccttt tttatcgaca gcgcgccagc tccgacaatt
     5881 gttgtcttat tcgcggtcat gttcatcatg acgtttaccg ttaccagcat caacgcacgc
     5941 acaaaggaaa atgccgaacc acgggatttg ttatcatccg attagcagaa cactcggcca
     6001 gcaacatccg cttattggcg atgcgcttga acgaataatg cacagactta tgattctgat
     6061 aagttttgtt gccagagagt tgctgcggac tggatagcaa gtaatgtcga ttctcagggg
     6121 tagcaaacag cgcagtaaaa tgagggaagt agtggcaaaa catagagaaa attggggggc
     6181 gagggccagt gatgtaaaaa accacgctaa gagtttaatt aattgtttgt attgaagttt
     6241 aatgatctta actcaccctt aagaatatct tccggtaacg aagacgtata atgccccagc
     6301 atatttatat ggctatgtat cagcagcgat aatcttgccg gatgctactc ctcaggagtt
     6361 tctcccgatt tgcgaagatg tgacaacgcc tcctgcatgt aaagtgtgtt tcacagcaca
     6421 accgcattgg tcaccagccc cagagcacct agttgatctt cctgccctta acggtagctg
     6481 aagatgactg ggctggatgg aaaatcctgc atggtgcatt gggagagcag atagaattgg
     6541 ttggcgacga tctgtttgtg accaacgtaa agtatattca gcgcggcatt gacgaaaacc
     6601 ttgccaattc agcattgatt aaactcaacc agattggttc cctgagtgaa acctttgatg
     6661 cggtacaact ttgccatgac aacaactggg gcacctttat ctcacaccgc agtggtgaga
     6721 cagtggacag ctttatcgcc gatatgacag ttgcaatgcg cgcaggtcat ctcaaaacgg
     6781 gagctccatg ccggggtgag cgtattgaaa aatataacca gcttatgaga attgaggatg
     6841 aactgggttc atcggcgcaa tttgccggaa agtctgcctt taaatgacat tattgtgcca
     6901 atcccccagg cttacacgtc ggggttattc tggaggaatg atgaaagctc acgtttttgt
     6961 tatttctatg cttactggca ttgtcgtcac ctatgccgtg ttacttcttg gttgcttgtt
     7021 catcgataaa acgctcccca ctgtagatgt tgtcattctg tcacttgtcg tgggggcttc
     7081 tgctcagcaa ttgtcgaggg tattaatgtc aataaataga accttccctt atcttgcatg
     7141 tagtcaaagg atataaatta acaatatcag tcaaatgaga acatattctg atattgttac
     7201 gataaaaaat tattttatga ggggtacatg attaaatcac tttttgccgt cattatcggc
     7261 ggttctgttg gatgtactct gcgctggtta ctgtccacac gctttaacag tctttttcca
     7321 aatctacctc caggtacact ggtggtgaac ctccttgccg ggttaattat tggtactgcg
     7381 ttggcgtact ttttacgaca accacacctt gatccttttt ggaaattaat gataacaacg
     7441 ggactttgtg gaggtctgag tactttctct acattttctg ttgaagtatt cgcattactc
     7501 caggcgggaa attatatatg ggctttgacg tctgtacttg ttcacgtcat tggctccctc
     7561 atcatgacag ctttaggctt ttttatcata accatacttt tcgcatgaga attcattaac
     7621 gttacaggtt tatcaactaa tgtgacgctt aggatagaat ttcgctccat ggtatgcgtt
     7681 tgcagtcatt acagtctcca tgacgactgt aatgactgca aacgcattac ctgacatatc
     7741 agtataaaca atgaattaac ctcttggcgt attttttaca tcactggccc tcacccccaa
     7801 aattctctct gaaaaattgt tgcggttaac gttgccaatg tatccaccca tttcagaaga
     7861 cgatctggca tcctggtggc atgctgcgat gtgcttcagg tgagtgcact gggcggtcag
     7921 cacaggcggt ttcgacagcg aaattgcaca ggacgttatg ccggctgtga gtacaacatc
     7981 atagccttac ttgattgtac ggtaatgcca aatacatgca gacactatgg taaataatac
     8041 agcggcaaat aatgccgtca caatcagcgc gattgagaca cctgttttcg acgccagtgg
     8101 caccaacaat gcgcacagcc catagccaag tgtgtgactg gccgcaagcc agcccgcgcc
     8161 tgcgccgtcg gccagcttat cattcagtaa aagttgataa gcgggtgttg ccagagcggc
     8221 cccaaatgac agcactgcac agcccaggta aaacaaccat agctgttcag tgagcatgat
     8281 agccagacca ctactcatca acaccccggc actcaggagc agagccactg gagtcaggcg
     8341 ctgtggacgg agtaccacga actgcgcgat aagcgcagct attgcggaca gccccaacaa
     8401 ccacgccacc tgttggctaa tagtggtggt atcagtatcg aactggcgag taagggctgg
     8461 cgaaagtcca agctgcatca tgctcattgc cgcagccagt aacattgcgc aaagcagata
     8521 aggcaggcaa tcccgcttca ggctgacgct cttgcgctcc ggtgtgggct gtggtggtgt
     8581 gccgggcaac cgcagaagca tcaccacagc ccacaccgga gctgccatca gcatccacac
     8641 tggcgccaga gggtgaatga ccaacattgc cgccgcgcac agcggcccga atagcctgcc
     8701 gcagctcagg ccggagctga tggttgccag agcggccatg cgattccctt ctcccgctct
     8761 ctgcaatgcc cagacctgac aggctggcac cattgctgac acggtcagac cgtagacgat
     8821 ccgggcgatg attaatattc ccaaccccac cgctgttgtt acggcaccgg tagccatcag
     8881 cacgcttccc agcccaagca gggtaaagct tacgccatat cctcccagag cccacagcac
     8941 cacagtctta ctgccagtaa gggaaatttg ctttccccac catggggaag atggcagaaa
     9001 cagcattgag cccagcatca gcagggcagc ccatacagac agactcagat ttgtctgtat
     9061 gaccagaaca gggagtacaa ccagcagacc gttctgtccg ataccgagaa gaccggcact
     9121 gaacgccagt ggccagcagg acagggtttt ttggggcgta tcttcgatct tagatgacat
     9181 tgtaatgttt caacaccatg tattaattgt gtttatttgt aaaattaatt tttctgacaa
     9241 taacatttct cattgataat gagaatcatt attgacataa ttgttattat tttactgtgt
     9301 aggagctgtt tgattatgat cctgccctct gaaaaatccg ccacagatgt ggctgcgcag
     9361 tgcttcctga atgccttgat tcgtgaaacc aaagactggc aactggcgga atacccgcca
     9421 gacgaattga ttatcccgct ggacgaacag aagtcgctcc atttcagagt ggcttatttc
     9481 tccccaaccc agcatcaccg ctttgcattt cctgctcatc tggtcacggc atcaggcagt
     9541 tatcctgtcg actttaccac tctctcccgg cttattattg ataagctccg gcatcaactt
     9601 tttctgccag ttcccctctg cgaaactttc caccaacgcg tgctggaaag ctacgcccat
     9661 acgcaacaga caattgatgc ccgccatgac tgggccatcc tgcgtgaaaa agcgttgaat
     9721 tttggcgagg ctgagcaggc actgctgaca ggacacgctt tccaccctgc acctaagtct
     9781 catgaaccgt ttaaccgcca ggaagctgaa cgatacctgc ctgacatggc acctcacttc
     9841 ccgctgcggt ggttttcggt ggataaaacg caaatcgctg gtgaaagtct gcatcttaac
     9901 cttcaacagc ggttgacgcg atttgccgca gaaaatgcgc cccagttact caacgaatta
     9961 agtgacaatc aatggctgtt cccgctgcac ccgtggcagg gagaatatct tttgcagcaa
    10021 gtgtggtgcc aggcactttt tgctaaagga cttatcagag acttaggcga ggccggcacg
    10081 tcgtggctgc cgaccacctc ttcccgctcc ctctactgtg ctaccagccg cgatatgatc
    10141 aagttctccc tgagcgttcg gctgaccaac tccgtccgta ctctgtctgt gaaagaggtg
    10201 gagcgaggaa tgcgcctggc acgtctggcg caaaccgacg gctggcagat gctacaggcc
    10261 cgcttcccta ctttccgggt aatgcaggag gacggctggg ccgggctgcg cgatcttaac
    10321 ggcaacatca tgcaggaaag tttgttttcc ctgcgtgaaa acctgctgct ggagcagccg
    10381 caaagccaga ccaacgttct ggtttccctg actcaggccg caccggatgg cggtgattcg
    10441 ctgctggttt cggcggtaaa acgcctgagc gatcgcctcg gtatcactgt gcaacaggcc
    10501 gcccacgcat gggtcgatgc ttactgtcag caggtgctga agcctctgtt tacggctgaa
    10561 gcggattacg gcctggtgct gctggcgcat cagcaaaata ttcttgtgca gatgcttggg
    10621 gatctgccgg tcggatttat ttaccgtgac tgccagggca gcgcgtttat gcctcacgcg
    10681 acagagtggc tcgataccat tgacgaggcg caggcggaaa atattttcac ccgtgagcag
    10741 ttgctgcgct atttccctta ttacctgctg gttaactcca cttttgccgt aactgctgcg
    10801 ctgggtgctg ccgggctgga cagcgaagcg aatctgatgg ctcgtgtacg aacattgctg
    10861 gctgaagtgc gtgaccaggt gactcataaa acctgtctca actatgttct ggaaagtccg
    10921 tactggaacg taaaaggtaa ctttttctgt tatctgaacg atcataacga gaacaccatc
    10981 gttgaccctt gatctatttt gatttcgcta acccgctgca ggctcaggag gtctgaatgt
    11041 ctggggcaaa cattgttcac agcggatatg gactgcgctg tgaaaaactc gacaagcctc
    11101 tgaatcttgg ctgggggctg gacaatagcg cggtgttgca ctggcccggg gagctgccaa
    11161 cagggtggct gtgcgacgcg ctggatcaga tatttatcgc cgcaccacaa ctttcagcag
    11221 tggttcttcc ctggtccgaa tggtatgagg agccacaggc gctgacgctt ttcggacagg
    11281 tacaaagcga cattatccat cgttccgctt tctggcagtt accgttatgg ctgagttctc
    11341 cggcaaaccg ggcctccggt gaaatggttt ttgatgcaga gcgtgagatt tatttcccgc
    11401 agcgcccccc ccgtccgcag ggtgaagttt atcgtcgtta cgatccacgc attcgcagga
    11461 tgctgagttt ccgcattgcc gatcccgttt ctgatgcaga acgtttcact cgctggatga
    11521 acgatccgcg cgttgagtat ttctggagca aagtggctca ctggaggtac agatcgccta
    11581 tctggaacgc cagctcacca gtaaacatgc attcccgctg attggttgtt tcgacgatcg
    11641 gccgttcagt tatttcgaaa tctactgggc ggcggaagac cgcattggtc gccactattc
    11701 gtggcagccc tttgaccgtg gcctgcatct gctggttggt gaacagcaat ggcgcggagc
    11761 ccactatgtg caaagctggc tgcgcggggt gacacattac ctgctgctga atgagccacg
    11821 tacgcagcgc acagtactgg agccacgcat cgataaccag cgcctgttcc gccatcttga
    11881 gcctgcggga taccggacaa ttaaagagtt cgacttccca cataaacgct cgcgcatggt
    11941 gatggcggat cgtcatcact tcttcacgga ggtcggtctg taatgaatca caaagactgg
    12001 gatttggtca accgccgcct ggtggcaaaa atgttgtctg agctggagta tgagcaggtt
    12061 ttccacgcgg aatctcaggg cgatgaccgc tactgcatta acctgccggg agcacaatgg
    12121 cgcttcatcg ctgaacgtgg tatctggggc tggctctgga ttgatgctca aactctgcgc
    12181 tgcgcggacg agccagtact ggctcagacg ctgctgatgc agctaaagca ggtactgtca
    12241 atgagcgatg caaccgttgc tgagcatatg caggatttgt atgccacgct gctgggcgac
    12301 ctgcaactac tgaaagcccg tcgcgggctg agcgccagtg acctgattaa tcttaatgcc
    12361 gaccgcctgc aatgcctgct gagcggtcat cctaaattcg tttttaataa aggtcgccgt
    12421 ggctggggta aagaggcgct ggaacgatat gcgccagagt atgccaacac cttcagactg
    12481 cactggctgg cggtaaaacg tgaacatatg atctggcgct gtgataacga gatggatatt
    12541 catcagttgt tgacggccgc aatggatccg caggagtttg cccgcttcag tcaggtctgg
    12601 caggaaaacg gactggatca taactggctg ccgctgccgg tacatccgtg gcagtggcag
    12661 caaaaaatcg ctaccgactt catcgctgat tttgccgaag gcaggatggt gtctctcggc
    12721 gagtttggcg accagtggct cgcccagcag tcgctgcgta ccctgaccaa cgccagtcgc
    12781 cggggcgggc tggatatcaa gctgccgctg accatctaca acacctcatg ctaccggggg
    12841 atccctggca gatacatcgc tgccggacca ctggcttcac gctggctaca acaggttttt
    12901 gcgaccgacg ccaccctagt gcaaagcggc gcagtgatac ttggtgaacc ggctgcaggc
    12961 tatgtctccc atgaaggcta tgccgcgctt gcccgggctc cctatcgcta ccaggaaatg
    13021 cttggtgtta tctggcggga gaatccgtgc cgctggctga aaccggatga aagtccggtt
    13081 ctgatggcaa cactgatgga gtgcgacgaa aacaatcagc cgctggcagg cgcatatata
    13141 gaccgctccg ggctggacgc tgaaacctgg cttacgcaac tgttccgggt ggtcgtggtt
    13201 cctctgtatc acctgctttg ccgctacggt gtcgcgctta ttgcacatgg acaaaatata
    13261 actctcgcca tgaaagaggg ggttccacag cgtgttctgc tgaaagactt ccagggcgat
    13321 atgcgactgg tgaaagaaga gttccccgaa atggactctt tgcctcagga ggttcgtgat
    13381 gttacatccc gcctgagtgc ggactactta atccatgatt tgcagacggg tcacttcgtg
    13441 acagtactgc gttttatttc gccactgatg gttcgtcttg gcgtacctga aaggcgattt
    13501 tatcaactgc tggcagcagt gttgagtgat tacatgaaaa aaacatccac aaatgtcaga
    13561 gcgttttgcg cttttctcac ttttcaggcc acaaatcatt cgcgtggtgc tgaacccggt
    13621 aaaactgacc tggcctgatc tggatggcgg cagccgcatg ctgccgaatt accttgagga
    13681 tctgcaaaat ccgctgtggc tggtaactca ggaatatgaa tcatgaaaaa aagtgtcgat
    13741 tttattggtg tagggacagg gccatttaat ctcagcattg ctgcgttgtc acatcagatc
    13801 gaagaactgg actgtctctt ctttgatgaa catcctcatt tttcctggca tccgggtatg
    13861 ctggtaccgg attgtcatat gcagaccgtc tttctgaaag atctggtcag tgctgttgca
    13921 cctacaaatc cctacagttt tgttaactat ctggtgaagc acaaaaagtt ctatcgcttc
    13981 cttacaagca gactacgtac agtatcccgt gaagagtttt ctgactacct ccgctgggct
    14041 gctgaagata tgaataacct gtatttcagt cataccgttg aaaacattga tttcgataaa
    14101 aaacgtcgat tgtttctggt gcaaaccagc cagggagaat attttgcccg caatatctgc
    14161 cttggtacag gaaaacaacc ttatttacca ccctgtgtga agcatatgac acaatcctgt
    14221 ttccatgcca gtgaaatgaa tcttcgtcgg ccggacctta gtggaaaacg gataaccgtg
    14281 gttggtggag gacagagtgg tgcagacctg ttccttaatg cattacgcgg ggaatgggga
    14341 gaagcggcgg aaataaactg ggtctcccgg cgtaataatt ttaacgcact ggatgaggct
    14401 gcttttgctg atgagtattt tacacctgaa tatatttcag gcttctccgg actggaggaa
    14461 gatattcgcc atcagttact ggatgagcag aaaatgacat cggatggcat cactgccgat
    14521 tctttactga ccatttatcg tgagttgtac caccgttttg aagttctgag aaaaccaaga
    14581 aatatccgtc tgctacccag ccgctcggta acaactctgg aaagtagtgg tcctggctgg
    14641 aagttgctga tggagcatca tctggatcag ggcagggaga gcctggaaag tgatgtggtg
    14701 attttcgcca caggttaccg ttctgcgttg ccacaaatac ttccctcact gatgcccctg
    14761 atcaccatgc acgataagaa cacctttaaa gtgcgtgatg acttcactct ggaatggagt
    14821 ggcccgaaag agaacaacat ctcg
//
