LOCUS       NZ_CP098148            12452 bp    DNA     linear   CON 25-JUN-2024
DEFINITION  Klebsiella pneumoniae strain Z0117KP0020 plasmid pZ0117KP0020-3,
            complete sequence.
ACCESSION   NZ_CP098148
VERSION     NZ_CP098148.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28703769
            Assembly: GCF_023657715.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Klebsiella pneumoniae, a One Health interface shuttle organism for
            the genes of CTX-M ESBL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12452)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2022) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul, Select One 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v10.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 260x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023657715.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 04:31:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,682
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,429
            CDSs (with protein)               :: 5,429
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 57 of 128
            Pseudo Genes (incomplete)         :: 71 of 128
            Pseudo Genes (internal stop)      :: 15 of 128
            Pseudo Genes (multiple problems)  :: 15 of 128
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP098148.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12452
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Z0117KP0020"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:573"
                     /plasmid="pZ0117KP0020-3"
                     /geo_loc_name="South Korea"
                     /collection_date="2017-08"
     gene            complement(<1..265)
                     /locus_tag="NBY48_RS28245"
                     /old_locus_tag="NBY48_28240"
                     /pseudo=""
     CDS             complement(<1..265)
                     /locus_tag="NBY48_RS28245"
                     /old_locus_tag="NBY48_28240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /name="IS6 family transposase"
     gene            <331..1845
                     /locus_tag="NBY48_RS28250"
                     /old_locus_tag="NBY48_28245"
                     /pseudo=""
     CDS             <331..1845
                     /locus_tag="NBY48_RS28250"
                     /old_locus_tag="NBY48_28245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /name="Tn3-like element TnAs1 family transposase"
     gene            1887..2129
                     /locus_tag="NBY48_RS28255"
                     /old_locus_tag="NBY48_28250"
     CDS             1887..2129
                     /locus_tag="NBY48_RS28255"
                     /old_locus_tag="NBY48_28250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032140899.1"
                     /translation="MRKAEREDGRERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="hypothetical protein"
     gene            complement(2161..2811)
                     /gene="tetR(A)"
                     /locus_tag="NBY48_RS28260"
                     /old_locus_tag="NBY48_28255"
     CDS             complement(2161..2811)
                     /gene="tetR(A)"
                     /locus_tag="NBY48_RS28260"
                     /old_locus_tag="NBY48_28255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            2917..4116
                     /gene="tet(A)"
                     /locus_tag="NBY48_RS28265"
                     /old_locus_tag="NBY48_28260"
     ARG             2918..4113
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(4148..5031)
                     /locus_tag="NBY48_RS28270"
                     /old_locus_tag="NBY48_28265"
                     /pseudo=""
     CDS             complement(4148..5031)
                     /locus_tag="NBY48_RS28270"
                     /old_locus_tag="NBY48_28265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /name="EamA family transporter"
     gene            complement(5169..5561)
                     /locus_tag="NBY48_RS28275"
                     /old_locus_tag="NBY48_28270"
     CDS             complement(5169..5561)
                     /locus_tag="NBY48_RS28275"
                     /old_locus_tag="NBY48_28270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="WP_001351729.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIER
                     GDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRLVQ
                     DCCYDPDRDAHEALLRSGFGGRVQVV"
                     /name="isochorismatase family cysteine hydrolase"
     gene            <5565..>6314
                     /locus_tag="NBY48_RS28280"
                     /old_locus_tag="NBY48_28275"
                     /pseudo=""
     CDS             <5565..>6314
                     /locus_tag="NBY48_RS28280"
                     /old_locus_tag="NBY48_28275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            complement(6338..6943)
                     /locus_tag="NBY48_RS28285"
                     /old_locus_tag="NBY48_28280"
     CDS             complement(6338..6943)
                     /locus_tag="NBY48_RS28285"
                     /old_locus_tag="NBY48_28280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509965.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGCAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            7038..9933
                     /locus_tag="NBY48_RS28290"
                     /old_locus_tag="NBY48_28285"
                     /pseudo=""
     CDS             7038..9933
                     /locus_tag="NBY48_RS28290"
                     /old_locus_tag="NBY48_28285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            <10058..11769
                     /locus_tag="NBY48_RS28295"
                     /old_locus_tag="NBY48_28290"
                     /pseudo=""
     CDS             <10058..11769
                     /locus_tag="NBY48_RS28295"
                     /old_locus_tag="NBY48_28290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490160.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element IS3000 family transposase"
                     /name="Tn3-like element IS3000 family transposase"
     gene            <11844..12245
                     /locus_tag="NBY48_RS28300"
                     /old_locus_tag="NBY48_28295"
                     /pseudo=""
     CDS             <11844..12245
                     /locus_tag="NBY48_RS28300"
                     /old_locus_tag="NBY48_28295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            12342..>12452
                     /locus_tag="NBY48_RS28305"
                     /old_locus_tag="NBY48_28300"
                     /pseudo=""
     CDS             12342..>12452
                     /locus_tag="NBY48_RS28305"
                     /old_locus_tag="NBY48_28300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="fluoroquinolone resistance protein"
                     /name="fluoroquinolone resistance protein"
     ARG             12343..12452
                     /name="QnrB30"
                     /gene="QnrB30"
                     /gene_name="quinolone resistance protein (qnr)"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="100.0"
                     /coverage="16.37"
ORIGIN
        1 gcccagcggc cattgacctt cacgtaggtt tcatccatgt gccacgggca aagatcggaa
       61 gggttacgcc agtaccagcg cagccgtttt ccatttcagg cgcataacgc tgaacccagc
      121 ggtaaatcgt ggagtgatcg acattcactc cgcgttcagc cagcatctcc tgcagctcac
      181 ggtaactgat gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa
      241 aatgccggcc tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt
      301 atcaccaccg actatttgca acagtgccac ttcgacgact acctgctgcc ggccgagaag
      361 ttcgccgcac tcaagcgcga gcaggccctg cccctggcga tcaacccgaa cagcgaccag
      421 tacctggaag agcgtttgca gctgctggac gagcagttgg ccaccgtcac ccgcctggcc
      481 aaggacaacg agctgcccga tgccatcctc accgagtcag ggctgaaaat caccccgctg
      541 gatgcggcgg tgccggatcg ggcgcaggcg ctgatcgacc aaaccagcca gttactgccg
      601 cgcatcaaga tcaccgaact gctgatggac gtggacgact ggacgggctt cagccgccac
      661 ttcacccact tgaaggacgg ggccgaggcc aaagacagga cgttgctgct gtccgcaatc
      721 ctcggtgatg cgatcaacct cgggctgacc aagatggccg agtcgagccc cggcctgacc
      781 tacgccaagc tgtcctggct gcaagcctgg cacatccgcg acgaaaccta ttcggcggcc
      841 ttggccgagc tggtcaacca ccagtatcgc cacgcctttg ccgcccactg gggcgacggc
      901 acgacctcat cctccgatgg ccagcgcttc cgcgcgggtg gccggggcga gagcaccggg
      961 cacgtcaacc cgaagtacgg tagcgagccg ggacggctgt tctataccca tatctccgac
     1021 cagtacgcgc cgttcagcac ccgcgtggtg aatgtcggcg tccgcgattc cacctatgtg
     1081 ctcgacggcc tgctgtacca cgagtccgac ctgcggatcg aggagcacta caccgacacg
     1141 gccggcttca ccgatcacgt ctttgccctg atgcacctgc taggcttccg cttcgcgccg
     1201 cgcatccgcg acctcggcga aaccaagctg tacgtgccgc agggcgtgca agcctacccg
     1261 acgttgcgcc cgctgatcgg cggcaccctg aacatcaagc acgtgcgtgc ccactgggac
     1321 gacatcctgc gcctggccag ctcgatcaag cagggcaccg tcaccgcctc gctgatgctg
     1381 cgcaagctcg gcagctaccc gcgccagaac ggactggccg tggccctgcg cgagctgggc
     1441 cggatcgagc gcacgctgtt catcctggac tggctgcaaa gtgttgaact gcgccgccgc
     1501 gtgcatgccg gcctgaacaa aggtgaggcg cgcaactcgc tggccagggc ggtgttcttc
     1561 aaccgccttg gggaaatcag ggatcggagc ttcgagcagc agcgctaccg ggccagcggc
     1621 ctcaacctgg tgacggcggc tatcgtgctg tggaacacgg tgtacctgga acgcgccacc
     1681 caggggttgg tcgaggccgg caagccggtg gacggcgagc tgctgcaatt cctgtcgccg
     1741 ctgggctggg agcacatcaa cctaaccggc gattacgtct ggcggcagag ccgcagactg
     1801 gaagacggga agtttcggcc cttacggatg cccggaaaac cttagcgtac gattttttcc
     1861 gaattctgcg ggctccccta tctcatctgc gcaaggcaga acgtgaagac ggccgcgagc
     1921 gccaggcgca cgaggccggc gcgcggaccc gcgccacggc ccacgagcgg acgccgcagc
     1981 aggagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     2041 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     2101 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     2161 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     2221 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     2281 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     2341 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     2401 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     2461 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     2521 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     2581 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     2641 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     2701 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     2761 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     2821 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2881 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacag acccctgatc
     2941 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     3001 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     3061 ctggcgctgt atgcgttgat gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     3121 cgtttcgggc ggcggccggt cttgctcgtc tcgctggccg gcgctgctgt cgactacgcc
     3181 atcatggcga cggcgccttt cctttgggtt ctctatatcg ggcggatcgt ggccggcatc
     3241 accggggcga ctggggcggt agccggcgct tatattgccg atatcactga tggcgatgag
     3301 cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt tcgggatggt cgcgggacct
     3361 gtgctcggtg ggctgatggg cggtttctcc ccccacgctc cgttcttcgc cgcggcagcc
     3421 ttgaacggcc tcaatttcct gacgggctgt ttccttttgc cggagtcgca caaaggcgaa
     3481 cgccggccgt tacgccggga ggctctcaac ccgctcgctt cgttccggtg ggcccggggc
     3541 atgaccgtcg tcgccgccct gatggcggtc ttcttcatca tgcaacttgt cggacaggtg
     3601 ccggccgcgc tttgggtcat tttcggcgag gatcgctttc actgggacgc gaccacgatc
     3661 ggcatttcgc ttgccgcatt tggcattctg cattcactcg cccaggcaat gatcaccggc
     3721 cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat tgccgacggc
     3781 acaggctaca tcctgcttgc cttcgcgaca cggggatgga tggcgttccc gatcatggtc
     3841 ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag caatgttgtc caggcaggtg
     3901 gatgaggaac gtcaggggca gctgcaaggc tcactggcgg cgctcaccag cctgacctcg
     3961 atcgtcggac ccctcctctt cacggcgatc tatgcggctt ctataacaac gtggaacggg
     4021 tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct gcgtcgcggg
     4081 ctttggagcg gcgcagggca acgagccgat cgctgatcgt ggaaacgata ggcctatgcc
     4141 atgcgggtca aggcgacttc cggcaagcta tacgcgccct aggagtgcgg ttggaacgtt
     4201 ggcccagcca gatactcccg atcacgagca ggacgccgat gatttgaagc gcactcagcg
     4261 tctgatccaa gaacaaccat cctagcaaca cggcggtccc gggctgagaa agcccagtaa
     4321 ggaaacaact gtaggttcga gtcgcgagat cccccggaac caaaggaagt aggttaaacc
     4381 cgctccgatc aggccgagcc acgccaggcc gagaacattg gttcctgtag gcatcgggat
     4441 tggcggatca aacactaaag ctactggaac gagcagaagt cctccggccg ccagttgcca
     4501 ggcggtaaag gtgagcagag gcacgggagg ttgccacttg cgggtcagca cggttccgaa
     4561 cgccatggaa accgcccccg ccaggcccgc tgcgacgccg acaggatcta gcgctgcgtt
     4621 tggtgtcaac accaacagcg ccacgcccgc agttccgcaa atagccccca ggaccgccat
     4681 caatcgtatc gggctaccta gcagagcggc agagatgaac acgaccatca gcggctgcac
     4741 agcgcctacc gtcgccgcga ccccgcccgg caggcggtag accgaaataa acaacaagct
     4801 ccagaatagc gaaatattaa gtgcgccgag gatgaagatg cgcatccacc agattcccgt
     4861 tggaatctgt cggacgatca tcacgagcaa taaacccgcc ggcaacgccc gcagcatcgc
     4921 gaccgtcatc ggtgagaagt tcggcaggta ttgggtggtg acaatgtagg tgctgcccca
     4981 aatggcaggt gctatcgctg tgaacaataa atcgggcgtg cgtaatgaca tgcgattaca
     5041 agacctccgc tacggcgatg ggtttttgag agttgcaggc gccgcgatga ccgcaccctg
     5101 acttggactg gccctgcctc gcaccaatcc acctgcggct atggccgcga tcatgcgagg
     5161 cccgaaaatt acacgacctg tacacgtccg ccgaacccgg aacgcaacag agcttcgtgg
     5221 gcatcccgat ccgggtcgta gcagcagtcc tggaccaaac gcacgtcgta gtccgcatca
     5281 ctagcccagg cgacgcttga aagaacaacg ccggtggtgc ttatcccggc catgacaagc
     5341 gtgcttacgc cgcgcgtccg aaggtcggcg tcgagcgccg tgccataaaa gacgctggct
     5401 cgcggacatg catagaaaag gtcgccccgt tcgatcgcaa gcccttcgac gggcagaccg
     5461 gtgcgaaatc gtccgctcgg gagatacggt gagatttggc gattcgtcgc cggcggcgca
     5521 tgttcatact cttcgccgag cgaaaagttg gggaacagca cgggccggcc gcgcaaggcg
     5581 tgctggcagc cgtgcagacc ctgcgtgaga tgaacgccga caacctgcgc aaggtgccgg
     5641 ccgatgcacc cacggccttc atcaagccgc gctggaagcc gctggtgatc accccggaag
     5701 gcctcgaccg gaaattctac gaaatctgcg ccctgtccga gctgaagaac gccctgcgct
     5761 ccggcgacat ctgggtcaag ggctcgcggc agttccgcga cttcgacgac tacctgctgc
     5821 cggccgagaa gttcgccgca ctcaagcgcg agcaggccct gcccctggcg atcaacccga
     5881 acagcgacca gtacctggaa gagcgtttgc agctgctgga cgagcagttg gccaccgtca
     5941 cccgcctggc caaggacaac gagctgcccg atgccatcct caccgagtca gggctgaaaa
     6001 tcaccccgct ggatgcggcg gtgccggatc gggcgcaggc gctgatcgac caaaccagcc
     6061 agttactgcc gcgcatcaag atcaccgaac tgctgatgga cgtggacgac tggacgggct
     6121 tcagccgcca cttcacccac ttgaaggacg gggccgaggc caaagacagg acgttgctgc
     6181 tgtccgcaat cctcggtgat gcgatcaacc tcgggctgac caagatggcc gagtcgagcc
     6241 ccggcctgac ctacgccaag ctgtcctggc tgcaagcctg gcacatccgc gacgaaacct
     6301 attcggggtc ggttccggct gagggcgaaa tgacacccta agcgttagct ctgtgtcgtt
     6361 gcacgatgtc agcgacggta ttcttgctga taccgagctc gcgtgcgatc cagcgatagc
     6421 tgcgtccctc ggccctcatc gcaaccacct taggcaaaag tcggtctgat tttggtcgca
     6481 ctccggcctg acgaccaagc ctcttaccac gtgccttcgc aacagcaagg cctgacttga
     6541 cccgctcgct gatgagatcc cgctcaaact ccgcaatgcc ggaaagaaac gtcgccagca
     6601 ttcgtccata cggcgacgaa agatcgaacg ccattccatt catggctatc acggaaacct
     6661 tccagttctc cagttcacgt agcgtattga gcagatcgag cgtcgagcgc ccccaccggg
     6721 aaagctcagt gaccaggatt gcatcaattt gtctggactg ggcaagcgcc aggactttct
     6781 ttcgctcggc ccggtcgagt ttagttcctg aacctgtttc cttaaatatt cccaccacgt
     6841 cgtagccggc acagccggcg aaggctcgca gatcaaattc ctggcgttca caagactgat
     6901 ccgctgttga aacccggcag taaatggcgg cacgatgtcc caattgaacc ctcctggatt
     6961 tttgtatcgg aacgccctga tttatatggg ctggctgttg tccaaaacag actatacttc
     7021 aaaagggacg aatttgtatg tcacgacgcc atattttcac cgaacggcag cgagcagcgc
     7081 tgttcgatct gcccacggac gaactgtcgc tactgaagtt ctacacgctg ggcgatgatg
     7141 acctggaaaa cattaggcag cgccgcagac cggaaaacag gattggcttt gccctgcaac
     7201 tttgtgcctt acgatatccg ggccgtgcac tggctcctgg tgagatgatc ccgcgtgaag
     7261 tcctttcctt cgtcggtgct cagcttggag ttccggctga tgcgcttctc acttatgcca
     7321 cacggcgcca aacccgtcag cagcacatgg acacgctgcg cgaaatttac ggctacaaga
     7381 ccttcacggg ccgtggtgcc cgtgatctgc gggagtggac tttcggccag gccgaagatg
     7441 ccagatcaaa cgaggatctt gctcatcgtt ttattgtgcg gtgtcgggaa acttccacca
     7501 ttctgcccgc agtatcgaca atcgagcgct tgtgcgcgga tgctggtcgc cgctgagcgg
     7561 cggattgaaa cgcggattgc ggaaaattta acagcggatg ttcgcgatca cctggacaaa
     7621 cttctgagtg aaatgctcgc cggcaatatc agtcgtttca tctggcttcg caacttcgag
     7681 gttggtaaca actcggctgc tgctaaccgt ttgctcgaca ggctcgaatt ctgcgtaccc
     7741 tgaatatcaa tcatagtgct ttggccagca tacctgccca tcgcattgcc cggctgcgtc
     7801 ggcagggtga acgctacttc accgacggtt tgcgtgacat cacttcggac cgccgctggg
     7861 cgatccttgc cgtctgtgtt gtggagtggg aagcggcgat tgctgatgcc atagtcgaaa
     7921 cccatgacag gatcgtagga aaaacctggc gggaagcgaa gcgccagcat gacgaaacaa
     7981 tttccggctc taaagccaca ctcacggata cgatccgtac cttcaccgcg ctgggagctt
     8041 cgttgcttga ggcccgcagt gacggaaccc cgctggagat ggctgtcgcc agttcggttg
     8101 catgggaccg gctcgctcaa ctggtagcga cagggactca actcagcaac acgctagccg
     8161 atgagcctct tgcatatgtc gggcagggat accatcgctt tcgtcgttat gcgccccgca
     8221 tgttgcgctg tctgaagctc gaagccgcgc cggtcgccgg accattggta gcagcagctt
     8281 tgtcgatcgg agagatgaaa ggtgttgcat cgccagaaag gcgtttcctg cggcccagct
     8341 ccaaatggaa ccgtcattta cgagctcagg aaaaaggaga tacccgtctt tgggaagtgg
     8401 cggtactctt tcacctccgg gatgcttttc gttccggaga tgtctggctc gctcattcgc
     8461 gccgctatgg tgacctcaag caggtactgg tgccgatgat cgcggcgcag gaaaatgcaa
     8521 aactggccgt gccttccaac ccacaggatt ggctggcaga cagaaaggcg cgactcacga
     8581 tcgctcttaa gcggctggcc cgggctgccc gtaacggcac tattccgcac ggtagcatag
     8641 aagatggaac gttgcggatc gacaggttga cagcagacgt gccggatggt gccgaggcac
     8701 tcatactgga tctgtatcgc cgaatgccgt ccgttcggat taccgacatg ctgcttgaag
     8761 ttgatgcagc ccttggtttc acagatgcgt ttacccatct gagaaccggg gctccatgtc
     8821 gcgaccggat cggtctgctc aacgtcctgc tcgctgaagg gctcaatctg ggcctgcgta
     8881 agatggcgga agctacaaac acgcatgatt actggcagct ctcacgcctt gcccgctggc
     8941 atgttgaaag cgaagccatg aaccaggcat tggcaattgt ggtggccgcg cagggtaaac
     9001 tgccgatgtc acgcgtctgg gggatgggca cgtcagcatc gagcgatggt cagtttttcc
     9061 cgacagcgcg gcatggcgaa gccatgaaca tggtcaatgc caaatatggt tctgttcccg
     9121 gcctcaaagc gtatactcac gtaagcgacc agttcgcgcc attcgcttgt cagtcgatcc
     9181 cggcgaccgt gagcgaggca ccgtatattc tcgatggact actgatgaac gaggtcggtc
     9241 gccatgttcg cgaacagtat gccgatacag caggattcac cgaccatttg ttcggagcca
     9301 gtagcctgct cggctacaat ctcgttctgc gaatcaggga tctgccatcg aagcggttgt
     9361 acgtatttaa tcccgatacg acccccaggg agttacgcaa gttggtaggt ggaaaagccc
     9421 gggaggatct tatcgttgcg aactggcctg atattttccg ttgtgccgcg acgatgaccg
     9481 ctggcaaaat caggcccagc caactcctgc gcaagctcgc ttcttaccca cgacaaaaca
     9541 accttgcagt tgcgcttcgt gaagttggtc gtattgaacg gacccttttc attattgagt
     9601 ggatcctgga tacggacatg cagcggcgtg ctcagatcgg tcttaacaag ggagaggccc
     9661 accatgcgct caaaaaatgc gctccgtatc gggaggcagg gggaaattcg cgatcgcacg
     9721 acagaggggc agcactaccg aatcgctggg ctcaatttat tgactgcggt gatcatttac
     9781 tggaataccg tccatcttgg tcatgccgtc acggagcggc ggaacgaagg gttggatgtt
     9841 ccccctgaat ttcttcccca catatcccca ttgggctggg cgcacattct actgactggc
     9901 gaatatcttt ggcccaagga accgaaagct tagggtgtca tttcgccctc agccggaacc
     9961 gaccccatgt ctgcgcttcg aaggctgcga cggcacccag cacttagcgg ccgccttggc
    10021 gaaaacacaa aacgaactca acggccagga cgctcagttg caaactgccg acaccagact
    10081 cctttccaaa aaatcacgtg aattcctgct ggatggtgaa gggaatatcc tgatcgatcg
    10141 ttatgagtgg ttcctctatc aacagattcc tgatcgcctg aatggccagc tgacgctgcc
    10201 tgatatcact aaataccggg cactcgacgc cgacctgatc gacggggaac attggcgaaa
    10261 aaacaaatat acgctgcttc aacagagcca ttttacaaaa ttagcggagg agcctgaaaa
    10321 gctgatcaaa cagatggcca tggaattgga tacccgtttg tacgaagtcg gcgaatatct
    10381 tgaacaggaa gacaaccgga atatcatctt gcgtaatccg cagggtaaac gcttctggct
    10441 gctgccttcg gccagcaaac atcatctggt caacaatccc ttcttccagc aaattcccac
    10501 aacggggatt gcggatgtac tgcgcatggt tgatcgtgac accggtttca ttgactgctt
    10561 cgctcatgtg ctgggttccc aatccagaag ccgttcccat gaatatgacc tgttggcaat
    10621 tctggtcggc aatgcaacca atcaaggcat ttacggcatg gcacagatct ctgatcgtac
    10681 ctatgatcag ctcagcacta tccaggcgaa ctatcttcgc ctggaaacat tgaatgctgc
    10741 taacgacaac atcaataacg cgacagccaa gctacccatc ttccggtact acaacatcca
    10801 ggaagatgtg atccacgcca gtgccgatgg tcaaaagttc gaagcccggc gcgagacctt
    10861 caaaacccgt tattcgtcga agtactttgg cactcaaaag gtgtttctgc catgaccttg
    10921 atcgccaatc acgctgcgat caacgccaga gtgatcggcg ccaacgaaca tgaatcgcac
    10981 tacatctttg atttgttgat gagcaatacg tcagacatca ttccggatgt gctctcaacc
    11041 gatacccatg gggtgaacca tgtgaacttc gcgttactgg atctgttcgg ataccagttt
    11101 gccccacgct atgcccaggt tggcaaagtg atcaatgaca tgtttgatgt caaggaagac
    11161 aaagaacacc gaattcagct gtgcttaaaa aagccaatca atacccatcg tattgcgcag
    11221 cactgggata ccatccaacg gattgcagta tcacttaagc agcggaaaac aacgcaagcc
    11281 accttggtga gaaagctctc ggagtacaag cgcaatcacc cgctgctgga agccctgacg
    11341 gaatacaatc gcctggtgaa agcgaattat ctactgtgct acatcgatga tgccagttta
    11401 agaaactatg ttcagcgcgc gctgaaccgg ggagaggcct atcaccaact gcgtcgggcc
    11461 gtgagcagcg tcaatgggga tcagttccgg ggcagttcag acgaagaaat ccagctatgg
    11521 aatgagtgcg ctcgcctggt caccaatgcc atcgtttact tcaactccag gatactcagc
    11581 cagctgttga ccagcttcga ataccaagga gataccaaga gaatagatat cgtcaaacag
    11641 gcatcccctg tggcctggca caacattaac ctcaagggga cttaccactt cgaattgagc
    11701 gaaaaattgc cagatctgga ggagcttatg cgctcaatcg agggatattt acccgtcagc
    11761 gaaaagtaat acccctctgg aggccacgga cggcgcggcc tccagagcac tttgtcgttt
    11821 ttggacggaa aatcctagaa ccccattttg ccattcagat gtgccgcgag atcggattac
    11881 ctctgtttcc gggttttagt gccgaggcca gagaaactct cctccactac cgttggccgg
    11941 gcaatattcg tgaactgaaa aacgtcgtgg agcgatccgt ttaccggcat ggaaccagtg
    12001 attacccgct ggatgagata atcattgacc ccttccgccg ccacactact cagccgcagg
    12061 cgccggaaac taaaccgatg tctgtagggt taccgctgga tctgcgtgaa ttccagcagc
    12121 agcaagagaa agactttcta caaaccagct tacagcaggc aaaatttaac cagaaaaaag
    12181 ccgctgaatt actgggtctg acttaccatc agcttcgcgc tttgctgaaa aaacaccaga
    12241 tttaacgcac atttgcagat gtcatattgg cggatttgac gcataaccac ctcatcaggg
    12301 tttaccatga cgccattact gtataaaaaa caggtacaaa tatggctctg gcactcgttg
    12361 gcgaaaaaat tgacagaaac cgtttcaccg gtgagaaaat tgaaaatagt acatttttta
    12421 actgtgattt ttcaggtgcc gacctgagcg gc
//
