LOCUS       NZ_CP098125             9250 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Klebsiella pneumoniae strain Z0117KP0042 plasmid pZ0117KP0042-2,
            complete sequence.
ACCESSION   NZ_CP098125
VERSION     NZ_CP098125.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28703774
            Assembly: GCF_023657615.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9250)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Klebsiella pneumoniae, a One Health interface shuttle organism for
            the genes of CTX-M ESBL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9250)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2022) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul, Select One 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v10.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 169x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023657615.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 04:19:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,309
            Genes (coding)                    :: 5,169
            CDSs (with protein)               :: 5,169
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 59 of 140
            Pseudo Genes (incomplete)         :: 78 of 140
            Pseudo Genes (internal stop)      :: 21 of 140
            Pseudo Genes (multiple problems)  :: 16 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP098125.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9250
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Z0117KP0042"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:573"
                     /plasmid="pZ0117KP0042-2"
                     /geo_loc_name="South Korea"
                     /collection_date="2017-11"
     gene            complement(join(9035..9250,1..753))
                     /locus_tag="NBY58_RS26945"
                     /old_locus_tag="NBY58_26930"
     CDS             complement(join(9035..9250,1..753))
                     /locus_tag="NBY58_RS26945"
                     /old_locus_tag="NBY58_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_013815101.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFTLMNVPLR
                     CPDYTSVSKRAKSVNVSFKTFTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRK
                     LHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRAASADGAYDTRLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETAMYRVK
                     QLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIT"
                     /name="IS5 family transposase"
     gene            complement(1041..1199)
                     /locus_tag="NBY58_RS27190"
     CDS             complement(1041..1199)
                     /locus_tag="NBY58_RS27190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(1854..2045)
                     /locus_tag="NBY58_RS26950"
                     /old_locus_tag="NBY58_26935"
     CDS             complement(1854..2045)
                     /locus_tag="NBY58_RS26950"
                     /old_locus_tag="NBY58_26935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165990.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGGDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2109..2430
                     /locus_tag="NBY58_RS26955"
                     /old_locus_tag="NBY58_26940"
                     /pseudo=""
     CDS             2109..2430
                     /locus_tag="NBY58_RS26955"
                     /old_locus_tag="NBY58_26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2436..2732
                     /locus_tag="NBY58_RS26960"
                     /old_locus_tag="NBY58_26945"
     CDS             2436..2732
                     /locus_tag="NBY58_RS26960"
                     /old_locus_tag="NBY58_26945"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_250838473.1"
                     /translation="MPGEKAGSGPVDYLLGRERNREGATVLQGDPEEVRELIDATPFAK
                     KYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKDGLS"
                     /name="hypothetical protein"
     gene            3106..3623
                     /locus_tag="NBY58_RS26965"
                     /old_locus_tag="NBY58_26950"
                     /pseudo=""
     CDS             3106..3623
                     /locus_tag="NBY58_RS26965"
                     /old_locus_tag="NBY58_26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /name="MbeB family mobilization protein"
     gene            3630..3864
                     /locus_tag="NBY58_RS26970"
                     /old_locus_tag="NBY58_26955"
                     /pseudo=""
     CDS             3630..3864
                     /locus_tag="NBY58_RS26970"
                     /old_locus_tag="NBY58_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4197..4493
                     /locus_tag="NBY58_RS26975"
                     /old_locus_tag="NBY58_26960"
     CDS             4197..4493
                     /locus_tag="NBY58_RS26975"
                     /old_locus_tag="NBY58_26960"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_250838475.1"
                     /translation="MTDKTTDIKMALENMIRFRDTIERLGGSMAKEFDVETKGKRTYSM
                     NNDMSDLYNVISRAVEASGADHSINEKLTNVLKRELVDYVRHCASKNQIVCII"
                     /name="hypothetical protein"
     gene            4510..4689
                     /locus_tag="NBY58_RS26980"
                     /old_locus_tag="NBY58_26965"
     CDS             4510..4689
                     /locus_tag="NBY58_RS26980"
                     /old_locus_tag="NBY58_26965"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_250838476.1"
                     /translation="MQLIAEYKEEIKFASSLQEDLRKERAKFFSETLKEVHQTLNESQV
                     DNEVASKWIKELDV"
                     /name="hypothetical protein"
     gene            complement(4853..6019)
                     /locus_tag="NBY58_RS26985"
                     /old_locus_tag="NBY58_26970"
                     /pseudo=""
     CDS             complement(4853..6019)
                     /locus_tag="NBY58_RS26985"
                     /old_locus_tag="NBY58_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(5986..6210)
                     /locus_tag="NBY58_RS26990"
                     /old_locus_tag="NBY58_26975"
     CDS             complement(5986..6210)
                     /locus_tag="NBY58_RS26990"
                     /old_locus_tag="NBY58_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_250838471.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVTTSIL
                     KRRGDTAALTLLQDTGFNPSSLSVYSPAL"
                     /name="hypothetical protein"
     gene            complement(<6324..6622)
                     /locus_tag="NBY58_RS26995"
                     /old_locus_tag="NBY58_26980"
                     /pseudo=""
     CDS             complement(<6324..6622)
                     /locus_tag="NBY58_RS26995"
                     /old_locus_tag="NBY58_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224844.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(6659..7489)
                     /locus_tag="NBY58_RS27000"
                     /old_locus_tag="NBY58_26985"
     CDS             complement(6659..7489)
                     /locus_tag="NBY58_RS27000"
                     /old_locus_tag="NBY58_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /GO_process="GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="WP_250838472.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYIWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAAFLRRLHSIPVCNCPFNSDHVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFHKYGIDNPDMNKLQFHLMLDEFFLIRIG"
                     /name="APH(3')-I family aminoglycoside
                     O-phosphotransferase"
     gene            7691..8716
                     /locus_tag="NBY58_RS27005"
                     /old_locus_tag="NBY58_26990"
     CDS             7691..8716
                     /locus_tag="NBY58_RS27005"
                     /old_locus_tag="NBY58_26990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632466.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="WP_001101446.1"
                     /translation="MNVKTIGIDLAKNVFQIHGVDEHGKRLFNKQLRRAQMASFFANIP
                     PCLIGMEACASAHFWANKLISMGHNVKLMAPQFVKPYVKTNKHDAADAEAICEAVTRPN
                     MRFVPVKTAEQQAVLALHRSRQSFIKQRTAQANQIRGLLAEFGIVVPRGIQQLQRRLPE
                     LVEDADNPLPVLFRTQLSLLQHHMAYLFDVIATLDKQIEQCYRQNALCQRIGKIPGIGP
                     VTASALIATIGKANNFENGRQLAAWLGLVPRQHSSGGKQVLLGISKRGDTYLRTLLIHG
                     ARAVLQSAKHKQDAVSSWANQLMARRNNNIASVALANKNARTVWALLAKEREYCAPIIS
                     A"
                     /name="IS110 family transposase"
     ARG             complement(6678..7489)
                     /name="aph(3')-Ia"
                     /gene="aph(3')-Ia"
                     /gene_name="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.52"
                     /coverage="100.0"
     ARG             complement(6660..7489)
                     /name="APH(3')-Ia"
                     /gene="APH(3')-Ia"
                     /gene_name="APH(3')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.79"
                     /coverage="101.85"
     Typing          1693..1773
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="100.0"
                     /evalue="2.24e-33"
     Typing          complement(1128..1669)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.526"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2443..2750
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.32"
                     /coverage="95.0"
                     /evalue="8.99e-51"
ORIGIN
        1 attagccact gcacggttac ggtctgcata ttcaccgggc cagtaacccg caccttttcg
       61 gggtgggata agcgcgctga ttttcttacg ccgcagttca tcgtgacaga gccgggtgtc
      121 gtaagcgccg tctgccgatg ctgccctgat ttttctgtga gtctgccgga taagacccgg
      181 gaaggcttct gagtccgtca cattgttcag cgacaggtca gcgcagatga tttcatgtgt
      241 tttactgtca acggcgagat gcagcttacg ccagatacgg cggcgttcct ggccatgctt
      301 tttgactttc cactcgcctt caccgaagac cttcagcccg gtggaatcaa tcaccagatg
      361 cgcgatttca ccccgggtga acgttttgaa actgacatta accgactttg cgcgcttgct
      421 gacactggtg taatccgggc agcgcaacgg aacattcatc agtgtaaaaa tggaatcaat
      481 aaaaccctgt gcagcccgca gggtcaacct gaacacgcgt ttaatgacca gcacagtcgt
      541 gatggcaagg tcagaatagc gctgaggtct gcctcgtgaa gaaggtgttg ctgactcata
      601 ccaggcctga atagcttcat catccagcca gaaagttatg gagccacggt tgatgagggc
      661 tttattgtag gtgggccagt tggtgatttt gaacttttgc tttgccacgg aacggtctgc
      721 gttgtcggga agatacgtga tctgatcctt caactcagca aaagttcgat ttattcaaca
      781 aagccgttgc taatcattag tttttgtctt gtggttgaaa actgagcgct atggctatga
      841 cctaggagtg ggagtgaaaa gattcaagga tcctcttgtg atcctttttt ttgcgcgtaa
      901 tctgctgcct gtaaacaaaa aaccaccgct accaacggtg gtttgtttgc cggaatctag
      961 agctaccaac tctttcttcc gaaggtaact ggcttggtgg agcacagata ccaaatactg
     1021 tccttctagt gtagccgcag ttaggccacc acttcaagaa ctcttaatat ctcaatccac
     1081 cttgtccagt taccagtggc tgctgccagt ggcgctttgt cgtgtcttac cgggttggac
     1141 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1201 cagcccagct tggagcgaac gacctacacc gaactgagat accaacagcg tgagctatga
     1261 gaaagcgcca cgcttcccga agggaaaagg cggacaggta tccggtaagc ggcagggtcg
     1321 gaacagagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1381 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1441 cctatggaaa aacgccatcg gcgtggcctt tctcccggcc tttgcctttt gcgcacatgt
     1501 tctttccggc gttatccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     1561 taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga
     1621 gcgtcggaag ctgcatttct ccttacgcat ctgtgcggca tttcacaccc ggcatggcgc
     1681 acttttcata caatctgcac tgatgccgca tggttaagcc agtatacact ccgctatcgc
     1741 tacgtgactg gtcagggctt cgccccgaaa ccccctgacg cgccctgacg ggcttgtctg
     1801 ctcccggcat ccgctcacaa gacaagctgt gaccgtctcc ggagctgtat gtgtcagagg
     1861 ttttcaccgt cacccccgaa gcgtgcgagg cagctgcggt aaagctcgtc ggcgtggtcg
     1921 tgaagtgatt cacaaatatc ggcctgttca tctgcgtcca gttcgttgag cttctccagc
     1981 agcgttaatg tctggcttct tataaagcgg gccatgttaa gggcggtttt ttcctgttta
     2041 gtcactgatg cctccgtgta agggggattt ctgttcatgg ggtaatgata ccgatgaaac
     2101 gcgagaggat gctcacgata cgggttactg atgatgaaca tgcacggtta ctgaacgctg
     2161 tgagggtaaa cagctggcgg tatatggatg cggcggatct gcctggtgag ccggttgccc
     2221 gttccgggaa gctgccgaca ctggcaccgc cgttactgcg ccagctggct gccatcggaa
     2281 taacctgaat cagacggcga ggaaggtgaa cagcgggcag tggtcttccg gtgaccgggt
     2341 tcaggtggtg gccgcactga tggccatcga gagagagttg cgcagcttac gcaggtcgtc
     2401 agggaacatg gagcgcggga tgatagttaa gtttcatgcc aggggaaaag gcgggcagtg
     2461 gtccggttga ttacctgctg ggaagggagc gtaaccggga aggggcaacg gtgcttcagg
     2521 gtgatccgga agaagtccgg gaactcatcg atgccacgcc atttgcgaag aaatacacgt
     2581 caggtgttct gtcgttcgcg gagaaggagc tgccgccggg agggcgtgaa aaagtgatgg
     2641 cgagctttga gcgtgtactg atgcccggtc tcgaaaaaaa tcagtacagc atcctgtggg
     2701 tggagcacca ggacaaggac ggcttgagct gaattttgtc attccgaata tggagctaca
     2761 gaccggaaaa cgctccagcc gtactacgac cgggcagaca ggccgagaat tgatgcctgg
     2821 cagacactgg tgaatcacca ttacgggctg catgacccca acgccccgga gaaccgccgg
     2881 atactgactc tctctgataa cctgcccgga aacgaaacag gcgcttgctg agagcgtcac
     2941 acacgcggga tagacgctct ttatcgtcgg ggagataaaa ggccgtcagg atgtgattca
     3001 ggcgctcacg gaggcaggga ttgaagtggt cagggtgacg cgaagcagta tcagcatttg
     3061 cagacccgaa cggcgggagg aatatcaggc tgaaaggagc attttatgag caatctttta
     3121 cagacgggcg cggagttcga aaaaagctga aagagagagc cgaatctaca gagaaaatgc
     3181 tgaacaacga gttcaggagg ctcggcgaat ctgtaagcga ggctgtgaca tcaaacgaga
     3241 cgaaaatcag agacgctata gccctgttca caacgtcgac agaggaatcg ctgaaaaaac
     3301 accgggaagg ggtgaaagag gcgatgatgc agcaccggaa ggacgtgtta aagctggcag
     3361 ggaatacggg cgtgatgtta ctggggatag tctttctcct gtttaccgcg agtggcggga
     3421 cgctctggta tcttggcggg cggatacagg cgaacctaga agaaatcagg atacaggaag
     3481 agacattgca gaaactgaac gcgaagacat ggggcgtgga gtttgtgcag gacgggaaca
     3541 ggaaatttct ggtcatcccg caggggaaat cggcaacggt gattccctat caggggaagg
     3601 actgggtaca actgacggag tgacacagag tgacggagct ggaaagacat ttgctgaacg
     3661 cattagagca actgcaacag gactatatgc agcggctgaa cgaatgggag agcgccttcg
     3721 tggaattgca gaagatgttt tcgcttacgc aacgggacaa cgcgatgctg aacgagcggg
     3781 tcatgcagtt gagtcagcag gtgcagcact tgagcgagca gacagaaacg cttgagccag
     3841 ttatacagcg agaactggag ataagagagg aacggctgat acaggagcgc gaacatgcat
     3901 tatccctgga acgggagcgt cagccggaaa tacaggaacg cacgctggat ggtccttcgc
     3961 tgggatggtg aaaccatgaa aaatggcagc ttcagtggat taagaggggt taatgtggcc
     4021 tgtaccatct ggttgcatag gtattcatgc ggttaaaatt tatcaggcgc gatcgcggca
     4081 gtttttcggg tggtttgttg ccatttttac ctgtctgctg ccgtgatcgt gctgaacgcg
     4141 ttttagcggt gcgtgtaatg cgacgttatg gtaaatagat gagttaggcc taagtcatga
     4201 cagataaaac gaccgatatt aaaatggctt tagaaaacat gatcagattt cgtgacacta
     4261 tagagagatt gggaggttct atggcgaaag agtttgatgt tgagaccaaa ggaaaacgta
     4321 cttattctat gaataacgat atgagtgatt tatataatgt aatatcacgg gctgttgaag
     4381 cgtctggcgc cgatcattca attaatgaaa aattgacaaa tgtcttgaaa agagaattag
     4441 ttgattatgt caggcattgc gcatctaaaa accaaattgt ctgtattata tgagtttgaa
     4501 aagaattatt tgcagcttat cgcagaatat aaggaagaaa taaaatttgc ttcctctttg
     4561 caagaggatt tacgtaaaga acgtgctaaa ttcttttctg agacattaaa agaggttcat
     4621 caaactctaa atgaatctca agttgataat gaagtggcat caaaatggat taaagaactt
     4681 gacgtatagg aaattgaaaa accagtcgat accattgtcc acgccttatg ccacgtgagc
     4741 ctttctgctt ttcccaccac tccggtggtt tccctctctg tatggtcgat ttttataccg
     4801 taacaggctg ttttttatac tttcaccgat tcccgggcac acttagcctg tgtcaggccg
     4861 gcatatacct cagtagactg atatcccgcg cgtttttctt cagccctgat acattcagcc
     4921 ccgcaatggc ccggcggtat accatccggc agccacagca cgcattcgaa cgggtcacga
     4981 ctcaggaact gtttcaccat ttgtgcatag cacactttcg ccactggttc cggtttatcc
     5041 atccccagtg cacggtacac ctgcggcagc ttctctccac acacacggtt ggcaaggaac
     5101 ccgaagtacc tcaccatctt aaaaaacttc tccgggatgt gctgtttcag cctcgcgacc
     5161 agctcacgct gtgtcagcgt ttccgtcgcc gtttctcccg ttttgtggtc caggtaacgg
     5221 aagctcaggc ttgcccctcc gttgtaatga gccagtcggg aagccgctat tggtggcttc
     5281 tcagataacg acccagggta gcgtgccgta ttccgccctc cgctgtcttc ttcgacatgt
     5341 cacatgccag tattttccgc cggatttcag caccaggctt ctccactgtg attccgtcgt
     5401 gatatgtgac aacgactccg gcattgccat cccctctgac cacgctttca gaagcagctg
     5461 ccgcatattc cacatccacc gtgaacgcat cgcgtctttc aggaagctca gctttttcca
     5521 ctgaccatgc ttattcagac ctccacaggt tacagacaca tgtacatgcg gatgccagtt
     5581 gagacgacgg ccatacgtgt ggatggcgca gaagataccg ggttccagcc cccgttttcg
     5641 ggcggcatac agcagattct ccaccgccag acggcacacg tcattcagca gccagcggtt
     5701 gctttcgaac acccggccac agcgtcgcag tcaggaagac gattcagctg tgttgctgtc
     5761 cacaggtctg tggccttctt tccgcaggac gggcaggcac ggctgccgca tgagttggtc
     5821 aggtacttta cgtgctgaca gtccgggtta tcacacccga actctttaca cccagtatcc
     5881 gtgtgccgca ggccagcatt ttggtgacgg cttcaacctc gatatcgcgc agaccgcccg
     5941 catccaggaa ggacgtccag cactggttgg ctgtgaacag acgtttcaga gggcggggga
     6001 gtaaaccgac aatgaggatg gattaaaccc ggtatcctgt agcagggtaa gggctgctgt
     6061 atcgccacgg cgcttcagga tggaggtcgt caccatcgca ggtggtgggt ccatgggttt
     6121 gagcattttc atccagtccc attctcgttg tgagagcttc tcagcaccaa cagagagcag
     6181 aactgtatcg atacttttgc gggtcaacat aaggggatga gtccttaata aaacaggtcc
     6241 tcagagcata catattctgc gggcgtgcgg caacaacagg acgaaccggg gggttttcaa
     6301 tttgcagccg ccaggctgcc gtggttcatc agagaaaaaa tggaatcaat aaagccctgc
     6361 gcagcgcgca gggtcagcct gaatacgcgt ttaatgacca gcacagtcgt gatggcaagg
     6421 tcagaatagc gctgaggtct gcctcgtgaa gaaggtgttg ctgactcata ccaggcctga
     6481 atagcttcat catccagcca gaaagttatg gagccacggt tgatgagggc tttattgtag
     6541 gtgggccagt tggtgatttt gaacttttgc tttgccacgg aacggtctgc gttgtcggaa
     6601 gatacgtgat ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccccaatt
     6661 aaccaattct gattagaaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     6721 caggattatc aatgccatat ttatgaaaaa gccgtttctg taatgaaggt gaaaactcac
     6781 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     6841 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     6901 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     6961 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     7021 tcattcgtga ttgcgcctga gcgagacgaa atacgtgatc gctgttaaaa ggacaattac
     7081 aaacaggaat cgaatgcaac cggcgcagga acgctgccag cgcatcaaca atattttcac
     7141 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
     7201 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     7261 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     7321 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     7381 ctgattgccc gacattatcg cgagcccata tatacccata taaatcagca tccaggttgg
     7441 aatttaatcg cggtctcgag caagacgttt cccgttgaat atggctcata acaccccttg
     7501 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     7561 caatgtaaca tcagagattt gagacacaat gtggttcccg ccatggcttt gttaatggaa
     7621 tggtcgcctc ccatctcttg tgccacagtg ggacgataaa tcgtcactca ttgaacggag
     7681 cgctgctatc atgaacgtta aaactattgg aatcgatttg gcaaaaaacg ttttccagat
     7741 ccatggggtt gacgagcacg gaaaacggtt gttcaacaaa caactcagac gggcacaaat
     7801 ggcctccttt tttgccaaca tcccaccctg tttgatcggc atggaggcct gtgcatctgc
     7861 tcatttctgg gccaataaac tgatatcgat gggccataat gtcaaactga tggcccctca
     7921 gttcgtcaaa ccctatgtta aaaccaataa gcatgatgct gcagacgctg aagctatttg
     7981 tgaagccgtc actcgaccta acatgcggtt cgtgccggtc aaaaccgctg agcagcaagc
     8041 cgtattggca cttcaccgga gtcgtcagag cttcatcaaa cagcgaaccg cacaagccaa
     8101 tcaaatcagg gggttattgg ccgaatttgg cattgtcgtc ccccgaggta tccagcagct
     8161 acagcgacga ttacctgagc tcgtggaaga tgcggataac ccgttacccg tcctgtttcg
     8221 tacacagctg agtctactac agcaccacat ggcgtacctg ttcgatgtca tcgctacact
     8281 cgacaagcag attgagcagt gctatcggca aaatgctctc tgccagcgta tcggcaagat
     8341 ccctggtatt ggccctgtta ccgccagcgc gctgattgcg accattggta aagccaacaa
     8401 tttcgagaat ggccgacaac tggctgcctg gctcggattg gttccacgtc agcactccag
     8461 tgggggtaaa caagtcctgc tcgggataag caagcgaggt gatacctatt tgaggacctt
     8521 gcttatccat ggtgccaggg cggtattgca gtcggccaaa cataaacagg atgccgtatc
     8581 gagctgggct aaccagctaa tggcgcgccg gaataacaac attgcctcgg tagcattggc
     8641 taacaagaat gcgcggactg tgtgggcgct cctggccaaa gagcgggagt attgtgcacc
     8701 aataataagc gcttaagttg cttaatcagt aagacagaaa caacaccacc gattgcccag
     8761 gcaagcatga agtgatggca agacaggtca gaccgcgatg gggaaaaccc ggtttattca
     8821 aggctcctga aagagcgctt tgttgataga ggcctccatc agcgtattcc atcagggaca
     8881 gaggtatttt acatcacctc ggcaaagtcc ggatctatgg ctgcaatcga tgaccagtaa
     8941 agccaccaca acatatttag cttggcaaac aggaggcgac catctatgaa taaatcagat
     9001 ttcgggtaag tctcccccgt agcgggttgt gttttcaggt aatacgcacg ctttcaggca
     9061 tacctgcttt cgtcattttg ttcagcgctc gtaccagggc catagcctcc gcaacctgac
     9121 catcgtagtc acgcagcgtc agtgaccccc cgaacagctg ttttacccgg tacatcgccg
     9181 tttccgctat cgagcgacgg ttgtaatctg ttgtccattt ccaccgcgca ttactcccgg
     9241 tcattcgctg
//
