LOCUS       NZ_CP097664            12944 bp    DNA     linear   CON 19-JUN-2024
DEFINITION  Klebsiella pneumoniae strain IR1230_1 plasmid unnamed1.
ACCESSION   NZ_CP097664
VERSION     NZ_CP097664.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28095488
            Assembly: GCF_023573705.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12944)
  AUTHORS   Ling,G.
  TITLE     Genomic analysis of KPC-2-producing Klebsiella pneumoniae ST11 from
            Respiratory Departments of a Tertiary Care Hospital in Beijing,
            China, 2010-2018
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12944)
  AUTHORS   Ling,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2022) Microbiology, General Hospital, Fuxing,
            Beijing 100853, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023573705.1-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 11:48:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,160
            CDSs (total)                      :: 6,038
            Genes (coding)                    :: 5,782
            CDSs (with protein)               :: 5,782
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 112 of 256
            Pseudo Genes (incomplete)         :: 147 of 256
            Pseudo Genes (internal stop)      :: 25 of 256
            Pseudo Genes (multiple problems)  :: 27 of 256
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097664.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12944
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="IR1230_1"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed1"
                     /geo_loc_name="China"
                     /collection_date="2022-01-19"
     gene            complement(131..1045)
                     /locus_tag="M3X90_RS26405"
                     /old_locus_tag="M3X90_26405"
     CDS             complement(131..1045)
                     /locus_tag="M3X90_RS26405"
                     /old_locus_tag="M3X90_26405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039356850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="WP_065810610.1"
                     /translation="MEYFSDKERGPVQRINNEVTTKVWAGLVSYINVLVNNGYFGKDFP
                     EECPDGQGCVGTEVKDLKAALQAEIPNLDWPLATEVESEESSSWSREMIPFVPNYLDVM
                     DLLQFCYKHVAIPVKGGYHSYFRHYHFDDFQIEAGKEEFRKKVNTIFARNGLAYELLVS
                     GDIIRMLSPELKQMMASISIPVEKELRSMLMRANEKIINYDVTIRYDALKELWDFWERL
                     KSISYPTDKRESVKKLLDAAAHTSEFRSILEIEAKALTDIGNSYFIRHTEMKQIKIQES
                     AHIEYLYQRMFSLIHLLLKTLPS"
                     /name="AbiJ-NTD4 domain-containing protein"
     gene            complement(1115..2283)
                     /locus_tag="M3X90_RS26410"
                     /old_locus_tag="M3X90_26410"
     CDS             complement(join(1115..2034,2034..2283))
                     /locus_tag="M3X90_RS26410"
                     /old_locus_tag="M3X90_26410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc52 family transposase"
                     /protein_id="WP_114473046.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADAASAMDVGLSTMTRWVKQ
                     LRDERQGKTPKASPITPEQIEIRELRKNLQRIEMESEIFKKGYRALDVRLPEQFSIIGK
                     LRARYPVATLCHVFGVHRSSYKYWKNRPEKPDGRRAVLRSQILELHGISHGSAGARSIA
                     TMATQRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNHLERQFAVTEPNQ
                     VWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPVG
                     VMFHSDQGSHYTSRQFRQLLWRYRFRQSMSRRGNCWDNSPMERFFRSLKNEWVPATGYV
                     SFSDAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNAVASFC"
                     /name="IS3-like element ISEc52 family transposase"
     gene            complement(2884..3687)
                     /locus_tag="M3X90_RS26415"
                     /old_locus_tag="M3X90_26415"
     CDS             complement(2884..3687)
                     /locus_tag="M3X90_RS26415"
                     /old_locus_tag="M3X90_26415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_250203207.1"
                     /translation="MNTGDNSKLVDNPSQPFDITKLIKQIVQECCYDNGDNSWGNAATQ
                     DLSYCSSLLTRIQAVTYASSLKSVFNSGEEIETLGTILESFLLSNKRVLRLCLSDVSYE
                     FYAREILANVIGRKLLTLARLEKFRLNPLLVIVDEAHNFLGKRVGSEDHAVKLDAFEMI
                     AKEGRKYGLNICLTTQRPRDITEGVLSQIGTLLVHRLTNDRDRDVVERACGEIDKSAAS
                     FLPSLKQGEVALVGVDFPIPMTIQIDKPIYPPESDSPGYSKIWKK"
                     /name="ATP-binding protein"
     gene            complement(3717..4214)
                     /locus_tag="M3X90_RS26420"
                     /old_locus_tag="M3X90_26420"
     CDS             complement(3717..4214)
                     /locus_tag="M3X90_RS26420"
                     /old_locus_tag="M3X90_26420"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="WP_250203208.1"
                     /translation="MDFISLIPELINKGLVSDEEERVGIVLGYISGGSTSIVTVEPDKL
                     FGRHCAILGATGGGKSWTTAKLIEECSNYENSKTIIIDATSEYRSFQSEHCLHYHLSSP
                     INKNINSLEFRIPPTDFIESDFVAMFDPSGKVQGPKLKEAIKSLRLVSLDPTIATDGIL
                     KK"
                     /name="DUF87 domain-containing protein"
     gene            complement(4430..4603)
                     /locus_tag="M3X90_RS26425"
                     /old_locus_tag="M3X90_26425"
     CDS             complement(4430..4603)
                     /locus_tag="M3X90_RS26425"
                     /old_locus_tag="M3X90_26425"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_250203209.1"
                     /translation="MKNITLQLGILRNDLKVGVISSIFAQTVRVNLNYAGEISGTYLDG
                     SRYGKGEVGKYY"
                     /name="hypothetical protein"
     gene            complement(4606..5892)
                     /locus_tag="M3X90_RS26430"
                     /old_locus_tag="M3X90_26430"
     CDS             complement(4606..5892)
                     /locus_tag="M3X90_RS26430"
                     /old_locus_tag="M3X90_26430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="WP_077268095.1"
                     /translation="MATNPDGYLSYLKPGSTDWKALDYKSYIYWRDRRQRKILEDAGTP
                     IPQPLPLSEGENKKLKDNQAYVNEFLLKSLQFPNLSFFAGSGTSLGDVGGPSMWDLWKF
                     AMWENPLLLENGQLKPDAITVCDKVRYSERDYPNIEHFLSNCEAYLAFHDDENVLNFLN
                     QVKLIILDKCRSFIDDGNCDLTAYVNLLQKMARRRVRDPRLKVFTTNYDLTFETAASEL
                     GMMVVDGLSYTGLRRFDGKYFNYDVVKRDENEHEFIEGVFQLFKLHGSVSWIRKDGQIY
                     ESNKPEATNACLIYPAKGKYQQAFIQPHLELLSRLLDFLRKKNNCLVISGFGFNDDHLS
                     EPIYSAIKSNPSMRLIVVDYKCATHIQNNGENGSSKYWPLLMELALSGYDIHFINSSFI
                     DFVKLVPNLKALTPAEQLAKIIKQVGAND"
                     /name="SIR2 family protein"
     gene            6039..7007
                     /locus_tag="M3X90_RS26435"
                     /old_locus_tag="M3X90_26435"
     CDS             6039..7007
                     /locus_tag="M3X90_RS26435"
                     /old_locus_tag="M3X90_26435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224844.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /protein_id="WP_250203210.1"
                     /translation="MKDQITHLHDNADRSVAKQKFKITNWPTYNKALINRGSITFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVPLR
                     CPDYSCVSRQAKSVNVSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRK
                     LHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRAASADGAYDTRLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQQMTGSNARWKWTTDYNRRSIAETAMYRVK
                     QLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
                     /name="IS5-like element IS903B family transposase"
     gene            complement(7680..7937)
                     /locus_tag="M3X90_RS26440"
                     /old_locus_tag="M3X90_26440"
     CDS             complement(7680..7937)
                     /locus_tag="M3X90_RS26440"
                     /old_locus_tag="M3X90_26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197688.1"
                     /translation="MDKKYDFSLSYEALTRVCENAICEHIRRAGSLEGLGFALEYTKAY
                     AILEVWSLLAAAGDTFPALIEKDRIYLLQLISGKNNIELH"
                     /name="hypothetical protein"
     gene            8577..>9092
                     /locus_tag="M3X90_RS26445"
                     /old_locus_tag="M3X90_26445"
                     /pseudo=""
     CDS             8577..>9092
                     /locus_tag="M3X90_RS26445"
                     /old_locus_tag="M3X90_26445"
                     /inference="COORDINATES: protein motif:HMM:NF024202.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /name="CSS-motif domain-containing protein"
     gene            9156..9992
                     /locus_tag="M3X90_RS26450"
                     /old_locus_tag="M3X90_26450"
     CDS             9156..9992
                     /locus_tag="M3X90_RS26450"
                     /old_locus_tag="M3X90_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="WP_004178087.1"
                     /translation="MLLVTVSLLSGLMIYIIIMNRAELNSSLSARFVKALKNEALSLVY
                     QPIYRIEDGQICGFEALLRWKDERLGNISPEVFIPLSEREGLQEDVTLFVINHAIREFI
                     HTAIQNEIFLSVNINPSDLDSEKFRDKLLGLISEYNIPYKTILLEITERQGGDFEGMKI
                     HIDKYKNHGVRFAIDDFGTGYSNLNLVTALDVDEIKIDKSLTSAIGTESLRYDLLPGLH
                     EMFRSIADKIVFEGVETQEQVNYLKTFWPQSYAQGWYYSRALPLEEARKLTIRELN"
                     /name="EAL domain-containing protein"
     gene            complement(10974..12251)
                     /locus_tag="M3X90_RS26455"
                     /old_locus_tag="M3X90_26455"
     CDS             complement(10974..12251)
                     /locus_tag="M3X90_RS26455"
                     /old_locus_tag="M3X90_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="WP_004152070.1"
                     /translation="MISRWKWILKQTVKKLWFRATLFAIVAIITALLSILFKSMIPESV
                     SVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQNV
                     LATFIGSFLFSLVGIIALSMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSLGRV
                     GETTAKVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYINMVKLS
                     KLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSISADLLETIIVSDVRSFAQDPRF
                     CLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVHVAPLQN
                     NDLLEDFFSPVARDGAGMREIQIRVLKGLSMLSKGWPGIFSEAAHNLAFETLEHAIRAD
                     HIDSDRCLIKSIYYNLFSGEDSNKKP"
                     /name="DUF2254 domain-containing protein"
     gene            complement(12314..>12944)
                     /locus_tag="M3X90_RS26460"
                     /old_locus_tag="M3X90_26460"
     CDS             complement(12314..>12944)
                     /locus_tag="M3X90_RS26460"
                     /old_locus_tag="M3X90_26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="WP_250203211.1"
                     /translation="SLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKAL
                     RRDLTKRESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQE
                     ELNKQGTISHISDAVEDRIRLEVDLGNELNFIYEVRLRGYSSPTFALAAMDNNEQQTEQ
                     HRYYRAEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
                     /name="BCCT family transporter"
ORIGIN
        1 ccttcagtag ctgagcagga gggacaactg atggagatgt aagccacggg agcacctcaa
       61 aaacaccatc gtacactaaa tcagtaagtt ggcaccatta cctccatagc ctgctgaaaa
      121 agcaataata tcaacttgga agtgttttta gtaaaagatg tatcaagctg aacatgcgct
      181 gatatagata ttcgatatga gcactttcct gaatcttgat ctgtttcatt tcggtatggc
      241 gaatgaaata actatttcca atatctgtaa gtgcttttgc ctctatttct aaaatcgatc
      301 taaattccga tgtatgggct gccgcatcaa gtaacttctt cacagactct cttttgtcag
      361 ttggataaga tatgcttttg agccgctccc aaaagtccca aagctcttta agtgcatcat
      421 aacgaatggt gacatcatag ttgattattt tttcattcgc gcgcatgagc atgctgcgta
      481 actctttctc tacaggaatg cttatgcttg ccatcatttg cttcagttcc gggcttagca
      541 ttcgaatgat atcacctgat acaagaagct cgtaagcaag gccgtttctg gcaaatattg
      601 tgtttacctt tttcctaaat tcttctttac cagcttcaat ttgaaaatcg tcaaagtggt
      661 aatgcctgaa atagctgtga tagccccctt taacaggtat ggccacatgt ttatagcaga
      721 actggagtaa atccattaca tccagatagt ttggaacaaa aggtatcatc tccctagacc
      781 aagatgacga ttcctctgat tcaacttctg ttgccaatgg ccagtcaaga tttggtatct
      841 cagcctgtaa cgcggctttg aggtctttga cctcggtgcc tacgcatccc tgcccatcgg
      901 gacattcttc aggaaaatct ttaccgaagt atccgttgtt taccagaaca ttaatgtatg
      961 aaacaagccc agcccagact ttagttgtta cttcgttatt tatccgttga acggggcctc
     1021 tttctttgtc gctgaagtat tccacatctg ctcctgtagg tttaatgacc tcacaaattt
     1081 atatctgaat gtgctgtcgg gcaagctgaa gtggtcaaca aaaactggcc accgcgttag
     1141 agtttttcca gtatcggttt tctgattcgt ttggtggtaa cccaccatta tattcgtgcg
     1201 gtcttagtgc gctgtaatat ccaacgatat agtcagttat tgcgtgggct gcatcgctga
     1261 agcttacata gcccgtcgcc ggcacccatt cgttcttcag actcctgaag aagcgctcca
     1321 ttgggctgtt atcccagcag tttccacgcc gactcatact ctgcctgaac cggtatcgcc
     1381 acagtaactg ccggaactgc ctgctcgtat aatgactgcc ttgatcgctg tggaacatca
     1441 ccccgactgg cttaccacgg gtttcccatg ccatttccag tgctttcatg gtgagcctgc
     1501 tgtccggcga gaacgacatg gcccagccca ctggttttct tgcgaacagg tcgagaacaa
     1561 cggcgaggta cgcccagcgc ttacccgtcc agatataggt cacatcaccg caccacacct
     1621 gatttggttc cgttacggcg aactgtcgct caagatgatt cgggatagca acgtgctcat
     1681 gaccaccacg cttataccgg tgagtcggct gctggcaact gaccagcccc agctctttca
     1741 tgagtctgcc agcaagccag cgccccatct ggtatcctct ctgggttgcc attgtggcga
     1801 tgcttcttgc tccggcagag ccgtggctga tgccatgcag ttcaagtatc tgactgcgta
     1861 atacagcccg tctgccgtct ggcttttcag gacggttttt ccagtatttg tagctgctgc
     1921 gatgaacccc gaacacatgg cagagagtgg ccacaggata acgcgccctg agtttcccga
     1981 ttatcgagaa ctgttcaggg agtctgacat caagagcgcg gtagcctttt ttaatatttc
     2041 actttccatt tcaatacgtt gtagattttt tctgagctct cggatttcaa tttgttccgg
     2101 ggtaatgggt gaggcttttg gtgtttttcc ctgccgctca tcacgtaatt gtttcaccca
     2161 tcgcgtcatt gtggaaaggc cgacatccat agcgctggct gcatctgcca cggtatagtt
     2221 ctggtcaacg accagttgag cggattcgcg tttaaactct gcgctgaaat ttcttttttt
     2281 cattatggca cctgtgttgt tctgaggtga gcatatcacc tctgttcagg tggccaaatt
     2341 cagtaaacca cttcaccagc gccgcttatt cccatgccgt ggtattagcc ttccgcgatg
     2401 ggttttgcca gcggtctaac aaccgcaata agtcccggct ctcctccggg ccattttgcc
     2461 atgatgttaa ctggcacgag cgcggcgcgt ttggggtttc tccccccatc aatgggcgtt
     2521 gccgccgctc ccttattcca atctctcaga aaaataacga taaagatcct cgttgttcct
     2581 caggggcggt atctccgccg cccgcctttt ttctgatgca gatacaaata aaggaaggtc
     2641 gccactgggc gacctctttt gtactcgcac gccgtcaatt ctgacgatac ggcccgaaag
     2701 gccaaagcct acaggccagc aaaccacttc aacgtccgag catttggaag agcggagact
     2761 gatggtgcag tggtgggctg attaccttta tataaatgca cagggctata taacgcccta
     2821 tgattttggt aaaagcccta tttaattgat gttacttcaa acagggtcgt aagaccctgt
     2881 atatcatttt ttccatattt tactgtatcc tggagaatca gattcaggag gatatatagg
     2941 cttgtctatt tgtatagtca tggggattgg gaaatcaaca ccaaccagag caacttctcc
     3001 ttgcttgagg cttggtaaaa atgatgctgc agatttatca atttcaccac aagctcgctc
     3061 aacaacatct ctatctctat cattcgttaa acgatggact aacaaggttc caatttgact
     3121 aagtacacct tctgtaatat ctctaggcct ttgtgttgtt aggcaaatat ttaggccata
     3181 ctttcgacct tcttttgcga tcatctcgaa agcatcaagt ttaaccgcat gatcttctga
     3241 tcccactcgc ttaccaagaa agttatgggc ttcgtctact atgactaata atggattaag
     3301 tctgaatttt tctagacgag ctagtgttaa aagctttctt ccaatcacat ttgcaagaat
     3361 ttctcttgca tagaattcat agcttacatc actaaggcat aacctgagca cccgtttatt
     3421 agacaataaa aaggattcta atattgttcc taatgtttca atttcctcgc cactattaaa
     3481 aacagatttt aatgagctcg cataagtgac agcttgaatt cttgttaaaa gagaagagca
     3541 atagcttaag tcttgagttg cagcgtttcc ccaagaattg tcaccattgt cgtaacaaca
     3601 ttcctgaacg atttgcttta tgagtttagt tatatcaaaa ggttgggatg gattatcaac
     3661 aagttttgag ttatctcctg tattcatcgc gcggcgataa tctgttttag gttggtttat
     3721 tttttcaaaa taccatcagt tgcaatggtt gggtctaatg atactaatct aaggcttttt
     3781 atggcctctt taagtttagg tccctgaacc ttaccagagg gatcaaacat agcaacaaaa
     3841 tctgattcaa tgaaatcagt aggtggtatt ctaaactcta aggagttaat gtttttattt
     3901 attggtgagc ttaaatggta atgtaagcag tgttctgatt gaaatgatct atattcgctt
     3961 gttgcatcaa tgattattgt cttggaattt tcataattag agcattcttc tatcagttta
     4021 gctgttgtcc agctttttcc tcctccagta gcaccaagaa tggcgcaatg tcttccaaaa
     4081 agtttatctg gctcaactgt gactatactt gtagaacctc ctgaaatata tcctaaaaca
     4141 attccgaccc gttcttcctc atcacttacg agacctttat taattagttc tgggattaaa
     4201 gatataaagt ccaaaggagc agagtaaact ctatcaccta atcgagggta ggattttatt
     4261 cctgaatcaa tacggaaatt cgatggatct attgtaccaa gtaattgaat tactcctacg
     4321 gcatcaactt tatagttctt atgtaactca ccagatatct ctcctctatc acgttcgggt
     4381 agtttaactt ctattaatcg ccctaatact atggattgtt gaccttcaat taataatatt
     4441 tcccaacttc gcctttacca tatctagagc catctaaata tgtgccgcta atttcaccgg
     4501 cataatttag gttaactctg acggtttgag cgaaaattga tgaaataact cccaccttta
     4561 gatcatttct tagaattcca agttgaagtg ttatgttttt catatttaat cgttagctcc
     4621 aacttgtttt atgattttag ctaattgttc ggctggtgtt aatgctttga gattaggaac
     4681 tagtttgaca aaatcaataa atgaggagtt tataaaatgt atatcatatc ctgataatgc
     4741 aagttccatt agcaatggcc aatatttgct tgagccgttt tctccattgt tttgtatatg
     4801 ggttgcacat ttataatcca caacaattaa tcgcatgctc ggattcgact taattgctga
     4861 ataaattggc tcagaaagat gatcatcatt aaaaccaaat ccagatatca ctagacaatt
     4921 attctttttt cttaagaaat caagaagtct tgaaagtaac tccaagtgag gttggataaa
     4981 agcttgttgg tatttacctt tggcaggata aattagacaa gcgtttgtgg cttcaggttt
     5041 gttagattca tagatttgcc catcttttct tatccaacta actgagccgt gaagtttgaa
     5101 taattggaat acaccttcta taaattcgtg ctcgttttca tctcttttca cgacatcata
     5161 gttaaagtac ttaccatcga acctacgaag gcctgtatag gaaagaccat ctactaccat
     5221 cattcctaat tctgaagcag ctgtttcaaa agttaaatca tagttagtgg taaaaacttt
     5281 aagcctaggg tctcttactc ttcttcttgc cattttttga agtaaattca cgtaggctgt
     5341 aagatcgcaa tttccatcat caataaatga acgacatttg tctaaaataa tcaatttaac
     5401 ttggtttaaa aaattcaata cattttcatc atcatggaaa gctaagtatg cttcacaatt
     5461 tgaaagaaag tgttcaatgt ttgggtagtc tcgttctgaa tatcttactt tatcacaaac
     5521 agttattgca tctggcttca attgcccatt ttctagcaat aaagggtttt cccacattgc
     5581 aaacttccaa agatcccaca tgcttggtcc accaacatca ccaagtgacg tgccgctacc
     5641 agcgaagaat gagaggttag ggaattgtaa tgattttaat aaaaattcat taacatatgc
     5701 ttggttgtct tttaattttt tattttctcc ttcggataga ggtaatggtt gcggaattgg
     5761 tgtgcctgca tcttctaata tttttctctg cctgcgatca cgccaatata tatatgattt
     5821 ataatctaat gctttccagt cagtactgcc tggtttcaaa taagagaggt atccgtcagg
     5881 attggtagcc atgatcatta tccattgatg tcgtcaggaa gttgtccctt caatttacta
     5941 cacatgatga agaagaaaag taaccataaa tacaatatta aaatatcttg cgttcaatac
     6001 gttactgcct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc
     6061 ttcacgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggccca
     6121 cctacaataa agccctcatc aaccgtggct ccataacttt ctggctggat gatgaagcta
     6181 ttcaggcctg gtatgagtca gcaacacctt cttcacgagg cagacctcag cgctattctg
     6241 accttgccat cacgactgtg ctggtcatta aacgcgtatt caggctgacc ctgcgcgctg
     6301 cgcagggctt tattgattcc attttttctc tgatgaacgt tccgctacgc tgcccggatt
     6361 acagctgtgt cagcaggcag gcaaagtcgg ttaatgtcag tttcaaaacg cccacccggg
     6421 gtgaaatcgc acacctggta attgattcca ccgggctgaa ggtcttcggt gaaggcgagt
     6481 ggaaagtcaa aaagcatggc caggaacgcc gccgtatctg gcgtaagctg catctcgccg
     6541 ttgacagtaa aacacatgaa atcatctgcg ctgacctgtc gctgaacaat gtgacggact
     6601 cagaagcctt cccgggtctt atccggcaga ctcacagaaa aatcagggca gcatcggcag
     6661 acggcgctta cgacacccgg ctctgtcacg atgaactgcg gcgtaagaaa atcagcgcgc
     6721 ttatcccacc ccgaaaaggt gcgggttact ggcccggtga atatgcagac cgtaaccgtg
     6781 cagtggctaa tcagcaaatg accgggagta atgcgcggtg gaaatggaca acagattaca
     6841 accgtcgctc gatagcggaa acggcgatgt accgggtaaa acagctgttc gggggttcac
     6901 tgacgctgcg tgactacgat ggtcaggttg cggaggctat ggccctggta cgagcgctga
     6961 acaaaatgac gaaagcaggt atgcctgaaa gcgtgcgtat tgcctgaaaa cacaacccgc
     7021 tacgggggag acttacccga aatctgattt attcaacaaa gcctataaaa gaaataaaga
     7081 aaaagatgaa actggctagc acatatgagt tgtatcaatt cgcgtatatg ttaaaatcac
     7141 tcacggactc gacctaatct ccttttttaa ttgttaacac aggtattttt atgttattgt
     7201 atgttgtttt gtctatttcg ctggttttat tatatgggat gataataaaa tataaagtga
     7261 agtcaaggaa atcacagggt actgtaaaaa agcctatggc aatcagaaat aatagaaatc
     7321 taaagtagat tcaaagagaa tagctttcgg gtataaatgt agttattccg tcttcagtaa
     7381 gttaatgata aatgctttac tctacctgat gttatacagg acgagttttt ctcactttta
     7441 tctaatgtgt atattgtttt ttctttatat ctaaaactga agtcattgat tccagacagg
     7501 gcgcgatgac ggtagttatc ccgccttgtc atcgcacaag aatactgaaa ctctacggcc
     7561 aatccgactg accagaataa tcagaattat tattgcacaa caaatttcat tttcctcacg
     7621 tcagtgattc cggcgggcgt taagtaaaaa cggagatgtt ctgccaaata atttatgatt
     7681 taatgtagct ctatattatt tttcccggaa attagttgca acagatatat tctgtctttc
     7741 tcaatgagag ctggaaatgt atcgccagca gctgccagca gagaccatac ttccaggata
     7801 gcataggcct ttgtatattc aagtgcgaaa cctaaccctt ccagagaacc tgctctgcga
     7861 atatgttcac atattgcatt ttcacatacc cgtgttaagg cttcataact gagcgaaaaa
     7921 tcatattttt tatccatctt aagctatctt cacccaaatg cagatatccg gttacacgtt
     7981 gcaatgcgta acattatcaa taataacccg attataacat gaattttcgt tttgtaatgc
     8041 cgatgtcaca caacatttta ctctgttttt ttcaaaaaag agtgtcttta ttttggtgta
     8101 caccttagtg tgcgatcgcc cagcaagaaa tgaatattcg ttctatacta gtgggtaaaa
     8161 aaccgcatta catgcatcgc ttaaaagttg caccatgcat ctggagcctt cacgaggcta
     8221 ttaataacgc atgaattatc tctgtaaatc ataaccagta tctatccgaa ccatgtcaca
     8281 ttatttgatt catacccggg atgagtaagg caagaatagg tatactggaa tagtatgttc
     8341 agtgccagct aatcggtcag tgaagttggc gaaaatatag ctttatatct tttcaatttg
     8401 aggggagatc tgcatggtta gtcgtaagct ggaacgtttg attagccctg gaaagtggat
     8461 acataatcta tcagtcctct ctttatcgat tctttcagct ggtatatttt tttgtattat
     8521 ctgagagcgt atcttacctg atttttcggg ataaaattaa agactatact tccgatgtgc
     8581 ttgttcgctc tgaatcgctc ctggttcaga ctgaatctgt caagactgct gcaaagtcac
     8641 atttgaatat aggagacagt ccatgtacta atgaaaatat cctacatctt cgggttgtag
     8701 tatggcccta tccgttgatt aaagatgttg gctacatcat aaaaggagaa ttagcctgta
     8761 gtgctctgtg ggggagttta cgtcccacct taagtctgga acattttgat aaaaaatgga
     8821 cttcgcggga aggagtgtgg ctttttggta ttaagcttat ggatgatata tccgtcaatg
     8881 gatatatttc tgaaggtatt atggtgaccc tatcaccatt tgtcttcaga cgattcgaaa
     8941 ctgatatgta ttcaaaaaac tttagcgctg ttgtcggaaa tagtaagcat gacaggcatt
     9001 acttcaatat aggacctgat gctcctcttc ttgatcggca tgatagcgtt ccactaaggt
     9061 ttagagtgtt tagggcatgt agcctacatt atgatctctg tgttgcagga ggtgggtatt
     9121 ttacggggat attttcagag cactggagca tacaaatgtt attagtaaca gtgtcactac
     9181 tttccggttt aatgatttat attatcatta tgaacagagc agaactcaat agttcactat
     9241 ctgcaaggtt tgtaaaggct ttgaaaaatg aagccctgtc gctggtctat caacctattt
     9301 acagaattga agacggacag atctgcggat ttgaggcctt gctcagatgg aaagatgaaa
     9361 gactcgggaa tattagccct gaagtattta taccgctttc tgaaagggaa gggttgcagg
     9421 aggatgtgac tttattcgtc attaaccatg ctatccgaga atttatccat actgctattc
     9481 agaatgaaat atttttgagc gtaaacatta atccaagcga tcttgattca gaaaaattca
     9541 gggataaact actaggatta atttctgaat ataacatccc atataaaaca atccttcttg
     9601 aaattacgga gcgtcaagga ggggattttg aaggtatgaa aattcacatt gataaatata
     9661 aaaatcatgg tgtcaggttt gccatcgatg actttgggac aggatattct aatcttaatc
     9721 tggtaactgc gcttgatgta gatgaaataa aaatagacaa atcgctaact agtgctattg
     9781 gtactgaatc tttacgatat gatcttcttc ccggactgca tgagatgttc cgctccattg
     9841 cagataagat tgtgtttgag ggtgtagaga ctcaggagca ggtgaactat ctgaagacgt
     9901 tctggccaca gagttatgct cagggctggt attactccag agcgcttccg ctggaagaag
     9961 cgagaaaact taccatccgg gaattgaact gaccggcact ttcagcgcaa acactcagca
    10021 attgcttgcc gatcagctgg tagaaattga agggcagtgt aatcacctgc tgctggcgca
    10081 ggggaaagcg ttactgaaaa gtcgttctga actgcttaaa taacagagaa accaggatgt
    10141 gatttgatgc aaaacacgga acagcgaatg taatatttac tgagatgagc gagctttgtc
    10201 tcatagtaca gaggagtcgg ggctgccagt atccggtaga cagcattatt cgtctgcaac
    10261 ataactctgc tggttgctgc ggataactac tcagcgcctt ccagagatgg cattgatgaa
    10321 agtagtagaa aaaacctgga atctttgaag atgtgtccct acctcagggg ctggagtgcc
    10381 atctggccct aattcagcac agtgctgcag atatgtgtta tgtacaagta gacactgtct
    10441 tgaaaatcag aagtcgttga gggttgaatg caattccaat atttgcttca gcagataacg
    10501 aggaccgcta tctattgcat ctagtgaata ggaagccgtt tccatttttc tctaaagcaa
    10561 ctggtgttga catcctgaat tccttcaaat gtctgatatt ccttttttca aagcactaga
    10621 aaaatacccg aaagctgtta atctaataca ttgactgacc atacagtaca gcgttatttt
    10681 gttgggttta cttcacgtac tttttattgt cggatactgt tgggaaaaga cacaaagaag
    10741 caagccatta agcggaatac tgcgtagggt tagaatatac aattaacttt tccgtgatag
    10801 gactagtgat aacaatattt ccagcatggg ctgacaggag tatagatgga actgctctcc
    10861 ataggttaat acatctcgca attaacacgc ccgcatatgc cacaaaactg acgcccattc
    10921 tcggaatttt ttttgccatt atctttcctg cggcctaatt actttcccca caactatggt
    10981 tttttattag agtcttcacc actaaataaa ttataataaa ttgattttat tagacatctg
    11041 tcagaatcta tatggtcagc acgtattgca tgctctaatg tttcaaatgc taaattatgc
    11101 gcggcctcag aaaatatccc aggccaaccc ttactcagca tagacagtcc tttaaggact
    11161 cttatctgta tctccctcat accggcacca tcgcgcgcga caggtgagaa aaagtcttcc
    11221 agtagatcgt tattctgaag tggtgcaaca tgaactgaag gatatttcac ttctatttca
    11281 tcagatttat tctgcgcgta agtggaaagt atacgaacac ctctgccaat gacatcaatg
    11341 gcggttccag gatcgttcac tgcgggggaa agtgcccggc aggctatttc ggccatgacg
    11401 ctaagacaaa atcggggatc ctgagcaaat gaacgtacat ccgagacaat aatcgtctca
    11461 agtaaatcgg cgctgattga tgactcctgg ccttgactca ggtacaaaac gggcatggat
    11521 ggatggatga aactgcctgg ctgcgccacg aggtatacat gacggggatc attggtcagc
    11581 agcttgctaa gtttcaccat attaatatat tcaacatagc caatcttctt cggataaact
    11641 gcaaccgttc ctttcggctg ttcattgttc tcaagccatg gatatccgcc gagacaggga
    11701 tttcttgctc tcgcaataaa tgtttcgatg gccgcctgtt ctacttttgc cgttgtctca
    11761 cctacccttc ccagagaggt caaatgctgt atccagcgaa gcaatgtgat gaggattaag
    11821 gcaatgacaa ccagtgtgac aatgaataaa atgactctcc ccctttctcc ataagctccc
    11881 atactgaggg caataatccc taccagactg aagagaaaag aaccgatgaa ggtggccagt
    11941 acattttgtg tggttacgtc ttcaacaact aaacgcgtag ctctgggagt cacattagta
    12001 gtggctgaac cgtaggctgt gaccatgata ctcaacgaaa aagtggtcac tgccagcata
    12061 ctcgatgcca gtatgttcag aatgttatcg actgcttccg caccaacctt cacggaaacc
    12121 gactcaggta tcattgattt aaaaagaatt gataaaaggg ccgttattat cgcgacaatg
    12181 gcgaataacg ttgccctgaa ccatagcttt ttaactgtct gcttcagaat ccatttccag
    12241 cgtgaaatca ttctgcattc ctcatattgc ggccgggata aataattgcc gcccccagga
    12301 cggcatgttg ctattaacga acgaggtgca ggaagtgcag atgtttttcg tactggtcca
    12361 gtatgtcatt aatcagctgc tcctggttcc agcccatcac atcataattt tgaccgcctt
    12421 ctttgagata tacctcagcg cgataatatc tatgttgttc agtctgctgc tcattattat
    12481 ccatcgcggc gagcgcgaag gtcggtgagc tatacccgcg aagcctcact tcatatataa
    12541 aattcagctc gttacccaaa tcgacttcaa gacgaatacg atcctcgact gcgtcactga
    12601 tgtggcttat cgtcccctgc ttgttcagct cttcctgaac cagcgtcatg gcgggctgga
    12661 tgacgtcgtc cataaaacgt ttcacaagag atcgcttcgg cagatacgcg atattgcgta
    12721 accttctctg ccagggaatt gggttacgtg cagccgtagg agcaattgtc gccatgctca
    12781 ggctttcacg cttggtcaaa tcccggcgca aagcttttaa aagtccgtat atggatatca
    12841 gtaagatcac tgagaaaggc aatgcactcg ctattgtcac tgtttgcagc gcacttagcc
    12901 ctccggcaag gagaagcgca attgcgacaa tgcccatgag cgag
//
