LOCUS       NZ_CP097661            12798 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Klebsiella pneumoniae strain IR12243_1 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP097661
VERSION     NZ_CP097661.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28095489
            Assembly: GCF_023573685.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12798)
  AUTHORS   Ling,G.
  TITLE     Genomic analysis of KPC-2-producing Klebsiella pneumoniae ST11 from
            Respiratory Departments of a Tertiary Care Hospital in Beijing,
            China, 2010-2018
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12798)
  AUTHORS   Ling,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2022) Microbiology, General Hospital, Fuxing,
            Beijing 100853, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023573685.1-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 11:20:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,675
            CDSs (total)                      :: 5,553
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 220
            CDSs (without protein)            :: 220
            Pseudo Genes (ambiguous residues) :: 0 of 220
            Pseudo Genes (frameshifted)       :: 133 of 220
            Pseudo Genes (incomplete)         :: 92 of 220
            Pseudo Genes (internal stop)      :: 17 of 220
            Pseudo Genes (multiple problems)  :: 22 of 220
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097661.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12798
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="IR12243_1"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed1"
                     /geo_loc_name="China"
                     /collection_date="2022-01-19"
     gene            join(12711..12798,1..227)
                     /locus_tag="M3X86_RS27105"
                     /old_locus_tag="M3X86_27105"
     CDS             join(12711..12798,1..227)
                     /locus_tag="M3X86_RS27105"
                     /old_locus_tag="M3X86_27105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            391..819
                     /locus_tag="M3X86_RS27110"
                     /old_locus_tag="M3X86_27110"
     CDS             391..819
                     /locus_tag="M3X86_RS27110"
                     /old_locus_tag="M3X86_27110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            845..1024
                     /locus_tag="M3X86_RS27115"
                     /old_locus_tag="M3X86_27115"
     CDS             845..1024
                     /locus_tag="M3X86_RS27115"
                     /old_locus_tag="M3X86_27115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1051..1581)
                     /locus_tag="M3X86_RS27120"
                     /old_locus_tag="M3X86_27120"
     CDS             complement(1051..1581)
                     /locus_tag="M3X86_RS27120"
                     /old_locus_tag="M3X86_27120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1588..2319)
                     /gene="mobC"
                     /locus_tag="M3X86_RS27125"
                     /old_locus_tag="M3X86_27125"
     CDS             complement(1588..2319)
                     /gene="mobC"
                     /locus_tag="M3X86_RS27125"
                     /old_locus_tag="M3X86_27125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(2319..4283)
                     /locus_tag="M3X86_RS27130"
                     /old_locus_tag="M3X86_27130"
     CDS             complement(2319..4283)
                     /locus_tag="M3X86_RS27130"
                     /old_locus_tag="M3X86_27130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5767..5916)
                     /locus_tag="M3X86_RS27135"
                     /old_locus_tag="M3X86_27135"
     CDS             complement(5767..5916)
                     /locus_tag="M3X86_RS27135"
                     /old_locus_tag="M3X86_27135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6001..6258)
                     /locus_tag="M3X86_RS27140"
                     /old_locus_tag="M3X86_27140"
     CDS             complement(6001..6258)
                     /locus_tag="M3X86_RS27140"
                     /old_locus_tag="M3X86_27140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(6268..7953)
                     /locus_tag="M3X86_RS27145"
                     /old_locus_tag="M3X86_27145"
     CDS             complement(6268..7953)
                     /locus_tag="M3X86_RS27145"
                     /old_locus_tag="M3X86_27145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(8058..>9194)
                     /gene="ltrA"
                     /locus_tag="M3X86_RS27150"
                     /old_locus_tag="M3X86_27150"
                     /pseudo=""
     CDS             complement(8058..>9194)
                     /gene="ltrA"
                     /locus_tag="M3X86_RS27150"
                     /old_locus_tag="M3X86_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220828.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /name="ltrA"
     gene            9259..9963
                     /locus_tag="M3X86_RS27155"
                     /old_locus_tag="M3X86_27155"
     CDS             9259..9963
                     /locus_tag="M3X86_RS27155"
                     /old_locus_tag="M3X86_27155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(<10017..10373)
                     /locus_tag="M3X86_RS27160"
                     /old_locus_tag="M3X86_27160"
                     /pseudo=""
     CDS             complement(<10017..10373)
                     /locus_tag="M3X86_RS27160"
                     /old_locus_tag="M3X86_27160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /name="group II intron reverse transcriptase/maturase"
     gene            11018..11263
                     /locus_tag="M3X86_RS27165"
                     /old_locus_tag="M3X86_27165"
     CDS             11018..11263
                     /locus_tag="M3X86_RS27165"
                     /old_locus_tag="M3X86_27165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            11537..11908
                     /gene="tolA"
                     /locus_tag="M3X86_RS27170"
                     /old_locus_tag="M3X86_27170"
     CDS             11537..11908
                     /gene="tolA"
                     /locus_tag="M3X86_RS27170"
                     /old_locus_tag="M3X86_27170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            11905..12270
                     /locus_tag="M3X86_RS27175"
                     /old_locus_tag="M3X86_27175"
     CDS             11905..12270
                     /locus_tag="M3X86_RS27175"
                     /old_locus_tag="M3X86_27175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            12445..12723
                     /locus_tag="M3X86_RS27180"
                     /old_locus_tag="M3X86_27180"
     CDS             12445..12723
                     /locus_tag="M3X86_RS27180"
                     /old_locus_tag="M3X86_27180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     BGC             1268..12798
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5086..5664
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1592..2319)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gaaaaggagg tgaggtgatc gtccgggcta tccagcaact gaaaacattg ccagacattg
       61 gccgcccggt gccgtttctg ccgctggaat ataaggaact ggtgatcggg tttggcgaca
      121 gtggctatgt gatgctctac cgccacgacc gggaaatgga ccagattgtg atcgtcaccg
      181 tcaggcatca gaaagaatcc gggtatccgg gggcagacag cctctaataa ccgatgacgg
      241 gggcgcaatt cattgcgtgg ccgtcgccgg tttgacaagc gcagcgcgtc agaaaaccac
      301 atcctgaacg gaaaaacggg taaaattcag cgatacattt accgtgagaa attacacacc
      361 tttcacaccc gataaaatca ggggtataca gtggcgaaaa cattgctcaa cctttcacag
      421 gcagcacaag ccgcgggcat taccagaaga acgctataca atcatgtaaa acagggaaag
      481 gttaccgtgt cacgtgacgg gaaaaacaac cctgtcgtgg atgtgtcaga actgatccgt
      541 gtatacggaa atgtgaatat ccctgagaaa caaattcccg gcatttcaca ccgtgaaaat
      601 acacaaaaaa atttcccaca ggaacagctt ctggcaatgc aaaaggaact ggccgatctc
      661 cggcaggctg tcaccctgat gctggaagat aaaacatccc gggaagaaga acgccgccag
      721 cacgatgatg agcgccggaa actccaggcc gaagtggaca gattgacaac agaactgaca
      781 cagaagaaaa aacgcttctg gtccggctgg tttagctgag aacttaccga agggaatgga
      841 gaacatgacg aagcaggaaa aaactgccct caatatggct aaattcatta aggatcagtc
      901 gctgctgttg ctggaaaagc tgaacgagct ggacatggat accccggccg acatgtgcga
      961 acggcttcac gaggatgcgg aaacactctg gcaggtgatg cgggataagc tcggggaaga
     1021 ataacagcac cggccatgcc ggtgctggcg ttattttttg ccgaacggcc accaccggcg
     1081 ggcgggtttg tcctccggca gttcaggcac cggcacgtta aaatagcggc tgttatattc
     1141 ccgcatcagc tccgctttca gcttctcgtt caccgcgctt tccccgtgcc gctcgctgac
     1201 cacctcaatc atcgcgttaa tctgctccgg tgagcacttc ccggcatcaa ccacctgcac
     1261 accgttctcg atggcgaacc gctccacctt tcgatagagg taatcacaca gcttcattcc
     1321 tgtgggtttg tcccgttccc attgcggatt ttcaccggtg aaaaagtacg tggctttttc
     1381 gtcgctgttg agccgctcgt catacatccc gatggatttc atcatcgcca gatgctgggc
     1441 ccggtcgaca aaacagaaaa catacgcccc ggtctgccag gcgagcacca ccatgtcttc
     1501 cagatgctcg ctggttggca taatgcagcg ctcgccgtct ttgtcttcca cgtcctcaat
     1561 aaataccatg tacagcgcca taatccctca ctccgttgtc ataaatttaa agcacgcgaa
     1621 cagcctctcc cggtccagct ccaccgtttt gcgggcctcg ccggtctgtg gattcacgat
     1681 ttcatccacc gccagaatcg cccgctgtac ccggacaagc gtcggccggt cagggcagat
     1741 gtaccagatt tcatcgatgc cgttcgcccg gcggttgaac agatacccgg acaccacttc
     1801 ctgataccgc ctgcggctct tgacggtgcg ctctacctca aatgccaccg ttctgccgtc
     1861 cagctccgcc agcgcgtccg gcactttcat ccctttcagg gccatacggt gcaggtaccg
     1921 ccagccggtg gccccccggc cctccatgga gaggcgcagg gactgcacgg ccagccggtg
     1981 cggcagctgc gccgccgaca cccttgacgg ctgaaacgga ataacgtccg tcagcggctc
     2041 gtcctcgtcg aacgagaacg ccaccccgtg cggcgtcagc ccccacacgc tgacctgacg
     2101 gccgtcaacc gggtatttcg cccggaccag cagccctttc gccaccagac tgtccaggaa
     2161 gcggtaaacc ggctgtaccg ccgcaatccc cagccgtcgc gcaaccagct ccgccgtgga
     2221 acacagctct tcccgcagcc agcgcagggt cagccgtgtt ttctccgcgt tccgcgcctg
     2281 ccgcttcgcg tggctgacgt tgtactgctc cagtgtcatc agtaaagccc cgcaaaatca
     2341 tcatccgctt ttggtccgcc gccgtcggcc ggaggcaggt actccgccag cgggtccgtt
     2401 ttcgccagca ccgggaaggt tttcagcgaa atttcccggc gctcaacccg cagcgggctg
     2461 ctgaacgcca gccgcgccac gcccagcccg gtcatcaccg cgaacccgtc aggcaggtgt
     2521 tgcagggtgt tcacgtcaaa cagcggccgc gcctccttct ggatgaagct gtcgccgctg
     2581 atgtgctcca cgttccccgc ctcccggctg gcccggcggc gctccacgtc cacccggatc
     2641 tccccggtct ggcccgccgc ccactgcgcg gactcctggc tggccgcgcg atagaaccag
     2701 cggatcggcg tgttatccag taccgtggtt ttcacaaaat cagcgggcag atcctgcccg
     2761 cactgaccga agtcgcccag cgactggtgc gcgagcagca ggttgcagtc ccggctgcgc
     2821 agcgtcccca gcgcgttcag cacgtacttc gacagcagat atttaatctc atcgagcatg
     2881 atgctcgcgt gcggccacct ccggaactca tcccgcgcaa tgatgatttg cgcgcagcgg
     2941 gcaaacagca tcttctgcac ccggatcacc gcctcatcgt ccatcgagcc gatgacgtac
     3001 aggcagccgc cgccgttgat tatccccgcc acatcgatac cgctgtccgt ctgcaggacg
     3061 ctcaggtcgg ccacgttctc cagcttggtg atcagcccct tcacgtcctt tttcagggcc
     3121 tccggcaacg cgtacgccgc cgccagcacc tgctgaatgt ttgccccctg cgggaactgc
     3181 tccgcaatca gcttcgccgc tttctgctcg ctgatgcggt agtggtccgc cacgtcgccg
     3241 gtctcactca ggttaaaccc ggcaaccagc agattcttca gggcgtactg gtcgagatcg
     3301 cggaacagat tcagctgcgc cggtttcccc ggccgcagat ccagcagggt gaagtccggg
     3361 cagtgcgccc ggaagacgtg cggcgcccac gcgtcccctt tggggtcgaa gtacaccaca
     3421 gcgtccccga aatcgcgtac gcagcgaatg agcgcgttgg tggccatcgc gctcttgccg
     3481 ctcccgggca ggccgaggat ctgaatattg gtcttgcgcc aggtatcacg agggaccagc
     3541 accggctggc cctgctcatc cttacccaga aacaccgcct gctgtttccg ggcctgctcc
     3601 gtggaaaaat cggccagcgc gttaaccggt accggctccc cgaaatgcag gtggcggacg
     3661 tctgtcattt ctccggtccg ctggctgctg cgccggctct cctgattcag tttcgcgttc
     3721 accgcctcgt cagcggccgc aaaatgacca tcaagcagca ggaacgccag cagccgcgcc
     3781 agaaggacgc cggcgacaag ccctccggca caccaggcta tcccgaacag gttagccggc
     3841 tgcagcaggt aataatctgc gttcgcatag aggtccgggc gcaggacggt ttctccccag
     3901 ctctcgataa acacggcgac aaccagcccc cccagaaggg ccagaccggc agcgccggtc
     3961 agccagtacg cgtagttcca gacatgtcgc cgtgccctga ccatccgccc gggtgaatcc
     4021 agcgaggcca gcaccgaaaa tggcacagtc cggttattat cgcgcttata gcgatcggca
     4081 tactcccgcc gcgccttatg gctcagcccc acaaaacgca ttcggccggc ccagagttca
     4141 gccagaccga aaaacaggcc gataaccaga aaaaaaccgg ccagactgta atactcccgg
     4201 tacagccagc tgacccacgg gtaaatctgc cgggccagct ccgggatatg ccactgccag
     4261 gcaagcgaat ccgtattaaa catgcaatct ctctccttca gacgacgggt ttaccggcgt
     4321 gatgcgcagc ccccgctgca caagccgcgg acatgcccca aggcatggcg gcggcggtca
     4381 cgtccgtggt gattgcgttc agctttcacc gcggaggcca gcctctttcc gccccatgcg
     4441 gaaaaggcgc agccccggag gaacaacgag cacgccccag cgtgtgagag gaaaaggcgg
     4501 caccccagtg cggtcttttc cggtgttccg gcccccgtca gagttcgcca tagcgagcgg
     4561 ctgaccaccc gcaaaatcag gagcagcttt gctgcttcgt caggtgcgtc cagcaccgtg
     4621 gatttttgtg ggtggtcagg cgtgagcctg acgggggccg ggtgaatttc tggcgtggcc
     4681 ggcgtccagc cggacagagc cagaaagagg ggaaaagacc gtgcgtcggt ggcgtccagc
     4741 cagcgtaagc aaggtctttt cccctgaagt cagccacgcc ccagcgggga tgacttgcat
     4801 ttctccgcaa ggagaaaaaa gcaacagaga taggttaacg taaaatgttc agcgaaaaac
     4861 cgacatggtt cacctatcct gataattgat cgtcagggaa tagaaagact taatcagggg
     4921 acaatatccc acatcagcgc gtccggtgac cgtcttgcag gtgccgggga aattgcgcct
     4981 cctcgctcac tcggtcgcta cgctccgggc gtgagactgc ggcgggcgct gcggacacat
     5041 acaaagttac ccacagattc cgtggataag caggggacta acatgtgagg caaaacggca
     5101 gggccgcgcc ggtggcgttt ttccataggc tccgccctcc tgccagagtt cacataaaca
     5161 gacgcttttt cggtgcatct gtgggagccg tgaggctcaa ccatgaatct gacagtacgg
     5221 gcgaaacccg acaggactta aagatcccca ccgtttccgg taggtcgctc cctcttgcgc
     5281 tctcctgttc cgaccctgcc gtttaccgga tacctgtccg ccgttctccc ttgcgggaag
     5341 tgtggcgctt tctcatagct cacacactgg tatctcggct cggtgtaggt cgttcgctcc
     5401 aagctgggct gtaagcaaga actccccgtt cagcccgact gctgcgcctt atccggtaac
     5461 tgttcacttg agtccaaccc gggaaagcac gcaaaacgcc actggcagca gccattggta
     5521 actgggagtt cgcagaggat ttgtttagct aaacacgcgg ttgctcttga agtgtgcgcc
     5581 aaagtccggc tacactggaa ggacagattt ggttgctgtg ctctgcgaaa gccagttacc
     5641 acggttaagc agttccccaa ctgacttaac cttcgatcaa accacctccc caggtggttt
     5701 tttcgtttac agggcaaaag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5761 tcttttctac tgaaccgcga tccccgtcag ttcagaggag gacgatggtg ccaccgttcc
     5821 gccctgaaca tcccggatat aatttgcctg acatgcggcc agaagaaagc cagcaacaat
     5881 caatataaaa agaaaaatag tctttacctt tttcatatac tttccctgta tttaaagact
     5941 ccggcattca tccctgaaca ccggaagtca gtatttcaaa tcatccttga taattttttg
     6001 ttaccagtta ccgtcccgat aatcaaaagc tatataacaa ttatccacat caggttttat
     6061 ttgattttta aaaaatggct gtagtaaagg aacccattct ttcttcaaat caaactcacc
     6121 attattaata ttatccttta aaggcattcc caagctttca atgacagaac catcgtcacc
     6181 aaagtcttta gaatactctt cccctttaaa ctcttcagtt ttcttatcaa accatgtcaa
     6241 acgtaattta agccccatat ttacctctca aagatatttt ttaatgttcc gttttggatc
     6301 tggcccttta acctgcttac ccgttttggg atcaaatgcg cctaggtgtt cgccatcact
     6361 ggcacgatac ccttcaagct caccgtgctg ggaatcccat tcataaattt tacgtttttt
     6421 atcgccatac catcgggccc gcttaccacc accaccttgt ttaggtgttt taggatcacc
     6481 tcgctttaac tctccaagtc cctttatgtc ttctgtttta ggtactggat gataatcatg
     6541 gccgtagtct ttagtcccct tgcgaggctt gttcttttcc tcatttaact tattttctgc
     6601 atccttcgct tttttctcct tttgtttccg gctctccatc gccccggtaa gtgcagcctt
     6661 atctctggat aaggattcat tcattgcgtt taaagatgcc tgagccgctt ttaatttctc
     6721 attctgattc tgaatatcct tatcaagata gcgaacctga tcctgccacc cggagccata
     6781 ttcatgaggc gggttataag tcacaaaatc atctttattt gcctctgcct cttttacctt
     6841 tttatcggct tcctgaacag cagcccttgc accggggagc gccttctctg tcgatgcaat
     6901 gcggctgttt aaggtcgcaa tatttttatc ttcggcatcc agctcagctt tcgctttatc
     6961 atagtctctt ttcagcccct cttccggatg agcggcgtcc catgcctgct ggcgacgctt
     7021 ttcttcctcc tgacgctgtt ttacctgtgc cggggtaaga acatctgtca ccgacacata
     7081 aaccggcttc tgtccggtct ctttcgggaa acgaataacg gcctcacggg agttaccacc
     7141 ggctgtaaaa ccagccggac gtgaatcacc tttttcagca acaatgcctt ttggcggggc
     7201 tttcgctgtc ggaacacctt tcggtacgct cacctgcaga gacgggagac ccggaatcga
     7261 cacactgaat acccccggac gttttgtcgg tttcgcatcc accacaggga cactcattgg
     7321 ccgacctgcg acaaccgcaa tatgctgccg ctcatccttc accacatcca caacccgttg
     7381 tctgacacga accgtcgcct ggtccagtgg taaagtactt gccggagtct ccgtcaccgc
     7441 atcggccggt aatgacgtca taatttttga catcattttc ggatcatctt ttgcaatctc
     7501 agaaggcagc acaccgtaga tcgcaatccc ccacagacca aacttaaacg gccccttcag
     7561 ggcagccagc acatcagcaa cggcggccga caacgcatcg ccggaaactg ataaagccag
     7621 tccttcagcc cccggagtag ccagggcagg cagaccgaag gccatcgcag tggccgaaga
     7681 ctgtccgcca ccagagctac cgctatgatt gccgctgtta ccaccattac caccgggttt
     7741 actcccgccg ttaccaaact cccccggatt atagttatga atatctccat tcggcgtatg
     7801 cgtcgtaccc cagcccggcc cgctgtttgg atccgtacca ccacctgatg aagggccacc
     7861 tttaccagac gtaccgttca cattcccact ggcctgacca ccattatgtc ccagacctga
     7921 attacccgga cctcgaccgt ctccaccact cataccgttt tcctctttga catttaaaac
     7981 aataaattaa agccaacatg tcgcggtagg gatacccgtt accggatacc ccccgcacag
     8041 atcccggcgt gcgcgattta cgcaccgggc tcctgcctcg ggtgtctggc ggtgaaccgc
     8101 tccacaggcc atggatgaag aacccgaacc cttggtagcc atgcggctgc cagtttgttt
     8161 gctttcgtcc aggtcgtatc atccttctgg ctcctgcgcc tgagcgcccg gcgccagagg
     8221 tttgttacgt gtgtcctgaa cttctgcatg gtggggaagt tgcccggtac cgagtgatag
     8281 ttcaggtatc cctgaaccac tctcctgagc cattttccct gttcggggat tgagtaatgc
     8341 cagcgccttc gcagaccgtc tttgatggct ttcagagttg ccgtcatccg atcccggcgg
     8401 gtctttcgta tcagcatgaa cctgccgttg cgatctttcc cgctgatgtg cgtgaacccg
     8461 aggaagttga acgtttctgg tttgcctttt cccctgatgg cacggttttc ggcagcgaag
     8521 cggccgaact ccatcagacg ggttttctcc gggtgaaccg tgagtccgaa ctccctcagt
     8581 ctgcgctgca tggctatacg gaagcgccgg gcatcgtatc gtttgtcgaa cccgatgacg
     8641 atgtcatcgg cgtatctgac cattaccaca ttgcctgtgg catagcgacg tcgccactga
     8701 tgcgcccaca gatcgaagac gtagtggagg tatatgtttg ccagcagcgg tgagatgacc
     8761 gcaccctgtg gggtgccttc ctccgttgct cgccattgac cctcctccga cgtcccggct
     8821 gtgagccact tacgtatgag cctgattacc ctccggtcgc cgatccgatg ctctgtgaac
     8881 ctgatcagcc attcgtggct caccctgtcg aagaactgac tgatgtcggc atccagtacc
     8941 cagtttacgt tagtgcgtac cagccctgtg gccagtgcgt ccagtgcatc gtgctggctt
     9001 cgcccgggtc tgaacccgta tgagaacccc ataaagtcgt tttcatagac tgcgttcagg
     9061 attttcacca gcgcatactg gacgatcttg tcctccagcg aggcgatgcc gagcgggcgt
     9121 tgttttccat ccgcttttgg gatgtagtga cgcctgccgg gctgcgccct gtagctgccc
     9181 tgatgtagcc tccggggcac tgttgcaaat agtcggtggt gataaactta tcatcccctt
     9241 ttgctgatgg agctgcacat gaacccattc aaaggccggc attttcagcg tgacatcatt
     9301 ctgtgggccg tacgctggta ctgcaaatac ggcatcagtt accgtgagct gcaggagatg
     9361 ctggctgaac gcggagtgaa tgtcgatcac tccacgattt accgctgggt tcagcgttat
     9421 gcgcctgaaa tggaaaaacg gctgcgctgg tactggcgta acccttccga tctttgcccg
     9481 tggcacatgg atgaaaccta cgtgaaggtc aatggccgct gggcgtatct gtaccgggcc
     9541 gtcgacagcc ggggccgcac tgtcgatttt tatctctcct cccgtcgtaa cagcaaagct
     9601 gcataccggt ttctgggtaa aatcctcaac aacgtgaaga agtggcagat cccgcgattc
     9661 atcaacacgg ataaagcgcc cgcctatggt cgcgcgcttg ctctgctcaa acgcgaaggc
     9721 cggtgcccgt ctgacgttga acaccgacag attaagtacc ggaacaacgt gattgaatgc
     9781 gatcatggca aactgaaacg gataatcggc gccacgctgg gatttaaatc catgaagacg
     9841 gcttacgcca ccatcaaagg tattgaggtg atgcgtgcac tacgcaaagg ccaggcctca
     9901 gcattttatt atggtgatcc cctgggcgaa atgcgcctgg taagcagagt ttttgaaatg
     9961 taaggccttt gaataagaca aaaggctgcc tcatcgctaa ctttgcaaca gtgccgcctc
    10021 cggtgcagat ctgttatgtt gttcttcatg tttccggcgt agtccatcca cctgatgcca
    10081 tccactccgg cggccgcttt cctgctcagg gagaggaatg cggcttccag tgcttcgact
    10141 gtcagcaggt ggaacaatgc tgtaaaccgt tctttcttcc gctgcttcgc agcttcccgc
    10201 acgcgtgaca gcctctgtga catgctttcc cggctctgtg tccggcgcat gtgtggctgt
    10261 tccgcgttcc ccttggcccc gctccttcgc tccactgact ccgctccttt cgggttgttc
    10321 gcctgcttcg ccgctactat gagcgagtcc gacttctcct ctccgtacat caccggctat
    10381 gactcctcgt cttcccggtg cgggccatct ccgacactgg cagatggtca gaggggagat
    10441 ctcccggttc ccgcgtagag atcgtattga catgccaggg tctcagaccc cgccgggtcc
    10501 atgtggcact cgcagtatcg caccctatga tgttgccttc cgttaacagt acaacgtcgg
    10561 cacccggtaa tttaatatac atttcgtggc tcaatggctg gcctgtcaac acccctgtca
    10621 acgcttcgcc ccatacctcg cggtatgcaa cgcatgactc ggggaccttg tggattgctg
    10681 gtccttcaat ggtcggggac tttcacccct tgatctctaa ccggtctccc ggcgcacact
    10741 gtacatatac acagtataat tattacctta ctcctcccgt tttcaggtgt caatatatga
    10801 gattagttct catcttcagg cacaaaaaag cgcccggagc cacctgtaag cagatcgcgt
    10861 cttttcccga aaatttctca tggcaacaaa ttttattatt taagacattc atcacactta
    10921 catttcagat ggtatagaca catctgaaag ctaactggca tgaggagagg acctgcggtt
    10981 aaactgttcg ggaaacctga gacgcccgga ggccactatg acgcacctga aactgactcc
    11041 cgctgaagaa aagcgcctgg cggacgcctt tgtcgctcag gcactggcca tgggcctgaa
    11101 agggaacaaa acgctgccaa aggcggtaaa acaacaggtg cgggaaaggg tgcttcgtgc
    11161 cattcaggac gaacgtgaag ccaggcgggc gcaggaaatg cgtcgggaga aatacgaggc
    11221 tacggaaacc accttcacct ggcagccaca gcgccgccgg taaggggtat ttaccataac
    11281 gtcgcattgc ccgcaccgct cagacggtct gagggcgttt ccggcggcta cgggataaaa
    11341 caggcaacaa accacccgga aaaccgccgg aaacgcgccc ggtaaattgt aacccgctgc
    11401 atgcctatgc acgcaactgc atggcgggcc gtttggtgta aaaacgatac gataagccgt
    11461 gtttatgtaa atgaacgtgg ccgtaaagag tgacaaatat acatgtaaca ttttttactg
    11521 acaggtcatc aacccgatgc gtttaatacc ctttatcctg ctggcattgt gcagcccctt
    11581 actcctgacg gcctgtaagc attccaccac ccagccgccg aaaatttata ctctggataa
    11641 gtcatctaaa atcagtagct acgctgcggc catcaagacc gctgttgaaa tgcagatgtt
    11701 tgaaccagag gcctatgcgg gaaaaatgtg caccgtgcgg atatccatac aacgtgatgg
    11761 cactctgaac agcgccattg ctgaaggagg agatccgaaa ctctgtaaag cagcaatatc
    11821 agccgttacc cacgccaaaa ttccgccggc accggatgaa aatacctggc aaatattcaa
    11881 aaacgcccct ctggatttca gaccatgaaa gcttttactc tgacagtgct ggcggccatg
    11941 ttcctcactc cggtacatgc gataccacta aaacccgtaa ccacactgca gccaacctac
    12001 ccggtacagg ccgcagcaaa acgcatcgaa ggtcaggtga ccgccacgtt tgatattctg
    12061 gacgatggac gagtggacaa cgtgtcgctt accggctgtc accttttttt tcgggaaacg
    12121 ctgacggcaa tgaggcactg gctatatccc gcagggcacc cggcaatgcg acaggttata
    12181 aattttgaat tcagtctgaa aaatgacggc ttacaggatc cgcgaccagt caccacccaa
    12241 aagctcaatg atgatggttg ccgccagtaa caggtccttt aacccgcagg ctgcaatagt
    12301 cctggatgat gagtgagcct gtagcagccc tgattagtgg aagcgaagct gaaaaatggg
    12361 cagcaaaagc cgtatttctg gcaaatagcg ccgccacgga ttaaagtagt catgtgtaat
    12421 acatatgact acaaaggggc ttttatgtca tcagcaacat ccatcaggct cgatgaagaa
    12481 cttaaggacc ggctgaaaac gctggccgat gaccggcacc gctcagcgca cgcgctaatg
    12541 ctggaggcaa tcaccgaata catcgaccgg gaagagaagc gcagccagta cctgcgggac
    12601 ggacaggccg catggcagca ctatcaggaa acgggtctgc accttactgc ggaagaagcg
    12661 gaagcctgga taagtacctg gggaactgag aacgagcagg atgctccgcc atgccacagg
    12721 taacgatttc cgcgctggca cagcgcgatt tacaacgcct tcaggacttt ctcaaaacca
    12781 aaaatcggct ggcagcca
//
