LOCUS       NZ_CP097631             5595 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Klebsiella pneumoniae strain 140253 plasmid p4_140253, complete
            sequence.
ACCESSION   NZ_CP097631
VERSION     NZ_CP097631.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28546263
            Assembly: GCF_023572965.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Wen,H., Zhao,F., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain 140253
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Wen,H., Zhao,F., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2022) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. 0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023572965.1-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 11:11:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,930
            CDSs (total)                      :: 5,807
            Genes (coding)                    :: 5,653
            CDSs (with protein)               :: 5,653
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 56 of 154
            Pseudo Genes (incomplete)         :: 102 of 154
            Pseudo Genes (internal stop)      :: 17 of 154
            Pseudo Genes (multiple problems)  :: 21 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097631.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="140253"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4_140253"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2020-08-22"
     gene            join(5167..5595,1..117)
                     /locus_tag="M9P58_RS29435"
                     /old_locus_tag="M9P58_29435"
     CDS             join(5167..5595,1..117)
                     /locus_tag="M9P58_RS29435"
                     /old_locus_tag="M9P58_29435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            183..533
                     /locus_tag="M9P58_RS29440"
                     /old_locus_tag="M9P58_29440"
     CDS             183..533
                     /locus_tag="M9P58_RS29440"
                     /old_locus_tag="M9P58_29440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(603..869)
                     /locus_tag="M9P58_RS29445"
                     /old_locus_tag="M9P58_29445"
     CDS             complement(603..869)
                     /locus_tag="M9P58_RS29445"
                     /old_locus_tag="M9P58_29445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(853..1077)
                     /locus_tag="M9P58_RS29450"
                     /old_locus_tag="M9P58_29450"
     CDS             complement(853..1077)
                     /locus_tag="M9P58_RS29450"
                     /old_locus_tag="M9P58_29450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(1744..1956)
                     /locus_tag="M9P58_RS29455"
                     /old_locus_tag="M9P58_29455"
     CDS             complement(1744..1956)
                     /locus_tag="M9P58_RS29455"
                     /old_locus_tag="M9P58_29455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1963..2472)
                     /locus_tag="M9P58_RS29460"
                     /old_locus_tag="M9P58_29460"
     CDS             complement(1963..2472)
                     /locus_tag="M9P58_RS29460"
                     /old_locus_tag="M9P58_29460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2507..3211)
                     /locus_tag="M9P58_RS29695"
                     /pseudo=""
     CDS             complement(<2507..3211)
                     /locus_tag="M9P58_RS29695"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3150..3494)
                     /locus_tag="M9P58_RS29470"
                     /old_locus_tag="M9P58_29470"
     CDS             complement(3150..3494)
                     /locus_tag="M9P58_RS29470"
                     /old_locus_tag="M9P58_29470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3484..3660)
                     /locus_tag="M9P58_RS29475"
                     /old_locus_tag="M9P58_29475"
     CDS             complement(3484..3660)
                     /locus_tag="M9P58_RS29475"
                     /old_locus_tag="M9P58_29475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4300..4458
                     /locus_tag="M9P58_RS29700"
     CDS             4300..4458
                     /locus_tag="M9P58_RS29700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4815..5141
                     /locus_tag="M9P58_RS29480"
                     /old_locus_tag="M9P58_29480"
     CDS             4815..5141
                     /locus_tag="M9P58_RS29480"
                     /old_locus_tag="M9P58_29480"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     Typing          complement(3747..3823)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          4012..4409
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(1629..3160)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gagggtgaag cagctgcttt catggatgtt cttaaacaat tattattatt tgaggataat
       61 tttcgccttt ttggtagaga cttggtttat aaaatagaaa aattaaaaat cagataacaa
      121 agtatggtga acgaaaaatg aaagagagga tagtgaaatg tgtaatacca agattctcaa
      181 ctatgtattg gttaattgcg ttcctagcat ttatattccc cggtcgatct tttgctgacg
      241 gttacaccgc ttactaccga tgtaatggta ctcaggaagc atctcttact gttaaagata
      301 acaaactaag tatggttgta ggtctttcta agtttaatag gcaactggat acttatagat
      361 tcaaagaaaa gggaatcgaa gtagtaatgc agagagctgc ttctgatgat aactctgagg
      421 ccataatgtc ttttattatg cctgacgtta acaatatgac cttattagtt aaaaataacg
      481 gctacattat cgttaagaca acctgctggt taactaaaac cgacaaacag tagaacacgt
      541 agttagtcga gtatttacca taatacctgt tgtcggcatc aaaaaagccc gcatagcggg
      601 cttcagtcgt aaacttcgcg gcgatgtcca atcgtggcgg caaggataac aagctcgtca
      661 tctcggatct cacacagaac gcgataatct cccacccgat agcgccagaa ctcgcccagc
      721 tcacctttga gcggcttccc tgactggcga ggatcggcag caactgcaat gcgtaaactc
      781 ataaaatcca caatccgtcg tgcgttctgt ttgtccatct tgcgtaacga tttgagcgcc
      841 cgatcggaat agttaatcgt ccagaccaag tgacttcctc acgtcttcag acgaatgcac
      901 tgcttcatca ccacggcgaa cgcgtgcagc agtttctgct gaaagataat aatcctccag
      961 gtcttccaga tgcgccagta ttgcttcccg cgcataaaac gtctttgttc tgccggtttg
     1021 cttcgccagc gagtccagac gggactcaat ctcatcagat aaccggatag caagcatata
     1081 aacctcccga acaccatttg ctatacatgt atatcaacag taccacaatc aacctaaaag
     1141 aacaaaaaat aatcttgtta gcccctcttg ataatccgta acgcggtcaa tatatctagc
     1201 ttgagggctt tcctcttaac tattgaaagg ataacgatta tgggtcttaa gccgggtccg
     1261 aaacgaattg ccgattcaac aggggaacct gataaacgcc agcgtgacaa taaaaaaacg
     1321 ccgggaaata ctgacaaatt gaaacccagc aaatcttcaa aaaaataagt cagtagttaa
     1381 aagccctccg gggctttttt gtatctgccg ttcacgctga ttaaacataa cacccgttgc
     1441 ccgcaccgcc ccggacgggt tcgccgcgaa cggggtggca gtcaggtaaa accggcaaca
     1501 aaccacccga taaaccgccc cgttcgcgac agataaattt tacctggctg catagccatg
     1561 cagccaggtg aatgacatcc ggtttcagcg taaatgcccg atgagaaccc tgttttcctg
     1621 catttcagcc cagcgacgga ccgtcatagc ttcgctgccg ggaacgcgct tccgtcagtt
     1681 ccttttcgtg ctgccgttgc tcgtgcgccc gttctgcggc cacggccatc tcatgacgct
     1741 gcactaccgg ctcaaacgct gcagttgccc gctcaagcgt tccacctgct gactcaagcg
     1801 cgtcacgcgc tcgctcagcg ccgcgttctc ccgttgcata agccctgaca ttttccgcca
     1861 ttccccgaag gcgctctccc actcgtccag ccttttcgag tagtcctgct gtagctgctc
     1921 taatgcgctc agcaactgtt tttccagctc ggtcatcggc acttactcct tcaccagtct
     1981 cagcgcgttc tgcttcgacc gcccgttccc gaccgtccag ctctgcgtcc tgtctacggc
     2041 cgtccccttc ggcagcacca gaaaacgtcc gttgctgtct tccaggtacg tcacgcccca
     2101 ggtcttcgcg ttcagcttcg acagcgctgc gttctgccgg tcgatttcgg ccagatttga
     2161 cgctatcaag ctcccctgat accacaacac cccgctcagg ctggcgaaca gcagtcctga
     2221 caccatcgtg atggtcagcc atgtccgccc gaccatacgc atcacgctca ggcggttcga
     2281 ctgcatggcc gcgctcatac cctgctcgtg ggcgcggatg gcggcgctga ttctcttccc
     2341 gctttcgctc agttccgctc tgacagactt ctcgtgctcg ctgaatgcgg ctttcagcat
     2401 ttcgccggta ctctgctgct gcgctttcga tttctgctct aagtccttcg ccagcgttaa
     2461 aagactgttc atagatggct cccttaagtc ggatgtttcg ccctccgtcc gggtcggcaa
     2521 tgctgatgct gctctttgtg gtgcgcacga cctcaaaacc tgcgctttcc agcgcctcag
     2581 tgacgtcctg acgcgtttta agctcccctg acgaggcgag ggccagtaag ccccgcgtaa
     2641 tagcctctgc ggcttcctgc ttcgttttgg gtaacgaaga tggcgtcacc agcgcgcgcc
     2701 ggttctccgg tgcgttcggg tcgtgcagcc ccagtcgccc gttcaccacg gtctgccacg
     2761 catcgatgcg cggacggtct gcccggtcgt agtacggctg gagacgcctg ccggtcagca
     2821 gttccgtgtt cggtaccaga aaattcagtt ccagccgccc cttgtcccgg tgttcaaccc
     2881 acagcacgct gtactggtct ttatcgagtc cgggcatcag aacccgctcg aagctcgcca
     2941 tcagcttttc acgctggccg ggcggtaaat cctgttcagc aaaagacagg acgccggagg
     3001 tgtacttttt ggcgtagggc gaggcgtcga taagctcccg gacctcctcc ggctttccct
     3061 gcaggacgat ggccccgtcg cgctggcggt ctttccccag cagataatcc accggcccgg
     3121 caccgccgcc tcgccctcgc ggatgaaact taacgatcat cgtctgcccc cttttccagc
     3181 acggcatgcc gcagccgctc gagttcggca tcaatggcca tcagcgcggc caccacctga
     3241 acccggtcag cgcctgacca ctggccgccg ttaattttcc gggcgatctg gttgaggttg
     3301 ttccccatcc cggcaagctg gcgcagcaaa accggatcga tggacggcag ccgcaacgac
     3361 cgcgccgggc gcgtatcgag acaggtttcc cgcatccaca ccgccagttg ccttccattg
     3421 cagcgctcca gcagctgctg gtactcccca tcggtcaccc ggatacctaa aaaacgggta
     3481 cgcttaaccg ccatcctctc ctccgatatc cgcaaaatgt gaccgggctg aacgctcaag
     3541 ctctgccatc tgctcccggc ggtaatcttc ctcctgtgcc tgacgcatca gatttgccat
     3601 cactgccgga tgccaggatc ttttttttac gacgagcggc ttcttcttcg gcttctgcat
     3661 tatccctcct tcgcaaacgc cggacggcac ggaaaagttt tccgggtcgc tccggcgttg
     3721 gacaagcgcc agcgcgtcag tggtgttagc gggtttcggg gcgcagccct gaaccagtca
     3781 cagcgtttac gctgtgtata ctggcttact atgttggcat gagagcacga ttcatgaaag
     3841 tgcctcatgt ggcaggtgtg atatgtggct caggagcgtc cgcaatacca tgcacggaca
     3901 gatgaaatga cgcctcctcg ctcactcggt agctgcgctc ctgccgttcg gctgcggcgg
     3961 gcgtgaccga ctcacggata acgggataaa gcaaaggcgc tcaaatcaga aagggccgga
     4021 gaaaaggatg cggcgttgcc gtttttccat aggctccgcc tcccctgacg agcatcacag
     4081 aaatggacgc tcaagtcaga ggaggacgaa acccgacagg acttaaagat gccggtgatt
     4141 tctctccggc ggctccctcg tgcgctctcc tgttccgacc ctgccgttta ccggatacct
     4201 gttccgcctt tttccctttc gggaagcgtg gcgctttctc atagctcacg ctgctggtat
     4261 ctcggctcgg tttaggtcgt tcgctccaag ctgggctgta tgcacgaacc ccccgttcag
     4321 cccgactgct gcgccctttc cggtaactat catcttgagt ccaacccgga cggacacgca
     4381 aaaacgccac tggcagcagc cattggtaac tggatatata cgagagagat attgagaggt
     4441 cttgaagtgg gtgcctaact gcggctacac tgaaagaaca gttttggtat ctgcggtctc
     4501 gtatacccag ttacccggtt aagcagttcc ccaactgact taaccttcga tcaaaccgcc
     4561 tccccaggcg gttttttcgt ttacagtgca agagattacg cgcagaaaaa aaggatctca
     4621 agaagatcct ttaaatgatc gtcagttctg ttatccccgg agctccatcc atcccacgga
     4681 aaaacacccg ccactgatgc cctcagcgca ctgcacatca tgtacaaacc actccctccc
     4741 tcctttttta ttgcgtttct taatgcgcaa tggtagcttt gtgcgcaaca aaaaacgcaa
     4801 tagcaagggg ggtaatgaga gaaagaacaa atatcatgct agatatagga ttaaagcaat
     4861 ccttacaacg gctttcgaat aaaaccaaaa tgagtctaag tgatcttatt aaccagagat
     4921 tatcaggttc actttcagtt gaagaacgca ttggcgaaaa gcctgaatgg atgagaaacc
     4981 ttgatcccta tctcgccaat cacttttcaa atattgagtt accgctccct tcaccattaa
     5041 aagaatacca agaaaaaatg gatgagatag attcaattat taatgacatg atggttctaa
     5101 aggaatattt cagagctaag ttttacgacg aaataaaata ggcacgctat acggaggcgg
     5161 agaattatgt tttttaaacg gtatttaacg gagaaggaaa tatacaaggc agcactccca
     5221 attgttaggc ttcaagggag agttaacact tcattacgtt ttttatccaa atactcagat
     5281 gtaaacaaaa atggattaat tgcattgcat cttaatttat tacattttat ctcatacaac
     5341 atacacggtg tagagtctct gaaaatcact caagcaggca taaacaaagc tgtacaacaa
     5401 agccctcacg cagatgaaat aaaaaatatt gcgcaggcaa taggacgcaa aggacctgag
     5461 tacatcgaag gattaattag aaacccatgg gggaagaaaa atgataatcc actaatattt
     5521 ataatgagca ttgaagaaag tataaaagaa acccttaact caggttatat gttatataga
     5581 gtagctccca taact
//
