LOCUS       NZ_CP097630            10060 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Klebsiella pneumoniae strain 140253 plasmid p3_140253, complete
            sequence.
ACCESSION   NZ_CP097630
VERSION     NZ_CP097630.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28546263
            Assembly: GCF_023572965.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Wen,H., Zhao,F., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain 140253
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Wen,H., Zhao,F., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2022) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. 0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023572965.1-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 11:11:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,930
            CDSs (total)                      :: 5,807
            Genes (coding)                    :: 5,653
            CDSs (with protein)               :: 5,653
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 56 of 154
            Pseudo Genes (incomplete)         :: 102 of 154
            Pseudo Genes (internal stop)      :: 17 of 154
            Pseudo Genes (multiple problems)  :: 21 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097630.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="140253"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3_140253"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2020-08-22"
     gene            complement(join(9687..10060,1..1591))
                     /locus_tag="M9P58_RS29370"
                     /old_locus_tag="M9P58_29370"
     CDS             complement(join(9687..10060,1..1591))
                     /locus_tag="M9P58_RS29370"
                     /old_locus_tag="M9P58_29370"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3075..3224)
                     /locus_tag="M9P58_RS29375"
                     /old_locus_tag="M9P58_29375"
     CDS             complement(3075..3224)
                     /locus_tag="M9P58_RS29375"
                     /old_locus_tag="M9P58_29375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3309..3566)
                     /locus_tag="M9P58_RS29380"
                     /old_locus_tag="M9P58_29380"
     CDS             complement(3309..3566)
                     /locus_tag="M9P58_RS29380"
                     /old_locus_tag="M9P58_29380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3576..5261)
                     /locus_tag="M9P58_RS29385"
                     /old_locus_tag="M9P58_29385"
     CDS             complement(3576..5261)
                     /locus_tag="M9P58_RS29385"
                     /old_locus_tag="M9P58_29385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5588..5833
                     /locus_tag="M9P58_RS29390"
                     /old_locus_tag="M9P58_29390"
     CDS             5588..5833
                     /locus_tag="M9P58_RS29390"
                     /old_locus_tag="M9P58_29390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6107..6478
                     /gene="tolA"
                     /locus_tag="M9P58_RS29395"
                     /old_locus_tag="M9P58_29395"
     CDS             6107..6478
                     /gene="tolA"
                     /locus_tag="M9P58_RS29395"
                     /old_locus_tag="M9P58_29395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            6475..6840
                     /locus_tag="M9P58_RS29400"
                     /old_locus_tag="M9P58_29400"
     CDS             6475..6840
                     /locus_tag="M9P58_RS29400"
                     /old_locus_tag="M9P58_29400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            7015..7293
                     /locus_tag="M9P58_RS29405"
                     /old_locus_tag="M9P58_29405"
     CDS             7015..7293
                     /locus_tag="M9P58_RS29405"
                     /old_locus_tag="M9P58_29405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            7281..7595
                     /locus_tag="M9P58_RS29410"
                     /old_locus_tag="M9P58_29410"
     CDS             7281..7595
                     /locus_tag="M9P58_RS29410"
                     /old_locus_tag="M9P58_29410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7759..8187
                     /locus_tag="M9P58_RS29415"
                     /old_locus_tag="M9P58_29415"
     CDS             7759..8187
                     /locus_tag="M9P58_RS29415"
                     /old_locus_tag="M9P58_29415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            8213..8392
                     /locus_tag="M9P58_RS29420"
                     /old_locus_tag="M9P58_29420"
     CDS             8213..8392
                     /locus_tag="M9P58_RS29420"
                     /old_locus_tag="M9P58_29420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8419..8949)
                     /locus_tag="M9P58_RS29425"
                     /old_locus_tag="M9P58_29425"
     CDS             complement(8419..8949)
                     /locus_tag="M9P58_RS29425"
                     /old_locus_tag="M9P58_29425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8956..9687)
                     /gene="mobC"
                     /locus_tag="M9P58_RS29430"
                     /old_locus_tag="M9P58_29430"
     CDS             complement(8956..9687)
                     /gene="mobC"
                     /locus_tag="M9P58_RS29430"
                     /old_locus_tag="M9P58_29430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2394..2972
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8960..9687)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 agaaccagcg gatcggcgtg ttatccagta ccgtggtttt cacaaaatca gcgggcagat
       61 cctgcccgca ctgaccgaag tcgcccagcg actggtgcgc gagcagcagg ttgcagtccc
      121 ggctgcgcag cgtccccagc gcgttcagca cgtacttcga cagcagatat ttaatctcat
      181 cgagcatgat gctcgcgtgc ggccacctcc ggaactcatc ccgcgcaatg atgatttgcg
      241 cgcagcgggc aaacagcatc ttctgcaccc ggatcaccgc ctcatcgtcc atcgagccga
      301 tgacgtacag gcagccgccg ccgttgatta tccccgccac atcgataccg ctgtccgtct
      361 gcaggacgct caggtcggcc acgttctcca gcttggtgat cagccccttc acgtcctttt
      421 tcagggcctc cggcaacgcg tacgccgccg ccagcacctg ctgaatgttt gccccctgcg
      481 ggaactgctc cgcaatcagc ttcgccgctt tctgctcgct gatgcggtag tggtccgcca
      541 cgtcgccggt ctcactcagg ttaaacccgg caaccagcag attcttcagg gcgtactggt
      601 cgagatcgcg gaacagattc agctgcgccg gtttccccgg ccgcagatcc agcagggtga
      661 agtccgggca gtgcgcccgg aagacgtgcg gcgcccacgc gtcccctttg gggtcgaagt
      721 acaccacagc gtccccgaaa tcgcgtacgc agcgaatgag cgcgttggtg gccatcgcgc
      781 tcttgccgct cccgggcagg ccgaggatct gaatattggt cttgcgccag gtatcacgag
      841 ggaccagcac cggctggccc tgctcatcct tacccagaaa caccgcctgc tgtttccggg
      901 cctgctccgt ggaaaaatcg gccagcgcgt taaccggtac cggctccccg aaatgcaggt
      961 ggcggacgtc tgtcatttct ccggtccgct ggctgctgcg ccggctctcc tgattcagtt
     1021 tcgcgttcac cgcctcgtca gcggccgcaa aatgaccatc aagcagcagg aacgccagca
     1081 gccgcgccag aaggacgccg gcgacaagcc ctccggcaca ccaggctatc ccgaacaggt
     1141 tagccggctg cagcaggtaa taatctgcgt tcgcatagag gtccgggcgc aggacggttt
     1201 ctccccagct ctcgataaac acggcgacaa ccagcccccc cagaagggcc agaccggcag
     1261 cgccggtcag ccagtacgcg tagttccaga catgtcgccg tgccctgacc atccgcccgg
     1321 gtgaatccag cgaggccagc accgaaaatg gcacagtccg gttattatcg cgcttatagc
     1381 gatcggcata ctcccgccgc gccttatggc tcagccccac aaaacgcatt cggccggccc
     1441 agagttcagc cagaccgaaa aacaggccga taaccagaaa aaaaccggcc agactgtaat
     1501 actcccggta cagccagctg acccacgggt aaatctgccg ggccagctcc gggatatgcc
     1561 actgccaggc aagcgaatcc gtattaaaca tgcaatctct ctccttcaga cgacgggttt
     1621 accggcgtga tgcgcagccc ccgctgcaca agccgcggac atgccccaag gcatggcggc
     1681 ggcggtcacg tccgtggtga ttgcgttcag ctttcaccgc ggaggccagc ctctttccgc
     1741 cccatgcgga aaaggcgcag ccccggagga acaacgagca cgccccagcg tgtgagagga
     1801 aaaggcggca ccccagtgcg gtcttttccg gtgttccggc ccccgtcaga gttcgccata
     1861 gcgagcggct gaccacccgc aaaatcagga gcagctttgc tgcttcgtca ggtgcgtcca
     1921 gcaccgtgga tttttgtggg tggtcaggcg tgagcctgac gggggccggg tgaatttctg
     1981 gcgtggccgg cgtccagccg gacagagcca gaaagagggg aaaagaccgt gcgtcggtgg
     2041 cgtccagcca gcgtaagcaa ggtcttttcc cctgaagtca gccacgcccc agcggggatg
     2101 acttgcattt ctccgcaagg agaaaaaagc aacagagata ggttaacgta aaatgttcag
     2161 cgaaaaaccg acatggttca cctatcctga taattgatcg tcagggaata gaaagactta
     2221 atcaggggac aatatcccac atcagcgcgt ccggtgaccg tcttgcaggt gccggggaaa
     2281 ttgcgcctcc tcgctcactc ggtcgctacg ctccgggcgt gagactgcgg cgggcgctgc
     2341 ggacacatac aaagttaccc acagattccg tggataagca ggggactaac atgtgaggca
     2401 aaacggcagg gccgcgccgg tggcgttttt ccataggctc cgccctcctg ccagagttca
     2461 cataaacaga cgctttttcg gtgcatctgt gggagccgtg aggctcaacc atgaatctga
     2521 cagtacgggc gaaacccgac aggacttaaa gatccccacc gtttccggta ggtcgctccc
     2581 tcttgcgctc tcctgttccg accctgccgt ttaccggata cctgtccgcc gttctccctt
     2641 gcgggaagtg tggcgctttc tcatagctca cacactggta tctcggctcg gtgtaggtcg
     2701 ttcgctccaa gctgggctgt aagcaagaac tccccgttca gcccgactgc tgcgccttat
     2761 ccggtaactg ttcacttgag tccaacccgg gaaagcacgc aaaacgccac tggcagcagc
     2821 cattggtaac tgggagttcg cagaggattt gtttagctaa acacgcggtt gctcttgaag
     2881 tgtgcgccaa agtccggcta cactggaagg acagatttgg ttgctgtgct ctgcgaaagc
     2941 cagttaccac ggttaagcag ttccccaact gacttaacct tcgatcaaac cacctcccca
     3001 ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga aaaaaaggat ctcaagaaga
     3061 tcctttgatc ttttctactg aaccgcgatc cccgtcagtt cagaggagga cgatggtgcc
     3121 accgttccgc cctgaacatc ccggatataa tttgcctgac atgcggccag aagaaagcca
     3181 gcaacaatca atataaaaag aaaaatagtc tttacctttt tcatatactt tccctgtatt
     3241 taaagactcc ggcattcatc cctgaacacc ggaagtcagt atttcaaatc atccttgata
     3301 attttttgtt accagttacc gtcccgataa tcaaaagcta tataacaatt atccacatca
     3361 ggttttattt gatttttaaa aaatggctgt agtaaaggaa cccattcttt cttcaaatca
     3421 aactcaccat tattaatatt atcctttaaa ggcattccca agctttcaat gacagaacca
     3481 tcgtcaccaa agtctttaga atactcttcc cctttaaact cttcagtttt cttatcaaac
     3541 catgtcaaac gtaatttaag ccccatattt acctctcaaa gatatttttt aatgttccgt
     3601 tttggatctg gccctttaac ctgcttaccc gttttgggat caaatgcgcc taggtgttcg
     3661 ccatcactgg cacgataccc ttcaagctca ccgtgctggg aatcccattc ataaatttta
     3721 cgttttttat cgccatacca tcgggcccgc ttaccaccac caccttgttt aggtgtttta
     3781 ggatcacctc gctttaactc tccaagtccc tttatgtctt ctgttttagg tactggatga
     3841 taatcatggc cgtagtcttt agtccccttg cgaggcttgt tcttttcctc atttaactta
     3901 ttttctgcat ccttcgcttt tttctccttt tgtttccggc tctccatcgc cccggtaagt
     3961 gcagccttat ctctggataa ggattcattc attgcgttta aagatgcctg agccgctttt
     4021 aatttctcat tctgattctg aatatcctta tcaagatagc gaacctgatc ctgccacccg
     4081 gagccatatt catgaggcgg gttataagtc acaaaatcat ctttatttgc ctctgcctct
     4141 tttacctttt tatcggcttc ctgaacagca gcccttgcac cggggagcgc cttctctgtc
     4201 gatgcaatgc ggctgtttaa ggtcgcaata tttttatctt cggcatccag ctcagctttc
     4261 gctttatcat agtctctttt cagcccctct tccggatgag cggcgtccca tgcctgctgg
     4321 cgacgctttt cttcctcctg acgctgtttt acctgtgccg gggtaagaac atctgtcacc
     4381 gacacataaa ccggcttctg tccggtctct ttcgggaaac gaataacggc ctcacgggag
     4441 ttaccaccgg ctgtaaaacc agccggacgt gaatcacctt tttcagcaac aatgcctttt
     4501 ggcggggctt tcgctgtcgg aacacctttc ggtacgctca cctgcagaga cgggagaccc
     4561 ggaatcgaca cactgaatac ccccggacgt tttgtcggtt tcgcatccac cacagggaca
     4621 ctcattggcc gacctgcgac aaccgcaata tgctgccgct catccttcac cacatccaca
     4681 acccgttgtc tgacacgaac cgtcgcctgg tccagtggta aagtacttgc cggagtctcc
     4741 gtcaccgcat cggccggtaa tgacgtcata atttttgaca tcattttcgg atcatctttt
     4801 gcaatctcag aaggcagcac accgtagatc gcaatccccc acagaccaaa cttaaacggc
     4861 cccttcaggg cagccagcac atcagcaacg gcggccgaca acgcatcgcc ggaaactgat
     4921 aaagccagtc cttcagcccc cggagtagcc agggcaggca gaccgaaggc catcgcagtg
     4981 gccgaagact gtccgccacc agagctaccg ctatgattgc cgctgttacc accattacca
     5041 ccgggtttac tcccgccgtt accaaactcc cccggattat agttatgaat atctccattc
     5101 ggcgtatgcg tcgtacccca gcccggcccg ctgtttggat ccgtaccacc acctgatgaa
     5161 gggccacctt taccagacgt accgttcaca ttcccactgg cctgaccacc attatgtccc
     5221 agacctgaat tacccggacc tcgaccgtct ccaccactca taccgttttc ctctttgaca
     5281 tttaaaacaa taaattaaag ccaacatact gtacatatac acagtataat tattacctta
     5341 ctcctcccgt tttcaggtgt caatatatga gattagttct catcttcagg cacaaaaaag
     5401 cgcccggagc cacctgtaag cagatcgcgt cttttcccga aaatttctca tggcaacaaa
     5461 ttttattatt taagacattc atcacactta catttcagat ggtatagaca catctgaaag
     5521 ctaactggca tgaggagagg acctgcggtt aaactgttcg ggaaacctga gacgcccgga
     5581 ggccactatg acgcacctga aactgactcc cgctgaagaa aagcgcctgg cggacgcctt
     5641 tgtcgctcag gcactggcca tgggcctgaa agggaacaaa acgctgccaa aggcggtaaa
     5701 acaacaggtg cgggaaaggg tgcttcgtgc cattcaggac gaacgtgaag ccaggcgggc
     5761 gcaggaaatg cgtcgggaga aatacgaggc tacggaaacc accttcacct ggcagccaca
     5821 gcgccgccgg taaggggtat ttaccataac gtcgcattgc ccgcaccgct cagacggtct
     5881 gagggcgttt ccggcggcta cgggataaaa caggcaacaa accacccgga aaaccgccgg
     5941 aaacgcgccc ggtaaattgt aacccgctgc atgcctatgc acgcaactgc atggcgggcc
     6001 gtttggtgta aaaacgatac gataagccgt gtttatgtaa atgaacgtgg ccgtaaagag
     6061 tgacaaatat acatgtaaca ttttttactg acaggtcatc aacccgatgc gtttaatacc
     6121 ctttatcctg ctggcattgt gcagcccctt actcctgacg gcctgtaagc attccaccac
     6181 ccagccgccg aaaatttata ctctggataa gtcatctaaa atcagtagct acgctgcggc
     6241 catcaagacc gctgttgaaa tgcagatgtt tgaaccagag gcctatgcgg gaaaaatgtg
     6301 caccgtgcgg atatccatac aacgtgatgg cactctgaac agcgccattg ctgaaggagg
     6361 agatccgaaa ctctgtaaag cagcaatatc agccgttacc cacgccaaaa ttccgccggc
     6421 accggatgaa aatacctggc aaatattcaa aaacgcccct ctggatttca gaccatgaaa
     6481 gcttttactc tgacagtgct ggcggccatg ttcctcactc cggtacatgc gataccacta
     6541 aaacccgtaa ccacactgca gccaacctac ccggtacagg ccgcagcaaa acgcatcgaa
     6601 ggtcaggtga ccgccacgtt tgatattctg gacgatggac gagtggacaa cgtgtcgctt
     6661 accggctgtc accttttttt tcgggaaacg ctgacggcaa tgaggcactg gctatatccc
     6721 gcagggcacc cggcaatgcg acaggttata aattttgaat tcagtctgaa aaatgacggc
     6781 ttacaggatc cgcgaccagt caccacccaa aagctcaatg atgatggttg ccgccagtaa
     6841 caggtccttt aacccgcagg ctgcaatagt cctggatgat gagtgagcct gtagcagccc
     6901 tgattagtgg aagcgaagct gaaaaatggg cagcaaaagc cgtatttctg gcaaatagcg
     6961 ccgccacgga ttaaagtagt catgtgtaat acatatgact acaaaggggc ttttatgtca
     7021 tcagcaacat ccatcaggct cgatgaagaa cttaaggacc ggctgaaaac gctggccgat
     7081 gaccggcacc gctcagcgca cgcgctaatg ctggaggcaa tcaccgaata catcgaccgg
     7141 gaagagaagc gcagccagta cctgcgggac ggacaggccg catggcagca ctatcaggaa
     7201 acgggtctgc accttactgc ggaagaagcg gaagcctgga taagtacctg gggaactgag
     7261 aacgagcagg atgctccgcc atgccacagg taacgatttc cgcgctggca cagcgcgatt
     7321 tacaacgcct tcaggacttt ctcaaaacca aaaatcggct ggcagccaga aaaggaggtg
     7381 aggtgatcgt ccgggctatc cagcaactga aaacattgcc agacattggc cgcccggtgc
     7441 cgtttctgcc gctggaatat aaggaactgg tgatcgggtt tggcgacagt ggctatgtga
     7501 tgctctaccg ccacgaccgg gaaatggacc agattgtgat cgtcaccgtc aggcatcaga
     7561 aagaatccgg gtatccgggg gcagacagcc tctaataacc gatgacgggg gcgcaattca
     7621 ttgcgtggcc gtcgccggtt tgacaagcgc agcgcgtcag aaaaccacat cctgaacgga
     7681 aaaacgggta aaattcagcg atacatttac cgtgagaaat tacacacctt tcacacccga
     7741 taaaatcagg ggtatacagt ggcgaaaaca ttgctcaacc tttcacaggc agcacaagcc
     7801 gcgggcatta ccagaagaac gctatacaat catgtaaaac agggaaaggt taccgtgtca
     7861 cgtgacggga aaaacaaccc tgtcgtggat gtgtcagaac tgatccgtgt atacggaaat
     7921 gtgaatatcc ctgagaaaca aattcccggc atttcacacc gtgaaaatac acaaaaaaat
     7981 ttcccacagg aacagcttct ggcaatgcaa aaggaactgg ccgatctccg gcaggctgtc
     8041 accctgatgc tggaagataa aacatcccgg gaagaagaac gccgccagca cgatgatgag
     8101 cgccggaaac tccaggccga agtggacaga ttgacaacag aactgacaca gaagaaaaaa
     8161 cgcttctggt ccggctggtt tagctgagaa cttaccgaag ggaatggaga acatgacgaa
     8221 gcaggaaaaa actgccctca atatggctaa attcattaag gatcagtcgc tgctgttgct
     8281 ggaaaagctg aacgagctgg acatggatac cccggccgac atgtgcgaac ggcttcacga
     8341 ggatgcggaa acactctggc aggtgatgcg ggataagctc ggggaagaat aacagcaccg
     8401 gccatgccgg tgctggcgtt attttttgcc gaacggccac caccggcggg cgggtttgtc
     8461 ctccggcagt tcaggcaccg gcacgttaaa atagcggctg ttatattccc gcatcagctc
     8521 cgctttcagc ttctcgttca ccgcgctttc cccgtgccgc tcgctgacca cctcaatcat
     8581 cgcgttaatc tgctccggtg agcacttccc ggcatcaacc acctgcacac cgttctcgat
     8641 ggcgaaccgc tccacctttc gatagaggta atcacacagc ttcattcctg tgggtttgtc
     8701 ccgttcccat tgcggatttt caccggtgaa aaagtacgtg gctttttcgt cgctgttgag
     8761 ccgctcgtca tacatcccga tggatttcat catcgccaga tgctgggccc ggtcgacaaa
     8821 acagaaaaca tacgccccgg tctgccaggc gagcaccacc atgtcttcca gatgctcgct
     8881 ggttggcata atgcagcgct cgccgtcttt gtcttccacg tcctcaataa ataccatgta
     8941 cagcgccata atccctcact ccgttgtcat aaatttaaag cacgcgaaca gcctctcccg
     9001 gtccagctcc accgttttgc gggcctcgcc ggtctgtgga ttcacgattt catccaccgc
     9061 cagaatcgcc cgctgtaccc ggacaagcgt cggccggtca gggcagatgt accagatttc
     9121 atcgatgccg ttcgcccggc ggttgaacag atacccggac accacttcct gataccgcct
     9181 gcggctcttg acggtgcgct ctacctcaaa tgccaccgtt ctgccgtcca gctccgccag
     9241 cgcgtccggc actttcatcc ctttcagggc catacggtgc aggtaccgcc agccggtggc
     9301 cccccggccc tccatggaga ggcgcaggga ctgcacggcc agccggtgcg gcagctgcgc
     9361 cgccgacacc cttgacggct gaaacggaat aacgtccgtc agcggctcgt cctcgtcgaa
     9421 cgagaacgcc accccgtgcg gcgtcagccc ccacacgctg acctgacggc cgtcaaccgg
     9481 gtatttcgcc cggaccagca gccctttcgc caccagactg tccaggaagc ggtaaaccgg
     9541 ctgtaccgcc gcaatcccca gccgtcgcgc aaccagctcc gccgtggaac acagctcttc
     9601 ccgcagccag cgcagggtca gccgtgtttt ctccgcgttc cgcgcctgcc gcttcgcgtg
     9661 gctgacgttg tactgctcca gtgtcatcag taaagccccg caaaatcatc atccgctttt
     9721 ggtccgccgc cgtcggccgg aggcaggtac tccgccagcg ggtccgtttt cgccagcacc
     9781 gggaaggttt tcagcgaaat ttcccggcgc tcaacccgca gcgggctgct gaacgccagc
     9841 cgcgccacgc ccagcccggt catcaccgcg aacccgtcag gcaggtgttg cagggtgttc
     9901 acgtcaaaca gcggccgcgc ctccttctgg atgaagctgt cgccgctgat gtgctccacg
     9961 ttccccgcct cccggctggc ccggcggcgc tccacgtcca cccggatctc cccggtctgg
    10021 cccgccgccc actgcgcgga ctcctggctg gccgcgcgat
//
