LOCUS       NZ_CP097394             3770 bp    DNA     circular CON 30-AUG-2023
DEFINITION  Klebsiella pneumoniae strain KPTCM plasmid pKPTCM-9, complete
            sequence.
ACCESSION   NZ_CP097394
VERSION     NZ_CP097394.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28422337
            Assembly: GCF_024637995.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Tian,C., Xing,M. and Wang,S.
  TITLE     OXA-232 and chromosomal CTX-M-15 producing Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Tian,C., Xing,M. and Wang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2022) Department of Pharmacy, Department of
            Pharmacy, Shaoxing Hospital of Traditional Chinese Medicine
            Affiliated to Zhejiang Chi, Renminzhong Road 641, Yuecheng District,
            Shaoxing, Zhejiang 312000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/30/2023 04:11:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,533
            CDSs (total)                      :: 5,409
            Genes (coding)                    :: 5,221
            CDSs (with protein)               :: 5,221
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 188
            CDSs (without protein)            :: 188
            Pseudo Genes (ambiguous residues) :: 0 of 188
            Pseudo Genes (frameshifted)       :: 74 of 188
            Pseudo Genes (incomplete)         :: 119 of 188
            Pseudo Genes (internal stop)      :: 29 of 188
            Pseudo Genes (multiple problems)  :: 31 of 188
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097394.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPTCM"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPTCM-9"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2022-01-12"
     gene            379..528
                     /locus_tag="M6G79_RS27720"
     CDS             379..528
                     /locus_tag="M6G79_RS27720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_162847635.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPGRHDKAPLAAAIDKSRCARDFR
                     ECLK"
                     /name="replication initiation protein"
     gene            complement(796..1794)
                     /locus_tag="M6G79_RS27630"
                     /old_locus_tag="M6G79_27610"
     CDS             complement(796..1794)
                     /locus_tag="M6G79_RS27630"
                     /old_locus_tag="M6G79_27610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="WP_001477600.1"
                     /translation="MGHGVTVLFLLLLLVHIIFDFYLQTEEMVREKAFDNGIGRALKAH
                     LFHAVMHGFFLCLSCFAFIYVLLGCTNLLNFMLFVLLFSLFTFFHFAIDVAKSITSLKV
                     KGFYCFAVFLFDQVLHILTIFFVIYYFFCDYGVYIYDFNFIKYVGGVLIFACSIAFLLK
                     PSSIIVYLFFKSSGIGDGSEKVKITKSKIAELLYIVLNKELGDSDVKDCKVAVNKYKKK
                     AEEIVIGISDGQADIDIRTISKVNNAGRFIGYLERLIIFLFLILGSVTAVVAVLAMKTA
                     LRFSDLKDDNDSGKAEYIMIGTFFSLIVTVIISVLARMYLIEIGIKINDFS"
                     /name="DUF3307 domain-containing protein"
     gene            complement(1805..2473)
                     /locus_tag="M6G79_RS27635"
                     /old_locus_tag="M6G79_27615"
     CDS             complement(1805..2473)
                     /locus_tag="M6G79_RS27635"
                     /old_locus_tag="M6G79_27615"
                     /inference="COORDINATES: protein motif:HMM:NF016205.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059034.1"
                     /translation="MSDSGLMMGVITGDLYRSSTDYERGVSYIGLMEVMLEKIKKNNRL
                     RVSDVDFFRGDSFQITSKDPINLLSIAVYIRAYLRSYSEKNDGKGMYDARLAISIDKVN
                     KYRRDINSIFESAQIKSGKSLDLIENDEMMVFDSSLYDLKEIYSPSIILLDALLSMLSK
                     PQSEILREIVYDRDASISEIAERLGKSKQVVYKTIKRGAVDKIIKFLDAHLSLLGMRFF
                     "
                     /name="hypothetical protein"
     gene            complement(2861..3184)
                     /gene="mobA"
                     /locus_tag="M6G79_RS27640"
                     /old_locus_tag="M6G79_27620"
     CDS             complement(2861..3184)
                     /gene="mobA"
                     /locus_tag="M6G79_RS27640"
                     /old_locus_tag="M6G79_27620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_052522663.1"
                     /translation="MTKRSGSNTRRRAICRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            3468..3662
                     /locus_tag="M6G79_RS27645"
                     /old_locus_tag="M6G79_27625"
     CDS             3468..3662
                     /locus_tag="M6G79_RS27645"
                     /old_locus_tag="M6G79_27625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041619.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENRTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3194..3430)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.49e-121"
     Typing          2..448
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.702"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(2865..3184)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.131"
                     /coverage="100.0"
                     /evalue="1.44e-61"
ORIGIN
        1 ctgcggcgag cggtgtcagc tcactcaaaa gcggtaatac ggttatccac agaatcaggg
       61 gataaagccg gaaagaacat gtgagcaaaa agcaaagcac cggaagaagc cagcgccgca
      121 gacgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      181 gaggtggcga aacccgacag gacttaaaga taccaggcgt ttccccctgg aagctccctc
      241 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      301 ggaagcgtgg cgctttctca tagctcacgc tgttggtatc tcagttcggt gtaggtcgtt
      361 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      421 ggtaactatc gacttgagtc caatccggga agacacgaca aagcgccact ggcagcagcc
      481 attgataaga gtcgatgtgc gagagatttt agagagtgct tgaagtgatg gccggactac
      541 agctacacta aaagaacagt tttggtggct cgtctcgcac aagacagtta cctggttaag
      601 cagttcccca actgacttaa ccttcgaaaa accacctccc caggtggttt tttcgtttcc
      661 agtgcaagag attacgcgca gatataaatg atctcaagaa gatccttctc agatacgaaa
      721 atgcatcatc attccattga tatcatttct catcattgca accatcatct tcttatgtga
      781 gaaactgagc tcaaactaac taaaatcatt aatttttata ccaatctcaa tcaagtacat
      841 tctggctaac acagatatta tcacggttac aataagacta aaaaatgtac caatcattat
      901 atattcagcc ttcccactat cattatcatc ttttagatca ctaaatctca gagctgtttt
      961 cattgctaac accgcgacaa ccgcagtgac cgagcctaaa atcaaaaaca aaaaaattat
     1021 caatctttca aggtaaccta taaatctacc agcattatta actttggaaa tagttctgat
     1081 atcaatatca gcttgcccat cagaaatacc gattacaatt tcctctgctt ttttcttgta
     1141 tttatttact gccaccttgc aatctttaac atcagaatca cccagttctt tattcaatac
     1201 aatataaagc aattccgcaa ttttactttt ggttatttta accttctctg aaccatcacc
     1261 aatacctgat gatttaaaga ataagtaaac tataatagat gaaggcttta gtaaaaacgc
     1321 tatagaacaa gcaaatatca acaccccgcc aacatatttt atgaaattaa aatcatatat
     1381 atatacgcca taatcacaaa agaaataata aataacaaaa aatatagtta aaatatgcaa
     1441 aacttgatca aacaagaaaa cagcaaaaca ataaaaaccc tttactttca gagaggtaat
     1501 cgactttgcg acatctattg caaaatgaaa aaatgtaaat aaagaaaaca gcaaaacaaa
     1561 tagcataaag tttaatagat tggtacagcc aagcagtaca tatatgaaag cgaagcagga
     1621 tagacataga aaaaaaccat gcatcacggc atgaaataag tgagccttta aagccctacc
     1681 aatgccatta tcgaatgcct tttctctaac catctcctca gtttgcaaat aaaagtcaaa
     1741 aattatatga accaaaagca acaacaaaaa tagaacagtt acaccgtgcc ccataccccc
     1801 tccattaaaa aaatctcatt ccaagcagtg aaagatgagc atccaaaaac tttattatct
     1861 tatcaaccgc tcctcttttt atggttttat aaacaacttg tttactcttt cccagtcttt
     1921 ctgctatttc agaaatactt gcatccctat catatacaat ctcccttagg atttctgatt
     1981 gaggtttgga caacattgaa agcaatgcat caagaagaat aatagatggg gaatatattt
     2041 cctttaaatc ataaagtgat gagtcgaaga ccatcatctc atcattttca atgagatcta
     2101 aacttttccc tgactttatc tgagcagatt caaatattga gtttatatcc cttctatact
     2161 tattaacttt atctatggat atcgccaagc gagcatcata catcccttta ccatcgtttt
     2221 tctcactata tgatcttaaa taagccctaa tataaacggc aatacttaat aaattaatag
     2281 gatctttgct ggttatctga aatgaatccc ctcgaaaaaa atcaacatca ctaactctta
     2341 acctattgtt tttctttatc ttttctaaca taacctccat caagccaatg taagaaaccc
     2401 ctcgttcgta atcggtactc gagcggtaca aatcaccagt gataaccccc atcattaacc
     2461 ctgaatcact catttcatcc cccaaactaa aataattctt gacagattgc acaaccgaaa
     2521 aggaaaatag tcaaccaaaa aggttgaaaa acaaaaaagt aacccatttt ggtttacacc
     2581 aataacttga tacttcttgg ctctaaggcg ctcaatatta aaattcagtt gtcaccctgc
     2641 atttaacata aagccataat tgtacgcacc gctgaaacgc gctgaacgcg attctgacgc
     2701 agacaccgca aaaagagatg ttttaccccg ggaaaacagg aaggatgcgt caggatcgtt
     2761 ttcagctcgc tgcatagcta tgcatgaaag cgaatggtga tcactttggg agcttacggt
     2821 gttcataccg tctgttttcg acagttttct ctccgggaag ctaatctgcc ataagcctgg
     2881 ataacagggc acggtgatac tccgtaatag cgatcagcac ctccgcatac tccctgtccc
     2941 cgacacgctt gccgtcgata aagagttttt tctgtaacgc ccccagtctc ataacttctt
     3001 tcagtacccg atcatcagtc agtgagttaa ctttcttacc gagagctgcc gcccgtaaaa
     3061 aaccggaaac ggtcttgccg cattcagcag ccctttttct gatttcctgg tcttcttctg
     3121 ccgttagacg aacagggcga cagatagccc gcctgcgcgt attacttcca cttcgttttg
     3181 tcataaaaca tggctccgta tctgacatgg gtgtcggggc aaagccctga ccagggtaat
     3241 tgtaatagcg tgcatgcatg cgcggtataa caattacaca tcctgtcctg tcagcaagtt
     3301 cgaattaccg acaaagacac ggcagttttt tggcagtgac agtattgtca ggcagaaata
     3361 cccgtcaaag cgtcagacgg cagaataccg gcggtaaaac agtcgaaaag tggtacgcga
     3421 tataatcaca caacgcagat gacagacaga acggagaaaa cgaaaagatg aacaagcagc
     3481 agcaagccgt actcaacatg gcaggattca taaaaagcca gagcctgacg ctgctcgaaa
     3541 aactggacgc actcgatgct gacgaacagg ccgccatgtg tgagaagctg cacgaactcg
     3601 cggaagaact tcagaacagc atacagacac gctttgaagc ggaaaatcgc accgggatat
     3661 aacgcatccg gacaggatgc agaacggcat agaggagcgt aaggagaaaa tgtcacataa
     3721 ggcgctcttc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga
//
