LOCUS       NZ_CP097393             4510 bp    DNA     circular CON 30-AUG-2023
DEFINITION  Klebsiella pneumoniae strain KPTCM plasmid pKPTCM-8, complete
            sequence.
ACCESSION   NZ_CP097393
VERSION     NZ_CP097393.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28422337
            Assembly: GCF_024637995.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Tian,C., Xing,M. and Wang,S.
  TITLE     OXA-232 and chromosomal CTX-M-15 producing Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Tian,C., Xing,M. and Wang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2022) Department of Pharmacy, Department of
            Pharmacy, Shaoxing Hospital of Traditional Chinese Medicine
            Affiliated to Zhejiang Chi, Renminzhong Road 641, Yuecheng District,
            Shaoxing, Zhejiang 312000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/30/2023 04:11:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,533
            CDSs (total)                      :: 5,409
            Genes (coding)                    :: 5,221
            CDSs (with protein)               :: 5,221
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 188
            CDSs (without protein)            :: 188
            Pseudo Genes (ambiguous residues) :: 0 of 188
            Pseudo Genes (frameshifted)       :: 74 of 188
            Pseudo Genes (incomplete)         :: 119 of 188
            Pseudo Genes (internal stop)      :: 29 of 188
            Pseudo Genes (multiple problems)  :: 31 of 188
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097393.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPTCM"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPTCM-8"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2022-01-12"
     gene            424..870
                     /locus_tag="M6G79_RS27600"
                     /old_locus_tag="M6G79_27580"
     CDS             424..870
                     /locus_tag="M6G79_RS27600"
                     /old_locus_tag="M6G79_27580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125450400.1"
                     /translation="MNDKSGFSFMKIGAGAKVTGLRMKRNVMVVDDLSHVESFVTVGER
                     GEVINLDADGNELHTPDSYREKLKIERGELQASLEKAKEEINSIDNDITSQKIKNQLSA
                     ALSQPMDAEGEWNTKRILQGVLDLSKQVGCGVLTAAITHYLGVS"
                     /name="hypothetical protein"
     gene            complement(1412..1654)
                     /locus_tag="M6G79_RS27605"
                     /old_locus_tag="M6G79_27585"
     CDS             complement(1412..1654)
                     /locus_tag="M6G79_RS27605"
                     /old_locus_tag="M6G79_27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_071993219.1"
                     /translation="MALVRSGNKTKIFEADPKFNPVFDTQHKPGDTCTYEGIYKCTGCG
                     KEVTIAGGKTLPPQNHHQHSPGQGDIRWQLFVYAE"
                     /name="protein L"
     gene            1837..2157
                     /locus_tag="M6G79_RS27610"
                     /old_locus_tag="M6G79_27590"
     CDS             1837..2157
                     /locus_tag="M6G79_RS27610"
                     /old_locus_tag="M6G79_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_072198282.1"
                     /translation="MQQNHDSDDDCRIQFRDDWLYQFYKYGKANRKIPATISVSLGRKL
                     DMLNAADKIGDLRSPPGNRFENLNPPLTGYSSIRVNDKYRLIFKWGENGVEDLYLDPHD
                     YR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2361..2717
                     /locus_tag="M6G79_RS27615"
                     /old_locus_tag="M6G79_27595"
     CDS             2361..2717
                     /locus_tag="M6G79_RS27615"
                     /old_locus_tag="M6G79_27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_032413514.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIERVTPAHEIIER
                     KHNAESLEKKHAVA"
                     /name="HigA family addiction module antitoxin"
     gene            3546..3737
                     /locus_tag="M6G79_RS27620"
                     /old_locus_tag="M6G79_27600"
     CDS             3546..3737
                     /locus_tag="M6G79_RS27620"
                     /old_locus_tag="M6G79_27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032421723.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3737..4147
                     /locus_tag="M6G79_RS27625"
                     /old_locus_tag="M6G79_27605"
     CDS             3737..4147
                     /locus_tag="M6G79_RS27625"
                     /old_locus_tag="M6G79_27605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060578860.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKIIKVID
                     TSELIRVFGSLDGVIDTVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(3018..3427)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcgccggtga atcgtgtggt gagtcgtccg gcgacttgca taacgtgatt gccggccgtc
       61 gacgaacccg attcctgaac gtgctgaaaa ccgtttttcc ggctgcgccg gcaacgcttc
      121 acggccacgc aataaattgc gtccccgttc accgttttca gtctggtttg gtgcgggttt
      181 tgagttgtct ggcctgaatt caggctgaaa tccgccacca gtcagtctgg tgcataacaa
      241 ttcatgcccg taaaacgccc tggagcgatt ttgaggcgtt ttcagggggt aagacgtagg
      301 atttaatgga ttacgcccca gaatcgtttc tagagcgttt gagcggtgcg cgtaatgagg
      361 cgttatggta aataattgct cgtgaaaacg ttattaacat ttttatctta tgaggtgatg
      421 tgagtgaacg ataaatcagg atttagtttt atgaagatcg gtgcaggtgc aaaagtgact
      481 ggtttaagaa tgaaaaggaa tgtgatggtt gttgatgatc tgtctcacgt agagagcttt
      541 gttactgttg gtgagcgagg cgaagttatt aatctcgatg cggatgggaa cgaactacac
      601 acaccagaca gttatcgtga aaagttaaaa atagaacgcg gtgaactcca ggcttccttg
      661 gagaaagcca aggaggaaat caactcaata gataacgata taacttcgca aaagattaag
      721 aaccagttga gtgctgcact atctcaacca atggatgcag aaggtgagtg gaacactaag
      781 agaatcctac aaggggtttt agatttatct aaacaagtgg gctgtggtgt actcacggct
      841 gcgatcacgc attatcttgg agttagttaa taaactacct atgcccccat ggtgggtata
      901 caaaaaccat ggggagcgga gcgaccttcg tctagctctt ttcttgtgtg tctcatcttg
      961 caacggattc gatttgtgtg gggttagtgt cagacttctt ggggtcagtt tggatataga
     1021 gaattattgt acggtaagcc tgtgttttga acaggcttac ctctcgcaat gatgtactcc
     1081 cgcgctatac gccgtttccc gtgaattttc cttaatcgga taacctaaat gcgatacaga
     1141 aagagcggtg acttaaaagc gatcgtagct gaggtaaaat caatccctta cgcatcaata
     1201 ttggttaaat aatgcgctta acgtacaaaa gtttccgata tccaaactga cccctatttg
     1261 gcggcttttc ttattttctt gattttcccg catctgaacg tttctctcgg actcttccat
     1321 tattattacg atctcgtggc tgagttccgc ctcctggttt agggacgacg acatgcgggt
     1381 taagtggtct acggtctgcc attttcatac ctcactctgc ataaacaaat agttgccagc
     1441 ggatgtcccc ctgtccggga ctatgttgat gatgattttg tggtggaagt gttttccctc
     1501 ctgcgatagt tacttcttta ccgcaacctg tgcatttgta aattccttca tacgtgcacg
     1561 tatcgccagg cttgtgttgt gtatcaaaaa ctgggttaaa tttcggatct gcttcaaaaa
     1621 tcttggtttt atttcctgag cgaacaagcg ccatatgacc tccaactatt ctttttttgc
     1681 acagtagcac tgtgcttttg tgcattttat atcattatgg ttttttgcac aagtgcacag
     1741 tgctattgtg catttctggc gtttgttgta ctatggtggt ggtaaaaggt gatctagaac
     1801 ttgccaagtt gcggcaacat ttgaggtaaa agaaaaatgc agcaaaatca tgatagtgat
     1861 gatgattgcc gcattcagtt ccgagacgat tggctatacc agttttataa atacggtaaa
     1921 gccaatcgaa aaattccagc tacgattagt gtttcattag gtcgcaagct ggatatgctg
     1981 aatgctgctg ataaaattgg agatttacgg tctcccccgg ggaaccgatt tgaaaatctc
     2041 aaccctccat tgacagggta ttcatctata agggtgaatg ataagtaccg cttgatattt
     2101 aagtggggtg agaacggagt cgaggatttg tatctcgatc cacatgatta ccgataggta
     2161 acagaccgga actgttatct tacaaaacaa gtaggtgagg catttcctca cctacgttaa
     2221 aacgtcatca caaataatga ttaataaagt aacgcgacgt aactatattt aagataatta
     2281 ctaatcgata cagctggtaa cagaagaaat acttatcttc ctcatctaac agagagaagc
     2341 gaccaattca ggagtaaagc atgtcccaag caaccagaaa accatcgact gtaggagata
     2401 ttattcttta tgaatatctc gagccaagtg gtttaaaaat caatgacttg gctgaaatgt
     2461 taaacgtgca tagaaattct gcaagtgctt tggttaacaa taataggaaa ctgtctaccg
     2521 atatggcgtt tcggttagct aaagcattcg atactaccgc taagttctgg ttaaatatac
     2581 agcagtctgt tgatttgtgg gaggttacga gcagcccacg ccttcaggaa gaaatagaaa
     2641 gagtcactcc cgctcatgaa attatagaaa ggaagcacaa tgctgaatct ttggaaaaaa
     2701 aacatgcggt cgcatgattt ttttaaaaaa atttaaccaa actagaagaa ggcctctgta
     2761 atagtgtgcc ttcttttttt gtaaccgcta aaggtcatca tcattttctt ttacgagcac
     2821 ttgagatccg gtttcggtac gcgtaatctc ttgctctgta aacggaaaaa ccgccttgca
     2881 gggcggtttt tccgaaggtt caaacacttg caggtgattg aaccaaggta actggcttgg
     2941 aggagcgcag tcaccaaaac ttgttctttc agtttagcct taacaggcgc atgacttcaa
     3001 gactaactcc tctaaatcag ttaccagtgg ctgctgccag tggcgctttt gcatgtctta
     3061 ccgggttgga ctcaagacga tagttaccgg ataagacgca gcagtcggac tgaacggggg
     3121 gttcgtgcat acagtccagc ttggagcgaa ctgccttccc ggaactgagt gtctggcgtg
     3181 gaatgagata aacgcggcca taacagcgga atgacaccgg taaaccgaat ggcaggaaca
     3241 ggagagcgca cgagggagcc atcaggggga aacgcctgat atctttatag tcctgtcggg
     3301 tttcgccacc actgatttga gcgtcaattt ctatgatgct tgtcaggggg gcggagccta
     3361 tggaaaaacg gccgctaagc ggcctttcct tctgcccatc acgcgtccgc tcggttttct
     3421 cgagcattta tacgcatagt cctaccgctc gccgcagccg aacggcagga gcgtagcgac
     3481 tgagtgagcg aggaagcgga aaagagactg gttttacact gagcactgac gctctgaggc
     3541 ttcttatgac gaaacaacag aaaacagcgc tgaacatggc taaattcatc cagaatcagt
     3601 ctctgctcct gctggagaag cttaacgagc ttgatcttga tgcagaggcc gacctttgtg
     3661 agaaactcca cgatgatgcg gagcatcttt tccgtacact gtcctcgaga ctggatgaat
     3721 tacaggatgg taactgatgg ccagagtgtc tattagtgaa gcggcacggc ttgttaaggt
     3781 tagtcgcccc acaatttaca agatgattaa cagtgggaag ttaagttaca cctctgttgt
     3841 aaagcacggg aagatcatta aagttattga tacttcagag cttattagag tttttggttc
     3901 tcttgatggt gtcatcgata ctgtaaaaag tgacgcagga tctacaggtg ttaacagcgt
     3961 tggtttacat gatttacaac atagaattgc tttactagaa gctgaaaacg atggtcttaa
     4021 aggggctgtt aaggctcgag atgagcatat tgattccctt cgtcaggcta tgcagcttct
     4081 ggagcacaag cacgaacctt cttcaccacc cgattccccg tggtggaaat tctggaaaaa
     4141 atcctgactc cgtcatgctt ccgttttcct ctccggccat cccggggagt tcctgaacct
     4201 tcccaaaaat cgccagatct ctgaacgctc ctcaccacta aaaataccca caccccacgc
     4261 aaaattaaaa ttttgaagat aattcattaa tatcagttag ttgtgggtta ttacattacg
     4321 cagtattact ttctgtaagg tgaatccaag tcgtcggcga ttcgtgtacg acttgccgac
     4381 gactcgtcat agagtcatcg acgactcact taagactatc agtaacttat gacgacttga
     4441 ctctagagtc gaatcgtcat tttggtgttt ttttgacctt ggcgactcta gagtcaagtc
     4501 acgagtcgaa
//
