LOCUS       NZ_CP097392             5640 bp    DNA     circular CON 30-AUG-2023
DEFINITION  Klebsiella pneumoniae strain KPTCM plasmid pKPTCM-7, complete
            sequence.
ACCESSION   NZ_CP097392
VERSION     NZ_CP097392.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28422337
            Assembly: GCF_024637995.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Tian,C., Xing,M. and Wang,S.
  TITLE     OXA-232 and chromosomal CTX-M-15 producing Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Tian,C., Xing,M. and Wang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2022) Department of Pharmacy, Department of
            Pharmacy, Shaoxing Hospital of Traditional Chinese Medicine
            Affiliated to Zhejiang Chi, Renminzhong Road 641, Yuecheng District,
            Shaoxing, Zhejiang 312000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/30/2023 04:11:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,533
            CDSs (total)                      :: 5,409
            Genes (coding)                    :: 5,221
            CDSs (with protein)               :: 5,221
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 188
            CDSs (without protein)            :: 188
            Pseudo Genes (ambiguous residues) :: 0 of 188
            Pseudo Genes (frameshifted)       :: 74 of 188
            Pseudo Genes (incomplete)         :: 119 of 188
            Pseudo Genes (internal stop)      :: 29 of 188
            Pseudo Genes (multiple problems)  :: 31 of 188
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097392.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPTCM"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPTCM-7"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2022-01-12"
     gene            complement(189..1964)
                     /locus_tag="M6G79_RS27560"
                     /old_locus_tag="M6G79_27540"
     CDS             complement(189..1964)
                     /locus_tag="M6G79_RS27560"
                     /old_locus_tag="M6G79_27540"
                     /inference="COORDINATES: protein motif:HMM:NF015210.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="WP_124046799.1"
                     /translation="MRFDASPFNMNSILSVEKRYEIPRNQREFSWERIQLEELWADLLR
                     NIVMDDNGTIKLHEYFIGTIVLSGVDSDDVLEVVDGQQRLSVITMMLSIISRYFRKNKN
                     DTTADNIYKKYIVTINNTLDRNSLNSDGESVIQKLTRSVGGEYFKKIVQGKEESDAKAT
                     NDEEKKIRYAVTFFKRALRKKSLCSAIFKTNDDSFTKADYDLCLNAIYKMITNYLYIVR
                     IAVDRGDDAYDIFEVLNARGINLSSIDLIKNKVFQSCTTTFPEDEARRKWVHIYEQLGS
                     VGETMTDYIRCWWLSKYGYIGEDQLYRSFKREILNENSLLTADSFLEEIYNDVSLYMSM
                     VAPSTKYWNLSHQRPILDALANINTFSVTVPRPFILSLLRTRRDKSRLMKQSELIETLE
                     FLEKFHFKFNAVCRMRPSGIDSKYSVFAVRADRAVSKASLKNTIDETVDFLKRKAPSEK
                     VFKDCLMKNIEYKDDKLSQRKLIIYIFETLERLAVGTKELKYHMVSIEHIGSQSNFSPT
                     YVGKLGNLLPLCFDINRDCENMPLAGKLPEYARSRLELVKQFNTANAAKNTWEVSDAQT
                     RQEQITNALNAAFTL"
                     /name="DUF262 domain-containing protein"
     gene            complement(1954..2847)
                     /locus_tag="M6G79_RS27565"
                     /old_locus_tag="M6G79_27545"
     CDS             complement(1954..2847)
                     /locus_tag="M6G79_RS27565"
                     /old_locus_tag="M6G79_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_044816547.1"
                     /translation="MNNAVLELFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRTGAAIDWNDRNAPAPTLTITNPDNGHAHLLYALRTSIRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHSHWRIAVWQPELYTLDWLADFLDLNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYDQWLQACYERATAYNLHFSSPLD
                     DNEVTGIAKSIAKWTSKNFSEDSFREFVSRTHTSEIQSIRGSKSKGGGRPSIGEPWLEH
                     GISRATYFRKLKKNEI"
                     /name="replication initiation protein"
     gene            complement(3040..3324)
                     /gene="nadS"
                     /locus_tag="M6G79_RS27570"
                     /old_locus_tag="M6G79_27550"
     CDS             complement(3040..3324)
                     /gene="nadS"
                     /locus_tag="M6G79_RS27570"
                     /old_locus_tag="M6G79_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_001697687.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="nadS"
     gene            complement(3326..3658)
                     /locus_tag="M6G79_RS27575"
                     /old_locus_tag="M6G79_27555"
     CDS             complement(3326..3658)
                     /locus_tag="M6G79_RS27575"
                     /old_locus_tag="M6G79_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_044816545.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAVLKSLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3814..4059)
                     /locus_tag="M6G79_RS27580"
                     /old_locus_tag="M6G79_27560"
     CDS             complement(3814..4059)
                     /locus_tag="M6G79_RS27580"
                     /old_locus_tag="M6G79_27560"
                     /inference="COORDINATES: protein motif:HMM:NF016769.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_306589344.1"
                     /translation="MTKTMTEMEKQLLSALESLQTSYEQQQQAWQDSYSSLQHMFEATS
                     QALTHSDRVCQHLSSQVDSLRVQVESLSRNVSRLMR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4108..4593)
                     /locus_tag="M6G79_RS27585"
                     /old_locus_tag="M6G79_27565"
     CDS             complement(4108..4593)
                     /locus_tag="M6G79_RS27585"
                     /old_locus_tag="M6G79_27565"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_139914218.1"
                     /translation="MSEILNLAKAFEEKSKQQAQDTEQSVKREFARLETSISAELQSSS
                     QSISNAIREQNGRLTGMLRASVSTAMAWIFLCFALLIGIMGGIIWFQGTIITSRLSEMD
                     SYSQQLESLKAKSGAGVAIYSDDKHRNTYMVVLPKQAHGVETYTSTTDNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     gene            complement(<4640..5062)
                     /locus_tag="M6G79_RS27590"
                     /old_locus_tag="M6G79_27570"
                     /pseudo=""
     CDS             complement(<4640..5062)
                     /locus_tag="M6G79_RS27590"
                     /old_locus_tag="M6G79_27570"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5271..5597)
                     /locus_tag="M6G79_RS27595"
                     /old_locus_tag="M6G79_27575"
     CDS             complement(5271..5597)
                     /locus_tag="M6G79_RS27595"
                     /old_locus_tag="M6G79_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_038885858.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHTSRK
                     RRVPEVAPELLRQVSGIGNNLNQIARRLNQADSLTPSERASLLVVLTSLDRQLGDLLEQ
                     NRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(3723..5281)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.743"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcagtggatg ctgggggttt cggggggcgg cgagccccct gaaccagtca cggcgacacc
       61 tcgtaagagg ggacgctgtg tgtactggct tatagtatga agtagtatgt cagattctat
      121 gctatccgca aaaaaacagc cctctgctaa agagggctgt tatctgatat aggttgttta
      181 gtctcacatc agagcgtgaa ggcagcgttg agggcgtttg ttatctgttc ttgcctcgtt
      241 tgggcatcac ttacttccca agtattttta gctgcgttag cagtgttaaa ttgcttcact
      301 aactctaatc gtgatcgagc atattctggc aatttcccag ctaatggcat attttcacag
      361 tctctattta tatcgaaaca aagcggcaga aggttaccca gcttaccaac ataagttgga
      421 ctgaaattcg actgagagcc tatatgttca atcgaaacca tatgatattt aagctcttta
      481 gttcctactg ctagtcgctc tagtgtttca aatatgtaga taatgagttt tctttgagat
      541 agtttatcgt ccttatactc gatatttttc attaagcagt ctttaaacac cttttcgctt
      601 ggtgcctttc ttttcaggaa atcaactgtc tcatcgattg tgtttttcaa tgaagccttg
      661 ctcacagctc tatcagctct gactgcaaaa actgaatatt ttgagtcaat acctgaaggt
      721 ctcattcgac atacagcatt aaatttaaaa tggaacttct caaggaactc tagcgtctca
      781 ataagctcag actgtttcat aagccttgat ttatctcttc ttgttctgag aagagataaa
      841 atgaaaggtc ttggaacagt aacgctaaat gtgttgatat ttgctaaggc gtctaatata
      901 ggtctctggt gagacaggtt ccagtatttt gtagatggag ctaccatact catataaaga
      961 gatacatcat tatatatttc ttctaaaaat gaatcagctg ttaacaagct attttcattt
     1021 aaaatttctc ttttgaatga tcgatagagc tgatcttcac caatatatcc atatttagat
     1081 agccaccaac aacggatgta atcggtcatg gtctcgccaa ctgaacctag ctgttcgtat
     1141 atgtgaaccc atttccttct ggcttcatct tctgggaaag ttgtagtgca agactggaaa
     1201 accttattct ttataaggtc tattgaactg aggttaatac ctctggcatt tagaacttca
     1261 aatatgtcat atgcatcgtc gcctcgatca accgctatcc tgactatgta cagatagttg
     1321 gttatcattt tatagatagc attaagacat aagtcataat cagccttcgt aaaactatca
     1381 tcatttgttt taaatatggc gctacataat gattttttgc gaagagccct tttaaagaac
     1441 gtaacagcat atcttatttt cttttcttca tcattcgttg ctttggcgtc agactcttcc
     1501 ttgccttgaa ctattttttt aaaatactca cctccaacgc tccttgttag cttttgaata
     1561 acagactcac catcagaatt taaactattt ctatccagag tgttatttat tgttacgata
     1621 tattttttat atatattgtc cgcagttgtg tcatttttgt tctttctaaa ataccttgat
     1681 attatagaaa gcatcatggt tattacagac aatcgttgct gtccatccac aacttctaaa
     1741 acatcatcac tatcaacacc tgatagcaca atggttccaa taaagtactc gtgtaatttt
     1801 atagtgccgt tatcatccat cactatattt cttagaagat cagcccaaag ctcctcaagc
     1861 tgaattcgct cccagctaaa ctctcgctga tttcttggga tctcatatct tttttctaca
     1921 gaaagaatgg agttcatatt aaatggtgac gcatcaaatc tcattttttt ttagtttcct
     1981 gaagtatgta gctctgctga tgccatgttc taaccacggc tcccctatag aaggtcttcc
     2041 gcctccctta cttttactac cccgtattga ctggatctca gaggtatgtg tccgagaaac
     2101 aaactccctg aatgaatctt cggaaaaatt tttacttgtc catttagcta tgcttttggc
     2161 aattccagtt acttcgttat cgtcaagagg cgaagaaaaa tgaaggttat aggctgtagc
     2221 acgttcataa caggcttgca gccactggtc atactcaggc cagccctgac ggatagcgcg
     2281 gtatgcccac ttgcgggttt tgtcgaacag cgtgcagtta cggcctaaac catagtcggc
     2341 aacgatttct ttgtcgttcg ccgcgttcag gtcgagaaag tcagccagcc agtccagcgt
     2401 gtagagttcc ggctgccata cggcaattct ccagtggcta tggttcggat ttttgcagat
     2461 tagccccgaa taccccgcgt cagcctctaa ctttttacgt agcgcgttct ccacagcggc
     2521 agcgtagcgc agcggcttca tttttccgtc cggcgcggta cgtatcgagg ttctgagggc
     2581 gtacagcaga tgcgcgtgtc cgttgtcggg gttggtgatg gtcagcgtgg gcgcaggcgc
     2641 gttccggtcg ttccagtcga tggccgcacc ggtgcgatcg acgtcgaatc ccagccagaa
     2701 catggcgtgc ggctggttga actggatgta tttcgccaga atggcgcgtt ccttaccggc
     2761 aatgcgtaca ccaaagtgta aatcatccga gaagtacggc ttgtgcggta agcggtcgtt
     2821 gaaaagttcg agtactgcgt tattcaaagc gccctgcact tatggcgggc tgttgtctgg
     2881 cacggttcat gtgctaatat cctttcaagg tttcgacctg gccctggtta tcatgtccgc
     2941 tcgccaaagt agagcatgat ttccggggct ttgttttttc tgttgccgtt aaattacacg
     3001 cgaacggcct cgcctgtcga atccttctgt cacaacggct tagtgcgccg ccagctcctg
     3061 gaagaatttc gggttcgcct ggatggttgc cagcaccttc gccgccagtc cggtcggatt
     3121 acggcgtccg gtttcccagc tcttgatagt gtcgaggctg gtccccagca ctttcgccat
     3181 ctcgctctga gatacgttca gctgtgccct gatggctttc acgtctgcca gctcgtaacg
     3241 ggtgacgcgg gcaggctctc tgacgccgtt tttgatttca acggcttctt ccagcgatgc
     3301 tttcagctcg tcaaaaaaac tcatgtcaca cctcgtcttt cagttgttgg gtcagcgact
     3361 tcagcacggc tttctccgcg tccgtcaggc tgtctttggt gcttttcggg taggccatca
     3421 ccagataaat cacctcggcg gtggccagaa agtaaataac cctcgcactg ccgctttttc
     3481 cctggttgcc cgtcgccatt ctgattttgc gcagcccacc ggttttctgg atgagatcgc
     3541 ctttatccgg tgactcaatc agcgtctttt gcagctccct gagttcttca tccgtggcga
     3601 tctgttttat ctgacgggta aacatcgggg tctcgataaa ctcaatcgcg tgactcacgg
     3661 ctgtctcctg tgttcaatgg cgtacatagt acacttaatt ttaatttcag aaaagcggtc
     3721 acatcgaggg gccgtaatag cgggtcggct ttttctgcgg ctccggctgc tgctcgcgca
     3781 gcttctccgc acgggcgcgg tcgtgctgtg caatcagcgc atcaagcggc tgacgttcct
     3841 gcttaagctc tcgacctgca cgcgcaagct gtccacctga ctgctcaagt gctgacacac
     3901 cctgtcgctg tgcgtcagcg cctgtgaggt agcctcgaac atgtgctgca agctgctgta
     3961 gctgtcctgc cacgcctgct gctgttgctc gtagcttgtc tgtaagctct ctaaggcgct
     4021 cagaagctgt ttttccatct cggtcatggt tttggtcatc ctcggtgtta aaaacgcgct
     4081 gtgtcgtttc agggcgctct gacggggtta tttcgccgtg tatttcacta cggtgttgtc
     4141 ggtcgtcgat gtataggttt ccacgccgtg cgcctgcttc ggcagcacca ccatgtaggt
     4201 gtttcggtgc ttgtcgtcgc tgtagatcgc cacgcccgcg ccgcttttcg ctttcaggct
     4261 ctccagctgt tggctgtagc tgtccatctc gctcaggcgc gaggtgatga tcgtgccctg
     4321 aaaccagatt atcccgccca tgatgccgat cagcagggcg aagcagagga atatccacgc
     4381 catcgccgtt gagacagagg ctctgagcat gcccgtcagt ctgccgttct gctcgcggat
     4441 agcgttgctg atgctctgcg agcttgattg cagttccgcg ctgatagacg tctccagcct
     4501 cgcgaactcg cgcttcacgc tctgctcggt atcctgcgcc tgctgcttcg atttctcctc
     4561 gaaggccttc gccaaattta aaatctcgct cataaatcat tcctttcagc cggatatttc
     4621 ggccgccgtc cggatcggca atgctgatgc tgcttttcgt ttcccgcgct acggtcaggc
     4681 cgtagctttc cagctgcgtc accacgtcct gccggctgcg gatcaccccc tgctgcatca
     4741 ggttcatcag gccggcggtg attttctctg ccgcctgctg tttgtcgcgg ggcaaatcgc
     4801 tggcctgcgt cagcggacgg cggtaggtcg ggtcattcgg gtcgcgcagc cccagccggt
     4861 cgttggtcag ggtctgccag gcgttgacgc ggggacggtc ggcgcggtca aagtagggtt
     4921 gcaggcgttt tccactctgc aattcgatgt tcgggatgac aaaattcagc tcaagccgtc
     4981 ccttgtcacg gtgctccacc cacaggcagg cgtactggtc gcgatcaagg ccggtcagca
     5041 gcgtgtgctc ccattcgtcc atcaggcgcg atttctcagc gtctgcaatg tccggctcct
     5101 gaaatgacag cacgccggaa gtataagcgc gggcaaagtc gcagccgtcg atcagctcgc
     5161 gcacatgttc agggttgccg cgcagaacgc tggcctgttc acgctgccgg tctttaccca
     5221 gcaggtaatc caccggcccg ctgcccgaac ccgcaccccg tgagtgaatt ttaacgatca
     5281 cggttctgct ccagcaggtc gccaagctgc cggtcaaggc tggtcagcac gacgagcagc
     5341 gaggcacgtt cagaaggcgt cagggaatca gcctggttca ggcgacgggc gatctggttc
     5401 aagttattgc caatcccgct gacctgccgc agcagttcgg gcgccacctc aggcacacgg
     5461 cgcttgcggg aggtgtgttc acccagacca agctgacgca gccactccgc aaggtgagtg
     5521 cggtcgcagc gatcaagcag tcgctgatgc tcggtttcgg tgagccggat tttgatctct
     5581 ttcgttcgct tttccatagg ctccgctgag aaaaaattcg caccaccgtg agaatttttc
//
