LOCUS       NZ_CP097390             9730 bp    DNA     circular CON 30-AUG-2023
DEFINITION  Klebsiella pneumoniae strain KPTCM plasmid pKPTCM-5, complete
            sequence.
ACCESSION   NZ_CP097390
VERSION     NZ_CP097390.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28422337
            Assembly: GCF_024637995.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Tian,C., Xing,M. and Wang,S.
  TITLE     OXA-232 and chromosomal CTX-M-15 producing Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Tian,C., Xing,M. and Wang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2022) Department of Pharmacy, Department of
            Pharmacy, Shaoxing Hospital of Traditional Chinese Medicine
            Affiliated to Zhejiang Chi, Renminzhong Road 641, Yuecheng District,
            Shaoxing, Zhejiang 312000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/30/2023 04:11:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,533
            CDSs (total)                      :: 5,409
            Genes (coding)                    :: 5,221
            CDSs (with protein)               :: 5,221
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 188
            CDSs (without protein)            :: 188
            Pseudo Genes (ambiguous residues) :: 0 of 188
            Pseudo Genes (frameshifted)       :: 74 of 188
            Pseudo Genes (incomplete)         :: 119 of 188
            Pseudo Genes (internal stop)      :: 29 of 188
            Pseudo Genes (multiple problems)  :: 31 of 188
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097390.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPTCM"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPTCM-5"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2022-01-12"
     gene            1343..3304
                     /locus_tag="M6G79_RS27480"
                     /old_locus_tag="M6G79_27460"
     CDS             1343..3304
                     /locus_tag="M6G79_RS27480"
                     /old_locus_tag="M6G79_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3304..4035
                     /gene="mobC"
                     /locus_tag="M6G79_RS27485"
                     /old_locus_tag="M6G79_27465"
     CDS             3304..4035
                     /gene="mobC"
                     /locus_tag="M6G79_RS27485"
                     /old_locus_tag="M6G79_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            4042..4572
                     /locus_tag="M6G79_RS27490"
                     /old_locus_tag="M6G79_27470"
     CDS             4042..4572
                     /locus_tag="M6G79_RS27490"
                     /old_locus_tag="M6G79_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4600..4779)
                     /locus_tag="M6G79_RS27495"
                     /old_locus_tag="M6G79_27475"
     CDS             complement(4600..4779)
                     /locus_tag="M6G79_RS27495"
                     /old_locus_tag="M6G79_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4848..5225
                     /locus_tag="M6G79_RS27500"
                     /old_locus_tag="M6G79_27480"
     CDS             4848..5225
                     /locus_tag="M6G79_RS27500"
                     /old_locus_tag="M6G79_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(5287..5586)
                     /locus_tag="M6G79_RS27505"
                     /old_locus_tag="M6G79_27485"
     CDS             complement(5287..5586)
                     /locus_tag="M6G79_RS27505"
                     /old_locus_tag="M6G79_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5576..5839)
                     /locus_tag="M6G79_RS27510"
                     /old_locus_tag="M6G79_27490"
     CDS             complement(5576..5839)
                     /locus_tag="M6G79_RS27510"
                     /old_locus_tag="M6G79_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            6216..7301
                     /locus_tag="M6G79_RS27515"
                     /old_locus_tag="M6G79_27495"
     CDS             6216..7301
                     /locus_tag="M6G79_RS27515"
                     /old_locus_tag="M6G79_27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            7298..9487
                     /locus_tag="M6G79_RS27520"
                     /old_locus_tag="M6G79_27500"
     CDS             7298..9487
                     /locus_tag="M6G79_RS27520"
                     /old_locus_tag="M6G79_27500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     Typing          complement(2..543)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.527"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3305..4032
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 cagagcacag caaccaaatc tgtccttcca gtgtagccgg actttggcgc acacttcaag
       61 agcaaccgcg tgtttagcta aacaaatcct ctgcgaactc ccagttacca atggctgctg
      121 ccagtggcgt ttttgcgtgt ctttccgggt tggactcaag tgaacagtta ccggataagg
      181 cgcagcagtc gggctgaacg gggagttctt gcttacagcc cagcttggag cgaacgacct
      241 acaccgagcc gagataccag tgtgtgagct atgagaaagc gccacacttc ccgtaaggga
      301 gaaaggcgga acaggtatcc ggtaaacggc agggtcggaa caggagagcg caagagggag
      361 cgacccgccg gaaacggtgg ggatctttaa gtcctgtcgg gtttcgcccg tactgtcaga
      421 ttcatggttg agcctcacgg ctcccacaga tgcaccgaaa aagcgtctgt ttatgtgaat
      481 tctggcagga gggcgaagcc tatggaaaaa cgccaccggc gcggccctgc cgttttgcct
      541 cacatgttag tcccctgctt atccacggaa tctgtgggta actttgcgag agttagcagc
      601 gcccgccgca gtctcacggc cggagcgtag cgactgagtg agggaggagg cgcaatatcc
      661 cccctcccgc agacggccac cggacgcgcg gatgtgacat attgcaccgt gttaccgcct
      721 ttcccttccc tgccgataaa tcattcagat aggtgaagtc tgtcggtttt ctgctgaata
      781 ttttacgtta acctatctct gttgcttttt tctccttgcg gagaaatgca ggtcatcccc
      841 gctggggcgt ggctgacttc aggaggaaat cccctgctta cgctggctgg acgccaccga
      901 cgcacggtct ttccccctct ttctggctca gctcggctgg acgccggcca cgccagaaat
      961 tcacccggcc cccgtcaggc tcacgcctga ccacccacaa aaacccacgg tgctgaacgc
     1021 acctgacgaa gcagcaaagc tgctcctgat tttgcgggtg gtcagccgct cgctatggcg
     1081 aactctgacg ggggccggaa caccggaaaa gaccgcactg gggtgccgcc ttttcctctc
     1141 acacgctggg gcgtgctcgt tgttcctccg gggctgcgcc ttttccgctt ggggcggaaa
     1201 gaggctggcc tccgtggtga aagctgaatg caatcaccac ggacgtgacc gccgccgcca
     1261 tgcctcgggg catgtccgcg gcttgtgcag caggggctgc acaccacgcc ggtaaacccg
     1321 tcgtctgaag gagagagatt gcatgtttaa tacggattcg cttgcctggc agtggcatat
     1381 cccggagctg gcccggcaga tttacccgtg ggtcagctgg ctgtacaggg agtattacgg
     1441 tctggccggg gctttcctgg ttatcggcct gtttttcggt ctggctgaac tctgggccgg
     1501 acggatgcgt tttgtggggc tgagtcataa ggagcgacgg gagtatgccg atcgctataa
     1561 gcgcgataat aaccggactg cgccattttc ggtgctggct tcgctggatt cgcccgggcg
     1621 gatggtcagg gcacggcggc atgtctggaa ctacgcgtac tggctgaccg gcgctgccgg
     1681 tctggccctt ctgggggggc tggttgtcgc cgtgtttatc gagagctggg gagaaaccgt
     1741 cctgcgtccg gacctctacg cgaacgcaga ttattacctg ctgcagccgg ctaacctgtt
     1801 cgggattgtc tggtgtgccg gagggcttgt cgccggcatc cttctggcgc ggctgctggc
     1861 atttctgctg cttgacggtc attttgcggc cgctgacgag gcggtgaatg cgaaactgaa
     1921 ccaggagagc cggcgcagca gccagcggac cggagaaatg accgacgtcc gccacctgca
     1981 tttcggggag ccggtaccgg ttaacgcgct ggccgatttt tccacagagc aggcccggaa
     2041 acagcaggcg gtgtttctgg gtaaggatga gcagggccag ccggtgctgg tcccgcgtga
     2101 tacctggcgc aagaccaata ttcagatcct cggcctgccc gggagcggca agagcgtgat
     2161 gggcaccaac gcgctcattc gctgcgtacg cgatttcggg gacgcggtgg tgtacttcga
     2221 ccccaaaggg gacgcgtggg cgccgcacgt cttccgggcg cactgcccgg acttcaccct
     2281 gctggatctg cggccgggga aaccggcgca gctgaatctg tttcgcgatc tcgaccagta
     2341 cgccctgaag aatctgctgg ttgccgggtt taacctgagt gagaccggcg acgtggcgga
     2401 ccactaccgc atcagcgagc agaaagcggc gaagctgatt gcggagcagt tcccgcaggg
     2461 ggcaaacatt cagcaggtgc tggcggcggc atacgcgctg ccggaggccc tgaaaaagga
     2521 cgtgaagggg ctgatcacca agctggagaa cgtggccgac ctgagcgtcc tgcagacgga
     2581 cagcggcatc gacgtggcgg ggataatcaa cggcggcggc tgcctgtacg tcatcggctc
     2641 gatggacgat gaggcggtga tccgggtgca gaagatgctg tttgcccgct gcgcgcaaat
     2701 catcattgcg cgggatgagt tccggaggtg gccgcacgcg agcatcatgc tcgatgagat
     2761 taaatacctg ctgtcgaagt acgtgctgaa cgcgctgggg acgctgcgca gtcgggactg
     2821 caacctgctg ctcgcgcacc agtcgctggg cgacttcggg cagtgcggcc aggatctgcc
     2881 cgctgagttt gtgaaaacca cggtgctgga taacacgccg atccgctggt tctaccgggc
     2941 ggccagccag gagtccgcgc agtgggcggc gggccagacc ggggaaatcc gggtggacgt
     3001 ggagcgccgc cgggccagcc gggaggcggg gaacgtggag catatcagcg gggacagctt
     3061 cattcagaag gaggcgcggc cgctgtttga cgtgaacacc ctgcagcacc tgcctgacgg
     3121 gtttgcggtg atgaccgggc tgggcgtggc ccggatggcg ttcagcagcc cgctgcgggt
     3181 tgaacgccgg gaaatcccgc tgcagcgctt cccggtgctg gcgaaaacgg acccgctggc
     3241 ggagtaccag ccggaggccg ggcggcagcg gccgggtgat gatgattttg cggggctgta
     3301 ctgatggcac tggagcgata caacgtcagc cacgcgaagc ggcaggcgcg gaacgcggag
     3361 aaaacccggc tgaccctgcg ctggctgcgg gaagagctgt gttccacggc ggagctggtt
     3421 gcgcgacggc tggggattgc ggcggtgcag ccggtttacc gcttcctgga cagcctggtg
     3481 gcgaaagggc tgctggtccg ggcgaaatac ccggttgacg gccgtcaggt cagcgtgtgg
     3541 ggactgacgc cgcacggggt ggcgttctcg ttcgacgagg acgaaccgct gacggacgtt
     3601 attccgtttc agccgtcaag ggtgtcggcg gcgcagctgc cgcaccggct ggccgtgcag
     3661 tccctgcgtc tcgccatgga ggcccggggg gcctccggct ggcggtacct gcaccgtatg
     3721 gccctgaagg ggatgaaggt gccggacgcg ctggcggagc tggacggcag aacggtggcg
     3781 tttgaggtgg agcgcaccgt gaagagccgc aggcgctatc aggaggtggt gtccgggtat
     3841 ctgttcaatc gcaaagcgaa cggcatcgat gaaatctggt acatctgccc ggaccgggcg
     3901 acgcaggtca gggtgcagcg ggcgatcctg tcggtggatg aaatcgtgaa tccacagacc
     3961 ggcgaggccc gcaaaacggc ggagctggac cgggagcggc tgtttgcgtg ctttaaattt
     4021 atgacaacgg agtgagggat tatggcgctg tacatggtat ttattgaaga cgcggaggac
     4081 cgggacggcg agcgctgcat catgccgacc agcgagcatc ttgaagatat ggtggtgctc
     4141 gcctggcaga ccggggcgta tgttttctgt tttgtcgacc gggcccagca tctggcgatg
     4201 atgaaatcca tcgggatgta tgacgagcgg ctcaacagcg acgaaaaagc cacgtacttt
     4261 ttcaccggta aaaatccgca atgggaacgg gacaaacccg caggaatgaa gctgtgtgat
     4321 tacctctatc gcaaggtgga gcggttcgcc atcgagaacg gtgtgcaggt ggttgatgcc
     4381 gggaagtgct caccggagca gattagcgcg atgattgagg tggtcagcga gcggcacggg
     4441 gaaagcgcgg tgaacgagaa gctgaaagcg gagctgatgc gggaatataa cagccgctat
     4501 tttaacgtgc cggtgcctga actgccggag gacaaacccg cccgccggtg gtggccgttc
     4561 ggcaaaaaat aaacggcagc accggccatg ccggtgctgt tattccttcc cgagcttatc
     4621 ccgcatcacc tgccagagtg tttccgcatc ctcgtgcagc cgctcgcaca tgtcggccgg
     4681 ggtatccata tccagctcgt tcagtttctc gagtaacagc agcgactggt ccttaataaa
     4741 tttagccata ttgagggcag ttttttcctg cttagtcatg ttttctgtcc ttccgggtgt
     4801 ggtgtgcttc ctgtcgggta tactgtctca aaaccggagg tgagacaatg gcgtgggtga
     4861 cggtgagaca ggcgacggaa ctgacgggga aagcccggag ttccctgtat agggatatgg
     4921 cgaaaggccg cgtatcatac cgtactgaag ctgatggtgg tcgggtggtg gataccagcg
     4981 aacttatccg ggtgtacggt gaattaagac agattgagac tcacgagcgg gacgggttga
     5041 gactgtcgga tgagactgaa aaaatggcct ccgacgcgtt gatcgcggag ataaaagcac
     5101 tcagggagga ggtggccgga ctgcggcagg aaatgcaggc catgagactg ctggaacaca
     5161 aaccagagac agcagagaca ccggccggaa catcgcgccg atggtggcag tggggaaaac
     5221 gctgacgcgc tgtgcttgtc caaccggcga cggccacgca atgaattgcg cccccatcac
     5281 cggttatcag cgaacggtat ggggattctg gcgctggcca accacggcca gaacccggac
     5341 tttcctggtc tggtcatcaa catcatagag gacatgctgt cccatcagac tgatccggcg
     5401 aacgtcctcg ccatagcgtg agtccacttc atataggcgc gggaagtcgg ccagtttgtc
     5461 cgctgcagcg agaactcgat cggtaacgtt ctgcgcatcc cacagggatg aattttccgc
     5521 aatgtagtca aacaatgctg tcacatcctc gatggctccc tgcttccatt ccacctcata
     5581 cggcatcgcg tagttcctga tagcggccat gcagtctctg gcggagttgt tccgtggtca
     5641 tgcccgggcg cgtgtcagcg cgggcggcgg tcactttagc gcgaacctga tcttcatatt
     5701 ctgcctgggc cagttcttcg ttagccaggc gctgcaacag tgcagctttg tccgggccac
     5761 cttcaggcag tatgcggggc agtctctcgg ccagttcccg caggaaattg gcgttttcat
     5821 agtgtgtact gttgctcatt gtggagcctc ccgggctgat acgtcgatat gaaggagttt
     5881 agcagggggc ggtctgaaag aatatgctcg tgtcagtcag gctgatgaag acgaaagctg
     5941 gtaaaaaagg gcttacgaca cccattttac gtcaaactgc acgacatgca gttgcgtgca
     6001 tagccatgca gcgggttaaa atttatcagg cgcgtttccg gcggttttcc gggtggtttg
     6061 ttgccggttt tatcccgtag ccgccggaaa tgccctcaga ccgtctgagc ggtgcgcgta
     6121 atgcgacgtt atggtaaatc atgtgattaa tggtagtgat aatcattatc acttttgcta
     6181 tattcttgtt tcttttattg taaggaaatg taagtatggc aatcacgatt aaatccgccg
     6241 ttaatgtccg tagcagagta ttaatatact ctttactact gtttactggt tcagcgtatg
     6301 cggctgatat agctgaatca acattcctga ctcgtgatat cggcaatggt ctgtatgaaa
     6361 tgtcttttga tggcaaaaat aatagtctct gggcagcatc atcgtcttca ttcgacagag
     6421 ataagggcca cggaattgtt tatcagttag aaccatccac tctgaaaaca aaatctgccg
     6481 ttaaaatcgc acgtcttggg tttgccacag cactggatga ggaacatgag cgactttata
     6541 tcggcaatac tctggacggc tcagtgacgg tcattgatac tctaaccgga aaagaacttt
     6601 cagtaattcc gttaactgag ggacaggatg aggctcattt cagaccgacc agagaaatgg
     6661 tcattgacaa aaaaaatcag cgtctttatg tctccagcgc cgcagataaa ggggctctgt
     6721 gggttattga catggctgtc gggaagaaaa ttaaaaccat tgatggactg gggcacgctg
     6781 ttactggaat tgcgttaaat aaaaatgggg ataatattta tctggttaac ggggatggtg
     6841 agattattaa cctggatgcc agtagctatg aaataaaaaa acggtttgtt gtagagcctg
     6901 agaaaaaaca ctttttcctt aatgtaagtc tggatgaaaa aaacggacgt gcatttatta
     6961 ccgacccgga tattcccggg gtgctggttg ttgatgtaaa ctcgggaaaa atactacaca
     7021 gaattgatgt tataaattca ctggcgattt tatttaatcc tttacggaac gaaatctata
     7081 tcacgcaccg taatgcaaaa caggtcagca ttgtggacag taattcttat tacgtaaaaa
     7141 caacgataaa aacccgcctg atgccaaaca gtctgtcttt atcacatgac ggtgaaaatc
     7201 tgtacgttag tgtaaaacaa gaaaaaaaag agatgagtac tatgaaggat tacatactca
     7261 aaatcgaatt atctaaaatt gctatggagc cagaagaatg aataaatatt tagggctgag
     7321 catagtatat atgatgtata catcatctgt tatggctgat gattctggcg cagttagtaa
     7381 agataaaaat atttcaccag aagaaacatt aattgttcat gggaccgctg tttctgaaag
     7441 cacaacagaa ggaacagatt catacacaac ccagtcagcc aatacagcat caaagatgaa
     7501 tctgtcattc cgggatacac ctcagtcaac tacagtcatt acgcgacgaa gaatggatga
     7561 tgtagcagca aaaaatatta ttgatgtggt taaaaccgct cctggattat atgtcagtac
     7621 agcagaaggt tccggaagac cgaattttat gtccagagga tttaatgcta atgtcatgta
     7681 tgaaggattt agcctgccgt ggaattcata cattccaagt tcacaaacta gttcagctat
     7741 ttttgaccgg gtggagatag tccgcggggc aacaggtctt gttcagggtg ccggagaccc
     7801 ttcagccgcg ataaatatga tacacaagcg tccgacccgg caatatcagg ccagcactaa
     7861 catcggtgcc ggacggtgga atgactaccg gggtggggtt gatgtatcag gaccactgaa
     7921 tgacagcgcg accttacgcg gaagggcagt tgtggatggc gaaaacagtg agaccttcag
     7981 ggatagtgag aaacgcagga ataacgtcat ttacggaatt atggaggcag atctgactga
     8041 taatacccta cttacgattg ggggatatca gcagtatgaa tataacaatc attggtggta
     8101 tgacttacct atttctggta ccggacacca ccttaatctt cctcgctcga cgtttacagg
     8161 caatgactgg gagtttgaaa gaagtaaaag taaaactgca tttgcaactc ttgagcagaa
     8221 gttcagtaat agctggaagc tgaaattttc aacgctgcaa aactggagaa atgctgacct
     8281 gttagggacg gcaacatacc ggcataaaga tacccgacaa agtatgtatc agcaagtatg
     8341 gggatcactg agggaatata attccaaaag ctatgacctg tcgcttaatg gggactatgc
     8401 tttatttggt cgtgttcatc aattcgtggc tggaacaact cagcaaaaat acaccgataa
     8461 agatacaggt tatacatatt ccccaatata tattgagaat gttaatatat ttgatcatgg
     8521 ttcacattct tcgaaaaaac ccatgaagat gtcaggtgat gcatcctacg acgtcactga
     8581 acaaaaaagc gcctacgcag caactcattt tctacttagt gataactgga aaatattgct
     8641 gggtggccgt cttgactggt atgaatatac aagtaaaggt acatccatta atacaggtta
     8701 taaagttacc agaaatatca ccaaatatgc aggttcaaca tacaatcttg acgatcatca
     8761 taccatttat gggagctata ccgatatatt caatccacag tcaagcaccg acctcaatgg
     8821 taaagtgatc aagcctattg ttggtaagaa ttatgagaca ggtattaagg gtgagtattt
     8881 taacggcttg cttaatgcct ctctggcggt atttcaggta gaccaggaaa atcgagccag
     8941 gctgcttccc gatcagacag ggtgtgttac taatggcaat cctcaatgct atgaggctag
     9001 cggtaaagta cgcacaaaag ggatagattt cgaacttcag ggcatggtta cgccactctg
     9061 ggaaattggg gtgggatata catatgcaga tacgcgatat ataaaagatg gagatcgtag
     9121 taatgaaggc aagcgattca atactgccgt gtcgccacaa aatttattta agatttcgag
     9181 catgtacacc tttgacggaa aattaacaaa ctggcgagca ggagtaaaca cgtactggca
     9241 aagtaaaata tatgctaaca taacagacac aagggatggc aatccggtta ttaataaaca
     9301 aaaggcttat tcaacaactg acattattat tggctatact ccaatgccac aactggattt
     9361 acagctcaat ataactaatg tctttgataa ggtttattat acatccatag gtgaatcgat
     9421 tggatatggc acggaaacat atggagaacc aagaaatgtt aaaattagtg caacctatag
     9481 attctaacca tgcggattgg gtcttctcct cggatttgcc cctataaatc cgtcttttgt
     9541 ggttccggcg tacaaccccc tgtttgccac ttcagttgat ggttaatcaa atcatgtaga
     9601 ggatcttctt gagatcctcc atggttcgtc gtaatctttt gccctgtaaa cgaaaaaacc
     9661 acctggggag gtggtttgat cgaaggttaa gtcagttggg gaactgctta accgtggtaa
     9721 ctggctttcg
//
