LOCUS       NZ_CP097231             2095 bp    DNA     circular CON 11-JUN-2024
DEFINITION  Klebsiella pneumoniae strain 5 plasmid p2.1, complete sequence.
ACCESSION   NZ_CP097231
VERSION     NZ_CP097231.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27722567
            Assembly: GCF_023374185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Luo,T.L., Corey,B.W., Snesrud,E., Iovleva,A., McElheny,C.L.,
            Preston,L., Bennett,J.W., McGann,P.T. and Lebreton,F.
  TITLE     IS26-mediated plasmid and toxin-antitoxin gene convergence in
            Klebsiella pneumoniae resulting in antibiotic resistance gene loss
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Luo,T.L., Corey,B.W., Snesrud,E., Iovleva,A., McElheny,C.L.,
            Preston,L., Bennett,J.W., McGann,P.T. and Lebreton,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2022) Bioinformatics, Walter Reed Army Institute
            of Research, 503 Robert Grant Ave, Silver Spring, MD 20910, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Assembly Name                     :: MRSN546122
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 124x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023374185.1-RS_2024_06_11
            Annotation Date                   :: 06/11/2024 05:04:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,668
            CDSs (total)                      :: 5,547
            Genes (coding)                    :: 5,374
            CDSs (with protein)               :: 5,374
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 64 of 173
            Pseudo Genes (incomplete)         :: 103 of 173
            Pseudo Genes (internal stop)      :: 27 of 173
            Pseudo Genes (multiple problems)  :: 18 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097231.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="5"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2.1"
                     /geo_loc_name="USA: Pittsburgh"
                     /lat_lon="40.4406 N 79.9959 W"
                     /collection_date="2015-08-08"
                     /collected_by="UPMC"
                     /genotype="ST14"
     gene            complement(254..493)
                     /locus_tag="M4270_RS28265"
                     /old_locus_tag="M4270_28255"
     CDS             complement(254..493)
                     /locus_tag="M4270_RS28265"
                     /old_locus_tag="M4270_28255"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_074146588.1"
                     /translation="MAKKNKMTVLFCFKHNSSGLLYSRETPDNLTADDKKALYLPTVPT
                     GADFEEFLGSENARLVERMQADPAYSLRFSFRFP"
                     /name="hypothetical protein"
     gene            complement(486..1526)
                     /locus_tag="M4270_RS28270"
                     /old_locus_tag="M4270_28260"
     CDS             complement(486..1526)
                     /locus_tag="M4270_RS28270"
                     /old_locus_tag="M4270_28260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01629.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid replication protein, II/X family"
                     /protein_id="WP_159174367.1"
                     /translation="MIDWITAIVPCFHETPLDSGRITKINSSGEIEWESLTAISVVGSH
                     DSSLRLKTHSINDFGNGTHIYFDGNPVKFLQGHNLFGTDNLIPLLCCVLKKITSIPELG
                     LNPTDFDVRSWEKGDFKLNRVDCTVMFDVGNTANALSWIRQAEMNATLSHRGRGQVTKG
                     STLYFGKSSRRSSVKFYAKGEEFQKHSHAAFLQLPALVDYANRGLRCEVVLRSLELNHR
                     GLSSGSAWDDLLPLQIVNDFIGRINMADITAITSDRLDGLTPSFKNVYELWKCGYDIRS
                     MHSKTAFYRYRKGLMDALGIDIATVQYKQPDNVVPFIRIIEAKPMGVPDWAIGTDLYFD
                     GAVNHG"
                     /name="phage/plasmid replication protein, II/X family"
     gene            complement(1519..1794)
                     /locus_tag="M4270_RS28275"
                     /old_locus_tag="M4270_28265"
     CDS             complement(1519..1794)
                     /locus_tag="M4270_RS28275"
                     /old_locus_tag="M4270_28265"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050885152.1"
                     /translation="MASKRPRTTLLLTDELMTAIRSLSDVSGMTMSSIVVQFLEPSIPV
                     MKQLEEIVLEAKKGAAGNGRAIVGSILEGANKTLAELNREFGELDD"
                     /name="hypothetical protein"
ORIGIN
        1 cgaaaatacc cccacggcat cacctgtagg gcagggctta cgcacctacc ccacaggcaa
       61 cgccttgaag ggggtaaaaa gcggctttac agggcgatgg ctcaccatgt gaaccatcac
      121 ccataactag gatgaatagg gagtgtcgta caaccactaa atcggaaccc tgaagggatc
      181 ccccgattta gcagttgacg agtcggcggc ggcgaacgct gtcagatttc aatctgagag
      241 cgcgaggcgt ctatcaaggg aacctgaaac taaagcgaag gctataagct gggtcagctt
      301 gcatacgctc taccaaacgg gcattttcag aacctaaaaa ctcctcaaaa tcagcgccgg
      361 tgggaacggt aggaagataa agggcttttt tatcatctgc cgttaaatta tcaggcgtct
      421 cgcgggaata aagaaggcca gaggaattat gtttaaagca aaaaagaaca gtcattttgt
      481 tttttttagc catgattaac agccccatca aaatataaat cagtgccaat agcccaatca
      541 ggtacaccca taggcttagc ctctatgatg cgtatgaagg gaacgacatt atcaggctgc
      601 ttgtattgga cagtggcaat atcaataccc aaggcatcca taagaccttt acgataacga
      661 taaaaagcag ttttagagtg catagaccta atatcataac cacacttcca aagctcgtag
      721 acgttcttaa atgaaggggt taaaccatca agcctatcag aagtgatagc agtaatgtca
      781 gccatgttaa tacgtcctat aaaatcattt actatttgca gaggaagcaa gtcatcccat
      841 gccgaaccgg aagaaagacc gcgatgatta agctcaagcg aacgtaaaac aacctcacaa
      901 cgcaaaccac gattagcgta gtcaacgagc gcaggaagct gtaagaaagc tgcgtgagag
      961 tgcttttgga attcctcacc cttagcatag aatttcactg agctacggcg cgacgactta
     1021 ccaaagtata acgtagatcc tttagtaacc tgaccacgcc cacgatgaga aagagtggca
     1081 ttcatctcgg cctgacgaat ccaagacagt gcgttagcag tattgccaac atcaaacata
     1141 acagtacagt caacgcggtt taacttgaaa tccccctttt cccaagaacg aacatcaaaa
     1201 tcagttggat taagtccaag ctcaggaata gaggttattt tcttcaatac gcagcaaagc
     1261 aatggaatta aattatccgt tccaaaaagg ttatggcctt gaaggaattt aactggattc
     1321 ccatcaaaat aaatatgagt cccgttacca aagtcattaa tagaatgggt tttaagtctt
     1381 aatgatgaat catggctacc aacaacagaa atagcggtaa gagactccca ttcaatctca
     1441 ccggatgagt tgattttagt gattcgccca gaatctaaag gggtttcatg gaaacaagga
     1501 acaatagcag tgatccaatc aatcatctaa ctctccaaat tccctgttaa gctcggcaag
     1561 agttttattg gcaccctcaa gaatagaacc aacaatagcc ctaccattac cagcagcacc
     1621 tttcttagcc tccaagacaa tctcctctag ctgcttcata acagggattg atggctctaa
     1681 aaattgaaca acaatcgacg acatggtcat gccagataca tcagaaagag aacgaattgc
     1741 agtcatcaac tcatcagtaa gaagaagcgt ggtgcgcggg cgcttagatg ccatataaaa
     1801 cctcaagtac cgaaatcggg acaaagtgtg cagtggggtg tgaaccgtat gaatcaatac
     1861 taaaggaagg aaaaaagaaa tgcaataagg ggtgagtagt tccgcaatcg ggacaaatgt
     1921 gcagtattac agacactgca catcgagatc ggtaatggcg aggatgcgaa tcagaacggc
     1981 gtatgcctcc tcgcagagct gcgggggcaa tccccgtccc gattcgtgat cgcacgacaa
     2041 accctatcac cctggacaga gttgcaaatc tacaaataag gcataccgag caaaa
//
