LOCUS       NZ_CP097227             2095 bp    DNA     circular CON 11-JUN-2024
DEFINITION  Klebsiella pneumoniae strain 11 plasmid p2.1, complete sequence.
ACCESSION   NZ_CP097227
VERSION     NZ_CP097227.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27722573
            Assembly: GCF_023374205.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Luo,T.L., Corey,B.W., Snesrud,E., Iovleva,A., McElheny,C.L.,
            Preston,L., Bennett,J.W., McGann,P.T. and Lebreton,F.
  TITLE     IS26-mediated plasmid and toxin-antitoxin gene convergence in
            Klebsiella pneumoniae resulting in antibiotic resistance gene loss
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Luo,T.L., Corey,B.W., Snesrud,E., Iovleva,A., McElheny,C.L.,
            Preston,L., Bennett,J.W., McGann,P.T. and Lebreton,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2022) Bioinformatics, Walter Reed Army Institute
            of Research, 503 Robert Grant Ave, Silver Spring, MD 20910, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Assembly Name                     :: MRSN546140
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 102x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023374205.1-RS_2024_06_11
            Annotation Date                   :: 06/11/2024 05:01:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,668
            CDSs (total)                      :: 5,547
            Genes (coding)                    :: 5,378
            CDSs (with protein)               :: 5,378
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 98 of 169
            Pseudo Genes (internal stop)      :: 28 of 169
            Pseudo Genes (multiple problems)  :: 18 of 169
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097227.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="11"
                     /isolation_source="respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2.1"
                     /geo_loc_name="USA: Pittsburgh"
                     /lat_lon="40.4406 N 79.9959 W"
                     /collection_date="2016-06-16"
                     /collected_by="UPMC"
                     /genotype="ST14"
     gene            complement(330..569)
                     /locus_tag="M4271_RS28270"
                     /old_locus_tag="M4271_28270"
     CDS             complement(330..569)
                     /locus_tag="M4271_RS28270"
                     /old_locus_tag="M4271_28270"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_074146588.1"
                     /translation="MAKKNKMTVLFCFKHNSSGLLYSRETPDNLTADDKKALYLPTVPT
                     GADFEEFLGSENARLVERMQADPAYSLRFSFRFP"
                     /name="hypothetical protein"
     gene            complement(562..1602)
                     /locus_tag="M4271_RS28275"
                     /old_locus_tag="M4271_28275"
     CDS             complement(562..1602)
                     /locus_tag="M4271_RS28275"
                     /old_locus_tag="M4271_28275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01629.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid replication protein, II/X family"
                     /protein_id="WP_159174367.1"
                     /translation="MIDWITAIVPCFHETPLDSGRITKINSSGEIEWESLTAISVVGSH
                     DSSLRLKTHSINDFGNGTHIYFDGNPVKFLQGHNLFGTDNLIPLLCCVLKKITSIPELG
                     LNPTDFDVRSWEKGDFKLNRVDCTVMFDVGNTANALSWIRQAEMNATLSHRGRGQVTKG
                     STLYFGKSSRRSSVKFYAKGEEFQKHSHAAFLQLPALVDYANRGLRCEVVLRSLELNHR
                     GLSSGSAWDDLLPLQIVNDFIGRINMADITAITSDRLDGLTPSFKNVYELWKCGYDIRS
                     MHSKTAFYRYRKGLMDALGIDIATVQYKQPDNVVPFIRIIEAKPMGVPDWAIGTDLYFD
                     GAVNHG"
                     /name="phage/plasmid replication protein, II/X family"
     gene            complement(1595..1870)
                     /locus_tag="M4271_RS28280"
                     /old_locus_tag="M4271_28280"
     CDS             complement(1595..1870)
                     /locus_tag="M4271_RS28280"
                     /old_locus_tag="M4271_28280"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050885152.1"
                     /translation="MASKRPRTTLLLTDELMTAIRSLSDVSGMTMSSIVVQFLEPSIPV
                     MKQLEEIVLEAKKGAAGNGRAIVGSILEGANKTLAELNREFGELDD"
                     /name="hypothetical protein"
ORIGIN
        1 cgattcgtga tcgcacgaca aaccctatca ccctggacag agttgcaaat ctacaaataa
       61 ggcataccga gcaaaacgaa aataccccca cggcatcacc tgtagggcag ggcttacgca
      121 cctaccccac aggcaacgcc ttgaaggggg taaaaagcgg ctttacaggg cgatggctca
      181 ccatgtgaac catcacccat aactaggatg aatagggagt gtcgtacaac cactaaatcg
      241 gaaccctgaa gggatccccc gatttagcag ttgacgagtc ggcggcggcg aacgctgtca
      301 gatttcaatc tgagagcgcg aggcgtctat caagggaacc tgaaactaaa gcgaaggcta
      361 taagctgggt cagcttgcat acgctctacc aaacgggcat tttcagaacc taaaaactcc
      421 tcaaaatcag cgccggtggg aacggtagga agataaaggg cttttttatc atctgccgtt
      481 aaattatcag gcgtctcgcg ggaataaaga aggccagagg aattatgttt aaagcaaaaa
      541 agaacagtca ttttgttttt tttagccatg attaacagcc ccatcaaaat ataaatcagt
      601 gccaatagcc caatcaggta cacccatagg cttagcctct atgatgcgta tgaagggaac
      661 gacattatca ggctgcttgt attggacagt ggcaatatca atacccaagg catccataag
      721 acctttacga taacgataaa aagcagtttt agagtgcata gacctaatat cataaccaca
      781 cttccaaagc tcgtagacgt tcttaaatga aggggttaaa ccatcaagcc tatcagaagt
      841 gatagcagta atgtcagcca tgttaatacg tcctataaaa tcatttacta tttgcagagg
      901 aagcaagtca tcccatgccg aaccggaaga aagaccgcga tgattaagct caagcgaacg
      961 taaaacaacc tcacaacgca aaccacgatt agcgtagtca acgagcgcag gaagctgtaa
     1021 gaaagctgcg tgagagtgct tttggaattc ctcaccctta gcatagaatt tcactgagct
     1081 acggcgcgac gacttaccaa agtataacgt agatccttta gtaacctgac cacgcccacg
     1141 atgagaaaga gtggcattca tctcggcctg acgaatccaa gacagtgcgt tagcagtatt
     1201 gccaacatca aacataacag tacagtcaac gcggtttaac ttgaaatccc ccttttccca
     1261 agaacgaaca tcaaaatcag ttggattaag tccaagctca ggaatagagg ttattttctt
     1321 caatacgcag caaagcaatg gaattaaatt atccgttcca aaaaggttat ggccttgaag
     1381 gaatttaact ggattcccat caaaataaat atgagtcccg ttaccaaagt cattaataga
     1441 atgggtttta agtcttaatg atgaatcatg gctaccaaca acagaaatag cggtaagaga
     1501 ctcccattca atctcaccgg atgagttgat tttagtgatt cgcccagaat ctaaaggggt
     1561 ttcatggaaa caaggaacaa tagcagtgat ccaatcaatc atctaactct ccaaattccc
     1621 tgttaagctc ggcaagagtt ttattggcac cctcaagaat agaaccaaca atagccctac
     1681 cattaccagc agcacctttc ttagcctcca agacaatctc ctctagctgc ttcataacag
     1741 ggattgatgg ctctaaaaat tgaacaacaa tcgacgacat ggtcatgcca gatacatcag
     1801 aaagagaacg aattgcagtc atcaactcat cagtaagaag aagcgtggtg cgcgggcgct
     1861 tagatgccat ataaaacctc aagtaccgaa atcgggacaa agtgtgcagt ggggtgtgaa
     1921 ccgtatgaat caatactaaa ggaaggaaaa aagaaatgca ataaggggtg agtagttccg
     1981 caatcgggac aaatgtgcag tattacagac actgcacatc gagatcggta atggcgagga
     2041 tgcgaatcag aacggcgtat gcctcctcgc agagctgcgg gggcaatccc cgtcc
//
