LOCUS       NZ_CP097222             2095 bp    DNA     circular CON 11-JUN-2024
DEFINITION  Klebsiella pneumoniae strain 12 plasmid p2.1, complete sequence.
ACCESSION   NZ_CP097222
VERSION     NZ_CP097222.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27722574
            Assembly: GCF_023374225.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Luo,T.L., Corey,B.W., Snesrud,E., Iovleva,A., McElheny,C.L.,
            Preston,L., Bennett,J.W., McGann,P.T. and Lebreton,F.
  TITLE     IS26-mediated plasmid and toxin-antitoxin gene convergence in
            Klebsiella pneumoniae resulting in antibiotic resistance gene loss
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Luo,T.L., Corey,B.W., Snesrud,E., Iovleva,A., McElheny,C.L.,
            Preston,L., Bennett,J.W., McGann,P.T. and Lebreton,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2022) Bioinformatics, Walter Reed Army Institute
            of Research, 503 Robert Grant Ave, Silver Spring, MD 20910, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Assembly Name                     :: MRSN546195
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 113x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023374225.1-RS_2024_06_11
            Annotation Date                   :: 06/11/2024 05:04:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,407
            CDSs (total)                      :: 5,287
            Genes (coding)                    :: 5,133
            CDSs (with protein)               :: 5,133
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 84 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 18 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097222.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="12"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2.1"
                     /geo_loc_name="USA: Pittsburgh"
                     /lat_lon="40.4406 N 79.9959 W"
                     /collection_date="2017-06-12"
                     /collected_by="UPMC"
                     /genotype="ST14"
     gene            complement(183..422)
                     /locus_tag="M4272_RS26940"
                     /old_locus_tag="M4272_26940"
     CDS             complement(183..422)
                     /locus_tag="M4272_RS26940"
                     /old_locus_tag="M4272_26940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_074146588.1"
                     /translation="MAKKNKMTVLFCFKHNSSGLLYSRETPDNLTADDKKALYLPTVPT
                     GADFEEFLGSENARLVERMQADPAYSLRFSFRFP"
                     /name="hypothetical protein"
     gene            complement(415..1455)
                     /locus_tag="M4272_RS26945"
                     /old_locus_tag="M4272_26945"
     CDS             complement(415..1455)
                     /locus_tag="M4272_RS26945"
                     /old_locus_tag="M4272_26945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01629.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid replication protein, II/X family"
                     /protein_id="WP_159174367.1"
                     /translation="MIDWITAIVPCFHETPLDSGRITKINSSGEIEWESLTAISVVGSH
                     DSSLRLKTHSINDFGNGTHIYFDGNPVKFLQGHNLFGTDNLIPLLCCVLKKITSIPELG
                     LNPTDFDVRSWEKGDFKLNRVDCTVMFDVGNTANALSWIRQAEMNATLSHRGRGQVTKG
                     STLYFGKSSRRSSVKFYAKGEEFQKHSHAAFLQLPALVDYANRGLRCEVVLRSLELNHR
                     GLSSGSAWDDLLPLQIVNDFIGRINMADITAITSDRLDGLTPSFKNVYELWKCGYDIRS
                     MHSKTAFYRYRKGLMDALGIDIATVQYKQPDNVVPFIRIIEAKPMGVPDWAIGTDLYFD
                     GAVNHG"
                     /name="phage/plasmid replication protein, II/X family"
     gene            complement(1448..1723)
                     /locus_tag="M4272_RS26950"
                     /old_locus_tag="M4272_26950"
     CDS             complement(1448..1723)
                     /locus_tag="M4272_RS26950"
                     /old_locus_tag="M4272_26950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050885152.1"
                     /translation="MASKRPRTTLLLTDELMTAIRSLSDVSGMTMSSIVVQFLEPSIPV
                     MKQLEEIVLEAKKGAAGNGRAIVGSILEGANKTLAELNREFGELDD"
                     /name="hypothetical protein"
ORIGIN
        1 gggtaaaaag cggctttaca gggcgatggc tcaccatgtg aaccatcacc cataactagg
       61 atgaataggg agtgtcgtac aaccactaaa tcggaaccct gaagggatcc cccgatttag
      121 cagttgacga gtcggcggcg gcgaacgctg tcagatttca atctgagagc gcgaggcgtc
      181 tatcaaggga acctgaaact aaagcgaagg ctataagctg ggtcagcttg catacgctct
      241 accaaacggg cattttcaga acctaaaaac tcctcaaaat cagcgccggt gggaacggta
      301 ggaagataaa gggctttttt atcatctgcc gttaaattat caggcgtctc gcgggaataa
      361 agaaggccag aggaattatg tttaaagcaa aaaagaacag tcattttgtt ttttttagcc
      421 atgattaaca gccccatcaa aatataaatc agtgccaata gcccaatcag gtacacccat
      481 aggcttagcc tctatgatgc gtatgaaggg aacgacatta tcaggctgct tgtattggac
      541 agtggcaata tcaataccca aggcatccat aagaccttta cgataacgat aaaaagcagt
      601 tttagagtgc atagacctaa tatcataacc acacttccaa agctcgtaga cgttcttaaa
      661 tgaaggggtt aaaccatcaa gcctatcaga agtgatagca gtaatgtcag ccatgttaat
      721 acgtcctata aaatcattta ctatttgcag aggaagcaag tcatcccatg ccgaaccgga
      781 agaaagaccg cgatgattaa gctcaagcga acgtaaaaca acctcacaac gcaaaccacg
      841 attagcgtag tcaacgagcg caggaagctg taagaaagct gcgtgagagt gcttttggaa
      901 ttcctcaccc ttagcataga atttcactga gctacggcgc gacgacttac caaagtataa
      961 cgtagatcct ttagtaacct gaccacgccc acgatgagaa agagtggcat tcatctcggc
     1021 ctgacgaatc caagacagtg cgttagcagt attgccaaca tcaaacataa cagtacagtc
     1081 aacgcggttt aacttgaaat cccccttttc ccaagaacga acatcaaaat cagttggatt
     1141 aagtccaagc tcaggaatag aggttatttt cttcaatacg cagcaaagca atggaattaa
     1201 attatccgtt ccaaaaaggt tatggccttg aaggaattta actggattcc catcaaaata
     1261 aatatgagtc ccgttaccaa agtcattaat agaatgggtt ttaagtctta atgatgaatc
     1321 atggctacca acaacagaaa tagcggtaag agactcccat tcaatctcac cggatgagtt
     1381 gattttagtg attcgcccag aatctaaagg ggtttcatgg aaacaaggaa caatagcagt
     1441 gatccaatca atcatctaac tctccaaatt ccctgttaag ctcggcaaga gttttattgg
     1501 caccctcaag aatagaacca acaatagccc taccattacc agcagcacct ttcttagcct
     1561 ccaagacaat ctcctctagc tgcttcataa cagggattga tggctctaaa aattgaacaa
     1621 caatcgacga catggtcatg ccagatacat cagaaagaga acgaattgca gtcatcaact
     1681 catcagtaag aagaagcgtg gtgcgcgggc gcttagatgc catataaaac ctcaagtacc
     1741 gaaatcggga caaagtgtgc agtggggtgt gaaccgtatg aatcaatact aaaggaagga
     1801 aaaaagaaat gcaataaggg gtgagtagtt ccgcaatcgg gacaaatgtg cagtattaca
     1861 gacactgcac atcgagatcg gtaatggcga ggatgcgaat cagaacggcg tatgcctcct
     1921 cgcagagctg cgggggcaat ccccgtcccg attcgtgatc gcacgacaaa ccctatcacc
     1981 ctggacagag ttgcaaatct acaaataagg cataccgagc aaaacgaaaa tacccccacg
     2041 gcatcacctg tagggcaggg cttacgcacc taccccacag gcaacgcctt gaagg
//
