LOCUS       NZ_CP097217             3555 bp    DNA     circular CON 02-SEP-2023
DEFINITION  Escherichia coli strain BE311 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP097217
VERSION     NZ_CP097217.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28105629
            Assembly: GCF_024662255.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Lu,S., Tao,T., Su,Y., Hu,J., Zhang,L., Wang,G., Li,X. and Guo,X.
  TITLE     Whole Genome Sequencing and CRISPR/Cas9 Gene Editing of
            Enterotoxigenic Escherichia coli BE311 for Fluorescence Labeling and
            Enterotoxin Analyses
  JOURNAL   Int J Mol Sci 23 (14), 7502 (2022)
   PUBMED   35886856
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Guo,X. and Lu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2022) College of Life Science, South-Central Minzu
            University, Hongshan, Wuhan, Hubei 430074, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. Version: 2.1.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 232.81x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/01/2023 17:10:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,844
            CDSs (total)                      :: 4,722
            Genes (coding)                    :: 4,507
            CDSs (with protein)               :: 4,507
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 68 of 215
            Pseudo Genes (incomplete)         :: 150 of 215
            Pseudo Genes (internal stop)      :: 44 of 215
            Pseudo Genes (multiple problems)  :: 46 of 215
            Pseudo Genes (short protein)      :: 4 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097217.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="BE311"
                     /isolation_source="feces of piglets"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="China:Hubei,Wuhan"
                     /collection_date="2018-09-03"
     gene            complement(33..647)
                     /locus_tag="M3O69_RS24090"
                     /old_locus_tag="M3O69_24080"
     CDS             complement(33..647)
                     /locus_tag="M3O69_RS24090"
                     /old_locus_tag="M3O69_24080"
                     /inference="COORDINATES: protein motif:HMM:NF025107.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_052895292.1"
                     /translation="MSTKNAQKEQKKESLITTHWGDDATIHGWTAIPNSLLMLQGDLGI
                     GSTEMCILLNILMHQWPESGESISFPSIGTIASRIGVSKRTIQRGVSNLEALGILTRYQ
                     STRNDPLTNGANIFDSTPLKEYLNKKAKGITISNSNKKKERKKTGITPNIKLPRICPKC
                     LKTKATSHEEIVKFFGVRKTMAGEIINSYCKECRASEKDLF"
                     /name="helix-turn-helix domain-containing protein"
     gene            968..1693
                     /locus_tag="M3O69_RS24095"
                     /old_locus_tag="M3O69_24085"
     CDS             968..1693
                     /locus_tag="M3O69_RS24095"
                     /old_locus_tag="M3O69_24085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_001427890.1"
                     /translation="MASMETEQQPQQTTEQIEVKTKRVQCFIDGYNLYHAIEETGNKAL
                     HWVDLKSLCKRYLPKNASIDNVFWFSAKPSHLSRAINENYQNYTNALTECGVDVIGGRF
                     KKKNVTCEADNGCGGVFHKHEEKESDVNLAISLVSGACNDEYDIAIIITADSDLCPPIK
                     YVRERFPNKELWLVAPPGRIRRATDLCSLSSRSMEIKNDALRACCMRDEFYKDGVLVAR
                     RPQEWRAVANTRQRRARSR"
                     /name="NYN domain-containing protein"
     gene            complement(1898..2011)
                     /locus_tag="M3O69_RS24245"
     CDS             complement(1898..2011)
                     /locus_tag="M3O69_RS24245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001712890.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHDLTPLAAAIGN"
                     /name="hypothetical protein"
     gene            complement(2499..2693)
                     /locus_tag="M3O69_RS24100"
                     /old_locus_tag="M3O69_24090"
     CDS             complement(2499..2693)
                     /locus_tag="M3O69_RS24100"
                     /old_locus_tag="M3O69_24090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_052895291.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLRLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENRTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2977..3300
                     /gene="mobA"
                     /locus_tag="M3O69_RS24105"
                     /old_locus_tag="M3O69_24095"
     CDS             2977..3300
                     /gene="mobA"
                     /locus_tag="M3O69_RS24105"
                     /old_locus_tag="M3O69_24095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     Typing          2729..2968
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.6e-123"
     Typing          complement(1940..2464)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.274"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          2978..3297
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.86e-62"
ORIGIN
        1 ggcagttggc catataaaaa atggtcaact gtttaaaata gatctttttc agaagctcgg
       61 cattctttgc aatatgaatt tattatttcc ccagccattg tttttcgcac gccaaaaaat
      121 ttaacaattt cttcgtggct ggttgcttta gttttcaaac attttgggca aatgcgtggt
      181 aattttatgt tgggtgtaat tccggttttc tttctttctt tctttttgtt gctgttgcta
      241 attgttatgc ctttggcttt tttgtttaaa tattctttta gaggagttga gtcgaatata
      301 ttagcgccat tggttagagg gtcattcctt gttgattggt atctagttaa tatccccaat
      361 gcctctaggt tactaacccc gcgttgaatt gttcttttag atacacctat tcttgatgca
      421 atggtaccaa ttgacggaaa agatattgac tcccctgact caggccattg atgcatcaat
      481 atattcagca gaatacacat ctcagtactc ccaataccaa gatcaccttg cagcataagg
      541 aggctatttg gtatagccgt ccacccatgt atggttgcat catctcccca gtgagtagtg
      601 attaatgact cttttttttg ttctttctgt gcgttttttg tgctcataga tgcttttctc
      661 gcgaaaaaaa aatcaaaatg tgaacagtta ctacctaact actcttatat taagagtagt
      721 taggtagtaa catgtatctt ttaggatgac ataaatgtca tccatttgct ctttttttat
      781 atagaactta agttgagtcc actacctaag agttatgctc ttagtggcta tccaagtcga
      841 ggcttggaag cgcattgtac aaaattcttc ctgtattgga tgcgtccata cagtggtctg
      901 ttaactgcgg ttaagagacg caaaaaggcc tgcatatgca ggcctttttg ctatctagag
      961 gtcaaaaatg gcaagtatgg aaacagagca gcaaccacaa caaactactg agcaaatcga
     1021 agtaaaaaca aagcgagtgc agtgctttat tgatgggtat aatttatatc atgcaattga
     1081 agagactggt aataaagctc ttcattgggt tgatcttaaa tctttatgca agagatacct
     1141 tcctaaaaat gcatctattg ataatgtttt ttggttttcc gctaagccat cacatttatc
     1201 aagagccata aatgaaaatt atcaaaatta caccaacgct ttaaccgaat gtggtgttga
     1261 tgttatcggt ggacgtttta aaaaaaagaa tgtaacgtgc gaagctgata atggatgtgg
     1321 tggagttttc cataagcatg aagaaaaaga gtccgatgtt aatttggcta taagtctagt
     1381 gtctggtgcg tgtaatgatg agtatgatat agccattatt atcactgcgg attctgactt
     1441 atgcccccct attaaatacg taagagagcg attccctaat aaagagcttt ggcttgtagc
     1501 tcctccgggg agaatcaggc gagcgacaga tctctgctct ctgtcatcga gaagtatgga
     1561 gatcaagaat gatgctttga gagcctgctg tatgcgtgat gagttctaca aagatggagt
     1621 acttgtagcc agaaggcctc aggaatggcg agctgttgcc aataccaggc aaagaagggc
     1681 tagaagtcgt taggctttgt tgacgcagct agcgcaagga gattaatcta actaggttga
     1741 aaacgaaaaa accgccttgc cgggcggttt ttcgaaggtt catagagttg gcgctctttg
     1801 aaccgaggta actggctttg aggagcacag tcgccaaaac ttgttctttc agtttagcct
     1861 tatcaggtgc ataacttcaa gactcaactc ctctaaatca gttaccaatg gctgctgcca
     1921 gtggcgttaa gtcgtgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc
     1981 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2041 ccgaactgag ataccaacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2101 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2161 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2221 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacggc atcggcgctg
     2281 gcttgttccg gtgctttgct ttttgctcac atgttctttc cggcgttatc ccctgattct
     2341 gtggataacc gtattaccgc ctttgagtga gctgacaccg ctcgccgcag tcgaacgacc
     2401 gagcgtagcg agtcagtgag cgaggaagcg gaagagcgtc tgacgtggca ttttctcctt
     2461 acgctccgct atgccgttct gcatcctgtc cgggtgcgtt atatcccggt gcgattttcc
     2521 gcttcaaagc gtgtctgtat gctgttctga agttcttccg cgagttcgtg cagcttctca
     2581 cacatggcgg cctgttcgtc agcatcaagt gcgtccagtt tttcgagcag cctcaggctc
     2641 tggcttttta tgaatcccgc catgttgagt acggtttgct gctgcttgtt catctttctg
     2701 ttttctccgt tctttctgtc atctgcgttg tgtgattata tcgagtacca cttttcgact
     2761 gttttaccgc cggtattctg ctgtctgact ctttgacggg catttctgtc agacaacact
     2821 gtcactgcca aaaaactgcc gtgcttttgt cggtaattcg agcttgctga caggacagga
     2881 tgtgcaattg ttataccgcg catacatgca cgctattaca attaccctgg tcagggcttt
     2941 gccccgacac ccatgtcaga tacggagcca tgttttatga caaaacgaag tggaagtaat
     3001 acgcgcaggc gggctatcag tcgccctgtt cgtctgacgg cagaagaaga ccaggaaatc
     3061 agaaaaaggg ctgctgaatg cggcaagacc gtttccggtt ttttacgggc ggcagctctc
     3121 ggtaagaaag ttaactcact gactgatgat cgggtactga aagaagttat gaggctgggg
     3181 gcgttacaga aaaaactctt tatcgacggc aagcgtgtcg gggacagaga gtatgcggag
     3241 gtgctgatag ctattacgga gtatcaccgg gccctgttat ccaggcttat ggcagattag
     3301 cttcccggag ggaaactgtc gaaaacagac ggtatgaacg ccgtaagccc ccaaaccgat
     3361 cgccattcac tttcatacat agctatgcag tgagctgaaa gcgatcctgc cgcatttttc
     3421 cggtttaccc cggggaaaac atctattttt gcggtgtttg cggcagaatc gcgttcagcg
     3481 cgtttctgcg gtgcgcgtaa tgagacgtta tggtaaatgg gagcgttaga aacagtccca
     3541 tttacttgag tgggg
//
