LOCUS       NZ_CP097216             4668 bp    DNA     circular CON 02-SEP-2023
DEFINITION  Escherichia coli strain BE311 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP097216
VERSION     NZ_CP097216.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28105629
            Assembly: GCF_024662255.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Lu,S., Tao,T., Su,Y., Hu,J., Zhang,L., Wang,G., Li,X. and Guo,X.
  TITLE     Whole Genome Sequencing and CRISPR/Cas9 Gene Editing of
            Enterotoxigenic Escherichia coli BE311 for Fluorescence Labeling and
            Enterotoxin Analyses
  JOURNAL   Int J Mol Sci 23 (14), 7502 (2022)
   PUBMED   35886856
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Guo,X. and Lu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2022) College of Life Science, South-Central Minzu
            University, Hongshan, Wuhan, Hubei 430074, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. Version: 2.1.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 232.81x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/01/2023 17:10:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,844
            CDSs (total)                      :: 4,722
            Genes (coding)                    :: 4,507
            CDSs (with protein)               :: 4,507
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 68 of 215
            Pseudo Genes (incomplete)         :: 150 of 215
            Pseudo Genes (internal stop)      :: 44 of 215
            Pseudo Genes (multiple problems)  :: 46 of 215
            Pseudo Genes (short protein)      :: 4 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097216.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="BE311"
                     /isolation_source="feces of piglets"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="China:Hubei,Wuhan"
                     /collection_date="2018-09-03"
     gene            complement(join(4447..4668,1..579))
                     /gene="faeG"
                     /locus_tag="M3O69_RS24060"
                     /old_locus_tag="M3O69_24050"
     CDS             complement(join(4447..4668,1..579))
                     /gene="faeG"
                     /locus_tag="M3O69_RS24060"
                     /old_locus_tag="M3O69_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063096999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria major subunit/adhesin FaeG"
                     /protein_id="WP_257776221.1"
                     /translation="MAHAWMTGDFNGSVDIGGSITADDYRQKWEWKVGTGLNGFGNVLN
                     DLTNGGTKLTITVTGNKPILLGRTKEAFATPVTGGVDGIPHIAFTDYEGASVVLRNPDG
                     ETNKKGLAYFVLPMKNAEGTKVGSVKVNASYAGVLGRGGVTSADGELLSLFADGLSSIF
                     YGGLPRGSELSAGSAAAARTKLFGSLSRDDILGQIQRVNANITSLVDVAGSYRENMEYT
                     DGTVVSAAYALGIANGQTIEATFNQAVTTSTQWSAPLNVAITYY"
                     /name="faeG"
     gene            complement(<598..>978)
                     /gene="ompA"
                     /locus_tag="M3O69_RS24065"
                     /old_locus_tag="M3O69_24055"
                     /pseudo=""
     CDS             complement(<598..>978)
                     /gene="ompA"
                     /locus_tag="M3O69_RS24065"
                     /old_locus_tag="M3O69_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /name="ompA"
     gene            complement(<979..1065)
                     /gene="lpp"
                     /locus_tag="M3O69_RS24070"
                     /old_locus_tag="M3O69_24060"
                     /pseudo=""
     CDS             complement(<979..1065)
                     /gene="lpp"
                     /locus_tag="M3O69_RS24070"
                     /old_locus_tag="M3O69_24060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /name="lpp"
     gene            1353..2213
                     /locus_tag="M3O69_RS24075"
                     /old_locus_tag="M3O69_24065"
     CDS             1353..2213
                     /locus_tag="M3O69_RS24075"
                     /old_locus_tag="M3O69_24065"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-116"
                     /protein_id="WP_000027050.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-116"
     gene            complement(3604..4263)
                     /locus_tag="M3O69_RS24080"
                     /old_locus_tag="M3O69_24070"
     CDS             complement(3604..4263)
                     /locus_tag="M3O69_RS24080"
                     /old_locus_tag="M3O69_24070"
                     /EC_number="2.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="WP_000412211.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
                     /name="type A-1 chloramphenicol O-acetyltransferase"
     gene            <4283..4372
                     /locus_tag="M3O69_RS24085"
                     /old_locus_tag="M3O69_24075"
                     /pseudo=""
     CDS             <4283..4372
                     /locus_tag="M3O69_RS24085"
                     /old_locus_tag="M3O69_24075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     ARG             1354..2210
                     /name="blaTEM-116"
                     /gene="blaTEM-116"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-116"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(3608..4263)
                     /name="catA1"
                     /gene="catA1"
                     /gene_name="type A-1 chloramphenicol O-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          3228..3309
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(2648..3208)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.84"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcatctctt gatagacttc caaacaactt tgtgcgcgcc gctgcggcac tcccagccga
       61 gagttcagaa cccctcggca aaccaccata aaagatagag ctcaacccgt cggcaaaaag
      121 cgaaagcagc tccccgtccg cagaagtaac cccacctctc cctaacacac cggcataaga
      181 tgcattcact ttcactgaac caactttagt gccctctgca tttttcatcg gcagaacaaa
      241 atatgctaaa ccttttttat tagtttcacc atcagggttt ctgagtacta cagaagctcc
      301 ttcatagtca gtaaatgcaa tatgaggaat tccatctaca ccaccagtta ctggcgtagc
      361 aaatgcttct ttggttcggc ctaacaaaat tggcttatta ccagtaacag taatggtcag
      421 tttggttcca ccattggtca ggtcattcaa tacattacca aatccattaa gacctgtacc
      481 aactttccat tcccatttct gacgataatc atctgcagtg atactaccac cgatatcgac
      541 cgaaccattg aaatcaccag tcatccaggc atgtgccata ccagatgcag cagatgcagc
      601 aattgccagt gcaatcagag tctttttcat ggatccgttg tccggacgag tgccgatggt
      661 gtgtgcgtca ccgatgttgt tggtccactg gtattccaga cgggtagcga tttcaggagt
      721 gatcgcgtac tcaacaccgc cagcgaagac cggagaaacg ccggtgtcgt ggtttttacc
      781 ataaacgttg gatttagtgt ctgcacgcca taccatgcca cccagacgag tgtagatgtc
      841 caggtcgtca gtgattgggt aacccagttt agcggtcagt tgaacgccct gagctttgta
      901 tgcaccgttt tcaacgctgc ctttgtacgg catacgacct aaccagtcgt aacccatttc
      961 aaagccaaca tacgggttct gatcgatttt agcgttgctg gagcaacctg ccagcagagt
     1021 agaacccagg attaccgcgc ccagtaccag tttagtagct ttcatagtta atttctcctc
     1081 tttaatgaat tctgtgtgaa attgttatcc gctcacaaag caaataaatt ttttatgatt
     1141 tctcgaggtg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     1201 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1261 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1321 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1381 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1441 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     1501 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     1561 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     1621 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     1681 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     1741 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     1801 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     1861 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     1921 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     1981 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     2041 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     2101 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2161 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2221 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2281 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2341 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2401 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2461 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2521 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2581 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2641 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2701 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2761 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2821 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2881 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2941 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3001 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3061 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3121 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     3181 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     3241 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     3301 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     3361 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     3421 accgaaacgc gcgaggcagc tctagagggc ggatttgtcc tactcaggag agcgttcacc
     3481 gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga
     3541 tgcctcaagc tagagagtca ttaccccagg cgtttaaggg caccaataac tgccttaaaa
     3601 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
     3661 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
     3721 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
     3781 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat
     3841 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
     3901 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
     3961 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
     4021 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
     4081 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
     4141 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
     4201 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     4261 cattttagct tccttagctc ctgaaaatct cgccaagcta gcttggattc tcaccaataa
     4321 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
     4381 ctggatctat caacaggagt ccaagctcag ctaattaagc ttggctgcag gtcgacccgg
     4441 ggtaccttag taataagtaa ttgctacgtt cagcggagcg ctccactgag tgctggtagt
     4501 tacagcctga ttaaaagttg cctcaatagt ctgaccgttt gcaataccca gtgcataggc
     4561 agcagaaaca acagttccat cagtgtactc catgttttcc ctgtaagaac ctgcgacgtc
     4621 aacaagagaa gtaatatttg cgtttactct ttgaatctgt ccgagaat
//
