LOCUS       NZ_CP097215             5152 bp    DNA     circular CON 02-SEP-2023
DEFINITION  Escherichia coli strain BE311 plasmid unnamed2, complete sequence.
ACCESSION   NZ_CP097215
VERSION     NZ_CP097215.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28105629
            Assembly: GCF_024662255.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Lu,S., Tao,T., Su,Y., Hu,J., Zhang,L., Wang,G., Li,X. and Guo,X.
  TITLE     Whole Genome Sequencing and CRISPR/Cas9 Gene Editing of
            Enterotoxigenic Escherichia coli BE311 for Fluorescence Labeling and
            Enterotoxin Analyses
  JOURNAL   Int J Mol Sci 23 (14), 7502 (2022)
   PUBMED   35886856
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Guo,X. and Lu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2022) College of Life Science, South-Central Minzu
            University, Hongshan, Wuhan, Hubei 430074, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. Version: 2.1.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 232.81x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/01/2023 17:10:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,844
            CDSs (total)                      :: 4,722
            Genes (coding)                    :: 4,507
            CDSs (with protein)               :: 4,507
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 68 of 215
            Pseudo Genes (incomplete)         :: 150 of 215
            Pseudo Genes (internal stop)      :: 44 of 215
            Pseudo Genes (multiple problems)  :: 46 of 215
            Pseudo Genes (short protein)      :: 4 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097215.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="BE311"
                     /isolation_source="feces of piglets"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="China:Hubei,Wuhan"
                     /collection_date="2018-09-03"
     gene            629..1147
                     /locus_tag="M3O69_RS24025"
                     /old_locus_tag="M3O69_24015"
     CDS             629..1147
                     /locus_tag="M3O69_RS24025"
                     /old_locus_tag="M3O69_24015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067503.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     EKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            1154..1387
                     /locus_tag="M3O69_RS24030"
                     /old_locus_tag="M3O69_24020"
     CDS             1154..1387
                     /locus_tag="M3O69_RS24030"
                     /old_locus_tag="M3O69_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137441.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2207..2608)
                     /locus_tag="M3O69_RS24035"
                     /old_locus_tag="M3O69_24025"
     CDS             complement(2207..2608)
                     /locus_tag="M3O69_RS24035"
                     /old_locus_tag="M3O69_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001505260.1"
                     /translation="MIDIQKLISWLGVEGAKAGLDKSEMTNAELIESFGNLLPKNPSKL
                     KRSDLVEEIILATRRMTHKSVEELMEMSKEDLYSYFHDQKYSRKELLDLLYTLEIRPGS
                     SAKKNLTEFTISEISDIGMYRRVAKGNHA"
                     /name="hypothetical protein"
     gene            complement(2605..3489)
                     /locus_tag="M3O69_RS24040"
                     /old_locus_tag="M3O69_24030"
     CDS             complement(2605..3489)
                     /locus_tag="M3O69_RS24040"
                     /old_locus_tag="M3O69_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505259.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_001505259.1"
                     /translation="MAVTVGFISEKGGVGKTTSCYHIAEGLSRFHEKRVLVIDADYQRG
                     GITGRFFPKLIENFGNQAPQGTTLFNKYQQLYSAAQQTTDIDIIKFNSGIDVIPADPRL
                     STVSTDKLPSTNNIRSNNAILLQHLKTISAVLSPIADNYDYILIDSHPEVSDVMRSIIY
                     ASDYCVSPVKLDRQSSIGVATVIGEINNVNEDIEMLRKALQVGDSYKDTVFAGAMGMMA
                     REYAEELKQTEQYEYNRLRQAGDIFKHYITEGDGLRVAAAERVSVYDVQISNAYKQASQ
                     FKNLTIEFMRTCR"
                     /name="ParA family protein"
     gene            complement(3738..3869)
                     /locus_tag="M3O69_RS24045"
                     /old_locus_tag="M3O69_24035"
     CDS             complement(3738..3869)
                     /locus_tag="M3O69_RS24045"
                     /old_locus_tag="M3O69_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001505258.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPERHDKAPLAAAMSKGVYKIY"
                     /name="hypothetical protein"
     gene            complement(4524..4715)
                     /locus_tag="M3O69_RS24050"
                     /old_locus_tag="M3O69_24040"
     CDS             complement(4524..4715)
                     /locus_tag="M3O69_RS24050"
                     /old_locus_tag="M3O69_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4780..5103
                     /locus_tag="M3O69_RS24055"
                     /old_locus_tag="M3O69_24045"
     CDS             4780..5103
                     /locus_tag="M3O69_RS24055"
                     /old_locus_tag="M3O69_24045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_000955995.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHGAR
                     DDS"
                     /name="plasmid mobilization relaxosome protein MobC"
     Typing          4349..4432
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.07e-36"
     Typing          complement(3798..4338)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.477"
                     /coverage="100.0"
                     /evalue="1.77e-167"
     Typing          2..1491
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.27"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          4751..5100
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="99.0"
                     /evalue="1.08e-63"
ORIGIN
        1 ctgggaaggg agcgtaaccg ggaaggggca acggtgcttc agggtaatcc ggaagaagtc
       61 cgggaactca tcgatgccac gccatttgcg aagaaataca cgtcaggtgt tctgtcgttc
      121 gcggagaagg agctgccgcc gggaggacgt gaaaaggtga tggcgagctt tgagcgtgta
      181 ctgatgcccg gtctcgaaaa aaatcagtac agcatcctgt gggtggagca ccaggacaag
      241 ggacggcttg agctgaattt tgtcattccg aacatggagc tacagagcgg aaaacgcctc
      301 cagccgtact acgaccgggc agacaggccg agaattgatg cctggcagac gctggttaat
      361 caccattacg ggctgcatga ccccaacgcc ccggagaacc gccggatact gactctccct
      421 gataacctgc cggaaacgaa acaggcgctt gcagagagcg tcacacgcgg gatagacgct
      481 ctttatcatg tcggggagat aaaaggccgt caggatgtga ttcaggcgct cacagaggca
      541 gggcttgaag tggtcagggt gacgcgaagc agtatcagca ttgcagaccc gaacggcggg
      601 aagaatatca gactgaaagg agcattctat gagcaatctt ttacagacgg gcgcggagtt
      661 cgagaaaaag ctgaaagaga gagccgaatc tacagagaca atgctgaacg acgagttcag
      721 caagctcgaa aaatctgtaa gcaaggctgt gacatcaaac gagacgaaaa tcaaagacgc
      781 tatagccctg ttcacagctt cgacagagga atcactgaaa aaacaccggg aaggggtgaa
      841 agaggcgatg atgcagcaca ggaaggacgt gttaaagctg gcagggaata cgggcatgat
      901 gttactgggg atggtccttt tcctgtttac cgtgagtggc gggacgctct ggtatcttgg
      961 cgggcggata caggcgaacc tggaagaaat caggatacag gaagaaacat tgcagaaact
     1021 gaacgcgaag acatggggcg tggagtttgt gcaggacggg agaaggaaat ttctggtcct
     1081 tccgtacggg aaatcggcaa cggtgattcc ctatcaggag aaggactggg tacaactgac
     1141 ggagtgacac agagtgacgg agctggaaag acatttgctg aacgccttag agcaactgca
     1201 acaggactat atgcagcggc tgagcgaatg ggagagcgcc ttcgtggaat tgcagaaaat
     1261 gttttcgctt acgcaacggg acaacgcgat gctgaacgag caggtcatgc agttgagtca
     1321 gcaggtgcag cacttgagcg agcggacaga acgcttgagc cagttataca gcgagaacag
     1381 gagataaggg acgaacggct gatacaggag cgcgaacatg agttatccct ggagcgtgag
     1441 cgccagccgg aaatacagga atggacacag gatggccctt cgcttggatg gtaatcagag
     1501 acaggctaaa atgttgtcag cgcgtttccg gtgcattttt tgggataatg gttggttcgg
     1561 gggcaccggg aacgcgcttc agtcagacct tgctgaaaac acaggaacgc gttaaaacgc
     1621 atacgggcgc gattctggtg tgtcttttgt tgctgtgttt tgcctctatt gccagatcgc
     1681 gctcattccc ctgaacttct gttttcccgc tccggtcccc gtcttcttcg cttccgaaag
     1741 catgaaaaaa atccgtttca gctgtctctg gctggcaatt cctgcgtgat tcacatggct
     1801 gcatagctat gcatgtggtt aaaatttacc gggtgcgatc gcggcagttt ttcgggtggt
     1861 ttgttgccat ttttacctgt ctgctgccgt gatcgcaccg aacgcgtttc tgcggtgcgg
     1921 gcaacggatg ttatggtaaa taggttcgtc cggtatgctc aatgaagata agccagtttt
     1981 taactgattc ttttttcgat cgtttgaaac aagaatagta tttggcaata gcggttgtct
     2041 ttatctagcc aagcacaatt agctagatct atgtaaccgt tattttgaga cgatccatat
     2101 ccgatgaccg tcatcgcttt tgattctgac tttaagtaaa ctatatcgcc tatattaaat
     2161 ggcatttttt tatcctataa aaagccacct cacaggtggc ttttatttat gcatgattcc
     2221 ctttcgctac gcgacgatac atgccaatat cacttatctc acttattgtg aactcggtta
     2281 gatttttctt tgctgagctc ccaggtctga tttcaagagt atataacaaa tcaagaagtt
     2341 cttttctgct atatttctga tcgtggaaat atgaatacag atcttctttt gacatttcca
     2401 tcagttcttc aactgattta tgtgtcattc tccttgttgc taaaataatc tcctcaacga
     2461 gatcggagcg ttttaatttg cttgggttct ttggaagtaa attaccaaag gattcaatta
     2521 gctcggcatt agtcatttca cttttatcca agccagcttt agctccctca acgccaagcc
     2581 aagatatcag tttttggata tctatcatcg acaagtcctc ataaactcaa tcgtcaagtt
     2641 cttaaactgt gacgcttgtt tgtaagcgtt acttatctgt acatcataca ctgaaacgcg
     2701 ctctgctgca gctactctta gcccatcacc ttccgttatg tagtgcttga agatgtcacc
     2761 tgcttgccga agcctgttgt attcatattg ctctgtttgc ttcagttctt ctgcatattc
     2821 tctggccatc attcccattg ctccggcgaa aactgtgtcc ttatatgaat cgccgacttg
     2881 cagtgccttt cttagcattt caatatcttc atttacatta tttatctcac ctatgaccgt
     2941 ggccacacca atagatgatt gcctgtctag ttttaccggt gatacacaat aatctgatgc
     3001 gtagattata gagcgcatga catcagaaac ttcggggtgt gaatcaatca atatgtagtc
     3061 ataattatct gcaataggtg ataatacagc gcttatggtt tttaaatgct gtaaaagaat
     3121 ggcattgttc gatcttatat tgtttgtact aggtagctta tcagttgaaa cggttgataa
     3181 gcgagggtcg gctggaatga catcgatacc actattaaat ttaattatgt ctatgtcagt
     3241 cgtttgttgt gccgcgctat ataattgctg atacttgtta aataacgttg ttccttgcgg
     3301 tgcttgattt ccaaagtttt cgatcagttt agggaaaaat cttccagtta tacctccgcg
     3361 ttgataatct gcatctataa ctaaaacacg tttttcatga aaacgactaa gtccttcggc
     3421 aatgtgataa caggacgttg ttttaccaac cccacctttc tcactaataa acccaactgt
     3481 cacagccatt tttattcctt acaaatctct tttttttggt agagggaaca gcgtcaacgc
     3541 tttccttgac gttcagtata acgtcagcgt actcttagcc atcaaacgaa aaaaaccgac
     3601 tggggagtcg gttttttcga gggttcagta agttggggaa cttctgaacc gtggtaacag
     3661 ggtgtacaag accgctgaaa ccaaattctg ttattccagt ttagcctctc taaggctgat
     3721 acttcaagaa ctctcactca gtaaatcttg tacacccctt tactcatggc tgctgccagt
     3781 ggcgctttgt cgtgtctttc cgggttggac tcaagtcgat agttaccgga acaggcgcag
     3841 cagtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg
     3901 gaactgagtg tcaggcgtgg aatgagataa acgcggccat aacagcggaa tgacaccggt
     3961 aaaccgaaag gcaggaacag gagagcgcac gagggagcca ccagggggaa acgcctggta
     4021 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcggtttt tgtgatgctc
     4081 gtcagggggg cggagcctat ggaaaaacgc cagcggcgtg gccttctctg ggttcttgtt
     4141 ttttgctcac atgtttcttt tggtgttatc ccctgattct gtggataacc gcattaccgt
     4201 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgtagcg agtcagtgag
     4261 cgaggaagcg gaagagcgtc ggaagttgca ttttctcctt acacatctgt gcggcatttc
     4321 acacccggca tggcgcactt ttcatacaat ccgcactgat gccgcatggt taagccagta
     4381 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gcaaggtcaa
     4441 cggcctgacg cgccctgacg ggcttgtctg ctcccggcat ccgctcacag acaagctgtg
     4501 accgtctcct ggagctgtat gtgtcagagg ttttcaccgt catccccgaa gcgtgcgagg
     4561 cagctgcggt aaagctcatc ggcgtggtcg tgaagcgatt cacagatgtc tgcctgttca
     4621 tccgcgtcca gttcgttgag cttttccagc agcgttaatg tctggcttct gataaagcgg
     4681 gccatgttaa gggcggtttt ttcctgttta gtcactgatg cctccgtgta aggggggatt
     4741 tctgttcatg gggtaatgat accgatgaaa cgcgagagga tgctcacgat acgggttact
     4801 gatgatgaac atgcacggtt actggaacgc tgtgagggta aacagctggc ggtatggatg
     4861 cggcgggtct gtctgggtga gccggttgcc cgttccggga agctgccgac actggcaccg
     4921 ccgttactgc gccagctggc tgccatcggg aataatctga atcagacggc gaggaaggtg
     4981 aacagcgggc agtggtcttc cggtgaccgg gttcaggtgg tggccgcact gatggccatc
     5041 gagagagagt tgcgcagctt acggcaggtc gtcagggaac atggagcgcg ggatgatagt
     5101 taagtttcat gccaggggaa aaggcggtgg cagtggtccg gttgattacc tg
//
