LOCUS       NZ_CP097197             1934 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain 15-652 plasmid p652-7, complete
            sequence.
ACCESSION   NZ_CP097197
VERSION     NZ_CP097197.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28158754
            Assembly: GCF_028753275.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Wang,Y., Zhou,J., Liu,H., Wang,Q., Zhang,P., Zhu,J., Zhao,D., Wu,X.,
            Yu,Y. and Jiang,Y.
  TITLE     Emergence of high-level colistin resistance mediated by multiple
            determinants, including mcr-1.1, mcr-8.2 and crrB mutations,
            combined with tigecycline resistance in an ST656 Klebsiella
            pneumoniae
  JOURNAL   Front Cell Infect Microbiol 13, 1122532 (2023)
   PUBMED   36779188
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Liu,H., Wang,Y., Jiang,Y. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2022) Department of Infectious Diseases, Zhejiang
            University School of Medicine, Qingchundong 3 RD, Hangzhou, Zhejiang
            310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina; Pacbio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028753275.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 06:01:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,719
            CDSs (total)                      :: 5,607
            Genes (coding)                    :: 5,470
            CDSs (with protein)               :: 5,470
            Genes (RNA)                       :: 112
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 61 of 137
            Pseudo Genes (incomplete)         :: 73 of 137
            Pseudo Genes (internal stop)      :: 17 of 137
            Pseudo Genes (multiple problems)  :: 12 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097197.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="15-652"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p652-7"
                     /geo_loc_name="China:Quzhou"
                     /collection_date="2015-10-20"
     gene            join(1015..1934,1..67)
                     /locus_tag="M4I39_RS28550"
                     /old_locus_tag="M4I39_28520"
     CDS             join(1015..1934,1..67)
                     /locus_tag="M4I39_RS28550"
                     /old_locus_tag="M4I39_28520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_016245711.1"
                     /translation="MQNPKLQNLTDYSPSDAPWDAHKSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGETLNRMNMAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPSMFTRDYVKHDRWVELWRECLRVNYDPNVDVRAVKPRKPKDGE
                     SLASATAELVRGAVAETLKYSTKPADMVADPEWFLELTKQTHKRRFVATGGALKDILKL
                     DQETDADMVIGDDIPEGDDDGSRIAFEWKTESKKYRRSPSKDKAESD"
                     /name="protein rep"
     Typing          complement(1728..1916)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.947"
                     /coverage="98.0"
                     /evalue="1.39e-59"
ORIGIN
        1 tgagtggaaa accgaatcca aaaagtaccg tcgctcaccg tcaaaagata aagctgaatc
       61 agactgacag ggtgtcgggg cgcagccctg accacgacgt ttttgagagg acgccgcttg
      121 tcggcggtac tccaaaaaca catcttgtcc tattggttct actccgatgc aggatagggc
      181 taacgccgca aagtgacgtc actcaattac tgagttcggt ttccgaactc agttgggaca
      241 agatgtgttt ttggtacacc gccatcacga tgtcggtgcc aaaaacgatt cgtcagggct
      301 gcgccccgac accctgccaa cacctcacgc agtcagatta aaatctgcca gcgctcgccg
      361 tttccgaaat cgtcaaactc taaatcgtgg ggtcccgctg gcttgccagt gggttccgat
      421 ttagtgattg acgacattgc tgattgaaaa attggctgag gtggttggat tcacgaagtg
      481 aggccaacca cctggaagac ggtttttaat tgtctggtac cggatacaac cagaacgaat
      541 ggagttggtt ctgtcgtgat gcgaaaaagt atgggttcgc aaatcacaga ctaaatagat
      601 ttacccaatt ccattcgagt gaggttgtta tgttttttga ggttcttaat catgttgata
      661 gtgcgttgag cgttgctgtt tcgctcctgg cgttgtggca gtttttggtg gcgggtgggc
      721 gttgaatggc tggttcgatc cgcgcgcgat ccttgtattt atacttaagg gataactctg
      781 gcgataaaaa atggcatcaa gccttgcgta ccaaggcttc ggggcttatt tgcgttgtag
      841 aaggttttgg aagtattctg ttgggagtgg tcgccttaaa agcgaccccc gcaacgacgg
      901 ccatcgttcg ggggtgtgac cacctgcaga aattcgattc gaagcgacaa ccaacaggtg
      961 atacggcaat gataacacgt caaatgagcc atcgcccagc atgggacggt gtgcatgcaa
     1021 aaccctaaac tccagaacct cacggattac tccccctcag acgcgccctg ggacgctcac
     1081 aaaagcgttt ctgacgacgt aggtgggatt taccttctcg cagccgaata tgagcgctac
     1141 ggggcgcgta tggcttcctg tggcggttta ctgaggtttg gttggtctac cctcaaagaa
     1201 actggagaaa ctcgccttag gctacgtgag gcgcatttct gccgtgtccg gcactgtcct
     1261 gtctgccagt ggaggcgatc cctgatgtgg caagcccgtt tttatcagtc cctgccgcgt
     1321 attgttgcgg attatccaga tgcacgctgg atgttcctga ccctcacagt gcgaaactgc
     1381 gcgatcgggg aattgggtga gacacttaac cgaatgaaca tggccttcca gcgacttaag
     1441 gatcgcaaag agtttcgacc tgttcagggc tggatccgca ccacggaagt tacacgtgga
     1501 agtgacggta gtgcgcaccc gcactttcac actctgatga tggttcctcc ctcaatgttc
     1561 acgcgagatt acgttaaaca tgaccgctgg gtagagcttt ggcgagagtg tttgcgtgtg
     1621 aattacgatc cgaatgttga tgttcgagca gtaaaacccc gcaagcctaa ggacggagaa
     1681 tcattggctt ctgcaactgc ggaacttgta cggggcgcag tggctgaaac cctgaaatat
     1741 tccacgaagc cggctgacat ggttgctgat cctgaatggt tcctcgaatt gactaagcag
     1801 acacataagc gccgatttgt ggctaccggt ggcgcactca aagacatact gaaactcgat
     1861 caggaaactg atgccgatat ggtaatcggg gatgatattc ccgaaggcga tgatgatggt
     1921 tcacgcattg cttt
//
