LOCUS       NZ_CP097196             3809 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain 15-652 plasmid p652-6, complete
            sequence.
ACCESSION   NZ_CP097196
VERSION     NZ_CP097196.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28158754
            Assembly: GCF_028753275.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Wang,Y., Zhou,J., Liu,H., Wang,Q., Zhang,P., Zhu,J., Zhao,D., Wu,X.,
            Yu,Y. and Jiang,Y.
  TITLE     Emergence of high-level colistin resistance mediated by multiple
            determinants, including mcr-1.1, mcr-8.2 and crrB mutations,
            combined with tigecycline resistance in an ST656 Klebsiella
            pneumoniae
  JOURNAL   Front Cell Infect Microbiol 13, 1122532 (2023)
   PUBMED   36779188
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Liu,H., Wang,Y., Jiang,Y. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2022) Department of Infectious Diseases, Zhejiang
            University School of Medicine, Qingchundong 3 RD, Hangzhou, Zhejiang
            310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina; Pacbio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028753275.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 06:01:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,719
            CDSs (total)                      :: 5,607
            Genes (coding)                    :: 5,470
            CDSs (with protein)               :: 5,470
            Genes (RNA)                       :: 112
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 61 of 137
            Pseudo Genes (incomplete)         :: 73 of 137
            Pseudo Genes (internal stop)      :: 17 of 137
            Pseudo Genes (multiple problems)  :: 12 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097196.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="15-652"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p652-6"
                     /geo_loc_name="China:Quzhou"
                     /collection_date="2015-10-20"
     gene            227..310
                     /locus_tag="M4I39_RS28615"
     CDS             227..310
                     /locus_tag="M4I39_RS28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058655973.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHD"
                     /name="hypothetical protein"
     gene            complement(573..1001)
                     /locus_tag="M4I39_RS28520"
                     /old_locus_tag="M4I39_28490"
     CDS             complement(573..1001)
                     /locus_tag="M4I39_RS28520"
                     /old_locus_tag="M4I39_28490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016809951.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRFSAKTLDKIDDFIEHFE
                     KYNLFGDPLKKHPAWIGKVSPSWKVPDHYENKEAIELHARANKLWHAHLGDPVFKDTFH
                     GKYKVSDWVIHFQRLTPNHIKLLELGYHDPMELPQNFE"
                     /name="hypothetical protein"
     gene            complement(965..1135)
                     /locus_tag="M4I39_RS28525"
                     /old_locus_tag="M4I39_28495"
     CDS             complement(965..1135)
                     /locus_tag="M4I39_RS28525"
                     /old_locus_tag="M4I39_28495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_171482495.1"
                     /translation="MKTEQRHDLIKDTEVFGVSLSLLISRANEKSVTMPSGLSREQRRA
                     WAAEQVRKIHS"
                     /name="hypothetical protein"
     gene            1293..1721
                     /locus_tag="M4I39_RS28530"
                     /old_locus_tag="M4I39_28500"
     CDS             1293..1721
                     /locus_tag="M4I39_RS28530"
                     /old_locus_tag="M4I39_28500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_016809950.1"
                     /translation="MTYSIEEKWKAGGLSLGWTAIPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDSRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKRPVYTVRKTTT"
                     /name="helix-turn-helix domain-containing protein"
     gene            2005..2205
                     /locus_tag="M4I39_RS28535"
                     /old_locus_tag="M4I39_28505"
     CDS             2005..2205
                     /locus_tag="M4I39_RS28535"
                     /old_locus_tag="M4I39_28505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017802053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="WP_042918523.1"
                     /translation="MFGFGIQGTINKGFFLQMARRDQDAFLQYLYDQGYSAKDISKNFD
                     IPVPSVYNRINAHRGRGPLPN"
                     /name="sigma-70 region 4 domain-containing protein"
     gene            complement(3007..3429)
                     /locus_tag="M4I39_RS28540"
                     /old_locus_tag="M4I39_28510"
     CDS             complement(3007..3429)
                     /locus_tag="M4I39_RS28540"
                     /old_locus_tag="M4I39_28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_016809948.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEYNHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3429..3620)
                     /locus_tag="M4I39_RS28545"
                     /old_locus_tag="M4I39_28515"
     CDS             complement(3429..3620)
                     /locus_tag="M4I39_RS28545"
                     /old_locus_tag="M4I39_28515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_026056049.1"
                     /translation="MTKQQQTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          46..527
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.861"
                     /coverage="94.0"
                     /evalue="1.36e-133"
ORIGIN
        1 ctccgccccc ctgacaagca tcacagaagc ggacgctcaa atcagtggtg gcgaaacccg
       61 acaggactat aaagatacct atcatttctg ataggcggct ccctcgtgcg ctctcctgtt
      121 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttatc tcattccacg
      181 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
      241 cgttcagtcc gaccgctgcg ccttgtccgg taactatcgt cttgagtcca acccggaaag
      301 acacgactaa tcgccattgg caacggccac tggtaagcag gatgtgcgag agatgtattg
      361 agagttcttg aagtggcggc ctaactgagg ctacactgga aggacggatt tggtgacatg
      421 tctcgcacaa gactgttacc acggttagat aatctgccag gattaaataa ccttcgaaaa
      481 accacctgcc agggtggttt tttcgttttc acaacagtga gattacgacg aggttgaatg
      541 aatctcaaga tttcatttag tctgctaccc gatcattcaa agttttgggg tagttccatc
      601 gggtcatggt atcccagttc taaaagcttt atatggttcg gtgtcagccg ctggaaatgg
      661 ataacccagt cagaaacctt gtatttccca tgaaacgtat ctttgaaaac cggatcgcct
      721 aaatgagcat gccataactt gttagctcta gcgtgcagct caatagcttc tttgttttcg
      781 taatgatcag gcactttcca cgacggcgat actttaccaa tccaagctgg atgttttttt
      841 aaaggatcgc caaagaggtt atatttttcg aaatgctcaa tgaaatcatc gattttatcg
      901 agtgtttttg cagaaaatcg ggatgtgtaa tagagattga aagtctcaga gaacgagaca
      961 atattcaact gtggattttg cgcacctgct ccgctgccca tgctcttctc tgctccctac
     1021 ttagaccaga tggcattgta acagacttct cattcgctct ggaaatcaac agagacaagg
     1081 atacgccaaa tacttcagta tctttaataa gatcatgacg ttgttcggtt ttcatatcgt
     1141 taactccgta tctaattcag acaggaaaaa atcttttttc cgctttcaga gtagtctttt
     1201 gtagttgcac tataatggcg ttgatgtcac cctattcata tgaataatca tataatatat
     1261 acttcaaata tttttgtttt ttaggaaaat caatgactta ctctattgag gaaaaatgga
     1321 aggctggcgg tttgtctctt ggctggactg caatacctac tgtcttgttt tttatacaga
     1381 acgagaaaaa gctaacctcc gtagccttta acactctgct taacctgatc gttcattggt
     1441 ggaatttgca agaatggccg catccatcaa tggaaagcct agccatacgt atgggggttt
     1501 ctgtaaggac agttcagaga gctatcaatg atctggaaaa agctaatctc ttggacaaga
     1561 aaccaacgtc gaagagtgat agccgatatg gaggtcgtaa tatttatgat ctaacaaagc
     1621 tcgttgatta cctggataca atgggcccat cagtagctga acaggttaag aaaccccgtc
     1681 ataaaagacc ggtatatacc gtaagaaaaa caacaactta aaaaagagca attgcactgt
     1741 tcgaaggaaa aacgataact actctatggt attgcttgct gtataggtat tggtggtcct
     1801 gcggctggtt catcgcttca cggttaaaat agtcctgtaa gctgttaata tttaacataa
     1861 cactactacc acgaacctac gtaacaatcg ctaccaacac agaagtgaca aatttataaa
     1921 aaaacaaaaa ccataaaaaa acttgacttt atgttttttt tggctaattt ctttatcatt
     1981 tctttatcaa aaatagaggt ggctatgttt ggttttggaa ttcaaggtac tattaataaa
     2041 ggattttttc ttcagatggc tcgtagagat caggatgcat ttttgcaata tctctatgac
     2101 caagggtact cagcaaaaga tatcagcaag aacttcgata ttccggttcc tagcgtctac
     2161 aacagaatca acgctcaccg aggccgtgga cctcttccca attaatggtt cttctataca
     2221 aaaaagtcgc ctggataggc gactttttta actgaactct gtgtatagat ttaccataac
     2281 gtctcaattg tacgcaccgc taaaacggcc tcagggcgtt tctgacggaa aaccggcaaa
     2341 acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctccgttt ttacggcatg
     2401 catgagtatg cgcacaagtg catagcgcga aaactcacgc cattcagcca gggtaagtcc
     2461 tgtttccggc gttctttcca tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc
     2521 gtgaagcgtt gccggcgcag ccggaaaacc ggttttcagc acgttcagga atcgtgttcg
     2581 tcgacggccg gcaatcacgt tatggcaagt cgccggacga ctcaccacac gattcaccgg
     2641 cgactcttca ctagagtcgt acccagagtc gctgagtcta aaaaacaacc aaacggggag
     2701 tcgggtctag agtcgggtcg ttattaactc attgttttta tagtcctgag tcgttaccga
     2761 ctctatgacg actcgccggg gactctatga cgagtcgtca tagagtcgga tctatgtaaa
     2821 taaaatttaa aaaacgaaat ttcacgaaag taaaccactg atattaaata caaatctgca
     2881 aaattttaat tttgcgtagt gtgtgggtat ttttagtcgt gaggagcgtt cagagatctg
     2941 gcgatttttg ggaaggttca ggaactcccc gggatggccg gagaggaaaa ctgaagcatg
     3001 acggggtcag gattttttcc agaatttcca ccacggggaa tggggtggtg aagaaggttc
     3061 gtgattgtac tccagaagct gcatagcctg acgaagggaa tcaatatgct catctcgagc
     3121 cttaacagcc cctttaagac catcgttttc agcttctagt aaagcaattc tatgttgtaa
     3181 atcatgtaaa ccaacgctgt taacacctgt agattctgcg tcacttttta catcatattt
     3241 tacagtatcg atgacaccat caagagaacc aaacactcta ataagctctg aggtatcaat
     3301 aactttaata gccttcccgt gctttacaac agaggtgtaa cttaacttcc cactgttaat
     3361 catcttgtaa attgtggggc gactaacctt aacaagccgt gccgcttcac taatagacac
     3421 tctggccatc agttaccatc ctgtaattca tccagtctcg aggacagtgt acggaaaaga
     3481 tgctccgcat catcgtggag tttctcacaa aggtcggcct ctacatcgag atcaagctcg
     3541 ttaagcttct ccagcaggag cagagactga ttctggatga acttagccat gtttagcgcc
     3601 gtttgctgtt gtttcgtcat aaagagcctc agagcgtcag tgctcaatgt aaaaactgtc
     3661 tcctttccgc ttcctcgctc actcagtcgc tacgctcctg ccgttcggct gcggcgagcg
     3721 gtcgcaactc acgtataaga gcataagacg acagggctga tagccggagg ggacaaaata
     3781 aggtcgcggc gttgccgttt ttccatagg
//
