LOCUS       NZ_CP097195             3991 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain 15-652 plasmid p652-5, complete
            sequence.
ACCESSION   NZ_CP097195
VERSION     NZ_CP097195.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28158754
            Assembly: GCF_028753275.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Wang,Y., Zhou,J., Liu,H., Wang,Q., Zhang,P., Zhu,J., Zhao,D., Wu,X.,
            Yu,Y. and Jiang,Y.
  TITLE     Emergence of high-level colistin resistance mediated by multiple
            determinants, including mcr-1.1, mcr-8.2 and crrB mutations,
            combined with tigecycline resistance in an ST656 Klebsiella
            pneumoniae
  JOURNAL   Front Cell Infect Microbiol 13, 1122532 (2023)
   PUBMED   36779188
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Liu,H., Wang,Y., Jiang,Y. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2022) Department of Infectious Diseases, Zhejiang
            University School of Medicine, Qingchundong 3 RD, Hangzhou, Zhejiang
            310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina; Pacbio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028753275.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 06:01:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,719
            CDSs (total)                      :: 5,607
            Genes (coding)                    :: 5,470
            CDSs (with protein)               :: 5,470
            Genes (RNA)                       :: 112
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 61 of 137
            Pseudo Genes (incomplete)         :: 73 of 137
            Pseudo Genes (internal stop)      :: 17 of 137
            Pseudo Genes (multiple problems)  :: 12 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097195.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="15-652"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p652-5"
                     /geo_loc_name="China:Quzhou"
                     /collection_date="2015-10-20"
     gene            34..146
                     /locus_tag="M4I39_RS28495"
                     /old_locus_tag="M4I39_28465"
                     /pseudo=""
     CDS             34..146
                     /locus_tag="M4I39_RS28495"
                     /old_locus_tag="M4I39_28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(373..1347)
                     /locus_tag="M4I39_RS28500"
                     /old_locus_tag="M4I39_28470"
     CDS             complement(373..1347)
                     /locus_tag="M4I39_RS28500"
                     /old_locus_tag="M4I39_28470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_114269875.1"
                     /translation="MAFFNLLFVASLICFGGVMGSYSRGCRDSQNKFSKDKDENNKRAS
                     VYRFGIASAFICVPFISSFLHVDYSSIIFPVADGGGTKFIEQILLLISVSGISAYLGYA
                     LLDGLANKVLKEQVDGIDKKQQDLEAEQDEFKDELDRSKELIEQLEMDKKTTKFELGYF
                     KAISAVDKAESMMSIPDEALSVKKKLTEALDAVTESLSLVKREDVAKDDYDKLLVLKAY
                     ILKRLDRIDDALSITDELLMSNEDNPILIYNKACYQYILRRCQADNSDIKDMIRRALTI
                     KVTDPEFIRRQEKIRTKVIGNKDNDLEGLFTDAELEELKVAIK"
                     /name="hypothetical protein"
     gene            complement(1928..2200)
                     /locus_tag="M4I39_RS28505"
                     /old_locus_tag="M4I39_28475"
     CDS             complement(1928..2200)
                     /locus_tag="M4I39_RS28505"
                     /old_locus_tag="M4I39_28475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_019405140.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     gene            complement(3004..3426)
                     /locus_tag="M4I39_RS28510"
                     /old_locus_tag="M4I39_28480"
     CDS             complement(3004..3426)
                     /locus_tag="M4I39_RS28510"
                     /old_locus_tag="M4I39_28480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_040214596.1"
                     /translation="MAIVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3426..3617)
                     /locus_tag="M4I39_RS28515"
                     /old_locus_tag="M4I39_28485"
     CDS             complement(3426..3617)
                     /locus_tag="M4I39_RS28515"
                     /old_locus_tag="M4I39_28485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_040198051.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLDKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2..301
                     /name="rep_cluster_2374"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.505"
                     /coverage="94.0"
                     /evalue="2e-61"
     Typing          3666..3988
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.985"
                     /coverage="92.0"
                     /evalue="2.38e-86"
ORIGIN
        1 tccgggaagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac
       61 tgctgcgccc gttccagtaa ctatctcttg agtccaaccc ggaaagacac gacttatcgc
      121 cattggcagc agccactggt aattgattta gaggagttag tcttgaagtt atgcgccagt
      181 taaggctaaa ctaaacgaac tgttttggtg actgcgctcc tctaagccag ttacctcggt
      241 tcagaaactt gccggttact gaaccttcga aaaaccacct tgccgggtgg ttttttcgtt
      301 tgaggggtag agtacgctgc acctcaaagg atcgtcaagg atctttttgg aattcataga
      361 gctagccact gcctatttga tagccacctt taattcctcc agctcagcgt cagtaaacaa
      421 cccttcaaga tcattatctt tatttcctat gaccttggtt cttatttttt cttgcctgcg
      481 aatgaattca ggatctgtaa ctttaatagt cagagccctg cgaatcatat ccttaatatc
      541 ggagttatca gcttggcaac gacgcaaaat atattgatag caagccttat tgtaaataag
      601 tatagggtta tcctcattac tcataagtaa ctcatccgta attgacagag catcatcaat
      661 acgatcaagt cttttcaaaa tataagcttt aagcactaag agcttgtcgt agtcatcctt
      721 agctacatcc tctcttttaa caagagacaa tgattccgtt acagcatcta atgcctcagt
      781 tagttttttc ttaacagaaa gagcctcatc cggaatggac atcattgatt ctgctttatc
      841 cacagctgaa atggctttaa agtatcctag ctcgaatttt gttgttttct tatccatttc
      901 aagctgttct atcagctcct tgctcctatc aagctcatct ttaaactcat cttgttcagc
      961 ctccaaatct tgttgctttt tatctatacc gtctacttgc tcttttaata ctttgttggc
     1021 aagaccatcc agtaatgcgt acccaagata cgcagagatc cccgaaacag aaataagaag
     1081 caatatttgt tctatgaact tagttccacc accatcagcc acaggaaata ttatacttga
     1141 gtaatcaaca tgaagaaatg atgatatgaa aggaacacaa ataaatgcgg atgctatacc
     1201 aaatctataa acactagctc ttttattatt ctcatcctta tcttttgaaa atttattttg
     1261 ggagtctcgg caacctctgg aatagctccc cataacacca ccaaaacaaa ttagagatgc
     1321 aacaaacaat aggttaaaaa aagccatgag agaatccttg cgcaaagcaa acaataaaac
     1381 tttgttcagg taagttaaga taagctcaat catatactaa ccaataacaa aaaaggcagc
     1441 cttggctgcc tttttctgct gacttgacac ttacaagtct ggacgctcat cgtgaagttt
     1501 gatttttttc atagagcacc tctcattaat tgttcctttg caagcccatg cttgcttagc
     1561 gctgaatata gggcataaac ccagcactca caacaacaaa aatgcttaaa atgataaaat
     1621 caacccatgt attgacattg ggtgaaaaaa aaagtataat gcactggtag ccaaaggggc
     1681 ggggcttcgc ttattcctta ggaataagcg aagccccgcc cctttggcta ccataattaa
     1741 atgattgaat ttaaaggatt tttttacaat aacatccttc ctctcaatag tcatataaac
     1801 atgccccatg tattcgtcat tctggatttt tcaaaagaat gaatgcgtgc atttaccata
     1861 acgcctcatt tcacgcagat atgaaaaagc ccgcacaagg cgggcttcag actcaacaga
     1921 tacggaatca ctctgtgaaa aactgctttg ccatggggtg attctgcatg atctcgtcac
     1981 gttcttccgg gtcaaagcac ccacagacac ccagcagaac cggctctgtc agcattccgg
     2041 cacagcatcc cgtggtgacc tgtattttac ctttccggtc atacccaaaa acggtgtagg
     2101 tcacatcatc ctcatcatag acttcatgac agatggagca tgagtccggg tattttttga
     2161 tgagtgcatt aatttctttt acctgagcgt cagtagccaa aacatctccc tgagtcactg
     2221 attatcacaa tccgctgact ctaacacaga ctcaggcatc cgccgttgat ttaccataac
     2281 gtcccattac acgcaccgct aaaacggcct cagaacgatt ctggggcgta acccattaaa
     2341 tcctacgtct taccccctga aaacgcctca aaatcgctcc agggcgtttt acgggcatga
     2401 atcgttatgc acttgactga ctgtctgggg atttcagcct gaattcaggc caggcaactc
     2461 aaaacccgca ccaaaccaga ctgaaaacgg tgaacgggtg cgcaatttat tgcgtggccg
     2521 tgaagcgttg ccggcgcagc cggaaaaccg gttttcagca cgttcaggaa tcgggtttgt
     2581 cgagaccagc aatcacatta tggcaagtcg ccggacgact caccacacga ttcaccggcg
     2641 attcgactcg tgacttgact ctagagtcgc catggtcaaa aagccaccaa cccggcaagt
     2701 cgactctaga gtcaagtcat cataacgtac tgattatatg gcgtgattca catatgaatc
     2761 gtgggcaagt catcggcgat tcgccataga gtcgtccacg actcggattc accttacaaa
     2821 aaataaaatt gcgtaattta aaatagataa ctgattgaaa ataaaggtct atctgcaaaa
     2881 ttttaatttt gcgtagtgtg tgggtatttt tagtggtgag gagcgttcag ggatctgacg
     2941 atttttggga aggttcagga actccccgga atggccggag agcaaaatgg aagcatggcg
     3001 ggatcaggat tttttccaga atttccacca cggggaatgg ggtggtgaag aaggttcgtg
     3061 cttgtgctcc agaagctgca tagcctgacg aagggaatca atatgctcat ctcgagcctt
     3121 aacagcccct ttaagaccat cgttttcagc ttcaagtaaa gcgattctat gttgtaaatc
     3181 atgtaaacca acgctgttaa tacctgtaga ttctgcgtca ctttttacat catattttac
     3241 agtatcgatt acaccatcaa gagaaccaaa cactctaatt agctctgaag tatcaataac
     3301 tttaatagct ttcccgtgct ttaccacaga ggtgtaactt aacttcccac tgttaatcat
     3361 cttgtaaatt gtggggcgac taaccttaac aagccgtgcc gcttcactaa tagacactat
     3421 ggccatcagt taccatcctg taattcatcc agtctcgagg acagtgtacg gaaaagatgc
     3481 tccgcatcat catggagttt ctcacaaagg tcggcctcga catcgagatc aagctcgtta
     3541 agcttatcca gcaggagcag agactgattc tggatgaatt tagccatgtt cagcgctgtt
     3601 ttctgttgtt ttgtcataag aagcctcaga gcgtcagtgc tcaatgtcaa actagtctct
     3661 tttccgcttc ctcgctcact cagtcgctac gctccagccg ttcggctgcg gcgagcggca
     3721 ggagcgtgta cataaaaacg taggcaacca agatatgagg cagaaaagca aaggccgccg
     3781 aacggctgtt tttccatagg ctccgccccc ctgacaagca tcgcggaaac ggacgctcaa
     3841 atcagtggtg gcgaaacccg acaggactta aagataccag gcgtttcccc ctggtggctc
     3901 cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc
     3961 gcgttttctc attccatgcc tgatactcgg c
//
