LOCUS       NZ_CP097085             8802 bp    DNA     circular CON 21-FEB-2024
DEFINITION  Klebsiella pneumoniae strain KP130 plasmid pKP130-3, complete
            sequence.
ACCESSION   NZ_CP097085
VERSION     NZ_CP097085.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28016298
            Assembly: GCF_027558835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Tian,D.
  TITLE     Evolution and transfer of virulence plasmids of hypervirulent and
            carbapenem resistant Klebsiella pneumoniae strains in hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Tian,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2022) Clinical Laboratory, Huashan Hospital, Fudan
            University, No. 12,Wulumuqi Road, Shanghai 200040, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 235x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/21/2024 03:21:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,385
            CDSs (total)                      :: 5,262
            Genes (coding)                    :: 5,129
            CDSs (with protein)               :: 5,129
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 78 of 133
            Pseudo Genes (internal stop)      :: 18 of 133
            Pseudo Genes (multiple problems)  :: 23 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097085.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP130"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP130-3"
                     /geo_loc_name="China: Shanghai"
                     /lat_lon="31.23 N 121.45 E"
                     /collection_date="2017-11-30"
                     /collected_by="Dongxing Tian"
     gene            1..1686
                     /locus_tag="M3I82_RS26840"
                     /old_locus_tag="M3I82_26815"
     CDS             1..1686
                     /locus_tag="M3I82_RS26840"
                     /old_locus_tag="M3I82_26815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_266051493.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQDADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1696..1953
                     /locus_tag="M3I82_RS26845"
                     /old_locus_tag="M3I82_26820"
     CDS             1696..1953
                     /locus_tag="M3I82_RS26845"
                     /old_locus_tag="M3I82_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            2038..2187
                     /locus_tag="M3I82_RS26850"
                     /old_locus_tag="M3I82_26825"
     CDS             2038..2187
                     /locus_tag="M3I82_RS26850"
                     /old_locus_tag="M3I82_26825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3671..5632
                     /locus_tag="M3I82_RS26855"
                     /old_locus_tag="M3I82_26830"
     CDS             3671..5632
                     /locus_tag="M3I82_RS26855"
                     /old_locus_tag="M3I82_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_032448356.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5632..6363
                     /gene="mobC"
                     /locus_tag="M3I82_RS26860"
                     /old_locus_tag="M3I82_26835"
     CDS             5632..6363
                     /gene="mobC"
                     /locus_tag="M3I82_RS26860"
                     /old_locus_tag="M3I82_26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_094313769.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVAQRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMMMD"
                     /name="mobC"
     gene            6392..6655
                     /locus_tag="M3I82_RS26865"
                     /old_locus_tag="M3I82_26840"
     CDS             6392..6655
                     /locus_tag="M3I82_RS26865"
                     /old_locus_tag="M3I82_26840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094313768.1"
                     /translation="MTNRVVAVGREALLMVAVMAGTLVVLAGNLLKLLWRVLPVLLLLY
                     GVVQFLARNPEIRDWLFLHFGQGGTAAILVGGILLCMRRLMK"
                     /name="hypothetical protein"
     gene            complement(6686..6865)
                     /locus_tag="M3I82_RS26870"
                     /old_locus_tag="M3I82_26845"
     CDS             complement(6686..6865)
                     /locus_tag="M3I82_RS26870"
                     /old_locus_tag="M3I82_26845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_094313767.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     EKLWLAMRDHLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6934..7311
                     /locus_tag="M3I82_RS26875"
                     /old_locus_tag="M3I82_26850"
     CDS             6934..7311
                     /locus_tag="M3I82_RS26875"
                     /old_locus_tag="M3I82_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_094313766.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVMDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTAFDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTPRRWWQWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(7373..7672)
                     /locus_tag="M3I82_RS26880"
                     /old_locus_tag="M3I82_26855"
     CDS             complement(7373..7672)
                     /locus_tag="M3I82_RS26880"
                     /old_locus_tag="M3I82_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_094313765.1"
                     /translation="MPYEVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLTAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7662..7925)
                     /locus_tag="M3I82_RS26885"
                     /old_locus_tag="M3I82_26860"
     CDS             complement(7662..7925)
                     /locus_tag="M3I82_RS26885"
                     /old_locus_tag="M3I82_26860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_094313764.1"
                     /translation="MSNTTHYENANFLRELAENLPRILPESGPDKAALLQCLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(8231..8476)
                     /locus_tag="M3I82_RS26890"
                     /old_locus_tag="M3I82_26865"
     CDS             complement(8231..8476)
                     /locus_tag="M3I82_RS26890"
                     /old_locus_tag="M3I82_26865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             1..6686
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2291..2869)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5633..6360
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.004"
                     /coverage="100.0"
                     /evalue="1.97e-140"
ORIGIN
        1 atgagtggtg gagacggtcg aggtccgggt aattcaggtc tgggacataa tggtggtcag
       61 gccagtggga atgtgaacgg tacgtctggt aaaggtggcc cttcatcagg tggtggtacg
      121 gatccaaaca gcgggccggg ctggggtacg acgcatacgc cgaatggaga tattcataac
      181 tataatccgg gggagtttgg taacggcggg agtaaacccg gtggtaatgg tggtaacagc
      241 ggcaatcata gcggtagctc tggtggcgga cagtcttcgg ccactgcgat ggccttcggt
      301 ctgcctgccc tggctactcc gggggctgaa ggactggctt tatcagtttc cggcgatgcg
      361 ttgtcggccg ccgttgctga tgtgctggct gccctgaagg ggccgtttaa gtttggtctg
      421 tgggggattg cgatctacgg tgtgctgcct tctgagattg caaaagatga tccgaaaatg
      481 atgtcaaaaa ttatgacgtc attaccggcc gatgcggtga cggagactcc ggcaagtact
      541 ttaccactgg accaggcgac ggttcgtgtc agacaacggg ttgtggatgt ggtgaaggat
      601 gagcggcagc atattgcggt tgtcgcaggt cggccaatga gtgtccctgt ggtggatgcg
      661 aaaccgacaa aacgtccggg ggtattcagt gtgtcgattc cgggtctccc gtctctgcag
      721 gtgagcgtac cgaaaggtgt tccgacagcg aaagccccgc caaaaggcat tgttgctgaa
      781 aaaggtgatt cacgtccggc tggttttaca gccggtggta actcccgtga ggccgttatt
      841 cgtttcccga aagagaccgg acagaagccg gtttatgtgt cggtgacaga tgttcttacc
      901 ccggcacagg taaaacagcg tcaggaggaa gaaaagcgtc gccagcaggc atgggacgcc
      961 gctcatccgg aagaggggct gaaaagagac tatgataaag cgaaagctga gctggatgcc
     1021 gaagataaaa atattgcgac cttaaacagc cgcattgcat cgacagagaa ggcgctcccc
     1081 ggtgcaaggg ctgctgttca ggacgccgat aaaaaggtaa aagaggcaga ggcaaataaa
     1141 gatgattttg tgacttataa cccgcctcat gaatatggct ccgggtggca ggatcaggtt
     1201 cgctatcttg ataaggatat tcagaatcag aatgagaaat taaaagcggc tcaggcatct
     1261 ttaaacgcaa tgaatgaatc cttatccaga gataaggctg cacttaccgg ggcgatggag
     1321 agccggaaac aaaaggagaa aaaagcgaag gatgcagaaa ataagttaaa tgaggaaaag
     1381 aacaagcctc gcaaggggac taaagactac ggccatgatt atcatccagt acctaaaaca
     1441 gaagacataa agggacttgg agagttaaag cgaggtgatc ctaaaacacc taaacaaggt
     1501 ggtggtggta agcgggcccg atggtatggc gataaaaaac gtaaaattta tgaatgggat
     1561 tcccagcacg gtgagcttga agggtatcgt gccagtgatg gcgaacacct aggcgcattt
     1621 gatcccaaaa cgggtaagca ggttaaaggg ccagatccaa aacggaacat taaaaaatat
     1681 ctttgagagg taaatatggg gcttaaatta cgtttgacat ggtttgataa gaaaactgaa
     1741 gagtttaaag gggaagagta ttctaaagac tttggtgacg atggttctgt cattgaaagc
     1801 ttgggaatgc ctttaaagga taatattaat aatggtgagt ttgatttgaa gaaagaatgg
     1861 gttcctttac tacagccatt ttttaaaaat caaataaaac ctgatgtgga taattgttat
     1921 atagcttttg attatcggga cggtaactgg taacaaaaaa ttatcaagga tgatttgaaa
     1981 tactgacttc cggtgttcag ggatgaatgc cggagtcttt aaatacaggg aaagtatatg
     2041 aaaaaggtaa agactatttt tctttttata ttgattgttg ctggctttct tctggccgca
     2101 tgtcaggcaa attatatccg ggatgttcag ggcggaacgg tggcaccatc gtcctcctct
     2161 gaactgacgg ggatcgcggt tcagtagaaa agatcaaagg atcttcttga gatccttttt
     2221 ttctgcgcgt aatcttttgc cctgtaaacg aaaaaaccac ctggggaggt ggtttgatcg
     2281 aaggttaagt cagttgggga actgcttaac cgtggtaact ggctttcgca gagcacagca
     2341 accaaatctg tccttccagt gtagccggac tttggcgcac acttcaagag caaccgcgtg
     2401 tttagctaaa caaatcctct gcgaactccc agttaccaat ggctgctgcc agtggcgttt
     2461 tgcgtgcttt cccgggttgg actcaagtga acagttaccg gataaggcgc agcagtcggg
     2521 ctgaacgggg agttcttgct tacagcccag cttggagcga acgacctaca ccgagccgag
     2581 ataccagtgt gtgagctatg agaaagcgcc acacttcccg caagggagaa cggcggacag
     2641 gtatccggta aacggcaggg tcggaacagg agagcgcaag agggagcgac ctaccggaaa
     2701 cggtggggat ctttaagtcc tgtcgggttt cgcccgtact gtcagattca tggttgagcc
     2761 tcacggctcc cacagatgca ccgaaaaagc gtctgtttat gtgaactctg gcaggagggc
     2821 ggagcctatg gaaaaacgcc accggcgcgg ccctgccgtt ttgcctcaca tgttagtccc
     2881 ctgcttatcc acggaatctg tgggtaactt tgtatgtgtc cgcagcgccc gccgcagtct
     2941 cacgcccgga gcgtagcgac cgagtgagcg aggaggcgca atttccccgg cacctgcaag
     3001 acggtcaccg gacgcgctga tgtgggatat tgtcccctga ttaagtcttt ctattccctg
     3061 acgatcaatt atcaggatag gtgaaccatg tcggtttttc gctgaacatt ttacgttaac
     3121 ctatctctgt tgcttttttc tccttgcgga gaaatgcaag tcatccccgc tggggcgtgg
     3181 ctgacttcag gggaaaagac cttgcttacg ctggctggac gccaccgacg cacggtcttt
     3241 tcccctcttt ctggctctgt ccggctggac gccggccacg ccagaaattc acccggcccc
     3301 cgtcaggctc acgcctgacc acccacaaaa atccacggtg ctggacgcac ctgacgaagc
     3361 agcaaagctg ctcctgattt tgcgggtggt cagccgctcg ctatggcgaa ctctgacggg
     3421 ggccggaaca ccggaaaaga ccgcactggg gtgccgcctt ttcctctcac acgctggggc
     3481 gtgctcgttg ttcctccggg gctgcgcctt ttccgcatgg ggcggaaaga ggctggcctc
     3541 cgcggtgaaa gctgaacgca atcaccacgg acgtgaccgc cgccgccatg ccttggggca
     3601 tgtccgcggc ttgtgcagcg ggggctgcgc atcacgccgg taaacccgtc gtctgaagga
     3661 gagagattgc atgtttaata cggattcgct tgcctggcag tggcatatcc cggagctggc
     3721 ccggcagatt tacccgtggg tcagctggct gtaccgggag tattacagtc tggccggttt
     3781 ttttctggtt atcggcctgt ttttcggtct ggcagaactc tgggccgggc ggatgcgatt
     3841 cgtggggctg agtcataagg agcggcggga gtatgccgat cgctataagc gcgataataa
     3901 ccggactgcg ccattttcgg tgctggcctc gctggattcg cccgggcgga tggtcagggc
     3961 acggcgacat gtctggaact acgcatactg gctgaccggc gctgccggtc tggcccttct
     4021 gggggggctg gttgtcgccg tgtttatcga gagctgggga gaaaccgtcc tgcgtccgga
     4081 cctctacgcg aacgcagatt attacctgct gcagccggct aacctgttcg ggattgcctg
     4141 gtgtgccgga ggtcttgtcg caggcgtcct cctggcgcgg ctgctggcgt tcctgctgct
     4201 tgacgggcat tttgcggccg ctgacgaggc ggtgaacgcg aaactgaatc aggagagccg
     4261 gcgcagcagc cagcggaccg gagaaatgac cgacgtccgc cacctgcatt tcggggagcc
     4321 ggtaccggtt aacgcgctgg ccgatttttc cacggagcag gcccggaaac agcaggcggt
     4381 gtttctgggt aaggatgagc agggccagcc ggtgctggtc ccgcgtgata cctggcgcaa
     4441 gaccaacatt cagatcctcg gcctgcccgg gagcggcaag agcgtgatgg ccaccaacgc
     4501 gctcattcgc tgcgtacgcg atttcgggga cgcggtggtg tacttcgacc cgaaagggga
     4561 cgcgtgggcg ccgcacgtct tccgggcgca ctgcccggac ttcaccctgc tggatctgcg
     4621 gccggggaaa ccggcgcagc tgaatctgtt ccgcgatctc gaccagtacg ccctgaagaa
     4681 tcttctggtt gccggattta acctgagtga gaccggcgac gtggcggacc actaccgcat
     4741 cagcgagcag aaagcggcga agctgattgc ggagcagttt ccgcaggggg caaatattca
     4801 gcaggtgctg gcggcggcgt acgcactgcc ggaggccctg aaaaaggacg tgaaggggct
     4861 gatcaccaag ctggagaacg tggccgacct gagcgtcctt cagacggaca gcggcatcga
     4921 cgtggcgggg ataatcaacg gcggcggctg cctgtacgtc atcggctcga tggacgatga
     4981 ggcggtgatc cgggtgcaga agatgctgtt tgcccgctgc gcgcaaatca tcattgcgcg
     5041 ggacgagttc cggaggtggc cgcacgcgag catcatgctc gatgagatta aatatctgct
     5101 gtcgaagtac gtgctgaacg cgctggggac gctgcgcagc cgggactgca acctgctgct
     5161 cgcgcaccag tcgctgggcg acttcggcca gtgcgggcag gatctgcccg ctgattttgt
     5221 gaaaaccacg gtactggata acacgccgat ccgctggttc taccgggcgg ccagccagga
     5281 gtccgctcag tgggcggccg gacagaccgg ggagatccgg gtggacgtgg agcgccgccg
     5341 ggccagccgg gaggcgggga acgtggagca tatcagcggg gacagcttca tccagaagga
     5401 ggcgcggccg ctgtttgacg tgaacaccct gcaacatctg cctgacgggt tcgcggtgat
     5461 gaccgggctg ggcgtggcgc ggctggcgtt cagcagcccg ctgcgtgttg atcgccggga
     5521 aattccgctg aaaaccttcc ctgtgctggc gaaaacggac ccgctggcgg agtaccagtc
     5581 ggaggccggg cggcagcggc cgggtgatga tgattttgcg gggctgtact gatggcactg
     5641 gagcgataca acgtcagcca cgcgaagcgg caggcacgga acgcggagaa aacacggctg
     5701 accctgcgct ggctgcggga agaactgtgt tccacggcgg agctggttgc gcaacggctg
     5761 gggattgcgg cggtacagcc ggtttaccgc ttcctggaca gcctggtggc gaaagggctg
     5821 ctggtccggg cgaaataccc ggttgacggc cgtcaggtca gcgtgtgggg gctgacgccg
     5881 cacggggtgg cgttctcgtt cgacgaggac gagccgctga cggacattat tccgtttcag
     5941 ccgtcgcggg tgtcggcggc gcagctgccg caccggctgg ccgtgcagtc cctgcgcctc
     6001 gccatggagg cccggggggc caccggctgg cggtacctgc accgtattgc cctgaaaggg
     6061 atgaaggtgc cggacgcgct ggcggagctg gacggcagaa cggtggcgtt tgaggtggaa
     6121 cgcaccgtga agagccgcag gcgctatcag gaggtggtgt ccgggtatct gtttaaccgc
     6181 cgggcgaacg gcatcgatga aatctggtac atttgcccgg accgggcgac gcaggtcagg
     6241 gtgcaacggg ctattctggc ggtggatgaa atcgtgaacc cgcagaccgg cgaggcccgc
     6301 aaaacggtgg agctggaccg ggagcggctg tttgcgtgct ttaaattcat gatgatggac
     6361 tgagcttata cattcactca gagaggtttt tatgacaaat cgtgttgttg ccgtggggcg
     6421 tgaagccctg ttgatggtcg ctgtcatggc cgggacgctg gtcgttctgg ccggaaacct
     6481 gctgaaactg ctttggcgcg tactgccggt gcttctgctg ctctacgggg ttgtgcagtt
     6541 tctggcgagg aacccggaga tccgtgactg gctgtttctg catttcgggc agggaggcac
     6601 ggcggccatt cttgtcgggg gaatactcct ctgtatgcgc cgcctgatga aataatgccg
     6661 gacagcaccg gctcggccgg tgctgttatt cctccccgag atggtcccgc attgccagcc
     6721 agagcttttc tgcatcctca tgcagccgtt cgcacatgtc ggccggggta tccatatcca
     6781 gctcgttcag tttctcgagc aacagcagcg actggtcctt aatgaatttc gccatgttga
     6841 gggcggtttt ttcctgcttc gtcatgtttc tgtccttccg ggcacggtct gcgtctgtcg
     6901 ggtatactgt ctcaaaaata gcggggtgag acaatggcgt gggtgacggt gagacaggcg
     6961 acggaactga cggggaaagc ccggagttcc ctgtatcggg atatggcgaa aggccgcgta
     7021 tcataccgta cggaagctga tggtggtcgg gtgatggata ccagcgagct tatccgggtg
     7081 tacggtgagt taagacaggg tgagactcac gagcgggacg ggttgagact gccggatgag
     7141 actgaaaaaa cggccttcga cgcgttgatc gcggagataa aagcactcag ggaggaggtg
     7201 gccggactgc ggcaggaaat gcaggccatg agactgctgg aacacaaacc ggagacagca
     7261 gagacaccgg ccggaacacc gcgccggtgg tggcagtggg gaaaacgctg acgcgctgtg
     7321 cttgtccaac cggcgacggc cacgcaatga attgcgcccc cgtcaccggt tatcagcgaa
     7381 cggtatgggg attctggcgc tggccaacca cggccagaac ccggactttc ctggtctggt
     7441 catcaacatc atacaggaca tgctgtccca tcagactgat ccggcgaacg tcctcgccat
     7501 agcgtgagtc cacttcatag aggcgcggga agtcggccag tttgtccgcc gcagtgagaa
     7561 cgcgatcggt aacgttctgc gcatcccaca gggatgaatt ttccgcgata tagtcaaaca
     7621 gcgtcgttac atcctcgatg gctccctgtt tccattccac ctcatacggc atcgcgcagt
     7681 tcctgataac ggccatgcag gcgctggcgg agttgttccg tggtcatgcc cggacgcgtg
     7741 tcagcacggg cggcggttac tttggcgcga acctgatctt catactctgc ctgggccagt
     7801 tcttcgttag ccaggcattg caacaacgca gctttgtccg ggccactttc cggcagtatg
     7861 cggggcaggt tttcagccag ttcccgcagg aagttggcgt tttcgtagtg tgtagtgttg
     7921 ctcattgcgg agactcccgg attaatgggg tgatatgaag aagtttagca gggggcggtc
     7981 tgaaagaata tgctcgtgtc agtcaggctg atgaagacga aagctggtaa aaaagggctt
     8041 acggcaccca ttttacgtca aactgcacgc catgcagttg cgtgcatagg catgcattag
     8101 gataaagttt accgggcgcg tttccggcgg ttttccgggt ggtttgttgc ctgttttatc
     8161 ccgtagccgc cggaaatgcc ctcagaccgt ctgagcggtg cgcgtaatgc gacgttatgg
     8221 taaatacccc ttaccggcgg cgctgtggct gccaggtgaa ggtggtttcc gtagcctcgt
     8281 atttctcccg acgcatttcc tgcgcccgcc tggcttcacg ttcgtcctga atggcacgaa
     8341 gcaccctttc ccgcacctgt tgttttaccg cctttggcag cgttttgttc cctttcaggc
     8401 ccatggccag tgcctgagcg acaaaggcgt ccgccaggcg cttttcttca gcgggagtca
     8461 gtttcaggtg cgtcatagtg gcctccgggc gtctcaggtt tcccgaacag tttaaccgca
     8521 ggtcctctcc tcatgccagt tagctttcag atgtgtctat accatctgaa atgtaagtgt
     8581 gatgaatgtc ttaaataata aaatttgttg ccatgagaaa ttttcgggaa aagacgcgat
     8641 ctgcttacag gtggctccgg gcgctttttt gtgcctgaag atgagaacta atctcatata
     8701 ttgacacctg aaaacgggag gagtaaggta ataattatac tgtgtatatg tacagtatat
     8761 tggctttaat ttattgtttt aaatgtcaaa gaggaaaacg at
//
