LOCUS       NZ_CP097078             8802 bp    DNA     circular CON 21-FEB-2024
DEFINITION  Klebsiella pneumoniae strain KP133 plasmid pKP133-2, complete
            sequence.
ACCESSION   NZ_CP097078
VERSION     NZ_CP097078.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28016300
            Assembly: GCF_027558775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Tian,D.
  TITLE     Evolution and transfer of virulence plasmids of hypervirulent and
            carbapenem resistant Klebsiella pneumoniae strains in hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Tian,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2022) Clinical Laboratory, Huashan Hospital, Fudan
            University, No. 12,Wulumuqi Road, Shanghai 200040, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 287x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/21/2024 03:20:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,385
            CDSs (total)                      :: 5,262
            Genes (coding)                    :: 5,127
            CDSs (with protein)               :: 5,127
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 60 of 135
            Pseudo Genes (incomplete)         :: 80 of 135
            Pseudo Genes (internal stop)      :: 18 of 135
            Pseudo Genes (multiple problems)  :: 22 of 135
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097078.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP133"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP133-2"
                     /geo_loc_name="China: Shanghai"
                     /lat_lon="31.23 N 121.45 E"
                     /collection_date="2017-12-06"
                     /collected_by="Dongxing Tian"
     gene            1..258
                     /locus_tag="M3I84_RS26840"
                     /old_locus_tag="M3I84_26810"
     CDS             1..258
                     /locus_tag="M3I84_RS26840"
                     /old_locus_tag="M3I84_26810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            343..492
                     /locus_tag="M3I84_RS26845"
                     /old_locus_tag="M3I84_26815"
     CDS             343..492
                     /locus_tag="M3I84_RS26845"
                     /old_locus_tag="M3I84_26815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            1976..3937
                     /locus_tag="M3I84_RS26850"
                     /old_locus_tag="M3I84_26820"
     CDS             1976..3937
                     /locus_tag="M3I84_RS26850"
                     /old_locus_tag="M3I84_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_032448356.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3937..4668
                     /gene="mobC"
                     /locus_tag="M3I84_RS26855"
                     /old_locus_tag="M3I84_26825"
     CDS             3937..4668
                     /gene="mobC"
                     /locus_tag="M3I84_RS26855"
                     /old_locus_tag="M3I84_26825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_094313769.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVAQRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMMMD"
                     /name="mobC"
     gene            4697..4960
                     /locus_tag="M3I84_RS26860"
                     /old_locus_tag="M3I84_26830"
     CDS             4697..4960
                     /locus_tag="M3I84_RS26860"
                     /old_locus_tag="M3I84_26830"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094313768.1"
                     /translation="MTNRVVAVGREALLMVAVMAGTLVVLAGNLLKLLWRVLPVLLLLY
                     GVVQFLARNPEIRDWLFLHFGQGGTAAILVGGILLCMRRLMK"
                     /name="hypothetical protein"
     gene            complement(4991..5170)
                     /locus_tag="M3I84_RS26865"
                     /old_locus_tag="M3I84_26835"
     CDS             complement(4991..5170)
                     /locus_tag="M3I84_RS26865"
                     /old_locus_tag="M3I84_26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_094313767.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     EKLWLAMRDHLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5239..5616
                     /locus_tag="M3I84_RS26870"
                     /old_locus_tag="M3I84_26840"
     CDS             5239..5616
                     /locus_tag="M3I84_RS26870"
                     /old_locus_tag="M3I84_26840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_094313766.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVMDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTAFDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTPRRWWQWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(5678..5977)
                     /locus_tag="M3I84_RS26875"
                     /old_locus_tag="M3I84_26845"
     CDS             complement(5678..5977)
                     /locus_tag="M3I84_RS26875"
                     /old_locus_tag="M3I84_26845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_094313765.1"
                     /translation="MPYEVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLTAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5967..6230)
                     /locus_tag="M3I84_RS26880"
                     /old_locus_tag="M3I84_26850"
     CDS             complement(5967..6230)
                     /locus_tag="M3I84_RS26880"
                     /old_locus_tag="M3I84_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_094313764.1"
                     /translation="MSNTTHYENANFLRELAENLPRILPESGPDKAALLQCLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(6536..6781)
                     /locus_tag="M3I84_RS26885"
                     /old_locus_tag="M3I84_26855"
     CDS             complement(6536..6781)
                     /locus_tag="M3I84_RS26885"
                     /old_locus_tag="M3I84_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7108..8793
                     /locus_tag="M3I84_RS26890"
                     /old_locus_tag="M3I84_26860"
     CDS             7108..8793
                     /locus_tag="M3I84_RS26890"
                     /old_locus_tag="M3I84_26860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_266051493.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQDADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             2108..8802
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(596..1174)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3938..4665
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.004"
                     /coverage="100.0"
                     /evalue="1.97e-140"
ORIGIN
        1 atggggctta aattacgttt gacatggttt gataagaaaa ctgaagagtt taaaggggaa
       61 gagtattcta aagactttgg tgacgatggt tctgtcattg aaagcttggg aatgccttta
      121 aaggataata ttaataatgg tgagtttgat ttgaagaaag aatgggttcc tttactacag
      181 ccatttttta aaaatcaaat aaaacctgat gtggataatt gttatatagc ttttgattat
      241 cgggacggta actggtaaca aaaaattatc aaggatgatt tgaaatactg acttccggtg
      301 ttcagggatg aatgccggag tctttaaata cagggaaagt atatgaaaaa ggtaaagact
      361 atttttcttt ttatattgat tgttgctggc tttcttctgg ccgcatgtca ggcaaattat
      421 atccgggatg ttcagggcgg aacggtggca ccatcgtcct cctctgaact gacggggatc
      481 gcggttcagt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      541 tttgccctgt aaacgaaaaa accacctggg gaggtggttt gatcgaaggt taagtcagtt
      601 ggggaactgc ttaaccgtgg taactggctt tcgcagagca cagcaaccaa atctgtcctt
      661 ccagtgtagc cggactttgg cgcacacttc aagagcaacc gcgtgtttag ctaaacaaat
      721 cctctgcgaa ctcccagtta ccaatggctg ctgccagtgg cgttttgcgt gctttcccgg
      781 gttggactca agtgaacagt taccggataa ggcgcagcag tcgggctgaa cggggagttc
      841 ttgcttacag cccagcttgg agcgaacgac ctacaccgag ccgagatacc agtgtgtgag
      901 ctatgagaaa gcgccacact tcccgcaagg gagaacggcg gacaggtatc cggtaaacgg
      961 cagggtcgga acaggagagc gcaagaggga gcgacctacc ggaaacggtg gggatcttta
     1021 agtcctgtcg ggtttcgccc gtactgtcag attcatggtt gagcctcacg gctcccacag
     1081 atgcaccgaa aaagcgtctg tttatgtgaa ctctggcagg agggcggagc ctatggaaaa
     1141 acgccaccgg cgcggccctg ccgttttgcc tcacatgtta gtcccctgct tatccacgga
     1201 atctgtgggt aactttgtat gtgtccgcag cgcccgccgc agtctcacgc ccggagcgta
     1261 gcgaccgagt gagcgaggag gcgcaatttc cccggcacct gcaagacggt caccggacgc
     1321 gctgatgtgg gatattgtcc cctgattaag tctttctatt ccctgacgat caattatcag
     1381 gataggtgaa ccatgtcggt ttttcgctga acattttacg ttaacctatc tctgttgctt
     1441 ttttctcctt gcggagaaat gcaagtcatc cccgctgggg cgtggctgac ttcaggggaa
     1501 aagaccttgc ttacgctggc tggacgccac cgacgcacgg tcttttcccc tctttctggc
     1561 tctgtccggc tggacgccgg ccacgccaga aattcacccg gcccccgtca ggctcacgcc
     1621 tgaccaccca caaaaatcca cggtgctgga cgcacctgac gaagcagcaa agctgctcct
     1681 gattttgcgg gtggtcagcc gctcgctatg gcgaactctg acgggggccg gaacaccgga
     1741 aaagaccgca ctggggtgcc gccttttcct ctcacacgct ggggcgtgct cgttgttcct
     1801 ccggggctgc gccttttccg catggggcgg aaagaggctg gcctccgcgg tgaaagctga
     1861 acgcaatcac cacggacgtg accgccgccg ccatgccttg gggcatgtcc gcggcttgtg
     1921 cagcgggggc tgcgcatcac gccggtaaac ccgtcgtctg aaggagagag attgcatgtt
     1981 taatacggat tcgcttgcct ggcagtggca tatcccggag ctggcccggc agatttaccc
     2041 gtgggtcagc tggctgtacc gggagtatta cagtctggcc ggtttttttc tggttatcgg
     2101 cctgtttttc ggtctggcag aactctgggc cgggcggatg cgattcgtgg ggctgagtca
     2161 taaggagcgg cgggagtatg ccgatcgcta taagcgcgat aataaccgga ctgcgccatt
     2221 ttcggtgctg gcctcgctgg attcgcccgg gcggatggtc agggcacggc gacatgtctg
     2281 gaactacgca tactggctga ccggcgctgc cggtctggcc cttctggggg ggctggttgt
     2341 cgccgtgttt atcgagagct ggggagaaac cgtcctgcgt ccggacctct acgcgaacgc
     2401 agattattac ctgctgcagc cggctaacct gttcgggatt gcctggtgtg ccggaggtct
     2461 tgtcgcaggc gtcctcctgg cgcggctgct ggcgttcctg ctgcttgacg ggcattttgc
     2521 ggccgctgac gaggcggtga acgcgaaact gaatcaggag agccggcgca gcagccagcg
     2581 gaccggagaa atgaccgacg tccgccacct gcatttcggg gagccggtac cggttaacgc
     2641 gctggccgat ttttccacgg agcaggcccg gaaacagcag gcggtgtttc tgggtaagga
     2701 tgagcagggc cagccggtgc tggtcccgcg tgatacctgg cgcaagacca acattcagat
     2761 cctcggcctg cccgggagcg gcaagagcgt gatggccacc aacgcgctca ttcgctgcgt
     2821 acgcgatttc ggggacgcgg tggtgtactt cgacccgaaa ggggacgcgt gggcgccgca
     2881 cgtcttccgg gcgcactgcc cggacttcac cctgctggat ctgcggccgg ggaaaccggc
     2941 gcagctgaat ctgttccgcg atctcgacca gtacgccctg aagaatcttc tggttgccgg
     3001 atttaacctg agtgagaccg gcgacgtggc ggaccactac cgcatcagcg agcagaaagc
     3061 ggcgaagctg attgcggagc agtttccgca gggggcaaat attcagcagg tgctggcggc
     3121 ggcgtacgca ctgccggagg ccctgaaaaa ggacgtgaag gggctgatca ccaagctgga
     3181 gaacgtggcc gacctgagcg tccttcagac ggacagcggc atcgacgtgg cggggataat
     3241 caacggcggc ggctgcctgt acgtcatcgg ctcgatggac gatgaggcgg tgatccgggt
     3301 gcagaagatg ctgtttgccc gctgcgcgca aatcatcatt gcgcgggacg agttccggag
     3361 gtggccgcac gcgagcatca tgctcgatga gattaaatat ctgctgtcga agtacgtgct
     3421 gaacgcgctg gggacgctgc gcagccggga ctgcaacctg ctgctcgcgc accagtcgct
     3481 gggcgacttc ggccagtgcg ggcaggatct gcccgctgat tttgtgaaaa ccacggtact
     3541 ggataacacg ccgatccgct ggttctaccg ggcggccagc caggagtccg ctcagtgggc
     3601 ggccggacag accggggaga tccgggtgga cgtggagcgc cgccgggcca gccgggaggc
     3661 ggggaacgtg gagcatatca gcggggacag cttcatccag aaggaggcgc ggccgctgtt
     3721 tgacgtgaac accctgcaac atctgcctga cgggttcgcg gtgatgaccg ggctgggcgt
     3781 ggcgcggctg gcgttcagca gcccgctgcg tgttgatcgc cgggaaattc cgctgaaaac
     3841 cttccctgtg ctggcgaaaa cggacccgct ggcggagtac cagtcggagg ccgggcggca
     3901 gcggccgggt gatgatgatt ttgcggggct gtactgatgg cactggagcg atacaacgtc
     3961 agccacgcga agcggcaggc acggaacgcg gagaaaacac ggctgaccct gcgctggctg
     4021 cgggaagaac tgtgttccac ggcggagctg gttgcgcaac ggctggggat tgcggcggta
     4081 cagccggttt accgcttcct ggacagcctg gtggcgaaag ggctgctggt ccgggcgaaa
     4141 tacccggttg acggccgtca ggtcagcgtg tgggggctga cgccgcacgg ggtggcgttc
     4201 tcgttcgacg aggacgagcc gctgacggac attattccgt ttcagccgtc gcgggtgtcg
     4261 gcggcgcagc tgccgcaccg gctggccgtg cagtccctgc gcctcgccat ggaggcccgg
     4321 ggggccaccg gctggcggta cctgcaccgt attgccctga aagggatgaa ggtgccggac
     4381 gcgctggcgg agctggacgg cagaacggtg gcgtttgagg tggaacgcac cgtgaagagc
     4441 cgcaggcgct atcaggaggt ggtgtccggg tatctgttta accgccgggc gaacggcatc
     4501 gatgaaatct ggtacatttg cccggaccgg gcgacgcagg tcagggtgca acgggctatt
     4561 ctggcggtgg atgaaatcgt gaacccgcag accggcgagg cccgcaaaac ggtggagctg
     4621 gaccgggagc ggctgtttgc gtgctttaaa ttcatgatga tggactgagc ttatacattc
     4681 actcagagag gtttttatga caaatcgtgt tgttgccgtg gggcgtgaag ccctgttgat
     4741 ggtcgctgtc atggccggga cgctggtcgt tctggccgga aacctgctga aactgctttg
     4801 gcgcgtactg ccggtgcttc tgctgctcta cggggttgtg cagtttctgg cgaggaaccc
     4861 ggagatccgt gactggctgt ttctgcattt cgggcaggga ggcacggcgg ccattcttgt
     4921 cgggggaata ctcctctgta tgcgccgcct gatgaaataa tgccggacag caccggctcg
     4981 gccggtgctg ttattcctcc ccgagatggt cccgcattgc cagccagagc ttttctgcat
     5041 cctcatgcag ccgttcgcac atgtcggccg gggtatccat atccagctcg ttcagtttct
     5101 cgagcaacag cagcgactgg tccttaatga atttcgccat gttgagggcg gttttttcct
     5161 gcttcgtcat gtttctgtcc ttccgggcac ggtctgcgtc tgtcgggtat actgtctcaa
     5221 aaatagcggg gtgagacaat ggcgtgggtg acggtgagac aggcgacgga actgacgggg
     5281 aaagcccgga gttccctgta tcgggatatg gcgaaaggcc gcgtatcata ccgtacggaa
     5341 gctgatggtg gtcgggtgat ggataccagc gagcttatcc gggtgtacgg tgagttaaga
     5401 cagggtgaga ctcacgagcg ggacgggttg agactgccgg atgagactga aaaaacggcc
     5461 ttcgacgcgt tgatcgcgga gataaaagca ctcagggagg aggtggccgg actgcggcag
     5521 gaaatgcagg ccatgagact gctggaacac aaaccggaga cagcagagac accggccgga
     5581 acaccgcgcc ggtggtggca gtggggaaaa cgctgacgcg ctgtgcttgt ccaaccggcg
     5641 acggccacgc aatgaattgc gcccccgtca ccggttatca gcgaacggta tggggattct
     5701 ggcgctggcc aaccacggcc agaacccgga ctttcctggt ctggtcatca acatcataca
     5761 ggacatgctg tcccatcaga ctgatccggc gaacgtcctc gccatagcgt gagtccactt
     5821 catagaggcg cgggaagtcg gccagtttgt ccgccgcagt gagaacgcga tcggtaacgt
     5881 tctgcgcatc ccacagggat gaattttccg cgatatagtc aaacagcgtc gttacatcct
     5941 cgatggctcc ctgtttccat tccacctcat acggcatcgc gcagttcctg ataacggcca
     6001 tgcaggcgct ggcggagttg ttccgtggtc atgcccggac gcgtgtcagc acgggcggcg
     6061 gttactttgg cgcgaacctg atcttcatac tctgcctggg ccagttcttc gttagccagg
     6121 cattgcaaca acgcagcttt gtccgggcca ctttccggca gtatgcgggg caggttttca
     6181 gccagttccc gcaggaagtt ggcgttttcg tagtgtgtag tgttgctcat tgcggagact
     6241 cccggattaa tggggtgata tgaagaagtt tagcaggggg cggtctgaaa gaatatgctc
     6301 gtgtcagtca ggctgatgaa gacgaaagct ggtaaaaaag ggcttacggc acccatttta
     6361 cgtcaaactg cacgccatgc agttgcgtgc ataggcatgc attaggataa agtttaccgg
     6421 gcgcgtttcc ggcggttttc cgggtggttt gttgcctgtt ttatcccgta gccgccggaa
     6481 atgccctcag accgtctgag cggtgcgcgt aatgcgacgt tatggtaaat accccttacc
     6541 ggcggcgctg tggctgccag gtgaaggtgg tttccgtagc ctcgtatttc tcccgacgca
     6601 tttcctgcgc ccgcctggct tcacgttcgt cctgaatggc acgaagcacc ctttcccgca
     6661 cctgttgttt taccgccttt ggcagcgttt tgttcccttt caggcccatg gccagtgcct
     6721 gagcgacaaa ggcgtccgcc aggcgctttt cttcagcggg agtcagtttc aggtgcgtca
     6781 tagtggcctc cgggcgtctc aggtttcccg aacagtttaa ccgcaggtcc tctcctcatg
     6841 ccagttagct ttcagatgtg tctataccat ctgaaatgta agtgtgatga atgtcttaaa
     6901 taataaaatt tgttgccatg agaaattttc gggaaaagac gcgatctgct tacaggtggc
     6961 tccgggcgct tttttgtgcc tgaagatgag aactaatctc atatattgac acctgaaaac
     7021 gggaggagta aggtaataat tatactgtgt atatgtacag tatattggct ttaatttatt
     7081 gttttaaatg tcaaagagga aaacgatatg agtggtggag acggtcgagg tccgggtaat
     7141 tcaggtctgg gacataatgg tggtcaggcc agtgggaatg tgaacggtac gtctggtaaa
     7201 ggtggccctt catcaggtgg tggtacggat ccaaacagcg ggccgggctg gggtacgacg
     7261 catacgccga atggagatat tcataactat aatccggggg agtttggtaa cggcgggagt
     7321 aaacccggtg gtaatggtgg taacagcggc aatcatagcg gtagctctgg tggcggacag
     7381 tcttcggcca ctgcgatggc cttcggtctg cctgccctgg ctactccggg ggctgaagga
     7441 ctggctttat cagtttccgg cgatgcgttg tcggccgccg ttgctgatgt gctggctgcc
     7501 ctgaaggggc cgtttaagtt tggtctgtgg gggattgcga tctacggtgt gctgccttct
     7561 gagattgcaa aagatgatcc gaaaatgatg tcaaaaatta tgacgtcatt accggccgat
     7621 gcggtgacgg agactccggc aagtacttta ccactggacc aggcgacggt tcgtgtcaga
     7681 caacgggttg tggatgtggt gaaggatgag cggcagcata ttgcggttgt cgcaggtcgg
     7741 ccaatgagtg tccctgtggt ggatgcgaaa ccgacaaaac gtccgggggt attcagtgtg
     7801 tcgattccgg gtctcccgtc tctgcaggtg agcgtaccga aaggtgttcc gacagcgaaa
     7861 gccccgccaa aaggcattgt tgctgaaaaa ggtgattcac gtccggctgg ttttacagcc
     7921 ggtggtaact cccgtgaggc cgttattcgt ttcccgaaag agaccggaca gaagccggtt
     7981 tatgtgtcgg tgacagatgt tcttaccccg gcacaggtaa aacagcgtca ggaggaagaa
     8041 aagcgtcgcc agcaggcatg ggacgccgct catccggaag aggggctgaa aagagactat
     8101 gataaagcga aagctgagct ggatgccgaa gataaaaata ttgcgacctt aaacagccgc
     8161 attgcatcga cagagaaggc gctccccggt gcaagggctg ctgttcagga cgccgataaa
     8221 aaggtaaaag aggcagaggc aaataaagat gattttgtga cttataaccc gcctcatgaa
     8281 tatggctccg ggtggcagga tcaggttcgc tatcttgata aggatattca gaatcagaat
     8341 gagaaattaa aagcggctca ggcatcttta aacgcaatga atgaatcctt atccagagat
     8401 aaggctgcac ttaccggggc gatggagagc cggaaacaaa aggagaaaaa agcgaaggat
     8461 gcagaaaata agttaaatga ggaaaagaac aagcctcgca aggggactaa agactacggc
     8521 catgattatc atccagtacc taaaacagaa gacataaagg gacttggaga gttaaagcga
     8581 ggtgatccta aaacacctaa acaaggtggt ggtggtaagc gggcccgatg gtatggcgat
     8641 aaaaaacgta aaatttatga atgggattcc cagcacggtg agcttgaagg gtatcgtgcc
     8701 agtgatggcg aacacctagg cgcatttgat cccaaaacgg gtaagcaggt taaagggcca
     8761 gatccaaaac ggaacattaa aaaatatctt tgagaggtaa at
//
