LOCUS       NZ_CP097026            18501 bp    DNA     circular CON 19-MAY-2024
DEFINITION  Enterococcus faecium strain AT45b plasmid pAT45b-d, complete
            sequence.
ACCESSION   NZ_CP097026
VERSION     NZ_CP097026.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28025462
            Assembly: GCF_023299725.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18501)
  AUTHORS   Biggel,M.
  TITLE     Florfenicol-resistant Enterococci from pet food
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18501)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2022) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023299725.1-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 04:48:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,683
            CDSs (with protein)               :: 2,683
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 43 of 123
            Pseudo Genes (incomplete)         :: 75 of 123
            Pseudo Genes (internal stop)      :: 32 of 123
            Pseudo Genes (multiple problems)  :: 21 of 123
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP097026.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..18501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AT45b"
                     /isolation_source="pet food (BARF)"
                     /db_xref="taxon:1352"
                     /plasmid="pAT45b-d"
                     /collection_date="2022"
                     /collected_by="ILS, University of Zurich"
     gene            complement(89..301)
                     /locus_tag="M2917_RS14305"
                     /old_locus_tag="M2917_14305"
     CDS             complement(89..301)
                     /locus_tag="M2917_RS14305"
                     /old_locus_tag="M2917_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002324171.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIEY
                     RTEYLELTNQTDFLLIRKQQEQEKN"
                     /name="hypothetical protein"
     gene            complement(322..1173)
                     /locus_tag="M2917_RS14310"
                     /old_locus_tag="M2917_14310"
     CDS             complement(322..1173)
                     /locus_tag="M2917_RS14310"
                     /old_locus_tag="M2917_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002287227.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILDL
                     TVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAGKL
                     FKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILFFVG
                     YVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKGIRSI
                     LKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDLELPMK
                     DK"
                     /name="hypothetical protein"
     gene            complement(1274..1570)
                     /locus_tag="M2917_RS14315"
                     /old_locus_tag="M2917_14315"
     CDS             complement(1274..1570)
                     /locus_tag="M2917_RS14315"
                     /old_locus_tag="M2917_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication control protein PrgN"
                     /protein_id="WP_000053907.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTRE
                     KTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
                     /name="replication control protein PrgN"
     gene            complement(1705..3198)
                     /locus_tag="M2917_RS14320"
                     /old_locus_tag="M2917_14320"
     CDS             complement(1705..3198)
                     /locus_tag="M2917_RS14320"
                     /old_locus_tag="M2917_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_000947691.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSKQ
                     LMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINTFV
                     VDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVIDVA
                     KKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSMKLES
                     DQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTLSLAYY
                     SSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELCQQWVSP
                     TLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLNLSKKELA
                     SQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLSKEKRLDYS
                     TRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
                     /name="primase C-terminal domain-containing protein"
     gene            3810..4763
                     /locus_tag="M2917_RS14325"
                     /old_locus_tag="M2917_14325"
     CDS             3810..4763
                     /locus_tag="M2917_RS14325"
                     /old_locus_tag="M2917_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000429439.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLFE
                     KDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGSPG
                     SEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAADYII
                     AMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQKTID
                     KYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYYEQHGD
                     IEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
                     /name="ParA family protein"
     gene            4735..5010
                     /gene="prgO"
                     /locus_tag="M2917_RS14330"
                     /old_locus_tag="M2917_14330"
     CDS             4735..5010
                     /gene="prgO"
                     /locus_tag="M2917_RS14330"
                     /old_locus_tag="M2917_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="WP_001196543.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVDP
                     PVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
                     /name="prgO"
     gene            5238..5918
                     /locus_tag="M2917_RS14335"
                     /old_locus_tag="M2917_14335"
     CDS             5238..5918
                     /locus_tag="M2917_RS14335"
                     /old_locus_tag="M2917_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            6046..7005
                     /locus_tag="M2917_RS14340"
                     /old_locus_tag="M2917_14340"
     CDS             6046..7005
                     /locus_tag="M2917_RS14340"
                     /old_locus_tag="M2917_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="WP_000222577.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINFL
                     KEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPISCS
                     VRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEFGHI
                     EGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFKSITF
                     DCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQVDQAF
                     ISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
                     /name="IS30-like element IS1252 family transposase"
     gene            complement(7438..8010)
                     /locus_tag="M2917_RS14345"
                     /old_locus_tag="M2917_14345"
     CDS             complement(7438..8010)
                     /locus_tag="M2917_RS14345"
                     /old_locus_tag="M2917_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000170424.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRDR
                     PKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTSMG
                     RMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELLTSG
                     KSYKEVEAITGFSRSTLFRIKKKIEES"
                     /name="recombinase family protein"
     gene            complement(8026..8631)
                     /locus_tag="M2917_RS14350"
                     /old_locus_tag="M2917_14350"
     CDS             complement(8026..8631)
                     /locus_tag="M2917_RS14350"
                     /old_locus_tag="M2917_14350"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="WP_000599739.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTFK
                     GLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEIIKK
                     YVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDLEIR
                     YLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
                     /name="protein adenylyltransferase Fic"
     gene            complement(8829..9509)
                     /locus_tag="M2917_RS14355"
                     /old_locus_tag="M2917_14355"
     CDS             complement(8829..9509)
                     /locus_tag="M2917_RS14355"
                     /old_locus_tag="M2917_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            <9541..10254
                     /locus_tag="M2917_RS14360"
                     /old_locus_tag="M2917_14360"
                     /pseudo=""
     CDS             <9541..10254
                     /locus_tag="M2917_RS14360"
                     /old_locus_tag="M2917_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /name="C40 family peptidase"
     gene            10251..11183
                     /locus_tag="M2917_RS14365"
                     /old_locus_tag="M2917_14365"
     CDS             10251..11183
                     /locus_tag="M2917_RS14365"
                     /old_locus_tag="M2917_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="WP_001224319.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVLL
                     GLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSIDN
                     RMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQLITE
                     GENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSITTNEI
                     NEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTVSLTVE
                     YIDQQTKATQVSQFDLVLEKNGSNWKIVK"
                     /name="conjugal transfer protein"
     gene            11428..11544
                     /locus_tag="M2917_RS14370"
                     /old_locus_tag="M2917_14370"
     CDS             11428..11544
                     /locus_tag="M2917_RS14370"
                     /old_locus_tag="M2917_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="WP_001791010.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
                     /name="tetracycline resistance determinant leader peptide"
     gene            11560..13479
                     /gene="tet(M)"
                     /locus_tag="M2917_RS14375"
                     /old_locus_tag="M2917_14375"
     ARG             11561..13476
                     /name="tet(M)"
                     /gene="tet(M)"
                     /gene_name="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            13673..15049
                     /gene="tet(L)"
                     /locus_tag="M2917_RS14380"
                     /old_locus_tag="M2917_14380"
     ARG             13674..15046
                     /name="tet(L)"
                     /gene="tet(L)"
                     /gene_name="tetracycline efflux MFS transporter Tet(L)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            15613..16875
                     /gene="mobV"
                     /locus_tag="M2917_RS14385"
                     /old_locus_tag="M2917_14385"
     CDS             15613..16875
                     /gene="mobV"
                     /locus_tag="M2917_RS14385"
                     /old_locus_tag="M2917_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000119405.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYD
                     LKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFE
                     ESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKF
                     PEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVK
                     SDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDND
                     RLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSK
                     ISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNE
                     FELTHNREVKKERSRDQGMSL"
                     /name="mobV"
     gene            17120..>17422
                     /locus_tag="M2917_RS14390"
                     /old_locus_tag="M2917_14390"
                     /pseudo=""
     CDS             17120..>17422
                     /locus_tag="M2917_RS14390"
                     /old_locus_tag="M2917_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            complement(17477..18157)
                     /locus_tag="M2917_RS14395"
                     /old_locus_tag="M2917_14395"
     CDS             complement(17477..18157)
                     /locus_tag="M2917_RS14395"
                     /old_locus_tag="M2917_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     ARG             13764..15049
                     /name="tet(45)"
                     /gene="tet(45)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="76.35"
                     /coverage="93.45"
     Typing          complement(1706..3198)
                     /name="rep_cluster_185"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          15614..16872
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.19"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttacaaata aaggctttta gagacgttct ggaattttag attccttgaa ttgttcccat
       61 tcatcaatca attggctata agtagccatt aatttttttc ttgctcttgt tgctttcgaa
      121 tcaacaaaaa atcagtttga ttcgttaatt ctaaatattc tgttcggtat tcaatgttca
      181 tcgtacccgt tttactacat agtaactgat tatgacgttc tttcactttg aataactgct
      241 ccttctttaa ggtttttaac tccatgccaa ctataagcac aatcctgtat agaggaatca
      301 ttttcaaacg attctccata attatttatc tttcattgga agttctaaat cactactgtt
      361 ccaaaaaatg tatggtttgt tttgacgaat attacttcca tctaaccaca attttccgat
      421 gccatatcgt aggcttgctt cgacttcata aaaattgtag atctcttttc ttcgatctat
      481 tgctttcatt tcctggccat tttttaaaat agatctgatg cccttaacag tagattcatg
      541 gatactcgtg tgaacgatat ccgtattgat caattcgtca tatcttccaa aatcaacatt
      601 gtactcataa ccaagaagtg tcggaatcag gtcaatttca atttcttttt caattgaacg
      661 tttaataagt ttcgtggata catacccgac aaaaaagaga ataataccca cttcaaacaa
      721 atacatgtgt ccatttacaa agtgaataaa cgtcaataaa aaatccttca ctccttttgt
      781 tgttcttcca gattgttcaa tttgttttat taaagtcgct agaattgcat ttacaacaat
      841 taaatcgaat aggcttttga aaagttttcc tgctgttaat ttttgcctgg taaattgcat
      901 gactaattga gtaaaaatag acaatagcat gacaaaaatc aacgtcatta gcactgtaaa
      961 aatgtctgat gctgcaatcc tattgggatc atttatcaag taataagccg ttccaaatac
     1021 tccaatcgag taaacggtta aatcaagaat aagtcgtgcc ccgtaactac aaagttttcc
     1081 caatggccat aattgcaata cccaaaattt ctttcgtttg ctatttcttt tcagaggaag
     1141 ccaggtgtgt tcaatccagt cgtccgattt catttcgatt tcttccctaa attctttcct
     1201 ttaacgtttt cctttgctac tttagcagcg ataggttgct caatgaacca attttttaag
     1261 taaataaaaa ctactactgg atatacttct tcctttttct taactcgtta gctattttat
     1321 actcaatata ctcctgctct aacacacaca aacaatcata tacctgatcc atagtctccc
     1381 cagaagctaa ggacaaccca tgaagaaact caatcggcaa agaagcaact gttttttccc
     1441 tggtaaccca agaagacaaa gtcccctgct taaaaccata aagctcacag aacacttcca
     1501 cagataatcc caaacgacca atgataaaca cattaacagg atgtgaatag acataatttt
     1561 ttaacgacat acaacttcct ccatgataca taagactgca tacgcagtta taagtatacc
     1621 acctacacgt agctaggcaa agggagcaac acctacagaa gtatcataga tatattgtat
     1681 agggcaattt gcacctttta tttttcaacc tgtatctact tcaaaaagat tcacttcttt
     1741 aagagggttc aagggggttg ataattgtct aaatgtctct tctactaagc tctcgttgag
     1801 tccaaatacc tctgtgatcc ttgttgaata atctagtctc ttttctttac ttagttgaat
     1861 aaccctcgcg aacaacgttt tgactgaagc aatgatcatt ccgccatttc ttcctcgtgt
     1921 taccttgtag aagactttat tctcctctcg taactcattt aagatcttat caagtgttcg
     1981 cttgggcatc gttagttgtg aagccaattc tttttttgac aggttcaaat aaggctcact
     2041 agcaagactt ttttcgctta gataagccaa tagatcttct ttccattcat ttttatgact
     2101 atattttctt tgatctcgtg gctttttaaa tttccaccag ccacgtcgtt gaatgaacag
     2161 atcattattt tttaaggttg gtgaaaccca ttgctggcac aattcaataa tgaattctct
     2221 gcttgctgct tgataattac cagaataagc actacgtaca atctttacta attctgcttc
     2281 agatagcggt tcctgtaacc gttcgttgaa tgtgaacata ttgtattcgc aagtatccaa
     2341 tgcataacct gaagagtaat aagctaaaga aagcgtgaaa atcacattat ttcgaccaag
     2401 acgtccttct ccgccgacga tgtttgcttt atgaagtaac atatcgaacc acggttcatc
     2461 tacttgacga atttcttttt tctcaggaaa gacaattagt tttcgttctc gatcttcttt
     2521 tgttttttga tctgattcaa gcttcatcga ccattgaatc cagtcagcaa aagaatactg
     2581 atagttcatt tctttaaaaa caatattatt ttcttgtggg aatcgagcaa ttccgaagtg
     2641 gttacatcct agatccactc cggacaattt ttcagccaac tgtagacgaa ttgtttttga
     2701 aatttttttt gctacatcga tcaccttgaa attgcttttc tttgtgacat agcctggtgt
     2761 ctttagtaca aaataaactt gataaccatg atctgattct aaaattagcg ttggcatgta
     2821 gcccacttga tctaaacagg ctaaaataat ttcgccttga tggttctctt ttgaatcgat
     2881 atcaactaca aatgtgttga tttgacttaa gtttttctca gaatgacctt taaccactgt
     2941 tcgataatca tccgcataag taccaaagcg ataggtatta ggtgtccaat gcgttagttt
     3001 cttttcgttc tctaaaatag cttcttccga agtaatgacc ataccacgcc caacattcat
     3061 taattgcttg ctacggtacg caaagatagc accactccta ctggaatcaa tatgacctgc
     3121 aggtttcatg gtgctgtttc gatatttgta tgtacgtaag ccgtttttaa gaatagctga
     3181 ataaatttcg ctcaacatcg attgtaacgt cctttctcaa acaaaaagag aacacagttt
     3241 agctttaatc tcagccatag tatgtgttct ctcttcagtg tttaagaata tttaatttaa
     3301 gtacactaaa taaactttct tctgttgagt tgctcgcctt tttttggtag aataaaggca
     3361 atcggaaaag gtatccgatc aacttttaat ataagtagtt ggtagctact tacatattct
     3421 tgagacgagc gtgaacacat gtcctaacca tgtgattcag aaataattga ttctgctaaa
     3481 atcaattatt tccgtctctt ttttttatgt aatttttctg tttaaagagt accactgctt
     3541 ttgctacttt gtaaatagtg aattatctgt ggtttctata gaattaagtt tacctctttt
     3601 cactctaaat tgtcaacgca aaacgcacaa atatattttg acattttgaa tatatctgat
     3661 atactgaata tagcaaatat atttagtata tccactatat tccttataaa gtctgaattc
     3721 ttatcaattt ggagtatatt agatatatcg aatatattaa gtatatgaag tatactaaat
     3781 atatttagta taggaaattg gagggaacta tggaaaactt acggactgca tttaaaacat
     3841 cagataaact aggaaaagtt gtaatgctta caggtagaaa aggcggcatt ggtaagacta
     3901 cagacaatga tttattagcg attgtttcta gtcaactttt tgaaaaagac gtgttgctaa
     3961 ttgattatga ccaacaaaga aatactactt ccaacattgg atcaacgtat caaattacta
     4021 gctttgatcg atccatgtct gcagcaatca aaaaagggga ctgggtttca ggtattactc
     4081 aagtaagtcc acatttatat attatggctg gctctcctgg atctgaagaa cttaatgaat
     4141 acttgtccga aaaatatcct gatcgtcgta agagatcatt ggccttcatt aaacctttag
     4201 aagaattgag aaaaaatttc gattatatct ttatcgactg cccaccatca acagataatg
     4261 tggttcgtgc attcttaact gctgcagatt atattattgc aatgcaagaa ttaaaacggt
     4321 acgcaatgga aggaacagaa gattttatta ataaagtttt agttccgatt gtcacgaatt
     4381 ttgaagagtc tcatcttcaa atcattggaa ttttgcctgt tcttttctca gtgcgacgtt
     4441 cctctcaaca cgcaaactat caaaaaacaa ttgataagta tggtgaatca aacattttta
     4501 aaagcattgt taaaggctcg gatcggttag aaatgtatgg tgagaatggt atccaattaa
     4561 atgattatgt agatcgaaga tggtgggcta tttttgcaga catctttaca gaattagaag
     4621 aaagaattga atactatgaa caacatggtg atattgaagg atttgattat acacctaaat
     4681 atgctgactc aatggccaat catatcctac ctcttggaaa ggagattaaa ctaaatggca
     4741 ttattaccaa cgaacaaaga taagaagaaa attggtgctg aagatatttc aattaaagat
     4801 gatattaatg tcgagttagg ggaacgaaag tacactgcga aagaaagaaa accagttcaa
     4861 gtagatcctc ccgtactaaa aaccattcgg gatcttagtt atgcaaaaga tattcctatg
     4921 tatgaaatag tcaaacttgc aatggacgcc tatgttgata ctttaaacga cgaggaacgg
     4981 attatatata atagacgatc aaaagattaa aatatagtaa gtatactaga tatagtaaat
     5041 atatttagta tacttactat atttttagct ttattaatcc taatccttca aaatatgtgt
     5101 attgtttggg ggtatacaac attcacaaat tttattgttt ctagttttta ctatactaaa
     5161 tatggttctg ttgcaaagtt ttaaatctac tatcaaataa ggtagaataa tagaaaaaga
     5221 tagcaggagg aatgacgatg aatcatttta aaggaaagca atttcagcag gatgtgatta
     5281 ttgtagccgt gggctactat cttcgttata accttagcta tcgtgaagtt caagaaatct
     5341 tatatgatcg tggcattaac gtttctcata cgacgattta tcgttgggtg caagaatatg
     5401 gcaaactact ctatcaaatt tggaaaaaga aaaataaaaa atccttttat tcatggaaaa
     5461 tggatgaaac gtacatcaaa attaaaggaa aatggcatta tttgtatcga gccatcgatg
     5521 cagatggttt aaccttggat atttggttac gtaaaaaacg ggacacacaa gcagcctatg
     5581 cttttcttaa gcggttagtg aagcagtttg atgaaccgaa ggttgtagtc acagataaag
     5641 ccccctctat tacaagtgcc tttaagaaac taaaagaata cggcttttat caagggacag
     5701 aacatcgtac cattaaatac ctgaataatt tgattgaaca agaccatcgt ccagtaaaga
     5761 gacgcaataa attctatcga agtttacgca ctgcctctac cacgattaaa ggcatggaag
     5821 ccattcgagg attatataag aaaacccgaa aagaaggcac tctcttcggg ttttcggtct
     5881 gtactgaaat caaggtatta ttgggaatcc cagcttaaat catagatacc gtaagggatt
     5941 ttattcttta cgccgattgt aaaattaagc tagacaaata aataagccat tcgtgctaca
     6001 ctcaaaatgt aattccgact aaagaaaaca taggagtgat cacgaatgac ctacacccat
     6061 cttaccacgg atgaactggt gatgatagag tcttactacc accaaaacat caaaattgtg
     6121 aaaattgctg agtacctgaa acgatctcga acaccgattt ataacgtcat taatttcttg
     6181 aaagaaggcc atacggccct cgaatactac caacagtata aagaaaataa gaaacggtgt
     6241 ggcagacgaa acatcgtctt gccaaagaaa caacagtcct acattaaaga aaaaattgcc
     6301 caaggctgga cgccagacgt tatcattggg cgtaaagaga aaccaatcag ctgttccgta
     6361 agaacccttt atcgccagtt caaggaaaag gtgttcgatg aatcgacttt gcccatgaag
     6421 ggtaagcgga aaccaaatgg acatcaagaa cgccgaggaa aacaggcttt taaacggaat
     6481 atcgttgaaa gagaaactga ttacccgaca tttaaagaag agttcggcca tatcgaaggc
     6541 gataccattg taggcattca tcataagagt gctgtcataa ctttggtaga acgtctatcg
     6601 aaaatcatca tcaccttgaa gcccgagggg cgcaaggcga tcgatattga gaatactctg
     6661 aaccactggt ttggatcgat tcccagacat ttatttaaat ccattacctt tgattgtggg
     6721 aaagaattct ctaattggaa ggctttaagt aaccaacatg acatcgctat ttatttcgct
     6781 gatccaggga ctccttcgca acgagcactg aatgaaaatt ccaatggctt gctgcgaaaa
     6841 gatggtctgc caaaaaacat ggattttaat caagtcgacc aagcttttat ctcttcagtg
     6901 gcgaataaga gaaacaatat tccacgaaag tccttggatt atcaaacacc cctggaagta
     6961 tttatgagtt acatgaatga agatattttg tctagcttaa tttgacaaat caaattattt
     7021 aaaactttgc aacagaacct tagcagcagt gctgattttt atgatagtta tttggttctt
     7081 aaatgaaaga tctaaagaca atcaacaccg tagggatatg gagataaaat caatagagtc
     7141 aatagattat ttatcgataa taacactaaa aaagccacgt tcctaaacgt gacttacgct
     7201 gaacgatatg tgttgtttgg tagacgaaca aatcgttcat tataaatata tgatggtatc
     7261 ctaatatagt aagcgtggct agcatacctt taattacttt accttttaca gttaccatac
     7321 acattttttg agcagaaatc aagtaaataa aaaaatatta tatttcacaa catgctaata
     7381 gactcatatc tagaacgcat taagtattta cctgtctggt tcgctgagac ttctttttta
     7441 actttcttca attttcttct ttattctaaa aagcgtactg cgactgaaac cggttatagc
     7501 ttcaacttcc ttatacgatt tacctgaagt aagcagttca tatgcgtgcc tcttcttcgg
     7561 tgtgattgtt gctttcggac gtccttctct gaaattagga ttattctttc ttgcaaacat
     7621 ttttccttct tgggtacgtt ccacaatcat atcacgttcc atttctgcta cactcaacaa
     7681 cgtccgtgtc accattcgcc ccatggacgt attttcaatc gttcctagat tcaaaacctt
     7741 gatcgttacg ttatcatcga gtaaaggttc aatgatctct aatgcttctc tcgtgttacg
     7801 agcaaatcga tcaagcttcg taacaactat ttcatctcca ggcttcagac cagccaacag
     7861 ctgattgaat tttggccgat cccgttttgt acccgtaaat ttttctgaaa aaatattctt
     7921 cttctctacg ccgttctcaa gcaactgttg aatttgttct tttgtacttt gagaagcggt
     7981 tgagacacgt gcatatccat atttcgtcat ttggtccacc tacttttaca aatcatctat
     8041 cgtatactca gaataccctt catagaaata gctgacatcg attcctttca tatataactc
     8101 gcgatcgtca attttatcag tcagagcatt ggaaattaga tagcgtattt ccaaatcgtt
     8161 tatgggactt cgctccattg cagaaagata gtctgcttta tcgattaagt tccaatctac
     8221 aactttttgc aattcttcct ttaaaatgag atccagccaa attctagtac ttcggccatt
     8281 tccttctcga aatgggtgag cgatattcat ctccacatat ttcttgataa tctcttcaaa
     8341 agaagtttgt ggcatctgat caatatgctg taacgaatgt tctaaataca taactggtgc
     8401 aaatcgaaaa tttccttttg caatattgac cgatcggatt tttccggcaa aatcgtaaat
     8461 atccgaaaat agaaattcat gaatttcaga taaaccttta aatgtaccaa cttcgatttc
     8521 atttattttt ccacaatcgt acaattcttt cgctttttgt ttactgagtt tttcttcaac
     8581 cttagccaac tcaacttgat tggttaatcc caatttattc tctaaaatca tgcgaaatct
     8641 ccttaacttt atgacataag tttatgaaac actctcaacc ccattatatc gcgattttgt
     8701 ttatgtgtca aaaactatta agtttctgac ccccgataga tttttcaagt gtctctattt
     8761 tgcacagtct agttggttct gttgcaaagt tttaaataaa gaataaaatc ccttacggta
     8821 tctatgattt aagctgggat tcccaataat accttgattt cagtacagac cgaaaacccg
     8881 aagagagtgc cttcttttcg ggttttctta tataatcctc gaatggcttc catgccttta
     8941 atcgtggtag aggcagtgcg taaacttcga tagaatttat tgcgtctctt tactggacga
     9001 tggtcttgtt caatcaaatt attcaggtat ttaatggtac gatgttctgt cccttgataa
     9061 aagccgtatt cttttagttt cttaaaggca cttgtaatag agggggcttt atctgtgact
     9121 acaaccttcg gttcatcaaa ctgcttcact aaccgcttaa gaaaagcata ggctgcttgt
     9181 gtgtcccgtt ttttacgtaa ccaaatatcc aaggttaaac catctgcatc gatggctcga
     9241 tacaaataat gccattttcc tttaattttg atgtacgttt catccatttt ccatgaataa
     9301 aaggattttt tatttttctt tttccaaatt tgatagagta gtttgccata ttcttgcacc
     9361 caacgataaa tcgtcgtatg agaaacgtta atgccacgat catataagat ttcttgaact
     9421 tcacgatagc taaggttata acgaagatag tagcccacgg ctacaataat cacatcctgc
     9481 tgaaattgct ttcctttaaa atgattcatc gtcattcctc ctgctatctt tttctattat
     9541 tctaccttat ttgatagtag atttaaaact ttgcaacaga accgtgtgaa gtatttcagt
     9601 gaattattag ccagtagcga aaggctcagt gtagatttag aatcggttat ccagtcctac
     9661 aattatggtg gtggtttctt agggtatgtg gctaatcgtg gaaataaata tacctttgaa
     9721 ctggctcaaa gtttctcaaa agagtattca ggtggcgaaa aagtgtctta ccccaatccc
     9781 atagccatac ctatcaatgg gggctggcga tacaactatg gcaatatgtt ttatgtgcaa
     9841 ctggtaacgc agtatcttgt cacaacagag tttgatgatg atacggtaca agccatcatg
     9901 gacgaagcac tgaaatatga gggctggcga tacgtttacg gtggagcttc cccgactact
     9961 tcttttgatt gtagcggact gacacaatgg acgtatggaa aagctggaat taacttacca
    10021 cgaaccgcac aacagcaata tgatgtgacc cagcatatcc cactatcgga agcacaagct
    10081 ggcgatttgg ttttctttca ttctacctat aacgctggct cttatattac tcatgttggg
    10141 atataccttg gcaataaccg tatgtttcat gcaggcgacc caatcggtta tgccgactta
    10201 acaagcccct actggcaaca gcatttagtg ggagcaggac gaatcaaaca atgagaaagg
    10261 aagatttaat gatgaaattt agaaaaaatc agaataaaga aaaacagata ccaaaggaaa
    10321 agaaacctcg tgtctataag gtcaatcctc ataaaaaggt tgtgattgcc ttgtgggtac
    10381 ttttagggct tagtttcagc tttgcgatat tcaagcactt tacagctata gatactcata
    10441 ctattcacga aacaactatc atagaaaagg aatacgttga tactcatcat gtagaaaatt
    10501 ttgtagagaa ctttgcgaaa gtctactatt catgggagca atccgataag tccattgata
    10561 atcgaatgga aagtctaaaa ggctatctga cagatgaact tcaagctctc aatgttgata
    10621 cagtacgcaa agatattcct gtatcgtctt ctgtaagagg atttcagata tggacggtag
    10681 agccaactgg cgacaatgag tttaatgtaa cctacagtgt agaccagctc attacagagg
    10741 gagaaaatac aaagaccgtc cactctgctt atatagtgag tgtctatgta gatggttctg
    10801 gaaatatggt actggttaag aatccgacca ttaccaacat acctaagaaa tcaagttata
    10861 aaccaaaagc cattgaaagt gaggggacgg ttgattccat tacaaccaat gaaatcaatg
    10921 agtttttaac gacgttcttc aagctctatc ctacagcgac agccagtgaa ctttcctact
    10981 atgtgaatga cgggatatta aaaccaatcg gaaaagagta catctttcaa gaactggtaa
    11041 atcctattca caatcgtaag gataatcaag tcacggtatc gctgacagtg gagtatatcg
    11101 accagcagac caaagcaacg caggtatctc aatttgattt ggtacttgaa aagaacggga
    11161 gtaattggaa gattgtaaaa taacaaatat tggtacatga ttacagatac tttgtaatca
    11221 tgtactcttt ttgataaaaa attggagatt cctttacaaa tatgctctta cgtgctatta
    11281 tttaagtgac tatttaaaag gagttaataa atatgcggca aggtattctt aaataaactg
    11341 tcaatttgat agcaggaaca aataattaga tgtccttttt taggagggct tagttttttg
    11401 tacccagttt aagaatacct ttatcatgtg attctaaagt atccagagaa tatctgtatg
    11461 ctttgtatac ctatggttat gcataaaaat cccagtgata aaagtattta tcactgggat
    11521 ttttatgccc ttttgggttt ttgaatggag gaaaatcaca tgaaaattat taatattgga
    11581 gttttagctc atgttgatgc aggaaaaact accttaacag aaagcttatt atataacagt
    11641 ggagcgatta cagaattagg aagcgtggac aaaggtacaa cgaggacgga taatacgctt
    11701 ttagaacgtc agagaggaat tacaattcag acaggaataa cctcttttca gtgggaaaat
    11761 acgaaggtga acatcataga cacgccagga catatggatt tcttagcaga agtatatcgt
    11821 tcattatcag ttttagatgg ggcaattcta ctgatttctg caaaagatgg cgtacaagca
    11881 caaactcgta tattatttca tgcacttagg aaaatgggga ttcccacaat cttttttatc
    11941 aataagattg accaaaatgg aattgattta tcaacggttt atcaggatat taaagagaaa
    12001 ctttctatgg aaattataat caaacagaaa gtagagctgc accctaatat gtgtgtgatg
    12061 agctgtacgg aacctgagca atgggatgtg gtaatagaag gaaatgatga ccttttagag
    12121 aaatatatgt ccggtaaatc attagaagca ttagaactcg aacaagagga aatcagaaga
    12181 tttcagaatt gctccttgta ccctgtttat catggaagcg caaaaagcaa catagggatt
    12241 gagcagctta tagaagtgat aacgaataaa ttttattcat caacatacag aaagaagtct
    12301 gaactttgcg gaaatgtctt caaaattgaa tattcggaag aaagacaacg tcttgcatat
    12361 gtacgccttt atggcggaat cctgcatttg cgggattcgg ttagaatatc ggaaaaggaa
    12421 aaaataaaaa ttacagaaat gtatacttca ataaatggtg aattatgtaa aattgataag
    12481 gcttattccg gggaaattgt tattttgcaa aatgagtttt tgaagctaaa tagtgttctt
    12541 ggagatacaa agctattgcc acagagagag agaattgaaa atccgctccc tctgctgcaa
    12601 acaactgttg aaccgagcaa acctcaacaa agggaaatgt tacttgatgc acttttagaa
    12661 atctccgaca gtgacccgct tctacaatat tatgtggatt ctacgacaca tgaaattata
    12721 ctttctttct tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat
    12781 catgtggaga tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat
    12841 gcagaatata ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta
    12901 tctgtatcac cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga
    12961 tacttaaatc aatcatttca aaatgcagtt atggaaggga tacgctatgg ttgcgaacaa
    13021 ggattatatg gttggaatgt gacggattgt aaaatctgtt ttaagtatgg cttatactat
    13081 agccctgtta gtaccccagc agattttcgg atgcttgctc ctattgtatt ggaacaagtc
    13141 ttaaaaaaag ctggaacaga attgttagag ccatatctta gttttaaaat ttatgcgcca
    13201 caggaatatc tttcacgagc atacaacgat gctcctaaat attgtgcgaa catcgtagac
    13261 actcaattga aaaataatga ggtcattctt agtggagaaa tccctgctcg gtgtattcaa
    13321 gaatatcgta gtgatttaac tttctttaca aatggacgta gtgtttgttt aacagagtta
    13381 aaagggtacc atgttactac cggtgaacct gtttgccagc cccgtcgtcc aaatagtcgg
    13441 atagataaag tacgatatat gttcaataaa ataacttagt gtattttatg ttgttatata
    13501 aatatggttt cttgttaaat aagatgaaat attttttaat aaagatttga attaaagtgt
    13561 aaaggaggaa ttaattatga agtgaaatga atgtaacagg gttcaattaa aagagggaag
    13621 cgtatcatta accctataaa ctacgtctgc cctcattatt ggagggtgaa atgtgaatac
    13681 atcctattca caatcgaatt tacgacacaa ccaaatttta atttggcttt gcattttatc
    13741 tttttttagc gtattaaatg aaatggtttt gaacgtctca ttacctgata ttgcaaatga
    13801 ttttaataaa ccacctgcga gtacaaactg ggtgaacaca gcctttatgt taaccttttc
    13861 cattggaaca gctgtatatg gaaagctatc tgatcaatta ggcatcaaaa ggttactcct
    13921 atttggaatt ataataaatt gtttcgggtc ggtaattggg tttgttggcc attctttctt
    13981 ttccttactt attatggctc gttttattca aggggctggt gcagctgcat ttccagcact
    14041 cgtaatggtt gtagttgcgc gctatattcc aaaggaaaat aggggtaaag catttggtct
    14101 tattggatcg atagtagcca tgggagaagg agtcggtcca gcgattggtg gaatgatagc
    14161 ccattatatt cattggtcct atcttctact cattcctatg ataacaatta tcactgttcc
    14221 gtttcttatg aaattattaa agaaagaagt aaggataaaa ggtcattttg atatcaaagg
    14281 aattatacta atgtctgtag gcattgtatt ttttatgttg tttacaacat catatagcat
    14341 ttcttttctt atcgttagcg tgctgtcatt cctgatattt gtaaaacata tcaggaaagt
    14401 aacagatcct tttgttgatc ccggattagg gaaaaatata ccttttatga ttggagttct
    14461 ttgtggggga attatatttg gaacagtagc agggtttgtc tctatggttc cttatatgat
    14521 gaaagatgtt caccagctaa gtactgccga aatcggaagt gtaattattt tccctggaac
    14581 aatgagtgtc attattttcg gctacattgg tgggatactt gttgatagaa gaggtccttt
    14641 atacgtgtta aacatcggag ttacatttct ttctgttagc tttttaactg cttcctttct
    14701 tttagaaaca acatcatggt tcatgacaat tataatcgta tttgttttag gtgggctttc
    14761 gttcaccaaa acagttatat caacaattgt ttcaagtagc ttgaaacagc aggaagctgg
    14821 tgctggaatg agtttgctta actttaccag ctttttatca gagggaacag gtattgcaat
    14881 tgtaggtggt ttattatcca tacccttact tgatcaaagg ttgttaccta tggaagttga
    14941 tcagtcaact tatctgtata gtaatttgtt attacttttt tcaggaatca ttgtcattag
    15001 ttggctggtt accttgaatg tatataaaca ttctcaaagg gatttctaaa tcgttaaggg
    15061 atcaactttg ggagagagtt caaaattgat ccttttttta taacaggaat tcaaatcttt
    15121 ttgttccatt aaagggcgcg attgctgaat aaaagatacg agagacctct cttgtatctt
    15181 ttttattttg agtggttttg tccgttacac tagaaaaccg aaagacaata aaaattttat
    15241 tcttgctgag tctggctttc ggtaagctag acaaaacgga caaaataaaa attggcaagg
    15301 gtttaaaggt ggagattttt tgagtgatct tctcaaaaaa tactacctgt cccttgctga
    15361 tttttaaacg agcacgagag caaaaccccc ctttgctgag gtggcagagg gcaggttttt
    15421 ttgtttcttt tttctcgtaa aaaaaagaaa ggtcttaaag gttttatggt tttggtcggc
    15481 actgccgaca gcctcgcaga gcacacactt tatgaatata aagtatagtg tgttatactt
    15541 tacttggaag tggttgccgg aaagagcgaa aatgcctcac atttgtgcca cctaaaaagg
    15601 agcgatttac atatgagtta tgcagtttgt agaatgcaaa aagtgaaatc agctggacta
    15661 aaaggcatgc aatttcataa tcaaagagag cgaaaaagta gaacgaatga tgatattgac
    15721 catgagcgaa cacgtgaaaa ttatgatttg aaaaatgata aaaatattga ttacaacgaa
    15781 cgtgtcaaag aaattattga atcacaaaaa acaggtacaa gaaaaacgag gaaagatgct
    15841 gttcttgtaa atgagttgct agtaacatct gaccgagatt tttttgagca actggatcca
    15901 ggagaacaaa aacgattttt tgaggaaagt tataaattat tttccgaacg atatggcaag
    15961 caaaatattg cttatgcaac agttcataat gatgagcaaa cccctcacat gcatttaggt
    16021 gttgtgccta tgcgtgatgg aaaactgcaa ggaaaaaatg tgtttaatcg tcaagaactg
    16081 ttatggctac aagataaatt ccccgagcat atgaaaaaac agggttttga gttgaagcgt
    16141 ggtgaacgtg gctctgaccg taaacatatt gagacagcta aatttaaaaa acaaactttg
    16201 gaaaaagaga ttgattttct agaaaaaaat ttagcagtta aaaaagatga atggactgct
    16261 tatagcgata aagttaaatc agatttagaa gtaccagcga aacgacacat gaaaagtgtt
    16321 gaagtgccaa cgggtgaaaa gtccatgttt ggtttgggaa aagaaataat gaaaacagaa
    16381 aagaaaccaa ccaaaaatgt tgttatatcg gagcgtgatt ataaaaactt agtgactgct
    16441 gcgagagata acgataggtt aaaacagcat gttagaaatc tcatgagtac tgatatggcg
    16501 agagaatata aaaaattaag taaagaacat gggcaagtta aagaaaaata tagtggtctt
    16561 gtagagcgat ttaatgaaaa tgtaaatgat tataatgagt tgcttgaaga aaacaagtct
    16621 ttaaagtcta aaataagcga tttaaagcgt gatgtgagtt taatctatga aagcactaag
    16681 gaattcctta aggaacgtac agacggctta aaagccttta aaaacgtttt taaggggttt
    16741 gtagacaagg taaaggataa aacagcacaa ttccaagaaa aacacgattt agaacctaaa
    16801 aagaacgaat ttgaactaac tcataaccga gaggtaaaaa aagaacgaag tcgagatcag
    16861 ggaatgagtt tataaaataa aaaaagcacc tgaaaaggtg tctttttttg atggttttga
    16921 acttgttctt tcttatcttg atacatatag aaataacgtc atttttattt tagttgctga
    16981 aaggtgcgtt gaagtgttgg tatgtatgtg ttttaaagta ttgaaaaccc ttaaaattgg
    17041 ttgcacagaa aaaccccatc tgttaaagtt ataagtgacc aaacaaataa ctaaatagat
    17101 gggggtttct tttaatatta tgtgtcctaa tagtagcatt tattcagatg aaaaatcaag
    17161 ggttttagtg gacaagacaa aaagtggaaa agtgagacca tggagagaaa agaaaatcgc
    17221 taatgttgat tactttgaac ttctgcatat tcttgaattt aaaaaggctg aaagagtaaa
    17281 agattgtgct gaaatattag agtataaaca aaatcgtgaa acaggcgaaa gaaagttgta
    17341 tcgagtgtgg ttttgtaaat ccaggctttg tccaatgtgc aactggagga gagcaatgaa
    17401 acatggcatt cagtcacaaa agggttctgt tgcaaagttt taaataaaga ataaaatccc
    17461 ttacggtatc tatgatttaa gctgggattc ccaataatac cttgatttca gtacagaccg
    17521 aaaacccgaa gagagtgcct tcttttcggg ttttcttata taatcctcga atggcttcca
    17581 tgcctttaat cgtggtagag gcagtgcgta aacttcgata gaatttattg cgtctcttta
    17641 ctggacgatg gtcttgttca atcaaattat tcaggtattt aatggtacga tgttctgtcc
    17701 cttgataaaa gccgtattct tttagtttct taaaggcact tgtaatagag ggggctttat
    17761 ctgtgactac aaccttcggt tcatcaaact gcttcactaa ccgcttaaga aaagcatagg
    17821 ctgcttgtgt gtcccgtttt ttacgtaacc aaatatccaa ggttaaacca tctgcatcga
    17881 tggctcgata caaataatgc cattttcctt taattttgat gtacgtttca tccattttcc
    17941 atgaataaaa ggatttttta tttttctttt tccaaatttg atagagtagt ttgccatatt
    18001 cttgcaccca acgataaatc gtcgtatgag aaacgttaat gccacgatca tataagattt
    18061 cttgaacttc acgatagcta aggttataac gaagatagta gcccacggct acaataatca
    18121 catcctgctg aaattgcttt cctttaaaat gattcatcgt cattcctcct gctatctttt
    18181 tctattattc taccttattt gatagtagat ttaaaacttt gcaacagaac cgttcgtcac
    18241 atcttcgatc aatttctctt acgattttct catccacatt tcttattctt acttccatat
    18301 taattcctcc aatttaaaaa ggatagcgaa aaggagccta tcctttttgc tatcctacta
    18361 ccctcctttt actatccttc actatccttt cactatccta tttttaaata ttttgttccc
    18421 acatttattt ttgagaaacg taataggcac agcctattgg aacacttttt ttaaaaagtg
    18481 ttcccacgtt tccttatgct c
//
