LOCUS       NZ_CP096968            11693 bp    DNA     circular CON 07-JUL-2023
DEFINITION  Phocaeicola vulgatus strain CL10T00C06 plasmid pBCPT_CL10, complete
            sequence.
ACCESSION   NZ_CP096968
VERSION     NZ_CP096968.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27738859
            Assembly: GCF_024022975.1
KEYWORDS    RefSeq.
SOURCE      Phocaeicola vulgatus
  ORGANISM  Phocaeicola vulgatus
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Phocaeicola.
REFERENCE   1  (bases 1 to 11693)
  AUTHORS   Evans,J.C., Laclare McEneany,V., Coyne,M.J., Caldwell,E.,
            Sheahan,M.L., Von,S., Coyne,E.M., Tweten,R.K. and Comstock,L.E.
  TITLE     A proteolytically-activated antimicrobial toxin encoded on a mobile
            plasmid of Bacteroidales induces a protective response
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11693)
  AUTHORS   Coyne,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) University of Chicago, Duchossois Family
            Institute, 900 East 57th Street, KCBD 4240, Chicago, IL 60637, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9
            Assembly Name                     :: Phvul_CL10T00C06_PBv1
            Genome Coverage                   :: 17
            Sequencing Technology :: PacBio Sequel II; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/07/2023 04:58:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,321
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 4,110
            CDSs (with protein)               :: 4,110
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 44 of 99
            Pseudo Genes (incomplete)         :: 60 of 99
            Pseudo Genes (internal stop)      :: 19 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096968.1.
            Sequencing by the Biopolymers Facility, Harvard Medical School,
            Department of Genetics, 77 Avenue Louis Pasteur, Boston, MA 02115,
            USA (Illumina) and by SNPsaurus, Institute of Molecular Biology,
            1318 Franklin Blvd, Room 273 Onyx Bridge, Eugene, OR 97403-1229 USA
            (PacBio). Gene calling method: Prodigal version 2.6.3. Annotated
            using a custom modification of Prokka version 1.14.6. Assembled
            using PacBio HiFi (css) reads. Snippy v. 4.60 was used to map both
            PacBio css and Illumina MiSeq reads to the assembly as final
            polishing steps. Genome coverage reports PacBio css read coverage
            as determined by the Flye assembler.
            Bacteria and source DNA available from Dr. Laurie Comstock,
            University of Chicago, Duchossois Family Institute (DFI), 900 East
            57th Street, Chicago, IL 60637 USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11693
                     /organism="Phocaeicola vulgatus"
                     /mol_type="genomic DNA"
                     /strain="CL10T00C06"
                     /isolation_source="human feces"
                     /db_xref="taxon:821"
                     /plasmid="pBCPT_CL10"
                     /geo_loc_name="USA:Boston"
                     /collection_date="2008-05"
     gene            1..978
                     /locus_tag="M0N98_RS21490"
                     /old_locus_tag="M0N98_04331"
     CDS             1..978
                     /locus_tag="M0N98_RS21490"
                     /old_locus_tag="M0N98_04331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669318.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_032943496.1"
                     /translation="MAKKDQKKRDIILSQDNALTTARYKFDLIEKRALYCIIRNIRANY
                     IEQEDGQRTLFENLIITIKEEELQKCGDNTKMKNIYDALKRLRNRDIEINNEKMWLSIG
                     FVNYVKHIKNKSYFEVEVSKEVLPYYVELAQNFTSYSMTVAIALKSTFTQRFYELCCQY
                     QNKGFFFYAVNELREMFMLENKYPRSFDFKKWVLETPAKELKNLYDAGQCDLYFDFDIR
                     EKKGKEALSYNFFIHSRQTEQQKLLEYESAKQAIIYITTTISRFSKSDKGYVKRVVNAV
                     QLHPEIAVQVAQKIQKKFEQYIDKPAKQIGAIIRVCLMEDFGIE"
                     /name="replication initiation protein"
     gene            992..1183
                     /locus_tag="M0N98_RS21495"
                     /old_locus_tag="M0N98_04332"
     CDS             992..1183
                     /locus_tag="M0N98_RS21495"
                     /old_locus_tag="M0N98_04332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008782581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_007851405.1"
                     /translation="MKTGDKVIVPAEINGYGRDLQAMVTEIEKFAGTIFVTVVFTEPCP
                     EAYGRKGGVYHDFQLITE"
                     /name="hypothetical protein"
     gene            1285..1806
                     /locus_tag="M0N98_RS21500"
                     /old_locus_tag="M0N98_04333"
     CDS             1285..1806
                     /locus_tag="M0N98_RS21500"
                     /old_locus_tag="M0N98_04333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_007851403.1"
                     /translation="MEKGQESKQKRKTPLTTIAISQEYAQKLDEYLNGTGITRKEFVEL
                     SVDYFQRTGFDLRGEAFDLSPLEKVAGRLEQSAKIMEQHNEGTEAVRQLLLAVREQTAK
                     QLPAPELIAHAAEEKAKAEAKNEEQEREITRLRSENSALLEYKKKAHRELCRVRDEQKI
                     FGKIHVKTEL"
                     /name="hypothetical protein"
     gene            1827..3464
                     /locus_tag="M0N98_RS21505"
                     /old_locus_tag="M0N98_04334"
     CDS             1827..3464
                     /locus_tag="M0N98_RS21505"
                     /old_locus_tag="M0N98_04334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="WP_252773037.1"
                     /translation="MKKQSIYFLVIIILLVQTSCQQNNEEEDFFNNQITLLENNPRLYL
                     SKIDSTQVTNLNNSKEATHFLLVSLANYYVNNYYPPKEVLQKSIHIFTKKKLIQQQLES
                     LLFLAKTYKKEKNLKMEVQAIEKAIDIASQIEDKEWLCCLYGYLGDMYIRKYNMLKFIK
                     YQTLANQCIEDIAFRDMDISTQVQTAKSFLYIGNHKKSYELLNLIESSIDKNNIYYNEI
                     KCLQGITLFKTKQWALCIEKLQEAIILSQTDDFLFVCHSILTYCYYSINDLANANKHRK
                     LAIEYDTDSETNFAEIEFYKLCAEFARENNYIDNQIDCLYKAIERYEILLRNLNGSSLD
                     EAIQAYTHFCDKKNYEKKLSTYKYAALSSLFIASIGLLIYINKKRKQAYQIVALHQQIE
                     TLEGLNNIKDEAKMFILRDFEIAKQIAMLRYTQKEQSAKFIKELNRFNITQNNSLLTTQ
                     WDNFYKHIDLSFDNFYTLLKDNFSDLNEKELQLCCMMVAGFKTEEIAAIWMQSIFSVHK
                     YKTNIRKKLKTPEGANIIAFLRSTPPFQ"
                     /name="LuxR family transcriptional regulator"
     gene            3612..3920
                     /locus_tag="M0N98_RS21510"
                     /old_locus_tag="M0N98_04335"
     CDS             3612..3920
                     /locus_tag="M0N98_RS21510"
                     /old_locus_tag="M0N98_04335"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_244668137.1"
                     /translation="MIKKGFLLALFAIGVFANIFAQDTLSFKKTRDKNIYIEFLGASNL
                     IGVSFDSRFTPISPWGYRIGISYFQGGDSFIKASNSNRGLYFSLLKLTSSPLEISTN"
                     /name="hypothetical protein"
     gene            4200..4862
                     /locus_tag="M0N98_RS21515"
                     /old_locus_tag="M0N98_04336"
     CDS             4200..4862
                     /locus_tag="M0N98_RS21515"
                     /old_locus_tag="M0N98_04336"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_244668136.1"
                     /translation="MKKISYIICLTAILCSCNNELPIENVQELQTPKSKEDSEILSFKN
                     SEEFLSTIENFDLDNPILTRASSSFVSAENIYEQENNENADNEYIGFLIPDEKYRNFFN
                     KDLEIIINDTLYKATKYGTLFTHISNKSELIKSLENISEFKPLSNNTKQYGNIKLIDTY
                     GLWDKETNNPITDDSFFENDEDDLLVENNGTETLTRSASDGGIPKSVVESFPTVDTN"
                     /name="hypothetical protein"
     gene            complement(4936..5691)
                     /gene="istB"
                     /locus_tag="M0N98_RS21520"
                     /old_locus_tag="M0N98_04337"
     CDS             complement(4936..5691)
                     /gene="istB"
                     /locus_tag="M0N98_RS21520"
                     /old_locus_tag="M0N98_04337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288784.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="WP_004288784.1"
                     /translation="MKSEKETIYDYAAELKLLAFKEELECTLSLAAEENWNHLQFLTEL
                     LGKESARRRECRRRSRIRSAGFPQMKYLHELVMEDMPKEAQVILPELETLDFIRQGRNL
                     VLYGNPGTGKTHIATALGIKACQQDFTVLFTSVPVLLTQIREAKSAKTLRTLQLRFEKY
                     DLVICDEFGYVSCDKEGGELLFNHLSLRAGKKATIITTNLAFNRWNEIIKDKVLVAAMV
                     DRLTHKAYLVNMTGLSYRLKETQKMRQDK"
                     /name="istB"
     gene            complement(5703..7265)
                     /gene="istA"
                     /locus_tag="M0N98_RS21525"
                     /old_locus_tag="M0N98_04338"
     CDS             complement(5703..7265)
                     /gene="istA"
                     /locus_tag="M0N98_RS21525"
                     /old_locus_tag="M0N98_04338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845533.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="WP_244668139.1"
                     /translation="MYRVCGYSKRRISRELHVSRHTVDNILSKYESAIRTDNPEEALSD
                     LLTIQPRYDSSRRRPRRLTQEIKDKIGFCLKKNAVKIATGLRKQRMLKKDIHQFLLSQG
                     YTISYATVCSYIKNIESYKEKKKSETFIRLFYEPGCIAEFDWGEVLLFIDGVKTKFYLA
                     VFTFGHSNGRYAYLFRHQNTLAFMESHRNFFRDIHGVPAMMVYDNMRVAVKSFVGGDKK
                     PTETLMKMSGFYCFEYRFCNVRAGWEKGHVERSVEYVRRKAFCLTDHFGDIHSAQEHLN
                     RVCMQVNNEQGSLSTAEKTSRLEADLSSLKPFPGNLGCFEVYEYIVDKWSTISMKNVHY
                     SVPDSLVGEKVHVKVYSEKIVILYGKEKVASHQRSYCGGDWCIKLEHYLRTLSRKPGAL
                     PHSVVWQRAPEELRRLYDIHFKEDNRTFVLLLDYARKNGFSGTDIVTACKELTGRGVRK
                     ISPDQVKAMLHGSVQGETEETMEPPVLPAQQENIEREAVDMLEGITALMTGYNEVHDII
                     PTI"
                     /name="istA"
     gene            7579..8262
                     /locus_tag="M0N98_RS21530"
                     /old_locus_tag="M0N98_04339"
     CDS             7579..8262
                     /locus_tag="M0N98_RS21530"
                     /old_locus_tag="M0N98_04339"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_252773036.1"
                     /translation="MKKLWHGMKWGHMKHWDDGIYYGVASLVVKQKIKNPSFDTVLDEN
                     RKEFLKKQWQEAIKHNNFTNASNSLTGGISNQWNPEYNHSKQENKFIIPYISVIDDFFG
                     TTNISKKIEDALIRQGANFLKSYVNSHKTQSTQLVCISQREKTAYSLFRNDLSWNGGGY
                     RVKETFLRYYRNIVFSIKIKGDKTTPGADLGDNDFVGKPEIVSCEAIVYTKDGDGWIGA
                     KIEKK"
                     /name="hypothetical protein"
     gene            complement(8680..9558)
                     /gene="btgB"
                     /locus_tag="M0N98_RS21535"
                     /old_locus_tag="M0N98_04341"
     CDS             complement(8680..9558)
                     /gene="btgB"
                     /locus_tag="M0N98_RS21535"
                     /old_locus_tag="M0N98_04341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein BtgB"
                     /protein_id="WP_007851393.1"
                     /translation="MNVKIQGGGNGTYANTGSCVAVTNYLQHEDLERMKKGEEVQPFFN
                     QFRDYVSAREVTFKIDNNKAKLSRTDAKFYVITVSPSEKELRCMGRTPQERAEALQRYI
                     RQDVMRNYAEGFGKGLRSDDVEYYAKIHFNRDGDSDSDMHAHIIVSRKDRSNTKKLSPK
                     TNHTGKKNCGNVKGGFDRTDFFRKCESSFDRCMGFDREPEESFDYLNAVKNGSPAEIAR
                     QVERAERIRKEKWDNLKAELQSRQEPEKSKIQQVEKTPDIEQPIPKKKQQEEDLELLKK
                     PKRSRGFGMGM"
                     /name="btgB"
     gene            complement(9555..10160)
                     /gene="btgA"
                     /locus_tag="M0N98_RS21540"
                     /old_locus_tag="M0N98_04342"
     CDS             complement(9555..10160)
                     /gene="btgA"
                     /locus_tag="M0N98_RS21540"
                     /old_locus_tag="M0N98_04342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein BtgA"
                     /protein_id="WP_007851392.1"
                     /translation="MDKETTTSVTIDRKTFARLDRLAKSNNVSKKDFLSCALEYFEKYG
                     INPVEHESPAKEMQKLIKRCDQVIAFIRKQEQDFLRPACEAMGSTSMRVTMSMDSILTE
                     KKFSQYQKDNDLFMRDLASLAGIREQALDRAEKVVGQSRDMLLKNQQAIYARLEAVTQR
                     QEKIFSYIASYIDAKGKAGLFDDIKALYKDEKNKREKI"
                     /name="btgA"
     gene            10552..10899
                     /locus_tag="M0N98_RS21545"
                     /old_locus_tag="M0N98_04343"
     CDS             10552..10899
                     /locus_tag="M0N98_RS21545"
                     /old_locus_tag="M0N98_04343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_007851390.1"
                     /translation="MEAKSRFKVKLSNEAQEFLDGLPNKVREKIISNIRKSTFVLDPDL
                     FKKLDDTDIWEFRTFYNKTKYRLLAFWDKGDKINTLVIATHGFIKKTQKTPLKEIAKAE
                     EIRKDYFNSKR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            10905..11219
                     /locus_tag="M0N98_RS21550"
                     /old_locus_tag="M0N98_04344"
     CDS             10905..11219
                     /locus_tag="M0N98_RS21550"
                     /old_locus_tag="M0N98_04344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851388.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_007851388.1"
                     /translation="MEAKKFYTLEEIEDKYIGEKGTPKRDAYEEELNSFLIGEAIKQAR
                     QSKNLTQEELGNLIGVQRAQISRIENGKNLTFSTIARVFKAMGISAKLEIGSMGKVALW
                     "
                     /name="helix-turn-helix transcriptional regulator"
     Typing          230..978
                     /name="rep_cluster_1097"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8684..9558)
                     /name="MOBB"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.918"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaaga aagaccaaaa gaaacgagat attatcttat ctcaagataa tgcactcaca
       61 actgcccgtt ataagttcga ccttatcgaa aagcgtgcgt tatattgcat cattcgcaat
      121 atacgggcaa actatatcga acaagaggac ggacaaagaa ctttgtttga aaatcttata
      181 atcacaatca aagaggaaga acttcaaaaa tgtggtgata atactaaaat gaagaatatc
      241 tatgatgcgc taaagcggct tagaaatcgt gatatagaga ttaataacga aaagatgtgg
      301 ctgagtatcg gttttgtcaa ttacgttaag catatcaaaa ataaatcata ttttgaagtg
      361 gaagtatcca aagaggtact accctattac gtggaactcg cccaaaactt cacgagttac
      421 tccatgacag tagcaatcgc actcaaaagc acatttaccc aacgtttcta tgaactatgc
      481 tgccaatatc aaaataaagg tttctttttc tatgctgtca atgagctacg ggaaatgttc
      541 atgttggaaa acaaatatcc acgttctttc gattttaaaa aatgggtact tgaaacgcca
      601 gctaaagaat taaaaaacct atatgatgcc ggacaatgtg atttatactt cgatttcgac
      661 ataagggaaa agaaaggtaa agaagcactc tcttataact ttttcataca cagccgtcaa
      721 accgaacaac aaaagctatt ggaatacgaa agtgccaaac aagcgatcat ctacataaca
      781 accactattt cgagattctc taaaagtgac aaaggatatg ttaaacgtgt agtgaacgct
      841 gtacagttac acccggaaat agccgtacag gtggcgcaaa aaatacaaaa gaagtttgag
      901 caatatatag acaagccagc caaacaaata ggagctatca tccgggtttg cttaatggaa
      961 gattttggta tagaataaaa aacatacaag tatgaaaaca ggtgataaag ttatcgtgcc
     1021 agccgaaatt aacggctatg gcagggattt acaggcaatg gttacagaga tagagaagtt
     1081 tgctgggaca atattcgtaa cagtggtatt caccgaacca tgtccggaag cctacggaag
     1141 aaaaggaggc gtttaccatg acttccagct aataacagag tgatttattt tgctataatc
     1201 gcaataaatt gctataacac atgtaataaa ttgcaataat aaaacttata actacctttg
     1261 taacaaaata tagcatttac agcaatggaa aaaggacagg aaagcaagca aaagaggaaa
     1321 acaccgctta ccaccatagc gataagccaa gagtacgccc aaaaattaga cgagtattta
     1381 aacggtacgg gtataactcg caaagaattt gtcgagttgt ccgttgatta tttccaaaga
     1441 actggattcg accttcgagg tgaagcattt gacctctccc cattagaaaa ggttgccggg
     1501 cggctggaac aatctgcaaa gatcatggaa cagcacaacg agggaacaga agcggtacgc
     1561 cagctattgc tagcagtccg ggaacagacc gctaaacagc ttccggcacc agaactgatt
     1621 gcccatgctg ccgaagaaaa agcaaaggca gaagccaaaa acgaagaaca agaaagggag
     1681 attacccggt tacgctctga gaactctgct ttgctagaat ataagaaaaa ggcacatcga
     1741 gaactgtgtc gggtacgtga cgaacaaaaa attttcggaa aaatacatgt aaaaactgag
     1801 ttgtaatcaa acctattgat aatactatga aaaaacaatc tatatatttt ttagttatca
     1861 tcattttgtt agtacagaca tcttgccaac aaaacaatga ggaagaagac ttttttaata
     1921 atcaaataac actcttagaa aataatcctc gattatatct ttctaaaatt gattcaaccc
     1981 aagtcactaa tcttaataac tcaaaagaag caactcattt cttattagtt tcattagcca
     2041 actactatgt aaataattac tacccaccca aagaagtatt acaaaaaagt attcatattt
     2101 tcacaaaaaa gaaactaatt caacaacaac ttgaatccct tttgtttctt gccaaaacat
     2161 acaaaaaaga aaaaaactta aaaatggagg tgcaagctat agaaaaagct atagatatag
     2221 caagccaaat agaggataaa gaatggctat gttgtctata tggctatcta ggagatatgt
     2281 atatccgcaa atacaacatg ttgaaattta tcaaatatca aacattagct aaccagtgta
     2341 ttgaagatat tgcatttagg gatatggata tatcaactca agtacaaacg gcaaaaagtt
     2401 ttttatatat aggaaatcat aaaaagtcct acgagctatt gaatttaata gaaagctcca
     2461 ttgataaaaa caacatttat tataatgaga taaaatgcct acaaggtatt acgctattta
     2521 agactaaaca atgggcacta tgtatcgaaa aattacagga agctataata ttaagccaaa
     2581 cagatgattt tttgtttgtc tgccattcta tactaactta ttgctattac tccataaatg
     2641 atttagcaaa tgctaataaa catagaaaat tagctataga atatgataca gattcggaaa
     2701 ccaattttgc tgaaatagaa ttttataaac tgtgtgcaga atttgctaga gaaaataatt
     2761 atatagacaa tcaaattgat tgcctgtata aggcaataga acgatatgaa attcttctaa
     2821 ggaacttgaa tggaagttct ttagatgaag ctattcaagc atatactcac ttttgcgata
     2881 aaaaaaatta tgaaaagaaa ttatctacat acaaatatgc tgctttaagt tctcttttta
     2941 tagcaagcat agggctatta atctacatca ataaaaaaag aaaacaagct taccaaattg
     3001 tggctcttca ccaacaaata gaaacattgg aaggcttaaa taatataaaa gatgaagcaa
     3061 aaatgttcat cttacgtgat tttgaaatag ccaaacaaat tgcaatgttg agatatacgc
     3121 aaaaagaaca aagtgctaaa tttataaaag aattaaacag attcaatatt acacaaaata
     3181 attctttact aactacgcaa tgggacaatt tttataaaca cattgattta tcttttgata
     3241 atttttatac actgctgaaa gataactttt cagatctaaa tgaaaaagaa ctacaacttt
     3301 gctgtatgat ggttgcaggg ttcaagacag aagaaattgc agctatatgg atgcaaagta
     3361 ttttttctgt acacaaatat aaaaccaata tccgaaagaa acttaaaact cctgaaggtg
     3421 ctaatataat agctttttta aggtctactc ctccattcca atgacagctt atacttttac
     3481 aatctttttt ttgtgatgca ccttaactat caaatagtta tatattaagt ttacaaattc
     3541 acaaaaaagc ttacaaatgg cgatttgaag tattaagtct aactttgcaa cgttcaaaat
     3601 caataaatta tatgataaaa aaaggttttt tacttgcatt gtttgctata ggtgtttttg
     3661 caaatatatt cgcacaggac accttatctt tcaaaaagac gagagataaa aatatctata
     3721 tagagttttt aggagcttct aaccttattg gagtttcttt cgattcaaga tttactccta
     3781 tatccccatg gggatatagg attggtattt cttattttca aggaggggat tcttttatta
     3841 aagcatctaa ctctaataga gggctctatt tttccctatt gaagttaact tcctcacctc
     3901 tggaaataag cacaaattag aacttggatt aggatctaat cttggaatat ataacgaaca
     3961 catttctttt atagaagaat caaaggaaca atatgaaaca ataagttcca cttccaatac
     4021 aacatttgga tattatttct tttctaatat aggttaccgt tacatatcaa ctaaaggttt
     4081 tctatttaga ataggattaa gcccttcttt tagttttaat gataaacacg gtataacaaa
     4141 agaaccattt atttaccctt atattagttt tggatattca ttttaataaa ttcacatata
     4201 tgaaaaaaat cagttatatc atctgtttaa cagctatttt gtgttcatgt aacaatgaac
     4261 ttcctattga aaatgtacaa gagctacaga ctcctaaatc aaaagaagat tcagaaattc
     4321 ttagttttaa aaattcagaa gagttcttat caacaataga aaacttcgac ttggataatc
     4381 ctattttaac aagagcaagt tcatcttttg tttctgcgga aaatatctat gaacaggaaa
     4441 acaatgaaaa tgcagataat gaatatattg gttttcttat tcctgatgaa aaatatagaa
     4501 attttttcaa taaagatcta gaaataatta tcaatgatac tctttacaaa gcaactaaat
     4561 atggtacact atttactcat atttcaaata aatcagaatt aatcaaatct ttagaaaata
     4621 tttctgaatt caaaccgtta tccaataata cgaaacaata tggaaacatt aaacttatag
     4681 acacttacgg actatgggat aaagagacta acaatcctat tacagatgat agtttttttg
     4741 aaaatgacga ggatgattta ttagtagaaa acaatgggac tgaaactctc acacgttccg
     4801 caagcgatgg aggtatacct aaatctgtcg tagaatcatt ccccactgta gatactaatt
     4861 gaaaagtgcg ctgtattcta attgaaaaga gctccatcca taacttgtta caaaattact
     4921 ataagtttaa aatattcatt tatcttgtct cattttttgt gtttccttaa gcctataaga
     4981 cagtccggtc atattaacca gataagcttt atgtgtaagc ctgtcaacca ttgccgccac
     5041 aagcaccttg tcctttatga tttcattcca tctgttaaaa gccaaattag tagtaatgat
     5101 tgtagccttt tttccggctc ttaacgacag gtggttaaaa agcagttctc ctccttcctt
     5161 gtcacaactg acatatccga actcatcaca gatgaccaga tcgtattttt caaaccttaa
     5221 ttgtagcgtc ctcagtgtct ttgctgattt agcctccctt atctgggtaa gcaggaccgg
     5281 cactgaagta aacaatacgg taaagtcctg ttggcaggcc tttattccta aagccgtagc
     5341 aatatgcgtc tttcccgttc ccggatttcc atacaggacc aggtttcttc cctgtctgat
     5401 gaagtccagt gtctccaatt caggtaatat tacctgtgcc tctttgggca tgtcttccat
     5461 aacaagctca tgcagatact tcatttgtgg aaatcccgca gatcttatcc ttgatcttct
     5521 tctacactcc cttctcctgg cgctttcctt tccaagcaat tccgtcaaga actgcagatg
     5581 gttccagttt tcttcagctg ccaatgaaag ggtgcattcc agttcctctt taaaggccag
     5641 aagcttcagt tctgcagcat agtcataaat ggtttctttt tctgatttca tatattataa
     5701 acttaaatgg ttggtattat atcatgcact tcattgtatc ctgtcatgag cgccgtgatg
     5761 ccttcaagca tatccacagc ttctctttct atattctcct gttgtgccgg gagaacaggc
     5821 ggttccatgg tttcttctgt ctctccctgt acgctaccat gcagcatggc ctttacctgg
     5881 tccggagata tctttctgac accacgtccg gtcagctcct tgcatgccgt gacaatgtct
     5941 gttccggaaa atccattttt tcgggcatag tccagcaaca gaacaaacgt cctgttgtcc
     6001 tccttgaaat ggatgtcata cagccttctc agttcttccg gtgctctttg ccaaaccaca
     6061 gagtggggca atgcccccgg tttacgggaa agtgtacgca aatagtgctc cagcttgatg
     6121 caccagtctc caccgcaata actgcgttga tgagaggcca ctttctcctt cccgtacagg
     6181 atgacgattt tttcactata aaccttgaca tgtacttttt ctcccacaag agaatcaggt
     6241 acggaataat ggacattttt catgctgata gttgaccatt tatccacaat gtactcatag
     6301 acctcaaaac agcccagatt accgggaaaa ggcttcagcg atgacaggtc agcttccaga
     6361 cgtgatgttt tctccgctgt tgaaagactg ccttgctcgt tgttgacctg catacatacc
     6421 cggtttaaat gctcctgggc agaatgtata tcaccaaaat ggtctgtcag gcagaaagct
     6481 ttccttctga catattccac gctgcgctct acatgtcctt tctcccatcc ggcccgtaca
     6541 ttacagaaac ggtactcaaa acaatagaaa ccggacatct tcatcaaagt ttctgtaggt
     6601 ttcttatcac caccgacaaa gctcttgacg gctacacgca tattgtcata gaccatcatg
     6661 gcggggacac catgtatatc cctgaaaaag ttacggtggg attccatgaa ggcaagcgta
     6721 ttctgatgcc tgaaaagata ggcgtatctg ccattgctat gcccgaaagt gaatacggcc
     6781 agataaaact tggttttgac gccgtcaata aaaagaagaa cttcacccca gtcaaactcg
     6841 gcaatgcatc caggctcata gaacaaccgg ataaaggttt cgctcttttt cttctctttg
     6901 tatgactcta tattttttat ataactgcat actgtggcgt aactgatggt gtatccttga
     6961 gataacagaa actggtggat atcctttttc aacatgcgct gtttgcgaag tccggtagct
     7021 atcttaacgg cattcttctt caggcaaaat cctatcttat ccttaatctc ttgtgtgagc
     7081 cggcgaggac ggcgtctgga actgtcatac ctgggctgga tggtaagcaa atcactcaaa
     7141 gcctcttctg gattgtccgt gcggatggct gattcgtact ttgaaagaat attgtcaacg
     7201 gtatgacggc tgacatgaag ttcacgagag atacgccgtt tgctataacc gcatactcta
     7261 tacatgtgta ttattgattg tctttctacc atagtcttca ttttaccttt gcatttggat
     7321 tatacaaagg tcattatact tatcctatgg tggcgcaatt ttcaactgga atattggcgc
     7381 acttttcaat tagtatctac attccccaca ataggaacag ctgatgtaaa ccttatagat
     7441 aaggtcatac actttaatcc gggctatcta ggacatacga aattaagatt taaatctaat
     7501 tcaaaaagaa aactatatat ttctctatac agaaatgact atggatttgc agtaagtatt
     7561 ggacttgatt gtaaggtcat gaaaaagtta tggcatggta tgaaatgggg acacatgaaa
     7621 cattgggatg atggtatata ctatggagtt gcatcactcg ttgtaaaaca aaaaatcaag
     7681 aatcccagtt ttgatactgt actagatgaa aatagaaaag agtttctaaa gaaacaatgg
     7741 caagaagcta ttaagcacaa taatttcact aacgcttcaa acagtctaac tggaggaatt
     7801 tcaaatcaat ggaatcctga atataaccat agcaaacaag aaaataaatt cattattcca
     7861 tatatttcag ttatagatga tttctttgga acaacaaata tatcaaagaa aatcgaagat
     7921 gctcttatac gtcaaggagc taatttccta aaaagctatg ttaatagtca taaaacacaa
     7981 agtacacaat tagtttgcat atcacaacga gagaaaactg catattcttt atttagaaat
     8041 gatctctctt ggaacggggg aggatataga gtaaaagaaa cattccttag atattataga
     8101 aacattgtat tttccattaa aatcaaagga gataaaacaa ctccaggagc agatctagga
     8161 gacaatgatt ttgttggtaa gccagaaatt gtatcctgtg aggctattgt atataccaaa
     8221 gatggtgatg gctggattgg agcaaaaata gaaaaaaaat aatcctaaac aaaataggga
     8281 aggagttaat atatacgctc cttcccttca cttattgtat gaaaaaaaag tatcttattt
     8341 ttttagtcct aagtttctgt ttgttctcgt catgctcaac aatgcttgaa ataataacag
     8401 gaaattataa atgcgcatac cctaattgta atgaacgagc aacagaaaat tcagtatatt
     8461 gcccatacca taagccctac tatcttaaat agcatcatca ccaatgtttt ttcttttttc
     8521 ctaaacacct gccagcgaag cacagagtat ttgtcaaggg cgaagggtaa aaaataccgc
     8581 acgagcgaag cgagcacagg agatttttta cccggagagt gaaacgtccc tttacaaata
     8641 ctctgtgcgg agttaccttt gtgccggaaa atagaaaaat cacatgccca ttccgaaacc
     8701 tctgctcctt tttggcttct tcaggagttc cagatcttcc tcctgctgct tctttttggg
     8761 aataggctgc tcgatgtccg gcgttttctc cacctgttga atttttgact tctccggttc
     8821 ctgccgggat tgaagctccg ctttgagatt gtcccatttc tcctttctga tgcgttccgc
     8881 acgttccacc tgccgggcta tttccgccgg gcttccgttc ttgacagcgt taagatagtc
     8941 gaagctttcc tccggctccc ggtcaaaccc catgcaccga tcaaaagagc tttcacactt
     9001 ccggaaaaaa tccgtccggt caaaacctcc cttcacgttg ccgcagttct tctttccggt
     9061 gtggttcgtt ttagggctta actttttggt gttgctccgg tctttccgac tgacgatgat
     9121 atgtgcgtgc atatcgctgt cactgtcacc gtcccggttg aaatggatct ttgcgtaata
     9181 ttccacatca tcgcttctca agccttttcc gaaaccctct gcatagtttc tcatcacgtc
     9241 ctgccggatg taccgctgca aggcttccgc acgctcctgc ggagtcctgc ccatgcagcg
     9301 cagctccttc tctgaagggc tgacggtgat aacatagaac tttgcgtcag tccggcttaa
     9361 cttcgccttg ttgttgtcta tcttgaatgt gacctccctt gcgctcacgt agtcccggaa
     9421 ctggttgaag aacggctgca cctcctcccc tttcttcatc ctctccagat cctcatgctg
     9481 gagatagttc gtcaccgcaa cacaactgcc tgtattggcg tatgtgccgt taccgcctcc
     9541 ctgtatcttc acgttcatat cttttccctt ttgttcttct cgtccttgta caaggctttt
     9601 atatcatcga aaagcccggc ttttcctttc gcgtcgatgt agcttgcgat ataggagaag
     9661 atcttctcct gcctttgcgt cacagcctcc agccttgcat aaatggcttg ttggttcttc
     9721 agaagcatat ccctcgactg accgactacc ttttccgccc ggtccaaagc ctgctccctt
     9781 atcccggcaa ggcttgcaag atcacgcatg aataggtcat tgtccttttg gtattggcta
     9841 aacttttttt ccgtaagtat cgagtccatg ctcatggtca ctctcatgct tgtactgccc
     9901 atggcttcac aagccggacg caagaaatcc tgctcctgct tcctgatgaa cgctatcacc
     9961 tgatcacagc gtttgataag tttctgcatt tcctttgccg gactttcatg ttcaaccggg
    10021 ttgatgccgt acttctcgaa gtattccagc gcacaggaaa ggaagtcttt ctttgataca
    10081 ttattggact ttgccagcct gtcaagccgg gcaaacgttt ttcgatcaat ggtgacagaa
    10141 gtggttgttt ctttatccat acaattgcga ttttaattgc taattatatt attaaatctc
    10201 tgatttacaa aggtataatt ttaaccacaa tttcaaacta attgtggtta aaaaacttaa
    10261 acggctgata ttcagccgta atcccgtagg gcaagaggaa aaacagggca aagcccggtt
    10321 tcctcttgct gttcttttcg actgccgagc aatcgacgaa caccctcccc tgcggtacgg
    10381 cgaaagctcg tacaaggggg acgaggagaa gtcggagcga ggcactaaga aagaacctat
    10441 tccacaaaca aaaaacacat caaaacggca caacaggctg cataaaataa atatattttg
    10501 tttcgttata tggaaacaaa ttgttatatt tgcaacacta aaaaacttta tatggaagca
    10561 aaatcaagat tcaaggtcaa attatccaac gaagcccaag agtttttaga cggattgccc
    10621 aacaaggtac gtgagaagat cattagcaat attcgtaaat ccacctttgt acttgatcct
    10681 gatttattca agaaattgga tgatacggat atctgggagt tcagaacgtt ctataacaaa
    10741 accaagtaca ggcttcttgc tttttgggat aagggcgata agataaacac tctggtaatc
    10801 gcaacgcacg ggtttatcaa gaaaacccaa aagacacccc tcaaagaaat agccaaagcc
    10861 gaagagatta gaaaagatta ttttaattca aaaagataaa gcgtatggaa gcaaagaaat
    10921 tttatacact cgaagaaatc gaagataaat acatcggaga aaaaggtact cccaaacgtg
    10981 atgcctacga agaagaactt aactcttttt tgataggtga agccattaaa caggctcgcc
    11041 aatcaaagaa cttaacccaa gaggaattag gcaatttgat aggagtgcag cgggctcaaa
    11101 tctcccgaat tgaaaacggt aagaatttaa ccttttccac cattgcaaga gtttttaagg
    11161 ctatggggat aagtgctaaa cttgaaatcg gaagcatggg taaagttgca ttatggtaac
    11221 tgactatatc cgggatgcgt tttcgctccc ggaaaacttc tcatattaca cacaaaaaat
    11281 catatttata caaattttaa cacgcggaaa aatcctctca atccgttgca cttggcttta
    11341 cctatttcca ctctcttcct ttcttcggaa atttatcgta tcaaaaacaa ggtgaaaagt
    11401 ggagcgaagc aacctctata aaccaaaaaa gaaacgctcc attgctttac ggtaattttt
    11461 agtttctttt tagttacttt ttcggcatgt ttttttgctt tgcaatatat ttatattcaa
    11521 tgagttacaa agtgtcattt ttgagaaaaa cgagaaattt ccgagagaaa acgagaaatt
    11581 tccgagagaa aacgagaaat ttccgttttt ttttgagaaa aacgagaaaa aaccgcgaga
    11641 gttttttttt tttctcgttt tatatatttt attttgtgga aaatattcgg cgt
//
