LOCUS       NZ_CP096944             2783 bp    DNA     circular CON 14-JUN-2024
DEFINITION  Pseudomonas aeruginosa strain NY5524 plasmid pNY5524-NR2, complete
            sequence.
ACCESSION   NZ_CP096944
VERSION     NZ_CP096944.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28004303
            Assembly: GCF_030121935.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Zhou,D.
  TITLE     complete sequence of strain NY5524
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2022) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            No.20,Dongdajie,Fengtai, Beijing, Beijing 100071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030121935.1-RS_2024_06_14
            Annotation Date                   :: 06/14/2024 07:01:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,960
            CDSs (total)                      :: 6,879
            Genes (coding)                    :: 6,770
            CDSs (with protein)               :: 6,770
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 53 of 109
            Pseudo Genes (incomplete)         :: 58 of 109
            Pseudo Genes (internal stop)      :: 12 of 109
            Pseudo Genes (multiple problems)  :: 12 of 109
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096944.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="NY5524"
                     /isolation_source="phlegm"
                     /host="Homo sapiens"
                     /db_xref="taxon:287"
                     /plasmid="pNY5524-NR2"
                     /geo_loc_name="China:HeNan"
                     /lat_lon="36 N 110 E"
                     /collection_date="2019"
                     /collected_by="a public hospital"
     gene            191..550
                     /locus_tag="M2I87_RS34830"
                     /old_locus_tag="M2I87_34690"
     CDS             191..550
                     /locus_tag="M2I87_RS34830"
                     /old_locus_tag="M2I87_34690"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_112302719.1"
                     /translation="MAIKQKQSNLFDDYMERRFEEMAWADARNNDEDRQPVTVRLPISL
                     VRKIDFLAKSLDHSRQVFLDDLIGLAVAQALNAYVGAHAEGEARDRAHAEVAELYQSAS
                     REEAEALFGVLKEAK"
                     /name="hypothetical protein"
     gene            550..867
                     /locus_tag="M2I87_RS34835"
                     /old_locus_tag="M2I87_34695"
     CDS             550..867
                     /locus_tag="M2I87_RS34835"
                     /old_locus_tag="M2I87_34695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044061178.1"
                     /translation="MEEAMFTLCGQVAMVFVQPGGVSKKTGEEFEARDKVQILGKLPIP
                     GGGTKLELIDLVTDDIQAFKSLHGKQIRVPVGVYAVRGSVGYFIPKGSKPALVKAQVPN
                     A"
                     /name="hypothetical protein"
     gene            967..2220
                     /locus_tag="M2I87_RS34840"
                     /old_locus_tag="M2I87_34700"
     CDS             967..2220
                     /locus_tag="M2I87_RS34840"
                     /old_locus_tag="M2I87_34700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431321.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_270006072.1"
                     /translation="MQVLSSGLPEKGISMRARKNKAPGSTESILADGQVLQGADETVGF
                     PARIARYAVAKKRAVTMRDYLEGRSAQNAVKARAGLSACGEWLHFREYYTVDKIRLHAA
                     SFCKQHLICPLCAIRRGAKSLAAYLARYEVIRAQHPHLSPYLVTFTVKNGPDLAERFQH
                     LHKGLRRLLDRRRNFSAGVRAAPWTELCLAEGAVYTFEVTNKGNGWHPHVHMIALCPRE
                     PSQEALRSEWERITADSFMVDVRAFDASQEPVEGFMEVFKYAVKFSDLSPEDNWHAAQV
                     FKGRRLLGSFGLFRGVEIPESMADEPLDGLPYVDRFYRFLQGEYQFTGESPRSVDEETE
                     GGGGYQPTPEGERLFPLPAGPELPLRHENSRRGAKTTPKSMATLWWEAEERRKRNAQRR
                     KDKLNSLDDAGAKAKEDG"
                     /name="protein rep"
     gene            2316..2594
                     /locus_tag="M2I87_RS34845"
                     /old_locus_tag="M2I87_34705"
     CDS             2316..2594
                     /locus_tag="M2I87_RS34845"
                     /old_locus_tag="M2I87_34705"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_270006073.1"
                     /translation="MAKPYPIVPLSWLDVFNDLNNQLVAYEMAVQALFRQFDAHGSPYE
                     LETVLSGLHDLVQPVIEGYQSMADQCQVARDLGMVGLASIEDRDASR"
                     /name="hypothetical protein"
ORIGIN
        1 cttgacggat accccaccca ataccctcca ggcacctccg ggcagcacag ctgcccgggg
       61 gatgccgccc ggcgagggcg ctcctgctgt tgggttgctg cggtaaagct gcgtatttaa
      121 atccatagtg aatcttgtat gattcatctg tcgcgataat tcggtccgac agaaatcatc
      181 ggagattcat atggctatca agcaaaagca gagcaacctg ttcgacgact acatggagcg
      241 tcgattcgaa gagatggcat gggctgatgc acggaacaac gacgaagacc gccagccggt
      301 gacggtgcgt cttcccatca gcctggtccg caagatcgac ttcctggcca agagcctgga
      361 ccactctcgc caggtgttcc tggacgacct cattggcctg gcggttgccc aggcgctcaa
      421 cgcctatgtc ggggcgcatg ccgagggtga ggcgcgtgat cgtgcccatg ccgaagtggc
      481 cgagctgtat cagtcggcca gtcgcgagga agccgaagcg ctcttcggcg tgctgaagga
      541 ggccaagtga tggaagaggc catgttcacc ctctgcggcc aggtggcgat ggtgttcgtc
      601 cagcctggcg gcgtcagcaa gaagaccggc gaggagttcg aggcgcggga caaggtgcag
      661 atcctcggca agctgccaat ccctggcggc ggtaccaagt tggagctgat cgatctggtc
      721 accgatgaca tccaggcgtt caagtcgctg catgggaaac agatccgcgt gccggtcggc
      781 gtttacgccg ttcgcggcag cgtcggttac ttcattccga agggctccaa gcctgcgctg
      841 gtcaaggctc aagtgcccaa tgcgtaagcg ttgcagggtg ttcaccccgg ccggatggct
      901 gctcgttccg agctccgccg tccggggtga acaccctgct ttgcttgata catagaaaca
      961 ttgtgcatgc aggtgctaag ctcaggcctg ccggagaaag gaatttcgat gcgggccaga
     1021 aaaaacaaag cccccggtag cactgagtcg atcttggcgg acggacaggt gctacagggg
     1081 gccgatgaaa ccgttggctt ccccgccaga atagcacgct acgccgtcgc caagaaacgc
     1141 gcggtgacga tgcgtgacta ccttgagggg aggtcagccc agaacgctgt gaaggcacgg
     1201 gcgggcctct ctgcctgcgg cgagtggctc cacttccggg agtactacac cgtggacaag
     1261 attcggctgc acgcggccag cttctgcaag cagcacctga tctgcccgct ctgcgcgatc
     1321 cgccgcgggg cgaagtccct ggccgcctac ctggcgcgct acgaagtgat ccgtgcgcag
     1381 cacccccacc tctcccccta cctggtcacg ttcacggtga agaacgggcc ggacctggcc
     1441 gagcgtttcc agcatctgca caaggggctt cgccggctac tcgaccgtcg ccggaatttt
     1501 tccgccggcg ttcgagcagc tccgtggact gagctgtgct tggccgaagg cgccgtgtac
     1561 accttcgagg tgacgaacaa gggcaacggc tggcatccgc acgtgcatat gatcgcgctc
     1621 tgccctcggg aaccctctca ggaggcccta cggagcgaat gggagcgaat tacggctgac
     1681 tccttcatgg tggacgtccg agcgtttgac gcctcacagg agcccgtaga gggctttatg
     1741 gaggtcttca agtacgcggt gaagttctcc gacctgtcgc cggaggacaa ctggcatgcc
     1801 gcccaggtct tcaagggtcg tcggctgctg ggcagcttcg gcctgttccg tggcgtcgag
     1861 attccggagt cgatggctga cgaaccgctc gacggcctgc cctacgtgga tcggttctac
     1921 cggttcctcc agggcgagta tcagttcacc ggtgaatcgc ccaggtcggt cgatgaggag
     1981 accgaggggg gaggggggta tcagcccacc ccagaagggg agcggctgtt cccgcttcca
     2041 gctggacccg aattaccgtt acgtcacgaa aattccaggc ggggagcgaa gaccacgccg
     2101 aagtcgatgg ccaccctgtg gtgggaggcc gaagagcgcc gcaagcgcaa tgcgcagcgc
     2161 cgcaaggaca agctgaactc gttggatgac gccggagcga aggcgaagga agacgggtag
     2221 tcgaatttcc gtgacgtcac ggaaaagtcg ccggtaggcg ggaggtcaga ggcccgccca
     2281 ccggctaacc acagtgatct gaaggagaga ccaccatggc taagccatat cctatcgtcc
     2341 cgctgtcttg gttggacgtt ttcaacgatc tcaacaacca attggtcgcc tacgagatgg
     2401 ccgtccaggc gctgtttcgg cagttcgatg cgcacggatc gccctacgag ctggagacgg
     2461 ttctgtcggg tctgcatgac ctggtgcaac cggtgatcga ggggtatcag tcgatggccg
     2521 atcagtgcca ggtcgctcgc gacctgggga tggtcggatt ggcctcgatc gaggatcgtg
     2581 acgcgtcacg gtaattccgg cgctatgacc gtgcagtgca tttgccacgc agatgcactg
     2641 cacctgctat ggccagccgc tgtaggcacg tgaccgaccg tttacggtcg atgtgcccag
     2701 ggacaccctg gaacccgtca ggggccgccg caccggctcg tcgcgacttg tcgcgtcgaa
     2761 cggagcgccg ggcgaagcga acc
//
