LOCUS       NZ_CP096943             2953 bp    DNA     circular CON 14-JUN-2024
DEFINITION  Pseudomonas aeruginosa strain NY5524 plasmid pNY5524-NR1, complete
            sequence.
ACCESSION   NZ_CP096943
VERSION     NZ_CP096943.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28004303
            Assembly: GCF_030121935.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Zhou,D.
  TITLE     complete sequence of strain NY5524
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2022) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            No.20,Dongdajie,Fengtai, Beijing, Beijing 100071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030121935.1-RS_2024_06_14
            Annotation Date                   :: 06/14/2024 07:01:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,960
            CDSs (total)                      :: 6,879
            Genes (coding)                    :: 6,770
            CDSs (with protein)               :: 6,770
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 53 of 109
            Pseudo Genes (incomplete)         :: 58 of 109
            Pseudo Genes (internal stop)      :: 12 of 109
            Pseudo Genes (multiple problems)  :: 12 of 109
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096943.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="NY5524"
                     /isolation_source="phlegm"
                     /host="Homo sapiens"
                     /db_xref="taxon:287"
                     /plasmid="pNY5524-NR1"
                     /geo_loc_name="China:HeNan"
                     /lat_lon="36 N 110 E"
                     /collection_date="2019"
                     /collected_by="a public hospital"
     gene            join(2938..2953,1..335)
                     /locus_tag="M2I87_RS34815"
                     /old_locus_tag="M2I87_34675"
     CDS             join(2938..2953,1..335)
                     /locus_tag="M2I87_RS34815"
                     /old_locus_tag="M2I87_34675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="WP_077265610.1"
                     /translation="MSNETKEKIFMKKTFALAAMMAAIAPNLASAADEMIQTKYACEGG
                     KTLDVVYVGDYAVMLQMDELVPLKRAKSASGVRYISASKNYTYELWGKGNDMNLSTHDG
                     GKEQTVLSSCKP"
                     /name="MliC family protein"
     gene            complement(362..1336)
                     /gene="mobV"
                     /locus_tag="M2I87_RS34820"
                     /old_locus_tag="M2I87_34680"
     CDS             complement(362..1336)
                     /gene="mobV"
                     /locus_tag="M2I87_RS34820"
                     /old_locus_tag="M2I87_34680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_033995413.1"
                     /translation="MAAYAIMRCKKLATMGSVAAALQHCYRERETPNADAERTPDNEHR
                     AATSTDAAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWAKATPQQQAAFFDQAQGWLA
                     AKYGADRIVTASIHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRDKMRADQTSFAEAVR
                     DLGLERGIEGSRATHQRVQSYYGAIERQPGHATITPQAIEPRVLRKGIFSKDVETPEAV
                     AARVTAAVREGYGPTVAAAAGARQEREKARQAQETARSLRDRLKPVLDALGPLNRDMQA
                     KAAQIIKAVGEKLLAEQREATRQRQAQRLRSRGRDRDDGGLSR"
                     /name="mobV"
     gene            2390..2941
                     /locus_tag="M2I87_RS34825"
                     /old_locus_tag="M2I87_34685"
     CDS             2390..2941
                     /locus_tag="M2I87_RS34825"
                     /old_locus_tag="M2I87_34685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA plasmid replication protein"
                     /protein_id="WP_033995415.1"
                     /translation="MQQITVATDAQRRIRQRELAMLDEKEAQEREEARKNKGFTQVYPL
                     GWKRIIELAKGNAGAFGLYSFFAENIDPTCGAVVCDQQFLADKMNVNRRTISRWLSYLE
                     ENRALVRIPVAGKVCAYALDPHEVWKGYDNAKDYAAFVTKTLVNKDGDIRRRIMSMFSG
                     EKQNDDRDPNTIDMFESTPQ"
                     /name="RepA plasmid replication protein"
ORIGIN
        1 aggagaaaat ctttatgaaa aaaacgttcg ctcttgctgc catgatggcg gcaatcgctc
       61 cgaatcttgc ttcagcagcg gatgagatga tccagaccaa gtacgcttgc gaaggcggaa
      121 agacgttgga tgtcgtgtac gtcggagact atgccgtgat gctgcaaatg gatgagcttg
      181 tcccactgaa acgcgccaag agcgcatcag gagtgcgcta catctccgct agcaaaaact
      241 acacctacga actgtggggt aagggcaacg acatgaacct ttcgacccac gatggcggga
      301 aggagcaaac cgtcctcagt agctgcaagc cataaacccc ttccgtcggc cacctgtagg
      361 gtcaacgaga cagcccgccg tcgtcgcgat cccggccgcg actgcgaagc cgttgggcct
      421 gccgctggcg tgtcgcctcg cgctgttcgg cgagcagctt ctcgcccacg gccttgatga
      481 tctgggccgc cttggcctgc atatcgcgat taagcggccc cagagcgtcc agaacgggct
      541 tcaggcggtc acgcaggctc cgggctgtct cctgtgcctg ccgggccttc tcgcgctcct
      601 gacgcgctcc tgcggccgct gcgacggtcg ggccataccc ttccctgacg gcagcggtca
      661 cgcgggcggc cacggcctcc ggcgtctcca cgtccttcga gaaaatcccc ttgcgcagca
      721 cccgcggctc gatggcctgc ggcgtgatgg tggcgtgccc gggctggcgc tcgatggcgc
      781 cgtagtagct ctggacgcgc tgatgggtgg cccggctgcc ctcgatgccg cgttccagcc
      841 cgagatccct caccgcctcg gcaaagctgg tctggtcggc ccgcatcttg tcgcggccac
      901 cgatgaactc cttggccgac agccgcccgt cctgcgtcag cggcacgacg aaggccgaca
      961 ggtgcggggt ggcctcgtcg cggtggatgc tggccgtcac gatgcgatcc gcgccgtatt
     1021 tggccgccag ccagccctgc gcctggtcga agaacgcggc ctgctgctgg ggcgtggcct
     1081 tggcccacca ttccgggctg gccgtcatca cgtattcgac cgccagcacc gcgtccttgc
     1141 gccgcttctc cggcagcaac tcgcgcagcc gccccatcgc cgcatcggtt gaggtggccg
     1201 cccggtgctc gttgtccggc gtgcgttcgg cgtcagcgtt gggcgtttcg cgctcgcggt
     1261 agcagtgctg caacgccgcc gccacgctgc ccatcgtcgc cagcttcttg catcgcatga
     1321 tcgcgtaggc cgccatgcct gcccctcccc ttggtgtccg aatccggcca gtcggggcag
     1381 cgcaaggcgg tgcctccggc gggacactat cagatgatag tgtactcact agactttgcg
     1441 aagcaaagtc gtgcccgcct gcggcggttg cagcgccctt cgggcttgct cctgcggcgg
     1501 ccttcggcct tgcccaaagg ggctttgccc ctctggactc cccaccctcg ccgccgggag
     1561 gctcgggggg caggggtggc gggcttcgcc cttcgtccct gcccccactc gcacaggctg
     1621 gggcgctgcg gtcagcgtca agggttcggc ttcgcccgcg tcctcgcccg gtccctgcgc
     1681 ttcgctccgg gctgcggcct gcgggcgcgc ccttgaccct gccctccgct gaaggcgtca
     1741 gccagtggcc cgctggcgtg tcggaaatcg cctcatggcc gggtaggaaa tttccgacag
     1801 cgccggagtg gaaggcgggt caagggcgca gggggtggct ggcgcggccc gcagcccgca
     1861 gggcgaggac acgggccacc cccggagagc gcagcggccc ttgacgcgcc tggaacgacc
     1921 ccacgctccc cggtaggggt gcaggagtga agggccgcag gcccgtagcc cctgcacccc
     1981 tgccacgtgg cgaacgcggg gggtggcggg gggcagcgcc caccgcccca aggctgcgca
     2041 gcggccgccg gaggcccccc aaacaacgtc acccggccgg tggccgggtg ggccttttcc
     2101 agaggccccg caggggccgt cagcggggtg ccaaggccgc gtagcggccg ccggcggggc
     2161 ggcagcccct tagaggagaa agggggaaat gctaagttat tgatttataa ggatttgtag
     2221 aaatgcattg agacacatag ggtgtcctaa cgttgagaca tgcatggatg tctcaacagg
     2281 tagcaaggtt gagacgcaaa ctgtctcgac gtgagacata atttgtcgta tcattgcgac
     2341 tcgaaatggc gcaatgagcg gacaaatttc ccgactttgg aggaacccca tgcagcagat
     2401 cacggtggcg accgacgccc agcgtcgtat ccgccagcga gagctggcca tgctggacga
     2461 gaaagaggct caggagcgcg aagaggcgcg gaagaacaag gggttcacgc aggtgtatcc
     2521 cttgggttgg aagcgcatca ttgagcttgc taaaggcaat gctggggcct tcggcctgta
     2581 ttcattcttt gctgaaaata tcgacccaac ctgcggcgcg gtggtctgcg atcagcagtt
     2641 tttggcagac aagatgaacg tcaacaggcg gacaatcagc cgttggttgt cttacctgga
     2701 agaaaatcgc gcgcttgtcc gcatccctgt tgctggcaag gtttgcgcct atgcgctgga
     2761 tcctcacgag gtctggaagg gctacgacaa cgccaaggat tacgcagctt tcgtgaccaa
     2821 aaccttggtc aacaaggatg gcgatatccg ccgccggatc atgtcgatgt tcagcggcga
     2881 gaagcagaac gacgaccgcg acccgaacac cattgacatg tttgaaagca ctccccaatg
     2941 agcaacgaaa cga
//
