LOCUS       NZ_CP096900             5503 bp    DNA     circular CON 05-JUN-2024
DEFINITION  Acinetobacter baumannii strain Mu1956 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP096900
VERSION     NZ_CP096900.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27988045
            Assembly: GCF_030168285.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Choby,J.E. and Weiss,D.S.
  TITLE     Heteroresistance via beta-lactamase gene amplification threatens the
            beta-lactam pipeline
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Choby,J.E. and Weiss,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) Division of Infectious Disease, Emory
            University School of Medicine, 954 Gatewood Road Room 2052, Atlanta,
            GA 30329, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030168285.1-RS_2024_06_05
            Annotation Date                   :: 06/05/2024 05:01:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,207
            CDSs (total)                      :: 4,111
            Genes (coding)                    :: 4,017
            CDSs (with protein)               :: 4,017
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 59 of 94
            Pseudo Genes (internal stop)      :: 16 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096900.1.
            Bacteria and source DNA available from David S Weiss, Emory
            University, dweiss8@emory.edu.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="Mu1956"
                     /isolation_source="respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA:Georgia,Atlanta"
                     /lat_lon="33.7911 N 84.3233 W"
                     /collection_date="2012/2015"
                     /collected_by="Georgia Emerging Infections Program"
     gene            join(5490..5503,1..1093)
                     /locus_tag="M1783_RS21030"
                     /old_locus_tag="M1783_21020"
     CDS             join(5490..5503,1..1093)
                     /locus_tag="M1783_RS21030"
                     /old_locus_tag="M1783_21020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995413.1"
                     /GO_function="GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA]; GO:0051903 -
                     S-(hydroxymethyl)glutathione dehydrogenase (NAD(P)+)
                     activity [Evidence IEA]"
                     /GO_process="GO:0006113 - fermentation [Evidence IEA];
                     GO:0009404 - toxin metabolic process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="WP_284627638.1"
                     /translation="MKSLIVAFGPGQPLQIVEVDVAPPKKGEVLIKISHTGVCHTDAFT
                     LSGDDPEGVFPAILGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECVFCKSGKSNL
                     CISVRETQGRGVMPDGTTRFSYNGQPIYHYMGCSTFSEYTVVAEVSLAKINPEANHEHV
                     CLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGGIGLAVVQAARQAKAGRIIVVDMNPDK
                     FALAKEFGATDFLNPKDYDKPIQQVIVEMTGWGVDHSFECIGNVNVMRAALESAHRGWG
                     QSVIIGVAGAGQEISTRPFQLVTGRKWLGSAFGGVKGRTQLPGMVEDAMKGKIQLEPFV
                     THTMGLDQINEAFDLMHEGKSIRTVIHY"
                     /name="S-(hydroxymethyl)glutathione dehydrogenase/class III
                     alcohol dehydrogenase"
     gene            1146..1544
                     /locus_tag="M1783_RS21035"
                     /old_locus_tag="M1783_21025"
     CDS             1146..1544
                     /locus_tag="M1783_RS21035"
                     /old_locus_tag="M1783_21025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="WP_000235176.1"
                     /translation="MATPVISGNDIFSHVFLGAVDLKKSVVFYDAALGALGIKNLGPFG
                     NDWVLYGRDKPAFMITRPSNGAEPTSNGVTVGFAAATPAEVNAFHAAGLAAGGTDEGQP
                     GPRGHLPGAYAAYLRDPAGNKVCAYTFV"
                     /name="VOC family protein"
     gene            2018..2851
                     /gene="fghA"
                     /locus_tag="M1783_RS21040"
                     /old_locus_tag="M1783_21030"
     CDS             2018..2851
                     /gene="fghA"
                     /locus_tag="M1783_RS21040"
                     /old_locus_tag="M1783_21030"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439570.1"
                     /GO_function="GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="WP_000439570.1"
                     /translation="MERIEHHASFEGWQDVFQHESTVLGCMMRFGIYLPPQAEHEKLPV
                     LYWLSGLTCTEQNFITKAAVQRYAAEHGIIVVAPDTSPRGESVADDAAYDLGQGAGFYV
                     DATELPWSDHYQMYSYVVDELPALIEANFPASSRRSISGHSMGGHGALVIALRNPGRYA
                     SVSAFSPIVAPTQVPWGQKALAAYLGEKNLEAWKQYDTVELIRVAQERLPLLVDQGLSD
                     EFLEHQLRPQLLRTACEQVDHPLTLNLRPGHDHSYYFISTYLSEHMAHHAAALNR"
                     /name="fghA"
     gene            complement(3607..3879)
                     /gene="nadS"
                     /locus_tag="M1783_RS21045"
                     /old_locus_tag="M1783_21035"
     CDS             complement(3607..3879)
                     /gene="nadS"
                     /locus_tag="M1783_RS21045"
                     /old_locus_tag="M1783_21035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_000369780.1"
                     /translation="MDNNLFDDLVASIKEAGAIKRKEIKASRVTELELPDIKEVREKTG
                     LTQAEFAARLHISARTLQNWEQGRRYPTGPAATLIRILDAHPTLI"
                     /name="nadS"
     gene            complement(3872..4192)
                     /locus_tag="M1783_RS21050"
                     /old_locus_tag="M1783_21040"
     CDS             complement(3872..4192)
                     /locus_tag="M1783_RS21050"
                     /old_locus_tag="M1783_21040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000897309.1"
                     /translation="MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGI
                     RKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAYPKSVKDNLTDKETSILRQLVKEQF
                     HG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4379..4576)
                     /locus_tag="M1783_RS21055"
                     /old_locus_tag="M1783_21045"
     CDS             complement(4379..4576)
                     /locus_tag="M1783_RS21055"
                     /old_locus_tag="M1783_21045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000476222.1"
                     /translation="MFKQTLSVKDQFGVKHAIQATVDHEFANTQSHLNIKHITVDGEDI
                     RPSFEMLFQSTLSGKIFKII"
                     /name="hypothetical protein"
     gene            complement(4643..4870)
                     /locus_tag="M1783_RS21060"
                     /old_locus_tag="M1783_21050"
     CDS             complement(4643..4870)
                     /locus_tag="M1783_RS21060"
                     /old_locus_tag="M1783_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000921865.1"
                     /translation="MLKVNDLIAKSKNGTEIIVSLIPLHKMQNTRQGLKQIEVGKRVLL
                     ESGIEVDLNLDGRTFYTSLNQLFKLNHKVA"
                     /name="hypothetical protein"
     gene            complement(4969..5184)
                     /locus_tag="M1783_RS21065"
                     /old_locus_tag="M1783_21055"
     CDS             complement(4969..5184)
                     /locus_tag="M1783_RS21065"
                     /old_locus_tag="M1783_21055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004693381.1"
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]; GO:0003723 - RNA binding [Evidence IEA]; GO:0001072 -
                     transcription antitermination factor activity, RNA binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="WP_000071892.1"
                     /translation="MSNSNVVKGTVKWFNETKGFGFIQQESGPDVFAHFSEIASSGFKT
                     LFEGQQVEFSVAQGQKGPNAVNIVAI"
                     /name="cold-shock protein"
ORIGIN
        1 tgttgcgttt ggtcctggtc aacctcttca aattgttgag gttgatgttg caccaccgaa
       61 gaaaggtgaa gtattaatca agatctccca tactggtgtg tgtcatacag acgcatttac
      121 tttatctggc gatgatcctg aaggcgtttt tccggctatt ctcgggcatg aaggtgctgg
      181 tgttgtagtt gaggttggtg aaggcgtgac tagcgtcaaa ccaggtgatc atgttattcc
      241 actttacact gctgaatgtg gcgagtgcgt attctgtaaa tctggaaaat cgaacttgtg
      301 tatttccgtt cgcgaaactc aaggtagagg cgtaatgcca gatggtacca ctcgcttttc
      361 ctacaatggt caaccgattt accactatat gggctgctca acgttcagtg aatacactgt
      421 agtggcagaa gtatcattgg ctaaaatcaa tcctgaagcc aatcacgaac atgtctgctt
      481 actgggttgt ggggtaacaa ccggtatcgg tgcagtacat aacacggcta aagtacaaga
      541 aggtgatagt gttgcggtct ttggtttagg tggtattggt ttggcagtcg tacaagccgc
      601 acgtcaagct aaagcagggc gtattattgt ggttgacatg aatccagata aatttgcgct
      661 ggcaaaagag tttggtgcaa cggacttctt gaaccctaaa gactacgata aaccaatcca
      721 gcaagtcatt gtcgaaatga ctggctgggg tgtagatcat tcatttgaat gtattggcaa
      781 cgtgaatgta atgcgtgcag cccttgaaag tgcacaccgt ggctggggac aatccgtaat
      841 tattggtgta gctggcgcag gtcaggaaat ctctacccgt ccattccaac tggttactgg
      901 tcgtaaatgg ctaggttctg cctttggtgg cgtaaaaggt cgtacgcaac ttccaggaat
      961 ggttgaagat gccatgaaag gtaaaattca attagaacca tttgtgacac atacgatggg
     1021 cttggatcag atcaatgaag cctttgatct aatgcatgaa ggtaagtcaa ttcgcactgt
     1081 cattcactat tgatccacgg atcaatacct aatttttcaa aaccaaccat caaggaaaca
     1141 ctttaatggc tactccagta atttccggta acgatatttt ctcccatgtc ttcctcggcg
     1201 cagtcgacct taagaagtcg gttgtattct acgatgccgc tctaggtgcc ctcggcatta
     1261 aaaacctagg gccattcggt aacgattggg ttctgtacgg tcgcgacaaa cctgctttca
     1321 tgatcacccg cccaagcaat ggcgcagaac ccaccagtaa tggtgtgacg gtaggcttcg
     1381 cagctgccac ccctgcagag gtaaatgcat tccacgcagc cggcctcgcc gctggtggca
     1441 ccgatgaagg tcagcctggc ccacgtggcc acctgccggg tgcttacgcg gcctacctgc
     1501 gtgatccggc tggtaataag gtctgcgcct acacctttgt ctaaaaccta ggagaagtga
     1561 ggtcattgtg taaggactgg ttccagccaa aaacaatctc atcttccact gcaaccgtgt
     1621 ggcatgcccg ctcatgccac acggtttacg gtactctgag cctctatctg ctgattagag
     1681 ctcagaaagg ctgttacctc gccttcatag ccaaagacgg tcaatgagca cgatcggact
     1741 gaaagttcgg tgcgtgatgc aagaaacaca gaatccaaaa tgagcatcgc ggtagtggct
     1801 ccctgaatta cacactttat tgtttgtaca ttgaagtcga catccgacct tcgtggtgtg
     1861 gtggcgtctg ggtttgtagc agcgatcttc atgccagcga caacgctgaa aacctgctga
     1921 gtggtgcggt gaagcatttt taactaatcc tggatggaac gaaaattcgt cttgattggc
     1981 gcctgagcac cacctcgttg aaataaggag aaaatccgtg gaacgcattg aacatcacgc
     2041 cagcttcgag ggttggcagg acgtttttca gcatgagtca acagtgcttg gctgcatgat
     2101 gaggtttgga atctatcttc cgcctcaggc tgagcatgaa aagctcccag ttctgtactg
     2161 gctctcaggt ctgacctgta ccgaacagaa cttcatcacc aaggctgcgg tgcagcggta
     2221 tgccgcggag cacggaatca ttgtcgtagc cccagatacc agcccgcgcg gagaaagcgt
     2281 tgcagacgat gctgcctatg atctaggtca aggtgcgggt ttctatgtag acgccacaga
     2341 gctaccttgg agcgatcact atcagatgta ttcctatgtg gtcgacgaat taccagcgct
     2401 gatcgaagcg aacttccctg cgtcgtcaag acgcagcatc agtgggcact ccatgggcgg
     2461 tcatggcgct ctggttattg cgcttcgcaa ccccggtcgc tatgcaagtg tctcggcgtt
     2521 ctcgccgatc gtggcgccta cgcaagttcc atgggggcag aaggctctgg cggcttactt
     2581 gggcgaaaag aatctggaag cctggaagca atatgacacg gtcgagctga ttcgcgtggc
     2641 gcaagaacgc ttaccgctgc ttgtcgatca agggctgagc gatgagtttc tcgagcacca
     2701 gcttcgaccg caactgttgc gtaccgcgtg tgaacaggta gaccacccgc taacgctcaa
     2761 tctgcggcct ggtcatgacc atagctacta ctttatttcg acctacttga gcgaacacat
     2821 ggcccaccat gcggctgcat tgaatcgctg aacattggga gaccctgact tggaaatgtc
     2881 tgtataggac atttctattt ggttttaagg ggctttgttg cataaataca taaccaactg
     2941 attttgttca tttaattttt aggttgaaat tcatacaaac ctatttaaat cagaagctta
     3001 caaaatcttt atgcaacaaa gcccatatca gataaaaaag tagaggagct aatactaagc
     3061 cgtgatttct tggaaaagct gaaagagcta gttaatcgaa cagaaatacc atgtagttaa
     3121 ctatccattt aacataatgg gcgttatccg aaatcgtctt ttataagtta aataaaatca
     3181 tgtacttagc tattttacat aacccaaaac taccgcaaaa atccgctttg gaaacaaagc
     3241 ggattttttt tgagcaagtt tgatacttct tgccaataaa attggttaaa taatgatcaa
     3301 aaatagcaat tccatagagt ttttaagcct tttttaccca aaagaatcca caatttctgc
     3361 ggataaccat tgggataaat tttgattatt ttttgtacgt tttggcttac agcgtttcac
     3421 gaaattgtct gtatctatcc cccgatgaag ctcttatgat tgatctatat ggacaggatg
     3481 ttcaatgact taaaacaaca ataataatca gtcaggcacc atgaccatca ggtagtcagc
     3541 cctacctatg cgctccatac cgaaaaatcc cgacatgacg tcgggatttt ttattatcta
     3601 aacttcttaa attaaagttg gatgagcatc taaaatacga attaaagtcg ctgcaggacc
     3661 agtcggatag cgacggcctt gttcccagtt ttgcagggtt ctcgcgctga tatgcaaacg
     3721 tgcagcaaat tcagcctggg ttaagcctgt tttctcacgt acttctttaa tatccggtaa
     3781 ttcaagctct gtgactcgac ttgcctttat ctccttacgc ttgatggcac cagcttcttt
     3841 gattgaagca accaggtcat caaataagtt gttatccatg aaattgctcc tttactaatt
     3901 gacgtagaat cgaggtttct ttgtctgtca gattatcttt cacagacttt ggataagcca
     3961 ccaggaaaaa gatctgatca tcttctgtaa cccaataata gatcacacgg ataccgccac
     4021 ttttgccttt attgccctga gcacaacgta ctttacgaat tccaccacca ttcttgatta
     4081 agtcgccctt atcaggctgt accaagagat cttgctggag ctgacgatac tcttcatcac
     4141 tcacaagctc tttgatttgc ttagtaaaaa tactggtttc aatgaataac ataggtttaa
     4201 gtacgtcaat ggcgtataag aattttagca gttttagaat aaaaaaacga gagctgtgaa
     4261 ctctcgtttc gcataagcaa aggcttacaa atataaatat taggtataaa aaacccgcat
     4321 gatagcgagt aggtcctgat atttcattca gactatagat tttataaaaa ctatatttct
     4381 agatgatttt aaatattttt ccacttaagg tactttgaaa aagcatttca aaactaggtc
     4441 gaatatcttc accatcaacg gtgatatgtt ttatattcaa atgagattgt gtgttggcaa
     4501 actcatgatc cactgtggct tgaatagcgt gtttaacgcc aaactggtct tttacgctga
     4561 gagtctgttt aaacatatat atttccttaa aaaaattgca gtggaaatcc cacataaatt
     4621 attgatgttt aaaaatttat cgctaagcaa ctttatgatt taatttaaat agctgattta
     4681 gagaagtgta aaatgtacgg ccatccaaat ttaaatcaac ttcgattcca gattccagta
     4741 gaactctttt acctacttcg atttgtttta aaccttgacg tgtattttgc attttatgta
     4801 gaggaattaa tgaaacaata atttcagtac catttttaga ttttgcaatt aaatcattta
     4861 cttttaacaa cttatatcct attttttaga gattttttca attttgaaaa cattttaaag
     4921 cttaaaaaaa gcacttatga gtgctttttt attactgctt attattactt agatagcaac
     4981 aatatttaca gcattcggtc ctttttgacc ttgagctaca ctgaattcaa cttgctggcc
     5041 ttcaaataag gtcttgaaac ctgaactcgc gatttcgctg aaatgagcaa aaacgtctgg
     5101 acctgattct tgttgaatga aaccaaaacc tttagtttcg ttaaaccact taacagtacc
     5161 tttaacaaca tttgagtttg acataatata tcctactaat ttttaataat tttttagtca
     5221 ttttattgac tgattaataa ctttgaaaat aataaagaat gagacttaaa atctaaaaaa
     5281 cgaaggatta tgactaaaac tgcgatactt aaggaagatt taccgaaaca agactttttt
     5341 tctagttaag ttaaatatac agtaaaaata aaaataatca agttttttga tataaatatt
     5401 aatttttctt ataaaatcca tatttttttc tttaaaaatt aattaaattc aataagttat
     5461 ttattatatt atttaatatt ttctttaaca taaaatctct tat
//
