LOCUS       NZ_CP096898            10966 bp    DNA     circular CON 05-JUN-2024
DEFINITION  Acinetobacter baumannii strain Mu1956 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP096898
VERSION     NZ_CP096898.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27988045
            Assembly: GCF_030168285.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 10966)
  AUTHORS   Choby,J.E. and Weiss,D.S.
  TITLE     Heteroresistance via beta-lactamase gene amplification threatens the
            beta-lactam pipeline
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10966)
  AUTHORS   Choby,J.E. and Weiss,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) Division of Infectious Disease, Emory
            University School of Medicine, 954 Gatewood Road Room 2052, Atlanta,
            GA 30329, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030168285.1-RS_2024_06_05
            Annotation Date                   :: 06/05/2024 05:01:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,207
            CDSs (total)                      :: 4,111
            Genes (coding)                    :: 4,017
            CDSs (with protein)               :: 4,017
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 59 of 94
            Pseudo Genes (internal stop)      :: 16 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096898.1.
            Bacteria and source DNA available from David S Weiss, Emory
            University, dweiss8@emory.edu.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10966
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="Mu1956"
                     /isolation_source="respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA:Georgia,Atlanta"
                     /lat_lon="33.7911 N 84.3233 W"
                     /collection_date="2012/2015"
                     /collected_by="Georgia Emerging Infections Program"
     gene            1..951
                     /gene="repM"
                     /locus_tag="M1783_RS20930"
                     /old_locus_tag="M1783_20920"
     CDS             1..951
                     /gene="repM"
                     /locus_tag="M1783_RS20930"
                     /old_locus_tag="M1783_20920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001208778.1"
                     /translation="MRELVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHAEGYINQFGVHRNTAYQALKDACNDLFARQFSYQKINERGNIENYRSRWVSEIGY
                     VDNEAVVKLIFAPAIVPLITRLEEHFTKYELQQVSNLSSAYAVRLYELLIAWRSTGSTP
                     VIELSDFRQRIGVLDTEYKRMERFKTSVLELAIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDNPPIERDPNTLDLFTKMTDAQRHMFANKLSELPEMGRYSQGTESYPQFAV
                     RIAEMLQDPDRIKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            944..1519
                     /locus_tag="M1783_RS20935"
                     /old_locus_tag="M1783_20925"
     CDS             944..1519
                     /locus_tag="M1783_RS20935"
                     /old_locus_tag="M1783_20925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096619.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSRQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            1539..1682
                     /locus_tag="M1783_RS20940"
                     /old_locus_tag="M1783_20930"
     CDS             1539..1682
                     /locus_tag="M1783_RS20940"
                     /old_locus_tag="M1783_20930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(1871..2389)
                     /locus_tag="M1783_RS20945"
                     /old_locus_tag="M1783_20935"
     CDS             complement(1871..2389)
                     /locus_tag="M1783_RS20945"
                     /old_locus_tag="M1783_20935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            2762..3049
                     /locus_tag="M1783_RS20950"
                     /old_locus_tag="M1783_20940"
     CDS             2762..3049
                     /locus_tag="M1783_RS20950"
                     /old_locus_tag="M1783_20940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            3036..3350
                     /locus_tag="M1783_RS20955"
                     /old_locus_tag="M1783_20945"
     CDS             3036..3350
                     /locus_tag="M1783_RS20955"
                     /old_locus_tag="M1783_20945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            3478..5889
                     /gene="znuD2"
                     /locus_tag="M1783_RS20960"
                     /old_locus_tag="M1783_20950"
     CDS             3478..5889
                     /gene="znuD2"
                     /locus_tag="M1783_RS20960"
                     /old_locus_tag="M1783_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            6194..6655
                     /locus_tag="M1783_RS20965"
                     /old_locus_tag="M1783_20955"
     CDS             6194..6655
                     /locus_tag="M1783_RS20965"
                     /old_locus_tag="M1783_20955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(7091..8058)
                     /locus_tag="M1783_RS20970"
                     /old_locus_tag="M1783_20960"
                     /pseudo=""
     CDS             complement(7091..8058)
                     /locus_tag="M1783_RS20970"
                     /old_locus_tag="M1783_20960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611607.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /name="IS30 family transposase"
     gene            complement(8160..8492)
                     /locus_tag="M1783_RS20975"
                     /old_locus_tag="M1783_20965"
     CDS             complement(8160..8492)
                     /locus_tag="M1783_RS20975"
                     /old_locus_tag="M1783_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000390243.1"
                     /translation="MEDSTLNELIRNTELFFEHHWNVDLLGKPPEWSPVHFNFGKIPNY
                     DKQGVYAFIKGDLVTYIGVGTSRGAGRYRGNGLSARVMKYCRWNEDKTEYIAIDPRLKD
                     AGSMAE"
                     /name="hypothetical protein"
     gene            complement(8510..8776)
                     /locus_tag="M1783_RS20980"
                     /old_locus_tag="M1783_20970"
     CDS             complement(8510..8776)
                     /locus_tag="M1783_RS20980"
                     /old_locus_tag="M1783_20970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050441450.1"
                     /translation="MSVTETLDSKIKAQEEKLKQLKAQRQAALARERTKEKEQARKDDT
                     RRKILIGSCMLKITEDDEQARAKLIAQMDKYLTDERDRKLFNL"
                     /name="hypothetical protein"
     gene            8986..10422
                     /gene="mobQ"
                     /locus_tag="M1783_RS20985"
                     /old_locus_tag="M1783_20975"
     CDS             8986..10422
                     /gene="mobQ"
                     /locus_tag="M1783_RS20985"
                     /old_locus_tag="M1783_20975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029315.1"
                     /translation="MAIYHFSVKTVARSAGRSATAAIAYRAGEKIYCEREGREHDYSRK
                     TGVEYKEIYLPEGAPEHLKNREKLWNEVEQRETRKNSTVAREFEIAFPSELNQEQRLAM
                     LEELCASIVERHQVAVDACIHAPHTGSGSDERNYHAHILMSTRKLTPEGFTEKTRELDQ
                     KHSGEIDHWREHFADICNVHLNLAGSTARVDHRSYKDQENGLEATLHEGPKVTELRRRG
                     IETEISRSNDEIKQRNQAQLQYDKNMDVLIAENEIKLSTLKTEQQIQIKNSAKTPPIDE
                     KSLFEEKQRGTLGKVLKREISAKDANLDLDFMQRNLKQAEINLTKHHKHQNEFNQQLAQ
                     EIVKSGLKQSHDKLQSLVDQHNELTQNKPLLFGKKAWEAQRDEIYQEHKKLKGQHEHQK
                     KHGVKDLLENEKFKEHAWKKYQKQHPEKAKQYQTLYQSYKIVKKCVDEIKAEQQMKIRQ
                     EQQLKAQQHAPKMKSRGMSR"
                     /name="mobQ"
     Typing          2..951
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8987..10419
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.213"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagagaat tagtggtaaa agacaatgcc ttaatcaatg caagctataa cttagattta
       61 gtagaacaac gtttaatttt attggccatt gttgaggcaa gagaaagcgg gaaaggtatt
      121 aatgcgaatg atccccttga agtacatgca gaaggctata tcaatcaatt tggcgtacat
      181 cgcaatacgg cttatcaagc attaaaagat gcctgtaatg atttatttgc aagacaattt
      241 agctatcaaa aaataaatga acgagggaat attgagaact atagatcccg ttgggttagt
      301 gaaattggat atgtagataa tgaagcagtg gttaaactta tctttgcccc agccatagtt
      361 cccttaatta cacgcttaga agagcatttc actaaatacg aattgcagca agttagtaat
      421 ctcagcagtg cttatgctgt tcgcttatat gaattattaa ttgcttggag aagtactggc
      481 tctactcctg ttatagagct aagtgatttc cgtcaaagaa ttggcgtact cgatacagag
      541 tacaagcgta tggaacgctt taaaactagt gtacttgagc ttgctattaa acaaattaac
      601 gaacatacag atatcactgt gaagtatgag caacacaaaa gaggtcgatc aatttcagga
      661 ttctctttta cttttaaaca gaaaaagaag gacaacccac cgatagaaag agatccgaat
      721 acgttagatc tctttacaaa gatgactgat gcacaacgcc atatgtttgc aaataaactt
      781 tctgaacttc ctgaaatggg tcgttattcc cagggaactg aaagctatcc gcaatttgct
      841 gttcgtattg ctgagatgct gcaagaccct gaccgaataa aagaactata cccataccta
      901 aaaaaagtgg gatatatgcc atcaaataaa aaggacaccg taaatggcta agttatcact
      961 aagtgaagta tctaaaaaat tccatgtgga tagatcaacc atttacagag ctgtacgtaa
     1021 tggacgttta tcacgctcca gtgatggaca attcgatcta gcagaggtca tccgatgctt
     1081 tggagaacct gagcaaacat ctcaaaaaat tgaatcatct aggcaagaag gtgatgaatc
     1141 tacaaaaaaa cttattgccc atttagaaaa tgaagtcaaa aaataccaag aacgtgaaga
     1201 acggttaatg caacaaattg accgtatgca aacactcatt gagctgaaaa gtgttgcacc
     1261 tgccacagca gcaccacacc aagatgctac ggcatgcgac accaagatgc cacagcatgc
     1321 gacaacacaa caagacactg ataacaaaaa gaataatgaa ttaaatattg cagaaaatgt
     1381 agcagtgcca cagcaagaaa ctacggcata ccacacccaa acgctacagc atgccacgtt
     1441 gcaaagtgtg gcagtgccac aacacaaaaa acgtggttta tttggccgtg tgctgaatgc
     1501 cgtctttgat aatgactgag caataagggt gaaagatcat gccgaaactg aaagacattg
     1561 ccctgggaat tattgtagct ccgctgctga tcccgatcat gctgattgca tcgtaccagg
     1621 ataaaaaggc gctgaagaaa gagctggatg aatgccaaaa agaaaaagac caggcatcct
     1681 gattattcaa ttgagctaat caaaagtgtg gaacattgat gaaataatag cgtggaacgt
     1741 gaaaagtcat aaaaaaagcc cgaacaggga tgctcgggct ataaactaga aactacagcg
     1801 ttgaattata aggataatta tacagcattt cgtataaggt gtattatgtt aattttagga
     1861 aatattaaaa ctaattttca gacttatccc accaactttg agctaaatca gtatttttag
     1921 gaatacctat accatcacga tacataacgg aaagacttcg cattgctttc ttattaccta
     1981 actttgcagc cttttcacga agttcaaaag ctttattaat attaacttct attccgctat
     2041 ctgcacgagt atatagatca gctaactcaa ccattgattc agaatggcca ttttctgagg
     2101 ctttctgaaa ccacttaaaa gctttttgga aatgttctaa agaatcgttt ttgtcatcat
     2161 cttccattaa tctagcataa gtaacatatc catcagctaa ccaaaacatt gctgctacat
     2221 ttccattttc tgctgattta agagcccaaa cttcagcttg attttcatct gaatcaacac
     2281 catgaccata tagataaatc tcggctaatt ctaactgagc agaagtatct ccattttctg
     2341 ctttcgctaa taaatcatta gaaaaaacag gttctgcaaa tgtttccatt gaaaggcaaa
     2401 ctcctaaagc cataaaaata tttgttaatt tattcaattt ttaaccttta aactaaactg
     2461 ttaggcttgt atatttttat atcataccta aaagaaaact ctaaaattag agtatgtctt
     2521 atctctattt aacataatgg cgattatacg aagtacactt gtatattaat ataaatatca
     2581 actacttaat aaaaatagaa aaatcccaaa atgcccaaaa agccgacttt gaaacaagtc
     2641 ggctttttta tgagcagttt tgatacttct tgccaataat tttgactaaa aattacacga
     2701 atctggttga gtgatacaat tttgtgatac attattgtat atacaaattt gaatgttttt
     2761 tatggaacag tattttgaat gggatgaggc taagaatcga aagaatcaga aaaagcatga
     2821 tatctctttc gaaactgcaa gccttgtttt tgaagatcca ttacggatct caatccaaga
     2881 cagacatacc aatggtgaag aacgttggca aaccattgga cgagttaaag gcgtactaat
     2941 gttattggtg gctcacacca tttttgatga agatgactgt gaaatcatcc gaatcattag
     3001 tgcaaggcaa gtcactaaag cggagcgaaa caaatatgag catggttaga tactcacaca
     3061 aagagctgaa tgagaaattt ggtgagaagc aagatgctga aattcaacgc ttgcttgcta
     3121 aaggcacggt tcctgatgat cagctagatc tatcagatat tcctgaaatt acagactgga
     3181 gcaatgctgt acgccaaaat caattttacc gcccagtaaa gcaacaaact tcaattcggt
     3241 tagatgctga tgtacttgct tggttcaaag cacaaggcaa aggttatcaa acacgtatga
     3301 atgaaatttt acgagatgct atgctaaaag aattaaaaaa tcatcaataa gaatgggagc
     3361 ttaggctccc atttttgact tcatttttag gggggattac cattcaaaaa tttaaatagt
     3421 agattttata aatttttaag tgttatatta taatataaca ttaaatttgg ttgttttatg
     3481 ctaaataaaa gtaaactatt tttagcttta attactttag gggcctcgaa aattttattg
     3541 gcagctgaag ggcctgttac taccttaaat acaatagtat taactgcaca atctgatgag
     3601 ttagggtctg agctgttagg caaaagtcta aatgttagta atcagtttat tgatactagt
     3661 aaacttaaac agcgttcgac aactctaggg gatgccttgg gtacggaatt aggaattcat
     3721 tctaatcaat atggaggtgg agcatctact cctattattc gtgggcagga aggaaaacgt
     3781 attaaagttt tacagaataa tgctgacgtg ttggatatgt ctaatatgtc cccagatcac
     3841 gctgtcacag ttgaaccaag tttagctaag tctattgaaa ttatacgtgg tgcaagtacc
     3901 cttttgtata gttcaaatag tgcggcaggg gtagtcaatg tcattgacta taaaattcca
     3961 acccaaatgc ctcaagacgg attagagggt aatactacat tacgttttaa tactggcagt
     4021 aatgagaagt tgaccacagc aggtgtaaca gttggcttaa gcccacatgt agctttacgg
     4081 gcagaagggt tatatcgtaa tgctggtaac tataaaactc ctcattacca gtctagctct
     4141 tataattctt tagaagatct tgaaaaccaa aacagtattt ataaaaattt aaaatattta
     4201 ccagaaagct gggcagagtc gagagtaggt acgcttggat tatcctggat agacgataat
     4261 acttatttag gagtttctta tacacatcgt catgatgaat atggtttacc agcccatagc
     4321 catttatatg aaggttgtgg tgcctctgca atcggtatcg attcacgaat tagtggatta
     4381 aaaaattatc ttttatatta tcctcaattg atggatgagc aagatattaa ttacataaat
     4441 ccacgtcctg attgtcatca gcataatcat attcatgaaa ctaatttctc tcataatgct
     4501 ccttatattg atttaaatac tcgacgttat gatgtgcgtg gagaattcac tcaaccattt
     4561 accggtattg acaaaattcg tacctcattg agttatatag attattttca caatgaactc
     4621 gagggagata agattactaa cttttttaaa aatacaggaa aagtaggccg catagagtta
     4681 tcacatcagc cattagggga attaaccggt atcttggggc tacaatattt ggaacaggat
     4741 aatagtgctt tatcaccagt ccattctcaa gaaggccata ccacctatct agataatcag
     4801 cagctattaa atcgaaatgt cactaaaaac tttagtgtat ttggcctaga aaaatataac
     4861 tggaacgact ttactttcga actaggtgct cgtatagaaa agcagaaagt tagtatggat
     4921 tatgatatag aaaaaattaa agattcaatg aaaccttggc ctaataaata taacagtcct
     4981 tatgtagaaa aaaataataa aatcagggct caaaatttaa aatctatttt agaagctgtt
     5041 caacctaaca aagaaacagc tttttcttat gcaggtacag tccactggag atttgctcca
     5101 aattatatct tatcattaac aggtacacat caagaaagat tacctaatgc acaagaaatg
     5161 tatactcatg gtatgcatct cgctactaat tcttttgaaa taggtaaccg ctttctaaga
     5221 aaagagaaat ccaataattt agaaatcagt ctggcctata aggatgattt acttgattat
     5281 caaatcagta cttactatta tgattttgat aactatatat atttacagac actcaatgaa
     5341 gttttaggaa ctacaaaagt tagagatcag catacactac gtattaatca ttatagccag
     5401 tctgctgcta atttttatgg attagagggc aacatagggt atcaatttaa ttctgtttat
     5461 cacggctctc tttttggaga ttatgtgaaa ggccgtttaa caaatttacc tgatgctgta
     5521 attgcatacg atatctggaa tagagaaccg actttagctc cccaaaagga tcgttacact
     5581 cctagattac ctcctgctcg tttgggtaca cgactaaaag ctgattttga tgaatcgctt
     5641 aaaggtgaaa tagaatatta tcgagtcttt aaacaagata atatttctaa atttgagcag
     5701 gttacatcag gttataatat gcttaatatg actttagcat ataaaaataa gctatctcac
     5761 acagaatatg atttattctt taaagcgaat aatttacttg atcagaaagt ctatgcccat
     5821 gaaacatttt tgccatatat tccccaaatt ggaagaaatt ttagtttagg cttaaatctt
     5881 aatttctaga taagatactg taatcttcta agaaaatttc ttagaagatt tttcttataa
     5941 aatgattaga ttaataatta aggtaagcgt ccgctgaatc ccatacctat tttttaattc
     6001 gatttaggtt tccagcagtg tggcagtaac tcttcaatct gggtcacttt atgtgtcggc
     6061 agccttttca gcactgaata gccaccgatt ttgtagacac cttttagcta gataaaaagc
     6121 aatttttttg atctttaaag aatgaggata taagctgttt aaaacttaat tctaattggt
     6181 taataggtga aaaatgaaat tagcagaagc tctcttactt cgaagtgatc aacaaaaaaa
     6241 aattatttca ctaaaacaac gtatcaatgc aaatgtattg gtacaagatg gtgatcaacc
     6301 atctgaagat ccaaatgaac tactaaaaca agtattttct ttaattcaag aatttcaaaa
     6361 attaagttat gcaattcatg aaactaatgc tttaacaaaa ttaaatgatg gccgttctct
     6421 acttgcttta ttgactttgc gtgatgaatt tgtagaacag cacaaaaccc taactgcagc
     6481 aataagtaat acatctcgtg aatctgatcg ttatagtacg cgggaaataa agtggcataa
     6541 agttattcca gtttcatcat tacaaaaaca agctgatgat attagtctta aattacgtga
     6601 tttaaacgtg ttaattcagt cgactaattg gaaaattgat ttgattgaag cctaatagtc
     6661 ctttagatat aatcaagttg aacatactgg gcgagtacaa gatcccttac ttccacaaca
     6721 taggattgta aataaagtgt agtggcttgc aaagcaagtg gataagctta aaattttaag
     6781 tacaaatcaa tccatcactt ggcataactc aaatctttgt gcaaacaaag ttacacgtta
     6841 cttataacta ccatgtactg acagtatgtt cacctcaatt actaaaaata aagaaatgaa
     6901 caagcatcag ctcaactaca tttctatttt taattcagtt cggcatttaa cataaaatct
     6961 cttatgcgaa attacatacc aactttaacc acattgtgga caggttggag tttagagata
     7021 agaaaaattt ctagagcatt tgctaaataa gccctatcac gttcaaagcc tatcgtaatg
     7081 atgggcgaat tcaaacatga ggtgcgacag tttcaaaagc catatgataa tcaacaagct
     7141 gagcaaattt ctctaatggt gtaagccaat ctaacgcctt tctaggacga gtattcagtg
     7201 acatggcaac ttgattaaat aatgctgatc tgcctgattt aaatcaatcc ctttaggtaa
     7261 atattgccta attaaaccat tcatattttc gcatgtgcct ttttgccagg gtgaatgtgg
     7321 gtcacagaaa tatacatcta tgcctaaatc ttcttcgagt attttatgtt ctgacatctc
     7381 acgtccacgg tcataggtca acgttttacg cagttctgca ggtaaatatt tcagagcttc
     7441 agttaaagcc ttgcgcactg attctgcctt tgcatcaggt aatgttgcca agatacagag
     7501 ccgtgtattt cgttcaataa gtgttgctat cgaactttta ttgtctttac ctttaattaa
     7561 atcagcttcc caatgacccg gtatttttct ttcttgaact tcggctgggc gctcatgaat
     7621 agttttaata tcctgtaata tagaatcttt tttaggttca ccgttagctt ttcgcttttt
     7681 attttcatga cgtagacagg ataataagtc ttttttcaac tcacccttgg gtaatgctcg
     7741 tatcgttgaa taaatcgttg tatggcttac attcattgtt tgatccaaat caggaaatgt
     7801 ctttaaacgc tttgctattt gctgaggaga ccataaacaa cggatcgctt caacaataaa
     7861 tttccagagg attgaatcga ttttgagttt tctgtgacca cgtctacgtc tagcaaaagt
     7921 gttatcagaa gcatattgag cttgataaac gtcattgatg ctatttcttt taagctcacg
     7981 atagatcgta ctaggatgtc ttttaataag ttcagcaaat tttctggctg aaaagccttc
     8041 ttttcttgac tcaagcatta atgcagtacg atcttcaaag ttaagatgat ggtatgacaa
     8101 ttttatatac tccataaacc ctttaaatta attaggtggt ttatgtcgca cttcaagttt
     8161 tactctgcca tggatccagc atcttttaat cttggatcta tcgcaatata ttcagtttta
     8221 tcttcattcc atcggcaata tttcattacc ctggcgctta agccattacc acgataacgg
     8281 ccagcacctc tagaagtgcc aacaccgata taagtcacta aatccccttt gataaacgca
     8341 tatacacctt gtttatcgta attaggaatt ttcccaaaat taaaatgtac tggtgaccat
     8401 tcaggaggct ttcctaatag atctacattc cagtgatgtt caaagaataa ttctgtatta
     8461 cgtattaact cattaagtgt tgaatcttcc actactcatc tctcaaaatt tatagattaa
     8521 atagctttcg gtcacgctca tccgttaaat acttatccat ctgagcaatt agtttagctc
     8581 tggcttgttc atcatcttca gtgattttta acatacagga tccaattaaa atcttgcgtc
     8641 tggtatcgtc ctttcgtgcc tgttctttct ctttggtacg ctctcttgcc agtgctgcct
     8701 gtctttgagc ctttaactgc tttagctttt cttcctgtgc cttgattttg ctgtctagtg
     8761 tttcagtaac gctcattaat ccaataccat cattctggga aaccatccct aacataagcg
     8821 cacttgaata tgcattcaag gtgctatatg tatagtaatg ttttcctcga agagcgcact
     8881 tatgcaaact atgtttgcaa gtccgattgg ggtctcccca ataccccgac aaccgtattc
     8941 acggttgtat ttcactacgt gaaaatttaa aagcgaaaaa tagacatggc gatttaccac
     9001 ttttcagtca aaaccgtagc acgatcagca gggcgttccg ccactgctgc gattgcctat
     9061 cgggcaggtg aaaagatcta ttgtgaacgt gaaggccgag aacatgacta cagccgaaaa
     9121 accggtgtgg aatacaaaga aatttattta ccagaaggcg caccagagca tttaaagaat
     9181 cgagaaaaac tctggaacga agttgaacaa cgggagaccc gaaaaaactc gactgtagcc
     9241 agggaatttg aaattgcctt tccaagtgaa ctgaaccagg aacaacgtct ggccatgctc
     9301 gaagaattat gtgccagcat cgtggaaaga catcaagtag cagtcgatgc ctgtattcat
     9361 gcaccgcata ccggatccgg cagtgatgag cgcaattatc acgcccatat cctaatgagc
     9421 acacgtaaac ttacgccaga aggttttaca gagaaaaccc gggaactcga tcagaaacac
     9481 agtggagaaa tcgatcactg gcgtgaacac tttgccgata tctgcaatgt gcacctgaat
     9541 ctggccggat ccacagcccg ggtcgaccac cgcagctaca aagaccagga gaatggcctg
     9601 gaagccaccc tgcacgaagg accgaaagtc accgaactac gtagaagagg catcgaaacc
     9661 gaaatcagcc gaagcaatga tgaaatcaaa cagagaaatc aggctcagtt gcaatacgac
     9721 aaaaatatgg acgttctaat tgctgaaaat gagataaaac tcagcacact gaaaacagaa
     9781 cagcagatcc aaatcaagaa tagcgctaaa acgccaccaa ttgacgaaaa atccctgttt
     9841 gaggaaaaac agaggggaac ccttggcaaa gtgctaaaac gcgaaatcag cgcaaaagac
     9901 gcgaatctag acctggattt tatgcagcgc aatctcaaac aagccgaaat aaacctgacc
     9961 aagcaccaca aacatcaaaa cgagttcaat cagcagcttg cccaggagat cgtgaaaagc
    10021 ggactcaagc aaagtcatga caaattgcaa agcctggtcg atcaacacaa cgaattaacc
    10081 cagaacaaac ccttactgtt tggaaaaaaa gcctgggaag cccaacgtga tgagatctac
    10141 caggaacaca aaaagttgaa aggtcagcat gaacaccaga aaaaacatgg tgtgaaggat
    10201 ttattggaga atgaaaagtt caaagaacat gcctggaaga aatatcaaaa acagcatccg
    10261 gagaaagcca agcaatacca gaccctctac cagtcctaca agatcgttaa aaaatgtgtg
    10321 gatgagatta aagcagaaca acagatgaaa atcagacaag aacaacaact caaagcacag
    10381 caacatgcgc ctaaaatgaa atctcgtggc atgagtcgtt aaacagagcg agtgtctacg
    10441 agcgaactga gaaaatttgc ccatcccttt ccctgattac aagctcccta tccatcaatc
    10501 acctgcatac cagagcatcc cgcatgggtg cgaactttcc aagtttgatt cctaaatcga
    10561 gcgtagcgag aaaaaaagct catgagcgaa gcgaattccg agttgctttt gatcttgctt
    10621 ttgctttttc tcaaattcac gaaaatatga accaaaaatt gccccgacga atcgagcgaa
    10681 agcgagattc aatagagttt gagcgaacga agtgaagcga aaaccaaggg caatttttca
    10741 ctccttgggc ttttaattat tttaaagttt ttaaatgctt ttagatatgc tgaaagcctt
    10801 tgttcacaat gcttttaagg tttatatgac accgtttacc ttgcaatatg acaccgttta
    10861 ccttgcaata tgacaccgtt taccttgcaa tatgacaccg tttaccttgc ttatagcact
    10921 aaaattaatt attgtgtcat agcataataa ttaatactgg tgcttc
//
