LOCUS       NZ_CP096832             3751 bp    DNA     circular CON 10-JUN-2024
DEFINITION  Escherichia coli strain PM4-5 plasmid pPM4-5-3k, complete sequence.
ACCESSION   NZ_CP096832
VERSION     NZ_CP096832.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27593794
            Assembly: GCF_023272775.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Li,Y. and Li,R.
  TITLE     tet(X4)-positive E. coli isolated from slaughterhouses are highly
            relevant to human isolated
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Li,Y. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2022) College of Veterinary Medicine, Yangzhou
            University, 48 Wenhui East Road, YangZhou, JiangSu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: QitanTech Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023272775.1-RS_2024_06_10
            Annotation Date                   :: 06/10/2024 04:29:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,188
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,880
            CDSs (with protein)               :: 4,880
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 53 of 190
            Pseudo Genes (incomplete)         :: 129 of 190
            Pseudo Genes (internal stop)      :: 39 of 190
            Pseudo Genes (multiple problems)  :: 28 of 190
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096832.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PM4-5"
                     /isolation_source="cecum"
                     /db_xref="taxon:562"
                     /plasmid="pPM4-5-3k"
                     /geo_loc_name="China"
                     /lat_lon="32.15 N 119.01 E"
                     /collection_date="2020"
                     /collected_by="Yangzgou University"
     gene            join(3344..3751,1..519)
                     /locus_tag="MYF75_RS00610"
                     /old_locus_tag="MYF75_00610"
     CDS             join(3344..3751,1..519)
                     /locus_tag="MYF75_RS00610"
                     /old_locus_tag="MYF75_00610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_014342223.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGYDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            522..1055
                     /locus_tag="MYF75_RS00615"
                     /old_locus_tag="MYF75_00615"
     CDS             522..1055
                     /locus_tag="MYF75_RS00615"
                     /old_locus_tag="MYF75_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168772.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            1489..2508
                     /locus_tag="MYF75_RS00620"
                     /old_locus_tag="MYF75_00620"
     CDS             1489..2508
                     /locus_tag="MYF75_RS00620"
                     /old_locus_tag="MYF75_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271364.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(2769..3167)
                     /locus_tag="MYF75_RS00625"
                     /old_locus_tag="MYF75_00625"
     CDS             complement(2769..3167)
                     /locus_tag="MYF75_RS00625"
                     /old_locus_tag="MYF75_00625"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222916.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     Typing          1490..2264
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.907"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          3345..3751
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.529"
                     /coverage="100.0"
                     /evalue="1.93e-84"
ORIGIN
        1 acaccggaac agtatttccg gcgctataac gccaggtatc ccgagcgggg aggtgcgaaa
       61 aaggattccg gaagcctgac tctcactcag caaaaagagc agttgcggga attgcgaaaa
      121 cgctgggagg tgaagcacaa cgaacatatg agaaagcatg gctttgaaag gggcttcatt
      181 gactgccgga cgctgaaaga acagggcatt gaacgacgtc ccgaagtgca tctgggtttt
      241 gaagctgccg gacggcttga tgatgcccag cgtcatgaca tcatgcaaaa acggtctgaa
      301 ccggactatt cccgccatct tgaacaggcg cgaacggtcg ggtatgacat cagggaatgg
      361 ctggacactc cggttaagca acatgaactg gtcagaaggc tgcaacatga tgttgcgcgg
      421 gagcatggct ttgacatgag agacagagtg agcagacaga accgaactga acctgaacgg
      481 gttaaatcac cgtcacgtgg attttctatg ggattctgaa catggccagc acagaagaaa
      541 aattactcac gctcctgtta tcggtggagc atctgcaaca taccgcaatg gagcaacaga
      601 aagctgttgc tgattctctg gctgtggcaa gggaaacact tcgcggatat cgggatattt
      661 ctgacggtgt ggcaaaacag gtcagggatg aagtcagaaa cgaggtgcag aagctggatg
      721 ttgcagggct gataggtgag cgcatcagcc aggattttca gcggctggaa gagtcagtcg
      781 caaacatgaa ggcaaacgcg gatgccctga acagggatat tgcgacaatt cacagcaata
      841 tcgtgcagga atacaacacc cttaaagggt atagctggaa atggctgggt ggggtgtcag
      901 tggcttttct ggttatgttc tgtgtgcttg gatggggaat gaaaacaatg ctcgacagga
      961 actacaacgc cacagcggcg gtgtatcaga aagttgtgac actggaagat aaactggcgg
     1021 agaaacctgt cacgggcaag agccggaaaa actgacgcgc ttcgcttgtc caacgcctca
     1081 cgacccgcga aaaaattccg cgtcccgttc accgttttct gatgagaaag tttgctgttt
     1141 tgacccgttc gcccttctga acgctcgaaa cagatcgcgc attgctttcg tgaatagtta
     1201 tgcagcctcc taaaaacaat tctgccgcgt ttttctggtt tggcctggtg tttccttgtc
     1261 tttttgcgtt ttttgcgcca gaaagcgact gagggcgttt taaggggtac gtacaacggg
     1321 agttatggta aatggatcgg ttttgcggga aggctcgaca ggttttgttg ttgggggtag
     1381 tgtaagcgac tgaaaaacaa acgcccctga aattcgtgtc tctccgccaa gattgtcacg
     1441 agaatacagg ggcgaggtcg gtcgataacc tggaaggaat cgtaacacat gagcgccgcg
     1501 cttcaatact tcgaagaaaa tttaccccac cgcccctatc acacggatga tctcgctttc
     1561 ggccttcgca tctccggcaa agggcgtgcg cttcttgcgc ggtacatcca gcagaaccag
     1621 cctcatgcgc agttctggct ggtttttgac gttgaccgcg agggggctgc gattgactgg
     1681 agcgaccgga acgcccccgc gccgaacatc accgttaaaa atcctgtgaa cggacacgct
     1741 cacctgctct acgcactcaa catcgccgtg agaaccgcgc ctgatgcctc ggttaaggcg
     1801 ctgaaatatg ccgcagcggt tgaacgttcg ctgtgtgaga aactttgtgc tgatgtgaac
     1861 tacagcggcc tgatttgcaa aaatccgttc cacctggaat ggcaggtgat ggagtggcgc
     1921 gaggacgcct ataccctcga tgaactggca gattatctcg atttgagcgc ctcagagcgt
     1981 cgtagcatcg ataaacatta cgggatgggg cgaaactgcc acctgttcga aatgacgcgc
     2041 aaatgggctt acagggcgat tcgtcagggc tggccagcgt tctcacagtg gcttgaagcg
     2101 gtgatccagc gtgtcgaaat gtacaatgca tcgcttcccg ttccgctttc actggctgaa
     2161 tgtcgggcta ttggcaagag cattgcgaaa tacacgcaca ggaacttcac gccggaaact
     2221 ttcgcacagt atgtggctga tacgcacacg ccagaaattc aggcagcacg cggtcgcaag
     2281 ggcgggaagg ccaattcgtc agaaaatcag tcggacaaag ggaaaaaaag cgccgctgtt
     2341 cggtggactg ctaacgacga caaacgtcgt agggcgcttg atatgtatat cttgggagcg
     2401 tcaacggaag atattgcagt ggcggttggt gttagcagcc ggactattcg acgctggatg
     2461 gataattctg gagaatggct aaccaagaaa caaataatta aatgttagtt aacaaagaag
     2521 caacactacc ccccgttttt tgttaatggg atgttaaatt cgcggacatg aacaagccta
     2581 tatcagataa cagcgccttt cggcgtcttt ttgagcagcg ggctttccgg cggttttctc
     2641 ctctcagccc agcaatggtg cggcttaccg catgattgag gtggtcgtgc tcgccgcagt
     2701 ctcatgaccg agcgtagcga gcgaatgagc gaggaagcac cagggaaccg gaacacggat
     2761 ggaggcactt acgcgtctgg gtttggggat gtctccagat cgtacttaat ttccttaagt
     2821 gcctgtaata tcctgtcttt tttatagcat agctcgcctg tttttgtgtc taccagtcct
     2881 gcttcgatca gtagcccagc cgcgagaaag agtcggtgat tgcgttctcg tcgttgtttt
     2941 tttccctcga ttcgtttttg gaccgcctgt tttcgttcgc tctcctgtgc ctgtaagtcg
     3001 tttttttccg ctgttgccag tacgtggagg agtttgcgtt cctgttctgt gagtcctgct
     3061 tcgttgtcga gcaggtcaat aatctgtctc tgtcgtgcgg tctgttctcc gagcttcctg
     3121 atgaatccga tccgtttctt cagccattcg atgttgctga tgcgcatgtt cgtggtgctc
     3181 cctggtgttt ttttgcggtg tgttgtggtc gtgcagtata tcgcttgcgg tcggcgggtg
     3241 cagggggtat cttccgtgct aattcgtaaa attagaagga agcgcactta tatgtttctc
     3301 tccttcgtcg agaaacagtg cgctcctgcg gagggctgaa accgtggcaa cctatcacct
     3361 cagcgttaaa tttggtggca aggggcaggc tgcaaaccac gccgattaca tcgagcgcaa
     3421 agagaaatac cgcgaccgtc aggatttgga atattccgca cacggaaaca tgccggaatg
     3481 ggcgcgagat aatccctcac atttctggca ggctgctgac cagtttgaac gtgcgaacgg
     3541 ttcgacctat cgggaacttg aaattgcatt accccgtgaa ctgacagcgg aacagcgcct
     3601 tgaactggtg caggattttg tacgtcagga agctggcgaa cggcacgcct ggagttttgc
     3661 cattcacaat ccgaaagcga gcattgacgg cggcgaacag cctcatgcgc atatcatgat
     3721 gagccagcgt gtgaatgatg gcattgaacg t
//
