LOCUS       NZ_CP096820            11805 bp    DNA     circular CON 05-JUN-2024
DEFINITION  Acinetobacter baumannii strain Mu1984 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP096820
VERSION     NZ_CP096820.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27957725
            Assembly: GCF_030168245.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11805)
  AUTHORS   Choby,J.E. and Weiss,D.S.
  TITLE     Heteroresistance via beta-lactamase gene amplification threatens the
            beta-lactam pipeline
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11805)
  AUTHORS   Choby,J.E. and Weiss,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2022) Division of Infectious Disease, Emory
            University School of Medicine, 954 Gatewood Road Room 2052, Atlanta,
            GA 30329, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030168245.1-RS_2024_06_05
            Annotation Date                   :: 06/05/2024 04:33:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,885
            CDSs (total)                      :: 3,790
            Genes (coding)                    :: 3,693
            CDSs (with protein)               :: 3,693
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 63 of 97
            Pseudo Genes (internal stop)      :: 10 of 97
            Pseudo Genes (multiple problems)  :: 6 of 97
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096820.1.
            Bacteria and source DNA available from David S Weiss, Emory
            University.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11805
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="Mu1984"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA:Georga, Atlanta"
                     /lat_lon="33.7911 N 84.3233 W"
                     /collection_date="2012/2015"
                     /collected_by="Georgia Emerging Infections Program"
     gene            1..951
                     /gene="repM"
                     /locus_tag="M1R67_RS19405"
                     /old_locus_tag="M1R67_19395"
     CDS             1..951
                     /gene="repM"
                     /locus_tag="M1R67_RS19405"
                     /old_locus_tag="M1R67_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001208778.1"
                     /translation="MRELVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHAEGYINQFGVHRNTAYQALKDACNDLFARQFSYQKINERGNIENYRSRWVSEIGY
                     VDNEAVVKLIFAPAIVPLITRLEEHFTKYELQQVSNLSSAYAVRLYELLIAWRSTGSTP
                     VIELSDFRQRIGVLDTEYKRMERFKTSVLELAIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDNPPIERDPNTLDLFTKMTDAQRHMFANKLSELPEMGRYSQGTESYPQFAV
                     RIAEMLQDPDRIKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            944..1519
                     /locus_tag="M1R67_RS19410"
                     /old_locus_tag="M1R67_19400"
     CDS             944..1519
                     /locus_tag="M1R67_RS19410"
                     /old_locus_tag="M1R67_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096619.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSRQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            1539..1682
                     /locus_tag="M1R67_RS19415"
                     /old_locus_tag="M1R67_19405"
     CDS             1539..1682
                     /locus_tag="M1R67_RS19415"
                     /old_locus_tag="M1R67_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            1988..2263
                     /locus_tag="M1R67_RS19420"
                     /old_locus_tag="M1R67_19410"
     CDS             1988..2263
                     /locus_tag="M1R67_RS19420"
                     /old_locus_tag="M1R67_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="WP_001133626.1"
                     /translation="MPNQVEDKKKILTRVRRIKGQAEAIEKALEDNVECGAILQQICSV
                     RGAINGLMNEMLEVHLKDTLVSGETTEQQRKEELAEIAKILKSYLK"
                     /name="metal/formaldehyde-sensitive transcriptional
                     repressor"
     gene            2285..3394
                     /locus_tag="M1R67_RS19425"
                     /old_locus_tag="M1R67_19415"
     CDS             2285..3394
                     /locus_tag="M1R67_RS19425"
                     /old_locus_tag="M1R67_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995413.1"
                     /GO_function="GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA]; GO:0051903 -
                     S-(hydroxymethyl)glutathione dehydrogenase (NAD(P)+)
                     activity [Evidence IEA]"
                     /GO_process="GO:0006113 - fermentation [Evidence IEA];
                     GO:0009404 - toxin metabolic process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="WP_000842148.1"
                     /translation="MKSRAAVAFGPGQPLQIVEVDVAPPKKGEVLIKISHTGVCHTDAF
                     TLSGDDPEGVFPAILGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECVFCKSGKSN
                     LCISVRETQGRGVMPDGTTRFSYNGQPIYHYMGCSTFSEYTVVAEVSLAKINPEANHEH
                     VCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGGIGLAVVQAARQAKAGRIIVVDMNPD
                     KFALAKEFGATDFLNPKDYDKPIQQVIVEMTGWGVDHSFECIGNVNVMRAALESAHRGW
                     GQSVIIGVAGAGQEISTRPFQLVTGRKWLGSAFGGVKGRTQLPGMVEDAMKGKIQLEPF
                     VTHTMGLDQINEAFDLMHEGKSIRTVIHY"
                     /name="S-(hydroxymethyl)glutathione dehydrogenase/class III
                     alcohol dehydrogenase"
     gene            3447..3845
                     /locus_tag="M1R67_RS19430"
                     /old_locus_tag="M1R67_19420"
     CDS             3447..3845
                     /locus_tag="M1R67_RS19430"
                     /old_locus_tag="M1R67_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="WP_000235176.1"
                     /translation="MATPVISGNDIFSHVFLGAVDLKKSVVFYDAALGALGIKNLGPFG
                     NDWVLYGRDKPAFMITRPSNGAEPTSNGVTVGFAAATPAEVNAFHAAGLAAGGTDEGQP
                     GPRGHLPGAYAAYLRDPAGNKVCAYTFV"
                     /name="VOC family protein"
     gene            4319..5152
                     /gene="fghA"
                     /locus_tag="M1R67_RS19435"
                     /old_locus_tag="M1R67_19425"
     CDS             4319..5152
                     /gene="fghA"
                     /locus_tag="M1R67_RS19435"
                     /old_locus_tag="M1R67_19425"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439570.1"
                     /GO_function="GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="WP_000439570.1"
                     /translation="MERIEHHASFEGWQDVFQHESTVLGCMMRFGIYLPPQAEHEKLPV
                     LYWLSGLTCTEQNFITKAAVQRYAAEHGIIVVAPDTSPRGESVADDAAYDLGQGAGFYV
                     DATELPWSDHYQMYSYVVDELPALIEANFPASSRRSISGHSMGGHGALVIALRNPGRYA
                     SVSAFSPIVAPTQVPWGQKALAAYLGEKNLEAWKQYDTVELIRVAQERLPLLVDQGLSD
                     EFLEHQLRPQLLRTACEQVDHPLTLNLRPGHDHSYYFISTYLSEHMAHHAAALNR"
                     /name="fghA"
     gene            complement(5908..6180)
                     /gene="nadS"
                     /locus_tag="M1R67_RS19440"
                     /old_locus_tag="M1R67_19430"
     CDS             complement(5908..6180)
                     /gene="nadS"
                     /locus_tag="M1R67_RS19440"
                     /old_locus_tag="M1R67_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_000369780.1"
                     /translation="MDNNLFDDLVASIKEAGAIKRKEIKASRVTELELPDIKEVREKTG
                     LTQAEFAARLHISARTLQNWEQGRRYPTGPAATLIRILDAHPTLI"
                     /name="nadS"
     gene            complement(6173..6493)
                     /locus_tag="M1R67_RS19445"
                     /old_locus_tag="M1R67_19435"
     CDS             complement(6173..6493)
                     /locus_tag="M1R67_RS19445"
                     /old_locus_tag="M1R67_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000897309.1"
                     /translation="MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGI
                     RKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAYPKSVKDNLTDKETSILRQLVKEQF
                     HG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6680..6877)
                     /locus_tag="M1R67_RS19450"
                     /old_locus_tag="M1R67_19440"
     CDS             complement(6680..6877)
                     /locus_tag="M1R67_RS19450"
                     /old_locus_tag="M1R67_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000476222.1"
                     /translation="MFKQTLSVKDQFGVKHAIQATVDHEFANTQSHLNIKHITVDGEDI
                     RPSFEMLFQSTLSGKIFKII"
                     /name="hypothetical protein"
     gene            complement(6944..7171)
                     /locus_tag="M1R67_RS19455"
                     /old_locus_tag="M1R67_19445"
     CDS             complement(6944..7171)
                     /locus_tag="M1R67_RS19455"
                     /old_locus_tag="M1R67_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000921865.1"
                     /translation="MLKVNDLIAKSKNGTEIIVSLIPLHKMQNTRQGLKQIEVGKRVLL
                     ESGIEVDLNLDGRTFYTSLNQLFKLNHKVA"
                     /name="hypothetical protein"
     gene            complement(7270..7485)
                     /locus_tag="M1R67_RS19460"
                     /old_locus_tag="M1R67_19450"
     CDS             complement(7270..7485)
                     /locus_tag="M1R67_RS19460"
                     /old_locus_tag="M1R67_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004693381.1"
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]; GO:0003723 - RNA binding [Evidence IEA]; GO:0001072 -
                     transcription antitermination factor activity, RNA binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="WP_000071892.1"
                     /translation="MSNSNVVKGTVKWFNETKGFGFIQQESGPDVFAHFSEIASSGFKT
                     LFEGQQVEFSVAQGQKGPNAVNIVAI"
                     /name="cold-shock protein"
     gene            complement(7930..8898)
                     /locus_tag="M1R67_RS19465"
                     /old_locus_tag="M1R67_19455"
     CDS             complement(7930..8898)
                     /locus_tag="M1R67_RS19465"
                     /old_locus_tag="M1R67_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611607.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_012308653.1"
                     /translation="MLESRKEGFSARKFAELIKRHPSTIYRELKRNSINDVYQAQYASD
                     NTFARRRRGHRKLKIDSILWKFIVEAIRCLWSPQQIAKRLKTFPDLDQTMNVSHTTIYS
                     TIRALPKGELKKDLLSCLRHENKKRKANGEPKKDSILQDIKTIHERPAEVQERKIPGHW
                     EADLIKGKDNKSSIATLIERNTRLCILATLPDAKAESVRKALTEALKYLPAELRKTLTY
                     DRGREMSEHKILEEDLGIDVYFCDPHSPWQKGTCENMNGLIRQYLPKGIDLNQADQHYL
                     NQVAMSLNTRPRKALDWLTPLEKFAQLVDYHMAFETVAPHV"
                     /name="IS30 family transposase"
     gene            complement(9000..9332)
                     /locus_tag="M1R67_RS19470"
                     /old_locus_tag="M1R67_19460"
     CDS             complement(9000..9332)
                     /locus_tag="M1R67_RS19470"
                     /old_locus_tag="M1R67_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000390243.1"
                     /translation="MEDSTLNELIRNTELFFEHHWNVDLLGKPPEWSPVHFNFGKIPNY
                     DKQGVYAFIKGDLVTYIGVGTSRGAGRYRGNGLSARVMKYCRWNEDKTEYIAIDPRLKD
                     AGSMAE"
                     /name="hypothetical protein"
     gene            complement(9350..9616)
                     /locus_tag="M1R67_RS19475"
                     /old_locus_tag="M1R67_19465"
     CDS             complement(9350..9616)
                     /locus_tag="M1R67_RS19475"
                     /old_locus_tag="M1R67_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050441450.1"
                     /translation="MSVTETLDSKIKAQEEKLKQLKAQRQAALARERTKEKEQARKDDT
                     RRKILIGSCMLKITEDDEQARAKLIAQMDKYLTDERDRKLFNL"
                     /name="hypothetical protein"
     gene            9826..11261
                     /gene="mobQ"
                     /locus_tag="M1R67_RS19480"
                     /old_locus_tag="M1R67_19470"
                     /pseudo=""
     CDS             9826..11261
                     /gene="mobQ"
                     /locus_tag="M1R67_RS19480"
                     /old_locus_tag="M1R67_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248576.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /name="mobQ"
     Typing          2..951
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9827..10794
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.808"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          10792..11258
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="81.41"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagagaat tagtggtaaa agacaatgcc ttaatcaatg caagctataa cttagattta
       61 gtagaacaac gtttaatttt attggccatt gttgaggcaa gagaaagcgg gaaaggtatt
      121 aatgcgaatg atccccttga agtacatgca gaaggctata tcaatcaatt tggcgtacat
      181 cgcaatacgg cttatcaagc attaaaagat gcctgtaatg atttatttgc aagacaattt
      241 agctatcaaa aaataaatga acgagggaat attgagaact atagatcccg ttgggttagt
      301 gaaattggat atgtagataa tgaagcagtg gttaaactta tctttgcccc agccatagtt
      361 cccttaatta cacgcttaga agagcatttc actaaatacg aattgcagca agttagtaat
      421 ctcagcagtg cttatgctgt tcgcttatat gaattattaa ttgcttggag aagtactggc
      481 tctactcctg ttatagagct aagtgatttc cgtcaaagaa ttggcgtact cgatacagag
      541 tacaagcgta tggaacgctt taaaactagt gtacttgagc ttgctattaa acaaattaac
      601 gaacatacag atatcactgt gaagtatgag caacacaaaa gaggtcgatc aatttcagga
      661 ttctctttta cttttaaaca gaaaaagaag gacaacccac cgatagaaag agatccgaat
      721 acgttagatc tctttacaaa gatgactgat gcacaacgcc atatgtttgc aaataaactt
      781 tctgaacttc ctgaaatggg tcgttattcc cagggaactg aaagctatcc gcaatttgct
      841 gttcgtattg ctgagatgct gcaagaccct gaccgaataa aagaactata cccataccta
      901 aaaaaagtgg gatatatgcc atcaaataaa aaggacaccg taaatggcta agttatcact
      961 aagtgaagta tctaaaaaat tccatgtgga tagatcaacc atttacagag ctgtacgtaa
     1021 tggacgttta tcacgctcca gtgatggaca attcgatcta gcagaggtca tccgatgctt
     1081 tggagaacct gagcaaacat ctcaaaaaat tgaatcatct aggcaagaag gtgatgaatc
     1141 tacaaaaaaa cttattgccc atttagaaaa tgaagtcaaa aaataccaag aacgtgaaga
     1201 acggttaatg caacaaattg accgtatgca aacactcatt gagctgaaaa gtgttgcacc
     1261 tgccacagca gcaccacacc aagatgctac ggcatgcgac accaagatgc cacagcatgc
     1321 gacaacacaa caagacactg ataacaaaaa gaataatgaa ttaaatattg cagaaaatgt
     1381 agcagtgcca cagcaagaaa ctacggcata ccacacccaa acgctacagc atgccacgtt
     1441 gcaaagtgtg gcagtgccac aacacaaaaa acgtggttta tttggccgtg tgctgaatgc
     1501 cgtctttgat aatgactgag caataagggt gaaagatcat gccgaaactg aaagacattg
     1561 ccctgggaat tattgtagct ccgctgctga tcccgatcat gctgattgca tcgtaccagg
     1621 ataaaaaggc gctgaagaaa gagctggatg aatgccaaaa agaaaaagac caggcatcct
     1681 gattattcaa ttgagctaat caaaagtgtg gaacattgat gaaataatag cgtggaacgt
     1741 gaaaagtcat aaaaaaagcc cgaacaggga tgctcgggct ataaactaga aactacagcg
     1801 ttgaattata aggataatta tacagcattt cgtataaggc gtattatgtt aattttataa
     1861 aaacttaaaa atagatatag aattttttaa ttataagctt ttatcaatta ttaattttat
     1921 aaaggcttct tgtcattcta tatactatag ggtactatac tataataaaa tttaaggaat
     1981 tagctttatg ccaaatcaag tcgaagataa aaaaaagatt ctgacgcgtg tcagacgaat
     2041 caagggtcag gctgaagcga ttgaaaaagc tttagaagac aatgtggaat gtggtgcaat
     2101 tctgcaacaa atatgctctg tgcgtggtgc tattaatggt ttaatgaatg aaatgttgga
     2161 ggttcattta aaggacactt tagtatcagg tgaaaccaca gaacaacaac gaaaagaaga
     2221 attggctgaa atcgccaaga ttctaaaatc atatttaaaa tagatctgta taaggaatta
     2281 ttccatgaag tcacgtgctg ctgttgcgtt tggtcctggt caacctcttc aaattgttga
     2341 ggttgatgtt gcaccaccga agaaaggtga agtattaatc aagatctccc atactggtgt
     2401 gtgtcataca gacgcattta ctttatctgg cgatgatcct gaaggcgttt ttccggctat
     2461 tctcgggcat gaaggtgctg gtgttgtagt tgaggttggt gaaggcgtga ctagcgtcaa
     2521 accaggtgat catgttattc cactttacac tgctgaatgt ggcgagtgcg tattctgtaa
     2581 atctggaaaa tcgaacttgt gtatttccgt tcgcgaaact caaggtagag gcgtaatgcc
     2641 agatggtacc actcgctttt cctacaatgg tcaaccgatt taccactata tgggctgctc
     2701 aacgttcagt gaatacactg tagtggcaga agtatcattg gctaaaatca atcctgaagc
     2761 caatcacgaa catgtctgct tactgggttg tggggtaaca accggtatcg gtgcagtaca
     2821 taacacggct aaagtacaag aaggtgatag tgttgcggtc tttggtttag gtggtattgg
     2881 tttggcagtc gtacaagccg cacgtcaagc taaagcaggg cgtattattg tggttgacat
     2941 gaatccagat aaatttgcgc tggcaaaaga gtttggtgca acggacttct tgaaccctaa
     3001 agactacgat aaaccaatcc agcaagtcat tgtcgaaatg actggctggg gtgtagatca
     3061 ttcatttgaa tgtattggca acgtgaatgt aatgcgtgca gcccttgaaa gtgcacaccg
     3121 tggctgggga caatccgtaa ttattggtgt agctggcgca ggtcaggaaa tctctacccg
     3181 tccattccaa ctggttactg gtcgtaaatg gctaggttct gcctttggtg gcgtaaaagg
     3241 tcgtacgcaa cttccaggaa tggttgaaga tgccatgaaa ggtaaaattc aattagaacc
     3301 atttgtgaca catacgatgg gcttggatca gatcaatgaa gcctttgatc taatgcatga
     3361 aggtaagtca attcgcactg tcattcacta ttgatccacg gatcaatacc taatttttca
     3421 aaaccaacca tcaaggaaac actttaatgg ctactccagt aatttccggt aacgatattt
     3481 tctcccatgt cttcctcggc gcagtcgacc ttaagaagtc ggttgtattc tacgatgccg
     3541 ctctaggtgc cctcggcatt aaaaacctag ggccattcgg taacgattgg gttctgtacg
     3601 gtcgcgacaa acctgctttc atgatcaccc gcccaagcaa tggcgcagaa cccaccagta
     3661 atggtgtgac ggtaggcttc gcagctgcca cccctgcaga ggtaaatgca ttccacgcag
     3721 ccggcctcgc cgctggtggc accgatgaag gtcagcctgg cccacgtggc cacctgccgg
     3781 gtgcttacgc ggcctacctg cgtgatccgg ctggtaataa ggtctgcgcc tacacctttg
     3841 tctaaaacct aggagaagtg aggtcattgt gtaaggactg gttccagcca aaaacaatct
     3901 catcttccac tgcaaccgtg tggcatgccc gctcatgcca cacggtttac ggtactctga
     3961 gcctctatct gctgattaga gctcagaaag gctgttacct cgccttcata gccaaagacg
     4021 gtcaatgagc acgatcggac tgaaagttcg gtgcgtgatg caagaaacac agaatccaaa
     4081 atgagcatcg cggtagtggc tccctgaatt acacacttta ttgtttgtac attgaagtcg
     4141 acatccgacc ttcgtggtgt ggtggcgtct gggtttgtag cagcgatctt catgccagcg
     4201 acaacgctga aaacctgctg agtggtgcgg tgaagcattt ttaactaatc ctggatggaa
     4261 cgaaaattcg tcttgattgg cgcctgagca ccacctcgtt gaaataagga gaaaatccgt
     4321 ggaacgcatt gaacatcacg ccagcttcga gggttggcag gacgtttttc agcatgagtc
     4381 aacagtgctt ggctgcatga tgaggtttgg aatctatctt ccgcctcagg ctgagcatga
     4441 aaagctccca gttctgtact ggctctcagg tctgacctgt accgaacaga acttcatcac
     4501 caaggctgcg gtgcagcggt atgccgcgga gcacggaatc attgtcgtag ccccagatac
     4561 cagcccgcgc ggagaaagcg ttgcagacga tgctgcctat gatctaggtc aaggtgcggg
     4621 tttctatgta gacgccacag agctaccttg gagcgatcac tatcagatgt attcctatgt
     4681 ggtcgacgaa ttaccagcgc tgatcgaagc gaacttccct gcgtcgtcaa gacgcagcat
     4741 cagtgggcac tccatgggcg gtcatggcgc tctggttatt gcgcttcgca accccggtcg
     4801 ctatgcaagt gtctcggcgt tctcgccgat cgtggcgcct acgcaagttc catgggggca
     4861 gaaggctctg gcggcttact tgggcgaaaa gaatctggaa gcctggaagc aatatgacac
     4921 ggtcgagctg attcgcgtgg cgcaagaacg cttaccgctg cttgtcgatc aagggctgag
     4981 cgatgagttt ctcgagcacc agcttcgacc gcaactgttg cgtaccgcgt gtgaacaggt
     5041 agaccacccg ctaacgctca atctgcggcc tggtcatgac catagctact actttatttc
     5101 gacctacttg agcgaacaca tggcccacca tgcggctgca ttgaatcgct gaacattggg
     5161 agaccctgac ttggaaatgt ctgtatagga catttctatt tggttttaag gggctttgtt
     5221 gcataaatac ataaccaact gattttgttc atttaatttt taggttgaaa ttcatacaaa
     5281 cctatttaaa tcagaagctt acaaaatctt tatgcaacaa agcccatatc agataaaaaa
     5341 gtagaggagc taatactaag ccgtgatttc ttggaaaagc tgaaagagct agttaatcga
     5401 acagaaatac catgtagtta actatccatt taacataatg ggcgttatcc gaaatcgtct
     5461 tttataagtt aaataaaatc atgtacttag ctattttaca taacccaaaa ctaccgcaaa
     5521 aatccgcttt ggaaacaaag cggatttttt ttgagcaagt ttgatacttc ttgccaataa
     5581 aattggttaa ataatgatca aaaatagcaa ttccatagag tttttaagcc ttttttaccc
     5641 aaaagaatcc acaatttctg cggataacca ttgggataaa ttttgattat tttttgtacg
     5701 ttttggctta cagcgtttca cgaaattgtc tgtatctatc ccccgatgaa gctcttatga
     5761 ttgatctata tggacaggat gttcaatgac ttaaaacaac aataataatc agtcaggcac
     5821 catgaccatc aggtagtcag ccctacctat gcgctccata ccgaaaaatc ccgacatgac
     5881 gtcgggattt tttattatct aaacttctta aattaaagtt ggatgagcat ctaaaatacg
     5941 aattaaagtc gctgcaggac cagtcggata gcgacggcct tgttcccagt tttgcagggt
     6001 tctcgcgctg atatgcaaac gtgcagcaaa ttcagcctgg gttaagcctg ttttctcacg
     6061 tacttcttta atatccggta attcaagctc tgtgactcga cttgccttta tctccttacg
     6121 cttgatggca ccagcttctt tgattgaagc aaccaggtca tcaaataagt tgttatccat
     6181 gaaattgctc ctttactaat tgacgtagaa tcgaggtttc tttgtctgtc agattatctt
     6241 tcacagactt tggataagcc accaggaaaa agatctgatc atcttctgta acccaataat
     6301 agatcacacg gataccgcca cttttgcctt tattgccctg agcacaacgt actttacgaa
     6361 ttccaccacc attcttgatt aagtcgccct tatcaggctg taccaagaga tcttgctgga
     6421 gctgacgata ctcttcatca ctcacaagct ctttgatttg cttagtaaaa atactggttt
     6481 caatgaataa cataggttta agtacgtcaa tggcgtataa gaattttagc agttttagaa
     6541 taaaaaaacg agagctgtga actctcgttt cgcataagca aaggcttaca aatataaata
     6601 ttaggtataa aaaacccgca tgatagcgag taggtcctga tatttcattc agactataga
     6661 ttttataaaa actatatttc tagatgattt taaatatttt tccacttaag gtactttgaa
     6721 aaagcatttc aaaactaggt cgaatatctt caccatcaac ggtgatatgt tttatattca
     6781 aatgagattg tgtgttggca aactcatgat ccactgtggc ttgaatagcg tgtttaacgc
     6841 caaactggtc ttttacgctg agagtctgtt taaacatata tatttcctta aaaaaattgc
     6901 agtggaaatc ccacataaat tattgatgtt taaaaattta tcgctaagca actttatgat
     6961 ttaatttaaa tagctgattt agagaagtgt aaaatgtacg gccatccaaa tttaaatcaa
     7021 cttcgattcc agattccagt agaactcttt tacctacttc gatttgtttt aaaccttgac
     7081 gtgtattttg cattttatgt agaggaatta atgaaacaat aatttcagta ccatttttag
     7141 attttgcaat taaatcattt acttttaaca acttatatcc tattttttag agattttttc
     7201 aattttgaaa acattttaaa gcttaaaaaa agcacttatg agtgcttttt tattactgct
     7261 tattattact tagatagcaa caatatttac agcattcggt cctttttgac cttgagctac
     7321 actgaattca acttgctggc cttcaaataa ggtcttgaaa cctgaactcg cgatttcgct
     7381 gaaatgagca aaaacgtctg gacctgattc ttgttgaatg aaaccaaaac ctttagtttc
     7441 gttaaaccac ttaacagtac ctttaacaac atttgagttt gacataatat atcctactaa
     7501 tttttaataa ttttttagtc attttattga ctgattaata actttgaaaa taataaagaa
     7561 tgagacttaa aatctaaaaa acgaaggatt atgactaaaa ctgcgatact taaggaagat
     7621 ttaccgaaac aagacttttt ttctagttaa gttaaatata cagtaaaaat aaaaataatc
     7681 aagttttttg atataaatat taatttttct tataaaatcc atattttttt ctttaaaaat
     7741 taattaaatt caataagtta tttattatat tatttaatat tttctttaac ataaaatctc
     7801 ttatgcgaaa ttacatacca actttaacca cattgtggac aggttggagt ttagagataa
     7861 gaaaaatttc tagagcattt gctaaataag ccctatcacg ttcaaagcct atcgtaatga
     7921 tgggcgaatt caaacatgag gtgcgacagt ttcaaaagcc atatgataat caacaagctg
     7981 agcaaatttc tctaatggtg taagccaatc taacgccttt ctaggacgag tattcagtga
     8041 catggcaact tgatttaaat aatgctgatc tgcctgattt aaatcaatcc ctttaggtaa
     8101 atattgccta attaaaccat tcatattttc gcatgtgcct ttttgccagg gtgaatgtgg
     8161 gtcacagaaa tatacatcta tgcctaaatc ttcttcgagt attttatgtt ctgacatctc
     8221 acgtccacgg tcataggtca acgttttacg cagttctgca ggtaaatatt tcagagcttc
     8281 agttaaagcc ttgcgcactg attctgcctt tgcatcaggt aatgttgcca agatacagag
     8341 ccgtgtattt cgttcaataa gtgttgctat cgaactttta ttgtctttac ctttaattaa
     8401 atcagcttcc caatgacccg gtatttttct ttcttgaact tcggctgggc gctcatgaat
     8461 agttttaata tcctgtaata tagaatcttt tttaggttca ccgttagctt ttcgcttttt
     8521 attttcatga cgtagacagg ataataagtc ttttttcaac tcacccttgg gtaatgctcg
     8581 tatcgttgaa taaatcgttg tatggcttac attcattgtt tgatccaaat caggaaatgt
     8641 ctttaaacgc tttgctattt gctgaggaga ccataaacaa cggatcgctt caacaataaa
     8701 tttccagagg attgaatcga ttttgagttt tctgtgacca cgtctacgtc tagcaaaagt
     8761 gttatcagaa gcatattgag cttgataaac gtcattgatg ctatttcttt taagctcacg
     8821 atagatcgta ctaggatgtc ttttaataag ttcagcaaat tttctggctg aaaagccttc
     8881 ttttcttgac tcaagcatta atgcagtacg atcttcaaag ttaagatgat ggtatgacaa
     8941 ttttatatac tccataaacc ctttaaatta attaggtggt ttatgtcgca cttcaagttt
     9001 tactctgcca tggatccagc atcttttaat cttggatcta tcgcaatata ttcagtttta
     9061 tcttcattcc atcggcaata tttcattacc ctggcgctta agccattacc acgataacgg
     9121 ccagcacctc tagaagtgcc aacaccgata taagtcacta aatccccttt gataaacgca
     9181 tatacacctt gtttatcgta attaggaatt ttcccaaaat taaaatgtac tggtgaccat
     9241 tcaggaggct ttcctaatag atctacattc cagtgatgtt caaagaataa ttctgtatta
     9301 cgtattaact cattaagtgt tgaatcttcc actactcatc tctcaaaatt tatagattaa
     9361 atagctttcg gtcacgctca tccgttaaat acttatccat ctgagcaatt agtttagctc
     9421 tggcttgttc atcatcttca gtgattttta acatacagga tccaattaaa atcttgcgtc
     9481 tggtatcgtc ctttcgtgcc tgttctttct ctttggtacg ctctcttgcc agtgctgcct
     9541 gtctttgagc ctttaactgc tttagctttt cttcctgtgc cttgattttg ctgtctagtg
     9601 tttcagtaac gctcattaat ccaataccat cattctggga aaccatccct aacataagcg
     9661 cacttgaata tgcattcaag gtgctatatg tatagtaatg ttttcctcga agagcgcact
     9721 tatgcaaact atgtttgcaa gtccgattgg ggtctcccca ataccccgac aaccgtattc
     9781 acggttgtat ttcactacgt gaaaatttaa aagcgaaaaa tagacatggc gatttaccac
     9841 ttttcagtca aaaccgtagc acgatcagca gggcgttccg ccactgctgc gattgcctat
     9901 cgggcaggtg aaaagatcta ttgtgaacgt gaaggccgag aacatgacta cagccgaaaa
     9961 accggtgtgg aatacaaaga aatttattta ccagaaggcg caccagagca tttaaagaat
    10021 cgagaaaaac tctggaacga agttgaacaa cgggagaccc gaaaaaactc gactgtagcc
    10081 agggaatttg aaattgcctt tccaagtgaa ctgaaccagg aacaacgtct ggccatgctc
    10141 gaagaattat gtgccagcat cgtggaaaga catcaagtag cagtcgatgc ctgtattcat
    10201 gcaccgcata ccggatccgg cagtgatgag cgcaattatc acgcccatat cctaatgagc
    10261 acacgtaaac ttacgccaga aggttttaca gagaaaaccc gggaactcga tcagaaacac
    10321 agtggagaaa tcgatcactg gcgtgaacac tttgccgata tctgcaatgt gcacctgaat
    10381 ctggccggat ccacagcccg ggtcgaccac cgcagctaca aagaccagga gaatggcctg
    10441 gaagccaccc tgcacgaagg accgaaagtc accgaactac gtagaagagg catcgaaacc
    10501 gaaatcagcc gaagcaatga tgaaatcaaa cagagaaatc aggctcagtt gcaatacgac
    10561 aaaaatatgg acgttctaat tgctgaaaat gagataaaac tcagcacact gaaaacagaa
    10621 cagcagatcc aaatcaagaa tagcgctaaa acgccaccaa ttgacgaaaa atccctgttt
    10681 gaggaaaaac agaggggaac ccttggcaaa gtgctaaaac gcgaaatcag cgcaaaagac
    10741 gcgaatctag acctggattt tatgcagcgc aatctcaaac aagccaaata aacctgacca
    10801 agcaccacaa acatcaaaac gagttcaatc agcagcttgc ccaggagatc gtgaaaagcg
    10861 gactcaagca aagtcatgac aaattgcaaa gcctggtcga tcaacacaac gaattaaccc
    10921 agaacaaacc cttactgttt ggaaaaaaag cctgggaagc ccaacgtgat gagatctacc
    10981 aggaacacaa aaagttgaaa ggtcagcatg aacaccagaa aaaacatggt gtgaaggatt
    11041 tattggagaa tgaaaagttc aaagaacatg cctggaagaa atatcaaaaa cagcatccgg
    11101 agaaagccaa gcaataccag accctctacc agtcctacaa gatcgttaaa aaatgtgtgg
    11161 atgagattaa agcagaacaa cagatgaaaa tcagacaaga acaacaactc aaagcacagc
    11221 aacatgcgcc taaaatgaaa tctcgtggca tgagtcgtta aacagagcga gtgtctacga
    11281 gcgaactgag aaaatttgcc catccctttc cctgattaca agctccctat ccatcaatca
    11341 cctgcatacc agagcatccc gcatgggtgc gaactttcca agtttgattc ctaaatcgag
    11401 cgtagcgaga aaaaaagctc atgagcgaag cgaattccga gttgcttttg atcttgcttt
    11461 tgctttttct caaattcacg aaaatatgaa ccaaaaattg ccccgacgaa tcgagcgaaa
    11521 gcgagattca atagagtttg agcgaacgaa gtgaagcgaa aaccaagggc aatttttcac
    11581 tccttgggct tttaattatt ttaaagtttt taaatgcttt tagatatgct gaaagccttt
    11641 gttcacaatg cttttaaggt ttatatgaca ccgtttacct tgcaatatga caccgtttac
    11701 cttgcaatat gacaccgttt accttgcaat atgacaccgt ttaccttgct tatagcacta
    11761 aaattaatta ttgtgtcata gcataataat taatactggt gcttc
//
