LOCUS       NZ_CP096819            14639 bp    DNA     circular CON 05-JUN-2024
DEFINITION  Acinetobacter baumannii strain Mu1984 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP096819
VERSION     NZ_CP096819.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27957725
            Assembly: GCF_030168245.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 14639)
  AUTHORS   Choby,J.E. and Weiss,D.S.
  TITLE     Heteroresistance via beta-lactamase gene amplification threatens the
            beta-lactam pipeline
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14639)
  AUTHORS   Choby,J.E. and Weiss,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2022) Division of Infectious Disease, Emory
            University School of Medicine, 954 Gatewood Road Room 2052, Atlanta,
            GA 30329, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030168245.1-RS_2024_06_05
            Annotation Date                   :: 06/05/2024 04:33:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,885
            CDSs (total)                      :: 3,790
            Genes (coding)                    :: 3,693
            CDSs (with protein)               :: 3,693
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 63 of 97
            Pseudo Genes (internal stop)      :: 10 of 97
            Pseudo Genes (multiple problems)  :: 6 of 97
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096819.1.
            Bacteria and source DNA available from David S Weiss, Emory
            University.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..14639
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="Mu1984"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA:Georga, Atlanta"
                     /lat_lon="33.7911 N 84.3233 W"
                     /collection_date="2012/2015"
                     /collected_by="Georgia Emerging Infections Program"
     gene            1..951
                     /gene="repM"
                     /locus_tag="M1R67_RS19330"
                     /old_locus_tag="M1R67_19320"
     CDS             1..951
                     /gene="repM"
                     /locus_tag="M1R67_RS19330"
                     /old_locus_tag="M1R67_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205341.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLDLFSKMTDAQRHMFANKLSELPEMGRYSQGTESYPQFAV
                     RIAEMLQDSEKIKELAPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            944..1519
                     /locus_tag="M1R67_RS19335"
                     /old_locus_tag="M1R67_19325"
     CDS             944..1519
                     /locus_tag="M1R67_RS19335"
                     /old_locus_tag="M1R67_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096611.1"
                     /translation="MAKLSLSEVSKKFHIDRSTIYRAVRNGRLSRSSDGQFDLSEVIRC
                     FGEPEQISQQIEPSKSDNDESTKKLIIHLENEVKKYQEREERLMQQIDRMQTLIELKTV
                     APATAAPQQDATACDTDMPQHATTQQDNDYKKNNELNIVENVAVPQQETTAYHSQTLQH
                     ATLQNVAVPQPKKRGLFGRVLNAVFNEE"
                     /name="plasmid replication DNA-binding protein"
     gene            1541..1684
                     /locus_tag="M1R67_RS19340"
                     /old_locus_tag="M1R67_19330"
     CDS             1541..1684
                     /locus_tag="M1R67_RS19340"
                     /old_locus_tag="M1R67_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000734097.1"
                     /translation="MKKLKEILQGIAFLFVGIPIMIMASIIDQRAFKKEMDERQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            1990..2265
                     /locus_tag="M1R67_RS19345"
                     /old_locus_tag="M1R67_19335"
     CDS             1990..2265
                     /locus_tag="M1R67_RS19345"
                     /old_locus_tag="M1R67_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="WP_001133626.1"
                     /translation="MPNQVEDKKKILTRVRRIKGQAEAIEKALEDNVECGAILQQICSV
                     RGAINGLMNEMLEVHLKDTLVSGETTEQQRKEELAEIAKILKSYLK"
                     /name="metal/formaldehyde-sensitive transcriptional
                     repressor"
     gene            2287..3396
                     /locus_tag="M1R67_RS19350"
                     /old_locus_tag="M1R67_19340"
     CDS             2287..3396
                     /locus_tag="M1R67_RS19350"
                     /old_locus_tag="M1R67_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995413.1"
                     /GO_function="GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA]; GO:0051903 -
                     S-(hydroxymethyl)glutathione dehydrogenase (NAD(P)+)
                     activity [Evidence IEA]"
                     /GO_process="GO:0006113 - fermentation [Evidence IEA];
                     GO:0009404 - toxin metabolic process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="WP_000842148.1"
                     /translation="MKSRAAVAFGPGQPLQIVEVDVAPPKKGEVLIKISHTGVCHTDAF
                     TLSGDDPEGVFPAILGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECVFCKSGKSN
                     LCISVRETQGRGVMPDGTTRFSYNGQPIYHYMGCSTFSEYTVVAEVSLAKINPEANHEH
                     VCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGGIGLAVVQAARQAKAGRIIVVDMNPD
                     KFALAKEFGATDFLNPKDYDKPIQQVIVEMTGWGVDHSFECIGNVNVMRAALESAHRGW
                     GQSVIIGVAGAGQEISTRPFQLVTGRKWLGSAFGGVKGRTQLPGMVEDAMKGKIQLEPF
                     VTHTMGLDQINEAFDLMHEGKSIRTVIHY"
                     /name="S-(hydroxymethyl)glutathione dehydrogenase/class III
                     alcohol dehydrogenase"
     gene            3449..3847
                     /locus_tag="M1R67_RS19355"
                     /old_locus_tag="M1R67_19345"
     CDS             3449..3847
                     /locus_tag="M1R67_RS19355"
                     /old_locus_tag="M1R67_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="WP_000235176.1"
                     /translation="MATPVISGNDIFSHVFLGAVDLKKSVVFYDAALGALGIKNLGPFG
                     NDWVLYGRDKPAFMITRPSNGAEPTSNGVTVGFAAATPAEVNAFHAAGLAAGGTDEGQP
                     GPRGHLPGAYAAYLRDPAGNKVCAYTFV"
                     /name="VOC family protein"
     gene            4321..5154
                     /gene="fghA"
                     /locus_tag="M1R67_RS19360"
                     /old_locus_tag="M1R67_19350"
     CDS             4321..5154
                     /gene="fghA"
                     /locus_tag="M1R67_RS19360"
                     /old_locus_tag="M1R67_19350"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439570.1"
                     /GO_function="GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="WP_000439570.1"
                     /translation="MERIEHHASFEGWQDVFQHESTVLGCMMRFGIYLPPQAEHEKLPV
                     LYWLSGLTCTEQNFITKAAVQRYAAEHGIIVVAPDTSPRGESVADDAAYDLGQGAGFYV
                     DATELPWSDHYQMYSYVVDELPALIEANFPASSRRSISGHSMGGHGALVIALRNPGRYA
                     SVSAFSPIVAPTQVPWGQKALAAYLGEKNLEAWKQYDTVELIRVAQERLPLLVDQGLSD
                     EFLEHQLRPQLLRTACEQVDHPLTLNLRPGHDHSYYFISTYLSEHMAHHAAALNR"
                     /name="fghA"
     gene            5665..5952
                     /locus_tag="M1R67_RS19365"
                     /old_locus_tag="M1R67_19355"
     CDS             5665..5952
                     /locus_tag="M1R67_RS19365"
                     /old_locus_tag="M1R67_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            5939..6253
                     /locus_tag="M1R67_RS19370"
                     /old_locus_tag="M1R67_19360"
     CDS             5939..6253
                     /locus_tag="M1R67_RS19370"
                     /old_locus_tag="M1R67_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            6381..8793
                     /gene="znuD2"
                     /locus_tag="M1R67_RS19375"
                     /old_locus_tag="M1R67_19365"
                     /pseudo=""
     CDS             6381..8793
                     /gene="znuD2"
                     /locus_tag="M1R67_RS19375"
                     /old_locus_tag="M1R67_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /name="znuD2"
     gene            9098..9559
                     /locus_tag="M1R67_RS19380"
                     /old_locus_tag="M1R67_19370"
     CDS             9098..9559
                     /locus_tag="M1R67_RS19380"
                     /old_locus_tag="M1R67_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(9880..10413)
                     /locus_tag="M1R67_RS19385"
                     /old_locus_tag="M1R67_19375"
     CDS             complement(9880..10413)
                     /locus_tag="M1R67_RS19385"
                     /old_locus_tag="M1R67_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005207483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000965055.1"
                     /translation="MLYEAYYPSEGSDNHPIKIFYGFVQEGFEKGHQLTLVVFYPELTG
                     TSLFNLMDETKAGLANFLLQKHVYGVGASHIKVAFVCEHVNKRDFYTEIWGVDVFENYA
                     LEQLEQQPKSLLSGILGNNSKEVSIHSSYLNIGLTDSIKLIDEGHLDTEQTAYLLNKLK
                     EKTDYGNFNKIFGE"
                     /name="hypothetical protein"
     gene            complement(10481..12298)
                     /locus_tag="M1R67_RS19390"
                     /old_locus_tag="M1R67_19380"
     CDS             complement(10481..12298)
                     /locus_tag="M1R67_RS19390"
                     /old_locus_tag="M1R67_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="WP_000903026.1"
                     /translation="MLHNFFMSYYGYLINGLHLKPLFVGFANTLFALAIIVIFTMIVVG
                     IKKIYKAHDPVYEIGYNIVLLAVVVLAHTYLLSYITNLLPNNYVVVPEKNYFFSWAVII
                     GLYVYLYFTGKKSKPRVERIKKESAEGYGTLLSELKRGFENKTPLIAFTDQTLPKKQIK
                     EMEQKYLDWSFHRESLRVTNEDRGIVLGAPGSGKTTFLISQIVEWMKSGRSFVATDVKP
                     EIWAILKANNVFKIFGYTDWVFNPTDVDSDHYNIFSEVKDSAELNEILNIIIKDDTADS
                     VVFNDLARRILKAVLIELGDKASLVNAQNYINSMDSNDELLKALRQSEKSSVSNIAKEV
                     SRTAQNDRLLASVMTAVSKAFEFLDDDRIRVTTSDNTQGFYLKEVLMKPKQAVFLQFDQ
                     QYKSSTATLFGAMVAHTMRILQANQNREAVFIALDEIINCAPIPKFTDLLNTIRSANMP
                     TFLYLQSLEGLNRLYGANSDKMFMGSSNLKIVFRIGDIESAEECSRLVGQTETTYISET
                     AGTSQTSGTSSSSRASSSSSNRSQNTGTTKSIKLESIIEPAEFIKLPICTAVVMYNGSY
                     GTLEMPKYYECYNMPKRTNLKTIRDFKVA"
                     /name="type IV secretion system DNA-binding
                     domain-containing protein"
     gene            complement(12299..12538)
                     /locus_tag="M1R67_RS19395"
                     /old_locus_tag="M1R67_19385"
     CDS             complement(12299..12538)
                     /locus_tag="M1R67_RS19395"
                     /old_locus_tag="M1R67_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000510443.1"
                     /translation="MGIASEIRSSLPLCGRCINCKLLIWNDKAELKSTQNLIKFRSSEA
                     SFYYTVRCSWLKSPVSEPQFLDTCEGKQQQKGSD"
                     /name="hypothetical protein"
     gene            complement(12539..13432)
                     /locus_tag="M1R67_RS19400"
                     /old_locus_tag="M1R67_19390"
     CDS             complement(12539..13432)
                     /locus_tag="M1R67_RS19400"
                     /old_locus_tag="M1R67_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005207487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal domain-containing
                     protein"
                     /protein_id="WP_000171721.1"
                     /translation="MTLEYQFDQLCNKFREGSHATQANRRAMLKLFTEQLAKNGYDVKT
                     MSPKDLKGRHVNKLLEQWRKDGVSTGTIKNRMVAVRWWAEKVGNSGAVKDNATYKIENR
                     VYVTNENKSISLKDLDLSKTDPNIAQSLRLQDAFGLRREESMKFQPEYALDGQKIENAK
                     YIRLKDSWTKGGRPRTIPITNEKQRQELKNAYAQAVKNGGSMIPKEKSYKSHKANFEAV
                     THALGVGQTHGLRHGYAQTRYRELMGFDCPAVGGSRSLSADEQVKDKEIRMLISEELGH
                     SRINITSVYLGSWSKN"
                     /name="phage integrase N-terminal domain-containing
                     protein"
     Typing          2..951
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.266"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagagatt tagttgtaaa agacaatgcc ttaatcaacg caagctataa cttagactta
       61 gtagaacaac gtttaatttt attggctatt gttgaagcaa gggaaagtgg gaaagggatt
      121 aatgcaaatg atccattaga agttcatgca gatagttata tcaatcaatt tggtgtacac
      181 cgtaatacag cttatcaagc cttaaaagat gcttgtaagg atttattcgc gcgtcaattt
      241 agctatcaag agaaaaaagc taatgggaat atccgaaatg ttatgagtcg ttgggtatct
      301 caaattgctt ataacgacaa tgaagcaact gtagacttaa tatttgcacc tgctgttgtt
      361 cccttcataa ccagactgga agaacaattt actaaatatg aattacagca agttagtagt
      421 cttagtagcg cttatgccat tcgcttatat gagcttctaa ttcagtggcg aagcactggt
      481 aaaaccccaa ctatagaact acaagaattt agaaagaagt taggcgttct tgataatgaa
      541 tatttacgga tggctcattt aaaagagcgt gttttagagc tttcaattaa acaaataaat
      601 gagcatacgg atataactgt aaaatatgaa cagcataaaa gaggacgttc tatttcagga
      661 ttttctttta cctttaaaca gaaaaagaag gatagcccat caatagaaag agatccgaat
      721 accttagacc ttttttcaaa gatgaccgat gctcaacggc atatgtttgc aaacaaactt
      781 tcagaactcc ctgaaatggg tcgctattca caaggaactg aaagctatcc tcaatttgct
      841 gttcgtattg ctgagatgct acaagactct gaaaaaatca aagaactagc cccataccta
      901 aaaaaagtgg gatacatgcc atcaaataaa aaggacacag taaatggcta agttatcgct
      961 aagtgaagta tctaaaaaat ttcatattga taggtcaacc atttatagag ctgtacgtaa
     1021 tgggcgttta tcacgctcta gtgatggtca atttgacctt tcggaagtga ttagatgctt
     1081 tggggagcct gagcaaatat ctcaacaaat tgaaccatct aaatcagata atgatgaatc
     1141 tacaaaaaaa ctcattattc atttagaaaa tgaagtcaaa aaataccaag aacgtgaaga
     1201 gcgtttaatg cagcaaatcg accgtatgca gacgcttatt gagttaaaaa ctgttgcacc
     1261 cgccacggca gcgccacaac aagatgctac ggcatgcgac accgatatgc cacagcatgc
     1321 gacaacacaa caagacaatg attataaaaa gaataatgaa ttaaatattg tagaaaatgt
     1381 ggctgtgcca cagcaagaaa ctacagcata ccacagccaa acgctacagc atgccacatt
     1441 acaaaatgtg gcagtgccac aacctaaaaa acgaggctta tttggccgag tactgaatgc
     1501 ggtattcaat gaagagtaag ctctccaagg agcattgttt atgaaaaaac tgaaagagat
     1561 cctgcaaggt attgcctttt tatttgttgg gattccgatc atgatcatgg ccagcatcat
     1621 agatcaacgt gccttcaaaa aagagatgga tgaacgccaa aaagaaaaag accaggcatc
     1681 ctgattattc aattgagcta atcaaaagtg tggaacattg atgaaataat agcgtggaac
     1741 gtgaaaagtc ataaaaaaag cccgaacagg gatgctcggg ctataaacta gaaactacag
     1801 cgttgaatta taaggataat tatacagcat ttcgtataag gcgtattatg ttaattttat
     1861 aaaaacttaa aaatagatat agaatttttt aattataagc ttttatcaat tattaatttt
     1921 ataaaggctt cttgtcattc tatatactat agggtactat actataataa aatttaagga
     1981 attagcttta tgccaaatca agtcgaagat aaaaaaaaga ttctgacgcg tgtcagacga
     2041 atcaagggtc aggctgaagc gattgaaaaa gctttagaag acaatgtgga atgtggtgca
     2101 attctgcaac aaatatgctc tgtgcgtggt gctattaatg gtttaatgaa tgaaatgttg
     2161 gaggttcatt taaaggacac tttagtatca ggtgaaacca cagaacaaca acgaaaagaa
     2221 gaattggctg aaatcgccaa gattctaaaa tcatatttaa aatagatctg tataaggaat
     2281 tattccatga agtcacgtgc tgctgttgcg tttggtcctg gtcaacctct tcaaattgtt
     2341 gaggttgatg ttgcaccacc gaagaaaggt gaagtattaa tcaagatctc ccatactggt
     2401 gtgtgtcata cagacgcatt tactttatct ggcgatgatc ctgaaggcgt ttttccggct
     2461 attctcgggc atgaaggtgc tggtgttgta gttgaggttg gtgaaggcgt gactagcgtc
     2521 aaaccaggtg atcatgttat tccactttac actgctgaat gtggcgagtg cgtattctgt
     2581 aaatctggaa aatcgaactt gtgtatttcc gttcgcgaaa ctcaaggtag aggcgtaatg
     2641 ccagatggta ccactcgctt ttcctacaat ggtcaaccga tttaccacta tatgggctgc
     2701 tcaacgttca gtgaatacac tgtagtggca gaagtatcat tggctaaaat caatcctgaa
     2761 gccaatcacg aacatgtctg cttactgggt tgtggggtaa caaccggtat cggtgcagta
     2821 cataacacgg ctaaagtaca agaaggtgat agtgttgcgg tctttggttt aggtggtatt
     2881 ggtttggcag tcgtacaagc cgcacgtcaa gctaaagcag ggcgtattat tgtggttgac
     2941 atgaatccag ataaatttgc gctggcaaaa gagtttggtg caacggactt cttgaaccct
     3001 aaagactacg ataaaccaat ccagcaagtc attgtcgaaa tgactggctg gggtgtagat
     3061 cattcatttg aatgtattgg caacgtgaat gtaatgcgtg cagcccttga aagtgcacac
     3121 cgtggctggg gacaatccgt aattattggt gtagctggcg caggtcagga aatctctacc
     3181 cgtccattcc aactggttac tggtcgtaaa tggctaggtt ctgcctttgg tggcgtaaaa
     3241 ggtcgtacgc aacttccagg aatggttgaa gatgccatga aaggtaaaat tcaattagaa
     3301 ccatttgtga cacatacgat gggcttggat cagatcaatg aagcctttga tctaatgcat
     3361 gaaggtaagt caattcgcac tgtcattcac tattgatcca cggatcaata cctaattttt
     3421 caaaaccaac catcaaggaa acactttaat ggctactcca gtaatttccg gtaacgatat
     3481 tttctcccat gtcttcctcg gcgcagtcga ccttaagaag tcggttgtat tctacgatgc
     3541 cgctctaggt gccctcggca ttaaaaacct agggccattc ggtaacgatt gggttctgta
     3601 cggtcgcgac aaacctgctt tcatgatcac ccgcccaagc aatggcgcag aacccaccag
     3661 taatggtgtg acggtaggct tcgcagctgc cacccctgca gaggtaaatg cattccacgc
     3721 agccggcctc gccgctggtg gcaccgatga aggtcagcct ggcccacgtg gccacctgcc
     3781 gggtgcttac gcggcctacc tgcgtgatcc ggctggtaat aaggtctgcg cctacacctt
     3841 tgtctaaaac ctaggagaag tgaggtcatt gtgtaaggac tggttccagc caaaaacaat
     3901 ctcatcttcc actgcaaccg tgtggcatgc ccgctcatgc cacacggttt acggtactct
     3961 gagcctctat ctgctgatta gagctcagaa aggctgttac ctcgccttca tagccaaaga
     4021 cggtcaatga gcacgatcgg actgaaagtt cggtgcgtga tgcaagaaac acagaatcca
     4081 aaatgagcat cgcggtagtg gctccctgaa ttacacactt tattgtttgt acattgaagt
     4141 cgacatccga ccttcgtggt gtggtggcgt ctgggtttgt agcagcgatc ttcatgccag
     4201 cgacaacgct gaaaacctgc tgagtggtgc ggtgaagcat ttttaactaa tcctggatgg
     4261 aacgaaaatt cgtcttgatt ggcgcctgag caccacctcg ttgaaataag gagaaaatcc
     4321 gtggaacgca ttgaacatca cgccagcttc gagggttggc aggacgtttt tcagcatgag
     4381 tcaacagtgc ttggctgcat gatgaggttt ggaatctatc ttccgcctca ggctgagcat
     4441 gaaaagctcc cagttctgta ctggctctca ggtctgacct gtaccgaaca gaacttcatc
     4501 accaaggctg cggtgcagcg gtatgccgcg gagcacggaa tcattgtcgt agccccagat
     4561 accagcccgc gcggagaaag cgttgcagac gatgctgcct atgatctagg tcaaggtgcg
     4621 ggtttctatg tagacgccac agagctacct tggagcgatc actatcagat gtattcctat
     4681 gtggtcgacg aattaccagc gctgatcgaa gcgaacttcc ctgcgtcgtc aagacgcagc
     4741 atcagtgggc actccatggg cggtcatggc gctctggtta ttgcgcttcg caaccccggt
     4801 cgctatgcaa gtgtctcggc gttctcgccg atcgtggcgc ctacgcaagt tccatggggg
     4861 cagaaggctc tggcggctta cttgggcgaa aagaatctgg aagcctggaa gcaatatgac
     4921 acggtcgagc tgattcgcgt ggcgcaagaa cgcttaccgc tgcttgtcga tcaagggctg
     4981 agcgatgagt ttctcgagca ccagcttcga ccgcaactgt tgcgtaccgc gtgtgaacag
     5041 gtagaccacc cgctaacgct caatctgcgg cctggtcatg accatagcta ctactttatt
     5101 tcgacctact tgagcgaaca catggcccac catgcggctg cattgaatcg ctgaacattg
     5161 ggagaccctg acttggaaat gtctgtatag gacatttcta tttggtttta aggggctttg
     5221 ttgcataaat acataaccaa ctgattttgt tcatttaatt tttaggttga aattcataca
     5281 aacctattta aatcagaagc ttacaaaatc tttatgcaac aaagcccata tcagataaaa
     5341 aagtagagga gctaatacta agccgtgatt tcttggaaaa gctgaaagag ctagttaatc
     5401 gaacagaaat accatgtagt taactatcca tttaacataa tggcgattat acgaagtaca
     5461 cttgtatatt aatataaata tcaactactt aataaaaata gaaaaatccc aaaatgccca
     5521 aaaagccgac tttgaaacaa gtcggctttt ttatgagcag ttttgatact tcttgccaat
     5581 aattttgact aaaaattaca cgaatctggt tgagtgatac aattttgtga tacattattg
     5641 tatatacaaa tttgaatgtt ttttatggaa cagtattttg aatgggatga ggctaagaat
     5701 cgaaagaatc agaaaaagca tgatatctct ttcgaaactg caagccttgt ttttgaagat
     5761 ccattacgga tctcaatcca agacagacat accaatggtg aagaacgttg gcaaaccatt
     5821 ggacgagtta aaggcgtact aatgttattg gtggctcaca ccatttttga tgaagatgac
     5881 tgtgaaatca tccgaatcat tagtgcaagg caagtcacta aagcggagcg aaacaaatat
     5941 gagcatggtt agatactcac acaaagagct gaatgagaaa tttggtgaga agcaagatgc
     6001 tgaaattcaa cgcttgcttg ctaaaggcac ggttcctgat gatcagctag atctatcaga
     6061 tattcctgaa attacagact ggagcaatgc tgtacgccaa aatcaatttt accgcccagt
     6121 aaagcaacaa acttcaattc ggttagatgc tgatgtactt gcttggttca aagcacaagg
     6181 caaaggttat caaacacgta tgaatgaaat tttacgagat gctatgctaa aagaattaaa
     6241 aaatcatcaa taagaatggg agcttaggct cccatttttg acttcatttt taggggggat
     6301 taccattcaa aaatttaaat agtagatttt ataaattttt aagtgttata ttataatata
     6361 acattaaatt tggttgtttt atgctaaata aaagtaaact atttttagct ttaattactt
     6421 taggggcctc gaaaatttta ttggcagctg aagggcctgt tactacctta aatacaatag
     6481 tattaactgc acaatctgat gagttagggt ctgagctgtt aggcaaaagt ctaaatgtta
     6541 gtaatcagtt tattgatact agtaaactta aacagcgttc gacaactcta ggggatgcct
     6601 tgggtacgga attaggaatt cattctaatc aatatggagg tggagcatct actcctatta
     6661 ttcgtgggca ggaaggaaaa cgtattaaag ttttacagaa taatgctgac gtgttggata
     6721 tgtctaatat gtccccagat cacgctgtca cagttgaacc aagtttagct aagtctattg
     6781 aaattatacg tggtgcaagt acccttttgt atagttcaaa tagtgcggca ggggtagtca
     6841 atgtcattga ctataaaatt ccaacccaaa tgcctcaaga cggattagag ggtaatacta
     6901 cattacgttt taatactggc agtaatgaga agttgaccac agcaggtgta acagttggct
     6961 taagcccaca tgtagcttta cgggcagaag ggttatatcg taatgctggt aactataaaa
     7021 ctcctcatta ccagtctagc tcttataatt ctttagaaga tcttgaaaac caaaacagta
     7081 tttataaaaa tttaaaatat ttaccagaaa gctgggcaga gtcgagagta ggtacgcttg
     7141 gattatcctg gatagacgat aatacttatt taggagtttc ttatacacat cgtcatgatg
     7201 aatatggttt accagcccat agccatttat atgaaggttg tggtgcctct gcaatcggta
     7261 tcgattcacg aattagtgga ttaaaaaatt atcttttata ttatcctcaa ttgatggatg
     7321 agcaagatat taattacata aatccacgtc ctgattgtca tcagcataat catattcatg
     7381 aaactaattt ctctcataat gctccttata ttgatttaaa tactcgacgt tatgatgtgc
     7441 gtggagaatt cactcaacca tttaccggta ttgacaaaat tcgtacctca ttgagttata
     7501 tagattattt tcacaatgaa ctcgagggag ataagattac taactttttt aaaaatacag
     7561 gaaaagtagg ccgcatagag ttatcacatc agccattagg ggaattaacc ggtatcttgg
     7621 ggctacaata tttggaacag gataatagtg ctttatcacc agtccattct caagaatggc
     7681 cataccacct atctagataa tcagcagcta ttaaatcgaa atgtcactaa aaactttagt
     7741 gtatttggcc tagaaaaata taactggaac gactttactt tcgaactagg tgctcgtata
     7801 gaaaagcaga aagttagtat ggattatgat atagaaaaaa ttaaagattc aatgaaacct
     7861 tggcctaata aatataacag tccttatgta gaaaaaaata ataaaatcag ggctcaaaat
     7921 ttaaaatcta ttttagaagc tgttcaacct aacaaagaaa cagctttttc ttatgcaggt
     7981 acagtccact ggagatttgc tccaaattat atcttatcat taacaggtac acatcaagaa
     8041 agattaccta atgcacaaga aatgtatact catggtatgc atctcgctac taattctttt
     8101 gaaataggta accgctttct aagaaaagag aaatccaata atttagaaat cagtctggcc
     8161 tataaggatg atttacttga ttatcaaatc agtacttact attatgattt tgataactat
     8221 atatatttac agacactcaa tgaagtttta ggaactacaa aagttagaga tcagcataca
     8281 ctacgtatta atcattatag ccagtctgct gctaattttt atggattaga gggcaacata
     8341 gggtatcaat ttaattctgt ttatcacggc tctctttttg gagattatgt gaaaggccgt
     8401 ttaacaaatt tacctgatgc tgtaattgca tacgatatct ggaatagaga accgacttta
     8461 gctccccaaa aggatcgtta cactcctaga ttacctcctg ctcgtttggg tacacgacta
     8521 aaagctgatt ttgatgaatc gcttaaaggt gaaatagaat attatcgagt ctttaaacaa
     8581 gataatattt ctaaatttga gcaggttaca tcaggttata atatgcttaa tatgacttta
     8641 gcatataaaa ataagctatc tcacacagaa tatgatttat tctttaaagc gaataattta
     8701 cttgatcaga aagtctatgc ccatgaaaca tttttgccat atattcccca aattggaaga
     8761 aattttagtt taggcttaaa tcttaatttc tagataagat actgtaatct tctaagaaaa
     8821 tttcttagaa gatttttctt ataaaatgat tagattaata attaaggtaa gcgtccgctg
     8881 aatcccatac ctatttttta attcgattta ggtttccagc agtgtggcag taactcttca
     8941 atctgggtca ctttatgtgt cggcagcctt ttcagcactg aatagccacc gattttgtag
     9001 acacctttta gctagataaa aagcaatttt tttgatcttt aaagaatgag gatataagct
     9061 gtttaaaact taattctaat tggttaatag gtgaaaaatg aaattagcag aagctctctt
     9121 acttcgaagt gatcaacaaa aaaaaattat ttcactaaaa caacgtatca atgcaaatgt
     9181 attggtacaa gatggtgatc aaccatctga agatccaaat gaactactaa aacaagtatt
     9241 ttctttaatt caagaatttc aaaaattaag ttatgcaatt catgaaacta atgctttaac
     9301 aaaattaaat gatggccgtt ctctacttgc tttattgact ttgcgtgatg aatttgtaga
     9361 acagcacaaa accctaactg cagcaataag taatacatct cgtgaatctg atcgttatag
     9421 tacgcgggaa ataaagtggc ataaagttat tccagtttca tcattacaaa aacaagctga
     9481 tgatattagt cttaaattac gtgatttaaa cgtgttaatt cagtcgacta attggaaaat
     9541 tgatttgatt gaagcctaat agtcctttag atataatcaa gttgaacata ctgggcgagt
     9601 acaagatccc ttacttccac aacataggat tgtaaataaa gtgtagtggc ttgcaaagca
     9661 agtggataag cttaaaattt taagtacaaa tcaatccatc acttggcata actcaaatct
     9721 ttgtgcaaac aaagttacac gttacttata actaccatgt actgacagta tgttcacctc
     9781 aattactaaa aataaagaaa tgaacaagca tcagctcaac tacatttcta tttttaattc
     9841 agttcggcat ttaacataaa atctcttata cgaaatcttt tactcaccaa aaattttatt
     9901 aaaatttccg taatcagttt tttctttcag tttatttaaa agataagccg tttgctccgt
     9961 atctaaatgc ccttcatcaa tcaatttgat tgaatcagtt aatccgatgt tcaaataact
    10021 tgaatgaata ctgacttcct tagagttatt tcccaaaatg ccgctgagta aacttttagg
    10081 ttgttgttcg agttgctcta aagcgtagtt ttcaaaaacg tctacgcccc agatttcagt
    10141 atagaagtct cttttattga catgctcaca tacaaaagcc actttaatgt gtgaagctcc
    10201 aacaccataa acatgtttct gtagtaaaaa atttgctaga ccagccttcg tctcatccat
    10261 caaattaaac aaagatgtac ctgttaattc aggataaaaa accaccaaag ttaattgatg
    10321 tcccttttca aacccctctt gcacgaagcc ataaaagatt tttattggat gattgtcgct
    10381 accctcactt gggtaatagg cttcatataa cactttttct agtactccta tattttcgtg
    10441 aaccgtacta aggtttttcg ttattaaaaa gttttgagtt ttaagcgact ttaaagtccc
    10501 taattgtttt taaattagtg cgtttgggca tgttgtagca ctcatagtat ttcggcattt
    10561 ccaatgttcc gtaagagccg ttatacatca caacagccgt acagatcggc aacttaataa
    10621 attcggcagg ctcaataata gattccaatt taatggactt ggttgtacct gtattttgcg
    10681 acctattgga agatgaactg cttgcccgac tcgatgaact tgtaccgctc gtctggcttg
    10741 tacctgccgt ttcagaaata tatgtcgttt cagtttgacc gactaatcga ctgcattcct
    10801 cagccgactc aatgtcccca attctaaaaa caatttttaa attgctagac cccatgaaca
    10861 tcttgtcgct atttgctcca tacaagcgat ttaagccctc taaactttgc agatagagga
    10921 atgtaggcat atttgcagaa cgtatcgtat tgagcaaatc agtgaatttt gggatagggg
    10981 cacaattaat aatttcatct aaggcaatga ataccgcctc acgattttga tttgcctgca
    11041 aaatacgcat ggtatgcgcc accatcgccc caaataatgt agctgttgaa ctcttatact
    11101 gttggtcaaa ctgcagaaat acggcttgtt ttggcttcat taatacttct tttaaataga
    11161 acccttgggt attgtctgat gtagttactc gaatacgatc atcatcaaga aactcaaatg
    11221 cttttgaaac agccgtcatc acagatgcaa gcaagcgatc attttgggct gttcgagaaa
    11281 cttctttagc aatatttgaa acactggatt tttcagactg tcttaatgcc tttaacagct
    11341 catcattact atccatactg ttaatataat tttgagcatt caccagtgat gccttatcac
    11401 caagttcgat gagaaccgcc ttaagaatac gtctagcaag gtcattaaaa accactgaat
    11461 cggctgtatc atctttgatg ataatgttta aaatctcatt gagttctgct gaatccttta
    11521 cttctgaaaa aatattgtag tggtcactat ccacatccgt tggattaaaa acccaatcgg
    11581 tataaccgaa aattttaaag acattgtttg ctttgagaat cgcccaaatt tctggcttta
    11641 cgtctgttgc aacgaagcta cgacccgact tcatccactc cacgatttga gagattaaaa
    11701 acgttgtttt acctgaacca ggtgcgccta acacaatgcc ccgatcttca tttgtcaccc
    11761 ttagactttc acgatggaac gaccaatcaa gatatttttg ttccatctct ttaatttgct
    11821 ttttgggtaa agtctgatcc gtaaaggcaa ttaatggcgt tttattttca aatccacgct
    11881 ttaattcgct taacaatgtc ccatagcctt cggcagactc tttttttatt cgttcaacac
    11941 ggggttttga cttcttccca gtaaaataca gataaacata taacccaatg attacagccc
    12001 atgaaaagaa gtaatttttt tctggtacaa caacataatt atttggaagt aagttggtga
    12061 tataactcaa tagataggta tgcgctaaaa cgactacagc aagcagaaca atgttatagc
    12121 cgatctcata tactggatca tgtgctttat aaattttctt tataccgaca acaatcatcg
    12181 taaaaattac aataattgct aaagcaaaca gcgtatttgc aaaaccaaca aacagcggtt
    12241 taaggtgcaa tccatttatt aaatagccgt aatagctcat aaaaaaatta tgtagcattt
    12301 agtcactccc tttctgctgc tgtttacctt cacaggtatc tagaaattga ggttcagata
    12361 caggactttt gagccatgaa caacgaacgg tataataaaa agatgcttct gagcttctaa
    12421 atttaattaa attttgagta gattttagtt ccgctttatc attccaaatc aatagcttgc
    12481 aattaataca acgaccacac aacggcaaac ttgatcttat ttctgatgca atacccatct
    12541 aatttttact ccatgaaccc aagtacacgg acgtaatatt gattcgacta tgtcccaatt
    12601 cttcactaat taacatacgg atttctttat cttttacttg ctcgtcagcc gacaatgaac
    12661 gagaaccgcc tacagcaggg caatcgaaac ccattaattc acggtatctg gtctgagcgt
    12721 agccatgtcg taaaccatgc gtttgtccca cacctagcgc atgcgtgact gcttcaaaat
    12781 tggctttgtg ggacttatag cttttttctt tcggaatcat tgaaccgcca tttttcacag
    12841 cttgagcgta ggcatttttg agttcttgac gttgtttttc gttggttatt ggaatagttc
    12901 gaggtcttcc gcctttcgtc caactgtctt ttaaacggat atatttagca ttttcaattt
    12961 tctgaccatc taaagcatat tcaggttgga acttcatgct ttcttcacgt ctaagcccga
    13021 aagcatcttg taaacgtaag ctttgcgcta tgtttggatc agttttagac aaatctaaat
    13081 cttttaaaga aatagattta ttttcattgg tcacatagac acgattctct attttataag
    13141 ttgcattgtc tttgacagca ccactattac caactttttc agcccaccac cttacagcca
    13201 ccatccgatt ttttattgtt ccagtggaca caccatcttt acgccattgc tcaagcaatt
    13261 tatttacatg ccgacctttt aaatctttag gcgacatggt tttaacatca taaccattct
    13321 tggcaagctg ttcagtaaac agtttcagca tagctcgcct atttgcttgt gtggcgtgtg
    13381 aaccttcacg gaacttattg cagagctgat caaattgata ctctaaggtc atgtgtcctc
    13441 ttttattcga gatgctttat tgatgagtac agcacagctt tatgctgggc tacggaatca
    13501 atgaagcatc atttttttaa tttataacta tttgagatcc gatgtactgg ttagtgttaa
    13561 gagccgaatt aacgttttat caaagtcaat tatagaaatt ggttcgataa aaaatgctca
    13621 aaagtgggta taaaaattcg agaatcttta aatccaaata tatgaatact tataaaaata
    13681 aaccgtttct gcatatagga tgagagcgat taaaagtatt tgatctccaa tattaagttt
    13741 gtgtttaagc tgaaaaacat aatgaaagtc agcaaagaaa agttatgaaa aaacaagaaa
    13801 gtttgatcat tatatcgttg atttagttac tgtcacgcat ctataactga atatttcgtg
    13861 ttcaattgta actgttataa tctttttccc agtcgtcact actgataagt agtgacgact
    13921 ggggagcatg gccactgttt acgcatatca aaaagggcgg tcgccacccg ttcgggatgg
    13981 ctctgctact ccctgaaaaa ttcagatggg agctggtagt cagagggaat aagttttgag
    14041 atttagattt tttctggtcg ctcacactcc gttcgctttg tacttttctt tttgacttaa
    14101 acaggtttaa accgagcgag tgtctacgag cgattgagca ttataaaaat ttcgcccctg
    14161 ccttttccct gattacaagc tccctttcga ttttaaacgg tagatacaga gcatcccgaa
    14221 tgggtgcgaa caaacttaat ttgaatctag accgagcgaa gcgagtaaaa aagcttatga
    14281 gcgaagcgaa ttccgagttg cttttgcttt ttcttaaagt cacgaaaata ttaaccaaaa
    14341 aattacccct cgaatcgagc gaaagcgaga ttcaatagag tttgagcgaa gcgaaaacca
    14401 agggtaattt ttcattccct gtgcttttaa ttattttaaa ttttttaaat gcttttagat
    14461 ggcttgaaag ccttatgaat aaagggtttc atctcttata tgtccacgtt taccttgcaa
    14521 tatgtccacg tttaccttgc aatatgtcca cgtttacctt gcaatatgtc cacgtttacc
    14581 ttgcattagt acacaaataa tattaacgtg tatttataca caataaaaaa atagtggct
//
