LOCUS       NZ_CP096761            11194 bp    DNA     circular CON 21-MAY-2024
DEFINITION  Acinetobacter baumannii strain 5653 plasmid p5653_2, complete
            sequence.
ACCESSION   NZ_CP096761
VERSION     NZ_CP096761.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN24114648
            Assembly: GCF_023362195.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Gao,Y., Li,H. and Wang,H.
  TITLE     Origin, phylogeny, and transmission of the epidemic clone ST208 of
            carbapenem-resistant Acinetobacter baumannii on a global scale
  JOURNAL   Microbiol Spectr (2022) In press
REFERENCE   2  (bases 1 to 11194)
  AUTHORS   Gao,Y., Li,H. and Wang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2021) Department of Clinical Laboratory, Peking
            University People's Hospital, No.11 Xizhimen South Street, Beijing
            100044, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 340x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023362195.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 04:16:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,051
            CDSs (total)                      :: 3,957
            Genes (coding)                    :: 3,873
            CDSs (with protein)               :: 3,873
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 28 of 84
            Pseudo Genes (incomplete)         :: 49 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 10 of 84
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096761.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11194
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="5653"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="p5653_2"
                     /geo_loc_name="China:Liaoning"
                     /collection_date="2013-05-09"
                     /collected_by="Yue Gao"
     gene            295..1230
                     /gene="repM"
                     /locus_tag="M1S26_RS20240"
                     /old_locus_tag="M1S26_20240"
     CDS             295..1230
                     /gene="repM"
                     /locus_tag="M1S26_RS20240"
                     /old_locus_tag="M1S26_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001292329.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLREASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YESQSGIIFLRFTQDIVPLITRLEENFTKYELQQVSRLSSSYAIRLYELLIQWRSAGKT
                     PLFDLSIFRQQLGVKPHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFTFKQKKMSNLPIKNKRDPDTIDIFSKMTDAQRHLFSHKLSELPEMGKYSHGTESYPQ
                     FAVRIAEMLQNPEKFKELYPYLQKVGFKAA"
                     /name="repM"
     gene            1308..1826
                     /locus_tag="M1S26_RS20245"
                     /old_locus_tag="M1S26_20245"
     CDS             1308..1826
                     /locus_tag="M1S26_RS20245"
                     /old_locus_tag="M1S26_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000839337.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPVQKQSVKETVKVDSPTSAEVLLLRQQIDLLKNQLDDAKNRESFYQNQIETMQRLL
                     EAPKTNTTQSDDPGSTQAESTVEEKEDVIAMPPKESEVIENKRIPVPEHIEPEPEKRGF
                     WSRFFKPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            2036..2335
                     /locus_tag="M1S26_RS20250"
                     /old_locus_tag="M1S26_20250"
     CDS             2036..2335
                     /locus_tag="M1S26_RS20250"
                     /old_locus_tag="M1S26_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            2901..3257
                     /locus_tag="M1S26_RS20255"
                     /old_locus_tag="M1S26_20255"
     CDS             2901..3257
                     /locus_tag="M1S26_RS20255"
                     /old_locus_tag="M1S26_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269904.1"
                     /translation="MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSNKKRFYTEMLAIADEQ
                     YALHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3250..3552
                     /locus_tag="M1S26_RS20260"
                     /old_locus_tag="M1S26_20260"
     CDS             3250..3552
                     /locus_tag="M1S26_RS20260"
                     /old_locus_tag="M1S26_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001129974.1"
                     /translation="MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            3545..3754
                     /locus_tag="M1S26_RS20265"
                     /old_locus_tag="M1S26_20265"
     CDS             3545..3754
                     /locus_tag="M1S26_RS20265"
                     /old_locus_tag="M1S26_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069474.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCEYFS
                     HCKRPSYYIDIFDNAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(4080..4541)
                     /locus_tag="M1S26_RS20270"
                     /old_locus_tag="M1S26_20270"
     CDS             complement(4080..4541)
                     /locus_tag="M1S26_RS20270"
                     /old_locus_tag="M1S26_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(4846..7257)
                     /gene="znuD2"
                     /locus_tag="M1S26_RS20275"
                     /old_locus_tag="M1S26_20275"
     CDS             complement(4846..7257)
                     /gene="znuD2"
                     /locus_tag="M1S26_RS20275"
                     /old_locus_tag="M1S26_20275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            complement(7385..7699)
                     /locus_tag="M1S26_RS20280"
                     /old_locus_tag="M1S26_20280"
     CDS             complement(7385..7699)
                     /locus_tag="M1S26_RS20280"
                     /old_locus_tag="M1S26_20280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(7686..7973)
                     /locus_tag="M1S26_RS20285"
                     /old_locus_tag="M1S26_20285"
     CDS             complement(7686..7973)
                     /locus_tag="M1S26_RS20285"
                     /old_locus_tag="M1S26_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438827.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            complement(8241..8540)
                     /locus_tag="M1S26_RS20290"
                     /old_locus_tag="M1S26_20290"
     CDS             complement(8241..8540)
                     /locus_tag="M1S26_RS20290"
                     /old_locus_tag="M1S26_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            complement(8657..9418)
                     /locus_tag="M1S26_RS20295"
                     /old_locus_tag="M1S26_20295"
     CDS             complement(8657..9418)
                     /locus_tag="M1S26_RS20295"
                     /old_locus_tag="M1S26_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425105.1"
                     /translation="MEMEKAKKRGRPAQLLQIAELHAFVEFLEQQEQLSDLQSQVLKAL
                     NSVDCNFEGLTQTDQVLVKEALKPYREHLKLKLLFEELNNLPLKTEYEQKFLDLYELFQ
                     KNALDQMELNILKTLATRYLNFKAQKLEYSDLELYLSQLQKKDAGKKRKAENQRKFELG
                     GAVLVAFKKLNIDISNDTPQQITNRIVNTTKFHNEVRKSLIFKDVKTYENEYFKANKLF
                     IQVLEGLHTWQKGGELLSVIEIKKALEKGEE"
                     /name="hypothetical protein"
     gene            9723..10892
                     /locus_tag="M1S26_RS20300"
                     /old_locus_tag="M1S26_20300"
     CDS             9723..10892
                     /locus_tag="M1S26_RS20300"
                     /old_locus_tag="M1S26_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002134206.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIDFWQAADTYERANAKAYMEYEIALPNEFTSEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHEDYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENNPFSSLEVMYYLNYLIKNATNKDKLKNDLNEIIKNQGPILKQEAWSIYEMNEKLEDS
                     RRNSTPCFEFEVHSLPEYVLPIQNTPPKRTQSEPVQPKADDYDYGGPGF"
                     /name="MobA/MobL family protein"
     Typing          296..1230
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9802..10889
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tagcgaaaac atagggcaat ttttcattca ttgggctttt aattattttt aatcttttaa
       61 atgcttttaa ataagctgta aacctttatt tataatagtt tcatagttaa taaaactagg
      121 tttatcgacc cataaaacta ggtttatcga cccataaaac taggtttatc gacccataaa
      181 actaggttta tcgaccttaa agctaggttt atcgacccat aattaagtta atttacaact
      241 caattgaaat agtctattgt acaacctagt ttaattataa atttattatt agaaatgagt
      301 gaattaatcg taaaggataa tgctctaatt caagcaagtt ataccttaga tacagttgaa
      361 caaagactga tcctattagc cattgctgaa gctcgagaaa ctggacatgg gataactgaa
      421 aatagtcttt tagaagtaca tgcaagtagt tatataaata cttttaatgt cgagaaacat
      481 accgcttata ccgtactcag agaggcatct aaaagcttat ttgatcgcta tgtcacatac
      541 catgacatta atcctaagac aggtaaggat cgtagctttc attgtcgttg ggtcgacaaa
      601 attgggtatg aatctcaatc aggaattatt ttcctacgat tcacccaaga tatcgttcca
      661 ctcataactc gacttgaaga aaatttcact aaatatgaat tgcagcaggt ttctaggtta
      721 agtagctcat atgctattcg gctatacgag ctattaattc aatggagatc tgccggaaaa
      781 acgccacttt ttgatctatc tatctttaga caacaacttg gtgtcaaacc tcatcaatac
      841 aaaacaatga gtaactttaa aacatatgtt ttagattttg ctcttaagca ggtaaatgag
      901 ttaaccgata taacagctaa atatgagcaa cataaaaaag ggcgttctat ttcaggtttt
      961 tcattcactt tcaaacagaa aaaaatgagt aatctgccaa taaaaaataa gcgtgaccca
     1021 gacactatag atattttctc aaaaatgaca gatgctcaac gccatctgtt ttcccacaaa
     1081 ctgtcagaac ttcctgaaat gggaaagtat tctcatggta cagaaagcta tccgcaattc
     1141 gctgtacgca ttgcagaaat gcttcaaaac ccagaaaagt ttaaagaact ctatccttat
     1201 ctccaaaaag taggcttcaa agctgcatag aagatagact tgctgatttg tctattagac
     1261 agtctaatag acactaattt tttacaagat taggcagaga tttacacatg aaaagcctta
     1321 cagttcttga gctttctaag ctttacaata ttaatcgaca aaccatttac aacaatataa
     1381 agaaaggtat tttaagtaaa aattctgaca ataagattga tcttgcagaa gcgatccggg
     1441 tatttggtga gccagtacaa aaacagagtg taaaagaaac tgtaaaagta gacagtccaa
     1501 cttcggcaga agttttactc cttagacagc agatagacct actaaaaaat cagctagatg
     1561 atgcaaaaaa tagagaaagc ttctatcaaa atcaaattga aactatgcaa cgcctattgg
     1621 aagctccaaa aaccaataca acacaatctg atgatcctgg atcaacacag gcagaatcca
     1681 cagtagaaga aaaagaggac gtcatagcta tgcctcctaa ggagtcagag gttatcgaga
     1741 ataagcgtat tccagtccct gaacatattg agccagaacc agaaaaaagg ggcttctgga
     1801 gccgtttttt caagccctat ggatgatagg actatttaat cagactaatc aaaaccgtgg
     1861 aacaatgatc aataattgat cgtgaaacat aaaaaatcac taaaacagcc tgagcgtgac
     1921 tgcttaggct gtttttacat ccataagaca ttgttttaaa atgcttttta caaagaattt
     1981 cgtataaggt gtattatgtt aattttaggg aaacttaatt ctataggtga taccaatgga
     2041 caaaaagata gaagagaaaa ctaaagatat taatgaaaaa attcaaaaat tggatttagc
     2101 tattcaagaa ctaatagaaa ctacagacaa tttttttatt aatgataagt actatggaat
     2161 tgaatgtgct tttattttag accatgcact aaatattaaa atgcactttt tcaacaagta
     2221 tccagcattt gcaccacctc acttacgagg agtagaaaat tatgatcgaa ttatacatat
     2281 tagagaaaat aatcctgaag ataagctaga acaaatttct cctcttaaag actaaatttt
     2341 ctaatcttgt acttagagtc ccctactatt taacataatg gatattatgc gaaatggatc
     2401 tttaagatta ctttaagatc agtagcttac attgagtcat accctctgga acgacctcgt
     2461 tcaaccgact tagaaacaag tcggtttttt tatgctcaat tttgatactt cttgccaata
     2521 aaattgttta aaaaataatc aaaaatagca attccataga gtttataagc ttttttaccc
     2581 aaaagaatcc actatttctg tggataacca ctgggataaa ttttgattat tttttgtacg
     2641 ttttggctta cagcgtttca cgaaattgtc tgtatctatc ccccgatgaa gctcttatga
     2701 tcgatctata tggacaggat gttcaatgac ttaaaacaac aataataatc agtcaggcac
     2761 catgaccatc aggtagtcag ccctacctat gcgctccata ccgaaaaatc ccgacatgac
     2821 gtcgggattt tttattgcca tttgcaaagt cattcaatca gatttaatat atagactgta
     2881 atctatattg tgattgtaat atgtggacag tcattacaac ggatctgttt aatgaatggc
     2941 ttgaacagca agatgaagca acacaagaga aagtcttagc tgcattggtt gttctacaac
     3001 agcaagggcc tagtttaggt cgtcctttag tagataccgt ctacgattct aaatttacca
     3061 atatgaagga actacgtgtt cagcatcgtg gtaaaccgtt aagggcattt tttgcattcg
     3121 atcccctaag acaagcgatt gtgctgtgta ttggtgataa gagtaataaa aagcgttttt
     3181 atacagagat gctagcaatt gcagatgagc aatacgcact tcatctctca actttaggag
     3241 atcaatctaa tggctaagag cttacaagaa ttattggcta gtcgctctcc agagagtcaa
     3301 gctcgtattc aaaaaatggc tgatgaatta cttttagaaa gtcagcttca ccttatccga
     3361 gaagagttag aaatttccca gaaagaacta gctgcaaccc ttggaattaa acagccgtca
     3421 ttatcagcaa tcgaaaatcg tggtcatgat cttaaaattt caacgatgaa aaagtatgtt
     3481 gaagcaatgg gtggaaaact tcgtattgat gtagagcttc caacaggaaa acacataggg
     3541 ttcaatgtct aataataaac ctttgaaaaa tagtactaaa ttacttgtta acttagacaa
     3601 gtttatattt ttagtcaatg ctgccgatag cctagaagaa attgaaatta ttagagattt
     3661 atgctgtgaa tatttttcac attgtaagag gccaagctat tacattgata tttttgacaa
     3721 tgcttattgg ataaaatatt acgaaatagc ttaatccttc tagattcgca taagagattt
     3781 tatgttaaat gccgaaatga attaaaaata gaaatgtagt tgagctgatg cttgctcatt
     3841 tctttatttt tagtaattga ggtgaacata ctgtcagtac atggtagtta taagtaacgt
     3901 gtaactttgt ttgcacaaag atttgagtta tgccaagtga tggattgatt tgtacttaaa
     3961 attttaagct tatccacttg ctttgcaagc cactacactt tatttacaat cctatgttgt
     4021 ggaagtaagg gatcttgtac tcgcccagta tgttcaactt gattatatct aaaggactat
     4081 taggcttcaa tcaaatcaat tttccaatta gtcgactgaa ttaacacgtt taaatcacgt
     4141 aatttaagac taatatcatc agcttgtttt tgtaatgatg aaactggaat aactttatgc
     4201 cactttattt cccgcgtact ataacgatca gattcacgag atgtattact tattgctgca
     4261 gttagggttt tgtgctgttc tacaaattca tcacgcaaag tcaataaagc aagtagagaa
     4321 cggccatcat ttaattttgt taaagcatta gtttcatgaa ttgcataact taatttttga
     4381 aattcttgaa ttaaagaaaa tacttgtttt agtagttcat ttggatcttc agatggttga
     4441 tcaccatctt gtaccaatac atttgcattg atacgttgtt ttagtgaaat aatttttttt
     4501 tgttgatcac ttcgaagtaa gagagcttct gctaatttca tttttcacct attaaccaat
     4561 tagaattaag ttttaaacag cttatatcct cattctttaa agatcaaaaa aattgctttt
     4621 tatctagcta aaaggtgtct acaaaatcgg tggctattca gtgctgaaaa ggctgccgac
     4681 acataaagtg acccagattg aagagttact gccacactgc tggaaaccta aatcgaatta
     4741 aaaaataggt atgggattca gcggacgctt accttaatta ttaatctaat cattttataa
     4801 gaaaaatctt ctaagaaatt ttcttagaag attacagtat cttatctaga aattaagatt
     4861 taagcctaaa ctaaaatttc ttccaatttg gggaatatat ggcaaaaatg tttcatgggc
     4921 atagactttc tgatcaagta aattattcgc tttaaagaat aaatcatatt ctgtgtgaga
     4981 tagcttattt ttatatgcta aagtcatatt aagcatatta taacctgatg taacctgctc
     5041 aaatttagaa atattatctt gtttaaagac tcgataatat tctatttcac ctttaagcga
     5101 ttcatcaaaa tcagctttta gtcgtgtacc caaacgagca ggaggtaatc taggagtgta
     5161 acgatccttt tggggagcta aagtcggttc tctattccag atatcgtatg caattacagc
     5221 atcaggtaaa tttgttaaac ggcctttcac ataatctcca aaaagagagc cgtgataaac
     5281 agaattaaat tgatacccta tgttgccctc taatccataa aaattagcag cagactggct
     5341 ataatgatta atacgtagtg tatgctgatc tctaactttt gtagttccta aaacttcatt
     5401 gagtgtctgt aaatatatat agttatcaaa atcataatag taagtactga tttgataatc
     5461 aagtaaatca tccttatagg ccagactgat ttctaaatta ttggatttct cttttcttag
     5521 aaagcggtta cctatttcaa aagaattagt agcgagatgc ataccatgag tatacatttc
     5581 ttgtgcatta ggtaatcttt cttgatgtgt acctgttaat gataagatat aatttggagc
     5641 aaatctccag tggactgtac ctgcataaga aaaagctgtt tctttgttag gttgaacagc
     5701 ttctaaaata gattttaaat tttgagccct gattttatta tttttttcta cataaggact
     5761 gttatattta ttaggccaag gtttcattga atctttaatt ttttctatat cataatccat
     5821 actaactttc tgcttttcta tacgagcacc tagttcgaaa gtaaagtcgt tccagttata
     5881 tttttctagg ccaaatacac taaagttttt agtgacattt cgatttaata gctgctgatt
     5941 atctagatag gtggtatggc cttcttgaga atggactggt gataaagcac tattatcctg
     6001 ttccaaatat tgtagcccca agataccggt taattcccct aatggctgat gtgataactc
     6061 tatgcggcct acttttcctg tatttttaaa aaagttagta atcttatctc cctcgagttc
     6121 attgtgaaaa taatctatat aactcaatga ggtacgaatt ttgtcaatac cggtaaatgg
     6181 ttgagtgaat tctccacgca catcataacg tcgagtattt aaatcaatat aaggagcatt
     6241 atgagagaaa ttagtttcat gaatatgatt atgctgatga caatcaggac gtggatttat
     6301 gtaattaata tcttgctcat ccatcaattg aggataatat aaaagataat tttttaatcc
     6361 actaattcgt gaatcgatac cgattgcaga ggcaccacaa ccttcatata aatggctatg
     6421 ggctggtaaa ccatattcat catgacgatg tgtataagaa actcctaaat aagtattatc
     6481 gtctatccag gataatccaa gcgtacctac tctcgactct gcccagcttt ctggtaaata
     6541 ttttaaattt ttataaatac tgttttggtt ttcaagatct tctaaagaat tataagagct
     6601 agactggtaa tgaggagttt tatagttacc agcattacga tataaccctt ctgcccgtaa
     6661 agctacatgt gggcttaagc caactgttac acctgctgtg gtcaacttct cattactgcc
     6721 agtattaaaa cgtaatgtag tattaccctc taatccgtct tgaggcattt gggttggaat
     6781 tttatagtca atgacattga ctacccctgc cgcactattt gaactataca aaagggtact
     6841 tgcaccacgt ataatttcaa tagacttagc taaacttggt tcaactgtga cagcgtgatc
     6901 tggagacata ttagacatat ccaacacgtc agcattattc tgtaaaactt taatacgttt
     6961 tccttcctgc ccacgaataa taggagtaga tgctccacct ccatattgat tagaatgaat
     7021 tcctaattcc gtacccaagg catcccctag agttgtcgaa cgctgtttaa gtttactagt
     7081 atcaataaac tgattactaa catttagact tttgcctaac agctcagacc ctaactcatc
     7141 agattgtgca gttaatacta ttgtatttaa ggtagtaaca ggcccttcag ctgccaataa
     7201 aattttcgag gcccctaaag taattaaagc taaaaatagt ttacttttat ttagcataaa
     7261 acaaccaaat ttaatgttat attataatat aacacttaaa aatttataaa atctactatt
     7321 taaatttttg aatggtaatc ccccctaaaa atgaagtcaa aaatgggagc ctaagctccc
     7381 attcttattg atgatttttt aattctttta gcatagcatc tcgtaaaatt tcattcatac
     7441 gtgtttgata acctttgcct tgtgctttga accaagcaag tacatcagca tctaaccgaa
     7501 ttgaagtttg ttgctttact gggcggtaaa attgattttg gcgtacagca ttgctccagt
     7561 ctgtaatttc aggaatatct gatagatcta gctgatcatc aggaaccgtg cctttagcaa
     7621 gcaagcgttg aatttcagca tcttgcttct caccaaattt ctcattcagc tctttgtgtg
     7681 agtatctaac catgctcata tttgtttcgc tccgctttag tgacttgcct tgcactaatg
     7741 attcggatga tttcacagtc atcttcatca aaaatggtgt gagccaccaa taacattagt
     7801 acgcctttaa ctcgtccaat ggtttgccaa cgttcttcac cattagtata tctgtcttgg
     7861 attgagatcc gtaatggatc ttcaaaaaca aggcttgcag tttcgaaaga gatatcatgc
     7921 tttttctgat tctttcgatt cttagcctca tcccattcaa aatactgttc cataaaaaat
     7981 attcaaattt gtatatacaa taatgtatca cgaaattgta tcactcaagt tgagaagcgt
     8041 aatttttagt cagatttatt ggcaaaaact atcaaaatcg atcataaaaa agccgacttg
     8101 tttcaaagtc ggctttttgg gcatttttgg atttttatat ttttattaag tagttgatat
     8161 ttaaattaat atacaagtgt acttcgtata atatccatta tgttaaatag taggggactc
     8221 taagtacaag attagaaaat ttagtcttta agaggagaaa tttgttctag cttatcttca
     8281 ggattatttt ctctaatatg tataattcga tcataatttt ctactcctcg taagtgaggt
     8341 ggtgcaaatg ctggatactt gttgaaaaag tgcattttaa tatttagtgc atggtctaaa
     8401 ataaaagcac attcaattcc atagtactta tcattaataa aaaaattgtc tgtagtttct
     8461 attagttctt gaatagctaa atccaatttt tgaatttttt cattaatatc tttagttttc
     8521 tcttctatct ttttgtccat tggtatcacc tatagaatta agtttcccta aaattaacat
     8581 aatacacctt atgcgaaatt ccaatgtaag cccaaaatag aaatgtccaa cttctctaaa
     8641 atccctgcta aataaactac tcttcgcctt tttctaaggc ctttttaatt tcaattactg
     8701 acaataattc tccgcccttt tgccaggtat gtaatccttc taacacctga ataaaaagtt
     8761 tatttgcttt aaaatattca ttttcatatg tcttaacatc tttaaaaatt aaagatttac
     8821 gtacttcatt atgaaacttt gtcgtattca caatccgatt ggtaatctgt tgaggagtat
     8881 cattgctaat atcaatattg agtttcttaa atgcgacaag aacagcacca cctaattcaa
     8941 atttacgttg attttcagct ttgcgttttt tcccagcatc cttcttctgt agctgactca
     9001 aatacaattc taaatcactg tattcaagct tttgcgcttt aaaattcaga taccgtgtcg
     9061 ccaaggtttt taaaatattt agttccatct gatctagtgc attcttttga aaaagttcat
     9121 acagatctaa aaacttctgt tcatactcgg ttttaagagg cagattattc aactcttcaa
     9181 acagcaattt caacttcaga tgttcacgat aaggttttaa agcctctttg accagaacct
     9241 gatctgtctg agttagcccc tcaaaattac aatcaacact atttaaagcc tttaacactt
     9301 gagattgaag atcagaaagc tgttcttgct gttctaaaaa ctctacaaat gcatgtaact
     9361 cagcaatctg taacaactga gcaggtcgac cacgcttttt tgctttttcc atttccatca
     9421 tctgccgcca aattttcaga actgcaacct ctttaaatat ggttttacca tatttaaaga
     9481 gggcagtctg aaaataatta tttaatataa aaataaaaat tcttttcaag aaattttatt
     9541 tttatattaa ataattcgca cttgacagtt tctcctcatc ggagaaacta gtacgcaatg
     9601 gggcgcttcc cccttgaccc ccttcaaccg gatttttcag ccatacaggc atttcaaatc
     9661 cgggcggttt tcattccgtt tcactgcata aaaaccgaac ccacaaccca gcagaggtga
     9721 acatggccag ctaccacttc agcgtcaaaa gcaaaaataa gggctatgcg accggccatt
     9781 atctctacat ttcccggctg atgcagtatg aaggcatccg caaaaccagc aatgagacca
     9841 tagaacatat tgaacctggt cgatgtatgc ctgcctttgt aaaagatccc attgattttt
     9901 ggcaggcagc agatacgtat gaacgagcca atgccaaggc ctacatggag tatgaaattg
     9961 ccctaccgaa tgagttcacg tcagaacagc gtaaaacgct catagagacg tttttagaca
    10021 aacatatagt tccacagcaa tatccgcact catacgccat acacaacgta aaatcacgta
    10081 tcagtggcga agatcagcct cattgtcatc tgatgttctc gctaaaagcg gatgacggca
    10141 ttgaacgcag cgcagaacag tacttcaaac gctataaccc tcaagatcca gccaaaggcg
    10201 gtgccaaaaa aatccaactt caagatggcc atgaagacta ttccactttt ttgatctata
    10261 tccgaaaaca atgggaaaat catttgaatg atgcccttgc acagcactgc ccgaccgtca
    10321 cctacacact ggatggccaa gacatcacca tcaaaaatca ggtttcagca gacagctatg
    10381 aaaaatataa cgagatccac agcacgctat accttccaga gccaaaacta ggcgttggac
    10441 aacaaaatgc gacagcggaa tatctcgccc aaatccaaaa aatcagagaa cacaaccagc
    10501 aagaacgaga actcgaagaa cgccaaaagc aattagatca acaacatgaa tatttttata
    10561 gtgctgaaaa caacccattt tcatcattgg aagtcatgta ttacctgaat tacttaatca
    10621 agaatgcaac aaataaggac aaactaaaaa atgatttgaa tgaaattatc aaaaaccaag
    10681 gccctatact taaacaagaa gcttggtcca tctacgaaat gaatgaaaaa ctagaagact
    10741 caagacgtaa cagtacccca tgttttgagt tcgaggtgca ttcacttcct gaatacgtat
    10801 taccgataca aaatacgcct cccaaacgca ctcaatcaga accagtacaa cctaaagctg
    10861 atgattatga ttacggtggc cctggttttt aagctcagtt ggttttatcc aaagtttaca
    10921 gagcgagtgt ctacgagcga acagacacaa ttaaaatttt tgccatgctt tttccctgat
    10981 tacaaactcc cctttgctgc tgagactgag atacagagca tcccggaggg tgcgaacaga
    11041 tttaatttgc atctaggtcg agcgaagcga gtataaaaaa cttatgagcg aagcgaattc
    11101 cgagttgctt ttgctttttt gaagttatca cgagattaac ccaaaaattg ccccttcgaa
    11161 ctgagcgtaa gcgaagttca atagagtttg agcg
//
