LOCUS       NZ_CP096758            11194 bp    DNA     circular CON 21-MAY-2024
DEFINITION  Acinetobacter baumannii strain 5656 plasmid p5656, complete
            sequence.
ACCESSION   NZ_CP096758
VERSION     NZ_CP096758.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN24114649
            Assembly: GCF_023362175.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Gao,Y., Li,H. and Wang,H.
  TITLE     Origin, phylogeny, and transmission of the epidemic clone ST208 of
            carbapenem-resistant Acinetobacter baumannii on a global scale
  JOURNAL   Microbiol Spectr (2022) In press
REFERENCE   2  (bases 1 to 11194)
  AUTHORS   Gao,Y., Li,H. and Wang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2021) Department of Clinical Laboratory, Peking
            University People's Hospital, No.11 Xizhimen South Street, Beijing
            100044, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 268x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023362175.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 03:56:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,836
            CDSs (total)                      :: 3,743
            Genes (coding)                    :: 3,665
            CDSs (with protein)               :: 3,665
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 28 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 14 of 78
            Pseudo Genes (multiple problems)  :: 6 of 78
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096758.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11194
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="5656"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="p5656"
                     /geo_loc_name="China:Beijing"
                     /collection_date="2014-07-17"
                     /collected_by="Yue Gao"
     gene            163..1098
                     /gene="repM"
                     /locus_tag="M1S36_RS19155"
                     /old_locus_tag="M1S36_19155"
     CDS             163..1098
                     /gene="repM"
                     /locus_tag="M1S36_RS19155"
                     /old_locus_tag="M1S36_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001292329.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLREASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YESQSGIIFLRFTQDIVPLITRLEENFTKYELQQVSRLSSSYAIRLYELLIQWRSAGKT
                     PLFDLSIFRQQLGVKPHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFTFKQKKMSNLPIKNKRDPDTIDIFSKMTDAQRHLFSHKLSELPEMGKYSHGTESYPQ
                     FAVRIAEMLQNPEKFKELYPYLQKVGFKAA"
                     /name="repM"
     gene            1176..1694
                     /locus_tag="M1S36_RS19160"
                     /old_locus_tag="M1S36_19160"
     CDS             1176..1694
                     /locus_tag="M1S36_RS19160"
                     /old_locus_tag="M1S36_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000839337.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPVQKQSVKETVKVDSPTSAEVLLLRQQIDLLKNQLDDAKNRESFYQNQIETMQRLL
                     EAPKTNTTQSDDPGSTQAESTVEEKEDVIAMPPKESEVIENKRIPVPEHIEPEPEKRGF
                     WSRFFKPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            1904..2203
                     /locus_tag="M1S36_RS19165"
                     /old_locus_tag="M1S36_19165"
     CDS             1904..2203
                     /locus_tag="M1S36_RS19165"
                     /old_locus_tag="M1S36_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            2769..3125
                     /locus_tag="M1S36_RS19170"
                     /old_locus_tag="M1S36_19170"
     CDS             2769..3125
                     /locus_tag="M1S36_RS19170"
                     /old_locus_tag="M1S36_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269904.1"
                     /translation="MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSNKKRFYTEMLAIADEQ
                     YALHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3118..3420
                     /locus_tag="M1S36_RS19175"
                     /old_locus_tag="M1S36_19175"
     CDS             3118..3420
                     /locus_tag="M1S36_RS19175"
                     /old_locus_tag="M1S36_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001129974.1"
                     /translation="MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            3413..3622
                     /locus_tag="M1S36_RS19180"
                     /old_locus_tag="M1S36_19180"
     CDS             3413..3622
                     /locus_tag="M1S36_RS19180"
                     /old_locus_tag="M1S36_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069474.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCEYFS
                     HCKRPSYYIDIFDNAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(3948..4409)
                     /locus_tag="M1S36_RS19185"
                     /old_locus_tag="M1S36_19185"
     CDS             complement(3948..4409)
                     /locus_tag="M1S36_RS19185"
                     /old_locus_tag="M1S36_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(4714..7125)
                     /gene="znuD2"
                     /locus_tag="M1S36_RS19190"
                     /old_locus_tag="M1S36_19190"
     CDS             complement(4714..7125)
                     /gene="znuD2"
                     /locus_tag="M1S36_RS19190"
                     /old_locus_tag="M1S36_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            complement(7253..7567)
                     /locus_tag="M1S36_RS19195"
                     /old_locus_tag="M1S36_19195"
     CDS             complement(7253..7567)
                     /locus_tag="M1S36_RS19195"
                     /old_locus_tag="M1S36_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(7554..7841)
                     /locus_tag="M1S36_RS19200"
                     /old_locus_tag="M1S36_19200"
     CDS             complement(7554..7841)
                     /locus_tag="M1S36_RS19200"
                     /old_locus_tag="M1S36_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438827.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            complement(8109..8408)
                     /locus_tag="M1S36_RS19205"
                     /old_locus_tag="M1S36_19205"
     CDS             complement(8109..8408)
                     /locus_tag="M1S36_RS19205"
                     /old_locus_tag="M1S36_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            complement(8525..9286)
                     /locus_tag="M1S36_RS19210"
                     /old_locus_tag="M1S36_19210"
     CDS             complement(8525..9286)
                     /locus_tag="M1S36_RS19210"
                     /old_locus_tag="M1S36_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425105.1"
                     /translation="MEMEKAKKRGRPAQLLQIAELHAFVEFLEQQEQLSDLQSQVLKAL
                     NSVDCNFEGLTQTDQVLVKEALKPYREHLKLKLLFEELNNLPLKTEYEQKFLDLYELFQ
                     KNALDQMELNILKTLATRYLNFKAQKLEYSDLELYLSQLQKKDAGKKRKAENQRKFELG
                     GAVLVAFKKLNIDISNDTPQQITNRIVNTTKFHNEVRKSLIFKDVKTYENEYFKANKLF
                     IQVLEGLHTWQKGGELLSVIEIKKALEKGEE"
                     /name="hypothetical protein"
     gene            9591..10760
                     /locus_tag="M1S36_RS19215"
                     /old_locus_tag="M1S36_19215"
     CDS             9591..10760
                     /locus_tag="M1S36_RS19215"
                     /old_locus_tag="M1S36_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002134206.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIDFWQAADTYERANAKAYMEYEIALPNEFTSEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHEDYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENNPFSSLEVMYYLNYLIKNATNKDKLKNDLNEIIKNQGPILKQEAWSIYEMNEKLEDS
                     RRNSTPCFEFEVHSLPEYVLPIQNTPPKRTQSEPVQPKADDYDYGGPGF"
                     /name="MobA/MobL family protein"
     Typing          164..1098
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9670..10757
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taaaactagg tttatcgacc cataaaacta ggtttatcga cccataaaac taggtttatc
       61 gaccttaaag ctaggtttat cgacccataa ttaagttaat ttacaactca attgaaatag
      121 tctattgtac aacctagttt aattataaat ttattattag aaatgagtga attaatcgta
      181 aaggataatg ctctaattca agcaagttat accttagata cagttgaaca aagactgatc
      241 ctattagcca ttgctgaagc tcgagaaact ggacatggga taactgaaaa tagtctttta
      301 gaagtacatg caagtagtta tataaatact tttaatgtcg agaaacatac cgcttatacc
      361 gtactcagag aggcatctaa aagcttattt gatcgctatg tcacatacca tgacattaat
      421 cctaagacag gtaaggatcg tagctttcat tgtcgttggg tcgacaaaat tgggtatgaa
      481 tctcaatcag gaattatttt cctacgattc acccaagata tcgttccact cataactcga
      541 cttgaagaaa atttcactaa atatgaattg cagcaggttt ctaggttaag tagctcatat
      601 gctattcggc tatacgagct attaattcaa tggagatctg ccggaaaaac gccacttttt
      661 gatctatcta tctttagaca acaacttggt gtcaaacctc atcaatacaa aacaatgagt
      721 aactttaaaa catatgtttt agattttgct cttaagcagg taaatgagtt aaccgatata
      781 acagctaaat atgagcaaca taaaaaaggg cgttctattt caggtttttc attcactttc
      841 aaacagaaaa aaatgagtaa tctgccaata aaaaataagc gtgacccaga cactatagat
      901 attttctcaa aaatgacaga tgctcaacgc catctgtttt cccacaaact gtcagaactt
      961 cctgaaatgg gaaagtattc tcatggtaca gaaagctatc cgcaattcgc tgtacgcatt
     1021 gcagaaatgc ttcaaaaccc agaaaagttt aaagaactct atccttatct ccaaaaagta
     1081 ggcttcaaag ctgcatagaa gatagacttg ctgatttgtc tattagacag tctaatagac
     1141 actaattttt tacaagatta ggcagagatt tacacatgaa aagccttaca gttcttgagc
     1201 tttctaagct ttacaatatt aatcgacaaa ccatttacaa caatataaag aaaggtattt
     1261 taagtaaaaa ttctgacaat aagattgatc ttgcagaagc gatccgggta tttggtgagc
     1321 cagtacaaaa acagagtgta aaagaaactg taaaagtaga cagtccaact tcggcagaag
     1381 ttttactcct tagacagcag atagacctac taaaaaatca gctagatgat gcaaaaaata
     1441 gagaaagctt ctatcaaaat caaattgaaa ctatgcaacg cctattggaa gctccaaaaa
     1501 ccaatacaac acaatctgat gatcctggat caacacaggc agaatccaca gtagaagaaa
     1561 aagaggacgt catagctatg cctcctaagg agtcagaggt tatcgagaat aagcgtattc
     1621 cagtccctga acatattgag ccagaaccag aaaaaagggg cttctggagc cgttttttca
     1681 agccctatgg atgataggac tatttaatca gactaatcaa aaccgtggaa caatgatcaa
     1741 taattgatcg tgaaacataa aaaatcacta aaacagcctg agcgtgactg cttaggctgt
     1801 ttttacatcc ataagacatt gttttaaaat gctttttaca aagaatttcg tataaggtgt
     1861 attatgttaa ttttagggaa acttaattct ataggtgata ccaatggaca aaaagataga
     1921 agagaaaact aaagatatta atgaaaaaat tcaaaaattg gatttagcta ttcaagaact
     1981 aatagaaact acagacaatt tttttattaa tgataagtac tatggaattg aatgtgcttt
     2041 tattttagac catgcactaa atattaaaat gcactttttc aacaagtatc cagcatttgc
     2101 accacctcac ttacgaggag tagaaaatta tgatcgaatt atacatatta gagaaaataa
     2161 tcctgaagat aagctagaac aaatttctcc tcttaaagac taaattttct aatcttgtac
     2221 ttagagtccc ctactattta acataatgga tattatgcga aatggatctt taagattact
     2281 ttaagatcag tagcttacat tgagtcatac cctctggaac gacctcgttc aaccgactta
     2341 gaaacaagtc ggttttttta tgctcaattt tgatacttct tgccaataaa attgtttaaa
     2401 aaataatcaa aaatagcaat tccatagagt ttataagctt ttttacccaa aagaatccac
     2461 tatttctgtg gataaccact gggataaatt ttgattattt tttgtacgtt ttggcttaca
     2521 gcgtttcacg aaattgtctg tatctatccc ccgatgaagc tcttatgatc gatctatatg
     2581 gacaggatgt tcaatgactt aaaacaacaa taataatcag tcaggcacca tgaccatcag
     2641 gtagtcagcc ctacctatgc gctccatacc gaaaaatccc gacatgacgt cgggattttt
     2701 tattgccatt tgcaaagtca ttcaatcaga tttaatatat agactgtaat ctatattgtg
     2761 attgtaatat gtggacagtc attacaacgg atctgtttaa tgaatggctt gaacagcaag
     2821 atgaagcaac acaagagaaa gtcttagctg cattggttgt tctacaacag caagggccta
     2881 gtttaggtcg tcctttagta gataccgtct acgattctaa atttaccaat atgaaggaac
     2941 tacgtgttca gcatcgtggt aaaccgttaa gggcattttt tgcattcgat cccctaagac
     3001 aagcgattgt gctgtgtatt ggtgataaga gtaataaaaa gcgtttttat acagagatgc
     3061 tagcaattgc agatgagcaa tacgcacttc atctctcaac tttaggagat caatctaatg
     3121 gctaagagct tacaagaatt attggctagt cgctctccag agagtcaagc tcgtattcaa
     3181 aaaatggctg atgaattact tttagaaagt cagcttcacc ttatccgaga agagttagaa
     3241 atttcccaga aagaactagc tgcaaccctt ggaattaaac agccgtcatt atcagcaatc
     3301 gaaaatcgtg gtcatgatct taaaatttca acgatgaaaa agtatgttga agcaatgggt
     3361 ggaaaacttc gtattgatgt agagcttcca acaggaaaac acatagggtt caatgtctaa
     3421 taataaacct ttgaaaaata gtactaaatt acttgttaac ttagacaagt ttatattttt
     3481 agtcaatgct gccgatagcc tagaagaaat tgaaattatt agagatttat gctgtgaata
     3541 tttttcacat tgtaagaggc caagctatta cattgatatt tttgacaatg cttattggat
     3601 aaaatattac gaaatagctt aatccttcta gattcgcata agagatttta tgttaaatgc
     3661 cgaaatgaat taaaaataga aatgtagttg agctgatgct tgctcatttc tttattttta
     3721 gtaattgagg tgaacatact gtcagtacat ggtagttata agtaacgtgt aactttgttt
     3781 gcacaaagat ttgagttatg ccaagtgatg gattgatttg tacttaaaat tttaagctta
     3841 tccacttgct ttgcaagcca ctacacttta tttacaatcc tatgttgtgg aagtaaggga
     3901 tcttgtactc gcccagtatg ttcaacttga ttatatctaa aggactatta ggcttcaatc
     3961 aaatcaattt tccaattagt cgactgaatt aacacgttta aatcacgtaa tttaagacta
     4021 atatcatcag cttgtttttg taatgatgaa actggaataa ctttatgcca ctttatttcc
     4081 cgcgtactat aacgatcaga ttcacgagat gtattactta ttgctgcagt tagggttttg
     4141 tgctgttcta caaattcatc acgcaaagtc aataaagcaa gtagagaacg gccatcattt
     4201 aattttgtta aagcattagt ttcatgaatt gcataactta atttttgaaa ttcttgaatt
     4261 aaagaaaata cttgttttag tagttcattt ggatcttcag atggttgatc accatcttgt
     4321 accaatacat ttgcattgat acgttgtttt agtgaaataa tttttttttg ttgatcactt
     4381 cgaagtaaga gagcttctgc taatttcatt tttcacctat taaccaatta gaattaagtt
     4441 ttaaacagct tatatcctca ttctttaaag atcaaaaaaa ttgcttttta tctagctaaa
     4501 aggtgtctac aaaatcggtg gctattcagt gctgaaaagg ctgccgacac ataaagtgac
     4561 ccagattgaa gagttactgc cacactgctg gaaacctaaa tcgaattaaa aaataggtat
     4621 gggattcagc ggacgcttac cttaattatt aatctaatca ttttataaga aaaatcttct
     4681 aagaaatttt cttagaagat tacagtatct tatctagaaa ttaagattta agcctaaact
     4741 aaaatttctt ccaatttggg gaatatatgg caaaaatgtt tcatgggcat agactttctg
     4801 atcaagtaaa ttattcgctt taaagaataa atcatattct gtgtgagata gcttattttt
     4861 atatgctaaa gtcatattaa gcatattata acctgatgta acctgctcaa atttagaaat
     4921 attatcttgt ttaaagactc gataatattc tatttcacct ttaagcgatt catcaaaatc
     4981 agcttttagt cgtgtaccca aacgagcagg aggtaatcta ggagtgtaac gatccttttg
     5041 gggagctaaa gtcggttctc tattccagat atcgtatgca attacagcat caggtaaatt
     5101 tgttaaacgg cctttcacat aatctccaaa aagagagccg tgataaacag aattaaattg
     5161 ataccctatg ttgccctcta atccataaaa attagcagca gactggctat aatgattaat
     5221 acgtagtgta tgctgatctc taacttttgt agttcctaaa acttcattga gtgtctgtaa
     5281 atatatatag ttatcaaaat cataatagta agtactgatt tgataatcaa gtaaatcatc
     5341 cttataggcc agactgattt ctaaattatt ggatttctct tttcttagaa agcggttacc
     5401 tatttcaaaa gaattagtag cgagatgcat accatgagta tacatttctt gtgcattagg
     5461 taatctttct tgatgtgtac ctgttaatga taagatataa tttggagcaa atctccagtg
     5521 gactgtacct gcataagaaa aagctgtttc tttgttaggt tgaacagctt ctaaaataga
     5581 ttttaaattt tgagccctga ttttattatt tttttctaca taaggactgt tatatttatt
     5641 aggccaaggt ttcattgaat ctttaatttt ttctatatca taatccatac taactttctg
     5701 cttttctata cgagcaccta gttcgaaagt aaagtcgttc cagttatatt tttctaggcc
     5761 aaatacacta aagtttttag tgacatttcg atttaatagc tgctgattat ctagataggt
     5821 ggtatggcct tcttgagaat ggactggtga taaagcacta ttatcctgtt ccaaatattg
     5881 tagccccaag ataccggtta attcccctaa tggctgatgt gataactcta tgcggcctac
     5941 ttttcctgta tttttaaaaa agttagtaat cttatctccc tcgagttcat tgtgaaaata
     6001 atctatataa ctcaatgagg tacgaatttt gtcaataccg gtaaatggtt gagtgaattc
     6061 tccacgcaca tcataacgtc gagtatttaa atcaatataa ggagcattat gagagaaatt
     6121 agtttcatga atatgattat gctgatgaca atcaggacgt ggatttatgt aattaatatc
     6181 ttgctcatcc atcaattgag gataatataa aagataattt tttaatccac taattcgtga
     6241 atcgataccg attgcagagg caccacaacc ttcatataaa tggctatggg ctggtaaacc
     6301 atattcatca tgacgatgtg tataagaaac tcctaaataa gtattatcgt ctatccagga
     6361 taatccaagc gtacctactc tcgactctgc ccagctttct ggtaaatatt ttaaattttt
     6421 ataaatactg ttttggtttt caagatcttc taaagaatta taagagctag actggtaatg
     6481 aggagtttta tagttaccag cattacgata taacccttct gcccgtaaag ctacatgtgg
     6541 gcttaagcca actgttacac ctgctgtggt caacttctca ttactgccag tattaaaacg
     6601 taatgtagta ttaccctcta atccgtcttg aggcatttgg gttggaattt tatagtcaat
     6661 gacattgact acccctgccg cactatttga actatacaaa agggtacttg caccacgtat
     6721 aatttcaata gacttagcta aacttggttc aactgtgaca gcgtgatctg gagacatatt
     6781 agacatatcc aacacgtcag cattattctg taaaacttta atacgttttc cttcctgccc
     6841 acgaataata ggagtagatg ctccacctcc atattgatta gaatgaattc ctaattccgt
     6901 acccaaggca tcccctagag ttgtcgaacg ctgtttaagt ttactagtat caataaactg
     6961 attactaaca tttagacttt tgcctaacag ctcagaccct aactcatcag attgtgcagt
     7021 taatactatt gtatttaagg tagtaacagg cccttcagct gccaataaaa ttttcgaggc
     7081 ccctaaagta attaaagcta aaaatagttt acttttattt agcataaaac aaccaaattt
     7141 aatgttatat tataatataa cacttaaaaa tttataaaat ctactattta aatttttgaa
     7201 tggtaatccc ccctaaaaat gaagtcaaaa atgggagcct aagctcccat tcttattgat
     7261 gattttttaa ttcttttagc atagcatctc gtaaaatttc attcatacgt gtttgataac
     7321 ctttgccttg tgctttgaac caagcaagta catcagcatc taaccgaatt gaagtttgtt
     7381 gctttactgg gcggtaaaat tgattttggc gtacagcatt gctccagtct gtaatttcag
     7441 gaatatctga tagatctagc tgatcatcag gaaccgtgcc tttagcaagc aagcgttgaa
     7501 tttcagcatc ttgcttctca ccaaatttct cattcagctc tttgtgtgag tatctaacca
     7561 tgctcatatt tgtttcgctc cgctttagtg acttgccttg cactaatgat tcggatgatt
     7621 tcacagtcat cttcatcaaa aatggtgtga gccaccaata acattagtac gcctttaact
     7681 cgtccaatgg tttgccaacg ttcttcacca ttagtatatc tgtcttggat tgagatccgt
     7741 aatggatctt caaaaacaag gcttgcagtt tcgaaagaga tatcatgctt tttctgattc
     7801 tttcgattct tagcctcatc ccattcaaaa tactgttcca taaaaaatat tcaaatttgt
     7861 atatacaata atgtatcacg aaattgtatc actcaagttg agaagcgtaa tttttagtca
     7921 gatttattgg caaaaactat caaaatcgat cataaaaaag ccgacttgtt tcaaagtcgg
     7981 ctttttgggc atttttggat ttttatattt ttattaagta gttgatattt aaattaatat
     8041 acaagtgtac ttcgtataat atccattatg ttaaatagta ggggactcta agtacaagat
     8101 tagaaaattt agtctttaag aggagaaatt tgttctagct tatcttcagg attattttct
     8161 ctaatatgta taattcgatc ataattttct actcctcgta agtgaggtgg tgcaaatgct
     8221 ggatacttgt tgaaaaagtg cattttaata tttagtgcat ggtctaaaat aaaagcacat
     8281 tcaattccat agtacttatc attaataaaa aaattgtctg tagtttctat tagttcttga
     8341 atagctaaat ccaatttttg aattttttca ttaatatctt tagttttctc ttctatcttt
     8401 ttgtccattg gtatcaccta tagaattaag tttccctaaa attaacataa tacaccttat
     8461 gcgaaattcc aatgtaagcc caaaatagaa atgtccaact tctctaaaat ccctgctaaa
     8521 taaactactc ttcgcctttt tctaaggcct ttttaatttc aattactgac aataattctc
     8581 cgcccttttg ccaggtatgt aatccttcta acacctgaat aaaaagttta tttgctttaa
     8641 aatattcatt ttcatatgtc ttaacatctt taaaaattaa agatttacgt acttcattat
     8701 gaaactttgt cgtattcaca atccgattgg taatctgttg aggagtatca ttgctaatat
     8761 caatattgag tttcttaaat gcgacaagaa cagcaccacc taattcaaat ttacgttgat
     8821 tttcagcttt gcgttttttc ccagcatcct tcttctgtag ctgactcaaa tacaattcta
     8881 aatcactgta ttcaagcttt tgcgctttaa aattcagata ccgtgtcgcc aaggttttta
     8941 aaatatttag ttccatctga tctagtgcat tcttttgaaa aagttcatac agatctaaaa
     9001 acttctgttc atactcggtt ttaagaggca gattattcaa ctcttcaaac agcaatttca
     9061 acttcagatg ttcacgataa ggttttaaag cctctttgac cagaacctga tctgtctgag
     9121 ttagcccctc aaaattacaa tcaacactat ttaaagcctt taacacttga gattgaagat
     9181 cagaaagctg ttcttgctgt tctaaaaact ctacaaatgc atgtaactca gcaatctgta
     9241 acaactgagc aggtcgacca cgcttttttg ctttttccat ttccatcatc tgccgccaaa
     9301 ttttcagaac tgcaacctct ttaaatatgg ttttaccata tttaaagagg gcagtctgaa
     9361 aataattatt taatataaaa ataaaaattc ttttcaagaa attttatttt tatattaaat
     9421 aattcgcact tgacagtttc tcctcatcgg agaaactagt acgcaatggg gcgcttcccc
     9481 cttgaccccc ttcaaccgga tttttcagcc atacaggcat ttcaaatccg ggcggttttc
     9541 attccgtttc actgcataaa aaccgaaccc acaacccagc agaggtgaac atggccagct
     9601 accacttcag cgtcaaaagc aaaaataagg gctatgcgac cggccattat ctctacattt
     9661 cccggctgat gcagtatgaa ggcatccgca aaaccagcaa tgagaccata gaacatattg
     9721 aacctggtcg atgtatgcct gcctttgtaa aagatcccat tgatttttgg caggcagcag
     9781 atacgtatga acgagccaat gccaaggcct acatggagta tgaaattgcc ctaccgaatg
     9841 agttcacgtc agaacagcgt aaaacgctca tagagacgtt tttagacaaa catatagttc
     9901 cacagcaata tccgcactca tacgccatac acaacgtaaa atcacgtatc agtggcgaag
     9961 atcagcctca ttgtcatctg atgttctcgc taaaagcgga tgacggcatt gaacgcagcg
    10021 cagaacagta cttcaaacgc tataaccctc aagatccagc caaaggcggt gccaaaaaaa
    10081 tccaacttca agatggccat gaagactatt ccactttttt gatctatatc cgaaaacaat
    10141 gggaaaatca tttgaatgat gcccttgcac agcactgccc gaccgtcacc tacacactgg
    10201 atggccaaga catcaccatc aaaaatcagg tttcagcaga cagctatgaa aaatataacg
    10261 agatccacag cacgctatac cttccagagc caaaactagg cgttggacaa caaaatgcga
    10321 cagcggaata tctcgcccaa atccaaaaaa tcagagaaca caaccagcaa gaacgagaac
    10381 tcgaagaacg ccaaaagcaa ttagatcaac aacatgaata tttttatagt gctgaaaaca
    10441 acccattttc atcattggaa gtcatgtatt acctgaatta cttaatcaag aatgcaacaa
    10501 ataaggacaa actaaaaaat gatttgaatg aaattatcaa aaaccaaggc cctatactta
    10561 aacaagaagc ttggtccatc tacgaaatga atgaaaaact agaagactca agacgtaaca
    10621 gtaccccatg ttttgagttc gaggtgcatt cacttcctga atacgtatta ccgatacaaa
    10681 atacgcctcc caaacgcact caatcagaac cagtacaacc taaagctgat gattatgatt
    10741 acggtggccc tggtttttaa gctcagttgg ttttatccaa agtttacaga gcgagtgtct
    10801 acgagcgaac agacacaatt aaaatttttg ccatgctttt tccctgatta caaactcccc
    10861 tttgctgctg agactgagat acagagcatc ccggagggtg cgaacagatt taatttgcat
    10921 ctaggtcgag cgaagcgagt ataaaaaact tatgagcgaa gcgaattccg agttgctttt
    10981 gcttttttga agttatcacg agattaaccc aaaaattgcc ccttcgaact gagcgtaagc
    11041 gaagttcaat agagtttgag cgtagcgaaa acatagggca atttttcatt cattgggctt
    11101 ttaattattt ttaatctttt aaatgctttt aaataagctg taaaccttta tttataatag
    11161 tttcatagtt aataaaacta ggtttatcga ccca
//
