LOCUS       NZ_CP096750            11194 bp    DNA     circular CON 21-MAY-2024
DEFINITION  Acinetobacter baumannii strain 5666 plasmid p5666_1, complete
            sequence.
ACCESSION   NZ_CP096750
VERSION     NZ_CP096750.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN24114654
            Assembly: GCF_023362115.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Gao,Y., Li,H. and Wang,H.
  TITLE     Origin, phylogeny, and transmission of the epidemic clone ST208 of
            carbapenem-resistant Acinetobacter baumannii on a global scale
  JOURNAL   Microbiol Spectr (2022) In press
REFERENCE   2  (bases 1 to 11194)
  AUTHORS   Gao,Y., Li,H. and Wang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2021) Department of Clinical Laboratory, Peking
            University People's Hospital, No.11 Xizhimen South Street, Beijing
            100044, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 415x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023362115.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 04:03:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,988
            CDSs (total)                      :: 3,893
            Genes (coding)                    :: 3,811
            CDSs (with protein)               :: 3,811
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 26 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096750.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11194
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="5666"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="p5666_1"
                     /geo_loc_name="China:Xinjiang"
                     /collection_date="2016-03-17"
                     /collected_by="Yue Gao"
     gene            229..1164
                     /gene="repM"
                     /locus_tag="M1S28_RS18835"
                     /old_locus_tag="M1S28_18835"
     CDS             229..1164
                     /gene="repM"
                     /locus_tag="M1S28_RS18835"
                     /old_locus_tag="M1S28_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001292329.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLREASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YESQSGIIFLRFTQDIVPLITRLEENFTKYELQQVSRLSSSYAIRLYELLIQWRSAGKT
                     PLFDLSIFRQQLGVKPHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFTFKQKKMSNLPIKNKRDPDTIDIFSKMTDAQRHLFSHKLSELPEMGKYSHGTESYPQ
                     FAVRIAEMLQNPEKFKELYPYLQKVGFKAA"
                     /name="repM"
     gene            1242..1760
                     /locus_tag="M1S28_RS18840"
                     /old_locus_tag="M1S28_18840"
     CDS             1242..1760
                     /locus_tag="M1S28_RS18840"
                     /old_locus_tag="M1S28_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000839337.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPVQKQSVKETVKVDSPTSAEVLLLRQQIDLLKNQLDDAKNRESFYQNQIETMQRLL
                     EAPKTNTTQSDDPGSTQAESTVEEKEDVIAMPPKESEVIENKRIPVPEHIEPEPEKRGF
                     WSRFFKPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            1970..2269
                     /locus_tag="M1S28_RS18845"
                     /old_locus_tag="M1S28_18845"
     CDS             1970..2269
                     /locus_tag="M1S28_RS18845"
                     /old_locus_tag="M1S28_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            2537..2824
                     /locus_tag="M1S28_RS18850"
                     /old_locus_tag="M1S28_18850"
     CDS             2537..2824
                     /locus_tag="M1S28_RS18850"
                     /old_locus_tag="M1S28_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438827.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            2811..3125
                     /locus_tag="M1S28_RS18855"
                     /old_locus_tag="M1S28_18855"
     CDS             2811..3125
                     /locus_tag="M1S28_RS18855"
                     /old_locus_tag="M1S28_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            3253..5664
                     /gene="znuD2"
                     /locus_tag="M1S28_RS18860"
                     /old_locus_tag="M1S28_18860"
     CDS             3253..5664
                     /gene="znuD2"
                     /locus_tag="M1S28_RS18860"
                     /old_locus_tag="M1S28_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            5969..6430
                     /locus_tag="M1S28_RS18865"
                     /old_locus_tag="M1S28_18865"
     CDS             5969..6430
                     /locus_tag="M1S28_RS18865"
                     /old_locus_tag="M1S28_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(6756..6965)
                     /locus_tag="M1S28_RS18870"
                     /old_locus_tag="M1S28_18870"
     CDS             complement(6756..6965)
                     /locus_tag="M1S28_RS18870"
                     /old_locus_tag="M1S28_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069474.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCEYFS
                     HCKRPSYYIDIFDNAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(6958..7260)
                     /locus_tag="M1S28_RS18875"
                     /old_locus_tag="M1S28_18875"
     CDS             complement(6958..7260)
                     /locus_tag="M1S28_RS18875"
                     /old_locus_tag="M1S28_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001129974.1"
                     /translation="MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7253..7609)
                     /locus_tag="M1S28_RS18880"
                     /old_locus_tag="M1S28_18880"
     CDS             complement(7253..7609)
                     /locus_tag="M1S28_RS18880"
                     /old_locus_tag="M1S28_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269904.1"
                     /translation="MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSNKKRFYTEMLAIADEQ
                     YALHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8175..8474)
                     /locus_tag="M1S28_RS18885"
                     /old_locus_tag="M1S28_18885"
     CDS             complement(8175..8474)
                     /locus_tag="M1S28_RS18885"
                     /old_locus_tag="M1S28_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            complement(8591..9352)
                     /locus_tag="M1S28_RS18890"
                     /old_locus_tag="M1S28_18890"
     CDS             complement(8591..9352)
                     /locus_tag="M1S28_RS18890"
                     /old_locus_tag="M1S28_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425105.1"
                     /translation="MEMEKAKKRGRPAQLLQIAELHAFVEFLEQQEQLSDLQSQVLKAL
                     NSVDCNFEGLTQTDQVLVKEALKPYREHLKLKLLFEELNNLPLKTEYEQKFLDLYELFQ
                     KNALDQMELNILKTLATRYLNFKAQKLEYSDLELYLSQLQKKDAGKKRKAENQRKFELG
                     GAVLVAFKKLNIDISNDTPQQITNRIVNTTKFHNEVRKSLIFKDVKTYENEYFKANKLF
                     IQVLEGLHTWQKGGELLSVIEIKKALEKGEE"
                     /name="hypothetical protein"
     gene            9657..10826
                     /locus_tag="M1S28_RS18895"
                     /old_locus_tag="M1S28_18895"
     CDS             9657..10826
                     /locus_tag="M1S28_RS18895"
                     /old_locus_tag="M1S28_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002134206.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIDFWQAADTYERANAKAYMEYEIALPNEFTSEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHEDYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENNPFSSLEVMYYLNYLIKNATNKDKLKNDLNEIIKNQGPILKQEAWSIYEMNEKLEDS
                     RRNSTPCFEFEVHSLPEYVLPIQNTPPKRTQSEPVQPKADDYDYGGPGF"
                     /name="MobA/MobL family protein"
     Typing          230..1164
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9736..10823
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttaaataagc tgtaaacctt tatttataat agtttcatag ttaataaaac taggtttatc
       61 gacccataaa actaggttta tcgacccata aaactaggtt tatcgaccca taaaactagg
      121 tttatcgacc ttaaagctag gtttatcgac ccataattaa gttaatttac aactcaattg
      181 aaatagtcta ttgtacaacc tagtttaatt ataaatttat tattagaaat gagtgaatta
      241 atcgtaaagg ataatgctct aattcaagca agttatacct tagatacagt tgaacaaaga
      301 ctgatcctat tagccattgc tgaagctcga gaaactggac atgggataac tgaaaatagt
      361 cttttagaag tacatgcaag tagttatata aatactttta atgtcgagaa acataccgct
      421 tataccgtac tcagagaggc atctaaaagc ttatttgatc gctatgtcac ataccatgac
      481 attaatccta agacaggtaa ggatcgtagc tttcattgtc gttgggtcga caaaattggg
      541 tatgaatctc aatcaggaat tattttccta cgattcaccc aagatatcgt tccactcata
      601 actcgacttg aagaaaattt cactaaatat gaattgcagc aggtttctag gttaagtagc
      661 tcatatgcta ttcggctata cgagctatta attcaatgga gatctgccgg aaaaacgcca
      721 ctttttgatc tatctatctt tagacaacaa cttggtgtca aacctcatca atacaaaaca
      781 atgagtaact ttaaaacata tgttttagat tttgctctta agcaggtaaa tgagttaacc
      841 gatataacag ctaaatatga gcaacataaa aaagggcgtt ctatttcagg tttttcattc
      901 actttcaaac agaaaaaaat gagtaatctg ccaataaaaa ataagcgtga cccagacact
      961 atagatattt tctcaaaaat gacagatgct caacgccatc tgttttccca caaactgtca
     1021 gaacttcctg aaatgggaaa gtattctcat ggtacagaaa gctatccgca attcgctgta
     1081 cgcattgcag aaatgcttca aaacccagaa aagtttaaag aactctatcc ttatctccaa
     1141 aaagtaggct tcaaagctgc atagaagata gacttgctga tttgtctatt agacagtcta
     1201 atagacacta attttttaca agattaggca gagatttaca catgaaaagc cttacagttc
     1261 ttgagctttc taagctttac aatattaatc gacaaaccat ttacaacaat ataaagaaag
     1321 gtattttaag taaaaattct gacaataaga ttgatcttgc agaagcgatc cgggtatttg
     1381 gtgagccagt acaaaaacag agtgtaaaag aaactgtaaa agtagacagt ccaacttcgg
     1441 cagaagtttt actccttaga cagcagatag acctactaaa aaatcagcta gatgatgcaa
     1501 aaaatagaga aagcttctat caaaatcaaa ttgaaactat gcaacgccta ttggaagctc
     1561 caaaaaccaa tacaacacaa tctgatgatc ctggatcaac acaggcagaa tccacagtag
     1621 aagaaaaaga ggacgtcata gctatgcctc ctaaggagtc agaggttatc gagaataagc
     1681 gtattccagt ccctgaacat attgagccag aaccagaaaa aaggggcttc tggagccgtt
     1741 ttttcaagcc ctatggatga taggactatt taatcagact aatcaaaacc gtggaacaat
     1801 gatcaataat tgatcgtgaa acataaaaaa tcactaaaac agcctgagcg tgactgctta
     1861 ggctgttttt acatccataa gacattgttt taaaatgctt tttacaaaga atttcgtata
     1921 aggtgtatta tgttaatttt agggaaactt aattctatag gtgataccaa tggacaaaaa
     1981 gatagaagag aaaactaaag atattaatga aaaaattcaa aaattggatt tagctattca
     2041 agaactaata gaaactacag acaatttttt tattaatgat aagtactatg gaattgaatg
     2101 tgcttttatt ttagaccatg cactaaatat taaaatgcac tttttcaaca agtatccagc
     2161 atttgcacca cctcacttac gaggagtaga aaattatgat cgaattatac atattagaga
     2221 aaataatcct gaagataagc tagaacaaat ttctcctctt aaagactaaa ttttctaatc
     2281 ttgtacttag agtcccctac tatttaacat aatggatatt atacgaagta cacttgtata
     2341 ttaatttaaa tatcaactac ttaataaaaa tataaaaatc caaaaatgcc caaaaagccg
     2401 actttgaaac aagtcggctt ttttatgatc gattttgata gtttttgcca ataaatctga
     2461 ctaaaaatta cgcttctcaa cttgagtgat acaatttcgt gatacattat tgtatataca
     2521 aatttgaata ttttttatgg aacagtattt tgaatgggat gaggctaaga atcgaaagaa
     2581 tcagaaaaag catgatatct ctttcgaaac tgcaagcctt gtttttgaag atccattacg
     2641 gatctcaatc caagacagat atactaatgg tgaagaacgt tggcaaacca ttggacgagt
     2701 taaaggcgta ctaatgttat tggtggctca caccattttt gatgaagatg actgtgaaat
     2761 catccgaatc attagtgcaa ggcaagtcac taaagcggag cgaaacaaat atgagcatgg
     2821 ttagatactc acacaaagag ctgaatgaga aatttggtga gaagcaagat gctgaaattc
     2881 aacgcttgct tgctaaaggc acggttcctg atgatcagct agatctatca gatattcctg
     2941 aaattacaga ctggagcaat gctgtacgcc aaaatcaatt ttaccgccca gtaaagcaac
     3001 aaacttcaat tcggttagat gctgatgtac ttgcttggtt caaagcacaa ggcaaaggtt
     3061 atcaaacacg tatgaatgaa attttacgag atgctatgct aaaagaatta aaaaatcatc
     3121 aataagaatg ggagcttagg ctcccatttt tgacttcatt tttagggggg attaccattc
     3181 aaaaatttaa atagtagatt ttataaattt ttaagtgtta tattataata taacattaaa
     3241 tttggttgtt ttatgctaaa taaaagtaaa ctatttttag ctttaattac tttaggggcc
     3301 tcgaaaattt tattggcagc tgaagggcct gttactacct taaatacaat agtattaact
     3361 gcacaatctg atgagttagg gtctgagctg ttaggcaaaa gtctaaatgt tagtaatcag
     3421 tttattgata ctagtaaact taaacagcgt tcgacaactc taggggatgc cttgggtacg
     3481 gaattaggaa ttcattctaa tcaatatgga ggtggagcat ctactcctat tattcgtggg
     3541 caggaaggaa aacgtattaa agttttacag aataatgctg acgtgttgga tatgtctaat
     3601 atgtctccag atcacgctgt cacagttgaa ccaagtttag ctaagtctat tgaaattata
     3661 cgtggtgcaa gtaccctttt gtatagttca aatagtgcgg caggggtagt caatgtcatt
     3721 gactataaaa ttccaaccca aatgcctcaa gacggattag agggtaatac tacattacgt
     3781 tttaatactg gcagtaatga gaagttgacc acagcaggtg taacagttgg cttaagccca
     3841 catgtagctt tacgggcaga agggttatat cgtaatgctg gtaactataa aactcctcat
     3901 taccagtcta gctcttataa ttctttagaa gatcttgaaa accaaaacag tatttataaa
     3961 aatttaaaat atttaccaga aagctgggca gagtcgagag taggtacgct tggattatcc
     4021 tggatagacg ataatactta tttaggagtt tcttatacac atcgtcatga tgaatatggt
     4081 ttaccagccc atagccattt atatgaaggt tgtggtgcct ctgcaatcgg tatcgattca
     4141 cgaattagtg gattaaaaaa ttatctttta tattatcctc aattgatgga tgagcaagat
     4201 attaattaca taaatccacg tcctgattgt catcagcata atcatattca tgaaactaat
     4261 ttctctcata atgctcctta tattgattta aatactcgac gttatgatgt gcgtggagaa
     4321 ttcactcaac catttaccgg tattgacaaa attcgtacct cattgagtta tatagattat
     4381 tttcacaatg aactcgaggg agataagatt actaactttt ttaaaaatac aggaaaagta
     4441 ggccgcatag agttatcaca tcagccatta ggggaattaa ccggtatctt ggggctacaa
     4501 tatttggaac aggataatag tgctttatca ccagtccatt ctcaagaagg ccataccacc
     4561 tatctagata atcagcagct attaaatcga aatgtcacta aaaactttag tgtatttggc
     4621 ctagaaaaat ataactggaa cgactttact ttcgaactag gtgctcgtat agaaaagcag
     4681 aaagttagta tggattatga tatagaaaaa attaaagatt caatgaaacc ttggcctaat
     4741 aaatataaca gtccttatgt agaaaaaaat aataaaatca gggctcaaaa tttaaaatct
     4801 attttagaag ctgttcaacc taacaaagaa acagcttttt cttatgcagg tacagtccac
     4861 tggagatttg ctccaaatta tatcttatca ttaacaggta cacatcaaga aagattacct
     4921 aatgcacaag aaatgtatac tcatggtatg catctcgcta ctaattcttt tgaaataggt
     4981 aaccgctttc taagaaaaga gaaatccaat aatttagaaa tcagtctggc ctataaggat
     5041 gatttacttg attatcaaat cagtacttac tattatgatt ttgataacta tatatattta
     5101 cagacactca atgaagtttt aggaactaca aaagttagag atcagcatac actacgtatt
     5161 aatcattata gccagtctgc tgctaatttt tatggattag agggcaacat agggtatcaa
     5221 tttaattctg tttatcacgg ctctcttttt ggagattatg tgaaaggccg tttaacaaat
     5281 ttacctgatg ctgtaattgc atacgatatc tggaatagag aaccgacttt agctccccaa
     5341 aaggatcgtt acactcctag attacctcct gctcgtttgg gtacacgact aaaagctgat
     5401 tttgatgaat cgcttaaagg tgaaatagaa tattatcgag tctttaaaca agataatatt
     5461 tctaaatttg agcaggttac atcaggttat aatatgctta atatgacttt agcatataaa
     5521 aataagctat ctcacacaga atatgattta ttctttaaag cgaataattt acttgatcag
     5581 aaagtctatg cccatgaaac atttttgcca tatattcccc aaattggaag aaattttagt
     5641 ttaggcttaa atcttaattt ctagataaga tactgtaatc ttctaagaaa atttcttaga
     5701 agatttttct tataaaatga ttagattaat aattaaggta agcgtccgct gaatcccata
     5761 cctatttttt aattcgattt aggtttccag cagtgtggca gtaactcttc aatctgggtc
     5821 actttatgtg tcggcagcct tttcagcact gaatagccac cgattttgta gacacctttt
     5881 agctagataa aaagcaattt ttttgatctt taaagaatga ggatataagc tgtttaaaac
     5941 ttaattctaa ttggttaata ggtgaaaaat gaaattagca gaagctctct tacttcgaag
     6001 tgatcaacaa aaaaaaatta tttcactaaa acaacgtatc aatgcaaatg tattggtaca
     6061 agatggtgat caaccatctg aagatccaaa tgaactacta aaacaagtat tttctttaat
     6121 tcaagaattt caaaaattaa gttatgcaat tcatgaaact aatgctttaa caaaattaaa
     6181 tgatggccgt tctctacttg ctttattgac tttgcgtgat gaatttgtag aacagcacaa
     6241 aaccctaact gcagcaataa gtaatacatc tcgtgaatct gatcgttata gtacgcggga
     6301 aataaagtgg cataaagtta ttccagtttc atcattacaa aaacaagctg atgatattag
     6361 tcttaaatta cgtgatttaa acgtgttaat tcagtcgact aattggaaaa ttgatttgat
     6421 tgaagcctaa tagtccttta gatataatca agttgaacat actgggcgag tacaagatcc
     6481 cttacttcca caacatagga ttgtaaataa agtgtagtgg cttgcaaagc aagtggataa
     6541 gcttaaaatt ttaagtacaa atcaatccat cacttggcat aactcaaatc tttgtgcaaa
     6601 caaagttaca cgttacttat aactaccatg tactgacagt atgttcacct caattactaa
     6661 aaataaagaa atgagcaagc atcagctcaa ctacatttct atttttaatt catttcggca
     6721 tttaacataa aatctcttat gcgaatctag aaggattaag ctatttcgta atattttatc
     6781 caataagcat tgtcaaaaat atcaatgtaa tagcttggcc tcttacaatg tgaaaaatat
     6841 tcacagcata aatctctaat aatttcaatt tcttctaggc tatcggcagc attgactaaa
     6901 aatataaact tgtctaagtt aacaagtaat ttagtactat ttttcaaagg tttattatta
     6961 gacattgaac cctatgtgtt ttcctgttgg aagctctaca tcaatacgaa gttttccacc
     7021 cattgcttca acatactttt tcatcgttga aattttaaga tcatgaccac gattttcgat
     7081 tgctgataat gacggctgtt taattccaag ggttgcagct agttctttct gggaaatttc
     7141 taactcttct cggataaggt gaagctgact ttctaaaagt aattcatcag ccattttttg
     7201 aatacgagct tgactctctg gagagcgact agccaataat tcttgtaagc tcttagccat
     7261 tagattgatc tcctaaagtt gagagatgaa gtgcgtattg ctcatctgca attgctagca
     7321 tctctgtata aaaacgcttt ttattactct tatcaccaat acacagcaca atcgcttgtc
     7381 ttaggggatc gaatgcaaaa aatgccctta acggtttacc acgatgctga acacgtagtt
     7441 ccttcatatt ggtaaattta gaatcgtaga cggtatctac taaaggacga cctaaactag
     7501 gcccttgctg ttgtagaaca accaatgcag ctaagacttt ctcttgtgtt gcttcatctt
     7561 gctgttcaag ccattcatta aacagatccg ttgtaatgac tgtccacata ttacaatcac
     7621 aatatagatt acagtctata tattaaatct gattgaatga ctttgcaaat ggcaataaaa
     7681 aatcccgacg tcatgtcggg atttttcggt atggagcgca taggtagggc tgactacctg
     7741 atggtcatgg tgcctgactg attattattg ttgttttaag tcattgaaca tcctgtccat
     7801 atagatcgat cataagagct tcatcggggg atagatacag acaatttcgt gaaacgctgt
     7861 aagccaaaac gtacaaaaaa taatcaaaat ttatcccagt ggttatccac agaaatagtg
     7921 gattcttttg ggtaaaaaag cttataaact ctatggaatt gctatttttg attatttttt
     7981 aaacaatttt attggcaaga agtatcaaaa ttgagcataa aaaaaccgac ttgtttctaa
     8041 gtcggttgaa cgaggtcgtt ccagagggta tgactcaatg taagctactg atcttaaagt
     8101 aatcttaaag atccatttcg cataatatcc attatgttaa atagtagggg actctaagta
     8161 caagattaga aaatttagtc tttaagagga gaaatttgtt ctagcttatc ttcaggatta
     8221 ttttctctaa tatgtataat tcgatcataa ttttctactc ctcgtaagtg aggtggtgca
     8281 aatgctggat acttgttgaa aaagtgcatt ttaatattta gtgcatggtc taaaataaaa
     8341 gcacattcaa ttccatagta cttatcatta ataaaaaaat tgtctgtagt ttctattagt
     8401 tcttgaatag ctaaatccaa tttttgaatt ttttcattaa tatctttagt tttctcttct
     8461 atctttttgt ccattggtat cacctataga attaagtttc cctaaaatta acataataca
     8521 ccttatgcga aattccaatg taagcccaaa atagaaatgt ccaacttctc taaaatccct
     8581 gctaaataaa ctactcttcg cctttttcta aggccttttt aatttcaatt actgacaata
     8641 attctccgcc cttttgccag gtatgtaatc cttctaacac ctgaataaaa agtttatttg
     8701 ctttaaaata ttcattttca tatgtcttaa catctttaaa aattaaagat ttacgtactt
     8761 cattatgaaa ctttgtcgta ttcacaatcc gattggtaat ctgttgagga gtatcattgc
     8821 taatatcaat attgagtttc ttaaatgcga caagaacagc accacctaat tcaaatttac
     8881 gttgattttc agctttgcgt tttttcccag catccttctt ctgtagctga ctcaaataca
     8941 attctaaatc actgtattca agcttttgcg ctttaaaatt cagataccgt gtcgccaagg
     9001 tttttaaaat atttagttcc atctgatcta gtgcattctt ttgaaaaagt tcatacagat
     9061 ctaaaaactt ctgttcatac tcggttttaa gaggcagatt attcaactct tcaaacagca
     9121 atttcaactt cagatgttca cgataaggtt ttaaagcctc tttgaccaga acctgatctg
     9181 tctgagttag cccctcaaaa ttacaatcaa cactatttaa agcctttaac acttgagatt
     9241 gaagatcaga aagctgttct tgctgttcta aaaactctac aaatgcatgt aactcagcaa
     9301 tctgtaacaa ctgagcaggt cgaccacgct tttttgcttt ttccatttcc atcatctgcc
     9361 gccaaatttt cagaactgca acctctttaa atatggtttt accatattta aagagggcag
     9421 tctgaaaata attatttaat ataaaaataa aaattctttt caagaaattt tatttttata
     9481 ttaaataatt cgcacttgac agtttctcct catcggagaa actagtacgc aatggggcgc
     9541 ttcccccttg acccccttca accggatttt tcagccatac aggcatttca aatccgggcg
     9601 gttttcattc cgtttcactg cataaaaacc gaacccacaa cccagcagag gtgaacatgg
     9661 ccagctacca cttcagcgtc aaaagcaaaa ataagggcta tgcgaccggc cattatctct
     9721 acatttcccg gctgatgcag tatgaaggca tccgcaaaac cagcaatgag accatagaac
     9781 atattgaacc tggtcgatgt atgcctgcct ttgtaaaaga tcccattgat ttttggcagg
     9841 cagcagatac gtatgaacga gccaatgcca aggcctacat ggagtatgaa attgccctac
     9901 cgaatgagtt cacgtcagaa cagcgtaaaa cgctcataga gacgttttta gacaaacata
     9961 tagttccaca gcaatatccg cactcatacg ccatacacaa cgtaaaatca cgtatcagtg
    10021 gcgaagatca gcctcattgt catctgatgt tctcgctaaa agcggatgac ggcattgaac
    10081 gcagcgcaga acagtacttc aaacgctata accctcaaga tccagccaaa ggcggtgcca
    10141 aaaaaatcca acttcaagat ggccatgaag actattccac ttttttgatc tatatccgaa
    10201 aacaatggga aaatcatttg aatgatgccc ttgcacagca ctgcccgacc gtcacctaca
    10261 cactggatgg ccaagacatc accatcaaaa atcaggtttc agcagacagc tatgaaaaat
    10321 ataacgagat ccacagcacg ctataccttc cagagccaaa actaggcgtt ggacaacaaa
    10381 atgcgacagc ggaatatctc gcccaaatcc aaaaaatcag agaacacaac cagcaagaac
    10441 gagaactcga agaacgccaa aagcaattag atcaacaaca tgaatatttt tatagtgctg
    10501 aaaacaaccc attttcatca ttggaagtca tgtattacct gaattactta atcaagaatg
    10561 caacaaataa ggacaaacta aaaaatgatt tgaatgaaat tatcaaaaac caaggcccta
    10621 tacttaaaca agaagcttgg tccatctacg aaatgaatga aaaactagaa gactcaagac
    10681 gtaacagtac cccatgtttt gagttcgagg tgcattcact tcctgaatac gtattaccga
    10741 tacaaaatac gcctcccaaa cgcactcaat cagaaccagt acaacctaaa gctgatgatt
    10801 atgattacgg tggccctggt ttttaagctc agttggtttt atccaaagtt tacagagcga
    10861 gtgtctacga gcgaacagac acaattaaaa tttttgccat gctttttccc tgattacaaa
    10921 ctcccctttg ctgctgagac tgagatacag agcatcccgg agggtgcgaa cagatttaat
    10981 ttgcatctag gtcgagcgaa gcgagtataa aaaacttatg agcgaagcga attccgagtt
    11041 gcttttgctt ttttgaagtt atcacgagat taacccaaaa attgcccctt cgaactgagc
    11101 gtaagcgaag ttcaatagag tttgagcgta gcgaaaacat agggcaattt ttcattcatt
    11161 gggcttttaa ttatttttaa tcttttaaat gctt
//
