LOCUS       NZ_CP096741            11196 bp    DNA     circular CON 21-MAY-2024
DEFINITION  Acinetobacter baumannii strain 5672 plasmid p5672, complete
            sequence.
ACCESSION   NZ_CP096741
VERSION     NZ_CP096741.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN24114658
            Assembly: GCF_023362035.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11196)
  AUTHORS   Gao,Y., Li,H. and Wang,H.
  TITLE     Origin, phylogeny, and transmission of the epidemic clone ST208 of
            carbapenem-resistant Acinetobacter baumannii on a global scale
  JOURNAL   Microbiol Spectr (2022) In press
REFERENCE   2  (bases 1 to 11196)
  AUTHORS   Gao,Y., Li,H. and Wang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2021) Department of Clinical Laboratory, Peking
            University People's Hospital, No.11 Xizhimen South Street, Beijing
            100044, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 334x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023362035.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 03:52:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,821
            CDSs (total)                      :: 3,725
            Genes (coding)                    :: 3,623
            CDSs (with protein)               :: 3,623
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 45 of 102
            Pseudo Genes (incomplete)         :: 49 of 102
            Pseudo Genes (internal stop)      :: 14 of 102
            Pseudo Genes (multiple problems)  :: 6 of 102
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096741.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11196
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="5672"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="p5672"
                     /geo_loc_name="China:Shanghai"
                     /collection_date="2013-06-01"
                     /collected_by="Yue Gao"
     gene            189..1124
                     /gene="repM"
                     /locus_tag="M1S23_RS19085"
                     /old_locus_tag="M1S23_19085"
     CDS             189..1124
                     /gene="repM"
                     /locus_tag="M1S23_RS19085"
                     /old_locus_tag="M1S23_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001292329.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLREASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YESQSGIIFLRFTQDIVPLITRLEENFTKYELQQVSRLSSSYAIRLYELLIQWRSAGKT
                     PLFDLSIFRQQLGVKPHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFTFKQKKMSNLPIKNKRDPDTIDIFSKMTDAQRHLFSHKLSELPEMGKYSHGTESYPQ
                     FAVRIAEMLQNPEKFKELYPYLQKVGFKAA"
                     /name="repM"
     gene            1202..1720
                     /locus_tag="M1S23_RS19090"
                     /old_locus_tag="M1S23_19090"
     CDS             1202..1720
                     /locus_tag="M1S23_RS19090"
                     /old_locus_tag="M1S23_19090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000839337.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPVQKQSVKETVKVDSPTSAEVLLLRQQIDLLKNQLDDAKNRESFYQNQIETMQRLL
                     EAPKTNTTQSDDPGSTQAESTVEEKEDVIAMPPKESEVIENKRIPVPEHIEPEPEKRGF
                     WSRFFKPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            1930..2229
                     /locus_tag="M1S23_RS19095"
                     /old_locus_tag="M1S23_19095"
     CDS             1930..2229
                     /locus_tag="M1S23_RS19095"
                     /old_locus_tag="M1S23_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            2497..2784
                     /locus_tag="M1S23_RS19100"
                     /old_locus_tag="M1S23_19100"
     CDS             2497..2784
                     /locus_tag="M1S23_RS19100"
                     /old_locus_tag="M1S23_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438827.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            2771..3085
                     /locus_tag="M1S23_RS19105"
                     /old_locus_tag="M1S23_19105"
     CDS             2771..3085
                     /locus_tag="M1S23_RS19105"
                     /old_locus_tag="M1S23_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            3213..5624
                     /gene="znuD2"
                     /locus_tag="M1S23_RS19110"
                     /old_locus_tag="M1S23_19110"
     CDS             3213..5624
                     /gene="znuD2"
                     /locus_tag="M1S23_RS19110"
                     /old_locus_tag="M1S23_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            5929..6390
                     /locus_tag="M1S23_RS19115"
                     /old_locus_tag="M1S23_19115"
     CDS             5929..6390
                     /locus_tag="M1S23_RS19115"
                     /old_locus_tag="M1S23_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(6716..6925)
                     /locus_tag="M1S23_RS19120"
                     /old_locus_tag="M1S23_19120"
     CDS             complement(6716..6925)
                     /locus_tag="M1S23_RS19120"
                     /old_locus_tag="M1S23_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069474.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCEYFS
                     HCKRPSYYIDIFDNAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(6918..7220)
                     /locus_tag="M1S23_RS19125"
                     /old_locus_tag="M1S23_19125"
     CDS             complement(6918..7220)
                     /locus_tag="M1S23_RS19125"
                     /old_locus_tag="M1S23_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001129974.1"
                     /translation="MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7213..7569)
                     /locus_tag="M1S23_RS19130"
                     /old_locus_tag="M1S23_19130"
     CDS             complement(7213..7569)
                     /locus_tag="M1S23_RS19130"
                     /old_locus_tag="M1S23_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269904.1"
                     /translation="MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSNKKRFYTEMLAIADEQ
                     YALHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8135..8434)
                     /locus_tag="M1S23_RS19135"
                     /old_locus_tag="M1S23_19135"
     CDS             complement(8135..8434)
                     /locus_tag="M1S23_RS19135"
                     /old_locus_tag="M1S23_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            complement(8551..9314)
                     /locus_tag="M1S23_RS19140"
                     /old_locus_tag="M1S23_19140"
                     /pseudo=""
     CDS             complement(8551..9314)
                     /locus_tag="M1S23_RS19140"
                     /old_locus_tag="M1S23_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            9619..10788
                     /locus_tag="M1S23_RS19145"
                     /old_locus_tag="M1S23_19145"
     CDS             9619..10788
                     /locus_tag="M1S23_RS19145"
                     /old_locus_tag="M1S23_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002134206.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIDFWQAADTYERANAKAYMEYEIALPNEFTSEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHEDYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENNPFSSLEVMYYLNYLIKNATNKDKLKNDLNEIIKNQGPILKQEAWSIYEMNEKLEDS
                     RRNSTPCFEFEVHSLPEYVLPIQNTPPKRTQSEPVQPKADDYDYGGPGF"
                     /name="MobA/MobL family protein"
     Typing          190..1124
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9698..10785
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttaataaaac taggtttatc gacccataaa actaggttta tcgacccata aaactaggtt
       61 tatcgaccca taaaactagg tttatcgacc ttaaagctag gtttatcgac ccataattaa
      121 gttaatttac aactcaattg aaatagtcta ttgtacaacc tagtttaatt ataaatttat
      181 tattagaaat gagtgaatta atcgtaaagg ataatgctct aattcaagca agttatacct
      241 tagatacagt tgaacaaaga ctgatcctat tagccattgc tgaagctcga gaaactggac
      301 atgggataac tgaaaatagt cttttagaag tacatgcaag tagttatata aatactttta
      361 atgtcgagaa acataccgct tataccgtac tcagagaggc atctaaaagc ttatttgatc
      421 gctatgtcac ataccatgac attaatccta agacaggtaa ggatcgtagc tttcattgtc
      481 gttgggtcga caaaattggg tatgaatctc aatcaggaat tattttccta cgattcaccc
      541 aagatatcgt tccactcata actcgacttg aagaaaattt cactaaatat gaattgcagc
      601 aggtttctag gttaagtagc tcatatgcta ttcggctata cgagctatta attcaatgga
      661 gatctgccgg aaaaacgcca ctttttgatc tatctatctt tagacaacaa cttggtgtca
      721 aacctcatca atacaaaaca atgagtaact ttaaaacata tgttttagat tttgctctta
      781 agcaggtaaa tgagttaacc gatataacag ctaaatatga gcaacataaa aaagggcgtt
      841 ctatttcagg tttttcattc actttcaaac agaaaaaaat gagtaatctg ccaataaaaa
      901 ataagcgtga cccagacact atagatattt tctcaaaaat gacagatgct caacgccatc
      961 tgttttccca caaactgtca gaacttcctg aaatgggaaa gtattctcat ggtacagaaa
     1021 gctatccgca attcgctgta cgcattgcag aaatgcttca aaacccagaa aagtttaaag
     1081 aactctatcc ttatctccaa aaagtaggct tcaaagctgc atagaagata gacttgctga
     1141 tttgtctatt agacagtcta atagacacta attttttaca agattaggca gagatttaca
     1201 catgaaaagc cttacagttc ttgagctttc taagctttac aatattaatc gacaaaccat
     1261 ttacaacaat ataaagaaag gtattttaag taaaaattct gacaataaga ttgatcttgc
     1321 agaagcgatc cgggtatttg gtgagccagt acaaaaacag agtgtaaaag aaactgtaaa
     1381 agtagacagt ccaacttcgg cagaagtttt actccttaga cagcagatag acctactaaa
     1441 aaatcagcta gatgatgcaa aaaatagaga aagcttctat caaaatcaaa ttgaaactat
     1501 gcaacgccta ttggaagctc caaaaaccaa tacaacacaa tctgatgatc ctggatcaac
     1561 acaggcagaa tccacagtag aagaaaaaga ggacgtcata gctatgcctc ctaaggagtc
     1621 agaggttatc gagaataagc gtattccagt ccctgaacat attgagccag aaccagaaaa
     1681 aaggggcttc tggagccgtt ttttcaagcc ctatggatga taggactatt taatcagact
     1741 aatcaaaacc gtggaacaat gatcaataat tgatcgtgaa acataaaaaa tcactaaaac
     1801 agcctgagcg tgactgctta ggctgttttt acatccataa gacattgttt taaaatgctt
     1861 tttacaaaga atttcgtata aggtgtatta tgttaatttt agggaaactt aattctatag
     1921 gtgataccaa tggacaaaaa gatagaagag aaaactaaag atattaatga aaaaattcaa
     1981 aaattggatt tagctattca agaactaata gaaactacag acaatttttt tattaatgat
     2041 aagtactatg gaattgaatg tgcttttatt ttagaccatg cactaaatat taaaatgcac
     2101 tttttcaaca agtatccagc atttgcacca cctcacttac gaggagtaga aaattatgat
     2161 cgaattatac atattagaga aaataatcct gaagataagc tagaacaaat ttctcctctt
     2221 aaagactaaa ttttctaatc ttgtacttag agtcccctac tatttaacat aatggatatt
     2281 atacgaagta cacttgtata ttaatttaaa tatcaactac ttaataaaaa tataaaaatc
     2341 caaaaatgcc caaaaagccg actttgaaac aagtcggctt ttttatgatc gattttgata
     2401 gtttttgcca ataaatctga ctaaaaatta cgcttctcaa cttgagtgat acaatttcgt
     2461 gatacattat tgtatataca aatttgaata ttttttatgg aacagtattt tgaatgggat
     2521 gaggctaaga atcgaaagaa tcagaaaaag catgatatct ctttcgaaac tgcaagcctt
     2581 gtttttgaag atccattacg gatctcaatc caagacagat atactaatgg tgaagaacgt
     2641 tggcaaacca ttggacgagt taaaggcgta ctaatgttat tggtggctca caccattttt
     2701 gatgaagatg actgtgaaat catccgaatc attagtgcaa ggcaagtcac taaagcggag
     2761 cgaaacaaat atgagcatgg ttagatactc acacaaagag ctgaatgaga aatttggtga
     2821 gaagcaagat gctgaaattc aacgcttgct tgctaaaggc acggttcctg atgatcagct
     2881 agatctatca gatattcctg aaattacaga ctggagcaat gctgtacgcc aaaatcaatt
     2941 ttaccgccca gtaaagcaac aaacttcaat tcggttagat gctgatgtac ttgcttggtt
     3001 caaagcacaa ggcaaaggtt atcaaacacg tatgaatgaa attttacgag atgctatgct
     3061 aaaagaatta aaaaatcatc aataagaatg ggagcttagg ctcccatttt tgacttcatt
     3121 tttagggggg attaccattc aaaaatttaa atagtagatt ttataaattt ttaagtgtta
     3181 tattataata taacattaaa tttggttgtt ttatgctaaa taaaagtaaa ctatttttag
     3241 ctttaattac tttaggggcc tcgaaaattt tattggcagc tgaagggcct gttactacct
     3301 taaatacaat agtattaact gcacaatctg atgagttagg gtctgagctg ttaggcaaaa
     3361 gtctaaatgt tagtaatcag tttattgata ctagtaaact taaacagcgt tcgacaactc
     3421 taggggatgc cttgggtacg gaattaggaa ttcattctaa tcaatatgga ggtggagcat
     3481 ctactcctat tattcgtggg caggaaggaa aacgtattaa agttttacag aataatgctg
     3541 acgtgttgga tatgtctaat atgtctccag atcacgctgt cacagttgaa ccaagtttag
     3601 ctaagtctat tgaaattata cgtggtgcaa gtaccctttt gtatagttca aatagtgcgg
     3661 caggggtagt caatgtcatt gactataaaa ttccaaccca aatgcctcaa gacggattag
     3721 agggtaatac tacattacgt tttaatactg gcagtaatga gaagttgacc acagcaggtg
     3781 taacagttgg cttaagccca catgtagctt tacgggcaga agggttatat cgtaatgctg
     3841 gtaactataa aactcctcat taccagtcta gctcttataa ttctttagaa gatcttgaaa
     3901 accaaaacag tatttataaa aatttaaaat atttaccaga aagctgggca gagtcgagag
     3961 taggtacgct tggattatcc tggatagacg ataatactta tttaggagtt tcttatacac
     4021 atcgtcatga tgaatatggt ttaccagccc atagccattt atatgaaggt tgtggtgcct
     4081 ctgcaatcgg tatcgattca cgaattagtg gattaaaaaa ttatctttta tattatcctc
     4141 aattgatgga tgagcaagat attaattaca taaatccacg tcctgattgt catcagcata
     4201 atcatattca tgaaactaat ttctctcata atgctcctta tattgattta aatactcgac
     4261 gttatgatgt gcgtggagaa ttcactcaac catttaccgg tattgacaaa attcgtacct
     4321 cattgagtta tatagattat tttcacaatg aactcgaggg agataagatt actaactttt
     4381 ttaaaaatac aggaaaagta ggccgcatag agttatcaca tcagccatta ggggaattaa
     4441 ccggtatctt ggggctacaa tatttggaac aggataatag tgctttatca ccagtccatt
     4501 ctcaagaagg ccataccacc tatctagata atcagcagct attaaatcga aatgtcacta
     4561 aaaactttag tgtatttggc ctagaaaaat ataactggaa cgactttact ttcgaactag
     4621 gtgctcgtat agaaaagcag aaagttagta tggattatga tatagaaaaa attaaagatt
     4681 caatgaaacc ttggcctaat aaatataaca gtccttatgt agaaaaaaat aataaaatca
     4741 gggctcaaaa tttaaaatct attttagaag ctgttcaacc taacaaagaa acagcttttt
     4801 cttatgcagg tacagtccac tggagatttg ctccaaatta tatcttatca ttaacaggta
     4861 cacatcaaga aagattacct aatgcacaag aaatgtatac tcatggtatg catctcgcta
     4921 ctaattcttt tgaaataggt aaccgctttc taagaaaaga gaaatccaat aatttagaaa
     4981 tcagtctggc ctataaggat gatttacttg attatcaaat cagtacttac tattatgatt
     5041 ttgataacta tatatattta cagacactca atgaagtttt aggaactaca aaagttagag
     5101 atcagcatac actacgtatt aatcattata gccagtctgc tgctaatttt tatggattag
     5161 agggcaacat agggtatcaa tttaattctg tttatcacgg ctctcttttt ggagattatg
     5221 tgaaaggccg tttaacaaat ttacctgatg ctgtaattgc atacgatatc tggaatagag
     5281 aaccgacttt agctccccaa aaggatcgtt acactcctag attacctcct gctcgtttgg
     5341 gtacacgact aaaagctgat tttgatgaat cgcttaaagg tgaaatagaa tattatcgag
     5401 tctttaaaca agataatatt tctaaatttg agcaggttac atcaggttat aatatgctta
     5461 atatgacttt agcatataaa aataagctat ctcacacaga atatgattta ttctttaaag
     5521 cgaataattt acttgatcag aaagtctatg cccatgaaac atttttgcca tatattcccc
     5581 aaattggaag aaattttagt ttaggcttaa atcttaattt ctagataaga tactgtaatc
     5641 ttctaagaaa atttcttaga agatttttct tataaaatga ttagattaat aattaaggta
     5701 agcgtccgct gaatcccata cctatttttt aattcgattt aggtttccag cagtgtggca
     5761 gtaactcttc aatctgggtc actttatgtg tcggcagcct tttcagcact gaatagccac
     5821 cgattttgta gacacctttt agctagataa aaagcaattt ttttgatctt taaagaatga
     5881 ggatataagc tgtttaaaac ttaattctaa ttggttaata ggtgaaaaat gaaattagca
     5941 gaagctctct tacttcgaag tgatcaacaa aaaaaaatta tttcactaaa acaacgtatc
     6001 aatgcaaatg tattggtaca agatggtgat caaccatctg aagatccaaa tgaactacta
     6061 aaacaagtat tttctttaat tcaagaattt caaaaattaa gttatgcaat tcatgaaact
     6121 aatgctttaa caaaattaaa tgatggccgt tctctacttg ctttattgac tttgcgtgat
     6181 gaatttgtag aacagcacaa aaccctaact gcagcaataa gtaatacatc tcgtgaatct
     6241 gatcgttata gtacgcggga aataaagtgg cataaagtta ttccagtttc atcattacaa
     6301 aaacaagctg atgatattag tcttaaatta cgtgatttaa acgtgttaat tcagtcgact
     6361 aattggaaaa ttgatttgat tgaagcctaa tagtccttta gatataatca agttgaacat
     6421 actgggcgag tacaagatcc cttacttcca caacatagga ttgtaaataa agtgtagtgg
     6481 cttgcaaagc aagtggataa gcttaaaatt ttaagtacaa atcaatccat cacttggcat
     6541 aactcaaatc tttgtgcaaa caaagttaca cgttacttat aactaccatg tactgacagt
     6601 atgttcacct caattactaa aaataaagaa atgagcaagc atcagctcaa ctacatttct
     6661 atttttaatt catttcggca tttaacataa aatctcttat gcgaatctag aaggattaag
     6721 ctatttcgta atattttatc caataagcat tgtcaaaaat atcaatgtaa tagcttggcc
     6781 tcttacaatg tgaaaaatat tcacagcata aatctctaat aatttcaatt tcttctaggc
     6841 tatcggcagc attgactaaa aatataaact tgtctaagtt aacaagtaat ttagtactat
     6901 ttttcaaagg tttattatta gacattgaac cctatgtgtt ttcctgttgg aagctctaca
     6961 tcaatacgaa gttttccacc cattgcttca acatactttt tcatcgttga aattttaaga
     7021 tcatgaccac gattttcgat tgctgataat gacggctgtt taattccaag ggttgcagct
     7081 agttctttct gggaaatttc taactcttct cggataaggt gaagctgact ttctaaaagt
     7141 aattcatcag ccattttttg aatacgagct tgactctctg gagagcgact agccaataat
     7201 tcttgtaagc tcttagccat tagattgatc tcctaaagtt gagagatgaa gtgcgtattg
     7261 ctcatctgca attgctagca tctctgtata aaaacgcttt ttattactct tatcaccaat
     7321 acacagcaca atcgcttgtc ttaggggatc gaatgcaaaa aatgccctta acggtttacc
     7381 acgatgctga acacgtagtt ccttcatatt ggtaaattta gaatcgtaga cggtatctac
     7441 taaaggacga cctaaactag gcccttgctg ttgtagaaca accaatgcag ctaagacttt
     7501 ctcttgtgtt gcttcatctt gctgttcaag ccattcatta aacagatccg ttgtaatgac
     7561 tgtccacata ttacaatcac aatatagatt acagtctata tattaaatct gattgaatga
     7621 ctttgcaaat ggcaataaaa aatcccgacg tcatgtcggg atttttcggt atggagcgca
     7681 taggtagggc tgactacctg atggtcatgg tgcctgactg attattattg ttgttttaag
     7741 tcattgaaca tcctgtccat atagatcgat cataagagct tcatcggggg atagatacag
     7801 acaatttcgt gaaacgctgt aagccaaaac gtacaaaaaa taatcaaaat ttatcccagt
     7861 ggttatccac agaaatagtg gattcttttg ggtaaaaaag cttataaact ctatggaatt
     7921 gctatttttg attatttttt aaacaatttt attggcaaga agtatcaaaa ttgagcataa
     7981 aaaaaccgac ttgtttctaa gtcggttgaa cgaggtcgtt ccagagggta tgactcaatg
     8041 taagctactg atcttaaagt aatcttaaag atccatttcg cataatatcc attatgttaa
     8101 atagtagggg actctaagta caagattaga aaatttagtc tttaagagga gaaatttgtt
     8161 ctagcttatc ttcaggatta ttttctctaa tatgtataat tcgatcataa ttttctactc
     8221 ctcgtaagtg aggtggtgca aatgctggat acttgttgaa aaagtgcatt ttaatattta
     8281 gtgcatggtc taaaataaaa gcacattcaa ttccatagta cttatcatta ataaaaaaat
     8341 tgtctgtagt ttctattagt tcttgaatag ctaaatccaa tttttgaatt ttttcattaa
     8401 tatctttagt tttctcttct atctttttgt ccattggtat cacctataga attaagtttc
     8461 cctaaaatta acataataca ccttatgcga aattccaatg taagcccaaa atagaaatgt
     8521 ccaacttctc taaaatccct gctaaataaa ctactcttcg cctttttcta aggccttttt
     8581 aatttcaatt actgacaata attctccgcc cttttgccag ggtatgtaat ccttctaaca
     8641 cctgaataaa aagtttattt gctttaaaat attcattttc atatgtctta acatctttaa
     8701 aaattaaaga tttacgtact tcattatgaa actttgtcgt attcacaatc cgattggtaa
     8761 tctgttgagg agtatcattg ctaatatcaa atattgagtt tcttaaatgc gacaagaaca
     8821 gcaccaccta attcaaattt acgttgattt tcagctttgc gttttttccc agcatccttc
     8881 ttctgtagct gactcaaata caattctaaa tcactgtatt caagcttttg cgctttaaaa
     8941 ttcagatacc gtgtcgccaa ggtttttaaa atatttagtt ccatctgatc tagtgcattc
     9001 ttttgaaaaa gttcatacag atctaaaaac ttctgttcat actcggtttt aagaggcaga
     9061 ttattcaact cttcaaacag caatttcaac ttcagatgtt cacgataagg ttttaaagcc
     9121 tctttgacca gaacctgatc tgtctgagtt agcccctcaa aattacaatc aacactattt
     9181 aaagccttta acacttgaga ttgaagatca gaaagctgtt cttgctgttc taaaaactct
     9241 acaaatgcat gtaactcagc aatctgtaac aactgagcag gtcgaccacg cttttttgct
     9301 ttttccattt ccatcatctg ccgccaaatt ttcagaactg caacctcttt aaatatggtt
     9361 ttaccatatt taaagagggc agtctgaaaa taattattta atataaaaat aaaaattctt
     9421 ttcaagaaat tttattttta tattaaataa ttcgcacttg acagtttctc ctcatcggag
     9481 aaactagtac gcaatggggc gcttccccct tgaccccctt caaccggatt tttcagccat
     9541 acaggcattt caaatccggg cggttttcat tccgtttcac tgcataaaaa ccgaacccac
     9601 aacccagcag aggtgaacat ggccagctac cacttcagcg tcaaaagcaa aaataagggc
     9661 tatgcgaccg gccattatct ctacatttcc cggctgatgc agtatgaagg catccgcaaa
     9721 accagcaatg agaccataga acatattgaa cctggtcgat gtatgcctgc ctttgtaaaa
     9781 gatcccattg atttttggca ggcagcagat acgtatgaac gagccaatgc caaggcctac
     9841 atggagtatg aaattgccct accgaatgag ttcacgtcag aacagcgtaa aacgctcata
     9901 gagacgtttt tagacaaaca tatagttcca cagcaatatc cgcactcata cgccatacac
     9961 aacgtaaaat cacgtatcag tggcgaagat cagcctcatt gtcatctgat gttctcgcta
    10021 aaagcggatg acggcattga acgcagcgca gaacagtact tcaaacgcta taaccctcaa
    10081 gatccagcca aaggcggtgc caaaaaaatc caacttcaag atggccatga agactattcc
    10141 acttttttga tctatatccg aaaacaatgg gaaaatcatt tgaatgatgc ccttgcacag
    10201 cactgcccga ccgtcaccta cacactggat ggccaagaca tcaccatcaa aaatcaggtt
    10261 tcagcagaca gctatgaaaa atataacgag atccacagca cgctatacct tccagagcca
    10321 aaactaggcg ttggacaaca aaatgcgaca gcggaatatc tcgcccaaat ccaaaaaatc
    10381 agagaacaca accagcaaga acgagaactc gaagaacgcc aaaagcaatt agatcaacaa
    10441 catgaatatt tttatagtgc tgaaaacaac ccattttcat cattggaagt catgtattac
    10501 ctgaattact taatcaagaa tgcaacaaat aaggacaaac taaaaaatga tttgaatgaa
    10561 attatcaaaa accaaggccc tatacttaaa caagaagctt ggtccatcta cgaaatgaat
    10621 gaaaaactag aagactcaag acgtaacagt accccatgtt ttgagttcga ggtgcattca
    10681 cttcctgaat acgtattacc gatacaaaat acgcctccca aacgcactca atcagaacca
    10741 gtacaaccta aagctgatga ttatgattac ggtggccctg gtttttaagc tcagttggtt
    10801 ttatccaaag tttacagagc gagtgtctac gagcgaacag acacaattaa aatttttgcc
    10861 atgctttttc cctgattaca aactcccctt tgctgctgag actgagatac agagcatccc
    10921 ggagggtgcg aacagattta atttgcatct aggtcgagcg aagcgagtat aaaaaactta
    10981 tgagcgaagc gaattccgag ttgcttttgc ttttttgaag ttatcacgag attaacccaa
    11041 aaattgcccc ttcgaactga gcgtaagcga agttcaatag agtttgagcg tagcgaaaac
    11101 atagggcaat ttttcattca ttgggctttt aattattttt aatcttttaa atgcttttaa
    11161 ataagctgta aacctttatt tataatagtt tcatag
//
