LOCUS       NZ_CP096269             6156 bp    DNA     circular CON 14-MAY-2024
DEFINITION  Klebsiella quasipneumoniae strain KP20-558 plasmid pKP20-558-6,
            complete sequence.
ACCESSION   NZ_CP096269
VERSION     NZ_CP096269.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27737809
            Assembly: GCF_023205055.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Qin,S. and Peng,J.
  TITLE     Identification of tmexC2-tmexD2.3-toprJ2 gene clusters in Klebsiella
            Pneumoniae KP20-558
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   Qin,S. and Peng,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2022) Pharmaceutical Sciences, Zhengzhou
            University, 100 Science Avenue, Zhengzhou, Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics workbench v. 10.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023205055.1-RS_2024_05_14
            Annotation Date                   :: 05/14/2024 05:03:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,861
            Genes (coding)                    :: 5,668
            CDSs (with protein)               :: 5,668
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 70 of 193
            Pseudo Genes (incomplete)         :: 128 of 193
            Pseudo Genes (internal stop)      :: 36 of 193
            Pseudo Genes (multiple problems)  :: 35 of 193
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096269.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP20-558"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1463165"
                     /plasmid="pKP20-558-6"
                     /geo_loc_name="China"
                     /lat_lon="34.80 N 113.54 E"
                     /collection_date="2020"
                     /collected_by="zhengzhou university"
     gene            complement(join(5530..6156,1..477))
                     /locus_tag="M0M92_RS29850"
                     /old_locus_tag="M0M92_29860"
     CDS             complement(join(5530..6156,1..477))
                     /locus_tag="M0M92_RS29850"
                     /old_locus_tag="M0M92_29860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="WP_017901372.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEIYNKDGNKLDLYGKIDGLHYFS
                     DDKSVDGDQTYMRIGVKGETQINDQLTGYGQWEYNVQANNVEGSDKQSWTRLAFAGLKF
                     GDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRANGVATYRNSDFFGLVD
                     GLNFALQYQGKNGSVSGEGQTNNGRGWNKQNGDGFGTAITYDIWEGISAGFAYSHSKRT
                     DDQNAAGLVGRGKYAETYTGGLKYDANNIYLAAEYTQTYNATRFSGDSVSGYANKAQNF
                     EVVAQYQFDFGLRPSVAYLQSKGKDIEGFGDQDLLKYVDVAATYYFNKNMSAYVDYKIN
                     LVDDNRFTSAAGISTDDIVALGLVYQF"
                     /name="porin OmpC"
     gene            <701..790
                     /locus_tag="M0M92_RS29855"
                     /old_locus_tag="M0M92_29865"
                     /pseudo=""
     CDS             <701..790
                     /locus_tag="M0M92_RS29855"
                     /old_locus_tag="M0M92_29865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901096.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /name="DinI-like family protein"
     gene            complement(806..991)
                     /locus_tag="M0M92_RS29860"
                     /old_locus_tag="M0M92_29870"
     CDS             complement(806..991)
                     /locus_tag="M0M92_RS29860"
                     /old_locus_tag="M0M92_29870"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_017901373.1"
                     /translation="MSRVTAIISAVVMLNGERVDDDRLQLDTNPLLMDAISAVKYLAGK
                     MEQLQNIIAELKASNA"
                     /name="hypothetical protein"
     gene            complement(1080..>1440)
                     /locus_tag="M0M92_RS29865"
                     /old_locus_tag="M0M92_29875"
                     /pseudo=""
     CDS             complement(1080..>1440)
                     /locus_tag="M0M92_RS29865"
                     /old_locus_tag="M0M92_29875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383026.1"
                     /GO_function="GO:0003796 - lysozyme activity [Evidence
                     IEA]"
                     /GO_process="GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]; GO:0009253 - peptidoglycan
                     catabolic process [Evidence IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /name="glycoside hydrolase family protein"
     gene            complement(1516..2547)
                     /locus_tag="M0M92_RS29870"
                     /old_locus_tag="M0M92_29880"
     CDS             complement(1516..2547)
                     /locus_tag="M0M92_RS29870"
                     /old_locus_tag="M0M92_29880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element ISEc33 family transposase"
                     /protein_id="WP_001752311.1"
                     /translation="MPIIVPIPRGERRLMQKAIHKTRDKNHARRLTAMLMLHRGERVSD
                     VARTLCCARSSVGRWINWFTHSGIEGLKSLPAGRSRRWPFEHICTLLRELIKHSPGDFG
                     YQRSRWSTELLAIKINEITGCQLHAGTVRRWLPSAGLVWRRAAPTLRIRDPHKDEKMAV
                     IHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGALHSG
                     TGKVSYVGGNSKSSALFIALLKHLKATYRRAKTITLIVDNYIIHKSRETQRWLKANPKF
                     RVIYQPVYSPWVNHVEQLWQALHDTITRNHQCRSMWQLLKKVRHFMETASPFPGGKHGQ
                     AKV"
                     /name="IS630-like element ISEc33 family transposase"
     gene            2798..3778
                     /locus_tag="M0M92_RS29875"
                     /old_locus_tag="M0M92_29885"
     CDS             2798..3778
                     /locus_tag="M0M92_RS29875"
                     /old_locus_tag="M0M92_29885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.2"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="WP_042934497.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRRFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKASIRAKVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
                     /name="IS5-like element IS5 family transposase"
     gene            <3925..4548
                     /gene="aqpZ"
                     /locus_tag="M0M92_RS29880"
                     /old_locus_tag="M0M92_29890"
                     /pseudo=""
     CDS             <3925..4548
                     /gene="aqpZ"
                     /locus_tag="M0M92_RS29880"
                     /old_locus_tag="M0M92_29890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226086.1"
                     /GO_function="GO:0015250 - water channel activity [Evidence
                     IEA]"
                     /GO_process="GO:0006833 - water transport [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /name="aqpZ"
     gene            complement(4758..5471)
                     /locus_tag="M0M92_RS29885"
                     /old_locus_tag="M0M92_29895"
     CDS             complement(4758..5471)
                     /locus_tag="M0M92_RS29885"
                     /old_locus_tag="M0M92_29895"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_071600436.1"
                     /translation="MMDNKPLNKKELESIFLTSGAFNPKGLYLGEGFRKQALELGFPPY
                     ILPTEINIVLHYSPELSVRTFVSTLWNTGASLTEVIHLERKSFVFKSPRPYVKVLSSET
                     ESLSLDNDNRLSPRRKIPLLSPYYIAQVKLLIATLKIAGNTCGDSTKPRRIWDVDNFIA
                     TSWIDNAIKHARLDGVIFPVEVTPDAIKHAFAMNMLILGVEPSIVMKLMGFKRNKWLEI
                     YLKVKCLIMSLERDM"
                     /name="tyrosine-type recombinase/integrase"
ORIGIN
        1 gaagaagtca gagttacgat aggttgcaac accattagca cgctgctgca tgaagttgtc
       61 agagccataa gtgtcaccgc caaactctgg cagaacgtca gtccaggaag ttacgtcgta
      121 gacaacgccg tagttacgac cgtagtcgaa agagcccacg tcgccgaatt tcagaccagc
      181 aaatgccaga cgagtccagg actgcttgtc agaaccttca acgttgttcg cctgaacgtt
      241 gtattcccac tgaccgtagc cagtcagttg gtcattaatc tgggtttcgc ccttcacgcc
      301 gatacgcatg taggtctgat cgccatcgac gctcttgtca tcagaaaagt aatgcaagcc
      361 atcaatttta ccgtacaggt ctaatttgtt gccatctttg ttataaattt cagccgcatt
      421 tgctgcacct gctaccagca gagccggtac caagagggat agtactttaa ctttcatggt
      481 tgaaccctcg ttttatattt gccactgcgt atcgggctgt cattgtataa ttatctatac
      541 acaaaaaaat ccagccttct catgctgtaa gctagttaaa agttccctgt gaatacacct
      601 acattttgta acatattgta aaaggaatgc ataacacgtc atctcgtgtc tcataaccaa
      661 aacacatagc aataggaatg gacatgccct gaggggctat acagatgcca gtaagcatga
      721 aaaagaacaa attggcagag ttatcctgga gatgttcgaa gaggctgata tgtggatgtc
      781 tggagaataa aattagcgat accaatcatg catttgatgc cttcagctct gcaatgatgt
      841 tttgcaattg ctccattttg ccagctaaat atttcaccgc gctgatcgca tccatcagca
      901 gcggattggt atcgagttgc aagcgatcat cgtccactct ctcgccgttg agcataacca
      961 cagcgctgat aatggcggtc acgcggctca tgacatgatt attccaacag ccagaaacca
     1021 cggccacgca tcattaccat ctaaggcaag aaacgcagca atgacgaagc aaatcagatt
     1081 catttcacac catccagaca gagctgcttt tccgctgcgc gacgagtcac caggccggga
     1141 aggatttttc ctccgtcgta taccccagcg tgggaactgg ttgcaggcac tagttatctt
     1201 tccctgtcgc aggtaattaa acagagttga tttctgcata ctggcgcaac cagcgttaaa
     1261 cgtgatcgac gttacagcag aaaatgtatt gtcgcttagt tgtcgtccat tgccgtaggt
     1321 attgacgcag cgctcagccg caaggatgtt tcgctcccgg tctgcggcta tctgctaatc
     1381 agttttgcac acaccgcctt taacgtcatg cgtgttaagg cacagccctg atgtaggtag
     1441 tgattatgct aatagtaacg gacataaaaa agcctgaatc gccacggata atctaaatag
     1501 ctgcgcctaa taccgctaca cttttgcctg accatgtttt cctccgggga atgggctggc
     1561 ggtttccata aaatggcgga cctttttcag taactgccac attgagcggc actgatgatt
     1621 acgggttatc gtgtcatgaa gtgcctgcca tagctgttcc acatgattca cccacggcga
     1681 gtaaaccggc tggtaaatta ccctgaactt gggatttgct ttcaaccagc gctgtgtttc
     1741 gcggctttta tggataatgt agttatcaac gatcagcgtg attgttttcg cccgccgata
     1801 agtggctttc aggtgcttca gcagagcgat aaacagcgct gaacttttgc tgttgccgcc
     1861 cacgtagctg actttacccg tgccactgtg cagtgcgccg gccagatagt atttttcgtt
     1921 ctgccccggc gtcactaccc gtttctgctg tccgcgcaac tgccagtccg caccgatttt
     1981 aggattaagg tggatatcca cttcatcttc ataaaatacc ggatgctctg cgctgcattc
     2041 atccagcgct ttgtggatta ccgccatctt ttcatcttta tgtgggtcac ggatacgcag
     2101 agttggcgcg gccctgcgcc atacaagccc cgcagatggc aaccagcggc gaacggttcc
     2161 tgcatgtaac tggcaaccgg ttatctcatt gatttttatt gccagtaatt cggtgctcca
     2221 gcgtgaacgt tgataaccaa aatcgccggg agaatgcttt atcagctcac gtaacagggt
     2281 gcagatatgt tcaaaaggcc agcgtcggga gcgccctgcg ggtaaggatt tcaggccttc
     2341 aatacctgag tgcgtaaacc agttaatcca gcgaccaacg gatgaacggg cacaacagag
     2401 agttctggca acatcgctga cccgttcacc ccgatgaagc atcagcatgg ccgtgagtct
     2461 gcgggcatga tttttatcac gcgttttatg aatagctttc tgcatcaggc gtcgttcgcc
     2521 acggggtatt ggtactatga tcggcatcgc tcagtccggt tggtgatttt ggttggtttg
     2581 gcgattgatc agatcgcaca atccgggctg agttcccttt cagtgatcta ctattccgcg
     2641 cagctattta gacacttccg agccgttgat aatactggtt ttcatattct gtcggtgaca
     2701 tatgatcgct agaaccatgc cgacgcttag gaaggtgcga acaagtccct gatatgagat
     2761 catgtttgtc atctggagcc atggaacagg gttcatcatg agtcatcaac ttaccttcgc
     2821 cgacagtgaa ttcagcagta agcgccgtca gaccagaaaa gagattttct tgtcccgtat
     2881 ggagcagatt ctgccatggc aaaacatggt ggaagtcatc gagccgtttt accccaaggc
     2941 tggtaatggc cggcgacctt atccgctgga aaccatgcta cgcattcact gcatgcagca
     3001 ttggtacaac ctgagcgatg gcgcgatgga agatgctctg tacgaaatcg cctccatgcg
     3061 tcggtttgcc cggttatccc tggatagcgc cttgcctgac cgcaccacca tcatgaattt
     3121 ccgccacctg ctggagcagc atcaactggc ccgccaattg ttcaagacca tcaatcgctg
     3181 gctggccgaa gcaggcgtca tgatgactca aggcaccttg gtcgatgcca ccatcattga
     3241 ggcacccagc tcgaccaaga acaaagagca gcaacgcgat ccggagatgc atcagaccaa
     3301 gaaaggcaat cagtggcact ttggcatgaa ggcccacatt ggtgtcgatg ccaagagtgg
     3361 cctgacccac agcctagtca ccaccgcggc caacgagcat gacctcaatc agctgggtaa
     3421 tctgctgcat ggagaggagc aatttgtctc agccgatgcc ggctaccaag gggcgccaca
     3481 gcgcgaggag ctggccgagg tggatgtgga ctggctgatc gccgagcgcc ccggcaaggt
     3541 aagaaccttg aaacagcatc cacgcaagaa caaaacggcc atcaacatcg aatacatgaa
     3601 agccagcatc cgggccaagg tggagcaccc atttcgcatc atcaagcgac agttcggctt
     3661 cgtgaaagcc agatacaagg ggttgctgaa aaacgataac caactggcga tgttattcac
     3721 gctggccaac ctgtttcggg cggaccaaat gatacgtcag tgggagagat ctcactaaaa
     3781 actggggata acgccttaaa tggcgaagaa acggtctaaa taggctgatt caaggcattt
     3841 acgggagaaa aaatcggctc aaacatgaag aaatgaaatg actgagtcag ccgagaagaa
     3901 tttccccgct tattcgcacc ttcccttgcg gcagcattcc cgggattagg aattggtttt
     3961 gctggggtgg cgctggcctt cggtttaacg gtattaacca tggcatatgc cgtcggtcat
     4021 atttccggcg gacattttaa cccggcagtg actttaggtt tatgggctgg aggtcgtttc
     4081 ccggccaaag acgtaattgg ttatattatc gcccaagtcg tcggcggtat tatcgccgcc
     4141 gctattctgt atgttgtcgc cagcggtaaa gcgggcttcg acgccaccgc tagcgggttc
     4201 gcttcgaacg gttatggcga acactctccg ggcggcttct caatgctttc cgccattgtg
     4261 atagagattg tcctgacatg tggcttcctg ctggttattc acggttccac ggataaaagc
     4321 gctcctgccg gcttcgcccc tatcgccatt ggactggcac tgacgcttat tcacctgatc
     4381 agcattccgg tcaccaacac gtcggtaaac ccggcgcgta gcactgcggt agcgattttt
     4441 cagggcggtt gggcattgca acaattatgg ttattctggg tcatgccaat cattggtggg
     4501 atactgggcg gtttaattta tcgcactttg ctcagcagtg ataaatgaat aattaaaaac
     4561 tctatacgac tgcagatcat tccatattca cgccagccat cgaaaaaatc ttcagtcctc
     4621 cccactctgc aggccttgca tacaactgta tgggtgggga gtggggggtt aaaccgtaca
     4681 attatccagc ctcgtatagt tgatcgagtg tgtcgcaatt tcgagcacct caacatgatc
     4741 caaagcatcg ctttttatca catatccctc tccaaggaca taattaagca tttaactttc
     4801 aaatatattt caagccactt attcctttta aaccccatta acttcattac tattgatggc
     4861 tcaactccca agatcagcat attcattgca aatgcgtgtt taattgcatc cggcgtgact
     4921 tctactggaa aaatcacgcc atccaatctg gcatgtttaa tagcattatc tatccatgat
     4981 gtcgcaatga aattgtccac atcccatatc ctcctaggtt tggtggaatc gccgcaagta
     5041 ttacccgcta ttttcaaagt agcaatcaat aacttaactt gagctatata atatggggaa
     5101 agtaaaggaa ttttccggcg gggagacaat ctattatcgt tatctaacga caatgattca
     5161 gtttcagaac ttaatacttt aacatatggg cgaggagatt taaaaacaaa agacttccgt
     5221 tctaaatgaa tcacttcagt taatgaagca ccagtattcc ataaagtaga aacaaaagtt
     5281 cgtacactca actcaggcga ataatgcaaa acaatgttaa tctctgtggg taaaatatat
     5341 ggaggaaaac ccaattcaag ggcctgttta cgaaacccct cccccagata caaccccttc
     5401 ggattaaaag cccctgaagt aaggaaaata ctttcaagtt cttttttatt taatggctta
     5461 ttatccatca tatcttatcc atcatatcga cacaaacacc ggccaccaag gccggtgttt
     5521 ggtaaataat taaaactgat atactaaacc aagtgcgaca atgtcatcag tagagatgcc
     5581 agcagcactg gtgaaacgat tgtcatccac caaattgatt ttataatcaa cataagcaga
     5641 catgtttttg ttgaagtagt aggtcgcggc tacatcaaca tatttcagca gatcctgatc
     5701 gccgaaacct tcgatgtctt tacctttgga ctgcaggtat gccacggacg gacgcagacc
     5761 gaagtcgaac tggtactgtg caaccacttc aaagttttgt gctttattag cataaccact
     5821 gacggaatca ccactgaaac gagtcgcatt ataagtttgg gtatactccg cagccaagta
     5881 aatattgttc gcgtcatatt tcaagccacc agtgtaagtt tctgcgtatt tacctcgacc
     5941 aaccagacct gcagcattct ggtcatcagt tcgtttagaa tgagagtatg caaaacctgc
     6001 gctgatgcct tcccaaatat cataagtgat cgctgttccg aagccatcac cgttctgttt
     6061 gttccagccg cgaccattgt tagtctggcc ttcaccactt acactaccgt ttttaccctg
     6121 gtactgcagc gcaaagttca ggccgtcaac cagacc
//
