LOCUS       NZ_CP096245            10060 bp    DNA     circular CON 21-FEB-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD01665 plasmid
            pDD01665-4, complete sequence.
ACCESSION   NZ_CP096245
VERSION     NZ_CP096245.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27753784
            Assembly: GCF_027594945.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Jiang,J., Xu,X. and Wang,M.
  TITLE     The complete genome sequences of 4 K1-ST23 CR-hvKp in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Jiang,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2022) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/21/2024 04:54:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,625
            CDSs (total)                      :: 5,502
            Genes (coding)                    :: 5,346
            CDSs (with protein)               :: 5,346
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 72 of 156
            Pseudo Genes (incomplete)         :: 84 of 156
            Pseudo Genes (internal stop)      :: 36 of 156
            Pseudo Genes (multiple problems)  :: 34 of 156
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096245.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD01665"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD01665-4"
                     /geo_loc_name="China"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017"
     gene            complement(join(8631..10060,1..535))
                     /locus_tag="M0535_RS28035"
                     /old_locus_tag="M0535_27995"
     CDS             complement(join(8631..10060,1..535))
                     /locus_tag="M0535_RS28035"
                     /old_locus_tag="M0535_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(2019..2168)
                     /locus_tag="M0535_RS28040"
                     /old_locus_tag="M0535_28000"
     CDS             complement(2019..2168)
                     /locus_tag="M0535_RS28040"
                     /old_locus_tag="M0535_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(2253..2510)
                     /locus_tag="M0535_RS28045"
                     /old_locus_tag="M0535_28005"
     CDS             complement(2253..2510)
                     /locus_tag="M0535_RS28045"
                     /old_locus_tag="M0535_28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(2520..4205)
                     /locus_tag="M0535_RS28050"
                     /old_locus_tag="M0535_28010"
     CDS             complement(2520..4205)
                     /locus_tag="M0535_RS28050"
                     /old_locus_tag="M0535_28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4532..4777
                     /locus_tag="M0535_RS28055"
                     /old_locus_tag="M0535_28015"
     CDS             4532..4777
                     /locus_tag="M0535_RS28055"
                     /old_locus_tag="M0535_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            5051..5422
                     /gene="tolA"
                     /locus_tag="M0535_RS28060"
                     /old_locus_tag="M0535_28020"
     CDS             5051..5422
                     /gene="tolA"
                     /locus_tag="M0535_RS28060"
                     /old_locus_tag="M0535_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            5419..5784
                     /locus_tag="M0535_RS28065"
                     /old_locus_tag="M0535_28025"
     CDS             5419..5784
                     /locus_tag="M0535_RS28065"
                     /old_locus_tag="M0535_28025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            5959..6237
                     /locus_tag="M0535_RS28070"
                     /old_locus_tag="M0535_28030"
     CDS             5959..6237
                     /locus_tag="M0535_RS28070"
                     /old_locus_tag="M0535_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            6225..6539
                     /locus_tag="M0535_RS28075"
                     /old_locus_tag="M0535_28035"
     CDS             6225..6539
                     /locus_tag="M0535_RS28075"
                     /old_locus_tag="M0535_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6703..7131
                     /locus_tag="M0535_RS28080"
                     /old_locus_tag="M0535_28040"
     CDS             6703..7131
                     /locus_tag="M0535_RS28080"
                     /old_locus_tag="M0535_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            7157..7336
                     /locus_tag="M0535_RS28085"
                     /old_locus_tag="M0535_28045"
     CDS             7157..7336
                     /locus_tag="M0535_RS28085"
                     /old_locus_tag="M0535_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7363..7893)
                     /locus_tag="M0535_RS28090"
                     /old_locus_tag="M0535_28050"
     CDS             complement(7363..7893)
                     /locus_tag="M0535_RS28090"
                     /old_locus_tag="M0535_28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7900..8631)
                     /gene="mobC"
                     /locus_tag="M0535_RS28095"
                     /old_locus_tag="M0535_28055"
     CDS             complement(7900..8631)
                     /gene="mobC"
                     /locus_tag="M0535_RS28095"
                     /old_locus_tag="M0535_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..9205
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1338..1916
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7904..8631)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 catcaagcag caggaacgcc agcagccgcg ccagaaggac gccggcgaca agccctccgg
       61 cacaccaggc tatcccgaac aggttagccg gctgcagcag gtaataatct gcgttcgcat
      121 agaggtccgg gcgcaggacg gtttctcccc agctctcgat aaacacggcg acaaccagcc
      181 cccccagaag ggccagaccg gcagcgccgg tcagccagta cgcgtagttc cagacatgtc
      241 gccgtgccct gaccatccgc ccgggtgaat ccagcgaggc cagcaccgaa aatggcacag
      301 tccggttatt atcgcgctta tagcgatcgg catactcccg ccgcgcctta tggctcagcc
      361 ccacaaaacg cattcggccg gcccagagtt cagccagacc gaaaaacagg ccgataacca
      421 gaaaaaaacc ggccagactg taatactccc ggtacagcca gctgacccac gggtaaatct
      481 gccgggccag ctccgggata tgccactgcc aggcaagcga atccgtatta aacatgcaat
      541 ctctctcctt cagacgacgg gtttaccggc gtgatgcgca gcccccgctg cacaagccgc
      601 ggacatgccc caaggcatgg cggcggcggt cacgtccgtg gtgattgcgt tcagctttca
      661 ccgcggaggc cagcctcttt ccgccccatg cggaaaaggc gcagccccgg aggaacaacg
      721 agcacgcccc agcgtgtgag aggaaaaggc ggcaccccag tgcggtcttt tccggtgttc
      781 cggcccccgt cagagttcgc catagcgagc ggctgaccac ccgcaaaatc aggagcagct
      841 ttgctgcttc gtcaggtgcg tccagcaccg tggatttttg tgggtggtca ggcgtgagcc
      901 tgacgggggc cgggtgaatt tctggcgtgg ccggcgtcca gccggacaga gccagaaaga
      961 ggggaaaaga ccgtgcgtcg gtggcgtcca gccagcgtaa gcaaggtctt ttcccctgaa
     1021 gtcagccacg ccccagcggg gatgacttgc atttctccgc aaggagaaaa aagcaacaga
     1081 gataggttaa cgtaaaatgt tcagcgaaaa accgacatgg ttcacctatc ctgataattg
     1141 atcgtcaggg aatagaaaga cttaatcagg ggacaatatc ccacatcagc gcgtccggtg
     1201 accgtcttgc aggtgccggg gaaattgcgc ctcctcgctc actcggtcgc tacgctccgg
     1261 gcgtgagact gcggcgggcg ctgcggacac atacaaagtt acccacagat tccgtggata
     1321 agcaggggac taacatgtga ggcaaaacgg cagggccgcg ccggtggcgt ttttccatag
     1381 gctccgccct cctgccagag ttcacataaa cagacgcttt ttcggtgcat ctgtgggagc
     1441 cgtgaggctc aaccatgaat ctgacagtac gggcgaaacc cgacaggact taaagatccc
     1501 caccgtttcc ggtaggtcgc tccctcttgc gctctcctgt tccgaccctg ccgtttaccg
     1561 gatacctgtc cgccgttctc ccttgcggga agtgtggcgc tttctcatag ctcacacact
     1621 ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa gaactccccg
     1681 ttcagcccga ctgctgcgcc ttatccggta actgttcact tgagtccaac ccgggaaagc
     1741 acgcaaaacg ccactggcag cagccattgg taactgggag ttcgcagagg atttgtttag
     1801 ctaaacacgc ggttgctctt gaagtgtgcg ccaaagtccg gctacactgg aaggacagat
     1861 ttggttgctg tgctctgcga aagccagtta ccacggttaa gcagttcccc aactgactta
     1921 accttcgatc aaaccacctc cccaggtggt tttttcgttt acagggcaaa agattacgcg
     1981 cagaaaaaaa ggatctcaag aagatccttt gatcttttct actgaaccgc gatccccgtc
     2041 agttcagagg aggacgatgg tgccaccgtt ccgccctgaa catcccggat ataatttgcc
     2101 tgacatgcgg ccagaagaaa gccagcaaca atcaatataa aaagaaaaat agtctttacc
     2161 tttttcatat actttccctg tatttaaaga ctccggcatt catccctgaa caccggaagt
     2221 cagtatttca aatcatcctt gataattttt tgttaccagt taccgtcccg ataatcaaaa
     2281 gctatataac aattatccac atcaggtttt atttgatttt taaaaaatgg ctgtagtaaa
     2341 ggaacccatt ctttcttcaa atcaaactca ccattattaa tattatcctt taaaggcatt
     2401 cccaagcttt caatgacaga accatcgtca ccaaagtctt tagaatactc ttccccttta
     2461 aactcttcag ttttcttatc aaaccatgtc aaacgtaatt taagccccat atttacctct
     2521 caaagatatt ttttaatgtt ccgttttgga tctggccctt taacctgctt acccgttttg
     2581 ggatcaaatg cgcctaggtg ttcgccatca ctggcacgat acccttcaag ctcaccgtgc
     2641 tgggaatccc attcataaat tttacgtttt ttatcgccat accatcgggc ccgcttacca
     2701 ccaccacctt gtttaggtgt tttaggatca cctcgcttta actctccaag tccctttatg
     2761 tcttctgttt taggtactgg atgataatca tggccgtagt ctttagtccc cttgcgaggc
     2821 ttgttctttt cctcatttaa cttattttct gcatccttcg cttttttctc cttttgtttc
     2881 cggctctcca tcgccccggt aagtgcagcc ttatctctgg ataaggattc attcattgcg
     2941 tttaaagatg cctgagccgc ttttaatttc tcattctgat tctgaatatc cttatcaaga
     3001 tagcgaacct gatcctgcca cccggagcca tattcatgag gcgggttata agtcacaaaa
     3061 tcatctttat ttgcctctgc ctcttttacc tttttatcgg cttcctgaac agcagccctt
     3121 gcaccgggga gcgccttctc tgtcgatgca atgcggctgt ttaaggtcgc aatattttta
     3181 tcttcggcat ccagctcagc tttcgcttta tcatagtctc ttttcagccc ctcttccgga
     3241 tgagcggcgt cccatgcctg ctggcgacgc ttttcttcct cctgacgctg ttttacctgt
     3301 gccggggtaa gaacatctgt caccgacaca taaaccggct tctgtccggt ctctttcggg
     3361 aaacgaataa cggcctcacg ggagttacca ccggctgtaa aaccagccgg acgtgaatca
     3421 cctttttcag caacaatgcc ttttggcggg gctttcgctg tcggaacacc tttcggtacg
     3481 ctcacctgca gagacgggag acccggaatc gacacactga atacccccgg acgttttgtc
     3541 ggtttcgcat ccaccacagg gacactcatt ggccgacctg cgacaaccgc aatatgctgc
     3601 cgctcatcct tcaccacatc cacaacccgt tgtctgacac gaaccgtcgc ctggtccagt
     3661 ggtaaagtac ttgccggagt ctccgtcacc gcatcggccg gtaatgacgt cataattttt
     3721 gacatcattt tcggatcatc ttttgcaatc tcagaaggca gcacaccgta gatcgcaatc
     3781 ccccacagac caaacttaaa cggccccttc agggcagcca gcacatcagc aacggcggcc
     3841 gacaacgcat cgccggaaac tgataaagcc agtccttcag cccccggagt agccagggca
     3901 ggcagaccga aggccatcgc agtggccgaa gactgtccgc caccagagct accgctatga
     3961 ttgccgctgt taccaccatt accaccgggt ttactcccgc cgttaccaaa ctcccccgga
     4021 ttatagttat gaatatctcc attcggcgta tgcgtcgtac cccagcccgg cccgctgttt
     4081 ggatccgtac caccacctga tgaagggcca cctttaccag acgtaccgtt cacattccca
     4141 ctggcctgac caccattatg tcccagacct gaattacccg gacctcgacc gtctccacca
     4201 ctcataccgt tttcctcttt gacatttaaa acaataaatt aaagccaaca tactgtacat
     4261 atacacagta taattattac cttactcctc ccgttttcag gtgtcaatat atgagattag
     4321 ttctcatctt caggcacaaa aaagcgcccg gagccacctg taagcagatc gcgtcttttc
     4381 ccgaaaattt ctcatggcaa caaattttat tatttaagac attcatcaca cttacatttc
     4441 agatggtata gacacatctg aaagctaact ggcatgagga gaggacctgc ggttaaactg
     4501 ttcgggaaac ctgagacgcc cggaggccac tatgacgcac ctgaaactga ctcccgctga
     4561 agaaaagcgc ctggcggacg cctttgtcgc tcaggcactg gccatgggcc tgaaagggaa
     4621 caaaacgctg ccaaaggcgg taaaacaaca ggtgcgggaa agggtgcttc gtgccattca
     4681 ggacgaacgt gaagccaggc gggcgcagga aatgcgtcgg gagaaatacg aggctacgga
     4741 aaccaccttc acctggcagc cacagcgccg ccggtaaggg gtatttacca taacgtcgca
     4801 ttgcccgcac cgctcagacg gtctgagggc gtttccggcg gctacgggat aaaacaggca
     4861 acaaaccacc cggaaaaccg ccggaaacgc gcccggtaaa ttgtaacccg ctgcatgcct
     4921 atgcacgcaa ctgcatggcg ggccgtttgg tgtaaaaacg atacgataag ccgtgtttat
     4981 gtaaatgaac gtggccgtaa agagtgacaa atatacatgt aacatttttt actgacaggt
     5041 catcaacccg atgcgtttaa taccctttat cctgctggca ttgtgcagcc ccttactcct
     5101 gacggcctgt aagcattcca ccacccagcc gccgaaaatt tatactctgg ataagtcatc
     5161 taaaatcagt agctacgctg cggccatcaa gaccgctgtt gaaatgcaga tgtttgaacc
     5221 agaggcctat gcgggaaaaa tgtgcaccgt gcggatatcc atacaacgtg atggcactct
     5281 gaacagcgcc attgctgaag gaggagatcc gaaactctgt aaagcagcaa tatcagccgt
     5341 tacccacgcc aaaattccgc cggcaccgga tgaaaatacc tggcaaatat tcaaaaacgc
     5401 ccctctggat ttcagaccat gaaagctttt actctgacag tgctggcggc catgttcctc
     5461 actccggtac atgcgatacc actaaaaccc gtaaccacac tgcagccaac ctacccggta
     5521 caggccgcag caaaacgcat cgaaggtcag gtgaccgcca cgtttgatat tctggacgat
     5581 ggacgagtgg acaacgtgtc gcttaccggc tgtcaccttt tttttcggga aacgctgacg
     5641 gcaatgaggc actggctata tcccgcaggg cacccggcaa tgcgacaggt tataaatttt
     5701 gaattcagtc tgaaaaatga cggcttacag gatccgcgac cagtcaccac ccaaaagctc
     5761 aatgatgatg gttgccgcca gtaacaggtc ctttaacccg caggctgcaa tagtcctgga
     5821 tgatgagtga gcctgtagca gccctgatta gtggaagcga agctgaaaaa tgggcagcaa
     5881 aagccgtatt tctggcaaat agcgccgcca cggattaaag tagtcatgtg taatacatat
     5941 gactacaaag gggcttttat gtcatcagca acatccatca ggctcgatga agaacttaag
     6001 gaccggctga aaacgctggc cgatgaccgg caccgctcag cgcacgcgct aatgctggag
     6061 gcaatcaccg aatacatcga ccgggaagag aagcgcagcc agtacctgcg ggacggacag
     6121 gccgcatggc agcactatca ggaaacgggt ctgcacctta ctgcggaaga agcggaagcc
     6181 tggataagta cctggggaac tgagaacgag caggatgctc cgccatgcca caggtaacga
     6241 tttccgcgct ggcacagcgc gatttacaac gccttcagga ctttctcaaa accaaaaatc
     6301 ggctggcagc cagaaaagga ggtgaggtga tcgtccgggc tatccagcaa ctgaaaacat
     6361 tgccagacat tggccgcccg gtgccgtttc tgccgctgga atataaggaa ctggtgatcg
     6421 ggtttggcga cagtggctat gtgatgctct accgccacga ccgggaaatg gaccagattg
     6481 tgatcgtcac cgtcaggcat cagaaagaat ccgggtatcc gggggcagac agcctctaat
     6541 aaccgatgac gggggcgcaa ttcattgcgt ggccgtcgcc ggtttgacaa gcgcagcgcg
     6601 tcagaaaacc acatcctgaa cggaaaaacg ggtaaaattc agcgatacat ttaccgtgag
     6661 aaattacaca cctttcacac ccgataaaat caggggtata cagtggcgaa aacattgctc
     6721 aacctttcac aggcagcaca agccgcgggc attaccagaa gaacgctata caatcatgta
     6781 aaacagggaa aggttaccgt gtcacgtgac gggaaaaaca accctgtcgt ggatgtgtca
     6841 gaactgatcc gtgtatacgg aaatgtgaat atccctgaga aacaaattcc cggcatttca
     6901 caccgtgaaa atacacaaaa aaatttccca caggaacagc ttctggcaat gcaaaaggaa
     6961 ctggccgatc tccggcaggc tgtcaccctg atgctggaag ataaaacatc ccgggaagaa
     7021 gaacgccgcc agcacgatga tgagcgccgg aaactccagg ccgaagtgga cagattgaca
     7081 acagaactga cacagaagaa aaaacgcttc tggtccggct ggtttagctg agaacttacc
     7141 gaagggaatg gagaacatga cgaagcagga aaaaactgcc ctcaatatgg ctaaattcat
     7201 taaggatcag tcgctgctgt tgctggaaaa gctgaacgag ctggacatgg ataccccggc
     7261 cgacatgtgc gaacggcttc acgaggatgc ggaaacactc tggcaggtga tgcgggataa
     7321 gctcggggaa gaataacagc accggccatg ccggtgctgg cgttattttt tgccgaacgg
     7381 ccaccaccgg cgggcgggtt tgtcctccgg cagttcaggc accggcacgt taaaatagcg
     7441 gctgttatat tcccgcatca gctccgcttt cagcttctcg ttcaccgcgc tttccccgtg
     7501 ccgctcgctg accacctcaa tcatcgcgtt aatctgctcc ggtgagcact tcccggcatc
     7561 aaccacctgc acaccgttct cgatggcgaa ccgctccacc tttcgataga ggtaatcaca
     7621 cagcttcatt cctgtgggtt tgtcccgttc ccattgcgga ttttcaccgg tgaaaaagta
     7681 cgtggctttt tcgtcgctgt tgagccgctc gtcatacatc ccgatggatt tcatcatcgc
     7741 cagatgctgg gcccggtcga caaaacagaa aacatacgcc ccggtctgcc aggcgagcac
     7801 caccatgtct tccagatgct cgctggttgg cataatgcag cgctcgccgt ctttgtcttc
     7861 cacgtcctca ataaatacca tgtacagcgc cataatccct cactccgttg tcataaattt
     7921 aaagcacgcg aacagcctct cccggtccag ctccaccgtt ttgcgggcct cgccggtctg
     7981 tggattcacg atttcatcca ccgccagaat cgcccgctgt acccggacaa gcgtcggccg
     8041 gtcagggcag atgtaccaga tttcatcgat gccgttcgcc cggcggttga acagataccc
     8101 ggacaccact tcctgatacc gcctgcggct cttgacggtg cgctctacct caaatgccac
     8161 cgttctgccg tccagctccg ccagcgcgtc cggcactttc atccctttca gggccatacg
     8221 gtgcaggtac cgccagccgg tggccccccg gccctccatg gagaggcgca gggactgcac
     8281 ggccagccgg tgcggcagct gcgccgccga cacccttgac ggctgaaacg gaataacgtc
     8341 cgtcagcggc tcgtcctcgt cgaacgagaa cgccaccccg tgcggcgtca gcccccacac
     8401 gctgacctga cggccgtcaa ccgggtattt cgcccggacc agcagccctt tcgccaccag
     8461 actgtccagg aagcggtaaa ccggctgtac cgccgcaatc cccagccgtc gcgcaaccag
     8521 ctccgccgtg gaacacagct cttcccgcag ccagcgcagg gtcagccgtg ttttctccgc
     8581 gttccgcgcc tgccgcttcg cgtggctgac gttgtactgc tccagtgtca tcagtaaagc
     8641 cccgcaaaat catcatccgc ttttggtccg ccgccgtcgg ccggaggcag gtactccgcc
     8701 agcgggtccg ttttcgccag caccgggaag gttttcagcg aaatttcccg gcgctcaacc
     8761 cgcagcgggc tgctgaacgc cagccgcgcc acgcccagcc cggtcatcac cgcgaacccg
     8821 tcaggcaggt gttgcagggt gttcacgtca aacagcggcc gcgcctcctt ctggatgaag
     8881 ctgtcgccgc tgatgtgctc cacgttcccc gcctcccggc tggcccggcg gcgctccacg
     8941 tccacccgga tctccccggt ctggcccgcc gcccactgcg cggactcctg gctggccgcg
     9001 cgatagaacc agcggatcgg cgtgttatcc agtaccgtgg ttttcacaaa atcagcgggc
     9061 agatcctgcc cgcactgacc gaagtcgccc agcgactggt gcgcgagcag caggttgcag
     9121 tcccggctgc gcagcgtccc cagcgcgttc agcacgtact tcgacagcag atatttaatc
     9181 tcatcgagca tgatgctcgc gtgcggccac ctccggaact catcccgcgc aatgatgatt
     9241 tgcgcgcagc gggcaaacag catcttctgc acccggatca ccgcctcatc gtccatcgag
     9301 ccgatgacgt acaggcagcc gccgccgttg attatccccg ccacatcgat accgctgtcc
     9361 gtctgcagga cgctcaggtc ggccacgttc tccagcttgg tgatcagccc cttcacgtcc
     9421 tttttcaggg cctccggcaa cgcgtacgcc gccgccagca cctgctgaat gtttgccccc
     9481 tgcgggaact gctccgcaat cagcttcgcc gctttctgct cgctgatgcg gtagtggtcc
     9541 gccacgtcgc cggtctcact caggttaaac ccggcaacca gcagattctt cagggcgtac
     9601 tggtcgagat cgcggaacag attcagctgc gccggtttcc ccggccgcag atccagcagg
     9661 gtgaagtccg ggcagtgcgc ccggaagacg tgcggcgccc acgcgtcccc tttggggtcg
     9721 aagtacacca cagcgtcccc gaaatcgcgt acgcagcgaa tgagcgcgtt ggtggccatc
     9781 gcgctcttgc cgctcccggg caggccgagg atctgaatat tggtcttgcg ccaggtatca
     9841 cgagggacca gcaccggctg gccctgctca tccttaccca gaaacaccgc ctgctgtttc
     9901 cgggcctgct ccgtggaaaa atcggccagc gcgttaaccg gtaccggctc cccgaaatgc
     9961 aggtggcgga cgtctgtcat ttctccggtc cgctggctgc tgcgccggct ctcctgattc
    10021 agtttcgcgt tcaccgcctc gtcagcggcc gcaaaatgac
//
