LOCUS       NZ_CP096226             2189 bp    DNA     circular CON 14-MAY-2024
DEFINITION  Enterococcus faecium strain UCH5 plasmid pUCH5-7, complete sequence.
ACCESSION   NZ_CP096226
VERSION     NZ_CP096226.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27728132
            Assembly: GCF_023204975.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Enroth,T.J., Kirsch,J.M., McCollister,B.D., Johnson,C.N. and
            Duerkop,B.A.
  TITLE     Enterococcus faecium UCH5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Enroth,T.J., Kirsch,J.M., McCollister,B.D., Johnson,C.N. and
            Duerkop,B.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2022) Immunology and Microbiology, University of
            Colorado Anschutz Medical Campus, 12800 East 19th Ave, Aurora, CO
            80045, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 24-MAR-2022
            Assembly Method                   :: Unicycler v. 0.5.0
            Assembly Name                     :: UCH5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 247.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023204975.1-RS_2024_05_14
            Annotation Date                   :: 05/14/2024 04:32:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,809
            CDSs (with protein)               :: 2,809
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 52 of 136
            Pseudo Genes (incomplete)         :: 75 of 136
            Pseudo Genes (internal stop)      :: 34 of 136
            Pseudo Genes (multiple problems)  :: 21 of 136
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096226.1.
            Bacteria and source DNA available from Duerkop Lab, University of
            Colorado Medical School, Aurora, CO.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="UCH5"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pUCH5-7"
                     /geo_loc_name="USA"
                     /lat_lon="39.742630 N 104.842026 W"
                     /collection_date="2019"
                     /collected_by="Bruce McCollister"
     gene            553..1518
                     /locus_tag="M0F39_RS15050"
                     /old_locus_tag="M0F39_15050"
     CDS             553..1518
                     /locus_tag="M0F39_RS15050"
                     /old_locus_tag="M0F39_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002305972.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVGK
                     LKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKYQE
                     GQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQLRRG
                     KATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYKYRNLL
                     KKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            1977..>2159
                     /locus_tag="M0F39_RS15055"
                     /old_locus_tag="M0F39_15055"
                     /pseudo=""
     CDS             1977..>2159
                     /locus_tag="M0F39_RS15055"
                     /old_locus_tag="M0F39_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          632..1515
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.588"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 ttacaagtgg gaaataaaaa gctgaaagaa gaaaattgca tttcaaacta cattttctac
       61 aatagctcta aaatggcata ttttgcgttc tgagcggttt tagcgtttat ttcgtttagt
      121 tatcggcaca atcgttaaaa cacgttagag gagcgaaaat ggagcctgta aggcgactgg
      181 tagcggaacg atcgtgttag attgtgaaag ccgatagctg aatgaaataa acagcgggtg
      241 cgccctttcc atttcggcag gaggaggctc aaggggttcg gggaatgaaa ttccctgagg
      301 tctttttatt tttttaggag gagtgctata atcaaagaaa acgagaaaaa agagcaaaat
      361 aaaagccgag cagtgcgcta acactctcga ctatttacgg aaactacttc acctagctcc
      421 gtaactcagg ttcattatac gaatattttt aggtttcttc aagctagaga tttttggata
      481 tgaattttta gggttacgtg aactctggaa attctttcca aaagtctctt tttctttgag
      541 gaggaacaaa atttgagtaa acgtggcacg atcataaatg agtgggaaaa tttaacagcc
      601 ctatctaaca ggcggttaag tgtgcaaact ttgaccaccc cccttaaaat acagtggtca
      661 attgaccgaa ttaccattgt tggaaaactc aaagagaata tttattatca cacgcaaaat
      721 gatgtattga ttttagactt tgaacagtta atgcgactga atgaaaataa tggttatgtt
      781 aaatctgttg gtctaaatgg ttggcaaatt gtggataagc atgatgagaa tattgcttac
      841 attgaaattt tgaaatatca agaagggcaa gggcgaattg actttaaccc aaataaaatt
      901 agccaatttt tggcgggttc gatgaagaac tttatccatg atttattttt agaaccacat
      961 ttctcacgtg ctgatattgc ttgcgacatt atcgatgtgc ctgatgattt tattacgcaa
     1021 tatcgtgtgg ttgaccctgt ttcattcaaa ccaatctatg ggcggtctgg gaagctggaa
     1081 acggcatatt ggggtagtcg tgccagtgag cgacaaataa ggatgtacaa caaaaaattg
     1141 gaacaagaaa ccaaacggaa aattgtacca ccggaaataa aaacttggtg gcgactggaa
     1201 ttgcagttac ggcgtggaaa agctactgat tggtatgcga tggttcatga aagtttagac
     1261 agtttcgcaa gtcctcattt tttgccgact gatacaaagc atactgatag aattatgatt
     1321 gcaggattga tttcagatca aaatttatgg gctggcttgg ctcgtcatac aaaatataaa
     1381 tatcgaaatt tgttgaaaaa agaaagccaa agtgatgagt tagttaatca tttacgagag
     1441 acattttcgg aatcggcaga tgttttgaaa aaagaattgg atacgtggct tttggggttg
     1501 gacgtgacgg aagaatgagt ggatgaattt ttgtccactt ttttattttc agcacaagtc
     1561 ggcatggtgg gtcgtgcgtt tgcagcaaga gcgaaaaaac gctaacgaat ccgcaaatgc
     1621 cgacttggat aaaacagttg cgcaaagaga cttggctttt tataataaat ttagggatat
     1681 cccagtgtca ctctttgggg taattcgggg gaaggagtta cccctcactc tggaaaatta
     1741 gagcgaagca gacgagatta tcccaatgaa tatgacggct tactcggagt gataccgagt
     1801 aaaaaagtca gataagtgag agtaaattta cgtcaatgtt taagataagc gattattttg
     1861 cgtgaagcaa aataaagcgt aaaaggagtt gagagcgtga cacgctttcg gctccttttt
     1921 tcgttgtcac ttgtgacagc gaaaaccctc ggagagcgtt tggaattgtg acagccatgt
     1981 cacaattagg aaaatgttac tctttgtcaa agagtaacag aaaggtttac actcttttta
     2041 ttagcgacag cgcgaaggag gggttcacct tagggtttag tatttcgttc aaaaagggaa
     2101 caaagaatac aagcatttct cataataatc gtgagttaac tgatagccaa aaaatcaatg
     2161 gtttgtctgg tagaaaataa ttagcagat
//
