LOCUS       NZ_CP096225             3007 bp    DNA     circular CON 14-MAY-2024
DEFINITION  Enterococcus faecium strain UCH5 plasmid pUCH5-6, complete sequence.
ACCESSION   NZ_CP096225
VERSION     NZ_CP096225.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27728132
            Assembly: GCF_023204975.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Enroth,T.J., Kirsch,J.M., McCollister,B.D., Johnson,C.N. and
            Duerkop,B.A.
  TITLE     Enterococcus faecium UCH5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Enroth,T.J., Kirsch,J.M., McCollister,B.D., Johnson,C.N. and
            Duerkop,B.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2022) Immunology and Microbiology, University of
            Colorado Anschutz Medical Campus, 12800 East 19th Ave, Aurora, CO
            80045, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 24-MAR-2022
            Assembly Method                   :: Unicycler v. 0.5.0
            Assembly Name                     :: UCH5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 247.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023204975.1-RS_2024_05_14
            Annotation Date                   :: 05/14/2024 04:32:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,809
            CDSs (with protein)               :: 2,809
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 52 of 136
            Pseudo Genes (incomplete)         :: 75 of 136
            Pseudo Genes (internal stop)      :: 34 of 136
            Pseudo Genes (multiple problems)  :: 21 of 136
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096225.1.
            Bacteria and source DNA available from Duerkop Lab, University of
            Colorado Medical School, Aurora, CO.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="UCH5"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pUCH5-6"
                     /geo_loc_name="USA"
                     /lat_lon="39.742630 N 104.842026 W"
                     /collection_date="2019"
                     /collected_by="Bruce McCollister"
     gene            266..352
                     /locus_tag="M0F39_RS15030"
                     /old_locus_tag="M0F39_15030"
     CDS             266..352
                     /locus_tag="M0F39_RS15030"
                     /old_locus_tag="M0F39_15030"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_125669474.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            1052..1336
                     /locus_tag="M0F39_RS15035"
                     /old_locus_tag="M0F39_15035"
     CDS             1052..1336
                     /locus_tag="M0F39_RS15035"
                     /old_locus_tag="M0F39_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     gene            1339..1755
                     /locus_tag="M0F39_RS15040"
                     /old_locus_tag="M0F39_15040"
     CDS             1339..1755
                     /locus_tag="M0F39_RS15040"
                     /old_locus_tag="M0F39_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001232859.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(1892..2611)
                     /locus_tag="M0F39_RS15045"
                     /old_locus_tag="M0F39_15045"
     CDS             complement(1892..2611)
                     /locus_tag="M0F39_RS15045"
                     /old_locus_tag="M0F39_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002311815.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKK
                     VRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINK
                     TKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVR
                     IEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFS
                     SLTSDLPKISLRNWLEE"
                     /name="replication initiation protein"
     Typing          complement(1893..2611)
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttacttgca gcagtcgcct ttaggctcct tcgccaaaaa taattcatta gggactcctt
       61 tgtggcgtcc tgagagctcg tctaagccgt tctaagacaa gattcgataa aaactatgtc
      121 cttcgttcta aagcgcctta caggcggtca gggcaagccc taacaacccc aaggcaataa
      181 aaaacttagg cttatttttg cttttttatt gtataattga ttgtcgccta tccgacagtc
      241 ttgcgactgg aaggaggtgt gtttcgtgtt cactaacttt atttgctccc ttcttgttgg
      301 gcttttaagc gctttgttca gttactggtt gaataaccga aacaagaagt aaaacttaat
      361 taaggcgact cagcccacaa aaaaatcccc agagaatccg acctctctgg ggatttcgtg
      421 tgtttcgtgt tcacttattt gcaatcaaat tataacagaa ataaatattt atttcaaaaa
      481 tatcctacaa aagtctaaaa tccgtaatta tttcttatat gcaacattta caaataaagt
      541 cgctctgaga gctcgtattt ttcgatttaa gacgttttat ttagtttttg gtgtatttca
      601 ttggtccaag ctaaaaaact cttagaagcg attttagagc catttaaacc cacttaatta
      661 tattctccat gaaattcgct ccatgcgtca aaagctgcgt ctgaaattcc gccgtcttcg
      721 ttcactagtt gcattaattt ttccaacgat cgttttcttt cgggtgtcat atttttatat
      781 tccaacaatt tttcgtctgt taaatttggt tatcgattac acgtgcagca ctcccccccg
      841 aattttaaag agcataagga aacgtgtacc cactttctaa aaaaagtggg tagataggct
      901 gtgcctatca cgtttctcag aaaaaaattt gatatcactt tttcgccatc gtgggtagct
      961 tatgtatagc attttggcaa aaaacagcta tacaaatata gtatttgtat ggtatacata
     1021 aattatacat aattgcaaaa ggagattttt tatggacatt ttagttcgga atattgatcc
     1081 aaagtttgtt gcaacaatag acaaacgttg ccaagaactc acgaataaaa caggagaaaa
     1141 atggagtcga aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac
     1201 gacttacaaa aaagaccaat tcgatttatt gctcgataaa tttattgcta tccaaacaga
     1261 aaatactcaa atattaagcg agtatgtcta cacaaataat caaatcattg aaatgcttat
     1321 cggaggaatg atataaccat gagaaaaact tctgtccaaa aacatgttac tttgtaccct
     1381 agtcatgttg acgttttaga ttccatcatc gaaaaaaatc cagatatcaa taactactcg
     1441 gaagccattc gctttttatg tatgagttat gaacccgata ccaacacgac cactaccagc
     1501 aatcaggcaa aactaaatgc tatgggaaaa gaaatttcca tcatgactga aatcatcgca
     1561 gaatttgctg atttatatct aaaagataca ggcgttctta ttggcacaaa ttctaaagta
     1621 tatcaagaag tgaaagaaaa aatcgaagct aaactaaatc catcaaatcg aaatcgacca
     1681 aagaatcatc aaaaagaaac cagtcaaaca gtcacttcta gaaatttcga cccaacaaat
     1741 tttctaaaac gataaaaaac agataggagt tattcctatc tgttttttat ttagaagccc
     1801 catacaatat gctagaatgt actagcaatc ttagtcggtt gtaagggact tgtctacttg
     1861 cttaatgtaa ggggggcgag ttctttttta tttattcttc taaccagtta cgcagagaaa
     1921 tctttggcaa atctgacgtt aggcttgaaa aagtaaattc taatttggcg attttattgc
     1981 ccttttttgc ttttattttt gtgactttaa gatcattgaa gtattttttt agttctcgta
     2041 atgcaggatt taatactttt tgatcaatgt tacccatttg atagtattct gggatatcca
     2101 ttaagctcct aaactcttca atacgaacta cataataacc agtagaacgg tactgcataa
     2161 gcagtcggaa tagagttttt gcatacgtag aacgcaacga cgtgaaagca gataattcaa
     2221 atctactaaa ttcagctgtt aaaccattaa taatatattc taggtccgga ttaacactga
     2281 cttcaacaaa cttttttgtt ttattgattt caaaaccagt aaacaaaaca aagtagcgaa
     2341 tagaatcctc atcttcagta cggtaagtaa gattaagcat atctttatag agcttttcta
     2401 aatcaccgac aaaggctttt gtagcagtca tcttataatc acttaactct tttaattcct
     2461 cgaaagaaaa tctaactttt ttcaaaccac gatctttcat acgagcgcat aatgcaaaaa
     2521 ataaatccat ctgcttagca ttgaaatttt tcagcggtac taaattcaat tcattttgat
     2581 atttgactgt taattccgtt ttttcatcca ttcctttctt ccccccttac aaataaataa
     2641 taacctttaa caaccaactc gtaaataggt tggttgttat tatacaaaag gatattagta
     2701 ctcagataaa aagttgttag tactcagata aaaagttgtt agtactcaga taaaaagttg
     2761 ttagtactca gataaaaagt tgttagtacc cttctgtatc cgttgggaga gtaaggctca
     2821 actgccccct aaaaagggtt ctaaaaaatc tttaaaaaat aaataaaata aacgcgcgcg
     2881 cgcgagcaaa atttcccgcc aaaaaatcac ccacttttta aaataaacag gttcgcgcat
     2941 gatattaaac tgccaatcta gctccctatg gtcgctgtaa gaaggagtga tcgctacgct
     3001 ctcacat
//
