LOCUS       NZ_CP096223             9317 bp    DNA     circular CON 14-MAY-2024
DEFINITION  Enterococcus faecium strain UCH5 plasmid pUCH5-4, complete sequence.
ACCESSION   NZ_CP096223
VERSION     NZ_CP096223.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27728132
            Assembly: GCF_023204975.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Enroth,T.J., Kirsch,J.M., McCollister,B.D., Johnson,C.N. and
            Duerkop,B.A.
  TITLE     Enterococcus faecium UCH5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9317)
  AUTHORS   Enroth,T.J., Kirsch,J.M., McCollister,B.D., Johnson,C.N. and
            Duerkop,B.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2022) Immunology and Microbiology, University of
            Colorado Anschutz Medical Campus, 12800 East 19th Ave, Aurora, CO
            80045, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 24-MAR-2022
            Assembly Method                   :: Unicycler v. 0.5.0
            Assembly Name                     :: UCH5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 247.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023204975.1-RS_2024_05_14
            Annotation Date                   :: 05/14/2024 04:32:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,037
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,809
            CDSs (with protein)               :: 2,809
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 52 of 136
            Pseudo Genes (incomplete)         :: 75 of 136
            Pseudo Genes (internal stop)      :: 34 of 136
            Pseudo Genes (multiple problems)  :: 21 of 136
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096223.1.
            Bacteria and source DNA available from Duerkop Lab, University of
            Colorado Medical School, Aurora, CO.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="UCH5"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pUCH5-4"
                     /geo_loc_name="USA"
                     /lat_lon="39.742630 N 104.842026 W"
                     /collection_date="2019"
                     /collected_by="Bruce McCollister"
     gene            complement(join(8897..9317,1..41))
                     /locus_tag="M0F39_RS14940"
                     /old_locus_tag="M0F39_14940"
     CDS             complement(join(8897..9317,1..41))
                     /locus_tag="M0F39_RS14940"
                     /old_locus_tag="M0F39_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000389746.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            174..548
                     /locus_tag="M0F39_RS14945"
                     /old_locus_tag="M0F39_14945"
     CDS             174..548
                     /locus_tag="M0F39_RS14945"
                     /old_locus_tag="M0F39_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000713874.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            602..2728
                     /locus_tag="M0F39_RS14950"
                     /old_locus_tag="M0F39_14950"
     CDS             602..2728
                     /locus_tag="M0F39_RS14950"
                     /old_locus_tag="M0F39_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002289343.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            2725..3345
                     /locus_tag="M0F39_RS14955"
                     /old_locus_tag="M0F39_14955"
     CDS             2725..3345
                     /locus_tag="M0F39_RS14955"
                     /old_locus_tag="M0F39_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            3459..3596
                     /locus_tag="M0F39_RS14960"
                     /old_locus_tag="M0F39_14960"
     CDS             3459..3596
                     /locus_tag="M0F39_RS14960"
                     /old_locus_tag="M0F39_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            4038..4418
                     /gene="mobC"
                     /locus_tag="M0F39_RS14965"
                     /old_locus_tag="M0F39_14965"
     CDS             4038..4418
                     /gene="mobC"
                     /locus_tag="M0F39_RS14965"
                     /old_locus_tag="M0F39_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            4400..5314
                     /locus_tag="M0F39_RS14970"
                     /old_locus_tag="M0F39_14970"
     CDS             4400..5314
                     /locus_tag="M0F39_RS14970"
                     /old_locus_tag="M0F39_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002305249.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5274..5552
                     /locus_tag="M0F39_RS15255"
     CDS             5274..5552
                     /locus_tag="M0F39_RS15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            5554..6243
                     /locus_tag="M0F39_RS14975"
                     /old_locus_tag="M0F39_14975"
     CDS             5554..6243
                     /locus_tag="M0F39_RS14975"
                     /old_locus_tag="M0F39_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(6364..6450)
                     /locus_tag="M0F39_RS14980"
                     /old_locus_tag="M0F39_14980"
     CDS             complement(6364..6450)
                     /locus_tag="M0F39_RS14980"
                     /old_locus_tag="M0F39_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            7095..7832
                     /locus_tag="M0F39_RS14985"
                     /old_locus_tag="M0F39_14985"
     CDS             7095..7832
                     /locus_tag="M0F39_RS14985"
                     /old_locus_tag="M0F39_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            7860..8399
                     /locus_tag="M0F39_RS14990"
                     /old_locus_tag="M0F39_14990"
     CDS             7860..8399
                     /locus_tag="M0F39_RS14990"
                     /old_locus_tag="M0F39_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            8415..8585
                     /locus_tag="M0F39_RS14995"
                     /old_locus_tag="M0F39_14995"
     CDS             8415..8585
                     /locus_tag="M0F39_RS14995"
                     /old_locus_tag="M0F39_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     BGC             1..5548
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7096..7832
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4401..5311
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcactctta attctcttag cttagatcct aaatcctcca ttttttctcc tatgtaaacg
       61 attatttaca taaaatttta tcataaaaat actaacgcgc caaaaaagtt ctttatttta
      121 taaaacacac taaaataaaa taattctagt aaaaaagata ggagaaataa gttatgaaaa
      181 aatttttatg tctttctgct ttaatgggtg tcttattaac tagtggagga atagttagtg
      241 ctacagaagc tttaaatcta gatgtacccg aagaccatac cgccatttac ggaggaggaa
      301 tggaagctac agaagaagga ttttcttcta gtaaattgag atatgctgct ggcggcggag
      361 atttcaactg tggggttaat ggatttaaag tttatgcaaa ttactaccat gctagagcta
      421 aacatagtgc aacagctaaa aatggacgag gcggtcaagt acgatcagtt cagaaagctg
      481 gagtaagagc ttatgcaaca tgcaatgcca cacttactgg aaatactggt tggtggaatg
      541 tttattaaaa aagagagttt aaactctctt ttttatttac atttgaaagg aaattcaaaa
      601 tatgttaaaa aaaataaacg ctgttttatc actttttgtc tgcattacaa tactattctt
      661 ttctactaat gttataaatg gtataaagac ttctgaagcg ctatataaaa ataccgctga
      721 aattcatatg caatataaag atgatatgga ctttaacagt tatataaacg ctttgtatga
      781 aatatctaca caagaaaata ttaatatttc tcaatatagt tttactgctg ttaaccaact
      841 aactattctt gctacaaacc ctaacgctaa caaacactgg aaattttcta aaaaaaatat
      901 attagcaaga aacgattcta aagtccatta tactaataac aaaaattcta agtaccaatt
      961 aaaacttcca aataattttt taaatataaa tatttatcct ttttctcatg tggaaaatat
     1021 tggattaagt gaaatattat atgtacaagg aaactcaaac aaattaattc ctgtactgaa
     1081 aaaatatgga acacctacta tttccaatat caatcaatca gatacttttc aaattaatac
     1141 taatattgtt cttgttatct gctatcttag tatatttatt gtaattacta caattatatt
     1201 tgctttttca aaaatgaaag aaatcactct aaaaaaaatg ttaggctata actcttttga
     1261 tactataaaa gattctatta aagagttaaa cctcttattt tgttctttga ttataacttt
     1321 cattttatct atttttttga gcatatatta cagtaacgaa aattttttcc tatcatttct
     1381 tctatgtagt atttttcatt ttataattag ttttttattt atcatagtta ctctaagtat
     1441 ctatcattta tctagtataa aaaattatct aaaaaattca cgaccactta agttaaatct
     1501 ttgtatatta aatatctgtc tattcttaag catgatcctt ctgttaatag cttctacaaa
     1561 agtaataaat atccataatg aagccgaaaa taattctctt aaatattggg aaagaactac
     1621 taatttatat aaaacaaata ttactaatca attaaataga aacaacaccg tagaagaaaa
     1681 taactacttg aaaaaagcta gtaaatttgt ttataaaata cagaagaatt ataaaacttt
     1741 tattatcgcg ccatacaatt acgcaactat ccaagaaaat aataaagaat tttttattgg
     1801 acaaaaagtt tatccaaatt ttgaagacta tgttagtcaa cctgctggtg caggaatatg
     1861 tgttgattta aactatctaa aatattacaa tcctatttct tttgaagaat ctactgactt
     1921 aaatttaata aactctgatc cactaactac ttacattcta gtacctaaaa aatataaaga
     1981 atatgcaaaa atgattgaaa aaaattattt agaagacata cagtttagac ttgaagactc
     2041 tcctcctcaa gctccttata aaactcctaa tttgaaaaat ttaaaaataa aactaatatt
     2101 tgttaataat aaccaaaagt attttagttt taatacctta tatggaaagg caaaagataa
     2161 ctatacaata actgatccca tagttagagt tattaatcca gaaataacag aatcattatt
     2221 ttggggaaat atattgacta gtgatggtgg attatttttt gatcagaaac atacttcaaa
     2281 ccaaaacttt tttaatcaaa ttaatccgta tataaaagaa tttaacctag aaaatatcat
     2341 aaatttttct tattctgttt ttcaagaaac tggtgaatta ttatctcaaa aaaaagtaaa
     2401 taaattaaat gcatatctac aatatctttt aatattagga ttatttataa taattaattt
     2461 tcaaatgatt tttgttagtt ttactataaa ttcaaaaaaa atagctttaa aaaaaatatt
     2521 tggatattca ccaataagtc aagttttaga tttatgttta ctccctttta caatagagtt
     2581 atttgctact atgtgttttc aattgatatt ttcaaaaaac acttacttgt attttataat
     2641 ttttatgatc tttttaataa atctaattac ttctattatt atttataaaa aaaatagttt
     2701 taaatatttt aaggagaata ttttatgata ttatcagttg agaatttcac cctgtcaatt
     2761 aataaaaaaa agatcaaaca aaattggaac ttttcaatat cagaaaaaga ttttattata
     2821 gtaacaggaa aaagtggtag tggaaaaact actctgttaa ataatttaag cctaatagat
     2881 aaacaatata ccggtaactt attctatttt gaaaagaaag tttctaaaca tgatatacaa
     2941 cgtctacgaa gaaatcatat tagctattta tttcaaaact atgctcttct tgaaaatcaa
     3001 acagtacaat acaatttcga attagcaaaa aagtttaaca gtaatatttc caatgatcat
     3061 atttataatt tattaacaga tttttctttg ccaaaagata tcttaaaaga aaaaatcttt
     3121 ctcttaagcg gtggagaaca acaaagaata gcattaatta gaaatatgtt aaaaccattt
     3181 gatatattat ttgcagatga acctacagga aatttagata aagaaaactc aacttttata
     3241 atgaactttc tgaaaaattt aacagaaaaa gaaaaaaaag ccgttgtact agtaacacat
     3301 gacttaaatg ttctaaacta tggaactaat attatagaat tataattttt acccgtgatg
     3361 ctagctgatt taaaactagc accacgggta tttattttat attccatctc tcctatccca
     3421 aaatttaaat aatcacttaa taaaaaggag gcacccaagt gattttattg aaaaaagagt
     3481 atgaaaaatt ttatgataga cttatttatt ctttgcaatt gccaaaaaaa ttaaaccgtc
     3541 aattacaaaa gacttatgaa actaaatcaa ctgaagaatg ggagagttat tgctagattt
     3601 ttggtagaag taacaaaaag tggacatatt atcataatga ctcgccgaaa gtctgataaa
     3661 aaattggcta aaaatattgc tgaatgttta ataaaactat cagttttagc taaatagtta
     3721 gtagtttaca caaaaaagca tttatgtttt ttgttgcaaa gcaacaattt catcttcggt
     3781 gaaataagcg aaactttaca aaaagtggct agcatttgct agcttttttt gtattgtgga
     3841 gcgtggcttt tgagggggat tcaaggggga gcttgcgccc ctttgacaag cggttatttg
     3901 caccaaatac ggaggtattt ggctagcaaa tatagagctt gccaaaccct tttagtgagc
     3961 tttactcatt gtaccttttt aggtacaatg gtattgcgaa cgatacgtcc gtaggacgta
     4021 ctgaaagggt gggacttatg agcgaacaaa atcaaaattt ggctagcaat tcttctaaaa
     4081 aatataccta tcgatccgag ccaaaacaaa tcagttttcg agtgagcgaa tccgaatttg
     4141 caaagctgaa gcaatcggct gaagctttgc aaatgagtgt gccggctttc gtgaaagcca
     4201 aggcacaaaa cgcgcgtctg gtgacaccga aagtggcgcc agacattgcc caggcaatcg
     4261 cgcgcgattt agccaaagct ggagggaata tcaatcaaat tgctaagtgg tgcaatacac
     4321 accaacatga cgttgcttct ggtgacgctc aacgtttatc cgaaaaccta aaaatcatgc
     4381 aaaaggagct acaaaagata tggcaacaat taaggtaagc cgaaccactt cttgtagtcg
     4441 tgcaattaat tacgcagaac cacgagcaac cattaaaaca ggaataaact gtgacgtcga
     4501 ttatgccaaa agtgaaatga aacaaattcg catgctgtat ggaaaagacg accgtgtaca
     4561 ggcacactta ttgattcaat cttttcgacc aggagaaatt accgctgaaa aagctaatca
     4621 actaggggca gagtatgcaa aaaaaatagc ccccgatcac caaatagcta tttacacaca
     4681 tacagacaaa gaccatattc ataatcacat tgtcattaac tccgttaatc tggagactgg
     4741 aaaaaaattt caagctcatg gtcaagcatt tctaaacaaa tgttatgacc taaatgacga
     4801 gatttgtctt gcacataact taccaatcac tgagcgagga aagaaaccag aaaaacgaac
     4861 catgtctgaa ataaaattaa aagaaaaaaa tgaacctgtt tggaaagacg aaattcggat
     4921 tgcgatcgat caaacaatga aaaacaaaaa aaccagaacc tatgaccaat tttgtaattc
     4981 cctcaaaaca ttggggattc attgcttcaa tcgcgggaaa aatttcactt acgaattgat
     5041 ccacaaaaag aaaaaagtac gatccaacag actaggaaag gactacgaaa aggagacgat
     5101 tttacatgag ctggacagac gagaacgaac ctataaccaa cagcgaacca acgagctacg
     5161 aagagctcga gctagcattc gcgaaactgc agaagaagta cgaagagaac aacgaactct
     5221 tacgcctgaa caaccggaaa ttagaccggc tcgaagaaac catgaacaac acgttgaaag
     5281 aggaacagac caccaacaag aattatcgct ctaaaacgtt aaaagacata gaaaacgaaa
     5341 taaagaaaca acgtgctttt ctcgttcctt atacgcaaga aaggatacaa actacgattg
     5401 atttacaagt taaaaaactc catcaagagc tattagacca gcgaaataag gaacagaaaa
     5461 aggtgcttat tcaacgatca ttggattttc ttgccccctt atgttgctta gtcctgacac
     5521 tgttcgcatt ttggttagta tggggaggct aaaatggcat tttatttacc ctatctactc
     5581 atttttgtta gtatttcagg aagtatttgg ctcatctata aaatttttca aacaagatat
     5641 tcattaaaag gctctaaaat tcgatttaag cgatttttct ttcttggctg tatattttcc
     5701 ttgataatcg tttcaagcgg cttattcggc gttttagagg gaaataagcg cgtttctaga
     5761 agtattttat taggaaatgt cactcaaaag tatgaatcag cgcgtgataa aaagaaaaaa
     5821 gagcaagcat tagcacaaaa aatggaagag tttaccactt gttatgagga aatgaacgat
     5881 atttttgtga atcaagaaaa acgtttgacg gataaaaaca tggaaaaact cactcgtctt
     5941 tatcaaaact tgccagaaaa acagcaaaaa gaggttcaag acaactatga gcgaaccaaa
     6001 aaagacgtgc aatatgtaaa agatacaaag atagaagaag cctgtagtga tctatttgga
     6061 gacacaaacc cttggtttgc cagtgaagaa gaaaaaaaag aaaaacaaca atctgtcaca
     6121 tatgaacgct atgaaatctt acttcaacaa gcaactaaca tgcaaaatcc gacaaaaaaa
     6181 gaaaccgcct taaactatct aaaaagtgtc aaggaatggt tagatcagca acaacaaaat
     6241 tgaatttgat ttaaaatcat gttaaactga atgtgcaaat gatcaaacat tagatacacg
     6301 aaagccccag agaggtcgga ttctctgggg ctttttttca ggttgatgtc gcctttattg
     6361 attttacttc ttgtttcggt tattcaacca ataactgaat aaagagacac aaactcctac
     6421 aagaaggggg caaatgaaca gttcaaacat tagatacacc tccttccagt cgcaagactg
     6481 tcggataggc gacaagttat tatatcttaa aaaattaaat ttgtgtggtg tttttaagta
     6541 tcacttgttt tagggttgtt agggcttgcc ctgaccgcct gtaaggcgct tctcgactag
     6601 attaagggaa attaatcaag aattgtctta gaacggctta gacaagcttt cagagtctca
     6661 cacctcctct ttgaacaacg gttttagcaa aggagccgga ggcgactgcg cagtataata
     6721 tgagagcata gcgatcacac ctttttagcg accgtaggga gcaagttttc cagtttagaa
     6781 aaattcgcga acctttttgg aggaaaaagt aggagttttt taggcgggaa attcaatgtg
     6841 ctcttaagat atactgttag atatactgtt agatatactg ttaataataa ctgttaggga
     6901 ttagcaacct tatgggagag taagtattga cataggtagt gggggataaa ttgtcacact
     6961 atgggggata aattgtcaca ctatggggga taaattgtca cactatgggg gataaattgt
     7021 cacactatgg gggataaatt gtgttttaca tcctcttatg tctatgataa cataatttat
     7081 tgaggtgatt tttattgagt aacgatatag ttcgatataa taatggatta aattctgtcc
     7141 ctttaagaaa atttacccct atagatatgg atttattttg gggaatatgt tctaaaatga
     7201 aaagaaaagg tacaaaagaa gaaattttta catttaaaca aataaaagaa ttaattgatt
     7261 acaatataaa tgcacgtggc gaccgttttg ctaatgattt aataaaaatg tcaaaaaaaa
     7321 tcattacttt gtcttttatt tatgaagacg aaaaaacata tgaacaatta aatctgtttc
     7381 aaaaatttaa aatcgataaa gtgaatgaaa cattattaat aaaggtttct gaagaatttg
     7441 aatatatact taattcaatc ggaactaatt ttactcgttt cgaattagaa aatatgacaa
     7501 acttaaagag tagctacgtt aaagagttat atcgccaact tatggctcat aaagacagaa
     7561 gatctaaaaa aggtcactgg tttgtaaaaa tagaagattt tagagctcta ttagcaatac
     7621 cagaaagtta tcgcatgaga gatattgata aacaaatttt tgcccaagcg aaaaaagaat
     7681 ttttaaacgt taattcaact caccctgcta tttttaaaac ttttgaaatc aaaaaaatta
     7741 aagcctacaa aggaaataag ataggatctc tgcaaattta ttttgaagaa aatatacctt
     7801 caattacctt agataattgg ttagagaaat aatacctaac aatttaggag gcaattatca
     7861 tgcaacccaa agaaaccttt acaataaagg aattatctga attatttgaa ataagtaggc
     7921 aagcaatctc taaacatgtt gataaattag acccgtcctt attagctaaa aatgagcgtg
     7981 gttataaggt tgtattacgt tctggagtat tgcaacttgc tagcaactta ggtaaccaaa
     8041 ggttactata taggttgcat gagtatgaac aagaaaacga taaaaacgtt tatatatcaa
     8101 gcaaaaacgt gattgtaaac gagttgctag ataagttgcg tgaagaaaat gaatttttac
     8161 gagaacaact ttccctcaaa gagcaaaata attctaatat gcaaaaatta ctcgaccagc
     8221 aacaacagct caccctacag gcaaatcaac agatcgaaaa actgcaagaa cagttacaat
     8281 tagcttatac ggaagaaact ccagaagatg aatcaactac tattctttct gaagaaacag
     8341 aatcgataga aaaacacaca aaaaagaaga agcaaaaatg gtggcaactt tggaaataac
     8401 tggagggaac attcatgaca agaaaaaatc ttctattcgg ttctatttta ctaggaattc
     8461 taggcttcag tccgatgtta ttgtcagtaa tcaataaagg gaataaacat ggtgaattga
     8521 aattgaatcc aaattattac aacagtccta acgaagaatt gtttgtaaaa gagacggata
     8581 aataataaaa aagactgctc ttccgagcag ccagttaaaa aataatgagt gtttattctt
     8641 gaaaaagcac gcgcttataa agcgctacca aaattttatc atattttata aaaaataaca
     8701 atccagtaaa aaatgactgg taaaaattca caaagaaacg gaaagaagca actacagaaa
     8761 atcaagcctt aaaaagagct ttttacctag accaattaaa aatcacaaag tgagcgtctc
     8821 tatccattgg tagaggggct acttggcata gtgtacatta tgtcaactat gcaattaaat
     8881 aactaactac ttcatattag cttaataaaa taatcaaaat cactagggga aaaaatattg
     8941 tataaaaaaa tttttcattt tttaaataag aatagtaaat ataaaaataa tttaagatat
     9001 ctattactat taatccagat agagcaaacc aagtaaatat tccgtgtgta aagctaaaaa
     9061 aattcatttg aatcaattca aataaaccta atatagtaac aaatatatta aaaataatag
     9121 ctgtcaatac cagtaaatta ttttgtttat tttttaaatt atcttcattt aaaatagaat
     9181 ttatgttagt actataaaat tcacaaattt tttttagtgt ctctatatct ggtgttgttt
     9241 tgttattttc ccactttgaa atagtttgac gtgtaacatt aaattttctt gccacatatt
     9301 cttgagtata ccctttt
//
