LOCUS       NZ_CP096218             3109 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Enterococcus faecium strain UCH1 plasmid pUCH1-7, complete sequence.
ACCESSION   NZ_CP096218
VERSION     NZ_CP096218.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27725314
            Assembly: GCF_023204955.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Enroth,T.J., Kirsch,J.M., McCollister,B.D., Johnson,C.N. and
            Duerkop,B.A.
  TITLE     Enterococcus faecium UCH1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Enroth,T.J., Kirsch,J.M., McCollister,B.D., Johnson,C.N. and
            Duerkop,B.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2022) Immunology and Microbiology, University of
            Colorado Anschutz Medical Campus, 12800 East 19th Ave, Aurora, CO
            80045, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2022
            Assembly Method                   :: Unicycler v. 0.5.0
            Assembly Name                     :: UCH1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 225.8x
            Sequencing Technology             :: Oxford Nanopore; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023204955.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:36:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,300
            CDSs (total)                      :: 3,209
            Genes (coding)                    :: 3,062
            CDSs (with protein)               :: 3,062
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 51 of 147
            Pseudo Genes (incomplete)         :: 92 of 147
            Pseudo Genes (internal stop)      :: 37 of 147
            Pseudo Genes (multiple problems)  :: 28 of 147
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096218.1.
            Bacteria and source DNA available from Duerkop Lab, University of
            Colorado Medical School, Aurora, CO.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="UCH1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pUCH1-7"
                     /geo_loc_name="USA"
                     /lat_lon="39.742630 N 104.842026 W"
                     /collection_date="2019"
                     /collected_by="Bruce McCollister"
     gene            complement(266..1072)
                     /locus_tag="M0E25_RS16355"
                     /old_locus_tag="M0E25_16355"
     CDS             complement(266..1072)
                     /locus_tag="M0E25_RS16355"
                     /old_locus_tag="M0E25_16355"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_080441956.1"
                     /translation="MATTKLGNTKQASRAINYAEKRATVKGGWNVNVEFAKSEMKFTRS
                     SFGKDSGIQAHTIIQSFSPEESKGLGAVTINELGVELARKVAPEFEVAVYTHTDKEHIH
                     NHIVINAVSFETGKKYQSNKAQRELVKTKNDEIARAFGLSVPEKKAEIRYTQAESEIAQ
                     DGRDSWKVDIREKVELYRTTIELERSESSFKAFMMERGINVSFRGKNVTYEHLEANRKV
                     RGAKLGGRFDKEALDKMFGSKEKELEKDKQRRYKEYLRQQGRHLEY"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1024..1419)
                     /gene="mobC"
                     /locus_tag="M0E25_RS16360"
                     /old_locus_tag="M0E25_16360"
     CDS             complement(1024..1419)
                     /gene="mobC"
                     /locus_tag="M0E25_RS16360"
                     /old_locus_tag="M0E25_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_080441957.1"
                     /translation="MASHNEKEFSVKKPKVRRVANKQISFRVNEAEYEKLQQSAKNLNM
                     SVPAFVKKKAQGAKLVRPVVEQEIALEISRNLKVLGNNANQIARRLNSGTSSDNEELNA
                     EVQRVLKGLGEIWRLLNSEIQNKRQER"
                     /name="mobC"
     gene            complement(1889..2605)
                     /locus_tag="M0E25_RS16365"
                     /old_locus_tag="M0E25_16365"
     CDS             complement(1889..2605)
                     /locus_tag="M0E25_RS16365"
                     /old_locus_tag="M0E25_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_060800859.1"
                     /translation="MSKEKARYFTFLLYPESIPDDWEEKLELIGVPIAISPLHDMDEKT
                     NKAKWTSDDVVRNGKHYKKPHYHVIYVAGNPVTTESVRLKIKRALGSKSVNKVQIITTS
                     IQNTYLYLTHESKDAIAKNKHKYKSSDIKLFNNFDIDRYNTLDVEEKDELFEVICEIVE
                     KYQIANILELGKFVRKYGAAYGLISMRVVNKVIASKTGLMRIYFDGAYQARKNGIREIE
                     LDIDLETGEIKEDEDD"
                     /name="replication protein"
     gene            complement(2677..2850)
                     /locus_tag="M0E25_RS16370"
                     /old_locus_tag="M0E25_16370"
     CDS             complement(2677..2850)
                     /locus_tag="M0E25_RS16370"
                     /old_locus_tag="M0E25_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010625631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322660.1"
                     /translation="MEEKKEMQSVTIRIPKKLYADYKQALLEQGKIVTYDLRRYMSDVV
                     EESARKETEQKK"
                     /name="hypothetical protein"
     Typing          complement(1894..2605)
                     /name="rep_cluster_2300"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.134"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cagaaatttt tagcaaaatt tcttccagcg ctaccgctcg gaaaaaaaca aaaccttgcc
       61 ctttgaaaag gtcaaatcca aactttcgct aacgctaggt tgccaaaaaa tggacgtgtc
      121 cattttttag caaaaatgag cttctgggtt tccgttacgt cgtcaactcc ttaaagcctc
      181 aaagaggggc taaaaacgca cagcatgcgt tctaagcccc tctaattcga tttaccgttg
      241 acaacactta tctcgctttc ttcgcctaat actccaaatg acgcccttgt tgtcgcaaat
      301 actccttata gcgcctctgt ttgtcttttt ccaactcttt ttctttagat ccaaacatct
      361 tatcgagcgc ttctttatca aatcttccac caagcttcgc ccctcgaacc ttcctattcg
      421 cctctaaatg ctcgtatgtg acgttttttc ctctaaacga tacatttata cccctttcca
      481 tcataaacgc cttaaacgag ctctcagagc gttctagctc tattgttgtt ctatacagct
      541 caactttctc acgtatatcc actttccacg agtctcgtcc atcttgcgct atttcagatt
      601 ctgcttgggt atatcttatt tctgcttttt tctcaggcac agagagaccg aacgctctag
      661 caatttcatc attttttgtt ttcactaact cacgttgtgc tttgtttgac tgatattttt
      721 tccccgtttc aaaactcact gcatttatca cgatatggtt atgaatatgc tccttgtctg
      781 tatgcgtata aaccgctacc tcaaattctg gcgctacttt tctcgccaac tcaaccccca
      841 actcattgat tgttacagcg cccaaacctt tactttcttc tggagaaaac gattgaatga
      901 ttgtatgtgc ttggattccc gaatcctttc caaaactcga acgagtgaac ttcatttcag
      961 acttagcaaa ctcaacattc acattccaac cacctttcac tgttgcacgt ttctccgcat
     1021 aatttatcgc tcttgacgct tgttttgtat ttccgagttt agtagtcgcc atatttcacc
     1081 cagccctttc aacacacgtt gcacttccgc atttagttct tcattgtcac tcgaagttcc
     1141 gctattcaaa cgacgtgcaa tttgattcgc attgttccct aacaccttta aatttctgct
     1201 gatctccagt gcgatttctt gctcgactac tggtcgaaca agtttcgcac cctgtgcctt
     1261 tttcttcacg aatgcaggca cgctcatatt caaatttttc gctgattgct gcaatttttc
     1321 atattcggct tcgttcactc tgaagctaat ttgcttattc gccactcgtc gaactttcgg
     1381 tttttttacc gaaaattctt tctcgttgtg gctagccatt ccttcttcgt atactcgcat
     1441 aaagtcccac tccttctttt gccacgtggc tagccacgca ccaaggttcg atttaaaatt
     1501 gtttgttttt cactcacaat tttaaatcga acgaaggagt ttggcaagcg tcgtatttgc
     1561 tagccacttc cttcggaagt ggcgcaaaca ccgcatgcaa ggtgggagct tcactccccc
     1621 ttgaccccct caaaatccac gcgccacaat acaaaaaaag gctagctcat gctagccaat
     1681 ttttgtaaag ttttgcgatt taccaaaatc aaaaaagccg gcaccctgtg ccgacacctt
     1741 tttgattttt tcgcaagtgt ataaacactt gctatgcttc cgagcggcat tcatcaccca
     1801 cctatagagg atgggcgact tctgcctgaa ttacgttaaa taatgcttaa ttccattttc
     1861 aaaataaatt acgttcctag ggtctacgtc aatcatcttc atcctccttt atctccccag
     1921 tttctaaatc aatatctaat tcaatttctc gaataccatt tttgcgtgct tgatatgctc
     1981 catcaaaata aattctcatc aaacctgttt tagacgcaat aaccttgttg actactctca
     2041 tgctaattaa gccatacgca gccccatatt tcctcacgaa tttacctaac tctaaaatat
     2101 tagctatttg atatttctca acaatttcac agatcacttc aaaaagttca tctttttctt
     2161 caacatcaag cgtgttataa cgatcaatat caaaattgtt aaatagtttt atatcagaac
     2221 ttttgtattt gtgtttattt ttggcaatag catcttttga ttcatgcgtt aaatacaagt
     2281 acgtattttg aatacttgta gttataatct gaactttatt aacacttttg cttcccaaag
     2341 ctcgtttaat tttcaatcga acactttcag tagtcacagg atttcctgct acataaatta
     2401 catgataatg tggtttttta taatgcttac cattcctaac gacatcatca ctcgtccatt
     2461 ttgctttatt tgttttttcg tccatatcat gcaaaggact aatcgcaata ggtacgccta
     2521 ttaattccaa tttttcttcc caatcatcag gaatactttc tggatatagt aaaaaagtga
     2581 agtatctagc cttttctttg ctcatttgct ataatcacct cgacagaaaa ccgatagcga
     2641 aaactcgttt cctttcaaaa aaacgcgagt tttcgcttat tttttttgtt ctgtttcctt
     2701 tctcgcagat tcttctacaa catcactcat atatcttctt aaatcataag tcacgatttt
     2761 tccttgttct aataaagctt gtttataatc agcatataac tttttaggaa tacgtatagt
     2821 cacactttgc atttcttttt tttcttccat caccagacac ccttcctata tataattcta
     2881 tataattata ataaaatcta tatacatagt caagcattac aaagaattac atttttatca
     2941 acaaaacagt aattttgttg gcgtttgttg attttttttt aattgctgta acccttgcga
     3001 gagtaaggct taaataggtt tttttctaaa tttggcactc ggcactttat ggggggtcgt
     3061 agtacggaag cgaaattcgc ttcctcgccc ccccgattcg tccaaatta
//
