LOCUS       NZ_CP096214             6303 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Enterococcus faecium strain UCH1 plasmid pUCH1-3, complete sequence.
ACCESSION   NZ_CP096214
VERSION     NZ_CP096214.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27725314
            Assembly: GCF_023204955.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Enroth,T.J., Kirsch,J.M., McCollister,B.D., Johnson,C.N. and
            Duerkop,B.A.
  TITLE     Enterococcus faecium UCH1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Enroth,T.J., Kirsch,J.M., McCollister,B.D., Johnson,C.N. and
            Duerkop,B.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2022) Immunology and Microbiology, University of
            Colorado Anschutz Medical Campus, 12800 East 19th Ave, Aurora, CO
            80045, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2022
            Assembly Method                   :: Unicycler v. 0.5.0
            Assembly Name                     :: UCH1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 225.8x
            Sequencing Technology             :: Oxford Nanopore; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023204955.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:36:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,300
            CDSs (total)                      :: 3,209
            Genes (coding)                    :: 3,062
            CDSs (with protein)               :: 3,062
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 51 of 147
            Pseudo Genes (incomplete)         :: 92 of 147
            Pseudo Genes (internal stop)      :: 37 of 147
            Pseudo Genes (multiple problems)  :: 28 of 147
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096214.1.
            Bacteria and source DNA available from Duerkop Lab, University of
            Colorado Medical School, Aurora, CO.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="UCH1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pUCH1-3"
                     /geo_loc_name="USA"
                     /lat_lon="39.742630 N 104.842026 W"
                     /collection_date="2019"
                     /collected_by="Bruce McCollister"
     gene            complement(join(5954..6303,1..31))
                     /locus_tag="M0E25_RS16220"
                     /old_locus_tag="M0E25_16220"
     CDS             complement(join(5954..6303,1..31))
                     /locus_tag="M0E25_RS16220"
                     /old_locus_tag="M0E25_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(353..556)
                     /locus_tag="M0E25_RS16225"
                     /old_locus_tag="M0E25_16225"
     CDS             complement(353..556)
                     /locus_tag="M0E25_RS16225"
                     /old_locus_tag="M0E25_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(568..1101)
                     /locus_tag="M0E25_RS16230"
                     /old_locus_tag="M0E25_16230"
     CDS             complement(568..1101)
                     /locus_tag="M0E25_RS16230"
                     /old_locus_tag="M0E25_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            complement(1551..1721)
                     /locus_tag="M0E25_RS16235"
                     /old_locus_tag="M0E25_16235"
     CDS             complement(1551..1721)
                     /locus_tag="M0E25_RS16235"
                     /old_locus_tag="M0E25_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(1737..2270)
                     /locus_tag="M0E25_RS16240"
                     /old_locus_tag="M0E25_16240"
     CDS             complement(1737..2270)
                     /locus_tag="M0E25_RS16240"
                     /old_locus_tag="M0E25_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(2291..3241)
                     /locus_tag="M0E25_RS16245"
                     /old_locus_tag="M0E25_16245"
     CDS             complement(2291..3241)
                     /locus_tag="M0E25_RS16245"
                     /old_locus_tag="M0E25_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            3917..4003
                     /locus_tag="M0E25_RS16250"
                     /old_locus_tag="M0E25_16250"
     CDS             3917..4003
                     /locus_tag="M0E25_RS16250"
                     /old_locus_tag="M0E25_16250"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107532029.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(4124..4813)
                     /locus_tag="M0E25_RS16255"
                     /old_locus_tag="M0E25_16255"
     CDS             complement(4124..4813)
                     /locus_tag="M0E25_RS16255"
                     /old_locus_tag="M0E25_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321377.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(5058..5972)
                     /locus_tag="M0E25_RS16260"
                     /old_locus_tag="M0E25_16260"
     CDS             complement(5058..5972)
                     /locus_tag="M0E25_RS16260"
                     /old_locus_tag="M0E25_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(2292..3240)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(5062..5972)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgctagccaa attttgatgt tgttcgctca taagtcccac cctttcagta cgtcctacgg
       61 acgtatcgtt cgcaatacca ttgtacctaa aaaggtacaa tgagtaaagc tcactaaaag
      121 ggtttggcaa gctctatatt tgctagccaa atacctccgt atttggtgca aataaccgct
      181 tgtcaaagga gcgcaagctc ccccttgaat ccccctcaaa atccacgctc cacaatacaa
      241 aaaaaggcta gcatttgcta gccacttttt gtaaagtttc gcttatttca ccgaaggtga
      301 aattgttgct ttgtaatatt caaacaaccc tattcttcta aaatgtatcg atttattttt
      361 ttttagactt tattatgtct aagacaacta agattaacac taaaaataaa ccagatttgt
      421 ataattgccc tccgcttagc atattaacaa ttatagcaac agctatagca aatataaaaa
      481 tacgaatagt agctatttgc ggattctttt tagtaaatgt ttttaatgat tttaatgatt
      541 tagatatcaa aaacatcact ccttaactta taacgttgaa tatctagtag ctacaactct
      601 tccagctgca tcatatcccc tttggtaata aaatccaatc cagccattac catttttgtc
      661 ccattctttt gcaactgcat tccctggaga agcacctttt ttagtccaac ttgcacctgt
      721 taacaaagca ccaattgccc aacctgggat accaaacata gaagtgctag ctttccctcc
      781 tgcccaaaag gcagcattaa tagcattagc agctactcca gtggttacag caatatttgt
      841 atattccttt ttgccccatc ttgcaactgc agctcttgtc atgatatctg aagaatttgc
      901 aaaatcttcc gcactataac catcctcata tttagttacg accataccat cttcagtgta
      961 ctcaataact acattttcag tagcattgtt ttcgtttaat gataccactt gggaattttc
     1021 tgaagcataa actatagtag ttgacggtaa aacaactgcc ccaatagtta acaaagctac
     1081 ccctaaagta aattttttca tagaatttcc tcctgataca gaaaaatata tagaaaacgt
     1141 tttctttgtg aaaatcataa aacaaaaggt ataggcttgt caatatttta tttagtaaag
     1201 ataatttata tttttgataa tgtggtatag ttagaatgca ttaggaaaat atgttatatt
     1261 aaattcttgt tgacataatg tacactatgc caagtagccc ttgtatcaac cgatacagag
     1321 gctcactttg tgattttcga ttggtctagg tcaaaagctc tttttaagct tgattttctg
     1381 cggttgcttc tttccgtttc tttgtgaatt tttaccagtc attttttact gggttgttat
     1441 ttttaataaa atatgataaa tttttggtag cgcttcataa gcgcgttgct ttttcaagaa
     1501 taaacactca ttatttttta actggctgct cgaaagagca gtctttttta ttatttatct
     1561 gtttctttta caaacaattc ttcgttagga ctgttgtaat aatttggatt caatttcaat
     1621 tcaccatgtt tattcccttt attgatcact gacaataaca tcggactgaa tcctagaatt
     1681 cctagtaaaa tagaaccgaa tagaagattt tttcttgtca tgaatgttcc ctccagttat
     1741 ttccaaagtt gccaccattt tttttcagta tcaatttgtt tgtctattgt ttctgttgct
     1801 tcagaaagag tcgttgagtc attctctgga gcttcttctg tataagtcaa ctgtagtttt
     1861 tcttgcagtt tttcgatttg tttgttggct tgtagggtga gttgttgttg ctggtcgagt
     1921 agtttttgta tatgagaatt attttgctct ctgagggcaa gttgttctcg taaaaattca
     1981 ttttcttcac gcaactgatc tagcaacttg tttgtaacca cgtttttgct tggtatatta
     2041 acatttttat cgttgtcttg ttcgtactcg tgcaacctat ctagcaacat ttggttgcct
     2101 aagttgcgag caagttgcaa tactccagaa cgcaatacaa ccttataacc acgctcattt
     2161 ttagctatca tggaagaatc taatttttgg atatgtttcg atattgcttg cctactcatc
     2221 ttaaaaagtt ctgacaattc ctttattgta aaggtttctt gtggttgcat actggttgcc
     2281 tcctaggttg ctactggaat aaattatcta aatcagataa taaacttttt ttctcttttt
     2341 tcgaaagtgc ggactctgtt tgtttttcaa gagcttcttt ctctttttct agttgtttga
     2401 tttgttggag gagttcaagt tctcgtttct tctgtttgat tttttcgtta attgttgtag
     2461 caggttcttc gtattctttt tgtttatcag ctaaagccaa tgctttattt tgttcttctt
     2521 tggttggtgc atgtttatta aaatcatctg cttttttgat ttctggttta aacgtaaatt
     2581 gatagccaac gacaggtttt cctcgccctt ttccgtattt ctttttgatt gataatcctt
     2641 tgaagattgc ggttaattcc tgtcgtatag gctttataac acgattatca acgtttgctt
     2701 ggttgttcca gtaacttttt ggaatatcta atagttcatg aaaatcctct ttagaaaaat
     2761 aagcaaaacc tttagttcga tattgtttta gtaatcgaaa catagtcttt gcatagctac
     2821 tttgtaattc gttaaattgt tggagtgaat agcgaaccca ctcatctaaa ttgtttagta
     2881 aaggtaaagc tttttcgtaa acttgtatgt ctacatatgg atctttagca gttccacgta
     2941 ttttaaactc actaaacata acgaatctat ctatatcgag cccattagca cttcttttac
     3001 caaaacgtaa atccattaat ttatcataag tcatttctaa atcattaatg aaacgattat
     3061 tagcagttgc tttataatta cttaaatctt ttagttggtc aaaagtaaga cggactgttt
     3121 tatcgccttt ttcgcgcatt ctagacacaa tagagaaaaa tagattcatt tcaactggtg
     3181 taaatttccg caacggaatg gtatttaatt catgatggta tttcacgatt tcatttgcca
     3241 aagatattcc tccttactcc ttcaatggat acagacgtta taaacaacgt tttgtctgtg
     3301 ataatgcata ttataacata cgtgagtgca aaaaaagagt ttaaaagcag acaaaatgta
     3361 gtttaaaagc agacaaaatg tagtttaaaa gcagacaaaa tgtagtttaa aagcagacaa
     3421 aatgtagttt ataaaacgct gtatccattg aaggagtaag gacacagccg acggtaaaga
     3481 ggtttaaaaa aaggtttaaa aagagattat aaaagagggt gacaaaattt cccgcctaaa
     3541 aaccacttct tttttctacc aacaggttcg cgcatttttc aaaactgcca attcaaaacc
     3601 ttgctcccta cggtcgctaa aaaagtgtga tcgctatgct ctcacattgt actgcgcagt
     3661 cgcctaaagg ctcctttgct aaaaacaagt cagtaacgga ggtgggggaa gcctctgata
     3721 gctcgtctaa gtcgttttaa gacagtcttg ggtaaaaaac cttatcttgt tcttaaagcg
     3781 ccttacaggc ggtcagggca agccctaaca acccgaaaat aagggatact taaaaacacc
     3841 acacagattt tattttgtaa ggtataataa cttgtcgcct atccgacagt cttgcgactg
     3901 gaaggaggtg tatctaatgt ttgaacggtt catttgcccc cttcttgtag aagtttgtgt
     3961 ctctttattc agttattggt tgaataaccg aaacaagaag taaaatcaat aaaggcgaca
     4021 tcaacctgaa aaaaagcccc agagaatccg acctctctgg ggctttcgtg tatctaatgt
     4081 ttgatcattt gcaggttcag tttaacatga ttgctaatca aattcaattt tgttgttgct
     4141 gatccaacca ttccttgaca ctttctagat aatttaatgc cgtttctttt ttcgttggat
     4201 tttgtatgtt tgttgcttgc tgaagcagtg tttcataacg ttcgtatgtg accgattgtt
     4261 gccgttcttt tcgttcttgt tcactggttg aaaaagggat tgtatcgcta aataagttac
     4321 aacaagcttc ttctgtctgc gtatctttaa aatattgcac gtcttttttt acttgctcat
     4381 atttttcttg tacctctttt tgctgttttt ctggtaagtt ttgataaaaa cgagtgagtt
     4441 ttttcatgtt tttatctgtt aaacgtttct cttgatccac aaaaatattg ttcatgtcct
     4501 cataacaagc ggtaaattcc tccatttttt gtgctaatgc ttgctctttt ttctttttat
     4561 cacatgctgc ttcatacttt tgagtgatat cccctaataa aatgcttctt gaaacacgtt
     4621 tattaccctc taaaacgccg aataaaccac ttaaaacgat tgataaggca aatataaccc
     4681 cgaccaataa aaatcgctta aatcgaattt tagagccttt taatgaatat cttgtttgat
     4741 aagccttata gctaagccaa ataattccta aaatactggc agaaatgagt agatagggta
     4801 aatagaatgc catgtctgtt tattctcccc ataccaacca aaaggctaat aatgttagta
     4861 ctaagcaaca taagggggca agaatatcca tcgatcgttg aattaaagcc tttttttgtt
     4921 cttggtttcg ctggtctaat agcgcttggt ggagtttttt gacttgtgaa tccatcgtag
     4981 tttgtatcct ttcttgggta taaggcgcaa gaaaggcacg ttgtctcttt atttcgtttt
     5041 cgatgtcttt taacgtttta gagcgataat tcttgttggt ggtctgttcc tcgttcaacg
     5101 tgttgttcat ggtttcttcg agtcggtcta atttccgatt gttcaggcgt aaaagttcgt
     5161 tgttctcttc gtacttcttc tgcagtttcg cgaatgctag ctcgagctct tcgtagctcg
     5221 ttggttcgtt gttgattgta ggttcgttct cgtctgtcca gctcatgtaa aatcgtctcc
     5281 ttttcgtaat cctttcctag tttgttggat cgtacttttt tctttttgtg gaccaattcg
     5341 taagtaaaat tttttccgcg attcaagcaa tgaatgccga atgttttaag tgaatcacaa
     5401 aattgggtat acgttcgagt ttttttgttt ttcattgttt gatcaatcgc aaaccgaatt
     5461 tcgtctttcc aaaccggttc atttttttct tttaatttaa tttcagacat ggttcgtttt
     5521 tcggttttct ttcctcgttc cgtgattgtt aagtcatgtt caagacaaat ctcgtcattt
     5581 aggtcataac atttgtttaa aaatgcttgc ccatgggctt gaaatttttt cccagtttca
     5641 agattaacgg agttaatgac aatgtgatta tgaatatggt ctttgtctgt atgtgtgtaa
     5701 atcgcgattt ggtggttagg ggctattttt ttcgcatact ctgcccctag ttgattggct
     5761 ttttcagcgg taatttctcc tggtcgaaag gactgaatca ataagtgtgc ttgtacacga
     5821 tcgtcttttc cataaagcgt gcgtatttgt ttcatttcac ttttggcata atcaatgtca
     5881 cagtttattc ctgttttgac ggttgctcgt ggttctgcgt aattgattgc tcggctacat
     5941 gaagtggttc gacttacttt aactgttgcc atatcttttg tagctccttt tgcatgattt
     6001 ttaggttttc ggataaacgt tgagcatcat caggagcaac gtcgtgttgg tgtgtattgc
     6061 accacttggc aatttgattg atattccctc cagctttggc taaatcgcgc gcgattgcct
     6121 gggcaatgtc tggcgccact ttcggtgtca ccagacgcgc gttttgtgcc ttggctttca
     6181 cgaaagccgg cacactcatt tgcaaagctt cagctgactg cttcagcttt gcaaattcgg
     6241 attcgctcac tcgaaaactg atttgttttg gctcggatcg ataggtatat tttttagaat
     6301 gat
//
