LOCUS       NZ_CP096202             4294 bp    DNA     circular CON 28-MAR-2024
DEFINITION  Kluyvera ascorbata strain SK plasmid unnamed1, complete sequence.
ACCESSION   NZ_CP096202
VERSION     NZ_CP096202.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27625780
            Assembly: GCF_023195735.1
KEYWORDS    RefSeq.
SOURCE      Kluyvera ascorbata
  ORGANISM  Kluyvera ascorbata
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Kluyvera.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Zuo,Z.
  TITLE     Kluyvera ascorbata,complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Zuo,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2022) Ministry of Natural Resource, First
            Institute of Oceanography, Xianxialing 6 Road, Qingdao, Shandong
            266061, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 96.31x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023195735.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 07:24:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,830
            CDSs (total)                      :: 4,705
            Genes (coding)                    :: 4,623
            CDSs (with protein)               :: 4,623
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 27 of 82
            Pseudo Genes (incomplete)         :: 57 of 82
            Pseudo Genes (internal stop)      :: 15 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096202.1.
            Bacteria and source DNA available from Kluyvera ascorbata strain
            isolated from marine sediments.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Kluyvera ascorbata"
                     /mol_type="genomic DNA"
                     /strain="SK"
                     /isolation_source="Pacific Ocean"
                     /db_xref="taxon:51288"
                     /plasmid="unnamed1"
                     /geo_loc_name="Pacific Ocean"
                     /collection_date="2019-11"
     gene            19..1305
                     /locus_tag="MY052_RS24155"
                     /old_locus_tag="MY052_24155"
                     /db_xref="GeneID:85164589"
     CDS             19..1305
                     /locus_tag="MY052_RS24155"
                     /old_locus_tag="MY052_24155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899670.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_239509525.1"
                     /db_xref="GeneID:85164589"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWKRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTRKGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            2081..2272
                     /locus_tag="MY052_RS24160"
                     /old_locus_tag="MY052_24160"
                     /db_xref="GeneID:85164590"
     CDS             2081..2272
                     /locus_tag="MY052_RS24160"
                     /old_locus_tag="MY052_24160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_163219962.1"
                     /db_xref="GeneID:85164590"
                     /translation="MTKQQKTALNMAKFIQAQSLLLLEKLNELDLDTEADLCEALHEDA
                     EHLFRSLTTRIDGLQEKY"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2272..2679
                     /locus_tag="MY052_RS24165"
                     /old_locus_tag="MY052_24165"
                     /db_xref="GeneID:85164591"
     CDS             2272..2679
                     /locus_tag="MY052_RS24165"
                     /old_locus_tag="MY052_24165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440114.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_035897801.1"
                     /db_xref="GeneID:85164591"
                     /translation="MAQVSISEAARLTGKSRTTLHRLIKAGELSTCHGERNAKMLDTSE
                     LLRVFGALRGLPDEQVVEQVTGQRVTASLSENEQVVRALKQEVEHLKSLVSAQNSHIDS
                     LKQAMQLLEHKQSASDPQKVPAKPWWQFWKG"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3343..3588)
                     /locus_tag="MY052_RS24170"
                     /old_locus_tag="MY052_24170"
                     /db_xref="GeneID:85164592"
     CDS             complement(3343..3588)
                     /locus_tag="MY052_RS24170"
                     /old_locus_tag="MY052_24170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_163219960.1"
                     /db_xref="GeneID:85164592"
                     /translation="MKKWLTEFFDDMRYIWGEFVREVNSATTDDDTDALDAPGAEYSAE
                     WHATPDFDPQKAINNIDNVYTHDTLHAHIYGHDYDH"
                     /name="hypothetical protein"
     Typing          complement(1347..1846)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.604"
                     /coverage="99.0"
                     /evalue="1.02e-154"
ORIGIN
        1 atgttttcac ttaaaaatat gttctccctg aacctccagc gatcagccat ttcgtatgac
       61 gcaatttgtc agaataagcc tgtcattgag ttcagtccgg agggggtgat aaataaagcc
      121 agcccgcttt ttctctccac tatggggtac agggctgatg aaataatcgg tcaccaccat
      181 cgtatattct gcccgccctc gctggtaagt tcaccggaat acagccagtt ctggaagcgc
      241 cttgccaggg gagaaagctt tagtggcaag tacctgcggc tggcaaaggg aaatcgttcc
      301 gtatggcttg aagccagtta tattcccgtt tctgacaggc gcggccgtgt tatcagagtc
      361 attaaaattg ctgccgatat ttctgagcgc gtgcattctg ctcttgagca ggaagcagtc
      421 gtaaatgcca taaaccgttc tatggccatc atcaccttca atcctgaagg gatcgtgctt
      481 gaagcaaatg aaaatttcgt caatgccact ggctataagc gggatgaaat cataggtaag
      541 catcatcgtc tgttctgtgc cgagacgctt tacaaaagtg atgaataccg acatttctgg
      601 gagagtctga atcaaggtga gtttttttct ggtctgtttc cacgccttaa ccgtcaggga
      661 gaccctctgt ggttccgtgc aacttataac cctgtcttta acagtgatgg gcagctttat
      721 aaaattgtga aattcgctac agatgttacg gctgatgtcc tgcgaaacca gagagagcaa
      781 gaggctgctg tgcatgcatg ggatatggcc gtgcagaccc gagaaagtgc gcagaacggt
      841 gccaatgtta ttgaaaatag catccttatg attgacagaa ttgcgcaggg gatgggggct
      901 gtctccaccg atatctcacg gctcaacaat cagtctgaaa gtattgacga tatggtggaa
      961 accatccgga agtttgccat gcagacaaga cttattgcac tgaatgccgc gattgaagca
     1021 gcaagggctg gcgcatccgg aagaagtttt gcggttgttg ccgcagaagt gcgtaatctt
     1081 gcggcgagcg tcagtagcgc gactgaagaa attgagcaag tcgtggccag caatagtcaa
     1141 ctggccaagg atgttctctg tggcattgaa aacagcctga tgaacactcg gaaaggggtt
     1201 acactcatgc gcgaagcggg tgaagttatg accagcattc agaggaatgc tgcaggggtt
     1261 gagactgcgg ttaaggatgt cgccatttca gttaaagccg gataggccat ggtgcatatc
     1321 cccatatctg tcgaacatca cagatgatct tcttgagatc ctttccgttg cgtcgtaatc
     1381 tcttgctctg aaaacgaaaa aaccacccgg caaggtggct ttttcgaagg ttcgcaagag
     1441 ttgacgctct tttgaaccgc ggtaactggc ttggaggagc acagtcacca aaactgtcct
     1501 ttcagtgtag ccttatcgca cccgaaactt caagactctc ctcctctaaa cctgttacca
     1561 gtggctgctg ccagtggcgc tttgtcgtgt ttttccggat tggactcaag ttgatagtta
     1621 ccggataagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc cagcttggag
     1681 cgaactgcct tcccggaacc gagtgtcagg cgtggaatga gataaacgcg gccataacag
     1741 cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agccgccagg
     1801 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat ttgaacgtca
     1861 tattttgtga tgctcgtcag gggggcgaag cctatgaaaa aacgcccgga tgggcgtgag
     1921 cttatcgtcc ttgtcctgtt ttttggttac gcttgtcgtc tatcttactt gtcactttga
     1981 aagaccggtc cgctcgccgc agacaagtga ccgagcgtag cgagcgaacg gtcgaggaag
     2041 cggaaaagaa ccggatataa cctggagcac tgacgcagtg atgactaaac aacagaaaac
     2101 agcactgaat atggcgaaat tcattcaggc gcaatctctg cttttgctcg agaagctcaa
     2161 cgaactggat ctcgataccg aggccgactt gtgcgaagca ttacatgagg atgcggaaca
     2221 cctttttcgt agcctcacga cacggattga tggtcttcag gagaagtact aatggctcag
     2281 gtatctatca gtgaagcagc tcgtcttacc gggaaaagtc gtaccacgct gcacaggttg
     2341 attaaagcag gtgagttgtc cacctgtcac ggcgaacgaa atgcaaaaat gttagataca
     2401 tcagagttat tgcgcgtatt tggtgcgtta cgtggtcttc ctgatgaaca ggttgttgaa
     2461 caggtcactg gacagcgtgt tacagcatca ttgtctgaga atgaacaggt ggtcagggcg
     2521 ttgaaacagg aagttgaaca cctgaaatcg ctggtttctg ctcagaattc tcatatcgat
     2581 agtctgaaac aggcgatgca gctcctggag cataaacaga gcgcttctga cccgcagaag
     2641 gttcctgcaa agccatggtg gcagttctgg aagggctgat tacccctgaa attctgcctt
     2701 tccagtacga ttttttgcat gcaaaaaatc tgtttatcct gtcagggcgg cgccccgaca
     2761 cccgccagcg cctcaccgcc acgcaataaa ttgcgtgccc gttcaccgct tatgtttcct
     2821 gtctgatcga taagtcttct ggtggctgaa aacgaccaga aaatcgtgtc acacctgacg
     2881 cactatacag tactgctccg aaagccttgt gctgcaaggc tttcgggcgc tacaccccca
     2941 ctgtctgaag tgtagcgaaa agcccctaaa aacgcagtta tctcattgtt ttatatggaa
     3001 taaaaagtgt agcgatgagc gctacacttt ttatcttaaa cttcgcttta attgcatgtt
     3061 ttataatgtt tttctttgag tttggggtgt agcgcggatg cagtttattt ggatggatgc
     3121 ttgtttcagg tctgcggctt tttaagcgta aattctggat gtgatccggg ctattttttc
     3181 gcggggtgtc ctgttcccga gtccgaaggc tttatcccct cgatgaatcg cgcttgatct
     3241 tctattacat cgggtcgctc gaccaggaag tcgggggatg cttttttcgt gctggccagg
     3301 tcgcgccaca ggactctttt cctgggtgct tttgcatgat gttcagtggt catagtcgtg
     3361 gccatagatg tgcgcgtgaa gggtatcgtg ggtatacacg ttatcaatgt tgtttatcgc
     3421 cttttgcgga tcaaaatccg gcgttgcgtg ccactctgcc gagtattccg caccgggcgc
     3481 atccagagca tccgtatcgt catctgtcgt ggcgctgttt acttcgcgga cgaattcacc
     3541 ccagatataa cgcatatcgt cgaaaaattc ggtgagccac tttttcatat cgtgtcctgg
     3601 ttgcgctgtg tgtacatcaa cgagtgtagc taccgggaga agatacaccg gaaagggata
     3661 aaaatgcctg attgatggaa ttttccgtcc gtaacgtggt gtcagtcatt ggcgtgcata
     3721 acgattcacg cctgtaaaac gccccagcgc gattctgggg cgttttcagg agggaagata
     3781 tgtggttttg cggcttcggc aggaaaatcg cgtacagccc gtctgagcgg tgcgggcaag
     3841 gggtgttatg ttaaaagccg aggtctggcg gatacggaaa aaattatgcc ggccgggctt
     3901 atgcccgcgg caatccggcc gatacttaaa ttatccttca caaaggatgt gctgcgatcc
     3961 aggcagttga gtgttttccg gctcccggat gggagccatt tttttgcctt ctgatgcctg
     4021 ttaatgaatg cggagctgcg ctggttcttc caagattgat agacgccagc ttctctgctg
     4081 gagccggcgg atttgccgta aatatgaaca ttttcttatt gttcagcccg ctaaagatgg
     4141 cgggtttttt acgcgtgaaa tccggagccg gacatcatgc cggcatggct gaagagcggg
     4201 ctttttcccg ccctgtcgca gtaatatata aagccacata aggagtagcc gataaccatg
     4261 gcagtactta cattatccgg gcaatctgat tcat
//
