LOCUS       NZ_CP096197             3458 bp    DNA     circular CON 15-AUG-2023
DEFINITION  Enterobacter roggenkampii isolate C3-17 plasmid unnamed10, complete
            sequence.
ACCESSION   NZ_CP096197
VERSION     NZ_CP096197.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27117675
            Assembly: GCF_023195715.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ota,Y. and Saito,R.
  TITLE     Enterobacter roggenkampii from sink in nursing home
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Ota,Y. and Saito,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2022) Molecular Microbiology, Tokyo Medical and
            Dental University, 1-5-45, Yushima, Bunkyo-ku 113-8510, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 448.7x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/15/2023 06:31:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,722
            CDSs (with protein)               :: 4,722
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 39 of 102
            Pseudo Genes (incomplete)         :: 53 of 102
            Pseudo Genes (internal stop)      :: 28 of 102
            Pseudo Genes (multiple problems)  :: 17 of 102
            Pseudo Genes (short protein)      :: 1 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096197.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /isolate="C3-17"
                     /isolation_source="sink"
                     /db_xref="taxon:1812935"
                     /plasmid="unnamed10"
                     /geo_loc_name="Japan: Tokyo"
                     /lat_lon="35.70153025 N 139.76415553 E"
                     /collection_date="2021-12"
                     /collected_by="Tokyo Medical and Dental University"
     gene            222..1526
                     /locus_tag="M0769_RS24650"
                     /old_locus_tag="M0769_24650"
     CDS             222..1526
                     /locus_tag="M0769_RS24650"
                     /old_locus_tag="M0769_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899670.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_047352831.1"
                     /translation="MRSLKNLFSLNRQRSAISYDAICQNKPVIEFSPEGVIHKASPLFL
                     STMGYRADEIIGHHHRIFCPPSLVSSPEYSHFWQRLARGESFSGKFLRRAKGNRSVWLE
                     ASYIPVTDRRGRVTRVIKIAADISERVHSALEQEAVLNAINRSMAIITFNPEGIVLDAN
                     ENFVNATGYKRDEVIGKHHRLFCTETLYKSDEYRQFWERLNQGEFFSGQFPRINRQGDP
                     LWFRATYNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRGSAQNGA
                     NVIENSILMIDRIAQGMGAVSTDVSRLNNQSESIDGMVETIRKFAMQTRLIALNAAIEA
                     ARAGASGRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLSGIENSLMTTREG
                     VTLMREAGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            complement(1769..1882)
                     /locus_tag="M0769_RS24655"
                     /old_locus_tag="M0769_24655"
     CDS             complement(1769..1882)
                     /locus_tag="M0769_RS24655"
                     /old_locus_tag="M0769_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048339450.1"
                     /translation="MHEPPVQSDRCACSGNYQLESNPERHDKTPLAAATGN"
                     /name="hypothetical protein"
     gene            complement(3033..3218)
                     /locus_tag="M0769_RS24660"
                     /old_locus_tag="M0769_24660"
     CDS             complement(3033..3218)
                     /locus_tag="M0769_RS24660"
                     /old_locus_tag="M0769_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032448787.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEDDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2244..2414
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.22"
                     /coverage="98.0"
                     /evalue="5.6599999999999995e-68"
     Typing          2905..2986
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.941"
                     /coverage="96.0"
                     /evalue="4.19e-26"
     Typing          complement(1774..2320)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.075"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 cttaagcgcg tgacgaaaaa attaccgcct gccctctaag gccggcgcgg cggcggccga
       61 taactgaggt gcctgaaacc aggttttaac tctgtgacac gttgtcttcc gctccctgtc
      121 cgggagcttt ttttctgcca ccggcagggc gggcatgatg gtatccgcgt cctcggggat
      181 aaccatggca gtccttacat tatccgagca atctgatgta tatgcgttca cttaaaaatc
      241 tgttctccct gaaccgccag cgctcagcca tttcgtatga cgcaatttgt cagaataagc
      301 ctgtcattga attcagtccg gagggggtga tacataaagc cagcccgctt tttctctcca
      361 ctatggggta cagggctgat gaaataatcg gtcaccacca tcgtatattc tgcccaccct
      421 ctctggtgag ttcaccggaa tacagccatt tctggcagcg ccttgccagg ggcgaaagct
      481 ttagtggcaa gttcctgcgg cgggcaaagg gaaatcgttc cgtatggctt gaagcaagtt
      541 atatccccgt tactgaccgg cgcggccgtg tgaccagagt cattaaaatt gctgcagata
      601 tttctgagcg cgtgcattct gcgcttgagc aggaagcagt cctaaatgcc ataaaccgtt
      661 ctatggccat aatcaccttc aatcctgaag ggatcgtgct tgatgcaaat gaaaatttcg
      721 tcaatgccac tggctataaa cgggatgaag tcatcggtaa gcatcatcgt ctgttttgta
      781 ccgagacgct ttacaaaagt gatgaatacc gacagttctg ggagaggctg aatcagggtg
      841 agtttttttc tggtcagttt ccacgtatta accgtcaggg agaccctctg tggttccgtg
      901 caacttataa ccctgtcttt aacagtgacg ggcagcttta taaaattgtg aaattcgcta
      961 cagatgtcac ggctgatgtc ctgcgaaacc agagagagca ggaggctgct gtgcatgcat
     1021 gggatatggc cgtgcagaca cgaggaagtg cgcagaacgg tgcaaatgtt attgaaaata
     1081 gcatccttat gattgacaga attgcgcagg ggatgggagc tgtctccacc gatgtctccc
     1141 ggctcaacaa tcagtctgaa agtattgacg gtatggtgga aaccatccgg aagtttgcca
     1201 tgcaaacaag acttattgca ttgaatgccg cgattgaagc agcaagggcg ggcgcatccg
     1261 gaagaagttt tgcggttgtt gccgcagagg tgcgtaatct tgcggcgagc gtcagtagcg
     1321 cgactgaaga aattgagcaa gtcgtggcca gcaatagtca actggctaaa gatgttctca
     1381 gtggcattga aaacagcctg atgaccaccc gggaaggggt cacactcatg cgtgaagcgg
     1441 gtgaagtgat gaccagcatt cagaggaatg ctgcaggagt tgagactgcg gttaaggatg
     1501 tcgccatttc agttaaagcc ggataggcca tggtgcatat ccccatatct gtcgaacatc
     1561 acagatgatc ttcttgagat cctttccgtt gcgtcgtaat ctcttgctct gaaaacgaaa
     1621 aaaccgccca gcaaggcggt ttttcgaagg ttctcagagc agcaactctt tgaaccgtgg
     1681 taactggctt atgaggagcg cagtaaccaa atctgttctt tcagtttagc cttaacaggc
     1741 gcataacttc aagactaact cctctaaatc agttaccagt ggctgctgcc agtggcgttt
     1801 tgtcgtgtct ttccgggttg gactcaagtt gatagttacc ggaacaggcg cagcggtcgg
     1861 actgaacggg gggttcgtgc atacagtcca tcctggagcg aactgccttc ccggaaccga
     1921 gtgtcaggcg tggaatgaga taaacgcggc cataacagcg gaatgacacc ggtaaaccga
     1981 aaggcaggaa tgcgggggag cacgagggag ccatcagggg gaaacgcctg gtatctttaa
     2041 gccgcatcgg gtttcgccac cactgatttg agcgtcagat tttgtgatgc ttgtcagggg
     2101 ggcggagcct atggaaaaac ggcttcgccg tggctttatc gcttccctgt taagtttctt
     2161 cctggcatct tccaggaaat ttccgccccg ttcgtaagcc atttccgctc gccgcagccg
     2221 aacgaccgag cgcagcgagt cagtgagcga ggaagcggca tatctccgtt attacatatt
     2281 ctgctgacgc gccggtgccg ccttctttct cctgccacat gaagcacttc actgacatcc
     2341 gcatccgtgc caacatatta agccagtata cactccgcta gcgctacgtg actggtccgg
     2401 cgctgcgccc ggaacccgcc tgtaccggtt cagcagccgt tccggcctga ctgcaatttt
     2461 tttcattcct gcccgctatc ctgtaaaaat ttcttctgcg ttgccgttaa caggaggtaa
     2521 gccatgtttg gttttcttcc ggagccctga tgactcttag tgatgatgag cgacacctgc
     2581 ttgtgtcgcg ttgtttcggg ctggctcagg catccccccc ggcacgcctg agccatgatg
     2641 ctggatgcat accggcagat tttgtctgaa accgaaccgg ccgtaagggc ggtcatgctc
     2701 gaatttcttg aatccgttcg cattcattac atttcgtcct gagtattttc tgaatttctg
     2761 tccatactta aacgaggcgg ccgtcacgga cggtggtcgt cgttgcatga tgctgttatg
     2821 gatgagtgaa agtaatctag tctaacttga cttaccttcc gggcagctcg cccggttttt
     2881 tttgcctgtt cccggggaac cactccggac agtatgtgca tttgtaatac cgcgcacgcg
     2941 ccggcatcac aaatacttag gtcagggctg cgccccgaca cccgccatcg cctcacggcc
     3001 gtgcactgaa ttgcgcaccc gttcaccgat ttttacgcat catcttcgcc tatctgtgcg
     3061 ttcagcatgg cgtaaagcgc ttcggcctga tcgtgcagat gctcacagca gccggctgcc
     3121 tcatccagat ccatctgttc gagccgctcg aggaggagga gtgtctgtga gcggataaat
     3181 ttggccatgt tgagggcggc ggattcctgt ctggtcactg gtgattttcc ccgtctggtg
     3241 atgatttttg cgtgaaaagt gcatgccgtc attctggtgc atagtcatgc atgccgttaa
     3301 aatttaacag gcgcgtttct gacgggttcc ggggtggttt gttgcctgtt ttgccggttt
     3361 cccgtcagaa acgctcccag accgcctgag gcggtgcggg caacggatgt tatggtaaat
     3421 tcgtcaggct gttgcattaa gtttcatgaa caatgcaa
//
