LOCUS       NZ_CP096195             4664 bp    DNA     circular CON 15-AUG-2023
DEFINITION  Enterobacter roggenkampii isolate C3-17 plasmid unnamed8, complete
            sequence.
ACCESSION   NZ_CP096195
VERSION     NZ_CP096195.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27117675
            Assembly: GCF_023195715.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ota,Y. and Saito,R.
  TITLE     Enterobacter roggenkampii from sink in nursing home
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Ota,Y. and Saito,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2022) Molecular Microbiology, Tokyo Medical and
            Dental University, 1-5-45, Yushima, Bunkyo-ku 113-8510, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 448.7x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/15/2023 06:31:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,722
            CDSs (with protein)               :: 4,722
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 39 of 102
            Pseudo Genes (incomplete)         :: 53 of 102
            Pseudo Genes (internal stop)      :: 28 of 102
            Pseudo Genes (multiple problems)  :: 17 of 102
            Pseudo Genes (short protein)      :: 1 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096195.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /isolate="C3-17"
                     /isolation_source="sink"
                     /db_xref="taxon:1812935"
                     /plasmid="unnamed8"
                     /geo_loc_name="Japan: Tokyo"
                     /lat_lon="35.70153025 N 139.76415553 E"
                     /collection_date="2021-12"
                     /collected_by="Tokyo Medical and Dental University"
     gene            complement(568..2124)
                     /locus_tag="M0769_RS24605"
                     /old_locus_tag="M0769_24605"
     CDS             complement(568..2124)
                     /locus_tag="M0769_RS24605"
                     /old_locus_tag="M0769_24605"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589620.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_032993376.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLY
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVLV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPLKSVTGNLIILLSHPIYD
                     KGGNYIGYVGGSIYLKKHNLFGDVLSRHFFKNNTEISIVSDDGNVIFNNDKSAVDRPMV
                     MSDDLKSKLTASDRGEGAFFSSGHRYLLGYASMKNTAWNVLVYSRADSVTTILIAYAKS
                     VLVLLIIIIIVFSLISYFIASSISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTYIKVMMERVDFLNEVALKDPLTGVNNRLGFLSKIQNHKRDSGYSVIVIDVDFF
                     KEINDSFGHGVGDEVLVSLAHIINSCCRSEDVVCRFGGEEFVVFLPNTSVVTAKCVAER
                     IRSVIESTVFPNNLRVTISAGVATQDDPLGRIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            complement(2470..2685)
                     /locus_tag="M0769_RS24610"
                     /old_locus_tag="M0769_24610"
     CDS             complement(2470..2685)
                     /locus_tag="M0769_RS24610"
                     /old_locus_tag="M0769_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_012565230.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMLRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2689..3174)
                     /locus_tag="M0769_RS24615"
                     /old_locus_tag="M0769_24615"
     CDS             complement(2689..3174)
                     /locus_tag="M0769_RS24615"
                     /old_locus_tag="M0769_24615"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012565228.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQNTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<3209..3643)
                     /locus_tag="M0769_RS24620"
                     /old_locus_tag="M0769_24620"
                     /pseudo=""
     CDS             complement(<3209..3643)
                     /locus_tag="M0769_RS24620"
                     /old_locus_tag="M0769_24620"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3852..4175)
                     /locus_tag="M0769_RS24625"
                     /old_locus_tag="M0769_24625"
     CDS             complement(3852..4175)
                     /locus_tag="M0769_RS24625"
                     /old_locus_tag="M0769_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4289..4461)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="9.14e-86"
     Typing          2..286
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.289"
                     /coverage="89.0"
                     /evalue="2.46e-105"
     Typing          complement(2367..3862)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3859..4175)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="1.81e-61"
ORIGIN
        1 taaagatacc aggcgtttcc ccctggtggc tccctcgtgc gctctcctgt tcctgccttt
       61 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttat ctcattccac gcctgacact
      121 cggttccggg aaggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagcc
      181 cgactaccac gttgtcccgg taactatcat cttgagtcca acccggaaca gacacgaaaa
      241 atcgccagtg gcggtagcca ttagtaagag gattcacgga gcggagtagt agagtgttct
      301 acagagttct tgaagtggtg gcctgactgc ggctacactg gaaggacagt tttggtacct
      361 gcgctccgtg aaagccagtt accttcggaa gaaagagttg gtagctctga attccggcaa
      421 acgaaccacc gttggtagcg gtggtttttt tgtttacagg gtatgagatt acgacgacat
      481 cgaagggata acaagaggat ccgcttcttg cccactcttc gccacaaaaa aaacgacaca
      541 ctagcgcgta aagcatcatc aaaaaactca aatagcgcta tatacaatca ctttattacg
      601 cccctccctt ttggcttgat aaagagcatc gtcagcattt ttcagcaaaa aatcgatcct
      661 accaagagga tcatcttgtg ttgccacacc agcagatatg gtgacccgca agttattagg
      721 gaagacagta ctttcaataa cactcctaat acgctccgca acacacttag ctgttaccac
      781 agatgtattc ggcaggaaaa caacaaactc ttcgccacca aaccgacaaa caacatcttc
      841 actacggcaa caggaattaa taatatgagc caaagaaaca agcacctcat ctccgacccc
      901 atgcccgaaa ctgtcattta tctctttaaa aaaatcaaca tcaattacaa taactgaata
      961 ccctgaatcc cgcttatgat tctgtatttt acttaaaaac cccaatctat tgttcacgcc
     1021 ggtaagggga tctttaagcg ccacctcatt tagaaaatca actctttcca tcattacctt
     1081 aatatacgta aaaagcgcct ttttaagcct atcagcttcg gcataccaag cattgatagc
     1141 tttaatataa actagcgaag ctccgatatc tttagcattg gtagatatag caagcttttc
     1201 taaaggaagt gaaatactgg aggcaatgaa atatgaaata agtgaaaaca caataattat
     1261 tataattagc agaacaagta cacttttagc ataagcaata agaatagtag tcacactgtc
     1321 agcacgacta taaaccaaca cattccaagc agtatttttc atactagcat aaccaagaag
     1381 atatctatgc cccgaagaaa aaaatgcccc ctcacctcta tcagacgcag ttaactttga
     1441 tttcaaatca tcagacataa ccataggccg atcaacagca cttttatcgt tattaaagat
     1501 cacattccca tcatcactaa caatgctaat ttcagtgttg ttcttgaaaa aatgcctgct
     1561 caaaacatcg ccaaataaat tatgtttttt tagatatata gacccaccga cataccctat
     1621 gtaattgcca cccttatcat aaataggatg tgacaacaaa ataatcaaat tcccagtaac
     1681 tgattttaac ggtgaagata tgaatggttt cttttcctca atcgccaatt tgctggcact
     1741 tgaattcaag cgggtaccaa ctaaatcaag agattctggc gaagtcgcca gaacaacagc
     1801 atcattacta acaacaagaa ctgaattaaa cataccagac tgcaatctta acctatccgc
     1861 ctcatcttta agtgagtcaa tatcattagt tgattttatt ttttttgctc catatgctaa
     1921 ttcaccttga gccatttcaa tataaccact cattacatct gacattttca tagcgtacga
     1981 tgaattagca taaagcaaat ctttttctat attttcgcgc tgaaaaacca taataacggc
     2041 aatcagaaaa atagaagtta acaaaatccc accaacggac aaggttatga ctatcggacg
     2101 aaggtgaggc tttttaattc tcataaaaat acactccata taataaaaaa tgccgcattt
     2161 accataacat cccttatcag caccgccccg gacgggttcg gaacaaatgc ggcgtcagcc
     2221 cggtaaaacc ggcagcaaac tctccgctaa accgcgcatt ctgcgccaga taaatttcac
     2281 ccggctgcat ggctatgcag tcgggtgaat ggctccggct ttcagcgcag atgtccgccg
     2341 aaagccccgt tttatcaggt tatcacagtg aagggctgtt atacgtccgc tgtcgctgag
     2401 atcgctccag ctccagcgct ttctgatgct gcaccacgcg ctcctgctcc cggtgcagct
     2461 cgacggcatt cagtcgctcg acaaccggcg cagctgctcg ctcaaactct gcacctgcct
     2521 gctcaagccc gtgacgcgct cgctcagcgc tgcgttctcc cgtagcataa gaccagacat
     2581 cgtccgccat tccgcgaagg cgctctccca ctcgtccagc cttttcgagt agtcctgctg
     2641 tagctgctct aatgcgctca gcaactgttt ttccagctcc gtcatgtgct atttccccgc
     2701 cagtatcatc cagctcgaat cctctccgaa ccgtcccgct tccgggttca ccttcacgca
     2761 gcggcgtctc tgctcgccgc aggtcgagag ctgcacgccg ctgttcctct ctgacagcat
     2821 ggcctgcgtg ctcttctgct cccggatggt cgtgtagttg tcgagtatct gctgcccctg
     2881 ccaccacaga atgcccgcgc tcgaggcgat cagcagcgcg gagaccagga cgatggtcag
     2941 ccacgtctgg ctgaccatgc gcaccatgcc tttcgtgcgc tggctcatgg ctgaggacag
     3001 tttccggtcg tggtcgagga tggcggcgct gattctcttc gcgctttcgt tcagttccgc
     3061 ttttacagat ttctcgtgct cgctgaatgc ggctttcagc atctcgccgg tattctgctg
     3121 ctgcgctttc gatttctgct ctaagtcctt cgccagcgtt aaaagactgt tcataaatgg
     3181 ctcccttcag tcggatgttt cgccccccgt ccgggtcggc aatgctgata ctgctttttg
     3241 tggtgcggac cacctcaaaa cctgcgcttt ccagcgcctc agtgacatcc tgacgggttt
     3301 tcagctcccc ggatgaggca agagcaagca gaccgcgtgt aatcgcttct gcagcctcct
     3361 gcttcgcttt aggcagtgca gacggtgtga ccagtgcccg ccggttctcc ggtgcgttcg
     3421 ggtcgtgcag ccccagtctg ccgttcacga tggtctgcca ggcatcgatg cgtggacggt
     3481 cggcgcgatc gtaatacggc tggagccgtc tgccggtcag cagctccgtg ttcgggatca
     3541 gaaaattcag ctcaagccgc cccttgtcac gatgctccac ccacaggatg ctgtactggt
     3601 ctttatcaag tccgggcatc agaacccgct caaagcttgc catcagcttt tcccgctggc
     3661 cggaaggtaa atccgcttcc gcaaacgaca gtaccccgga ggtgtatttc ttaacgtaag
     3721 gcgacgcgtc aatcagctcc cggacttctt ccggcttccc ctgcaggacg gtggcgcctt
     3781 cacgctgccg gtctttaccc agcagatagt ccaccggccc ggcgccaccg ccgcgaccgc
     3841 gtggatgaaa tttaacgatc atcgtccgtt cctttctcaa gaacggcgtg ccgcagccgc
     3901 tcaagtccgg catcgatggc catcagcgcc gcaacaatct gcaccctgtc atggccggta
     3961 ccgccgccgg cgttaacacg gcgggctatc tggttcaggt tattgcccat gccggcaagc
     4021 tggcgcagca gcgccggcga gattgagggg agctttccgg aacgtgccgg cttttcgtcc
     4081 aggcaggtct gccgcatcca cgccgccagc tgcctgccgt cacagcgctc aagcagccgc
     4141 cggtgctcgt cctccgtcac ccacatggtg agcatcttgt tccgtttctc tgacaaaccg
     4201 gtctcctgat aacgccggga cgggcgggaa aacctttccc ggtcggcacg gggttggaca
     4261 agccgcaggc gcgtcaggct tttggcgggt ttcggggcgc agccctgaac cagtcacgta
     4321 gcgctagcgg agtgtatact ggcttactat gttggcacgg atgcgaatat cagtgaagtg
     4381 cttcatgtgg caggagaaaa atgcagcagc ggagcgtcag cagaatatgc gatacaggat
     4441 atattccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4501 accggcttac aggcggggcg gaaatttcct gggagatgcc agaaagaagc ttaacaggga
     4561 agcgataagg ccgcggcgaa gccgtttttc cataggctcc gcccccctga caagcatcac
     4621 gaaatctgac gctcaaatca gtggtggcga aacccgacag gact
//
