LOCUS       NZ_CP096193             4938 bp    DNA     circular CON 15-AUG-2023
DEFINITION  Enterobacter roggenkampii isolate C3-17 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP096193
VERSION     NZ_CP096193.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27117675
            Assembly: GCF_023195715.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Ota,Y. and Saito,R.
  TITLE     Enterobacter roggenkampii from sink in nursing home
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Ota,Y. and Saito,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2022) Molecular Microbiology, Tokyo Medical and
            Dental University, 1-5-45, Yushima, Bunkyo-ku 113-8510, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 448.7x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/15/2023 06:31:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,722
            CDSs (with protein)               :: 4,722
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 39 of 102
            Pseudo Genes (incomplete)         :: 53 of 102
            Pseudo Genes (internal stop)      :: 28 of 102
            Pseudo Genes (multiple problems)  :: 17 of 102
            Pseudo Genes (short protein)      :: 1 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096193.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /isolate="C3-17"
                     /isolation_source="sink"
                     /db_xref="taxon:1812935"
                     /plasmid="unnamed6"
                     /geo_loc_name="Japan: Tokyo"
                     /lat_lon="35.70153025 N 139.76415553 E"
                     /collection_date="2021-12"
                     /collected_by="Tokyo Medical and Dental University"
     gene            complement(518..1822)
                     /locus_tag="M0769_RS24550"
                     /old_locus_tag="M0769_24550"
     CDS             complement(518..1822)
                     /locus_tag="M0769_RS24550"
                     /old_locus_tag="M0769_24550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437803.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_007899670.1"
                     /translation="MFSLKNMFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFL
                     STMGYRADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLE
                     ASYIPVSDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEAN
                     ENFVNATGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDP
                     LWFRATYNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGA
                     NVIENSILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEA
                     ARAGASGRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREG
                     VTLMREAGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            complement(2733..2948)
                     /locus_tag="M0769_RS24555"
                     /old_locus_tag="M0769_24555"
     CDS             complement(2733..2948)
                     /locus_tag="M0769_RS24555"
                     /old_locus_tag="M0769_24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2955..3446)
                     /locus_tag="M0769_RS24560"
                     /old_locus_tag="M0769_24560"
     CDS             complement(2955..3446)
                     /locus_tag="M0769_RS24560"
                     /old_locus_tag="M0769_24560"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_016355729.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGRQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESNDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<3481..3915)
                     /locus_tag="M0769_RS24565"
                     /old_locus_tag="M0769_24565"
                     /pseudo=""
     CDS             complement(<3481..3915)
                     /locus_tag="M0769_RS24565"
                     /old_locus_tag="M0769_24565"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4124..4447)
                     /locus_tag="M0769_RS24570"
                     /old_locus_tag="M0769_24570"
     CDS             complement(4124..4447)
                     /locus_tag="M0769_RS24570"
                     /old_locus_tag="M0769_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007899679.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTAKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGVGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4563..4735)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="9.14e-86"
     Typing          2..477
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.166"
                     /coverage="95.0"
                     /evalue="4.83e-143"
     Typing          4660..4938
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.908"
                     /coverage="90.0"
                     /evalue="9.73e-111"
     Typing          complement(2633..4134)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.692"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4131..4447)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="3.03e-59"
ORIGIN
        1 ataaagatac caggcgtttc cccctggtgg ctccctcgtg cgctctcctg ttcctgcctt
       61 tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac
      121 tcagttccgg gaaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
      181 ccgaccgctg cgccttatcc ggtaactatc aacttgagtc caatccggaa aaacacgaca
      241 aagcgccact ggcagcagcc actggtaaca ggtttagagg aggagagtct tgaagtttcg
      301 ggtgcgataa ggctacactg aaaggacagt tttggtgact gtgctcctcc aagccagtta
      361 ccgcggttca aaagagcgtc aactcttgcg aaccttcgaa aaagccacct tgccgggtgg
      421 ttttttcgtt ttcagagcaa gagattacga cgcaacggaa aggatctcaa gaagatcatc
      481 tgtgatgttc gacagatatg gggatatgca ccatggccta tccggcttta actgaaatgg
      541 cgacatcctt aaccgcagtc tcaacccctg cagcattcct ctgaatgctg gtcataactt
      601 cacccgcttc gcgcatgagt gtaacccctt cccgagtgtt catcaggctg ttttcaatgc
      661 cacagagaac atccttggcc agttgactat tgctggccac gacttgctca atttcttcag
      721 tcgcgctact gacgctcgcc gcaagattac gcacttctgc ggcaacaacc gcaaaacttc
      781 ttccggatgc gccagccctt gctgcttcaa tcgcggcatt cagtgcaata agtcttgtct
      841 gcatggcaaa cttccggatg gtttccacca tatcgtcaat actttcagac tgattgttga
      901 gccgtgagat atcggtggag acagccccca tcccctgcgc aattctgtca atcataagga
      961 tgctattttc aataacattg gcaccgttct gcgcactttc tcgggtctgc acggccatat
     1021 cccatgcatg cacagcagcc tcttgctctc tctggtttcg caggacatca gccgtaacat
     1081 ctgtagcgaa tttcacaatt ttataaagct gcccatcact gttaaagaca gggttataag
     1141 ttgcacggaa ccacagaggg tctccctgac ggttaaggcg tggaaacaga ccagaaaaaa
     1201 actcaccttg attcagactc tcccagaaat gtcggtattc atcacttttg taaagcgtct
     1261 cggcacagaa cagacgatga tgcttaccta tgatttcatc ccgcttatag ccagtggcat
     1321 tgacgaaatt ttcatttgct tcaagcacga tcccttcagg attgaaggtg atgatggcca
     1381 tagaacggtt tatggcattt acgactgctt cctgctcaag agcagaatgc acgcgctcag
     1441 aaatatcggc agcaatttta atgactctga taacacggcc gcgcctgtca gaaacgggga
     1501 tataactggc ttcaagccat acggaacgat ttccctttgc cagccgcagg tacttgccac
     1561 taaagctttc tcccctggca aggcgctgcc agaactggct gtattccggt gaacttacca
     1621 gcgagggcgg gcagaatata cgatggtggt gaccgattat ttcatcagcc ctgtacccca
     1681 tagtggagag aaaaagcggg ctggctttat ttatcacccc ctccggactg aactcaatga
     1741 caggcttatt ctgacaaatt gcgtcatacg aaatggctga tcgctggagg ttcagggaga
     1801 acatattttt aagtgaaaac atatgaatca gattgcccgg ataatgtaag tactgccatg
     1861 gttatcggct actccttatg tagcgttata tattactgcg acagggcggg aaaaagcccg
     1921 ctcttcagcc atgccggcat gatgtccggc tccggatttc acgcgtaaaa aacccgccat
     1981 ctttagcggg ctgaacaata agaaaatgtt catatttacg gcaaatccgc cggctccagc
     2041 agagaagctg gcgtctatca atcttggaag aaccagcgca gctccgcatg cattaacagg
     2101 catcagaagg caaaaaaatg gctcacatcc gggagccgga aaacactcaa ctgcctggat
     2161 cgcagcacat cctttgtgaa ggataattta agtatcggcc ggattgccgc gggcataagc
     2221 ccggccggca taattttttc cgtatccgcc agacctcggc ttttaacata acaccccttg
     2281 cccgcaccgc agaaacgggg ttggctgcgt ttccggtgcc agacgcgaga aagtggcgac
     2341 agacatgaca aaaaaacacc aaaaacgcag ccgctggata ttacactgat gcatagtcat
     2401 gcagccagat gcatgcagcg taaagtcaca ctaaaatcag cgcgaacgcc ttaaaaaccg
     2461 gcaaatgcct ctccggcaac tcacacagct ccatcaccag gtaaaaacag ttcagcaggg
     2521 cttcaataac gttgagaatt ttcatcactt cctccgggat aagtcactac cattccggta
     2581 gtcagcctat ttagcaggaa cgcgatgagc ccttcttcgt gcgggctgtc acctcatctt
     2641 cggaccatcg aaactcttct gcttctgtcg gagcgtcagc tccagctcgt tctgacgctg
     2701 atactccagt gcagccttct cctcgatggc attcagttcc cggacaagcg gcgcagctgc
     2761 tcgcttaaac tcagcacctg cgtgctcaag tccgacacgc gctcgttcag caccgcgttc
     2821 tcccgttgca taagtccaca catgctccgc cattccgcga aggcgctctc ccattcgtcc
     2881 agcctttgcg agtagtcctg ctgtagctgc tctaatgcgc tcagcaactg cttttccagc
     2941 tctgtcattt tccgctactc ctgtttcagg cggatccagg tcgtcccgtc gtaggggatg
     3001 gcttcggctt gcatccccgg cggcaggata aggaacctcc ggccgtcgtt gctctcctga
     3061 tagcgtactc cccacgtccg ggcggtcatt ttcgccaggg tatcttcctg ctgacgaaga
     3121 tgtgtgtaat tgtcggttat ctgccgtccc tgccaccaca gaatgctccc gctcgtggcg
     3181 atcagcagcg ctgacaccat caggatggtg agccatgtcc tcccggccgt cagcaacacg
     3241 ctccgccggt ttttctccat cgcctcgctc atactctgct cgtgggcgct gatggcgtcg
     3301 ctgattctcc tcgcgcttgc gttcagttcc gctctgacag actgctcgtg ctcgctgaat
     3361 gcggctttca gcatctcgcc ggtactcttc tgctgcgctt tcgattgctg ctctaagtcc
     3421 ttcgccagcg ttaaaagact gctcatagat ggctcccttc agtcggatgt ttcgcccccc
     3481 gtcagggtcg gcaatgctga tactgctttt cgttgtacgc accacctcaa aacctgcacc
     3541 ttcaagagcc tcagtgacgt cctgacggct tttcagctcc ccggacgagg ctaagctcag
     3601 cagtccacgg gtgatggctt ctgcggcttc ctgcttcgtt ttcggcagtc cggccggcgt
     3661 gaccagcgcc cggcggtttt ccggcgcgtt cggatcgtgc agccccagtc tgccgttcac
     3721 gatggtctgc caggcatcga tgcgcggccg atcggcgcgg tcgtagtacg gctggagccg
     3781 cctgccggtc agcagctcgg tattggggat cagaaagttc agctccagac gccccttgtc
     3841 ctcgtgctcc acccacagaa tgctgtactg atctttatca agtccgggca tcagaacccg
     3901 ctcgaagctg gccatcagtt tttcccgctg gccgggaggc agatcggcct ccgcaaacga
     3961 caggacgccg gaggtgtatt tcttcacgta aggcgacgcg tcgatcagct cccggacttc
     4021 ttccggcttc ccctgcagga cgctggcgcc ttcacgctgc cggtctttac ccagcagata
     4081 gtccaccggc ccggcgccac cgccgcgacc gcgtggatga aatttaacga tcatcgtccg
     4141 ttcctttctc aagaacggcg tgccgcagcc gctcaagccc ggcatcgatg gccatcagcg
     4201 ccgccacaat ctgcacccgg tcatgaccgg taccgacgcc ggcgttaaca cggcgggcta
     4261 tctggttcag gttgttgccc ataccggcaa gctggcgcag cagcgccggc gagatcgaag
     4321 gaagttttgc ggtacgagcc ggcttttcgt ccaggcaggt ctgccgcatc cacgccgcca
     4381 gctgcctgcc gtcgcagcgc tcaagcagcc gcctgtgctc atcctccgtc acccacatgg
     4441 tgagcatctt gttccgtttc tctgacaaaa cagtctcctg ataaccccgg gacgggcggg
     4501 aaaacctttc ccggtcggcg cggggttgga caagccgcag gcgcgtcagt ggctgttagc
     4561 gggtttcggg gcgcagccct gaaccagtca cgtagcgcta gcggagtgta tactggctta
     4621 ctatgttggc acggatgcga atatcagtga agtgcttcat gtggcaggag aaaaatgcag
     4681 cagcggagcg tcagcagaat atgcgataca ggatatattc cgcttcctcg ctcactgact
     4741 cgctgcgctc ggtcgttcgg ctgcggcgag cggtaccggc ttacaggcgg ggcggaaatt
     4801 tcctgggaga tgccagaaag aagcttaaca gggaagcgat aaggccgcgg cgaagccgtt
     4861 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg
     4921 gcgaaacccg acaggact
//
