LOCUS       NZ_CP096192             6183 bp    DNA     circular CON 15-AUG-2023
DEFINITION  Enterobacter roggenkampii isolate C3-17 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP096192
VERSION     NZ_CP096192.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27117675
            Assembly: GCF_023195715.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Ota,Y. and Saito,R.
  TITLE     Enterobacter roggenkampii from sink in nursing home
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Ota,Y. and Saito,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2022) Molecular Microbiology, Tokyo Medical and
            Dental University, 1-5-45, Yushima, Bunkyo-ku 113-8510, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 448.7x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/15/2023 06:31:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,722
            CDSs (with protein)               :: 4,722
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 39 of 102
            Pseudo Genes (incomplete)         :: 53 of 102
            Pseudo Genes (internal stop)      :: 28 of 102
            Pseudo Genes (multiple problems)  :: 17 of 102
            Pseudo Genes (short protein)      :: 1 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096192.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /isolate="C3-17"
                     /isolation_source="sink"
                     /db_xref="taxon:1812935"
                     /plasmid="unnamed5"
                     /geo_loc_name="Japan: Tokyo"
                     /lat_lon="35.70153025 N 139.76415553 E"
                     /collection_date="2021-12"
                     /collected_by="Tokyo Medical and Dental University"
     gene            complement(613..948)
                     /gene="mobC"
                     /locus_tag="M0769_RS24510"
                     /old_locus_tag="M0769_24510"
     CDS             complement(613..948)
                     /gene="mobC"
                     /locus_tag="M0769_RS24510"
                     /old_locus_tag="M0769_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_080202507.1"
                     /translation="MDKRTKEIKIRLTESEHERLLARCDRAHLAEWLRSLGLGEHVLRR
                     RRVPDVDPALLRQVTGIGNNLNQIARKLHGLDAIPAYQQVSLLAVLTSLDRQLSELLET
                     QRDRQNS"
                     /name="mobC"
     gene            complement(<1211..1321)
                     /locus_tag="M0769_RS24515"
                     /old_locus_tag="M0769_24515"
                     /pseudo=""
     CDS             complement(<1211..1321)
                     /locus_tag="M0769_RS24515"
                     /old_locus_tag="M0769_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041205629.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /name="IS30 family transposase"
     gene            complement(1419..3347)
                     /locus_tag="M0769_RS24520"
                     /old_locus_tag="M0769_24520"
     CDS             complement(1419..3347)
                     /locus_tag="M0769_RS24520"
                     /old_locus_tag="M0769_24520"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="WP_200925414.1"
                     /translation="MIKLKKTFILSIYFLLMSTILAYGLGYFKKPGVISYIMIAGFFIC
                     YFVSSRSKLCSIMLFTIFSISSIYFPTGITYGLLSKDIMLPLIGTNSMEIFGYILAMKY
                     YFGISILFVILNIPFIFMKKKGGSSLVYIFFIFLISFVMYGFSNSNVYLNDHGIIRVAL
                     RSIIEINHDNIDFYKELNDNSKLKSERSNKNFDPEIRILVIGESVRRDYMSLYGYPYQT
                     TPYLDSSNGIFIDGMTSAAPNTEQSLGRALFKTYPDQNKIDHGINIISLAKKAGYLTYW
                     VSNQGRSSNGDSKLYKLSKLGNESYFMKKGDFLSSHEGDEDMLPIIEKIVRSNDNKKVI
                     IIHMMGSHEPVCSRLTNFTPQFKINNEAGCYVASIEKLDGFIKNIISIVNGKSYKLMYF
                     SDHGLYVDKDKIFHDVDLYDEYQVPFFYLSSNMKSRVLLNKKVSGLNILDFYASFINVK
                     SNYTDSIYNIEDYNKIPESKDPIIYWVNYKKLSALHEKQRPLNPSYILGQDGINEHILN
                     INKPISATNNCIGYIDNYSYLYPSNKSITLTQGWIASVQDKKPVIGRVGLIINHNGNYS
                     FIEGSRVNRIDVYNALNNLDPSNIEYGISVKAKKRENIEMYLGYINNENKAYICTNHAP
                     LIREFGQ"
                     /name="phosphoethanolamine transferase"
     gene            complement(3501..4412)
                     /locus_tag="M0769_RS24525"
                     /old_locus_tag="M0769_24525"
     CDS             complement(3501..4412)
                     /locus_tag="M0769_RS24525"
                     /old_locus_tag="M0769_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004088970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_200925413.1"
                     /translation="MTNQALDLFNERLPRKPYFTDELQFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRPGAAIDWSDRNAPAPTLTIMNPENGHAHLLYALKTAVRTAPDGKMK
                     PLKYAAAVERALCQKLDADTGYSGLICKNPNHDHWQLAVWQPELYTLDWLADSLDLSAA
                     NDKEILPDYGLGRNCTLFDKTRKWAYRAIRQGWPEYDQWLKACYERTCAYNLQFLTPLD
                     ENEVAGIAKSIAKWTHKNFSEESFSDFVLKTHTSELQSKRGRIGGAIGGKISKGGGRPS
                     LGEPWISLGISRRKFFYDKRNK"
                     /name="replication initiation protein"
     gene            4694..4933
                     /locus_tag="M0769_RS24530"
                     /old_locus_tag="M0769_24530"
     CDS             4694..4933
                     /locus_tag="M0769_RS24530"
                     /old_locus_tag="M0769_24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="WP_064179603.1"
                     /translation="MPVSAMRHKKTVSVTIEPALLHQAREAGINLSATLTTALKHQIRE
                     SEAERWKRANRKAIQELNRITDEHGLLSDDHRTF"
                     /name="type II toxin-antitoxin system CcdA family
                     antitoxin"
     gene            4933..5241
                     /locus_tag="M0769_RS24535"
                     /old_locus_tag="M0769_24535"
     CDS             4933..5241
                     /locus_tag="M0769_RS24535"
                     /old_locus_tag="M0769_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="WP_037427062.1"
                     /translation="MQFMVYRNNSNNRAYPYLLDVQSDIIDELNTRLVIPLFLLDEVNG
                     RPARRLTPVLNVDGEDFLVMTHEMASVRQSQLGEEVVSVREHRQAIKNALDFLLDGF"
                     /name="CcdB family protein"
     gene            complement(5345..5581)
                     /locus_tag="M0769_RS24540"
                     /old_locus_tag="M0769_24540"
     CDS             complement(5345..5581)
                     /locus_tag="M0769_RS24540"
                     /old_locus_tag="M0769_24540"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_037427059.1"
                     /translation="MTETEKLLLSALERLQDDYETRQQAWQASYDSLQTMFTTTSAALM
                     RSDQACQHLSGQVESLRQQVMSLTEAVSRLTRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5642..6127)
                     /locus_tag="M0769_RS24545"
                     /old_locus_tag="M0769_24545"
     CDS             complement(5642..6127)
                     /locus_tag="M0769_RS24545"
                     /old_locus_tag="M0769_24545"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_200925412.1"
                     /translation="MSGILDLAKAFEETSKQQAQSTGESVKSEFVKLEQSISEALKSSG
                     KNISDAIHEQNTRQTTLLRRSVTIALIWILVCGVLLIGILSGILWFQGTLIASRLDEMN
                     SYSQQLTDLKAKSGAGVEILTDDTRKNKYYVVLPKSAKGVELYTSKNDKQVVTYTAK"
                     /name="MbeB family mobilization protein"
ORIGIN
        1 ctgcttttcg tttctcgcac cactgtcaac ccgccgtttt ccagcgccct gaccatatcc
       61 tcacggtcgc ggatcatccc ctgtgccacc atcgtttcca gcgagtccgt aatggtctgc
      121 gccgccttct gacggtcgcc gggaaggtca tttcccggtg tcagggttcg ccttcgggcg
      181 ggatcatgag ggtcatcaag attcaggcgg tcattggtca gagtctgcca ggcattgacc
      241 ctgggacggt cagcccggtc atagtagggt tgcaggcgtt tccagctctg caattcgatg
      301 ttcgggatca caaaattcag ctccagcctg tccttgtccc ggtgctccac ccaaaggcag
      361 gcgtactggt cgccatccag accgggcaac agtgtctgct cgaactcctc catcagacgc
      421 tctttctcat cgtcagggag gttttcttcc cggaacgaca acaccccgga ggtgtaggcg
      481 cgggcaaaat cacaaccatc aatcagttgc ctgatacgct caggctcacc gcgcagcacc
      541 accgcttttt ccctgtctcg ttctttcccc agcaggtaat ccaccgggcc actgcctgca
      601 cccgcacccc ggctatgaat tttgacgatc acgctgtgtc tccagaagct cagataactg
      661 gcgatcaaga ctggtcagta ccgccagaag agagacctgc tgataggccg ggatagcgtc
      721 cagaccatgc agcttacggg ctatctgatt aaggttattt ccgatgccgg tcacctggcg
      781 aagcagtgcc ggatctacat caggcacacg ccgacgacgt aatacatgtt cacccagacc
      841 gagcgagcgc agccattcag cgagatgagc acgatcacaa cgtgccagta gtcgctcatg
      901 ttcactctca gtcaggcgga tttttatctc tttcgttcgc ttgtccatga cactccgctg
      961 agaaaacttt cgcacccaga gtgcgaattt tcgcagtgga tgccaggggt ttcggggggc
     1021 ggcgagcccc ctgaaccagt cacagaagga accctcgcag aggggacgct gtgtgtactg
     1081 gctataatct ataatgcagt atgaaagatt ctatgctggc tgcaaaaaaa aagccctctt
     1141 taaagagggc tgttatctga tataggcttt gtttagtgca aaattcacct gatccatgct
     1201 agggatgaca tgtagattta ctgatgccaa cacgcttact gatctcagtc agactaatct
     1261 tctcaccaaa caacagtcct atctggtatc gtacgacttc agttaactgc cgttacttca
     1321 tgttactcgc tcatctgtgg taggaggaat gggatagcct atcggtagtt accccttccg
     1381 cctacattct ccactgtcat gatgacagag cgttgcactt attgtccgaa ttcgcgtatc
     1441 agtggcgcat gattagtaca tatataagct ttattttcgt tatttatata acctagatac
     1501 atttcaatat tttctctttt tttagcttta acactaatac catactctat gtttgatggg
     1561 tctaggttat tcaacgcatt ataaacatcg attctgttta ctcttgagcc ttctataaaa
     1621 gaataattac cattatgatt aataatcaag cccactctac cgataacggg tttcttatct
     1681 tgcactgatg caatccatcc ttgagtaaga gtaattgatt tgtttgatgg gtatagatat
     1741 gaatagttat caatgtagcc aatgcaatta tttgtcgcag aaatcggttt atttatattt
     1801 aaaatatgct cattaatgcc atcttgtcca agtatatagc ttgggttcaa aggtctttgt
     1861 ttttcatgta gtgctgatag ttttttgtaa ttaacccaat aaatgatagg atcttttgat
     1921 tctggtattt tattataatc ttcaatatta taaatactgt ctgtataatt gctttttaca
     1981 tttataaatg atgcgtaaaa atctaatata ttcaaaccac tcactttctt attaagaaga
     2041 actctacttt tcatatttga agataaataa aagaaaggaa cttggtactc atcatatagg
     2101 tctacatcgt gaaatatttt atccttgtcc acatacaaac catgatctga gaaatacatt
     2161 aatttataac ttttcccatt aacaatactt attatatttt tgatgaatcc atcaagcttc
     2221 tcaattgagg ctacatagca tcctgcttca ttatttattt tgaactgagg cgtaaaattt
     2281 gttagccttg agcatactgg ttcatgtgac cccatcatat gtattattat taccttttta
     2341 ttatcattac ttctgactat tttttctatt attgggagca tgtcttcatc gccttcatgg
     2401 cttgaaagaa aatctccttt tttcataaag tatgattcat tacctaattt tgataattta
     2461 taaagcttac tatcaccatt tgatgatcta ccttgattac taacccagta cgttaaatac
     2521 ccagcttttt ttgctaatga gatgatattt atgccatggt ctattttatt ttgatcagga
     2581 tatgttttaa acaaagctct gccaagtgat tgttctgtat ttggggcagc tgatgtcatt
     2641 ccatcaataa aaataccatt tgatgaatct aagtaaggag tcgtttgata tgggtaacca
     2701 taaagagaca tgtaatctct acgaactgac tcccctataa ctaagattct aatctctgga
     2761 tcaaagtttt tatttgacct ctcagatttt aatttactat tgtcatttag ctctttataa
     2821 aaatcaatat tgtcatgatt tatctcaata attgacctta atgcaactct aattatccca
     2881 tgatcattca aatatacatt tgaatttgag aaaccgtaca tcacgaaaga aattaaaaaa
     2941 ataaaaaaaa tgtatacgag cgagcttcca cctttctttt tcatgaaaat aaatggtata
     3001 tttaaaatca caaatagaat tgaaattcca aaatagtact tcatggcaag tatataacca
     3061 aatatctcca tggaatttgt tccaataaga ggaagcatta tatccttact taaaagacca
     3121 tatgttattc ctgttgggaa ataaatcgag gatattgaga atatagtaaa aagcattatc
     3181 gagcataatt tactcctcga tgaaacaaag tagcaaataa aaaagccagc tatcattata
     3241 tatgatatga cacctggctt tttaaagtaa ccaagaccat aagcaagtat ggtactcatt
     3301 agtaagaaat atattgataa aataaatgtt ttttttaatt taatcatccg attgaaatct
     3361 caacaattaa taataactaa agatagtaag ttctctgaaa agctgtcagc tacagagatg
     3421 ttagcctcga atctgattta ttaaacaacg ccacaataat gtttacctca agcatccctg
     3481 ataatatttt ataaatccac ttacttattt cgtttgtcat aaaaaaactt acgcctacta
     3541 atgcctaatg aaatccatgg ttctcccagt gatggtcgtc cacctccttt agatatttta
     3601 cctccaatag ctcctccaat acgacctcgt tttgattgca actcggaggt atgagttttc
     3661 agtacaaaat ctgaaaatga ttcctctgaa aaatttttat gagtccactt agcaatgctt
     3721 ttagctattc cagccacctc attttcatct aatggggtta gaaattgcag attataagca
     3781 caggtacgtt cgtaacaggc tttcagccac tgatcatact caggccagcc ctgacggatt
     3841 gcgcgatacg cccatttacg ggttttatcg aacagggtgc agttacggcc taaaccataa
     3901 tcgggtagga tttctttgtc gttcgccgca ctcagatcga gtgagtccgc taaccagtcc
     3961 agcgtgtaaa gttccggctg ccacacggca agctgccagt ggtcatggtt cggattcttg
     4021 cagattaacc ccgaatagcc ggtgtcagcg tccagttttt gacacagcgc acgctcaacc
     4081 gctgccgcat acttcagcgg cttcattttt ccgtcaggtg cggtgcgtac tgcggttttc
     4141 agtgcgtaca gcaaatgggc gtgaccattt tcgggattca tgatggtcag tgtgggcgcg
     4201 ggggcgttgc ggtcgctcca gtctatcgcc gcaccagggc ggtcaacgtc aaacccgagc
     4261 cagaacatcg cgtgtggctg gttgaactgg atatatttcg caagaatggc gcgctctttc
     4321 ccggcaatgc gtacgccgaa ctggagttcg tcggtaaaat agggtttgcg gggaagccgc
     4381 tcgttaaaaa ggtcgagtgc ctggttagtc aatctcgcct gcgcttatgg cgggctgttg
     4441 tttcgcacgg ttcatgtgct agtatccttc ttaggttttg tgcctgcgcc ctcgttaatc
     4501 atgtccttcc gccaagtcga gcatgattac gggggcgtcg tcttttctgc cgcttaacgt
     4561 tacacacccc gccgtatttt gtcacctgtc accgtcagtt attgcctgaa gcaacagacc
     4621 attaccttcc gggtaaaaaa cgcgtacact ggcaacacgt gctatataca cgtgtaaaaa
     4681 tcaggagatc atcatgcccg tatcagcgat gcgccataaa aaaaccgtca gtgtgacaat
     4741 cgagcctgcg cttttgcatc aggcccgcga agccgggatt aatctgtctg ccacgctcac
     4801 caccgccctg aaacatcaga tccgcgagtc agaagcggag cgctggaaac gcgccaaccg
     4861 caaggcaatc caggagctca accgtattac ggacgagcac ggcttactct ctgacgatca
     4921 caggacgttc tgatgcagtt catggtttac cgcaataaca gcaacaaccg ggcttatccc
     4981 tatctgctcg atgtacagag cgatattatc gacgaactga atacccgcct ggtgatcccg
     5041 ctgtttctgc tggatgaagt gaatggacgg cccgcccgcc gcctgacccc ggtgctgaat
     5101 gtggacggtg aggattttct ggtcatgaca catgaaatgg ccagcgttcg ccagtcacag
     5161 ctaggggaag aggtggtgag cgtccgggag caccggcagg ccatcaagaa tgcgctggat
     5221 tttctgctcg acggtttctg agtttacctt tccatcccgt gatcgtgtcc ccggctgcgt
     5281 ccgggggcaa atgagcgcgt tttctgtcgt tcctgtttct gcgccagctc cctgtccagt
     5341 ctggctacct gcgcgtcaag cggctgacgg cttcggttaa gctcatgacc tgctgccgca
     5401 agctctccac ctgcccgctc aagtgctgac acgcctggtc gctgcgcatc agcgcggcgg
     5461 aagtagtggt gaacatggtc tgcaagctgt cgtaactggc ctgccatgcc tgctgtcgtg
     5521 tctcgtaatc gtcctgtaag cgctctaacg cactcagcag cagtttttcg gtctcggtca
     5581 tggatttgct tgtccttatc gctaaaaacg ccgtatggcg cttctgtggc aactgacggg
     5641 gctatttcgc ggtgtaggtc actacctgtt tgtcgttttt cgaggtgtac agctcgacgc
     5701 ccttcgcgct cttcggcagc accacgtaat acttgttctt ccgggtgtcg tccgtgagta
     5761 tttccacccc cgcccccgat ttcgctttca ggtcggtaag ctgctggctg tagctgttca
     5821 tctcgtccag ccgtgaggcg atgagtgtcc cctgaaacca cagtatcccc gacagaatac
     5881 cgatcagcag cacaccacaa accagtatcc atatcagcgc tatcgtgacc gagcgccgca
     5941 gcagggttgt ctgccgggtg ttctgctcgt ggatagcgtc gctgatgttc ttcccgcttg
     6001 atttcaaggc ctcgctgatg gattgctcga gcttcacaaa ctcgcttttc acgctctccc
     6061 cggtactctg tgcctgctgc ttcgaggtct cctcgaaggc tttcgctaaa tctaaaatcc
     6121 cgctcatata acgctccttt taaccggata tttcgtccac cgtccgggtc ggcaatgctg
     6181 aga
//
