LOCUS       NZ_CP096191            12879 bp    DNA     circular CON 15-AUG-2023
DEFINITION  Enterobacter roggenkampii isolate C3-17 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP096191
VERSION     NZ_CP096191.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27117675
            Assembly: GCF_023195715.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 12879)
  AUTHORS   Ota,Y. and Saito,R.
  TITLE     Enterobacter roggenkampii from sink in nursing home
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12879)
  AUTHORS   Ota,Y. and Saito,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2022) Molecular Microbiology, Tokyo Medical and
            Dental University, 1-5-45, Yushima, Bunkyo-ku 113-8510, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 448.7x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/15/2023 06:31:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,939
            CDSs (total)                      :: 4,824
            Genes (coding)                    :: 4,722
            CDSs (with protein)               :: 4,722
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 39 of 102
            Pseudo Genes (incomplete)         :: 53 of 102
            Pseudo Genes (internal stop)      :: 28 of 102
            Pseudo Genes (multiple problems)  :: 17 of 102
            Pseudo Genes (short protein)      :: 1 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096191.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12879
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /isolate="C3-17"
                     /isolation_source="sink"
                     /db_xref="taxon:1812935"
                     /plasmid="unnamed4"
                     /geo_loc_name="Japan: Tokyo"
                     /lat_lon="35.70153025 N 139.76415553 E"
                     /collection_date="2021-12"
                     /collected_by="Tokyo Medical and Dental University"
     gene            join(12597..12879,1..101)
                     /locus_tag="M0769_RS24435"
                     /old_locus_tag="M0769_24435"
     CDS             join(12597..12879,1..101)
                     /locus_tag="M0769_RS24435"
                     /old_locus_tag="M0769_24435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070559064.1"
                     /translation="MSAFSMLCDRLRHEATLAKSTGAGDALAQVLETGGAAQTFAPIGA
                     PVASPTESVHRARAQSIQDAAFLTEMKQAGVAPADLVMLDGVLASSRGLPDASRAPAFA
                     AVRAQVQQQASLSPAARGINWDE"
                     /name="hypothetical protein"
     gene            89..463
                     /locus_tag="M0769_RS24440"
                     /old_locus_tag="M0769_24440"
     CDS             89..463
                     /locus_tag="M0769_RS24440"
                     /old_locus_tag="M0769_24440"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_137470439.1"
                     /translation="MGRVKPMPRHLQGKTRMPLAGGCYSAGHRLALISLLPVLEARPGD
                     RDDGERSKLALYQESLIKAGQPHAFALHEPSLYRWGAYLKSRRARYLQETDAGVAAASD
                     ALDRHAFGRAIGAVLGADDE"
                     /name="hypothetical protein"
     gene            complement(621..1262)
                     /locus_tag="M0769_RS24445"
                     /old_locus_tag="M0769_24445"
     CDS             complement(621..1262)
                     /locus_tag="M0769_RS24445"
                     /old_locus_tag="M0769_24445"
                     /inference="COORDINATES: protein motif:HMM:NF016255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_070559060.1"
                     /translation="MTETTRPILTIKRRNRTVYVPPEKAAKSEPQPAERTTSKAKVQPK
                     AKAPQKPAQKPTAAKPKKTPEQVAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGAELNMPPEADRKAVRRILGIVTGTSSYQQS
                     LIDQCMRYDLDGNPTEPATPEEAENAKARLTEIKAFRRKARQAKGEKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(1259..2548)
                     /locus_tag="M0769_RS24450"
                     /old_locus_tag="M0769_24450"
     CDS             complement(1259..2548)
                     /locus_tag="M0769_RS24450"
                     /old_locus_tag="M0769_24450"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157698538.1"
                     /translation="MNDDNDTTEATQPSTEALKCVGAAERFPDIMPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCPEHREPLRWNGGDGHAGE
                     VELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTSSVCADCKAAP
                     LVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPEFAQNETRSLAKIPV
                     ILPQGEQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLALSVLAAVGRWDYINDI
                     IRRIDRNRGDAATLIALADHIRTNRPERDL"
                     /name="hypothetical protein"
     gene            2904..3113
                     /locus_tag="M0769_RS24455"
                     /old_locus_tag="M0769_24455"
     CDS             2904..3113
                     /locus_tag="M0769_RS24455"
                     /old_locus_tag="M0769_24455"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088765954.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGDPVLKRFIADQRRPGRAPYGVR"
                     /name="hypothetical protein"
     gene            3475..4281
                     /locus_tag="M0769_RS24460"
                     /old_locus_tag="M0769_24460"
     CDS             3475..4281
                     /locus_tag="M0769_RS24460"
                     /old_locus_tag="M0769_24460"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_248352137.1"
                     /translation="MARREDADRRKADKAAADAVRMLAKGDGNPAYTPYWRNKTTIARR
                     GGVSLPPLPADLRVGGLFRSFEPRAYWSRCLLVPAYRLTDDRQLVGDALEAICGVCSAG
                     GKTGDKFSPAGTQKAGRFYPMPGFMDVLDTSPHAPYSPLFVCEGFATAVALHHLTKGKV
                     AVCAAFSIAGFGSCVASLRRFWPDVDVMLVPDHGEAYRLAHKAAYGDEREPVPTIPGAA
                     VVSFDGLDRFTPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERR"
                     /name="hypothetical protein"
     gene            4507..4965
                     /locus_tag="M0769_RS24465"
                     /old_locus_tag="M0769_24465"
     CDS             4507..4965
                     /locus_tag="M0769_RS24465"
                     /old_locus_tag="M0769_24465"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088765952.1"
                     /translation="MKSKTELREAATARSLAVIATEMSEWSLDGFSHLKLPNFSAGERQ
                     QTLSGSVVVDRPPFDYEWAGTEKFNTLATRALQVKLPASRERNYAWLCGVERETLATAL
                     LVELFSVTGCVAFAGLGKVADLAFLTLDESEAGQIRAAMLQWLDGAAA"
                     /name="hypothetical protein"
     gene            complement(5220..5804)
                     /locus_tag="M0769_RS24470"
                     /old_locus_tag="M0769_24470"
     CDS             complement(5220..5804)
                     /locus_tag="M0769_RS24470"
                     /old_locus_tag="M0769_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004589499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061351424.1"
                     /translation="MDITISDATYSRLAAQAKGFETPEQVINRLIDVVEIDHDTRPELF
                     FNPEGEADFKKALLKSKQAKVEMFKRDGSCETGVWNARAISEESSVRANIWSGYLRGWK
                     EKGIVRAVFTVQEVPDLPGAAESVQWNDYTISRTFSGGISVQRNGMPVTIVKPFLRDVA
                     HDLKISLLNSNGNEMNTRQLGAAIIKKLNNS"
                     /name="hypothetical protein"
     gene            complement(6251..6742)
                     /locus_tag="M0769_RS24475"
                     /old_locus_tag="M0769_24475"
     CDS             complement(6251..6742)
                     /locus_tag="M0769_RS24475"
                     /old_locus_tag="M0769_24475"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061351425.1"
                     /translation="MAIGDDAIRDAFYAFTQQAEEMDDKGLPQEVWETPCFTYLMTKKQ
                     FNQMKVVCQRNGWDIPASPAIPITWAMFRHVLSARNSKDKLSWQECAEILATAFSVQSN
                     VYVNRDYSEQTIVLNAVRRINVAGAGFFAMAIVDVSENNLAPVTAYHATEAKCKAIQRG
                     "
                     /name="hypothetical protein"
     gene            complement(6807..7298)
                     /locus_tag="M0769_RS24480"
                     /old_locus_tag="M0769_24480"
     CDS             complement(6807..7298)
                     /locus_tag="M0769_RS24480"
                     /old_locus_tag="M0769_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070558888.1"
                     /translation="MREKTKTLTVRLPVSLIERIEQNAARTGATRTDAVIEALNAGLGT
                     KPEGMDTKLLAAIEALRAEVRALSVGGKPARQTAQQTAPQSAQQPARQIVPRGADPRFQ
                     AVSDEADQLIVSMTAENATLPAIASALNAAGHRTATGQEWNTSRVRDYRHKLRESGLLN
                     "
                     /name="hypothetical protein"
     gene            7826..9121
                     /locus_tag="M0769_RS24485"
                     /old_locus_tag="M0769_24485"
     CDS             7826..9121
                     /locus_tag="M0769_RS24485"
                     /old_locus_tag="M0769_24485"
                     /inference="COORDINATES: protein motif:HMM:NF017116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="WP_119879886.1"
                     /translation="MSQIDENSGTAADNVTPIAAAKVGRPSLQKKYPTTAFPVELEINY
                     RKGRYSSSLENVVAAMRCHSVTGIQLAFDEFFAEEGAFVNGQWYPLSDNLVTELRLRLA
                     AADFEKVTKDNARDAMFYVCDENKFDSAQRWLTAQEWDGKPRVSYFFERCFKCQGDPEY
                     LAAVSLYTWTALAGRVMNPGIKADMVPLLYGRQGARKSTGVRLMSPFPDSYSSFNLNDK
                     DDSLVRRMRGCLIGELEELRGLHGRDSESVKAFFSAAHDEWVPKFKEFKVRNPRRVVFI
                     GTTNNKQILNDSTGNRRYLPMNSGDVDCAAIEKNRSQLWAEALHLFNENGVMWEAAETL
                     ARKVHDEFRVEDPWEDEIAELLKLDVDDCDPTAFVPTKALFSQVGLMIGHTSNKQHQRM
                     AAIMSRLGYEVGRKRINGALVRGYFRRTQAVV"
                     /name="VapE domain-containing protein"
     gene            complement(9767..10234)
                     /locus_tag="M0769_RS24490"
                     /old_locus_tag="M0769_24490"
     CDS             complement(9767..10234)
                     /locus_tag="M0769_RS24490"
                     /old_locus_tag="M0769_24490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_070559148.1"
                     /translation="MKKYTTEQKAQALRLLEQDGATAATVARTMGIPPRTVRDWAKART
                     DAPSNVLSIEEMRERAQRAVEATPQAAIRRLKNHFVQRQFELLQRHATDLQALRTASLQ
                     AMLEKDATMVKAISGLMTSLLKTQERERVIYEIKPGTEADIMREGMNRQQS"
                     /name="transposase"
     gene            complement(10433..10555)
                     /locus_tag="M0769_RS24695"
     CDS             complement(10433..10555)
                     /locus_tag="M0769_RS24695"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_256473491.1"
                     /translation="MKTYKKHRTKAIDRTVANQEYIHTMTEDGITVRYRLERWR"
                     /name="hypothetical protein"
     gene            complement(10797..10982)
                     /locus_tag="M0769_RS24495"
                     /old_locus_tag="M0769_24495"
     CDS             complement(10797..10982)
                     /locus_tag="M0769_RS24495"
                     /old_locus_tag="M0769_24495"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_119879885.1"
                     /translation="MINQAATPRYPGLSIEHGEIRQFGKPRHCAANRKGQYQTTLRDSA
                     GRYQTVSIISLYAWEA"
                     /name="hypothetical protein"
     gene            complement(11093..11680)
                     /locus_tag="M0769_RS24500"
                     /old_locus_tag="M0769_24500"
     CDS             complement(11093..11680)
                     /locus_tag="M0769_RS24500"
                     /old_locus_tag="M0769_24500"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_181654175.1"
                     /translation="MSKTYGYVRVSTDEQTSNSQRDAIIARFPTASILADDGISGTVPP
                     RERPALGILLNQILQEGDTLVIYAIDRIGRNARDALELVEELEKRKVTLVSIREGVDLS
                     SAVGQAVLGIMASFAQMERKLILERQAAGIEAAKSRGQKFGRPTKIGAKEAGEIVMRRA
                     MGVPIATIAKQLNLSRSAIYRHLEKVDAVAPE"
                     /name="recombinase family protein"
     gene            11940..12596
                     /locus_tag="M0769_RS24505"
                     /old_locus_tag="M0769_24505"
     CDS             11940..12596
                     /locus_tag="M0769_RS24505"
                     /old_locus_tag="M0769_24505"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_119879883.1"
                     /translation="MTEIKTECPDATWLRTTLAEMASDHFPVYDLPCLSVDPNSSAQLT
                     ALDDRRVAREMRQAAADVETRRIDAARVVEGLRTEAERLRGLIADGKTALRAGETVSPD
                     AGVAAFLLPDIEAELVAAEAAEADVARERDALLQDADRRDAAAAIGLFNLAHAVRVQRI
                     EQLLKLAMDEATLLAESDAGRSLYRTVINPDRRLNQLFSTQGAIEILRRNRGMEA"
                     /name="hypothetical protein"
ORIGIN
        1 ctgatgcgtc gcgtgctccg gcgttcgctg cggtgcgtgc tcaggtgcag caacaggcgt
       61 ctctctcccc tgctgcccgg ggtatcaatt gggacgagta aagccgatgc cgcgccacct
      121 gcagggtaag acgcggatgc cgctggcagg tggttgttac tctgctggcc accgtcttgc
      181 gctgatctcg ttgctgcctg ttctggaggc tcgtcctggt gacagggatg atggtgaacg
      241 atcgaagctg gcgctgtatc aggaatcgct gatcaaggcc gggcaaccgc acgcgttcgc
      301 gttacatgag ccgtcgctgt atcgctgggg ggcgtatctg aagagccgcc gcgcccggta
      361 tttacaggag acagacgcag gtgttgctgc ggcctcagac gcgcttgatc ggcatgcttt
      421 cgggcgtgct attggtgcgg tcctgggggc tgatgatgag tgagtttgcc gtctcgcgtc
      481 tggtcatccc tgaggtggtg atccctgacg gggttgttgc tgcgcgaaag gccaggggga
      541 tcgcgcaacg tgtcgcagct gctcgccgtg ctgctgaacg tcgtgctgcg cctacatggt
      601 gctgggtgag acggggcgcg ttatgcgccc tttttttcgc ccttcgcctg gcgtgccttg
      661 cgccggaatg ccttgatctc tgtcaggcgg gctttggcgt tttcggcctc ctccggggtt
      721 gctggttcgg tggggttgcc gtccaggtcg tagcgcatgc actggtcgat gaggctctgc
      781 tggtacgagc tcgtacccgt gacaatgccc aggatgcgac ggacggcttt gcggtctgcc
      841 tctggcggca tgttcagctc tgcgcctcgg gtcaggatct gctcgatgat gccctttgcc
      901 atggggcgac ggtcaactcc ccgggtcagc aagtcaggcc agtgctcttt caggaatgct
      961 ttggtcaccc ggttggccag cgcctttttg gacttgttgg cgatcgcgat gtcctgctgg
     1021 cggcggtcgt tctcctcctg tctgcggatc ccctcggcga cctgttcagg cgtctttttc
     1081 ggctttgccg ctgtgggctt ctgtgcgggc ttctgcgggg ctttcgcttt tggctgtacc
     1141 ttcgccttgc tggtagtccg ttcggctggc tgcggctcag atttcgcggc cttttccggc
     1201 gggacgtaga ccgtgcggtt gcgtcgcttg atggtcagga tcggacgggt ggtttcggtc
     1261 ataaatctct ctctggtcgg ttggtccgga tgtggtcggc aagggcgatc agggtggcgg
     1321 cgtcgccgcg gttccgatcg atgcgccgga tgatgtcgtt gatgtagtcc cagcgcccga
     1381 cggcggcaag gacggaaagg gcgaggcggt tgaaccggct ctctggtacc cggtcgaggg
     1441 tcagggggat gcgcttgcgg gggcgtcctg gaccgcgccc tgtccctggt cttttgagca
     1501 gctgcggctg ctccccctgc ggcaaaataa cggggatctt cgccaggctg cgcgtctcgt
     1561 tctgcgcgaa ctcgggcacc gcgatgtgct cgtctgtcca tgtcctgcct ccgggcctgg
     1621 ctgatgcgct ggagtcgcag gacgggcagg agacgacgat ctcccgtcgc ggcttgccct
     1681 tgaccggtgc cttgctgatc accaggggcg cggctttgca atcagcgcag accgacgatg
     1741 tccagaccgc gttgtctggc agggtggcgt tccggtactg gtgcgcggcg tcatgcggtg
     1801 tctggaagct gaccgtctga aacgtggggg cgacgctgag aggtgccgcc catggctggc
     1861 acctgggaca agcaagctcc acctctccgg cgtgcccgtc gccaccgttc cagcgcaggg
     1921 gctcgcggtg ttcggggcag tagtacacga aaccgtcgcg gcgtgtttgc ccgaccgggc
     1981 ctggtcggcg atcgtggaac cgtgcgcccg ggttggctcg catggcgcgg acgagcgcct
     2041 cgccctggtg gatgccgtac cagacctgcc agccatcgcc gatgccgatc aggaacggcg
     2101 agacgaacgg tggggtgcgg ttgactctcc cgcgtgcgtt ggctccgatg gtggcttcgc
     2161 gttcgcggtt ccagcaggag acgcacaggc tcgaaccggg cttattgggg gcgatcaggc
     2221 ggttgtcttt ccggctgcaa cgggggcaga gcttacgggt gtctgccggg gtggtcagtg
     2281 gtgcgtcagg gggctgttcg gtaccgaccc aaagcgcgag gccagtgggg caccggtggc
     2341 actcgaggtg ggggttttca atcgtgcgat cgaggtggct ctgctcataa atctgtcggc
     2401 atgcctcgac ccggagggag cacccgccgg ggcaggtgac gaacaaaacg ccgggcatga
     2461 tgtcggggaa ccgctccgca gcgcctacgc acttcagtgc ctcggttgac ggctgcgtcg
     2521 cttctgtcgt gtcgttatcg tcgttcactg ctccccctgc gtcgaaatgg tcgccagacc
     2581 tcgccagcac tggctgtgcg tcttttttag tatctcacaa aaaacgccac tttggcgtta
     2641 attgtgttaa gcttgtctta ctgattcctg acgagcagtt gggaaacatg aaaccggatc
     2701 gcccggtgcc gctttccctc ctccccaacg aggcggggtt gtaaacgagg atgcgcgctt
     2761 cgtccttggc aggtgcgagt aagcgagaag ggctgctgtc ctgccgctat gcggtaagtc
     2821 ctcaagggcg gctttatcga taatcgtcac tcccgccccc cgatgcccgt taagctgagg
     2881 ggggcaccta tctggtactc ccaatgaaaa aacgaatcta taccgctgac ctcgctgcgc
     2941 tgctgaacgt gtcgctgcgc acgctctccc gcctgagttc gcgggggatc gttccgactc
     3001 cggcagggcg ttcccttcgt ggcgggcatt actggctggc tggggatccc gtgctcaaac
     3061 gctttattgc cgaccagcgt cgtcccggga gggctcctta tggcgttcga taactctcac
     3121 ctgactgacg ctggcctgtt ctggcgtgcc gtctctgcgg ctggggctcc ggttgaccgc
     3181 gaggactggc tgcgcctctg ttctggcatc gcatgctggg catctctctg cggagaggac
     3241 gggcttgctg ctatcgaggc ctggtctacc cgccacaatc gcggatctgc gcatcaccgg
     3301 tcgcagtacc ggcacttcct gaagctgaaa ggcgtaacca atcccgaggc catggcgcaa
     3361 gtattcctgc ggttctcagg cgaggtgtac tggcggctcg actcaaatcg cacgccgccg
     3421 tcgactgaac aggttgctgc tcttgctgaa cggaaacgcc gatcgatcga ggcggtggcc
     3481 agacgcgaag atgccgaccg gcgcaaggcc gataaggctg cggctgatgc ggtcaggatg
     3541 ctggcgaaag gcgacggtaa tccggcctat accccgtact ggcgcaataa gaccacaatc
     3601 gccaggcgtg gtggcgtcag cctcccgccc ctccccgctg accttcgtgt gggtggcttg
     3661 ttccgctcgt tcgaaccgag ggcgtactgg tcgcgctgtc tgcttgttcc tgcctatcgc
     3721 ctgaccgatg atcgccagct ggttggcgac gcgctggagg ccatctgcgg cgtctgctct
     3781 gctggcggca aaaccggcga caagttctcc cctgcaggta cccaaaaggc gggacggttc
     3841 tatccgatgc ccggctttat ggacgtgctc gatacctcac cacatgcgcc ctactcgcct
     3901 ctgttcgtct gtgaggggtt cgccacggcg gttgccctgc atcatctgac gaagggcaag
     3961 gtggcagtct gcgcggcgtt ctcaattgcc gggttcggca gctgcgtggc gtcgctgcgc
     4021 cggttctggc cagatgttga tgtgatgctg gtaccggatc acggcgaagc gtaccgcctg
     4081 gcgcataaag cggcctatgg cgatgagcga gagccggtcc cgacaattcc aggcgcggcg
     4141 gttgtgtcgt tcgacggcct cgatcgcttc acgccccggg ataacctcga ctggttcgac
     4201 gtgctggttg aggaagggga agatcgggct cgtgagctgc tgcgctacgc ggttcgtcat
     4261 gcccggcttg agcggcgttg attggcgtgt ccgaatggtg cgcctttcgg gacatctttt
     4321 ttgccgtcgt ggcgtctggt ttagcgaacg actttcgaga cactaccgat gcttttttga
     4381 tgagacggac taaagcgacg caccggcgct gccgtcgtga cgctatcctg cgatcggttt
     4441 ctcacagcag cataaagccc ggcctcgtct tgatgtcggg ttttttttta gcccaaagga
     4501 gggcacatga aatcaaagac tgaactacgc gaggccgcga cggctcgctc tctggcggtg
     4561 atcgccactg aaatgtcgga gtggtcgctc gatgggttct cccacctcaa gctgccgaac
     4621 ttctccgctg gggaacgcca gcagacgctg tccgggtcgg tggtggttga tcgcccgccg
     4681 ttcgactacg agtgggctgg taccgagaaa tttaacactc tggccacccg tgcgctgcag
     4741 gtgaaacttc cggcatcgcg tgagcgtaac tacgcctggt tgtgcggcgt tgaacgggag
     4801 acgctggcga cggcgctcct cgtggagctg ttctccgtca ccggctgcgt ggcgttcgcc
     4861 gggctgggta aggttgccga tctggcgttc ctgacgctgg acgagagcga ggcggggcag
     4921 atcagggcgg cgatgctgca gtggctcgat ggggcggcgg cgtaggggct cgatcctcga
     4981 tcagctggag tgggaagggc gactgatcga ggaggccatg gcgacggaac tgcggggcga
     5041 cccggtgcgc tgcgaccacc ttgctgcggc gcggttatgg ctggagtgtg ttgaccgggc
     5101 gtctgatgac aggtggcggg cgttctgcct cgccagctat gaatggtgcg tattcgtcgc
     5161 agtagaactc tgaccggcgg cggcgttgag ccgtcgctcc tctgcgtgaa gcatcgatgt
     5221 taagagttat ttaacttctt tatgatcgct gcgccaagct gccgagtatt catttcattt
     5281 ccattgctat ttagcaatga tatttttaaa tcatgcgcaa catcacgtag aaatggcttc
     5341 acaatagtta caggcatgcc atttctttga actgaaatac cgccactaaa agtgcgactg
     5401 attgtataat cattccactg cacactttca gccgctcctg gcaagtcagg aacctcctgg
     5461 acagtgaaaa ccgcacgaac aatccctttt tccttccatc cacgcaaata cccactccaa
     5521 atatttgccc gaactgatga ttcttctgaa atcgcacgag cattccatac tccagtttca
     5581 caggaaccat cccttttaaa catttcgact ttggcctgtt ttgattttag taacgctttt
     5641 ttaaaatccg cttcaccttc aggattaaaa aaaagttcag ggcgtgtgtc atgatcgatt
     5701 tcaacaacat cgatcagacg gtttattacc tgttcgggag tttcaaatcc ctttgcttgt
     5761 gcagcaagac ggctatatgt agcgtcagat atcgtaatat ccatcaaagt acctctatag
     5821 atcaaaaatt aatcataaaa ttaatcataa ttgataaatt taatgatttc aagatctata
     5881 attacctgat atcgcaatat ggttaaccgc ctctaaagag ctataaaaac ctgtaaaccc
     5941 gctcctcgtg ccgcttggtt ataaacaaac cagttgaatc tacgtcaaca atagcctact
     6001 attgttgtac tgcaagagca ttcgcagggg gtattaacca gtaattaacc ctttgcgctg
     6061 ggcagtcagg aggctggtct cctgttggga agttatgttg cggcttccta gtaataacag
     6121 tgaatttgat agacagcaga aaaccgccga tgctgctaga tccgaaagga accaatgaag
     6181 ggaaatattt aggcgttatg gttcattctc attaaattga ggagaattgc ttaaatgggc
     6241 gtccttccta tcaccctcgc tgtatagctt tacatttggc ctccgttgca tggtatgcag
     6301 tcacgggggc caaattattt tcagagacat caactatcgc cattgcgaaa aagccagccc
     6361 cagcaacgtt aatgcgacga acagcattga gcacaatagt ctgctccgag taatctctgt
     6421 taacgtacac attgctctgc actgagaatg cagttgccaa aatctccgca cactcttgcc
     6481 aacttagctt atccttcgag ttacgagcag atagcacatg cctaaacatt gcccaagtga
     6541 tcggtattgc tggtgatgca ggtatgtccc agccgtttct ctgacaaaca actttcattt
     6601 gattgaactg cttcttagtc atcagatagg taaaacaagg cgtctcccaa acttcctgag
     6661 ggagaccttt atcatccatt tcttcagctt gctgtgtaaa agcatagaaa gcgtcacgga
     6721 tagcatcatc accgatagcc acttcttttc cttgtaaaat ttttatgttc gaattttatc
     6781 atatttagct atgtcactta ggactgctaa ttcagtagcc cactttcccg caacttatgg
     6841 cgatagtccc ggacacggct ggtgttccac tcctgtcccg tcgctgtccg gtgccctgct
     6901 gcgttcagtg ccgacgcgat ggcgggcaga gtcgcgttct ctgcggtcat gctgacaatg
     6961 agctggtcag cctcatcact gacggcctgg aagcgcggat cggctccccg ggggactatc
     7021 tgtcttgcag gttgttgtgc gctttgtggt gctgtttgtt gtgcggtttg tcgtgcaggt
     7081 ttaccgccga cggacagcgc cctgacttca gccctcagcg cttcgatggc agctaaaagt
     7141 ttggtgtcca tcccttccgg cttcgtgcca agtccggcgt tcagcgcctc tatgacagca
     7201 tccgtgcggg ttgctccggt tcgtgctgcg ttctgctcga tgcgttcgat caggctaacg
     7261 ggtaggcgaa cggtcagggt tttggttttc tctctcatgg ctgctgctct ccacgtcgtg
     7321 cgttttgtcg tgctgtttgt cttgcgtagt gtaatgcgtt ttgtcgtgcg cgtcgaattc
     7381 gacgtcgtgc agttgtcgtg cacagagggg cgacggcttt gcgtcgccgg ggtgccgttt
     7441 gtgtacatgg ataaatagca gaggcggcgc agagattgag ctgggcgtga tcacgacagc
     7501 gcgataagtg cgcggcgcta agtggcgtgg agggttggga tcgggtaagc tgatctggcg
     7561 tctgcggatc cggttgggga tcttcgggca caaaaaaagg cgacctgttg ggtggtcacc
     7621 tttttctgcc gggggagctg gtaactcctc cgattaattc gagtcacgtc ttagcgggac
     7681 gttctcgaag gaagaatagc acttgcgcag cgtactcctc aaactcaaag agaagtgtac
     7741 cacgtcgata agatcgcgca agtgtgaatt tccctcgtaa gcccccgcct gacgccgact
     7801 cctacgtttt taaaggagta attgcgtgtc acaaattgat gaaaattccg gcactgctgc
     7861 cgacaacgta acgccgatcg ccgctgccaa ggtgggacgc ccgtctctac aaaaaaaata
     7921 cccgactacc gcgtttcccg tggagctgga gatcaactac cggaaggggc gctactcgtc
     7981 ctctcttgag aacgttgtcg ccgctatgcg ctgccattcc gtcaccggca tccagctcgc
     8041 tttcgatgag ttctttgcgg aggagggggc gttcgtgaac gggcaatggt acccgctgtc
     8101 cgacaaccta gttaccgagc tgcgtctgcg cctcgctgct gccgacttcg agaaggtgac
     8161 caaggacaat gcccgcgacg ccatgttcta cgtctgcgat gagaacaaat tcgacagcgc
     8221 ccagcgttgg ctgacggctc aggaatggga cggcaaacct cgcgtcagtt acttcttcga
     8281 gcggtgcttc aaatgccaag gggatcccga gtacctggcg gcggtcagcc tctacacctg
     8341 gacggcgctt gccgggcgcg taatgaatcc cggaatcaag gccgacatgg ttccgctgct
     8401 gtacgggcga cagggtgccc gcaaatcgac gggcgttcgc cttatgtcgc cattccctga
     8461 ctcctactcc agcttcaacc tgaacgacaa ggacgactcc ctagtgcgcc gtatgcgtgg
     8521 gtgcctgatt ggcgagctgg aggagctgcg cggcctccac gggcgcgact ccgaatcggt
     8581 gaaagcgttc ttctccgctg cccacgacga gtgggtgccg aaatttaaag agttcaaggt
     8641 gcgaaacccg cgccgcgtgg tgttcatcgg taccacgaac aacaagcaga tcctcaatga
     8701 ctcgacgggg aaccgccgct atttgcccat gaattccggg gacgtggact gcgcggccat
     8761 cgagaaaaac cgctcgcagc tgtgggctga ggcgctccac ctgttcaatg agaatggggt
     8821 gatgtgggag gccgctgaaa cgctggcgcg taaggtgcat gacgagttcc gcgtcgagga
     8881 tccgtgggaa gacgagatcg ccgagctgct caagctggac gttgacgact gcgacccgac
     8941 tgctttcgtg ccgacgaagg ccttgttctc tcaggtaggg ctcatgattg gccataccag
     9001 caacaagcag catcagcgca tggcggctat catgtcccga ttgggctatg aggttggcag
     9061 gaagcgcata aacggtgcgt tagttcgtgg ctacttccgt cgaacgcaag ctgtcgtgta
     9121 agggttactt ttccgtaaga ggggagctat atgctcccct tttttattgt tccacccggg
     9181 tggatcacgg gtggatcatc acgccgtttt tgtaccacct gttccacccg ggtgttccac
     9241 ccgttccacc cgtgttccgc ccgtggatgg aacagctaag gcctcgtgtt taaaggcttt
     9301 cattgttgtg ttccacccgt tccaccctat tttctaatta aaaacaaaaa atgaataaat
     9361 tgggtaaata gggggtaaat gctcactaat tgaacaatta gttgtttttt ttactattta
     9421 tgaatctatg ccggatcggg tggaacgggt ggatcatctt ggaatcgacg cgccgcagct
     9481 gcaccctgcc ggatgcactt tgcttgtctt attagatcgg tggatgggtt cgtcgtgccg
     9541 ggatggtcgg tggtagtggt tctgaggggg atcggaggtt tgggtggcgc tgagggcacg
     9601 aggctttcgc ctcgcgctgg gtggtcgtct gttggttctg cgtgagggct tgccctcgcg
     9661 cacgcgagcg gtaacaactc gttaacgtgc ggatccgcgt atttcgcggg ctcccgggtt
     9721 ttctgcggcg gtaaagcggc ggtaaaaggc ggttttcccg ccgttttcag ctctgttggc
     9781 gattcatgcc ctcgcgcatg atgtcggcct cggtgcctgg cttgatctcg tagatgacgc
     9841 gctcgcgttc ctgcgtcttg agtagggatg tcataaggcc gctgatcgct ttgaccatgg
     9901 tggcgtcctt ctccagcatg gcctgtagcg aagctgtgcg gagggcttgc aggtcggtgg
     9961 cgtggcgctg tagcagctca aactggcgct gtacaaagtg gttttttagg cgtcggatcg
    10021 ctgcctgtgg ggtggcctcg acggctcgct gtgcgcgttc gcgcatctcc tcgatgctga
    10081 ggacgttgct gggtgcgtcg gtgcgggctt tcgcccaatc tctgacggtg cggggtggga
    10141 tccccatggt gcgtgcgacg gtggctgcgg ttgcgccgtc ctgctccagt agtctgagcg
    10201 cctgggcttt ctgctcggtg gtgtatttct tcatggtgtc ctgctccggt tggtttctct
    10261 cctgtattga aacgtcacgc gctgaaaatg gcgggggttt ttggggggta cacggcgttt
    10321 gtgcgtgcgt ggtcgtgcgg ttgggtattt ttgtggctga ggcgcttaga atggctctgg
    10381 tgcgttctgg gtaagggagg aggcgcagca gtcgctgcgc tcctagctgg ggttagcgcc
    10441 agcgttcgag gcggtacctg acggtgatcc cgtcctccgt catggtatgg atgtactcct
    10501 ggtttgccac ggtgcggtcg atggccttgg tgcggtgctt tttgtacgtt ttcatcctgg
    10561 tttcttcgga gaagttctcg ccggtaacca tctcgtccca tgactcatat gactttgctc
    10621 cgggaaccat gattaccttc catctctgga acctacgggg aatgttttca aatgccatcg
    10681 ttctctcctt atgattctgg tttctggggg cgctcccgcg cccctgtaag tggttcgtaa
    10741 tggcgtaagg cttttgttat tgcttgctgc ttacacctgt aaggctttcg ttatggtcat
    10801 gcttcccagg cgtaaaggct gatgatgctg actgtctggt accgaccggc gctgtcgcgg
    10861 agggtggtct ggtactggcc tttgcggttg gcggcgcaat ggcggggctt gccgaactgg
    10921 cggatctcgc cgtgttcaat gctcaggccg ggatagcgcg gtgttgctgc ttggtttatc
    10981 atggttctgt tccttgttgg gaatggtgcc cgggtctgct cctcatgcgt tccccgggct
    11041 gggttgcggc ggtgtggaat ggttggagcc acctacaccg ctgcgctctc tcttactccg
    11101 gcgctaccgc gtcgaccttc tcaaggtggc ggtaaatcgc gctcctgctc agatttaact
    11161 gcttggctat ggtggctatt ggtaccccca tggcgcggcg catgactatc tcccccgctt
    11221 ctttcgcgcc gatcttggtc gggcgtccga acttctggcc acggctcttt gcggcctcga
    11281 tccctgccgc ctggcgttcc agtatgagct tgcgctccat ctgcgcgaaa ctcgccatga
    11341 tgccgaggac ggcctgtccg acggcgctgc tcaaatcgac accctcgcgg atgctgacca
    11401 gggttacttt tcgtttctcc agctcctcga ccagttcgag ggcgtcccgg gcgttcctgc
    11461 cgatgcggtc gatcgcgtag atgaccagcg tgtcgccctc ctgcaggatc tggttcagga
    11521 ggatgccgag ggctggacgc tcgcgtggtg gtaccgtccc gctgatcccg tcgtcggcga
    11581 ggatggaggc tgttgggaac cgggcgatga tggcgtcgcg ctggctgttg ctggtttgtt
    11641 cgtcggttga tacgcgaacg taaccgtatg ttttgctcac ttttttctct cctctgaccg
    11701 atacgtctct ttcgacacaa catgccacaa ctgtaccgaa agtctatcgc taaagtgtct
    11761 atttcgtcgc tttgtcgggt gtcccggaat gtaggggatt agcgacggcc tccaggcggc
    11821 tctccgcgct gggcgagagc cctgcgccct ctgcgttgat gtctgcgcgg tgtcgtgacg
    11881 gcatgctgtc tgagcggcgt ttgctgccgc tttgctgata acttgctgag aaccaaacca
    11941 tgaccgaaat caaaaccgaa tgcccggacg cgacctggct gcgtaccacg cttgctgaaa
    12001 tggcgagtga ccattttccc gtttatgacc ttccttgcct gtcggtggat cctaactcct
    12061 ccgctcaact taccgcgctt gacgatcgcc gggtggcgcg tgaaatgcgt caggcagcag
    12121 ccgacgtgga aacgcgacgc attgacgccg ctcgcgtggt ggaagggctc agaacggaag
    12181 ctgaacgcct gcgcgggctg attgccgacg gcaagacggc gctgcgtgct ggcgagactg
    12241 tgagccccga tgcgggcgtc gctgcgttcc tgctgccgga tatcgaggcc gagctggttg
    12301 ccgccgaggc tgctgaggct gatgtggccc gggagcgtga cgcgctgctg caggatgctg
    12361 accgccgtga tgcggcggct gcgatcggcc tgttcaacct ggcgcacgcc gtgcgcgtac
    12421 agcgtattga gcagctgctg aagctggcga tggatgaggc gacgctgctt gccgagtccg
    12481 acgcggggcg cagtttgtac cgtactgtta tcaatcctga tcgccgcctg aaccagctgt
    12541 tctccacgca gggtgcgatc gaaattctgc gccgcaatcg cggcatggag gcgtaaatgt
    12601 ctgcattttc gatgctgtgt gaccggcttc gccatgaggc gacgctggca aaatctaccg
    12661 gggccggcga cgcgctggcg caggtgctgg agacgggcgg tgctgcgcag acgttcgcgc
    12721 cgatcggggc tccggttgcc tctcctacgg agtcggttca ccgggcgcgt gctcagtcca
    12781 tccaggatgc ggcgttcctg actgagatga aacaggcggg cgtggctcct gccgatctgg
    12841 tgatgcttga cggcgtgctg gcctcctctc gcggactcc
//
