LOCUS       NZ_CP095794             2313 bp    DNA     circular CON 21-APR-2024
DEFINITION  Escherichia fergusonii strain EF21JD053 plasmid pJD053-2K, complete
            sequence.
ACCESSION   NZ_CP095794
VERSION     NZ_CP095794.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27582503
            Assembly: GCF_023094055.1
KEYWORDS    RefSeq.
SOURCE      Escherichia fergusonii
  ORGANISM  Escherichia fergusonii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Tang,B., Guan,C., Lin,H., Liu,C., Yang,H., Zhao,G. and Yue,M.
  TITLE     Emergence of co-existence of mcr-1 and blaNDM-5 in Escherichia
            fergusonii
  JOURNAL   Int J Antimicrob Agents 61 (3), 106742 (2023)
   PUBMED   36736926
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Tang,B., Guan,C., Lin,J., Yang,H. and Liu,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023094055.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 14:02:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,120
            CDSs (total)                      :: 5,003
            Genes (coding)                    :: 4,813
            CDSs (with protein)               :: 4,813
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 60 of 190
            Pseudo Genes (incomplete)         :: 141 of 190
            Pseudo Genes (internal stop)      :: 31 of 190
            Pseudo Genes (multiple problems)  :: 37 of 190
            Pseudo Genes (short protein)      :: 2 of 190
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095794.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Escherichia fergusonii"
                     /mol_type="genomic DNA"
                     /strain="EF21JD053"
                     /isolation_source="cecal contents"
                     /db_xref="taxon:564"
                     /plasmid="pJD053-2K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-12"
     gene            complement(join(2065..2313,1..177))
                     /gene="mobA"
                     /locus_tag="MYB88_RS25505"
                     /old_locus_tag="MYB88_25500"
     CDS             complement(join(2065..2313,1..177))
                     /gene="mobA"
                     /locus_tag="MYB88_RS25505"
                     /old_locus_tag="MYB88_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_024257935.1"
                     /translation="MAKNKRSGSEVRQRTKVITLRVNNRLASEIRRRAKNEGLTISEYI
                     RTASLNNEIKQRVPSRYLYELIRLGRMQKKLFDKGKRPKDKEYLEVMHKIILLCDEMKI
                     VTKRISDIYNEMDLIKDEIKIIKRLHKNKYPGSDLFK"
                     /name="mobA"
     gene            1534..2031
                     /locus_tag="MYB88_RS25510"
                     /old_locus_tag="MYB88_25505"
     CDS             1534..2031
                     /locus_tag="MYB88_RS25510"
                     /old_locus_tag="MYB88_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027759.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_023156898.1"
                     /translation="MNITERTDSKKKVLRQNITEVIDRETGNVTQEITDITVQFPQEPA
                     YVKIYIDNLCAVTKAPDSLKDVLFLILRKLDYDGYIALSTRYRKEICKLLGIKDGTLRN
                     RLYSLSKMGIIASCGGNEYQANPNLFARGEWKKIIEQRREFEMRIKYSPNGETIITTEE
                     IK"
                     /name="replication/maintenance protein RepL"
     Typing          complement(925..1183)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.842"
                     /coverage="100.0"
                     /evalue="1.48e-75"
ORIGIN
        1 aggtacgcgc tgttttattt cattatttaa acttgccgtc ctgatatatt ctgaaattgt
       61 cagtccttcg ttcttcgctc gccgtctgat ttcgcttgca agtctgttat ttactctaag
      121 ggtgataacc ttagttctct gccgtacttc tgaaccacta cgtttatttt tagccatcgc
      181 aatgactctc cattttgcac gtttggggtg tcggggcttg ccctgacagg aggtattctc
      241 atgcaagcga atgtattcgc ggtatgacga atacacatcc tgtcccattt tccgctgcaa
      301 cttttcaacg aaaataaaat accgcgtcgt aacctggtat ttctgataca aaaaaatgat
      361 gcttcattca atattattta cacagatttt aaaaattatt gcttgttatt taataaataa
      421 caccgaatta attgatgtct ttgttgtctg tcgtttgtgg attattagag gaataaagat
      481 tagatcttct tggttatata atccattgcc tgttggtaat gcgggattcg tccagagagc
      541 aggaggcaaa agagcaagat ggtagcatat gcataaaata taactatgtg agttattgat
      601 atcgcgttta tatattaaaa atataactta acgtcgctcc taatctttat ttcatcctgg
      661 caatgggagc attaatcccc gtgcagtgct ccgccagctg ctgacaacct cgacatataa
      721 ccatcgatac gatagaacat cagggcgcgt ttccggcaag aactaaccat ggggatggtt
      781 tgcgtgccaa cgaacgccgg aaacgcgctt caaacctacc gagtcgccac ggttccggca
      841 gcgccttgag cgcaaccgga aaccgtctgg ggagaacccc agacccccac cgctgcaaca
      901 tcgacggtct atgaccaccc accaaccgag tagctgccca tcccgtgcaa atgggctttg
      961 cagggggctg gcgcggttcc tggcgacctg aaagcccctg gttaggggta tcggcgcgcc
     1021 tggcctcctg aaaccctctg taagcgtttt tcgcgccttc catgccctga cgggcattta
     1081 gaccccgttt cgtgcgtctg ggaggctctg agcgcgttta acagatgacc agcgactcga
     1141 tcgcctgaga tcaaatcggg atcatcaacg atatgcgaga ggtggcaccg ctcgccgcag
     1201 ccccagcgac cgagccgcca ggctcgcgag tgggcgagga agcggaagag aaccggaagc
     1261 tacattgagc acgcacgctc aatattaaaa ttgtcacttc gtaaatagag attatgcctc
     1321 gtgcttgtgg atacatttaa aaatataaga tatccgaatt aggctacata cgggctgatc
     1381 gccaatgttg taacgtgtag aagatacacc tgtaacgtgt acaggataca cctgtaacgt
     1441 gtgggcggta ttcgcgtaat ctatattgag tgacattttg ttttttgggt attatattgt
     1501 ttgtcacctt tataaagtga caaaggcgat aaaatgaaca ttactgaaag aacggatagc
     1561 aagaaaaaag ttttaagaca aaatattact gaggttatag atagagaaac tggaaacgta
     1621 acacaagaaa taaccgatat aacagttcaa tttccacaag aacctgcgta tgtgaaaatt
     1681 tatattgata atttatgtgc tgtcacaaaa gcacctgatt ctctaaaaga tgttttattt
     1741 ttaattctta ggaaattgga ttatgacgga tatattgcat tatcaactag ataccgtaag
     1801 gagatctgta aattattggg aataaaagac ggaacattac gtaacagatt gtattcatta
     1861 tcaaaaatgg ggattattgc tagttgtggc ggtaatgaat atcaggcgaa ccctaattta
     1921 tttgctcgtg gtgaatggaa aaaaataatt gaacagcgtc gcgagtttga aatgagaatt
     1981 aaatattcac ctaacggtga aactattata acaaccgaag aaataaaata atgcgcacct
     2041 aaataaggtc tttttaaaaa gaccttattt gaataaatcc gaacctggat atttattttt
     2101 atgtagcctt tttattattt ttatttcatc ctttattaaa tccatttcat tatatatatc
     2161 agaaattcgc ttcgttacta ttttcatttc atcgcaaagt aaaattattt tatgcatcac
     2221 ctcgaggtac tctttatcct tcggccgctt tcccttatca aataattttt tctgcatccg
     2281 accaagtcgg attaattcat ataaatacct gga
//
