LOCUS       NZ_CP095536             6797 bp    DNA     linear   CON 20-JAN-2024
DEFINITION  Escherichia coli strain GN02461 plasmid p2461-4.
ACCESSION   NZ_CP095536
VERSION     NZ_CP095536.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27361468
            Assembly: GCF_026651245.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Bethke,J.H., Ma,H.R., Tsoi,R., Cheng,L., Xiao,M. and You,L.
  TITLE     Vertical and horizontal gene transfer tradeoffs direct plasmid
            fitness
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   Bethke,J.H., Ma,H.R., Tsoi,R., Cheng,L., Xiao,M. and You,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2022) Molecular Genetics and Microbiology, Duke
            University, CIEMAS Building 101 Science Drive, Durham, NC 27705, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 6.0.0.47841
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 304x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/20/2024 04:54:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,079
            CDSs (total)                      :: 4,961
            Genes (coding)                    :: 4,796
            CDSs (with protein)               :: 4,796
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 66 of 165
            Pseudo Genes (incomplete)         :: 114 of 165
            Pseudo Genes (internal stop)      :: 38 of 165
            Pseudo Genes (multiple problems)  :: 48 of 165
            Pseudo Genes (short protein)      :: 4 of 165
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095536.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GN02461"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2461-4"
                     /geo_loc_name="USA"
                     /lat_lon="36 N 78.9 W"
                     /collection_date="2006"
                     /collected_by="Vance G. Fowler Jr."
                     /genotype="68"
     gene            complement(130..363)
                     /locus_tag="MW400_RS25230"
                     /old_locus_tag="MW400_25220"
     CDS             complement(130..363)
                     /locus_tag="MW400_RS25230"
                     /old_locus_tag="MW400_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_059331885.1"
                     /translation="MTELERHLLNALEHLQQDYTQRLKDWESAFTDLQHMFERTRQENG
                     QLREQCDNLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(370..930)
                     /locus_tag="MW400_RS25235"
                     /old_locus_tag="MW400_25225"
     CDS             complement(370..930)
                     /locus_tag="MW400_RS25235"
                     /old_locus_tag="MW400_25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_137498897.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISGHTTSVKQQLDTLSTTVSTQFSTTEAELSRQQKNLLWQVIKGRILFPSLTA
                     LSVTGGIFLGCWGLIQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<965..1618)
                     /locus_tag="MW400_RS25240"
                     /old_locus_tag="MW400_25230"
                     /pseudo=""
     CDS             complement(<965..1618)
                     /locus_tag="MW400_RS25240"
                     /old_locus_tag="MW400_25230"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1608..1931)
                     /locus_tag="MW400_RS25245"
                     /old_locus_tag="MW400_25235"
     CDS             complement(1608..1931)
                     /locus_tag="MW400_RS25245"
                     /old_locus_tag="MW400_25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_079774495.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1995..2186
                     /locus_tag="MW400_RS25250"
                     /old_locus_tag="MW400_25240"
     CDS             1995..2186
                     /locus_tag="MW400_RS25250"
                     /old_locus_tag="MW400_25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_064767211.1"
                     /translation="MTKQEKTALNMARFIRNQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2850..3011
                     /locus_tag="MW400_RS25425"
     CDS             2850..3011
                     /locus_tag="MW400_RS25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_227474774.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLSPLAAATGNRISRARFI
                     QMLTEFLK"
                     /name="replication initiation protein"
     gene            complement(3588..4610)
                     /locus_tag="MW400_RS25255"
                     /old_locus_tag="MW400_25245"
     CDS             complement(3588..4610)
                     /locus_tag="MW400_RS25255"
                     /old_locus_tag="MW400_25245"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]; GO:0043565 - sequence-specific DNA
                     binding [Evidence IEA]; GO:0000976 - transcription
                     cis-regulatory region binding [Evidence IEA]"
                     /GO_process="GO:0006351 - DNA-templated transcription
                     [Evidence IEA]; GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]; GO:0006355 -
                     regulation of DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_059331887.1"
                     /translation="MNKSSSISDVAQLAKVGKTSVSRYLNGEHHLLSEKLKERIKNAIE
                     VLDYTPNPLARSLKNKRSKLIGLLLADINNPYSVRIINSVEKYCWENGFVPIVFNTDNS
                     FEKESKALDILNRYQVEGVVINTVNSSKPFGEYRFPVVLIDRKIPGKLFDMVGLDNYHA
                     VNTAVQHLVNEGFDDILFISEPVMGVHTRLERQRAFYNSSRCFHDIQYHAQEIGCLDID
                     GIEKILLTFCDKRINAKRKAIISANGALTISLIKVINYLNIRIGVDVGFLGVDDVEWSE
                     IIGGGITIIKQPTDLIGSAAMELLHSRIDTPDIPSRDAIFHGDLIIRQSTINLSRFQSK
                     "
                     /name="LacI family DNA-binding transcriptional regulator"
     gene            4809..5777
                     /gene="kdgT"
                     /locus_tag="MW400_RS25260"
                     /old_locus_tag="MW400_25250"
     CDS             4809..5777
                     /gene="kdgT"
                     /locus_tag="MW400_RS25260"
                     /old_locus_tag="MW400_25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006810427.1"
                     /GO_function="GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA]"
                     /GO_process="GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="WP_059331886.1"
                     /translation="MKIKATIEKIPGGLMLVPLLLGAILNTFAPETGNYFGGFTKGLIG
                     GTVPILAVWFFCIGASISLKASGIVLKKSGTLVLTKIAVAWLVAFVCTKFIPSDGIQTG
                     FFAGLSVLAIVSAMDMTNGGLYASLMNQYGSKEEAGAFILTSLESGPLMSMIILGTAGL
                     ASFEPQHFIGAVLPFLIGFLLGNLDEELKEFFTKANQVLIPFFGFALGNAINLGVILDT
                     GLLGIILGLAVIVITGIPLILADRIIGGGNGTAGVAASSTAGAAVANPVLIAQINPIFE
                     PVAASATALVATCVVVTAILVPIITALYAKHISPKMKTKTL"
                     /name="kdgT"
     gene            complement(6546..>6797)
                     /locus_tag="MW400_RS25265"
                     /old_locus_tag="MW400_25255"
     CDS             complement(6546..>6797)
                     /locus_tag="MW400_RS25265"
                     /old_locus_tag="MW400_25255"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_268100685.1"
                     /translation="RMKKFGPVMDHSRGMRILRDVTESICQITVKKTRLSPAKMGNIML
                     CSCGVHITTNNLRIVFSPPCSKGFFFERASDTNPVSHP"
                     /name="hypothetical protein"
     Typing          complement(2268..2352)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.209"
                     /coverage="99.0"
                     /evalue="2.41e-18"
     Typing          2363..2922
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.296"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(54..1618)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.564"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1615..1961)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.207"
                     /coverage="98.0"
                     /evalue="6.15e-59"
ORIGIN
        1 tactcagact tctctgttgg agacccccgt tagggggagt tttttagagc tccattgacg
       61 gaccactccg actccgcgcc acgcgttcct gtatcttata tctgtaccgg gcacgatcct
      121 gctcatccct tatctgctgt tcgtgataaa taaccgactc aagcggtcca cctgcccggc
      181 taatctctgc acctgctgac tcaagttgtc gcactgttcc ctcagttgcc cgttctcctg
      241 tcgtgtccgc tcgaacatat gctgcaaatc cgtgaaggcg ctctcccagt ctttcagtcg
      301 ctgcgtatag tcctgttgca aatgctctaa ggcgttcagc aaatgccttt ccagttccgt
      361 cattctcgtt tactccctga ccagcctcac ggcgttcttg tcgcccaccg tccaggtgtt
      421 ttcccctttc accccgtccg gcaacaccag aaatttcccg ttctcgccat tgacgaacgt
      481 caccccccag gtttttgcct cgagtttcgc cagcgtgttt tcctgctctc tgatggtcag
      541 gatatttttc gcaatcctgc tttcctgcca ctgaatcagc ccccagcacc ccagaaaaat
      601 gccgcccgtg acgctgagcg cggtcagcga cggaaacagt atccgtcctt tgatcacctg
      661 ccagagcagg tttttctgct gccgcgacag ttcggcctct gtggtgctga actgcgtgct
      721 cacggtggtg ctcagcgtat ccagctgctg tttcacggat gtcgtatgtc cgctgatagc
      781 gtctctgatt ttctgcctgt ttaagctcag tgccttgtct acagactctt cgagcttcct
      841 gaactcgctg ttcagcatgt tctcggtaga agcggctcgc tctttcagtt tcttctcgaa
      901 gtctgtcccc atttgtaaaa gattgctcat acagcgcccc ctttagcctg agattccgcc
      961 ccccctccgg gtcggcaata ctgatgctgc ttctggttgt tctcaccacc tcaaaacctg
     1021 ccgctgtaag cgcctctgtg acatcctgac gcgtttttat tgctccggca tggtaaagag
     1081 cctcaagacc ccgtgtaatc gcttctgcgg cctcctgttt cgcttttggc agattatttg
     1141 gcgtgacaag cgtccgtctg ttctccggtg cgttcggatc acgcagcccg taatgatgat
     1201 taaccagcgt ctgccaggca tttatcctcg gcctgtccgc acggtcgtag tacggctgga
     1261 ggcgcttacc gctctgtagc tccatgttcg ggatgacaaa attcagttcg agccgcccct
     1321 tatcctggtg ttccacccag agaatgctgt actggtcttt ttcaagaccg ggcataagaa
     1381 cacgctcaaa gctcgtcatc actctttcgc gttcacccgg cggtaactcc ttctccgcga
     1441 acgacagaac gcctgaagtg tatttcttcg aaaatggcgt ggaatcaatg agttcccgca
     1501 cttcttccgg gttaccccgg agcaccgttg cgccttcgcg gttacgctcc cgccccagca
     1561 ggtaatcgac cgggccactt ccaccgcctt tccccctggc atgaaattta acgatcatcc
     1621 cgtaccccct gttccctgac agcctgccgc aactggcgca attcaccctc gatggccatc
     1681 agtgcggcca ccacatgaac ccggtcaatc gaagaccact ggccgctgtt caccttccgg
     1741 gctgtctggt tcaggttgtt cccgatggca gccagatgcc gcagcaatgg cggtgccagt
     1801 gtcggcagtt ttcctgtgcg tgcaaccggc tcccccagac agacccgccg catccattcc
     1861 gccagtcgtt taccctcgca gcgttccagt aaccgggcgt gctcctcatc ggtaacccgt
     1921 atcgtgagca tcttctcgcg tttcatcggt atcattcccc ctgacaaatc ccccatacaa
     1981 ggagcattag gaccatgacc aaacaggaaa aaacagccct taacatggct cgctttatca
     2041 gaaaccagac gctgaccctg ctggaaaaac tcaacgagct ggacgcggac gagcaggcag
     2101 acatctgcga atctcttcac gaccacgccg acgagctcta ccgcagttgc ctcgcacgct
     2161 tcggcgatga cggtgaaagc aactgaaaca tgcagctccc agagacgttc acagcttgcc
     2221 tgtgagcgga tgccgggagc agacaagccc gccagggcgc gtcagtgcgg gtgtcggggc
     2281 gcagccctga cccagtcaca gagcgatagc gatgtgtata ctggcttatc tcattttgca
     2341 ttgctgcgga ttgtatgaga agtgcgccat gtccggtgtg aaatgccgca cagatgcgta
     2401 aggagaaaat gcacatccgg gcacttttcc gcttcctcgc tcactgactc gctacgctcg
     2461 gtcgttcggc tgcggcgagc ggtatcagct cactcaaaga cggtgatgcg gttatccaca
     2521 gaatcagggg ataacgccgg aaagaacatg tgagcaacaa gacaaagcca aggaaaaagg
     2581 ccgcgccgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaaccgac
     2641 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2701 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2761 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgttggtat ctcggctcgg
     2821 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2881 gcgccttatc cggtaactat cgtcttgaat ccaacccggt aagacacgac ttatcgccac
     2941 tggcagcagc cactggtaat cggattagca gagcgagatt tatccagatg cttacagagt
     3001 tcttgaagtg atagcccaac tacggctaca ctggaaggac agaatttggt atctgcgctc
     3061 tgctaaagcc agttacctcg gttaagcagt ccccaactga cttaaccttc gactaaaccg
     3121 cctccccagg cggttttttc gtttacaggc agcagattac gcgcagaaaa aaaggatcac
     3181 aagaggatct cctgatcttt tttcaccaaa ccaccatcta tgtcagcgac aaagataatg
     3241 ttacccctcc ccataaccga gtagcaccta taaccaccaa cataacgatg tgtattgcgt
     3301 agcaaattgt attaattacc tttttacctc cacttaacag tgggggagtc tatcttcaat
     3361 ggagaaaatg acgcaatctc ttatgtatcc ggttagatgc cttaataacc agtaacagtt
     3421 ctattgaaca actcaacaac atcacacgaa ctaacattca cacatagata taaatcacga
     3481 ggagagataa tcggttatca gctatcgttc tctcctccaa agcatctaat caagattatc
     3541 taccgttcag agataagcct catcaattta ttaaggcatt agcatattta cttagattga
     3601 aacctactta agtttatagt agattgccga attatcaaat cgccatggaa tatagcatcc
     3661 cgtgaaggaa tatcaggagt atctattctg gagtgcagca attccatcgc tgcactccct
     3721 ataagatctg ttggttgctt tattatagta attcccccgc caataatttc agaccattca
     3781 acatcatcaa ctcccagaaa accgacatca acacctattc ttatattaag atagttaatt
     3841 acctttatta gacttatagt taatgcgccg tttgctgata taatagcttt tctttttgcg
     3901 tttattcttt tatcacaaaa ggtaagtaga attttctcaa taccatcaat atccaaacac
     3961 cctatttctt gcgcatgata ttgaatatcg tgaaaacatc ttgatgaatt ataaaatgca
     4021 cgttgccgct caagccttgt atgcactccc ataactggct cactaataaa tagtatatca
     4081 tcaaaaccct cgttcacaag atgttgaacg gctgtattta cggcatgata attatcaagc
     4141 cctaccatat cgaataattt tcctggtatt tttctatcaa ttaagaccac cggaaagcga
     4201 tactcaccaa atggttttga agaattgacg gtattaatta caacgccctc tacctgatat
     4261 ctatttaata tatcaagagc tttactttct ttttcaaaac tattgtctgt attaaagaca
     4321 ataggaacaa agccattttc ccagcaatat ttttcaacac tattaatgat tcttacagaa
     4381 tatgggttat ttatatcagc cagtaaaagc cctataagtt tacttcgttt attctttagg
     4441 cttcttgcta agggatttgg agtataatca agaacctcta tcgcattttt tatacgctcc
     4501 ttgagttttt ctgataacaa atgatgttcg ccattgagat atcgagacac acttgtcttg
     4561 ccaacttttg ctagttgggc aacatcagag atagaactac ttttattcat ttttgatacc
     4621 ctatgttcag gctggcatac cacttattcc aatttgttat acacatcatg acaatcactc
     4681 acatccattg gaatatatga ttttgcggat tacttttttt ttgacacgat tcacataact
     4741 atattttcca tgatgcactg gaatcggttc catgatgaac taaagaaaac aattcaaagg
     4801 aatgcattat gaaaatcaaa gcaacaatag aaaaaatccc aggtggttta atgcttgtac
     4861 cattactgct tggtgcgata ttgaacacct ttgccccaga gacaggcaat tattttggcg
     4921 gtttcactaa aggcttaata ggaggcacag taccaatatt agctgtatgg tttttttgta
     4981 tcggtgcctc aataagccta aaagcttccg gtattgttct aaaaaaatca ggtacattgg
     5041 ttttgacaaa aattgcagta gcctggttag ttgcgttcgt ttgtaccaaa ttcattccaa
     5101 gtgatggtat ccagaccggt tttttcgcag gtctgtctgt tttagctatt gtatccgcca
     5161 tggatatgac gaatggtggc ctatatgcca gcctaatgaa tcaatatgga agtaaagaag
     5221 aagctggagc atttatctta acatctttgg aatcagggcc attgatgtcg atgattattc
     5281 tgggaacagc aggacttgca tcctttgaac ctcaacattt cattggtgca gtattaccat
     5341 ttctcattgg ctttttactc ggtaacttag atgaagaatt gaaagaattc ttcacgaaag
     5401 ccaaccaagt cctgatccct ttctttggat tcgcgttagg taatgcgatt aatcttggcg
     5461 tgattttaga tacaggatta ttaggtataa ttcttggact ggcggttatt gtcattaccg
     5521 gaataccatt aatattggcg gatcgtatta ttggaggggg gaatggcact gctggtgtgg
     5581 cagcctcgtc cacagcaggt gctgcggtag caaacccagt tttaattgca cagattaatc
     5641 caatatttga gcctgttgca gcatcagcta cagcattagt ggcaacatgt gttgttgtta
     5701 ccgctatttt agtgccaatt attacggcct tatatgcaaa acacatatca ccaaagatga
     5761 aaactaaaac tctttagttg cagctgaaca tttcgttagg acaaaaagcg tcagcctggc
     5821 gctttttgtc tggcttataa gagtactgat tccagacacg ataaaatcct caccgccccc
     5881 tacagcaaag acttatttaa cataaatata ctttagacgg agatgatgga gccgcagccc
     5941 ctcccggttt tcttttgctt tagcctgtgc ttctgcggca gcttttgccc gggctgtctg
     6001 ttctgcctgc gttcaaacgg acaaaaaaaa gcccatctgg aagaacgggc aaaaagtaat
     6061 aatgtgattg tttaactacg ctactctgcg tcctgaattt gccgcaattc aacaaaaaac
     6121 acaacccatc catatcgcca gtaaccgaga aaccttgcca ttttgacccg ttcgacggct
     6181 ggaacgtgcg aaactgatcg tcattcagtt tcgcgcataa ctatgcatga ggttaaaatt
     6241 taccaggcgc gatcgcggca acttttcggg tggtttgttg ctggttttac ctgtctgttg
     6301 ccgtgatcgc accgaacgtg ttttagcggt gcgcgcaacg ggagttatgg taaatggatc
     6361 gggtttgcgg gaagactcga caggctttgt cgttgggggg gatgtaagcg aatggcaagt
     6421 ttgctcattg gaaacagtga gcggggcgcg aaatttttcg cgagctatga atacggggct
     6481 caatccaaac ttgcccggtc ctgaaaatca cgttagaacg cgtctgagcg cgtttttgat
     6541 gccatttaag gatgggaaac agggttggtg tcagaagcgc gctcaaaaaa aaaccccttg
     6601 gagcaggggg gcgaaaagac tatgcggaga ttatttgttg ttatgtgaac gccacatgaa
     6661 cataacatga tgtttcccat tttggctgga cttaaccttg ttttttttac ggttatctga
     6721 caaattgatt ccgttacatc gcgcaaaatg cgcattcctc ttgagtggtc catgaccggc
     6781 ccaaattttt tcatgcg
//
